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https://www.readbyqxmd.com/read/28938494/supercritical-fluid-chromatography-in-natural-product-analysis-an-update
#1
Nora Gibitz Eisath, Sonja Sturm, Hermann Stuppner
The wide chemical diversity of natural products has challenged analysts all over the world and has been a driving force for the development of innovative technologies since decades. In the last years, supercritical fluid chromatography (SFC) has finally emerged from the shadow of liquid chromatography (LC) and gas chromatography (GC) and has become a powerful tool in modern natural product analysis. Whereas in the past the technique had mainly been restricted to a small group of nonpolar compounds, it has largely expanded its suitability in the last years and has demonstrated possibilities without boundaries...
September 22, 2017: Planta Medica
https://www.readbyqxmd.com/read/28938176/genotypic-differences-in-physiological-and-biochemical-responses-to-salinity-stress-in-melon-cucumis-melo-l-plants-prospects-for-selection-of-salt-tolerant-landraces
#2
Behrooz Sarabi, Sahebali Bolandnazar, Nasser Ghaderi, Jaleh Ghashghaie
Melon (Cucumis melo L.) is one of the most important horticultural crops in Iran often cultivated in arid and semiarid regions of the country with salinity problems. The objective of this work was to better understand the mechanisms of physiological and biochemical responses to salinity stress of five Iranian melon landraces "Samsuri", "Kashan", "Khatouni", "Suski-e-Sabz", and "Ghobadlu" from different geographical origins, and "Galia" F1 cultivar. Plants were grown under greenhouse conditions and irrigated with half-strength Hoagland solution containing 0, 30, 60, or 90 mM NaCl for 60 days...
September 13, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28937591/metabolic-engineering-of-escherichia-coli-for-producing-astaxanthin-as-the-predominant-carotenoid
#3
Qian Lu, Yi-Fan Bu, Jian-Zhong Liu
Astaxanthin is a carotenoid of significant commercial value due to its superior antioxidant potential and wide applications in the aquaculture, food, cosmetic and pharmaceutical industries. A higher ratio of astaxanthin to the total carotenoids is required for efficient astaxanthin production. β-Carotene ketolase and hydroxylase play important roles in astaxanthin production. We first compared the conversion efficiency to astaxanthin in several β-carotene ketolases from Brevundimonas sp. SD212, Sphingomonas sp...
September 22, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28936877/botryococcus-as-an-alternative-source-of-carotenoids-and-its-possible-applications-an-overview
#4
Ranga Rao Ambati, Deepika Gogisetty, Ravishankar Aswathnarayana Gokare, Sarada Ravi, Panduranga Narasimharao Bikkina, Yuepeng Su, Bo Lei
The enhanced interest in carotenoid research arises partly because of their application in the food and health industries and partly because of the necessity to find a commercially viable natural source for their mass production. The bottlenecks in finding a natural source of carotenoids which can compete with the synthetic products is the mass production of the organism that produces carotenoids, cell harvesting and extraction methods of carotenoids. The microalga Botryococcus braunii is an interesting organism for its commercial value as a rich source of carotenoids...
September 22, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28936220/de-novo-assembly-of-the-camellia-nitidissima-transcriptome-reveals-key-genes-of-flower-pigment-biosynthesis
#5
Xingwen Zhou, Jiyuan Li, Yulin Zhu, Sui Ni, Jinling Chen, Xiaojuan Feng, Yunfeng Zhang, Shuangquan Li, Hongguang Zhu, Yuanguang Wen
The golden camellia, Camellia nitidissima Chi., is a well-known ornamental plant that is known as "the queen of camellias" because of its golden yellow flowers. The principal pigments in the flowers are carotenoids and flavonol glycosides. Understanding the biosynthesis of the golden color and its regulation is important in camellia breeding. To obtain a comprehensive understanding of flower development in C. nitidissima, a number of cDNA libraries were independently constructed during flower development. Using the Illumina Hiseq2500 platform, approximately 71...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28935842/the-paralogs-to-the-c-terminal-domain-of-the-cyanobacterial-ocp-are-carotenoid-donors-to-hcps
#6
Fernando Muzzopappa, Adjélé Wilson, Vinosa Yogarajah, Sandrine Cot, François Perreau, Cédric Montigny, Céline Bourcier de Carbon, Diana Kirilovsky
The photoactive Orange Carotenoid Protein photoprotects cyanobacteria cells by quenching singlet oxygen and excess excitation energy. Its N-terminal domain (NTD) is the active part of the protein and the C-terminal domain (CTD) regulates the activity. Recently, the characteristics of a family of soluble carotenoid-binding proteins (Helical Carotenoid Proteins or HCPs), paralogs of NTD-OCP, were described. Bioinformatics studies also revealed the existence of genes coding for homologs of CTD. Here, we show that the latter genes encode carotenoid proteins (CTDHs)...
