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https://www.readbyqxmd.com/read/28651134/effect-of-salt-type-and-concentration-on-the-growth-and-lipid-content-of-chlorella-vulgaris-in-synthetic-saline-wastewater-for-biofuel-production
#1
Jared Church, Jae-Hoon Hwang, Keug-Tae Kim, Rebecca McLean, You-Kwan Oh, Bora Nam, Jin Chul Joo, Woo Hyoung Lee
Microalgae can offer several benefits for wastewater treatment with their ability to produce large amounts of lipids for biofuel production and the high economic value of harvested biomass for biogas and fertilizer. This study found that salt concentration (∼45gL(-1)) had more of an effect than salt type on metabolisms of Chlorella vulgaris for wastewater treatment and biofuel production. Salinity stress decreased the algal growth rate in wastewater by 0.003day(-1)permScm(-1) and slightly reduced nutrient removal rates...
June 17, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28650572/cloning-and-characterization-of-nitrate-reductase-gene-in-ulva-prolifera-ulvophyceae-chlorophyta
#2
Yang Guo, Hao Zhe Wang, Chun Hui Wu, Hui Hui Fu, Peng Jiang
Ulva spp. dominates green tides around the world, which are occurring at an accelerated rate. The competitive nitrogen assimilation efficiency in Ulva is suggested to result in ecological success against other seaweeds. However, molecular characterization of genes involved in nitrogen assimilation have not been conducted. Here, we describe the identification of the nitrate reductase (NR) gene from a green seaweed Ulva prolifera, an alga which is responsible for the world's largest green tide in the Yellow Sea...
June 26, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/28649761/functional-assessment-of-plant-and-microalgal-lipid-pathway-genes-in-yeast-to-enhance-microbial-industrial-oil-production
#3
Huadong Peng, Lalehvash Moghaddam, Anthony Brinin, Brett Williams, Sagadevan Mundree, Victoria S Haritos
As promising alternatives to fossil-derived oils, microbial lipids are important as industrial feedstocks for biofuels and oleochemicals. Our broad aim is to increase lipid content in oleaginous yeast through expression of lipid accumulation genes and use Saccharomyces cerevisiae to functionally assess genes obtained from oil-producing plants and microalgae. Lipid accumulation genes DGAT (diacylglycerol acyltransferase), PDAT (phospholipid: diacylglycerol acyltransferase) and ROD1 (phosphatidylcholine: diacylglycerol choline-phosphotransferase) were separately expressed in yeast and lipid production measured by fluorescence, solvent extraction, TLC and GC of fatty acid methyl esters...
June 25, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28649685/templated-and-self-limiting-calcite-formation-directed-by-coccolith-organic-macromolecules
#4
Assaf Gal, Richard Wirth, Zahava Barkay, Noam Eliaz, André Scheffel, Damien Faivre
The formation of intricately shaped crystalline minerals by organisms is orchestrated by specialized biomacromolecules. The macromolecules associated with coccoliths, nanometer-sized calcite crystal arrays produced by marine microalgae, can form a distinct calcium-rich phase via macromolecular recognition. Here, we show that this calcium-rich phase can be mineralized into a thin film of single-crystalline calcite by the balanced addition of carbonate ions. Such a crystallization process provides a strategy to direct crystalline products via local interactions between soluble macromolecules and compatible templates...
June 26, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28649474/using-high-throughput-sequencing-of-its2-to-describe-symbiodinium-metacommunities-in-st-john-us-virgin-islands
#5
Ross Cunning, Ruth D Gates, Peter J Edmunds
Symbiotic microalgae (Symbiodinium spp.) strongly influence the performance and stress-tolerance of their coral hosts, making the analysis of Symbiodinium communities in corals (and metacommunities on reefs) advantageous for many aspects of coral reef research. High-throughput sequencing of ITS2 nrDNA offers unprecedented scale in describing these communities, yet high intragenomic variability at this locus complicates the resolution of biologically meaningful diversity. Here, we demonstrate that generating operational taxonomic units by clustering ITS2 sequences at 97% similarity within, but not across, samples collapses sequence diversity that is more likely to be intragenomic, while preserving diversity that is more likely interspecific...
