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https://www.readbyqxmd.com/read/29793118/efficient-resource-recycling-from-liquid-digestate-by-microalgae-yeast-mixed-culture-and-the-assessment-of-key-gene-transcription-related-to-nitrogen-assimilation-in-microalgae
#1
Lei Qin, Lu Liu, Zhongming Wang, Weining Chen, Dong Wei
To determine the feasibility of microalgae-yeast mixed culture using the liquid digestate of dairy wastewater (LDDW) for biofuels and single cell protein (SCP) production, the cell growth, nutrient removal and outputs evaluation of the mono and mixed culture of Chlorella vulgaris and Yarrowia lipolytica in LDDW were investigated by adding glycerol as carbon source. The results showed that the mixed culture could enhance the biological utilization efficiency of nitrogen and phosphorus, and obtain higher yield of biomass (1...
May 18, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29791719/molecular-and-morphological-diversity-of-trebouxia-microalgae-in-sphaerothallioid-circinaria-spp-lichens
#2
Arántzazu Molins, Patricia Moya, Francisco J García-Breijo, José Reig-Armiñana, Eva Barreno
Three vagrant (Circinaria hispida, Circinaria gyrosa, Circinaria sp. 'paramerae') and one crustose (semi-vagrant, Circinaria sp.'oromediterranea') lichens growing in very continental areas in the Iberian Peninsula were selected to study the phycobiont diversity. Mycobiont identification was checked using nrITS DNA barcoding: Circinaria sp.'oromediterranea' and Circinaria sp. 'paramerae' formed a new clade. Phycobiont diversity was analyzed in 50 thalli of Circinaria spp. using nrITS DNA and LSU rDNA, with microalgae coexistence being found in all the species analyzed by Sanger sequencing...
May 23, 2018: Journal of Phycology
https://www.readbyqxmd.com/read/29790400/the-activity-of-silver-nanoparticles-against-microalgae-of-the-prototheca-genus
#3
Tomasz Jagielski, Zofia Bakuła, Małgorzata Pleń, Michał Kamiński, Julita Nowakowska, Jacek Bielecki, Krystyna I Wolska, Anna M Grudniak
AIM: To investigate the in vitro activity of silver NPs (AgNPs) against pathogenic microalgae of the Prototheca genus. MATERIALS & METHODS: The antialgal potential of AgNPs against Prototheca species of both clinical and environmental origin was assessed from minimum inhibitory (algistatic) and algicidal concentrations. The in vitro cytotoxicity of AgNPs against bovine mammary epithelial cell line was evaluated by means of the standard MTT assay. RESULTS: AgNPs showed a strong killing activity toward Prototheca algae, as the minimal algicidal concentration (MAC) values matched perfectly the corresponding minimum inhibitory concentration (MIC) values for all species (MAC = MIC, 1-4 mg/l), except P...
May 2018: Nanomedicine
https://www.readbyqxmd.com/read/29789928/effects-of-fundamental-nutrient-stresses-on-the-lipid-accumulation-profiles-in-two-diatom-species-thalassiosira-weissflogii-and-chaetoceros-muelleri
#4
Qun Lin, Wen-Hao Zhuo, Xin-Wei Wang, Chang-Ping Chen, Ya-Hui Gao, Jun-Rong Liang
Microalgae are considered as attractive feedstocks for biofuel production nowadays because of their high lipid contents and easy cultivation. In the present study, two diatoms, Thalassiosira weissflogii and Chaetoceros muelleri, were cultured under various nutrient-limitation conditions to explore their comprehensive lipid accumulation profiles for further commercialization. In T. weissflogii, the highest neutral lipid accumulation and highest lipid productivity (14.28 mg L-1  day-1 ) were both recorded under P-limitation...
May 22, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29785358/bacterial-community-changes-in-an-industrial-algae-production-system
#5
Scott P Fulbright, Adam Robbins-Pianka, Donna Berg-Lyons, Rob Knight, Kenneth F Reardon, Stephen T Chisholm
While microalgae are a promising feedstock for production of fuels and other chemicals, a challenge for the algal bioproducts industry is obtaining consistent, robust algae growth. Algal cultures include complex bacterial communities and can be difficult to manage because specific bacteria can promote or reduce algae growth. To overcome bacterial contamination, algae growers may use closed photobioreactors designed to reduce the number of contaminant organisms. Even with closed systems, bacteria are known to enter and cohabitate, but little is known about these communities...