September 21, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28935692/probing-structure-function-relationships-in-early-events-in-photosynthesis-using-a-chimeric-photocomplex
#7
Kenji V P Nagashima, Mai Sasaki, Kanako Hashimoto, Shinichi Takaichi, Sakiko Nagashima, Long-Jiang Yu, Yuto Abe, Kenta Gotou, Tomoaki Kawakami, Mizuki Takenouchi, Yuuta Shibuya, Akira Yamaguchi, Takashi Ohno, Jian-Ren Shen, Kazuhito Inoue, Michael T Madigan, Yukihiro Kimura, Zheng-Yu Wang-Otomo
The native core light-harvesting complex (LH1) from the thermophilic purple phototrophic bacterium Thermochromatium tepidum requires Ca(2+) for its thermal stability and characteristic absorption maximum at 915 nm. To explore the role of specific amino acid residues of the LH1 polypeptides in Ca-binding behavior, we constructed a genetic system for heterologously expressing the Tch. tepidum LH1 complex in an engineered Rhodobacter sphaeroides mutant strain. This system contained a chimeric pufBALM gene cluster (pufBA from Tch...
September 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28933362/male-infertility-the-effect-of-natural-antioxidants-and-phytocompounds-on-seminal-oxidative-stress
#8
REVIEW
Malik Adewoyin, Muhammad Ibrahim, Ramli Roszaman, Muhammad Lokman Md Isa, Nur Aizura Mat Alewi, Ainin Azwani Abdul Rafa, Mohd Nur Nasyriq Anuar
Defective sperm function has been identified as the most common cause of infertility. The objective of this study was to review recent findings on the effects of various antioxidants on male fertility. High amounts of poly unsaturated fatty acid are found in the mammalian spermatozoa membranes, thereby making them susceptible to lipid peroxidation. Although, free radicals and reactive oxygen species (ROS) play major roles in reproduction, they are strongly associated with oxidative stress. Furthermore, factors such as obesity, inflammation, pollutants and cigarette smoking are negatively correlated with spermatogenesis...
March 1, 2017: Diseases (Basel)
https://www.readbyqxmd.com/read/28932910/a-histone-deacetylase-gene-slhda3-acts-as-a-negative-regulator-of-fruit-ripening-and-carotenoid-accumulation
#9
Jun-E Guo, Zongli Hu, Xiaohui Yu, Anzhou Li, Fenfen Li, Yunshu Wang, Shibing Tian, Guoping Chen
SlHDA3 functions as an inhibitor and regulates tomato fruit ripening and carotenoid accumulation. Post-translational modifications, including histones acetylation, play a pivotal role in the changes of chromatin structure dynamic modulation and gene activity. The regulation of histone acetylation is achieved by the action of histone acetyltransferases and deacetylases, which play crucial roles in the regulation of transcription activation. There is an increasing research focus on histone deacetylation in crops, but the role of histone deacetylase genes (HDACs) in tomato has not been elucidated...
September 20, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28932778/data-on-the-density-of-xanthophores-in-a-whole-scale-of-goldfish-acclimated-to-white-or-black-background-color
#10
Kanta Mizusawa, Yutaka Yamamura, Satoshi Kasagi, José Miguel Cerdá-Reverter, Akiyoshi Takahashi
The data presented in this article are related to the research article entitled "Expression of genes for melanotropic peptides and their receptors for morphological color change in goldfish Carassius auratus" (Mizusawa et al., In press) [1]. This article describes data on the density of xanthophores in the scales of goldfish acclimated to white or black background color. To determine the effects of acclimation history during long-term background color adaptation, fish were transferred from a white tank to a white or black tank and vice versa halfway through the acclimation process...
