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Ana Carolina Prado Sousa, Carlo José Freire Oliveira, Matias Pablo Juan Szabó, Marcelo José Barbosa Silva
Cancer is one of the most troubling diseases and is becoming increasingly common. Breast cancer has a high cure rate when diagnosed early, but when diagnosed late, treatment is frequently painful, devastating and unsuccessful. The search for new treatments that are more effective and less harmful has led to several substances and biomolecules from plants and animals with potential anti-tumor activity. Within this context, ticks have emerged as an excellent source of new molecules with a wide array of therapeutic properties...
April 26, 2018: Toxicon: Official Journal of the International Society on Toxinology
José Ribamar Lima de Souza, Rafael Neodini Remedio, André Arnosti, Rusleyd Maria Magalhães de Abreu, Maria Izabel Camargo-Mathias
Several studies searching for methods to control Rhipicephalus sanguineus s.l., (dog tick) infestations have been developed aiming to minimize the damages caused by these ectoparasites to the hosts and the environment, which is harmed by the indiscriminate use of toxic acaricide products. In this scenario, neem oil has been used as a natural alternative against ticks, once this chemical has repellent properties and interferes in the growth regulation of these ectoparasites, inhibiting ecdysis. The present study evaluated the effects of azadirachtin-enriched neem oil on the integument of semi-engorged R...
August 2017: Microscopy Research and Technique
C M Krishna Murthy, K J Ananda, J Adeppa
A study was carried out to ascertain the status of ecto-parasites infesting dogs of Shimoga region. A total of 120 dogs with the history of skin problems presented to the local hospitals and local pet clinics of Shimoga region were screened for different ecto-parasites. The ticks, fleas and lice were collected, processed and identified. The skin scrapings were also collected from the affected dogs and processed for identification of mites. Out of 120 dogs examined, 59 (49.1 %) had harboured ecto-parasites...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
J Petermann, L Cauquil, J C Hurlin, H Gaia, T Hüe
The evolution of tick resistance to acaricides in New Caledonia was followed in two ways: through two large scale surveys in 1998 and 2014 and through the routine analysis of samples of ticks with suspected resistance. The results of the two approaches were qualitatively similar though analysis of ticks with suspected resistance gave higher frequencies of resistance, as expected of a biased sample. Resistance tests using a larval packet test have been conducted since 1993 for deltamethrin and 2003 for amitraz...
February 15, 2016: Veterinary Parasitology
Ana Hurtado, Jesús F Barandika, Beatriz Oporto, Esmeralda Minguijón, Inés Povedano, Ana L García-Pérez
This study was designed to investigate an outbreak of high mortality that occurred in naïve Assaf sheep introduced into a Latxa sheep flock in the Basque Country, a region where piroplasmosis is endemic. To identify the causes of this outbreak, a panel of different methods, including traditional pathological, biopathological and parasitological analyses combined with recently developed molecular methods, was used. These novel molecular methods included a multiplex real-time PCR assay to screen for the presence of the most important tick-borne pathogens (piroplasms and anaplasmas), followed by a second species-specific multiplex real-time PCR assay for the identification of Anaplasma-positive samples...
February 2015: Ticks and Tick-borne Diseases
Eve Miguel, Thierry Boulinier, Michel de Garine-Wichatitsky, Alexandre Caron, Hervé Fritz, Vladimir Grosbois
The sharing of habitat by wild and domestic animals may result in pathogen transmission, notably via ectoparasite vectors such as ticks. Interfaces between protected and communal lands constitute sharp transitions between areas occupied by host communities that are extremely contrasted in terms of composition, diversity and density. Empirical characterizations of tick communities and of their vertebrate hosts are strongly relevant for understanding the mechanisms leading to disease transmission between wild and domestic animals...
October 2014: Acta Tropica
Hermes Ribeiro Luz, João Luiz Horacio Faccini, Gabriel Alves Landulfo, Bruno Pereira Berto, Ildemar Ferreira
In the present study the tick prevalence, mean intensity of infestation and species were recorded on birds captured between January 2009 and December 2010 in the Ecological Station Pirapitinga-ESEC from Minas Gerais State, Brazil. A total of 967 birds, from 15 families and 40 species were captured and 165 (17.1 %) individuals were parasitized by ticks. Of these 160 (97 %) belonged to the order Passeriformes. Five tick species were identified: Amblyomma longirostre (n = 274) was the most common species followed by Amblyomma parvum (n = 43), Amblyomma nodosum (n = 39), Amblyomma ovale (n = 24) and Riphicephalus sanguineus (n = 7)...
September 2012: Experimental & Applied Acarology
R Corrain, M Drigo, M Fenati, M L Menandro, A Mondin, D Pasotto, M Martini
A survey on tick density and on tick-borne zoonoses was carried out in four public parks in the outskirts of Imola (northern Italy) from June to October 2006. All stages of Ixodes ricinus and only larvae of Riphicephalus sanguineus were recovered by dragging, performed on 100-m transects. Almost all ticks (99%) were harvested in one park. I. ricinus density (nymphs/100 m(2) ) ranged from 0 in park L to 6.3 in park F. Nymphs and adults of I. ricinus were subjected to PCR for Anaplasma phagocytophilum, Bartonella spp...
