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https://www.readbyqxmd.com/read/27909300/cyanobacterial-photoprotection-by-the-orange-carotenoid-protein
#1
REVIEW
Diana Kirilovsky, Cheryl A Kerfeld
In photosynthetic organisms, the production of dangerous oxygen species is stimulated under high irradiance. To cope with this stress, these organisms have evolved photoprotective mechanisms. One type of mechanism functions to decrease the energy arriving at the photochemical centres by increasing thermal dissipation at the level of antennae. In cyanobacteria, the trigger for this mechanism is the photoactivation of a soluble carotenoid protein, the orange carotenoid protein (OCP), which is a structurally and functionally modular protein...
December 2, 2016: Nature Plants
https://www.readbyqxmd.com/read/27907029/long-term-stable-and-tightly-controlled-expression-of-recombinant-proteins-in-antibiotics-free-conditions
#2
Soo-Jin Yeom, Dae-Hee Lee, Yu Jung Kim, Jeongmin Lee, Kil Koang Kwon, Gui Hwan Han, Haseong Kim, Hak-Sung Kim, Seung-Goo Lee
Plasmid-based gene expression is a fundamental tool in the field of biotechnology. However, overexpression of genes of interest with multi-copy plasmids often causes detrimental effects on host cells. To overcome this problem, chromosomal integration of target genes has been used for decades; however, insufficient protein expression occurred with this method. In this study, we developed a novel cloning and expression system named the chromosomal vector (ChroV) system, that has features of stable and high expression of target genes on the F' plasmid in the Escherichia coli JM109(DE3) strain...
2016: PloS One
https://www.readbyqxmd.com/read/27902319/description-of-gramella-forsetii-sp-nov-a-marine-flavobacteriaceae-isolated-from-north-sea-water-and-emended-description-of-gramella-gaetbulicola-cho-et-al-2011
#3
Irina Panschin, Mareike Becher, Susanne Verbarg, Cathrin Spröer, Manfred Rohde, Marga Schüler, Rudolf I Amann, Jens Harder, Brian J Tindall, Richard L Hahnke
The strain KT0803T was isolated from coastal eutrophic surface waters of Helgoland Roads near the island of Helgoland, North Sea, Germany. The taxonomic position of the strain, previously known as 'Gramella forsetii' KT0803, was investigated by a polyphasic approach. The strain was Gram-stain negative, chemo-organotrophic, heterotrophic, strictly aerobic, oxidase and catalase positive, rod-shaped, motile by gliding and had orange-yellow carotenoid pigments, but was negative for flexirubin-type pigments. It grew optimally at 22-25 °C, pH 7...
November 28, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902289/polymorphobacter-glacialis-sp-nov-isolated-from-ice-core%C3%AF-china
#4
Tingting Xing, Yongqin Liu, Baiqing Xu, Ninglian Wang, Keshao Liu, Liang Shen, Zhengquan Gu, Bixi Guo, Hongcan Liu, Yuguang Zhou
A Gram-stain-negative, non-spore-forming, rod-shaped bacterium, B555-2T was isolated from an ice core drilled from Muztagh Glacier on the Tibetan Plateau, China. According to phylogenetic analysis with 16S rRNA ribosomal gene sequences, the novel strain was most closely related to Polymorphobacter fuscus D40PT and Polymorphobacter multimanifer 262-7T with 98.4% and 96.9% similarity, respectively. It grew optimally at pH 7 and 15 °C with 0.6% NaCl (m/v). Carotenoid was detected from the cells. Major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid, phophatidylmonomethy lethanolamine, phophatidulcholine, unidentified phospholipid and 5 unidentified lipids...
November 16, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27901343/colonization-of-snow-by-microorganisms-as-revealed-using-miniature-raman-spectrometers-possibilities-for-detecting-carotenoids-of-psychrophiles-on-mars
#5
Jan Jehlička, Adam Culka, Linda Nedbalová
We tested the potential of a miniaturized Raman spectrometer for use in field detection of snow algae pigments. A miniature Raman spectrometer, equipped with an excitation laser at 532 nm, allowed for the detection of carotenoids in cells of Chloromonas nivalis and Chlamydomonas nivalis at different stages of their life cycle. Astaxanthin, the major photoprotective pigment, was detected in algal blooms originating in snows at two alpine European sites that differed in altitude (Krkonoše Mts., Czech Republic, 1502 m a...