April 2018: Algal Research
https://www.readbyqxmd.com/read/29783941/a-transposable-element-annotation-pipeline-and-expression-analysis-reveal-potentially-active-elements-in-the-microalga-tisochrysis-lutea
#6
Jérémy Berthelier, Nathalie Casse, Nicolas Daccord, Véronique Jamilloux, Bruno Saint-Jean, Grégory Carrier
BACKGROUND: Transposable elements (TEs) are mobile DNA sequences known as drivers of genome evolution. Their impacts have been widely studied in animals, plants and insects, but little is known about them in microalgae. In a previous study, we compared the genetic polymorphisms between strains of the haptophyte microalga Tisochrysis lutea and suggested the involvement of active autonomous TEs in their genome evolution. RESULTS: To identify potentially autonomous TEs, we designed a pipeline named PiRATE (Pipeline to Retrieve and Annotate Transposable Elements, download: https://doi...
May 22, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29783193/quorum-sensing-molecules-in-activated-sludge-could-trigger-microalgae-lipid-synthesis
#7
Chaofan Zhang, Qingcheng Li, Liang Fu, Dandan Zhou, John C Crittenden
Cultivating microalgae using wastewater is an economical strategy to produce biofuel; however, microbial contamination has to be controlled strictly. Microalgae lipid accumulation can be triggered by environmental pressures, and here, we studied whether microbial contamination is the pressure for microalgae. We hypothesized this pressure was forced via cell-to-cell communication with quorum sensing molecules (QSMs). In this work, we verified the impacts of QSMs produced by activated sludge (wastewater-born microbial consortiums) on both lipid content and biomass production of the microalgae Chlorophyta sp...
May 18, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29783132/characterization-of-biomass-waste-torrefaction-under-conventional-and-microwave-heating
#8
Shih-Hsin Ho, Congyu Zhang, Wei-Hsin Chen, Ying Shen, Jo-Shu Chang
To evaluate the potential of microwave heating for biomass torrefaction, the torrefaction performances and energy utilization of coffee grounds and microalga residue, under conventional and microwave heating were investigated and compared with each other. For the two biomass samples, the dehydrogenation of the coffee grounds was more sensitive to torrefaction severity, whereas the microalga residue consumed more energy under the same torrefaction conditions. Microwave heating under lower torrefaction severity had a higher energy efficiency...
May 13, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29779121/a-comprehensive-comparable-study-of-the-physiological-properties-of-four-microalgal-species-under-different-light-wavelength-conditions
#9
Yu Zhong, Peng Jin, Jay J Cheng
Microalgae treated with blue light have potential for production of human nutrition supplement and biofuel due to their higher biomass productivity and favorable fatty acid composition. Chlorella vulgaris, Chlorella pyrenoidosa, Scenedesmus quadricauda and Scenedesmus obliquus are representative green microalgae which are widely reported for algal production. In this study, we provide a systematic investigation of the biomass productivity, photosynthetic pigments, chlorophyll fluorescence and fatty acid content of the four green microalgae...
May 19, 2018: Planta
https://www.readbyqxmd.com/read/29778794/effect-of-nitrogen-limitation-on-biochemical-composition-and-photosynthetic-performance-for-fed-batch-mixotrophic-cultivation-of-microalga-spirulina-platensis
#10
Xiaoting Li, Wei Li, Jun Zhai, Haoxuan Wei
In this study, the effect of nitrogen limitation on microalgal growth, biochemical composition and photosynthetic performance was investigated in fed-batch mixotrophic cultivation of microalga Spirulina platensis, compared with that in autotrophic cultivation. The microalgal biomass productivity was greatly enhanced by mixotrophic cultivation. With nitrogen limitation, the mixotrophic culture accelerated the degradation of microalgal pigments and proteins to supply intracellular nitrogen for maintaining higher biomass productivity, simultaneously accumulating more carbohydrates...
