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https://www.readbyqxmd.com/read/28724949/overexpression-of-slpre2-an-atypical-bhlh-transcription-factor-affects-plant-morphology-and-fruit-pigment-accumulation-in-tomato
#1
Zhiguo Zhu, Guoping Chen, Xuhu Guo, Wencheng Yin, Xiaohui Yu, Jingtao Hu, Zongli Hu
The basic helix-loop-helix (bHLH) proteins are a large family of transcription factors that control various developmental processes in eukaryotes, but the biological roles of most bHLH proteins are not very clear, especially in tomato. In this study, a PRE-like atypical bHLH gene was isolated and designated as SlPRE2 in tomato. SlPRE2 was highly expressed in immature-green fruits, moderately in young leaves, flowers, and mature-green fruits. To further research the function of SlPRE2, a 35 S:PRE2 binary vector was constructed and transformed into wild type tomato...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28722752/application-of-a-sensory-instrumental-tool-to-study-apple-texture-characteristics-shaped-by-altitude-and-time-of-harvest
#2
Mathilde Charles, Maria Laura Corollaro, Luigi Manfrini, Isabella Endrizzi, Eugenio Aprea, Angelo Zanella, Luca Corelli Grappadelli, Flavia Gasperi
BACKGROUND: Texture is important in the preferences of apple consumers. Of the pre-harvest factors affecting fruit quality and especially texture, altitude and subsequent climatic conditions are crucial, determining differences in the physiological mechanisms of fruit growth, ripening stage and chemical composition, as demonstrated by several studies. This work applies a detailed sensory-instrumental protocol developed in a previous paper(1) to investigate the impact of altitude, time of harvest and their cross-effect on sensory characteristics of apple, with a focus on texture...
July 19, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28720953/physico-chemical-changes-during-progressive-ripening-of-mango-mangifera-indica-l-cv-dashehari-under-different-temperature-regimes
#3
P P S Gill, S K Jawandha, Navdeep Kaur, Navprem Singh
The effect of different temperature regimes on ripening quality of mango cv. Dashehari was investigated. Fruits were kept at 20, 25 °C in temperature controlled chambers and at room temperature. Fruits were analyzed periodically for physico-chemical characteristics after every 24 h interval up to 168 h of ripening period. Development of soluble solid contents (SSC), flesh softening and physiological loss of weight of fruits occurred progressively during ripening. Progression of ripening changes in fruit were found to be less at 20 and 25 °C than at room temperature...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720093/solanum-nigrum-unripe-fruit-fraction-attenuates-adriamycin-resistance-by-down-regulating-multi-drug-resistance-protein-mdr-1-through-jak-stat-pathway
#4
Sankar Jagadeeshan, Diana David, S Jisha, S Manjula, S Asha Nair
BACKGROUND: Solanum nigrum, herbal plant that commonly grows in temperate climate zone, has been used as a traditional folk medicine whose ripen fruits were proven to exhibit anti-tumor properties. In traditional Chinese medicine, it has been used for centuries to cure inflammation, edema, mastitis and hepatic cancer and in the Ayurvedic system of traditional medicine in India, S. nigrum is applied against enteric diseases, ulcer, diarrhea and skin diseases. A methanolic glycosidic extract fraction of unripe fruit of S...
July 18, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28716425/characterization-and-quantification-of-flavonoids-and-organic-acids-over-fruit-development-in-american-cranberry-vaccinium-macrocarpon-cultivars-using-hplc-and-apci-ms-ms
#5
Yifei Wang, Jennifer Johnson-Cicalese, Ajay P Singh, Nicholi Vorsa
Cranberry flavonoids, including anthocyanins, flavonol glycosides and proanthocyanidins, and organic acids were characterized and quantified by HPLC and LC-MS/MS during fruit development and ripening in eight cranberry cultivars. Anthocyanin biosynthesis initiated at early fruit development and reached highest level in mature fruit, with significant differences between cultivars. Major flavonol glycosides, including the most abundant quercetin-3-galactoside and myricetin-3-galactoside, showed consistent concentrations during the season with moderate fluctuation, and were at similar levels in mature fruits of the eight cultivars...