November 30, 2016: Astrobiology
https://www.readbyqxmd.com/read/27899047/phytochemicals-and-antioxidant-capacity-in-four-italian-traditional-maize-zea-mays-l-varieties
#6
Antonella Capocchi, Stefania Bottega, Carmelina Spanò, Debora Fontanini
Flours of four pigmented (from orange to red and dark red) local Italian corns, studied for their soluble, soluble conjugate, and insoluble-bound phenols and flavonoids, showed a prevalence of the insoluble-bound fraction (70-80%). Correlations were found between the flours antioxidant capacity, measured with CUPRAC, FRAP, and DPPH methods, and soluble phenols and flavonoids content. A correlation was also found between ascorbic acid content and flours antioxidant power. Anthocyanins were present in small amounts in the red/dark red seeds; however, acid-alcohol assays and spectral analyses of pericarp extracts indicated the presence of red-brick phlobaphenes in these varieties...
November 29, 2016: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/27898731/reproducibility-and-validity-of-a-food-frequency-questionnaire-designed-to-assess-diet-in-children-aged-4-5-years
#7
Jesus Vioque, Daniel Gimenez-Monzo, Eva Maria Navarrete-Muñoz, Manuela Garcia-de-la-Hera, Sandra Gonzalez-Palacios, Marisa Rebagliato, Ferran Ballester, Mario Murcia, Carmen Iñiguez, Fernando Granado
BACKGROUND: The food frequency questionnaire (FFQ) is the most efficient and cost-effective method to investigate the relationship between usual diet and disease in epidemiologic studies. Although FFQs have been validated in many adult populations worldwide, the number of valid FFQ in preschool children is very scarce. The aim of this study was to evaluate the reproducibility and validity of a semi-quantitative FFQ designed for children aged 4 to 5 years. MATERIALS AND METHODS: In this study, we have included 169 children aged 4-5 years from the INMA project in Valencia, a population-based prospective cohort study of mothers and children in Spain...
2016: PloS One
https://www.readbyqxmd.com/read/27895288/some-biological-activities-of-pigments-extracted-from-micrococcus-roseus-ptcc-1411-and-rhodotorula-glutinis-ptcc-5257
#8
Hossein Rostami, Hassan Hamedi, Mahmoud Yolmeh
The importance of replacing synthetic pigments with natural types is increasing day by day in the food industry due to the harmful effects of some synthetic pigments. Microorganisms are a major source of natural pigments, which nowadays have attracted the attention of researchers. In this study, carotenoid pigments were produced by Micrococcus roseus and Rhodotorula glutinis, and some of their biological properties such as antimicrobial, antioxidant, anticancer, and anti-inflammatory activities were evaluated...
December 2016: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/27895226/the-plastoglobule-localized-metallopeptidase-pgm48-is-a-positive-regulator-of-senescence-in-arabidopsis-thaliana
#9
Nazmul H Bhuiyan, Giulia Friso, Elden Rowland, Kristina Majsec, Klaas Jan van Wijk
Plastoglobuli (PG) are thylakoid-associated monolayer lipid particles with a specific proteome of ~30 PG core proteins and isoprenoid- and neutral lipids. During senescence, PG increase in size, reflecting their role in dismantling thylakoid membranes. Here we show that the only PG-localized peptidase, PGM48, positively regulates leaf senescence. We discovered that PGM48 is a member of the M48 peptidase family with PGM48 homologs, forming a clade (M48D) only found in photosynthetic organisms. Unlike the M48A, B, and C clades, members of M48D have no transmembrane domains, consistent with their unique subcellular location in the PG...
November 28, 2016: Plant Cell
https://www.readbyqxmd.com/read/27891544/an-in-vitro-digestion-method-adapted-for-carotenoids-and-carotenoid-esters-moving-forward-towards-standardization
#10
Daniele Bobrowski Rodrigues, Lilian Regina Barros Mariutti, Adriana Zerlotti Mercadante
In vitro digestion methods are a useful approach to predict the bioaccessibility of food components and overcome some limitations or disadvantages associated with in vivo methodologies. Recently, the INFOGEST network published a static method of in vitro digestion with a proposal for assay standardization. The INFOGEST method is not specific for any food component; therefore, we aimed to adapt this method to assess the in vitro bioaccessibility of carotenoids and carotenoid esters in a model fruit (Byrsonima crassifolia)...