May 12, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29778594/differetial-toxin-profiles-of-ciguatoxins-in-marine-organisms-chemistry-fate-and-global-distribution
#11
REVIEW
Lucía Soliño, Pedro Reis Costa
Ciguatoxins (CTXs) are fish metabolism products and a result of biotransformation of precursor gambiertoxins produced, in the first instance, by benthic dinoflagellates Gambierdiscus and Fukuyoa. Ciguatoxins are potent neurotoxins that selectively open voltage gated sodium channels in excitable cells causing the human food poisoning known as Ciguatera (CFP). Endemic from tropical areas in central Pacific and West Indian Ocean, and the Caribbean Sea, CTX may affects up to 500,000 people annually due to fish consumption...
May 17, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29778229/a-novel-portable-filtration-system-for-sampling-and-concentration-of-microorganisms-demonstration-on-marine-microalgae-with-subsequent-quantification-using-ic-nasba
#12
Christos-Moritz Loukas, Matthew C Mowlem, Maria-Nefeli Tsaloglou, Nicolas G Green
This paper presents a novel portable sample filtration/concentration system, designed for use on samples of microorganisms with very low cell concentrations and large volumes, such as water-borne parasites, pathogens associated with faecal matter, or toxic phytoplankton. The example application used for demonstration was the in-field collection and concentration of microalgae from seawater samples. This type of organism is responsible for Harmful Algal Blooms (HABs), an example of which is commonly referred to as "red tides", which are typically the result of rapid proliferation and high biomass accumulation of harmful microalgal species in the water column or at the sea surface...
May 2018: Harmful Algae
https://www.readbyqxmd.com/read/29778220/development-of-a-qpcr-assay-to-detect-and-quantify-ichthyotoxic-flagellates-along-the-norwegian-coast-and-the-first-norwegian-record-of-fibrocapsa-japonica-raphidophyceae
#13
Anette Engesmo, David Strand, Sandra Gran-Stadniczeñko, Bente Edvardsen, Linda K Medlin, Wenche Eikrem
Blooms of ichthyotoxic microalgae pose a great challenge to the aquaculture industry world-wide, and there is a need for fast and specific methods for their detection and quantification in monitoring programs. In this study, quantitative real-time PCR (qPCR) assays for the detection and enumeration of three ichthyotoxic flagellates: the dinoflagellate Karenia mikimotoi (Miyake & Kominami ex Oda) Hansen & Moestrup and the two raphidophytes Heterosigma akashiwo (Hada) Hada ex Hara & Chihara and Fibrocapsa japonica Toriumi & Takano were developed...
May 2018: Harmful Algae
https://www.readbyqxmd.com/read/29778023/the-growth-and-lipid-accumulation-of-scenedesmus-quadricauda-during-batch-mixotrophic-heterotrophic-cultivation-using-xylose-as-a-carbon-source
#14
Mingming Song, Haiyan Pei
To overcome the bottlenecks of high cost and low production yields that restrict the commercial production of microalgae biodiesel, the use of xylose was evaluate by Scenedesmus quadricauda FACHB-1297, which was shown to be capable of mixotrophic and heterotrophic growth and lipid production on xylose, rich in the waste streams from pulp and paper industry, with increases in lipid productivities of 35.8-fold (mixotrophic) and 9.2-fold (heterotrophic) in comparison to photoautotrophic lipid yields. Five doses of xylose were tested to determine the effects and mechanisms of the carbon source on microalgae in mixotrophic mode...
May 10, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29777738/establishment-of-an-efficient-genetic-transformation-method-in-dunaliella-tertiolecta-mediated-by-agrobacterium-tumefaciens
#15
Claudia D Norzagaray-Valenzuela, Lourdes J Germán-Báez, Marco A Valdez-Flores, Sergio Hernández-Verdugo, Luke M Shelton, Angel Valdez-Ortiz
Microalgae are photosynthetic microorganisms widely used for the production of highly valued compounds, and recently they have been shown to be promising as a system for the heterologous expression of proteins. Several transformation methods have been successfully developed, from which the Agrobacterium tumefaciens-mediated method remains the most promising. However, microalgae transformation efficiency by A. tumefaciens is shown to vary depending on several transformation conditions. The present study aimed to establish an efficient genetic transformation system in the green microalgae Dunaliella tertiolecta using the A...