September 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28713413/identification-of-conserved-and-novel-micrornas-in-blueberry
#6
Junyang Yue, Xiaohui Lu, Huan Zhang, Jiao Ge, Xueling Gao, Yongsheng Liu
MicroRNAs (miRNAs) are a class of small endogenous RNAs that play important regulatory roles in cells by negatively affecting gene expression at both transcriptional and post-transcriptional levels. There have been extensive studies aiming to identify miRNAs and to elucidate their functions in various plant species. In the present study, we employed the high-throughput sequencing technology to profile miRNAs in blueberry fruits. A total of 9,992,446 small RNA tags with sizes ranged from 18 to 30 nt were obtained, indicating that blueberry fruits have a large and diverse small RNA population...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28713399/metabolite-profiling-reveals-developmental-inequalities-in-pinot-noir-berry-tissues-late-in-ripening
#7
Amanda M Vondras, Mauro Commisso, Flavia Guzzo, Laurent G Deluc
Uneven ripening in Vitis vinifera is increasingly recognized as a phenomenon of interest, with substantial implications for fruit and wine composition and quality. This study sought to determine whether variation late in ripening (∼Modified Eichhorn-Lorenz stage 39) was associated with developmental differences that were observable as fruits within a cluster initiated ripening (véraison). Four developmentally distinct ripening classes of berries were tagged at cluster véraison, sampled at three times late in ripening, and subjected to untargeted HPLC-MS to measure variation in amino acids, sugars, organic acids, and phenolic metabolites in skin, pulp, and seed tissues separately...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28713396/system-level-and-granger-network-analysis-of-integrated-proteomic-and-metabolomic-dynamics-identifies-key-points-of-grape-berry-development-at-the-interface-of-primary-and-secondary-metabolism
#8
Lei Wang, Xiaoliang Sun, Jakob Weiszmann, Wolfram Weckwerth
Grapevine is a fruit crop with worldwide economic importance. The grape berry undergoes complex biochemical changes from fruit set until ripening. This ripening process and production processes define the wine quality. Thus, a thorough understanding of berry ripening is crucial for the prediction of wine quality. For a systemic analysis of grape berry development we applied mass spectrometry based platforms to analyse the metabolome and proteome of Early Campbell at 12 stages covering major developmental phases...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28703352/atpds-over-expression-in-tomato-exposing-unique-patterns-of-carotenoid-self-regulation-and-an-alternative-strategy-for-the-enhancement-of-fruit-carotenoid-content
#9
Ryan P McQuinn, Breanna Wong, James J Giovannoni
The regulation of plant carotenogenesis is an active research area for both biological discovery and practical implementation. In tomato (Solanum lycopersicum) we demonstrate additional bottlenecks exist in the poly-cis-transformation of phytoene to lycopene in the context of ripening-induced PSY1 expression and activity, and reveal phytoene desaturase (PDS), as a target for manipulation toward elevated lycopene content in maturing tomato fruit. Over-expression of Arabidopsis PDS, AtPDS, elevated PDS transcript abundance in all aerial tissues resulting in both altered carotenoid accumulation and associated pathway gene expression in a tissue-specific manner...