2017: PeerJ
https://www.readbyqxmd.com/read/28648747/anaerobic-co-digestion-of-chicken-manure-and-microalgae-chlorella-sp-methane-potential-microbial-diversity-and-synergistic-impact-evaluation
#6
Ruirui Li, Na Duan, Yuanhui Zhang, Zhidan Liu, Baoming Li, Dongming Zhang, Taili Dong
Anaerobic digestion (AD) is a promising alternative for livestock manure management. This paper presents the experimental results obtained through a batch experiment by using chicken manure (CM) and microalgae Chlorella sp. as co-substrates. The effect of co-digestion was evaluated by varying CM to Chlorella sp. ratios (0:10, 2:8, 4:6, 6:4, 8:2, 10: 0 based on the volatile solids (VS)). The major objective of this study is to evaluate the feasibility and synergistic impact of co-digestion of CM and Chlorella sp...
June 22, 2017: Waste Management
https://www.readbyqxmd.com/read/28647795/investigation-of-chlorella-vulgaris-utex-265-cultivation-under-light-and-low-temperature-stressed-conditions-for-lutein-production-in-flasks-and-the-coiled-tree-photo-bioreactor-ctpbr
#7
Mengyue Gong, Amarjeet Bassi
Lutein has an increasing share in the pharmaceutical and nutraceutical market due to its benefits to eye health. Microalgae may be a potential source for lutein production while the expense limits the commercialization. In this study, a coiled tubular tree photobioreactor (CTPBR) design was investigated for cultivating the cold tolerant microalgae Chlorella vulgaris UTEX 265 under various conditions for lutein production. The influence and interaction of light irradiance strength, lighting cycle, and temperature on microalgae and lutein production efficiency at low temperature range were also studied in flasks via response surface method (RSM)...
June 24, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28647321/development-of-large-scale-and-economic-ph-control-system-for-outdoor-cultivation-of-microalgae-haematococcus-pluvialis-using-industrial-flue-gas
#8
Yoon Young Choi, Jae Min Joun, Jeewon Lee, Min Eui Hong, Hoang-Minh Pham, Won Seok Chang, Sang Jun Sim
The aim of this study was to develop the economic and effective buffer system for microalgae mass cultivation using industrial flue gas. Due to the continuous flue gas supplement, culture media acidified, therefore cell growth inhibited. Although buffering agent was added, this result increase in cost for overall culture process. Therefore combined buffer system of bicarbonate and phosphate (BP) for large-scale use was investigated. The bicarbonate buffer system generated from CO2 dissolution, additionally phosphate buffer system improves the buffer performance under the continuous CO2 supplementation from flue gas...
May 26, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28647256/bioavailability-of-wastewater-derived-dissolved-organic-nitrogen-to-green-microalgae-selenastrum-capricornutum-chlamydomonas-reinhardtii-and-chlorella-vulgaris-with-without-presence-of-bacteria
#9
Jingyi Sun, Halis Simsek
Effluent dissolved organic nitrogen (DON) is problematic in nutrient sensitive surface waters and needs to be reduced to meet demanding total dissolved nitrogen discharge limits. Bioavailable DON (ABDON) is a portion of DON utilized by algae or algae+bacteria, while biodegradable DON (BDON) is a portion of DON decomposable by bacteria. ABDON and BDON in a two-stage trickling filter (TF) wastewater treatment plant was evaluated using three different microalgal species, Selenastrum capricornutum, Chlamydomonas reinhardtii and Chlorella vulgaris and mixed cultured bacteria...
July 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28644993/long-term-toxicity-of-surface-charged-polystyrene-nanoplastics-to-marine-planktonic-species-dunaliella-tertiolecta-and-artemia-franciscana
#10
E Bergami, S Pugnalini, M L Vannuccini, L Manfra, C Faleri, F Savorelli, K A Dawson, I Corsi
Plastic pollution has been globally recognized as a critical issue for marine ecosystems and nanoplastics constitute one of the last unexplored areas to understand the magnitude of this threat. However, current difficulties in sampling and identifying nano-sized debris make hard to assess their occurrence in marine environment. Polystyrene nanoparticles (PS NPs) are largely used as nanoplastics in ecotoxicological studies and although acute exposures have been already investigated, long-term toxicity on marine organisms is unknown...