November 2012: Zoonoses and Public Health
G Aguilar-Tipacamú, R Rosario-Cruz, Robert J Miller, Felix D Guerrero, R I Rodriguez-Vivas, Z García-Vázquez
Dialelic crosses and backcrosses of pyrethroid resistant (RR) and susceptible (SS) Rhipicephalus (Boophilus) microplus tick strains were carried out and the substitution (Phe-Ile) within the sodium channel gene was monitored in order to analyze the effects of the genotype on the pyrethroid resistance phenotype as measured by the larval packet test (LPT). Parental strains: susceptible (SS) and resistant (RR); dialelic crosses: RS (♂RR × ♀SS), and SR (♂SS × ♀RR); and backcrosses: RS × SS, RS × RR, SR × SS and SR × RR were infested on 280 kg calves...
July 2011: Experimental & Applied Acarology
Vera Lucia Sardá Ribeiro, Jaqueline Campiol Dos Santos, Sérgio A L Bordignon, Miriam A Apel, Amélia T Henriques, Gilsane L von Poser
Hesperozygis ringens (Benth.) Epling (Lamiaceae) is a strongly aromatic plant employed popularly for its antiparasitic properties. The leaves afforded 4% of essential oil constituted mainly by pulegone (86%). Laboratory tests were carried out to determine the toxicity of the essential oil species on engorged females and larvae of the cattle tick Riphicephalus (Boophilus) microplus using the adult immersion test (AIT) and the larval immersion test (LIT). It was observed that the essential oil at the concentration of 50 microL/mL and 25 microL/mL inhibited the egg laying significatively in relation to the controls and the eggs from these treated females were affected by the oil; the hatching was inhibited in 95% and 30%, respectively...
April 2010: Bioresource Technology
Miriam A Apel, Vera Lucia Sardá Ribeiro, Sérgio A L Bordignon, Amélia T Henriques, Gilsane von Poser
The essential oils obtained from the aerial parts of five species of Cunila (Lamiaceae) native to Southern Brazil were analyzed by GC and GC/MS. The oil of Cunila angustifolia was characterized by sabinene; Cunila incana is rich in alpha-pinene and beta-pinene, Cunila spicata and Cunila microcephala presented menthofuran as the main component, and in the essential oil of Cunila incisa, the major component was 1,8-cineole. Laboratory tests were carried out to determine the effect of the essential oils of the above cited plants on larvae of the cattle tick Riphicephalus (Boophilus) microplus...
September 2009: Parasitology Research
Antonio P de Souza, Luana Paula H N Veiga, Valdomiro Bellato, Amélia Aparecida Sartor, Cristina P Cardoso, Ana Paula de O Nunes
The aim of this work was to develop a technique of acaricides evaluation by immersion of larvae. Larvae were obtained from 30 engorged females of Riphicephalus (Boophilus) microplus were collected in a ranch in Lages, Santa Catarina state, Brazil. Larvae were obtained from 10mg of eggs placed into 5ml disposable syringes, previously prepared and kept under controlled conditions of laboratory, and collected between the third and the twelfth day of oviposition. Seven to ten days old larvae were submitted to the acaricide laboratory test, in ten replications, by their immersion in eight dilutions of commercial acaricides (amitraz or cypermethrin), for 30 seconds; for each replication and commercial product, a control group was immersed in distilled water...
October 2008: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
Paula C Pohl, Marcos Henrique F Sorgine, Alexandre T Leal, Carlos Logullo, Pedro L Oliveira, Itabajara da Silva Vaz, Aoi Masuda
An aspartic endopeptidase named THAP, from the eggs of the tick Riphicephalus (Boophilus) microplus, has been suggested to be involved in vitellin-degradation. Here we characterized this enzyme further, showing that THAP mRNA is present in the fat body, midgut and ovary of ticks, in two developmental stages (partially and fully engorged females). However, higher transcription levels were found in fully engorged vitellogenic females. The THAP protein was detected in the haemolymph, midgut and fat body and, in higher quantity, in the ovary of fully engorged females, and it was present throughout embryo development...
December 2008: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
K A Oliveira, L S Oliveira, C C A Dias, A Silva, M R Almeida, G Almada, D H Bouyer, M A M Galvão, Cl Mafra
Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin...
March 2008: Memórias do Instituto Oswaldo Cruz
G Gasparin, M Miyata, L L Coutinho, M L Martinez, R L Teodoro, J Furlong, M A Machado, M V G B Silva, T S Sonstegard, L C A Regitano
Differences in domestication and selection processes have contributed to considerable phenotypic and genotypic differences between Bos taurus and Bos indicus cattle breeds. Of particular interest in tropical and subtropical production environments are those genetic differences between subspecies that underlie the phenotypic extremes in tolerance and susceptibility to parasite infection. In general, B. taurus cattle are more susceptible to ectoparasites than B. indicus cattle in tropical environments, and much of this difference is under genetic control...
October 2007: Animal Genetics
Kaushal Kumar, N Balakrishanan, Rakesh Katyal, Kuldip Singh Gill
The Nilgiri hills provides favourable ecological conditions for the propagation of haematophagous arthropods due to its richness in vegetation and animal fauna. A study was undertaken by the NICD during August to November 1996 on the prevalence of ectoparasitic ticks from different localities of the Nilgiris district of Tamil Nadu state. The ticks were hand picked from various domestic animals of the study area and identified. A total of 1232 adults and immatures of ticks were collected from domestic animals which comprised of the various species in the order of abundance Boophilus microplus, Haemaphysalis bispinosa, Riphicephalus haemaphysaloides and Riphicephalus sanguineus...
June 2002: Journal of Communicable Diseases
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