July 13, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28702036/a-feronia-like-receptor-kinase-regulates-strawberry-fragaria-%C3%A3-ananassa-fruit-ripening-and-quality-formation
#10
Meiru Jia, Ning Ding, Qing Zhang, Sinian Xing, Lingzhi Wei, Yaoyao Zhao, Ping Du, Wenwen Mao, Jizheng Li, Bingbing Li, Wensuo Jia
Ripening of fleshy fruits is controlled by a series of intricate signaling processes. Here, we report a FERONIA/FER-like receptor kinase, FaMRLK47, that regulates both strawberry (Fragaria × ananassa) fruit ripening and quality formation. Overexpression and RNAi-mediated downregulation of FaMRLK47 delayed and accelerated fruit ripening, respectively. We showed that FaMRLK47 physically interacts with FaABI1, a negative regulator of abscisic acid (ABA) signaling, and demonstrated that FaMRLK47 regulates fruit ripening by modulating ABA signaling, a major pathway governing strawberry fruit ripening...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28692131/vitis-vinifera-root-and-leaf-metabolic-composition-during-fruit-maturation-implications-of-defoliation
#11
Gerhard C Rossouw, Beverley A Orchard, Katja Šuklje, Jason P Smith, Celia Barril, Alain Deloire, Bruno P Holzapfel
Grapevine (Vitis vinifera) roots and leaves represent major carbohydrate and nitrogen (N) sources, either as recent assimilates, or mobilized from labile or storage pools. This study examined the response of root and leaf primary metabolism following defoliation treatments applied to fruiting vines during ripening. The objective was to link alterations in root and leaf metabolism to carbohydrate and N source functioning under conditions of increased fruit sink demand. Potted grapevine leaf area was adjusted near the start of véraison to 25 primary leaves per vine compared to 100 leaves for the control...
July 10, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28690624/transcriptome-wide-identification-of-novel-uv-b-and-light-modulated-flavonol-pathway-genes-controlled-by-vvimybf1
#12
Stefan Czemmel, Janine Höll, Rodrigo Loyola, Patricio Arce-Johnson, José Antonio Alcalde, José Tomás Matus, Jochen Bogs
Flavonols constitute a group of flavonoids with important photoprotective roles in plants. In addition, flavonol content and composition greatly influences fruit quality. We previously demonstrated that the grapevine R2R3-MYB transcription factor (TF) VviMYBF1 promotes flavonol accumulation by inducing the expression of flavonol synthase (VviFLS1/VviFLS4), a key step of the initial flavonol pathway. Despite this, gene networks underlying flavonol modification in grapevine including both structural and regulatory genes remain poorly understood...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28677256/xanthomonas-campestris-pv-musacearum-a-major-constraint-to-banana-plantain-and-enset-production-in-central-and-east-africa-over-the-past-decade
#13
Valentine Nakato, George Mahuku, Teresa Coutinho
Taxonomy: Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Xanthomonadales; Family Xanthomonadaceae; Genus Xanthomonas; currently classified as X. campestris pv. musacearum (Xcm). However, fatty acid methyl esters analysis, genetic and genomic evidence suggest that this pathogen is X. vasicola and resides in a separate pathovar. Isolation and detection: Xcm can be isolated on Yeast Peptone Glucose Agar (YPGA), Cellobiose Cephalexin Agar and Yeast Tryptone Sucrose Agar (YTSA) complemented with 5-flouro-uracil, cephalexin and cycloheximide to confer semi-selectivity...
July 5, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28674614/an-efp-browser-for-visualizing-strawberry-fruit-and-flower-transcriptomes
#14
Charles Hawkins, Julie Caruana, Jiaming Li, Chris Zawora, Omar Darwish, Jun Wu, Nadim Alkharouf, Zhongchi Liu
Wild strawberry Fragaria vesca is emerging as an important model system for the cultivated strawberry due to its diploid genome and availability of extensive transcriptome data and a range of molecular genetic tools. Being able to better utilize these tools, especially the transcriptome data, will greatly facilitate research progress in strawberry and other Rosaceae fruit crops. The electronic fluorescent pictograph (eFP) software is a useful and popular tool to display transcriptome data visually, and is widely used in other model organisms including Arabidopsis and mouse...
2017: Horticulture Research
https://www.readbyqxmd.com/read/28669186/volatile-compound-and-gene-expression-analyses-reveal-temporal-and-spatial-production-of-lox-derived-volatiles-in-pepino-solanum-muricatum-aiton-fruit-and-lox-specificity
#15
Carolina Contreras, Wilfried Schwab, Mechthild Mayershofer, Mauricio González-Agüero, Bruno G Defilippi
Lipoxygenase (LOX) is an important contributor to aroma compounds in most fresh produce; however, little is known about the LOX pathway in pepino (Solanum muricatum Aiton) fruit. We explored the LOX aroma compounds produced by the flesh and the peel and identified eight putative LOX genes expressed in both tissues during fruit growth and development during two consecutive seasons. This study shows that pepino produces C5, C6, and C9 LOX-derived compounds. Odorant C9 volatiles were produced during immature stages with a concomitant decrease when the fruit ripens, whereas C5 and C6 compounds were formed throughout ripening...