June 15, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28644408/isolation-and-selection-of-microalgal-strains-from-natural-water-sources-in-viet-nam-with-potential-for-edible-oil-production
#11
Tran Yen Thao, Dinh Thi Nhat Linh, Vo Chi Si, Taylor W Carter, Russell T Hill
Industrial vegetable oil production in Viet Nam depends on oil seeds and crude plant oils that are currently more than 90% imported. As the first step in investigating the feasibility of using microalgae to provide Viet Nam with a domestic source of oil for food and edible oil industries, fifty lipid-producing microalgae were isolated and characterized. The microalgae were isolated from water sources ranging from freshwater to brackish and marine waters from a wide geographic distribution in Viet Nam. Initial analyses showed that 20 of the 50 strains had good growth rates, produced high biomass and had high lipid content, ranging up to 50% of dry weight biomass...
June 23, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28642053/from-low-cost-substrates-to-single-cell-oils-synthesized-by-oleaginous-yeasts
#12
REVIEW
Lei Qin, Lu Liu, An-Ping Zeng, Dong Wei
As new feedstock for biofuels, microbial oils have received worldwide attentions due to their environmentally-friendly characters. Microbial oil production based on low-cost raw materials is significantly attractive to the current biodiesel refinery industry. In terms of SCOs production, oleaginous yeast has numerous advantages over bacteria, molds and microalgae based on their high growth rate and lipid yield. Numerous efforts have been made on the competitive lipid production combining the use of cheap raw materials as substrates by yeasts...
May 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28641207/ros-and-calcium-signaling-mediated-pathways-involved-in-stress-responses-of-the-marine-microalgae-dunaliella-salina-to-enhanced-uv-b-radiation
#13
Xinxin Zhang, Xuexi Tang, Ming Wang, Wei Zhang, Bin Zhou, You Wang
UV-B ray has been addressed to trigger common metabolic responses on marine microalgae, however, the upstream events responsible for these changes in marine microalgae are poorly understood. In the present study, a species of marine green microalgae Dunaliella salina was exposed to a series of enhanced UV-B radiation ranging from 0.25 to 1.00 KJ·m(-2) per day. The role of ROS and calcium signaling in the D. salina responses to UV-B was discussed. Results showed that enhanced UV-B radiation markedly decreased the cell density in a dose-dependent manner, but the contents of protein and glycerol that were essential for cell growth increased...
May 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28639019/cultivation-of-freshwater-microalgae-in-biodiesel-wash-water
#14
Patrícia Giulianna Petraglia Sassi, Clediana Dantas Calixto, Jordana Kaline da Silva Santana, Roberto Sassi, Cristiane Francisca Costa Sassi, Raphael Abrahão
Biodiesel wash water is a contaminating industrial effluent that must be treated prior to disposal. The use of this effluent as a low-cost alternative cultivation medium for microalgae could represent a viable supplementary treatment. We cultivated 11 microalgae species with potential use for biodiesel production to assess their growth capacities in biodiesel industrial washing waters. Only Monoraphidium contortum, Ankistrodesmus sp., Chlorococcum sp., and one unidentified Chlorophyceae species grew effectively in that effluent...