November 28, 2016: Food & Function
https://www.readbyqxmd.com/read/27888475/assessing-contamination-of-microalgal-astaxanthin-producer-haematococcus-cultures-with-high-resolution-melting-curve-analysis
#11
Adam Dawidziuk, Delfina Popiel, Magda Luboinska, Michal Grzebyk, Maciej Wisniewski, Grzegorz Koczyk
Due to its superior antioxidant capabilities and higher activity than other carotenoids, astaxanthin is used widely in the nutraceutical and medicine industries. The most prolific natural producer of astaxanthin is the unicellular green microalga Haematococcus pluvialis. The correct identification of any contaminants in H. pluvialis cultures is both essential and nontrivial for several reasons. Firstly, while it is possible to distinguish the main microalgal contaminant Coelastrella sp. (in H. pluvialis cultures), in practice, it is frequently a daunting and error-prone task for personnel without extensive experience in the microscopic identification of algal species...
November 26, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27888323/the-calmodulin-gene-in-neurospora-crassa-is-required-for-normal-vegetative-growth-ultraviolet-survival-and-sexual-development
#12
Vijya Laxmi, Ranjan Tamuli
We isolated a Neurospora crassa mutant of the calmodulin (cmd) gene using repeat-induced point mutation and studied its phenotypes. The cmd (RIP) mutant showed a defect in growth, reduced aerial hyphae, decreased carotenoid accumulation, a severe reduction in viability upon ultraviolet (UV) irradiation, and a fertility defect. Moreover, meiotic silencing of the cmd gene resulted in a barren phenotype. In addition, we also performed site-directed mutational analysis of the calcium/calmodulin-dependent kinase-2 (Ca(2+)/CaMK-2), a target of the CaM protein encoded by the cmd gene...
November 25, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27885783/protective-effects-of-a-novel-nutritional-and-phytonutrient-blend-on-ultraviolet-radiation-induced-skin-damage-and-inflammatory-response-through-aging-defense-mechanisms
#13
Steven M Wood, Angela F Mastaloudis, Shelly N Hester, Russell Gray, Dale Kern, Jin Namkoong, Zoe D Draelos
BACKGROUND: The human body relies on several aging defense mechanisms (ADMs) to limit damage induced from pro-aging stressors (aging aggressors). However, such protective mechanisms can be compromised, leading to accelerated aging. The skin provides a model to probe the effects of an oral nutritional intervention on ADMs in response to ultraviolet radiation (UVR)-induced damage. OBJECTIVE: To determine whether supplementation with a novel nutritional and phytonutrient blend could protect against UVR-induced skin damage and positively influence facial skin attributes and characteristics by bolstering ADMs...
November 24, 2016: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/27885677/chemical-composition-of-microalgae-heterochlorella-luteoviridis-and-dunaliella-tertiolecta-with-emphasis-on-carotenoids
#14
Andressa Bacalau Diprat, Tania Menegol, Juliana Ferreira Boelter, Ariane Zmozinski, Maria Goreti Rodrigues Vale, Eliseu Rodrigues, Rosane Rech
BACKGROUND: Microalgae have been used as food supplements due their high protein, polyunsaturated fatty acids and carotenoid contents. As different carotenoids have distinct properties, and carotenoid composition from microalgae were poorly explored in the literature, this study determined the complete carotenoid composition from two microalgae species, Heterochlorella luteoviridis and Dunaliella tertiolecta, using high-performance liquid chromatography coupled to diode array detector and a mass spectrometry (HPLC-DAD-MS(2) )...
November 24, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27884594/surface-properties-of-polyene-glycol-phospholipid-monolayers
#15
Tibor Hianik, Christer L Øpstad, Jana Šandorová, Zuzana Garaiová, Vassilia Partali, Hans-Richard Sliwka
We studied the surface properties of monolayers composed of polyunsaturated conjugated ethylene glycol phospholipids (carotenoid lipids), compared the data with monolayers of dipalmitoylphosphatidylcholine (DPPC) to which carotenoids were added and evaluated the impact of the unsaturated glycol lipids on monolayers with the glycerolipid DPPC. The carotenoid based glycol lipids formed monolayers at the air/water interface. Using the Langmuir method we obtained series of pressure-area (π-A) isotherms and determined the limiting area A per molecule of three glycol lipids, C30:9-C0A=42...
