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https://www.readbyqxmd.com/read/28197155/dissecting-the-biochemical-and-transcriptomic-effects-of-a-locally-applied-heat-treatment-on-developing-cabernet-sauvignon-grape-berries
#1
Fatma Lecourieux, Christian Kappel, Philippe Pieri, Justine Charon, Jérémy Pillet, Ghislaine Hilbert, Christel Renaud, Eric Gomès, Serge Delrot, David Lecourieux
Reproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in grapevine berries response to high temperatures are poorly understood. Unlike recent data that addressed the effects on berry development of elevated temperatures applied at the whole plant level, the present work particularly focuses on the fruit responses triggered by direct exposure to heat treatment (HT). In the context of climate change, this work focusing on temperature effect at the microclimate level is of particular interest as it can help to better understand the consequences of leaf removal (a common viticultural practice) on berry development...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28196350/overexpression-of-the-carbohydrate-binding-module-from-solanum-lycopersicum-expansin-1-sl-exp1-modifies-tomato-fruit-firmness-and-botrytis-cinerea-susceptibility
#2
M A Perini, I N Sin, N M Villarreal, M Marina, A L T Powell, G A Martínez, P M Civello
Firmness, one of the major determinants of postharvest quality and shelf life of fruits is determined by the mechanical resistance imposed by the plant cell wall. Expansins (EXP) are involved in the non-hydrolytic metabolic disassembly of plant cell walls, particularly in processes where relaxation of the wall is necessary, such as fruit development and ripening. As many carbohydrate-associated proteins, expansins have a putative catalytic domain and a carbohydrate-binding module (CBM). Several strategies have been pursued to control the loss of fruit firmness during storage...
February 2, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28195221/transcriptome-analysis-around-the-onset-of-strawberry-fruit-ripening-uncovers-an-important-role-of-oxidative-phosphorylation-in-ripening
#3
Qing-Hua Wang, Cheng Zhao, Miao Zhang, Yu-Zhong Li, Yuan-Yue Shen, Jia-Xuan Guo
Although much progress has been made towards understanding the ripening of non-climacteric fruit using the strawberry as a model plant, the defined molecular mechanisms remain unclear. Here, RNA-sequencing was performed using four cDNA libraries around the onset of ripening, and a total of 31,793 unigenes and 335 pathways were annotated including the top five pathways, which were involved in ribosome, spliceosome, protein processing, plant-pathogen interaction and plant hormone signaling, and the important DEGs related to ripening were annotated to be mainly involved in protein translation and processing, sugar metabolism, energy metabolism, phytohormones, antioxidation, pigment and softening, especially finding a decreased trend of oxidative phosphorylation during red-coloring...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28177240/transcriptomic-analyses-of-ascorbic-acid-and-carotenoid-metabolites-influenced-by-root-restriction-during-grape-berry-development-and-ripening
#4
Feng Leng, Dan Dan Tang, Qiong Lin, Jin Ping Cao, Di Wu, Shiping Wang, Chongde Sun
Ascorbic acid (AsA) and carotenoids are recognized as crucial metabolites for various biological processes in plants. The contents of AsA and carotenoids in fruits are influenced by external environmental stimuli, such as water, temperature, light and hormones. However, it is still not clear whether it can be affected by root restriction (RR) treatment. In this study, 'Summer Black' grape berry (Vitis vinifera × V. labrusca) under RR and control treatments during development and ripening were used as materials...
February 8, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28153229/comparing-the-analytical-performances-of-micro-nir-and-ft-nir-spectrometers-in-the-evaluation-of-acerola-fruit-quality-using-pls-and-svm-regression-algorithms
#5
Cristina Malegori, Emanuel José Nascimento Marques, Sergio Tonetto de Freitas, Maria Fernanda Pimentel, Celio Pasquini, Ernestina Casiraghi
The main goal of this study was to investigate the analytical performances of a state-of-the-art device, one of the smallest dispersion NIR spectrometers on the market (MicroNIR 1700), making a critical comparison with a benchtop FT-NIR spectrometer in the evaluation of the prediction accuracy. In particular, the aim of this study was to estimate in a non-destructive manner, titratable acidity and ascorbic acid content in acerola fruit during ripening, in a view of direct applicability in field of this new miniaturised handheld device...