October 2017: Data in Brief
https://www.readbyqxmd.com/read/28931584/use-of-a-super-child-approach-to-assess-the-vitamin-a-equivalence-of-moringa-oleifera-leaves-develop-a-compartmental-model-for-vitamin-a-kinetics-and-estimate-vitamin-a-total-body-stores-in-young-mexican-children
#11
Veronica Lopez-Teros, Jennifer Lynn Ford, Michael H Green, Guangwen Tang, Michael A Grusak, Luis Quihui-Cota, Tawanda Muzhingi, Mariela Paz-Cassini, Humberto Astiazaran-Garcia
Background: Worldwide, an estimated 250 million children <5 y old are vitamin A (VA) deficient. In Mexico, despite ongoing efforts to reduce VA deficiency, it remains an important public health problem; thus, food-based interventions that increase the availability and consumption of provitamin A-rich foods should be considered.Objective: The objectives were to assess the VA equivalence of (2)H-labeled Moringa oleifera (MO) leaves and to estimate both total body stores (TBS) of VA and plasma retinol kinetics in young Mexican children...
September 20, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28931206/metabolic-engineering-a-yeast-to-produce-astaxanthin
#12
Yu-Ju Lin, Jui-Jen Chang, Hao-Yeh Lin, Caroline Thia, Yi-Ying Kao, Chieh-Chen Huang, Wen-Hsiung Li
In this study, an astaxanthin-biosynthesis Kluyveromyces marxianus strain Sm23 was first constructed, which could produce 31µg/g DCW astaxanthin. Then, repeated genome integration of the key astaxanthin biosynthesis genes Hpchyb and bkt was done to increase gene copy number and astaxanthin yield. Four improved strains were obtained and the yield of astaxanthin and the total yield of carotenoids in a strain increased with the copy numbers of Hpchyb and bkt. To improve the yield further, the gene Hpchyb from Haematococcus pluvialis was modified by site-directed mutagenesis to increase the enzyme efficiency or/and to prevent the heterologous protein degradation by ubiquitination...
July 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28930152/proteomic-analysis-of-the-chlorophyta-dunaliella-new-strain-al-1-revealed-global-changes-of-metabolism-during-high-carotenoid-production
#13
Faten Ben Amor, Fatma Elleuch, Hajer Ben Hlima, Matthieu Garnier, Bruno Saint-Jean, Mohamed Barkallah, Chantal Pichon, Slim Abdelkafi, Imen Fendri
The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions...
September 20, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28929465/erratum-to-interaction-of-the-signaling-state-analog-and-the-apoprotein-form-of-the-orange-carotenoid-protein-with-the-fluorescence-recovery-protein
#14
Marcus Moldenhauer, Nikolai N Sluchanko, Neslihan N Tavraz, Cornelia Junghans, David Buhrke, Mario Willoweit, Leonardo Chiappisi, Franz-Josef Schmitt, Vladana Vukojević, Evgeny A Shirshin, Vladimir Y Ponomarev, Vladimir Z Paschenko, Michael Gradzielski, Eugene G Maksimov, Thomas Friedrich
In Fig. 1a in the original article, the amino acid side chains were incorrectly labeled in the structure representation of the orange carotenoid protein (OCP). The corrected figure is printed in this erratum.
September 19, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28929276/the-photosynthetic-responses-to-stocking-depth-and-algal-mat-density-in-the-farmed-seaweed-gracilaria-lemaneiformis-gracilariales-rhodophyta
#15
Heng Jiang, Dinghui Zou, Weizhou Chen, Yufeng Yang
The branches and mass of Gracilaria lemaneiformis increase with growth season, and the thalli sink to deeper depths with increasing biomass density during maricultivation. The changing depth and algal mat density may affect the physiology of the algae. In the present study, the photosynthetic behaviors regarding different biomass densities in G. lemaneiformis thalli collected from different stocking depths were determined, to examine how photosynthesis of this farmed alga was affected by the growth depths and algal mat densities...