May 16, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29776927/ecological-engineering-helps-maximize-function-in-algal-oil-production
#16
Sara L Jackrel, Anita Narwani, Bastian Bentlage, Robert B Levine, David C Hietala, Phillip E Savage, Todd H Oakley, Vincent J Denef, Bradley J Cardinale
Algal biofuels have the potential to curb emissions of greenhouse gases from fossil fuels, but current growing methods fail to produce fuels that meet the multiple standards necessary for economical industrial use. For example, algae grown as monocultures for biofuel production have not simultaneously and economically achieved high yields of the high-quality, lipid-rich biomass desired for the industrial-scale production of bio-oil. Decades of study in the field of ecology have demonstrated that simultaneous increases in multiple functions, such as the quantity and quality of biomass, can occur in natural ecosystems by increasing biological diversity...
May 18, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29776459/phycoremediation-of-municipal-wastewater-by-the-cold-adapted-microalga-monoraphidium-sp-dek19
#17
(no author information available yet)
Present municipal wastewater treatment technologies often require substantial energy inputs, and fail to completely remove nitrate and phosphate before discharging effluent. In contrast, utilizing the cold-adapted oleaginous microalga Monoraphidium sp. Dek 19 decreased levels of both these polluting ions to 0 mg/dl. Concurrent biomass production was greater at 10oC than at 22 oC, showing that phycoremediation occurred at low temperatures previously thought to be unsuitable for algal-based treatment. Algal growth with uptake of nitrate and phosphate required only short bursts of aeration to suspend cells and maintain CO2 supply for photosynthesis...
May 8, 2018: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/29773893/co-cultivation-of-fungal-microalgal-strains-in-biogas-slurry-and-biogas-purification-under-different-initial-co-2-concentrations
#18
Kai Zhou, Yuejin Zhang, Xiaobo Jia
The effects of five different microalgae-fungi on nutrient removal and CO2 removal were investigated under three different CO2 contents (35%, 45% and 55%). The results showed that the highest nutrient and CO2 removal efficiency were found at 55% CO2 by cocultivation of different microalgae and fungi. The effect of different initial CO2 concentration on the removal of CO2 from microalgae was significant, and the order of CO2 removal efficiency was 55% (v/v) >45% (v/v) >35% (v/v). The best nutrient removal and biogas purification could be achieved by co-cultivation of C...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29772529/microbial-bio-fuels-a-solution-to-carbon-emissions-and-energy-crisis
#19
Arun Kumar, Sumit Kaushal, Shubhini A Saraf, Jay Shankar Singh
Increasing energy demand, limited fossil fuel resources and climate change have prompted development of alternative sustainable and economical fuel resources such as crop-based bio-ethanol and bio-diesel. However, there is concern over use of arable land that is used for food agriculture for creation of biofuel. Thus, there is a renewed interest in the use of microbes particularly microalgae for bio-fuel production. Microbes such as micro-algae and cyanobacteria that are used for biofuel production also produce other bioactive compounds under stressed conditions...
June 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/29768936/nutrient-removal-and-biomass-production-advances-in-microalgal-biotechnology-for-wastewater-treatment
#20
Sudharsanam Abinandan, Suresh R Subashchandrabose, Kadiyala Venkateswarlu, Mallavarapu Megharaj
Owing to certain drawbacks, such as energy-intensive operations in conventional modes of wastewater treatment (WWT), there has been an extensive search for alternative strategies in treatment technology. Biological modes for treating wastewaters are one of the finest technologies in terms of economy and efficiency. An integrated biological approach with chemical flocculation is being conventionally practiced in several-sewage and effluent treatment plants around the world. Overwhelming responsiveness to treat wastewaters especially by using microalgae is due to their simplest photosynthetic mechanism and ease of acclimation to various habitats...
May 17, 2018: Critical Reviews in Biotechnology
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