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https://www.readbyqxmd.com/read/28231253/community-analysis-of-pigment-patterns-from-37-microalgae-strains-reveals-new-carotenoids-and-porphyrins-characteristic-of-distinct-strains-and-taxonomic-groups
#1
Benoît Serive, Elodie Nicolau, Jean-Baptiste Bérard, Raymond Kaas, Virginie Pasquet, Laurent Picot, Jean-Paul Cadoret
Phytoplankton, with an estimated 30 000 to 1 000 000 species clustered in 12 phyla, presents a high taxonomic and ecophysiological diversity, reflected by the complex distribution of pigments among the different algal classes. High performance liquid chromatography is the gold standard method for qualitative and quantitative analysis of phytoplankton pigments in seawater and culture samples, but only a few pigments can be used as robust chemotaxonomic markers. A major challenge is thus to identify new ones, characteristic of a strain, species, class or taxon that cannot be currently identified on the basis of its pigment signature...
2017: PloS One
https://www.readbyqxmd.com/read/28231149/microalgae-nutraceuticals
#2
REVIEW
Marcello Nicoletti
Among the new entries in the food supplements sector, an important place must be assigned to nutraceuticals containing microalgae, nowadays accounting for a large and rapidly expanding market. The marketed products are mainly based on three production strains, i.e., Spirulina and Chlorella, followed at a distance by Klamath. It is a composite situation, since two of them are cyanobacteria and the second one is eukaryotic. The reality is that each presents similarities in shape and appearance concerning the marketed form and several utilizations, and peculiarities that need special attention and adequate studies...
August 22, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28228843/a-new-mechanism-for-efficient-hydrocarbon-electro-extraction-from-botryococcus-braunii
#3
Alexis Guionet, Bahareh Hosseini, Justin Teissié, Hidenori Akiyama, Hamid Hosseini
BACKGROUND: Recent understanding that specific algae have high hydrocarbon production potential has attracted considerable attention. Botryococcus braunii is a microalga with an extracellular hydrocarbon matrix, which makes it an appropriate green energy source. RESULTS: This study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to create electro-permeabilization. In such a way, technical limitations due to high extraction energy and cost can be overcome...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28228091/exploring-the-transcriptome-of-non-model-oleaginous-microalga-dunaliella-tertiolecta-through-high-throughput-sequencing-and-high-performance-computing
#4
Lina Yao, Kenneth Wei Min Tan, Tin Wee Tan, Yuan Kun Lee
BACKGROUND: RNA-Seq technology has received a lot of attention in recent years for microalgal global transcriptomic profiling. It is widely used in transcriptome-wide analysis of gene expression., particularly for microalgal strains with potential as biofuel sources. However, insufficient genomic or transcriptomic information of non-model microalgae has limited the understanding of their regulatory mechanisms and hampered genetic manipulation to enhance biofuel production. As such, an optimal microalgal transcriptomic database construction is a subject of urgent investigation...
February 22, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28222675/identification-and-characterization-of-an-efficient-acyl-coa-diacylglycerol-acyltransferase-1-dgat1-gene-from-the-microalga-chlorella-ellipsoidea
#5
Xuejie Guo, Chengming Fan, Yuhong Chen, Jingqiao Wang, Weibo Yin, Richard R C Wang, Zanmin Hu
BACKGROUND: Oil in the form of triacylglycerols (TAGs) is quantitatively the most important storage form of energy for eukaryotic cells. Diacylglycerol acyltransferase (DGAT) is considered the rate-limiting enzyme for TAG accumulation. Chlorella, a unicellular eukaryotic green alga, has attracted much attention as a potential feedstock for renewable energy production. However, the function of DGAT1 in Chlorella has not been reported. RESULTS: A full-length cDNA encoding a putative diacylglycerol acyltransferase 1 (DGAT1, EC 2...
February 21, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28222383/simultaneous-biogas-upgrading-and-centrate-treatment-in-an-outdoors-pilot-scale-high-rate-algal-pond
#6
Esther Posadas, David Marín, Saúl Blanco, Raquel Lebrero, Raúl Muñoz
The bioconversion of biogas to biomethane coupled to centrate treatment was evaluated in an outdoors pilot scale high rate algal pond interconnected to an external CO2-H2S absorption column (AC) via settled broth recirculation. CO2-removal efficiencies ranged from 50 to 95% depending on the alkalinity of the cultivation broth and environmental conditions, while a complete H2S removal was achieved regardless of the operational conditions. A maximum CH4 concentration of 94% with a limited O2 and N2 stripping was recorded in the upgraded biogas at recycling liquid/biogas ratios in the AC of 1 and 2...
February 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28221783/cultivation-of-the-marine-macroalgae-chaetomorpha-linum-in-municipal-wastewater-for-nutrient-recovery-and-biomass-production
#7
Shijian Ge, Pascale Champagne
Compared to microalgae, macroalgae are larger in size, thereby imposing lower separation and drying costs. This study demonstrates the feasibility of cultivating macroalgae Chaetomorpha linum (C. linum) in different types of municipal wastewaters, their ability to remove nutrient and their biomass composition for downstream biofuel production. Screening experiments indicated that C. linum grew well on primary (PW) and secondary wastewaters (SW), as well as centrate wastewater (CW) diluted to less than 20%. In a subsequent experiment, a step feeding approach was found to significantly increase biomass productivity to 10...
