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https://www.readbyqxmd.com/read/28534718/jasmonate-suppresses-seedling-soil-emergence-in-arabidopsis-thaliana
#1
Lulu Yao, Yuyu Zheng, Ziqiang Zhu
In addition to defense response, phytohormone jasmonate participates in various plant growth and developmental processes. Nonetheless, its role in the seedling stage is not well defined. We recently report that jasmonate suppresses hypocotyl elongation and promotes cotyledon unfolding in etiolated Arabidopsis seedlings. The molecular basis underlying this phenotype is that jasmonate treatment reduces the biochemical activity of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) to stabilize several COP1-targetted transcription factors for eliciting a proportion of light responsive transcriptome...
May 23, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28534654/functions-and-substrates-of-plastoglobule-localized-metallopeptidase-pgm48
#2
Nazmul H Bhuiyan, Klaas J van Wijk
Plastoglobules (PGs) in chloroplasts are monolayer lipid-protein particles attached to thylakoids. The size and number of PGs per chloroplast respond dynamically to abiotic environmental stresses and developmental transitions. During senescence, the thylakoid membranes and its constituents are dismantled in controlled fashion. Leaf senescence coincides with a dramatic increase in the size of PGs, which is consistent with a functional role of PG in remobilization of thylakoid membrane components. In a recent publication (1) , we showed that PG-localized metallopeptidase PGM48 promotes natural senescence...
May 23, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28534650/functions-of-iqd-proteins-as-hubs-in-cellular-calcium-and-auxin-signaling-a-toolbox-for-shape-formation-and-tissue-specification-in-plants
#3
Katharina Bürstenbinder, Dipannita Mitra, Jakob Quegwer
Ca(2+) ions play pivotal roles as second messengers in intracellular signal transduction, and coordinate many biological processes. Changes in intracellular Ca(2+) levels are perceived by Ca(2+) sensors such as CaM/CML proteins, which transduce Ca(2+) signals into cellular responses by regulation of diverse target proteins. Insights into molecular functions of CaM targets are thus essential to understand the molecular and cellular basis of Ca(2+) signaling. During the last decade, IQD proteins emerged as the largest class of CaM targets in plants with mostly unknown functions...
May 23, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28534065/tailoring-high-density-oligonucleotide-arrays-for-transcript-profiling-of-different-arabidopsis-thaliana-accessions-using-a-sequence-based-approach
#4
Anastassia Boudichevskaia, Hieu Xuan Cao, Renate Schmidt
Excluding polymorphic probes from GeneChip (®) transcript profiling experiments via a sequence-based approach results in improved detection of differentially expressed genes in developing seeds of Arabidopsis thaliana accessions Col-0 and C24. GeneChip(®) arrays represent a powerful tool for transcript profiling experiments. The ATH1 GeneChip(®) has been designed based on the sequence of the Arabidopsis thaliana reference genome Col-0, hence the features on the array exactly match the sequences of Col-0 transcripts...
May 22, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28533791/jasmonoyl-l-tryptophan-disrupts-iaa-activity-through-the-aux1-auxin-permease
#5
Paul Staswick, Martha Rowe, Edgar P Spalding, Bessie L Splitt
Amide-linked conjugates between tryptophan (Trp) and jasmonic (JA) or indole-3-acetic (IAA) acids interfered with gravitropism and other auxin-dependent activities in Arabidopsis, but the mechanism was unclear. To identify structural features necessary for activity several additional Trp conjugates were synthesized. The phenylacetic acid (PAA) conjugate was active, while several others were not. Common features of active conjugates is that they have ring structures that are linked to Trp through an acetic acid side chain, while longer or shorter linkages are inactive or less active...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28533213/a-novel-n-methyltransferase-in-arabidopsis-appears-to-feed-a-conserved-pathway-for-nicotinate-detoxification-among-land-plants-and-is-associated-with-lignin-biosynthesis
#6
Wei Li, Fengxia Zhang, Ranran Wu, Lijia Jia, Guosheng Li, Yalong Guo, Cuimin Liu, Guodong Wang
The Preiss-Handler pathway, which salvages nicotinate (NA) for NAD synthesis, is an indispensable biochemical pathway in land plants. Various NA conjugations (mainly methylation and glycosylation) have been detected, and have long been proposed for NA detoxification in plants. Previously, we demonstrated that NA O-glucosylation functions as a mobilizable storage form for NAD biosynthesis in the Brassicaceae. However, little is known about the functions of other NA conjugations in plants. In this study, we firstly found that N-methylnicotinate is a ubiquitous NA conjugation in land plants...