June 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28638375/valorization-of-spent-escherichia-coli-media-using-green-microalgae-chlamydomonas-reinhardtii-and-feedstock-production
#15
Jian-Guo Zhang, Fang Zhang, Kiran Thakur, Fei Hu, Zhao-Jun Wei
The coupling of Chlamydomonas reinhardtii biomass production for nutrients removal of Escherichia coli anaerobic broth (EAB) is thought to be an economically feasible option for the cultivation of microalgae. The feasibility of growing microalgae in using EAB high in nutrients for the production of more biomass was examined. EAB comprised of nutrient-abundant effluents, which can be used to produce microalgae biomass and remove environment pollutant simultaneously. In this study, C. reinhardtii 21gr (cc1690) was cultivated in different diluted E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28638272/bioactivities-of-ethanol-extract-from-the-antarctic-freshwater-microalga-chloromonas-sp
#16
Sung-Suk Suh, Eun Jin Yang, Sung Gu Lee, Ui Joung Youn, Se Jong Han, Il-Chan Kim, Sanghee Kim
Cancer is the principal cause of human death and occurs through highly complex processes that involve the multiple coordinated mechanisms of tumorigenesis. A number of studies have indicated that the microalgae extracts showed anticancer activity in a variety of human cancer cells and can provide a new insight in the development of novel anti-cancer therapy. Here, in order to investigate molecular mechanisms of anticancer activity in the Antarctic freshwater microalga, Chloromonas sp., we prepared ethanol extract of Chloromonas sp...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28637160/energy-producing-electro-flocculation-for-harvest-of-dunaliella-salina
#17
Qing Liu, Meng Zhang, Tao Lv, Hongjun Chen, Anthony Okonkwo Chika, Changli Xiang, Minxue Guo, Minghui Wu, Jianjun Li, Lishan Jia
In this study, an efficient electro-flocculation process for Dunaliella salina with energy production by aluminum-air battery has been successfully applied. The formed aluminum hydroxide hydrates during discharging of battery were positively charged, which have a great potential for microalgae flocculation. The precipitation of aluminum hydroxide hydrates by algae also could improve the performance of aluminum-air battery. The harvesting efficiency could reach 97% in 20mins with energy production of 0.11kWh/kg...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28636352/prevention-of-marine-biofouling-using-the-natural-allelopathic-compound-batatasin-iii-and-synthetic-analogues
#18
Lindon W K Moodie, Rozenn Trepos, Gunnar Cervin, Kari Anne Bråthen, Bente Lindgård, Rigmor Reiersen, Patrick Cahill, Henrik Pavia, Claire Hellio, Johan Svenson
The current study reports the first comprehensive evaluation of a class of allelopathic terrestrial natural products as antifoulants in a marine setting. To investigate the antifouling potential of the natural dihydrostilbene scaffold, a library of 22 synthetic dihydrostilbenes with varying substitution patterns, many of which occur naturally in terrestrial plants, were prepared and assessed for their antifouling capacity. The compounds were evaluated in an extensive screen against 16 fouling marine organisms...
June 21, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28635649/the-sea-urchin-arbacia-lixula-a-novel-natural-source-of-astaxanthin
#19
Paola Cirino, Christophe Brunet, Martina Ciaravolo, Christian Galasso, Luigi Musco, Tomás Vega Fernández, Clementina Sansone, Alfonso Toscano
Several echinoderms, including sea urchins, are valuable sources of bioactive compounds but their nutraceutical potential is largely unexplored. In fact, the gonads of some sea urchin species contain antioxidants including carotenoids and polyhydroxylated naphthoquinones (PHNQ's), such as echinochrome A. Astaxanthin is known to have particular bioactivity for the prevention of neurodegenerative diseases. This carotenoid is produced by microalgae, while several marine invertebrates can bioaccumulate or synthetize it from metabolic precursors...
June 21, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28634846/lipid-accumulation-and-metabolic-analysis-based-on-transcriptome-sequencing-of-filamentous-oleaginous-microalgae-tribonema-minus-at-different-growth-phases
#20
Hui Wang, Lili Gao, Huimin Shao, Wenjun Zhou, Tianzhong Liu
Filamentous oleaginous microalgae specie Tribonema minus is a promising feedstock for biodiesel production. However, the metabolic mechanism of lipid production in this filamentous microalgal specie remains unclear. Here, we compared the lipid accumulation of T. minus at different growth phases, and described the de novo transcriptome sequencing and assembly and identified important pathways and genes involved in TAG production. Total lipid increased by 2.5-fold and its TAG level in total lipid reached 81.1% at stationary phase...
June 20, 2017: Bioprocess and Biosystems Engineering
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