February 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28220855/extending-calibration-free-force-measurements-to-optically-trapped-rod-shaped-samples
#8
Frederic Català, Ferran Marsà, Mario Montes-Usategui, Arnau Farré, Estela Martín-Badosa
Optical trapping has become an optimal choice for biological research at the microscale due to its non-invasive performance and accessibility for quantitative studies, especially on the forces involved in biological processes. However, reliable force measurements depend on the calibration of the optical traps, which is different for each experiment and hence requires high control of the local variables, especially of the trapped object geometry. Many biological samples have an elongated, rod-like shape, such as chromosomes, intracellular organelles (e...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28219380/effects-of-nutritional-conditions-on-growth-and-biochemical-composition-of-tetraselmis-sp
#9
Mouna Dammak, Bilel Hadrich, Ramzi Miladi, Mohamed Barkallah, Faiez Hentati, Ridha Hachicha, Céline Laroche, Philippe Michaud, Imen Fendri, Slim Abdelkafi
BACKGROUND: This study aimed to maximize biomass concentration, biomass productivity and biochemical composition of the marine microalga Tetraselmis sp. METHODS: In the current study, Box-Behnken Design was used to model the effect of NaNO3, NaH2PO4, metals and vitamins in the F/2 medium on the growth, total chlorophylls, carotenoids and starch contents. The total chlorophylls content was quantified by spectrophotometry. The FT-IR spectroscopy was used to estimate the biochemical compositions of Tetraselmis sp...
February 20, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28219051/simultaneous-cell-disruption-and-lipid-extraction-in-a-microalgal-biomass-using-a-nonpolar-tertiary-amine
#10
Wen-Can Huang, Jong-Duk Kim
A simultaneous cell disruption and lipid extraction method is developed for microalgal biodiesel production using a triethylamine/methanol solvent system. Individually, the pure solvents, i.e. triethylamine and methanol, do not exhibit significant enhancement in lipid extraction, but a 3:7 (v/v) triethylamine/methanol mixture exhibits the highest lipid extraction, corresponding to 150% of the conventional chloroform/methanol (2:1, v/v) solvent extraction. This extraction is equivalent to 92.5% of the total lipids, even when extracted from a wet microalgal biomass with a water content of 80%...
February 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28218992/differently-localized-lysophosphatidic-acid-acyltransferases-crucial-for-triacylglycerol-biosynthesis-in-the-oleaginous-alga-nannochloropsis
#11
Takashi Nobusawa, Koichi Hori, Hiroshi Mori, Ken Kurokawa, Hiroyuki Ohta
Production of renewable bioenergy will be necessary to meet rising global fossil fuel demands. Members of the marine microalgae genus Nannochloropsis produce large amounts of oils (triacylglycerols; TAGs), and this genus is regarded as one of the most promising for biodiesel production. Recent genome sequencing and transcriptomic studies on Nannochloropsis have provided a foundation for understanding its oleaginous trait, but the mechanism underlying oil accumulation remains to be clarified. Here we report Nannochloropsis knockout strains of four extraplastidic lysophosphatidic acid acyltransferases (LPAT1-4), which catalyze a major de novo biosynthetic step of TAGs and membrane lipids...
February 20, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28217746/integrated-regulatory-and-metabolic-networks-of-the-marine-diatom-phaeodactylum-tricornutum-predict-the-response-to-rising-co2-levels
#12
Jennifer Levering, Christopher L Dupont, Andrew E Allen, Bernhard O Palsson, Karsten Zengler
Diatoms are eukaryotic microalgae that are responsible for up to 40% of the ocean's primary productivity. How diatoms respond to environmental perturbations such as elevated carbon concentrations in the atmosphere is currently poorly understood. We developed a transcriptional regulatory network based on various transcriptome sequencing expression libraries for different environmental responses to gain insight into the marine diatom's metabolic and regulatory interactions and provide a comprehensive framework of responses to increasing atmospheric carbon levels...
January 2017: MSystems
https://www.readbyqxmd.com/read/28214444/the-boosted-biomass-and-lipid-accumulation-in-chlorella-vulgaris-by-supplementation-of-synthetic-phytohormone-analogs
#13
Tingting Liu, Fei Liu, Chao Wang, Zhenyao Wang, Yuqin Li
This study attempted at maximizing biomass and lipid accumulation in Chlorella vulgaris by supplementation of natural abscisic acid (ABA) or synthetic 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) hormone analogs. Amongst three tested additives, NAA-treatment performed remarkable promoting effect on cell growth and lipid biosynthesis. The favorable lipid productivity (418.6mg/L/d) of NAA-treated cells showed 1.48 and 2.24 times more than that of 2,4-D and ABA. NAA-treatment also positively modified the proportions of saturated (C16:0 and C18:0) and monounsaturated fatty acids (C18:1) which were prone to high-quality biofuels-making...