May 22, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28532507/isoprenoid-biosynthesis-in-dandelion-latex-is-enhanced-by-the-overexpression-of-three-key-enzymes-involved-in-the-mevalonate-pathway
#7
Katharina M Pütter, Nicole van Deenen, Kristina Unland, Dirk Prüfer, Christian Schulze Gronover
BACKGROUND: Latex from the dandelion species Taraxacum brevicorniculatum contains many high-value isoprenoid end products, e.g. triterpenes and polyisoprenes such as natural rubber. The isopentenyl pyrophosphate units required as precursors for these isoprenoids are provided by the mevalonate (MVA) pathway. The key enzyme in this pathway is 3-hydroxy-methyl-glutaryl-CoA reductase (HMGR) and its activity has been thoroughly characterized in many plant species including dandelion. However, two enzymes acting upstream of HMGR have not been characterized in dandelion latex: ATP citrate lyase (ACL), which provides the acetyl-CoA utilized in the MVA pathway, and acetoacetyl-CoA thiolase (AACT), which catalyzes the first step in the pathway to produce acetoacetyl-CoA...
May 22, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28532301/heterotrimeric-g-protein-regulatory-circuits-in-plants-conserved-and-novel-mechanisms
#8
Sona Pandey
Article Addendum Efficient activation and deactivation of Gα protein is critical for the regulation of heterotrimeric G-protein mediated signaling pathways. While the core G-protein components and their activation/deactivation chemistries are broadly conserved throughout the eukaryotic evolution, their regulatory mechanisms seem to have been rewired in plants to meet specific needs. Plants such as Arabidopsis, which have a limited number of G-protein components and their regulators, offer a unique opportunity to dissect the mechanistic details of distinct signaling pathways...
May 22, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28532230/hsp90-stabilizes-auxin-receptor-tir1-and-ensures-plasticity-of-auxin-responses
#9
Etsuko Watanabe, Shoji Mano, Ikuko Hara-Nishimura, Mikio Nishimura, Kenji Yamada
Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone that facilitates the maturation of target proteins. Here, we report that the auxin receptor TIR1 is a target of cytosolic HSP90 and that HSP90 and TIR1 form a complex. Inhibition of HSP90 compromised the nuclear localization of TIR1, and abrogated plant responses to the hormone auxin. Our findings suggest that HSP90 positively regulates auxin receptor function. We also propose that HSP90 buffers or hides phenotypic variations in animals and plants by masking mutations in some of its target proteins...
May 22, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28531348/plant-derived-transcription-factors-for-orthologous-regulation-of-gene-expression-in-the-yeast-saccharomyces-cerevisiae
#10
Gita Naseri, Salma Balazadeh, Fabian Machens, Iman Kamranfar, Katrin Messerschmidt, Bernd Mueller-Roeber
Control of gene expression by transcription factors (TFs) is central in many synthetic biology projects where tailored expression of one or multiple genes is often needed. As TFs from evolutionary distant organisms are unlikely to affect gene expression in a host of choice, they represent excellent candidates for establishing orthogonal control systems. To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a library of 106 different combinations of chromosomally integrated TFs, activation domains (yeast GAL4 AD, herpes simplex virus VP64, and plant EDLL) and synthetic promoters harbouring cognate cis-regulatory motifs driving a yEGFP reporter...