September 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28929006/a-comparison-of-the-morphological-and-biochemical-characteristics-of-chlorella-sorokiniana-and-chlorella-zofingiensis-cultured-under-photoautotrophic-and-mixotrophic-conditions
#16
Siti Nor Ani Azaman, Norio Nagao, Fatimah M Yusoff, Sheau Wei Tan, Swee Keong Yeap
The responses of two species of microalgae, Chlorella sorokiniana and Chlorella zofingiensis, were compared regarding their morphological and biochemical properties under photoautotrophic and mixotrophic conditions. These microalgae were cultured under both conditions, and their crude ethanolic extracts were examined for their pigment and total phenolic contents. In addition, the microalgae's antioxidant activities were determined using a DPPH radical scavenging assay and a ferric reducing antioxidant power (FRAP) assay...
2017: PeerJ
https://www.readbyqxmd.com/read/28928905/fucoxanthin-a-marine-carotenoid-attenuates-%C3%AE-amyloid-oligomer-induced-neurotoxicity-possibly-via-regulating-the-pi3k-akt-and-the-erk-pathways-in-sh-sy5y-cells
#17
Jiajia Lin, Jie Yu, Jiaying Zhao, Ke Zhang, Jiachen Zheng, Jialing Wang, Chunhui Huang, Jingrong Zhang, Xiaojun Yan, William H Gerwick, Qinwen Wang, Wei Cui, Shan He
Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by neurofibrillary tangles, synaptic impairments, and loss of neurons. Oligomers of β-amyloid (Aβ) are widely accepted as the main neurotoxins to induce oxidative stress and neuronal loss in AD. In this study, we discovered that fucoxanthin, a marine carotenoid with antioxidative stress properties, concentration dependently prevented Aβ oligomer-induced increase of neuronal apoptosis and intracellular reactive oxygen species in SH-SY5Y cells...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28928521/comparative-analysis-of-carotenoid-content-in-momordica-cochinchinensis-cucurbitaceae-collected-from-australia-thailand-and-vietnam
#18
Dilani Wimalasiri, Robert Brkljača, Terrence J Piva, Sylvia Urban, Tien Huynh
Momordica cochinchinensis (Cucurbitaceae) is the richest source of lycopene and β-carotene of all known fruits but the influences of collection sites, variety and environment on carotenoid accumulation is unknown. This study analysed the carotenoid content of 44 M. cochinchinensis aril samples collected from Australia, Thailand and Vietnam using HPLC, UV-visible spectrophotometry and compared with the colorimetry method. The highest lycopene content was observed in samples collected from Ha Noi (7.76 mg/g) of Northern Vietnam and Lam Ha (6...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28927597/active-and-smart-biodegradable-packaging-based-on-starch-and-natural-extracts
#19
Carolina Medina-Jaramillo, Oswaldo Ochoa-Yepes, Celina Bernal, Lucía Famá
Active and smart biodegradable films from cassava starch and glycerol with 5wt.% of different natural extracts such as green tea and basil were obtained by casting. Their functional capacity as antioxidants and their physicochemical properties achieved from the incorporation of these types of extracts were evaluated. The content of phenolic compounds in the extracts led to films with significant antioxidant activity, being greater in the case of the system containing green tea extract. Color changes in both materials after immersion in different media (acid and basic) due to the presence of chlorophyll and carotenoids in the extracts were observed, but the film with basil extract reacted most notably to the different pH...
November 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28926945/association-between-dietary-carotenoid-intake-and-bone-mineral-density-in-korean-adults-aged-30-75-years-using-data-from-the-fourth-and-fifth-korean-national-health-and-nutrition-examination-surveys-2008-2011
#20
Gebereamanuel Meron Regu, Hyesook Kim, You Jin Kim, Ju Eun Paek, Gunjeong Lee, Namsoo Chang, Oran Kwon
Age-related bone loss is a major public health problem. This cross-sectional study examined the association between the dietary intake of carotenoids and bone mineral density (BMD). Data from 8022 subjects (3763 males and 4259 females) aged 30-75 years included in the Korean National Health and Nutrition Examination Survey (2008-2011) were analyzed. BMD was measured by dual-energy X-ray absorptiometry. Intake of carotenoids was estimated using 24-h dietary recall. In multiple linear analysis, after adjusting for covariates, lutein + zeaxanthin and β-cryptoxanthin intake was positively associated with total hip BMD in males and premenopausal women respectively, while β-carotene intake was positively correlated with femoral neck, total hip, and whole-body BMD in postmenopausal women...
September 16, 2017: Nutrients
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