February 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28213735/microalgae-based-advanced-municipal-wastewater-treatment-for-reuse-in-water-bodies
#14
REVIEW
Jing-Han Wang, Tian-Yuan Zhang, Guo-Hua Dao, Xue-Qiao Xu, Xiao-Xiong Wang, Hong -Ying Hu
Reuse of secondary municipal effluent from wastewater treatment plants in water bodies could effectively alleviate freshwater resource shortage. However, excessive nutrients must be efficiently removed to prevent eutrophication. Compared with other means of advanced wastewater treatment, microalgae-based processes display overwhelming advantages including efficient and simultaneous N and P removal, no requirement of additional chemicals, O2 generation, CO2 mitigation, and potential value-added products from harvested biomass...
February 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28212387/zooxanthellal-genetic-varieties-in-giant-clams-are-partially-determined-by-species-intrinsic-and-growth-related-characteristics
#15
Shota Ikeda, Hiroshi Yamashita, Shi-Nobu Kondo, Ken Inoue, Shin-Ya Morishima, Kazuhiko Koike
Giant clams (tridacnine shellfishes) are large bivalves that inhabit tropical and subtropical waters and harbor the symbiotic microalgae zooxanthellae, which consist of diverse phylotypes (clades). Each clade exhibits unique physiological characteristics, and the cladal composition may influence the host's survival and its ability to tolerate environmental changes. Using quantitative PCR (qPCR) assays, we investigated the zooxanthellal genetic clades in Tridacna crocea (n = 93) and Tridacna squamosa (n = 93)...
2017: PloS One
https://www.readbyqxmd.com/read/28210816/efficient-solvothermal-wet-in-situ-transesterification-of-nannochloropsis-gaditana-for-biodiesel-production
#16
Bora Kim, Yong Keun Chang, Jae W Lee
In situ transesterification of wet microalgae is a promising, simplified alternative biodiesel production process that replaces multiple operations of cell drying, extraction, and transesterification reaction. This study addresses enhanced biodiesel production from Nannochloropsis gaditana at elevated temperatures. Compared with the previously reported in situ transesterification process of conducting the reaction at a temperature ranging from 95 to 125 °C, the present work employs higher temperatures of at least 150 °C...
February 16, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28208796/recent%C3%A2-trends%C3%A2-in%C3%A2-marine%C3%A2-phycotoxins%C3%A2-from%C3%A2-australian%C3%A2-coastal%C3%A2-waters
#17
Penelope Ajani, D Tim Harwood, Shauna A Murray
Phycotoxins, which are produced by harmful microalgae and bioaccumulate in the  marine food web, are of growing concern for Australia. These harmful algae pose a threat to  ecosystem and human health, as well as constraining the progress of aquaculture, one of the fastest  growing food sectors in the world. With better monitoring, advanced analytical skills and an  increase in microalgal expertise, many phycotoxins have been identified in Australian coastal  waters in recent years...
February 9, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28207991/high-yield-secretion-of-recombinant-proteins-from-the-microalga-chlamydomonas-reinhardtii
#18
E M Ramos-Martinez, L Fimognari, Y Sakuragi
Microalga-based biomanufacturing of recombinant proteins is attracting growing attention due to its advantages in safety, metabolic diversity, scalability, and sustainability. Secretion of recombinant proteins can accelerate the use of microalgal platforms by allowing post-translational modifications and easy recovery of products from the culture media. However, currently, the yields of secreted recombinant proteins are low, which hampers the commercial application of this strategy. This study aimed at expanding the genetic tools for enhancing secretion of recombinant proteins in Chlamydomonas reinhardtii, a widely used green microalga as a model organism and a potential industrial biotechnology platform...
February 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28205600/non-reliance-of-metazoans-on-stromatolite-forming-microbial-mats-as-a-food-resource
#19
Gavin M Rishworth, Renzo Perissinotto, Matthew S Bird, Nadine A Strydom, Nasreen Peer, Nelson A F Miranda, Jacqueline L Raw
Grazing and burrowing organisms usually homogenise microalgal mats that form on benthic sediments of many aquatic ecosystems. In the absence of this disruption, microalgal mats can accrete laminated deposits (stromatolites). Stromatolites are rare in modern coastal ecosystems, but persist at locations where metazoans are largely excluded. This study aimed to assess the trophic structure at stromatolite locations where metazoans co-occur, to determine the grazing influence exerted by the metazoans on the stromatolite-forming microalgae (cyanobacteria and diatoms)...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28203229/editorial-biotechnology-of-microalgae-based-on-molecular-biology-and-biochemistry-of-eukaryotic-algae-and-cyanobacteria
#20
EDITORIAL
Takashi Osanai, Youn-Il Park, Yuki Nakamura
No abstract text is available yet for this article.
2017: Frontiers in Microbiology
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