May 22, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28531314/the-solanum-tuberosum-kst1-partial-promoter-as-a-tool-for-guard-cell-expression-in-multiple-plant-species
#11
Gilor Kelly, Nitsan Lugassi, Eduard Belausov, Dalia Wolf, Belal Khamaisi, Danja Brandsma, Jayaram Kottapalli, Lena Fidel, Batsheva Ben-Zvi, Aiman Egbaria, Atiako Kwame Acheampong, Chuanlin Zheng, Etti Or, Assaf Distelfeld, Rakefet David-Schwartz, Nir Carmi, David Granot
To date, guard cell promoters have been examined in only a few species, primarily annual dicots. A partial segment of the potato (Solanum tuberosum) KST1 promoter (KST1 partial promoter, KST1ppro) has previously been shown to confer guard cell expression in potato, tomato (Solanum lycopersicum), citrus [Troyer citrange (C. sinensis×Poncirus trifoliata)], and Arabidopsis (Arabidopsis thaliana). Here, we describe an extensive analysis of the expression pattern of KST1ppro in eight (previously reported, as well as new) species from five different angiosperm families, including the Solanaceae and the Cucurbitaceae, Arabidopsis, the monocot barley (Hordeum vulgare), and two perennial species: grapevine (Vitis vinifera) and citrus...
May 22, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28531251/ca2-dependent-phosphoregulation-of-the-plasma-membrane-ca2-atpase-aca8-modulates-stimulus-induced-calcium-signatures
#12
Alex Costa, Laura Luoni, Claudia Adriana Marrano, Kenji Hashimoto, Philipp Köster, Sonia Giacometti, Maria Ida De Michelis, Jörg Kudla, Maria Cristina Bonza
Ca2+ signals are transient, hence, upon a stimulus-induced increase in cytosolic Ca2+ concentration, cells have to re-establish resting Ca2+ levels. Ca2+ extrusion is operated by a wealth of transporters, such as Ca2+ pumps and Ca2+/H+ antiporters, which often require a rise in Ca2+ concentration to be activated. Here, we report a regulatory fine-tuning mechanism of the Arabidopsis thaliana plasma membrane-localized Ca2+-ATPase isoform ACA8 that is mediated by calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) complexes...
May 20, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28531235/rna-seq-analysis-of-gene-expression-for-floral-development-in-crested-wheatgrass-agropyron-cristatum-l
#13
Fangqin Zeng, Bill Biligetu, Bruce Coulman, Michael P Schellenberg, Yong-Bi Fu
Crested wheatgrass [Agropyron cristatum L. (Gaertn.)] is widely used for early spring grazing in western Canada and the development of late maturing cultivars which maintain forage quality for a longer period is desired. However, it is difficult to manipulate the timing of floral transition, as little is known about molecular mechanism of plant maturity in this species. In this study, RNA-Seq and differential gene expression analysis were performed to investigate gene expression for floral initiation and development in crested wheatgrass...
2017: PloS One
https://www.readbyqxmd.com/read/28530801/hydrogen-bonding-environment-of-the-n3-h-group-of-fmn-in-the-lov-domains-of-phototropins
#14
Tatsuya Iwata, Dai Nozaki, Atsushi Yamamoto, Takayuki Koyama, Yasuzo Nishina, Kiyoshi Shiga, Satoru Tokutomi, Masashi Unno, Hideki Kandori
LOV (light-oxygen-voltage) domain is a flavin-binding blue-light receptor domain originally found in plant phototropin, a photoreceptor for phototropism, chloroplast movement and stomata opening. LOV domains are available as optogenetics tools for photosensory domain of fusion proteins. Therefore, it is important to investigate how LOV domains show light-induced structural changes not only for the elucidation of regulation mechanism of kinase domain but also for the appropriate activation of other functional domains by LOV domain...
May 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28529882/transcriptomic-data-of-arabidopsis-thaliana-hypocotyl-upon-suppression-of-expansin-genes
#15
Iqmal Asyraf Ilias, Othman Babul Airianah, Syarul Nataqain Baharum, Hoe-Han Goh
Expansin is a cell wall loosening protein without hydrolytic activity, which allows cell expansion by influencing cell wall extensibility. Previous studies showed that the suppression of expansin genes (EXPA1, EXPA3, EXPA5 and EXPA10) resulted in defective organ growth and altered cell wall chemical composition [1,2]. However, the molecular mechanism on how the suppression of non-enzymatic expansin expression can result in widespread effects on plant cell wall and organ growth is still unclear. In this study, we performed transcriptomic analysis on the hypocotyls of previously reported transgenic Arabidopsis line [1] to investigate the effects of expansin gene suppression on the global gene expression pattern, particularly on the cell wall related genes...