November 21, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27882483/effect-of-co2-concentration-on-growth-and-biochemical-composition-of-newly-isolated-indigenous-microalga-scenedesmus-bajacalifornicus-bbklp-07
#16
Lakkanagouda Patil, Basappa Kaliwal
Photosynthetic mitigation of CO2 through microalgae is gaining great importance due to its higher photosynthetic ability compared to plants, and the biomass can be commercially exploited for various applications. CO2 fixation capability of the newly isolated freshwater microalgae Scenedesmus bajacalifornicus BBKLP-07 was investigated using a 1-l photobioreactor. The cultivation was carried at varying concentration of CO2 ranging from 5 to 25%, and the temperature and light intensities were kept constant. A maximum CO2 fixation rate was observed at 15% CO2 concentration...
November 23, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27879659/innovative-alternative-technologies-to-extract-carotenoids-from-microalgae-and-seaweeds
#17
REVIEW
Mahesha M Poojary, Francisco J Barba, Bahar Aliakbarian, Francesco Donsì, Gianpiero Pataro, Daniel A Dias, Pablo Juliano
Marine microalgae and seaweeds (microalgae) represent a sustainable source of various bioactive natural carotenoids, including β-carotene, lutein, astaxanthin, zeaxanthin, violaxanthin and fucoxanthin. Recently, the large-scale production of carotenoids from algal sources has gained significant interest with respect to commercial and industrial applications for health, nutrition, and cosmetic applications. Although conventional processing technologies, based on solvent extraction, offer a simple approach to isolating carotenoids, they suffer several, inherent limitations, including low efficiency (extraction yield), selectivity (purity), high solvent consumption, and long treatment times, which have led to advancements in the search for innovative extraction technologies...
November 22, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27877091/conserved-chemosensory-proteins-in-the-proboscis-and-eyes-of-lepidoptera
#18
Jiao Zhu, Immacolata Iovinella, Francesca Romana Dani, Yu-Ling Liu, Ling-Qiao Huang, Yang Liu, Chen-Zhu Wang, Paolo Pelosi, Guirong Wang
Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are endowed with several different functions besides being carriers for pheromones and odorants. Based on a previous report of a CSP acting as surfactant in the proboscis of the moth Helicoverpa armigera, we revealed the presence of orthologue proteins in two other moths Plutella xylostella and Chilo suppressalis, as well as two butterflies Papilio machaon and Pieris rapae, using immunodetection and proteomic analysis. The unusual conservation of these proteins across large phylogenetic distances indicated a common specific function for these CSPs...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27872932/phytochemical-profiles-of-marine-phytoplanktons-an-evaluation-of-their-in-vitro-antioxidant-and-anti-proliferative-activities
#19
Jim Jun-Hui Huang, Wen-Wen Xu, Shao-Ling Lin, Peter Chi-Keung Cheung
Marine microorganisms such as phytoplanktons are a rich resource of bioactive components with antioxidant and anti-proliferative activities that can act as novel functional food ingredients. In this study, the pigment profiles, total mycosporine-like amino acids (MAAs) and total phenolic contents (TPCs) in solvent extracts including 90% acetone and methanol from five marine phytoplanktons including Nitzschia closterium (Bacillariophyta), Isochrysis zhangjiangensis (Haptophyta), Platymonas subcordiformis (Chlorophyta), Porphyridium cruentum (Rhodophyta) and Synechocystis pevalekii (Cyanobacteria) were analyzed...
November 22, 2016: Food & Function
https://www.readbyqxmd.com/read/27872240/the-staphylococcus-aureus-airsr-two-component-system-mediates-reactive-oxygen-species-resistance-via-transcriptional-regulation-of-staphyloxanthin-production
#20
Jeffrey W Hall, Junshu Yang, Haiyong Guo, Yinduo Ji
Staphylococcus aureus is an important opportunistic pathogen and etiologically agent of many hospital- and community-acquired infections. The golden pigment, staphyloxanthin, of S. aureus colonies distinguishes it from other staphylococci and related Gram-positive cocci. Staphyloxanthin is the product of a series of biosynthetic steps that produce a unique membrane-embedded C (30) golden carotenoid and is an important antioxidant. We observed a strain with an inducible airR overexpression cassette had noticeably increased staphyloxanthin production compared to the wild-type strain under aerobic culturing conditions...
November 21, 2016: Infection and Immunity
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