July 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28666960/analysis-of-virus-susceptibility-in-the-invasive-insect-pest-drosophila-suzukii
#16
Kwang-Zin Lee, Andreas Vilcinskas
The invasive insect pest Drosophila suzukii infests ripening fruits and causes massive agricultural damage in North America and Europe (Cini et al., 2012). Environmentally sustainable strategies are urgently needed to control the spread of this species, and entomopathogenic viruses offer one potential solution for global crop protection. Here we report the status of intrinsic and extrinsic factors that influence the susceptibility of D. suzukii to three model insect viruses: Drosophila C virus, Cricket paralysis virus and Flock house virus...
June 27, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28665213/twenty-years-of-european-union-support-to-gene-therapy-and-gene-transfer
#17
David Gancberg
For twenty years and throughout its research programmes, the European Union has supported the entire innovation chain for gene transfer and gene therapy. The fruits of this investment are ripening as gene therapy products are reaching the European market and as clinical trials are demonstrating the safety of this approach to treat previously untreatable diseases.
June 30, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28665023/physiological-molecular-and-ultrastructural-analyses-during-ripening-and-over-ripening-of-banana-musa-spp-aaa-group-cavendish-subgroup-fruit-suggest-characteristics-of-programmed-cell-death
#18
Maricruz Ramírez-Sánchez, Donald J Huber, C Eduardo Vallejos, Karen Kelley
BACKGROUND: Programmed cell death (PCD) is a part of plant development that has been studied for petal senescence and vegetative tissue but has not been thoroughly investigated for fleshy fruits. The purpose of this research was to examine ripening and over-ripening in banana fruit to determine if there were processes in common to previously described PCD. RESULTS: Loss of cellular integrity (over 40 %) and development of senescence related dark spot (SRDS) occurred after day 8 in banana peel...
June 30, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28662156/hydrogen-sulfide-alleviates-postharvest-ripening-and-senescence-of-banana-by-antagonizing-the-effect-of-ethylene
#19
Yun Ge, Kang-Di Hu, Sha-Sha Wang, Lan-Ying Hu, Xiao-Yan Chen, Yan-Hong Li, Ying Yang, Feng Yang, Hua Zhang
Accumulating evidence shows that hydrogen sulfide (H2S) acts as a multifunctional signaling molecule in plants, whereas the interaction between H2S and ethylene is still unclear. In the present study we investigated the role of H2S in ethylene-promoted banana ripening and senescence by the application of ethylene released from 1.0 g·L-1 ethephon solution or H2S with 1 mM sodium hydrosulfide (NaHS) as the donor or in combination. Fumigation with ethylene was found to accelerate banana ripening and H2S treatment effectively alleviated ethylene-induced banana peel yellowing and fruit softening in parallel with decreased activity of polygalacturonase (PG)...
2017: PloS One
https://www.readbyqxmd.com/read/28662086/abscisic-acid-pathway-involved-in-the-regulation-of-watermelon-fruit-ripening-and-quality-trait-evolution
#20
Yanping Wang, Shaogui Guo, Shouwei Tian, Jie Zhang, Yi Ren, Honghe Sun, Guoyi Gong, Haiying Zhang, Yong Xu
Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is a non-climacteric fruit. The modern sweet-dessert watermelon is the result of years of cultivation and selection for fruits with desirable qualities. To date, the mechanisms of watermelon fruit ripening, and the role of abscisic acid (ABA) in this process, has not been well understood. We quantified levels of free and conjugated ABA contents in the fruits of cultivated watermelon (97103; C. lanatus subsp. vulgaris), semi-wild germplasm (PI179878; C...
2017: PloS One
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