June 2017: Genomics Data
https://www.readbyqxmd.com/read/28529706/expresso-a-database-and-web-server-for-exploring-the-interaction-of-transcription-factors-and-their-target-genes-in-arabidopsis-thaliana-using-chip-seq-peak-data
#16
Delasa Aghamirzaie, Karthik Raja Velmurugan, Shuchi Wu, Doaa Altarawy, Lenwood S Heath, Ruth Grene
Motivation: The increasing availability of chromatin immunoprecipitation sequencing (ChIP-Seq) data enables us to learn more about the action of transcription factors in the regulation of gene expression. Even though in vivo transcriptional regulation often involves the concerted action of more than one transcription factor, the format of each individual ChIP-Seq dataset usually represents the action of a single transcription factor. Therefore, a relational database in which available ChIP-Seq datasets are curated is essential...
2017: F1000Research
https://www.readbyqxmd.com/read/28529520/katanin-1-is-essential-for-embryogenesis-and-seed-formation-in-arabidopsis
#17
Ivan Luptovčiak, Despina Samakovli, George Komis, Jozef Šamaj
Cytoskeletal remodeling has a fundamental role, especially during transitional developmental stages when cells rapidly adopt new forms and roles, like gametogenesis, fertilization and concomitant embryogenesis and seed formation. KATANIN 1, a microtubule severing protein, fulfills a major regulatory mechanism of dynamic microtubule turnover in eukaryotes. Herein, we show that three well-established KATANIN 1 mutants, fra2, lue1 and ktn1-2 collectively display lower fertility and seed set in Arabidopsis. These lower fertility and seed set rates of fra2, lue1 and ktn1-2 mutants were correlated to abnormalities in the development of embryo proper and seed...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28529501/microbial-degradation-of-lobster-shells-to-extract-chitin-derivatives-for-plant-disease-management
#18
Gayathri Ilangumaran, Glenn Stratton, Sridhar Ravichandran, Pushp S Shukla, Philippe Potin, Samuel Asiedu, Balakrishnan Prithiviraj
Biodegradation of lobster shells by chitinolytic microorganisms are an environment safe approach to utilize lobster processing wastes for chitin derivation. In this study, we report degradation activities of two microbes, "S223" and "S224" isolated from soil samples that had the highest rate of deproteinization, demineralization and chitinolysis among ten microorganisms screened. Isolates S223 and S224 had 27.3 and 103.8 protease units mg(-1) protein and 12.3 and 11.2 μg ml(-1) of calcium in their samples, respectively, after 1 week of incubation with raw lobster shells...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28527975/the-reaumuria-trigyna-transcription-factor-rtwrky1-confers-tolerance-to-salt-stress-in-transgenic-arabidopsis
#19
Chao Du, Pingping Zhao, Huirong Zhang, Ningning Li, Linlin Zheng, Yingchun Wang
Reaumuria trigyna (R. trigyna) is an endangered small shrub endemic to the Eastern Alxa-Western Ordos area in Inner Mongolia, China. Based on R. trigyna transcriptome data, the Group I WRKY transcription factor gene RtWRKY1 was cloned from R. trigyna. The full-length RtWRKY1 gene was 2100bp, including a 1261-bp open reading frame (ORF) encoding 573 amino acids. RtWRKY1 was mainly expressed in the stem and was induced by salt, cold stress, and ABA treatment. Overexpression of RtWRKY1 in Arabidopsis significantly enhanced the chlorophyll content, root length, and fresh weight of the transgenic lines under salt stress...
May 4, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28527971/identification-and-comprehensive-evaluation-of-reference-genes-for-rt-qpcr-analysis-of-host-gene-expression-in-brassica-juncea-aphid-interaction-using-microarray-data
#20
Chet Ram, Murali Krishna Koramutla, Ramcharan Bhattacharya
Brassica juncea is a chief oil yielding crop in many parts of the world including India. With advancement of molecular techniques, RT-qPCR based study of gene-expression has become an integral part of experimentations in crop breeding. In RT-qPCR, use of appropriate reference gene(s) is pivotal. The virtue of the reference genes, being constant in expression throughout the experimental treatments, needs to be validated case by case. Appropriate reference gene(s) for normalization of gene-expression data in B...
May 11, 2017: Plant Physiology and Biochemistry: PPB
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