April 1, 2017: Talanta
https://www.readbyqxmd.com/read/28146285/cellulose-nanocrystal-reinforced-chitosan-coatings-for-improving-the-storability-of-postharvest-pears-under-both-ambient-and-cold-storages
#6
Zilong Deng, Jooyeoun Jung, John Simonsen, Yan Wang, Yanyun Zhao
Cellulose nanocrystal (CNC, 0%, 5%, and 10% w/w, in chitosan, dry basis) reinforced 2% chitosan aqueous coatings were evaluated for delaying the ripening and quality deterioration of postharvest green D'Anjou (Pyrus communis L.) and Bartlett (Pyrus communis L.) pears during 3 wk of ambient storage (20 ± 2 °C and 30 ± 2% RH) or 5 mo of cold storage (-1.1 °C and 90% RH), respectively. Ethylene and CO2 production, color, firmness, and internal fruit quality were monitored during both storage conditions. Moisture and gas barrier, antibacterial activity, and surface morphology of the derived films were also evaluated to investigate the mechanisms of delayed fruit ripening and quality deterioration...
February 1, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28146114/non-destructive-sensor-based-prediction-of-maturity-and-optimum-harvest-date-of-sweet-cherry-fruit
#7
Verena Overbeck, Michaela Schmitz, Michael Blanke
(1) Background: The aim of the study was to use innovative sensor technology for non-destructive determination and prediction of optimum harvest date (OHD), using sweet cherry as a model fruit, based on different ripening parameters. (2) Methods: Two cherry varieties in two growing systems viz. field and polytunnel in two years were employed. The fruit quality parameters such as fruit weight and size proved unsuitable to detect OHD alone due to their dependence on crop load, climatic conditions, cultural practices, and season...
January 31, 2017: Sensors
https://www.readbyqxmd.com/read/28139841/a-mechanistic-modelling-approach-to-understand-1-mcp-inhibition-of-ethylene-action-and-quality-changes-during-ripening-of-apples
#8
Sunny George Gwanpua, Bert E Verlinden, Maarten L A T M Hertog, Bart M Nicolai, Annemie H Geeraerd
BACKGROUND: 1-methylcyclopropene (1-MCP) inhibits ripening in climacteric fruit by blocking ethylene receptors, preventing ethylene from binding and eliciting its action. The objective of the current study was to use mathematical models to describe 1-MCP inhibition of apple fruit ripening, and to provide a tool for predicting ethylene production, and two important quality indicators of apple fruit, firmness and background colour. RESULTS: A model consisting of coupled differential equations describing 1-MCP inhibition of apple ripening was developed...
January 31, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28131060/proteomic-comparison-of-chelidonium-majus-l-latex-in-different-phases-of-plant-development
#9
Robert Nawrot, Rico Lippmann, Andrea Matros, Oskar Musidlak, Grzegorz Nowicki, Hans-Peter Mock
Chelidonium majus L. (Papaveraceae) latex is used in traditinonal folk medicine to treat papillae, warts, condylomas, which are visible effects of human papilloma virus (HPV) infections. The aim of this work was to provide new insights into the biology and medicinal use of C. majus milky sap in the flowering and fruit ripening period of the plant by comparing the protein content between samples collected on respective developmental stages using LC-MS-based label-free proteome approach. For quantification, the multiplexed LC-MS data were processed using comparative chemometric approach...
January 13, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28129739/itraq-based-protein-profiling-and-fruit-quality-changes-at-different-development-stages-of-oriental-melon
#10
Xiaoou Guo, Jingjing Xu, Xiaohui Cui, Hao Chen, Hongyan Qi
BACKGROUND: Oriental melon is one of the most popular crops for its nutritional and flavour quality. Components that determine melon quality, such as sugar, colour, texture, flavour and aroma, among other factors, accumulate in different developmental stages. Thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in the oriental melon is very important for advancing our understanding of oriental melon quality in the ripening processes. RESULTS: iTRAQ-based protein profiling was conducted on 'YuMeiren' oriental melon fruit at different developmental stages...
January 28, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28122266/identification-and-characterization-of-novel-micrornas-for-fruit-development-and-quality-in-hot-pepper-capsicum-annuum-l
#11
Zhoubin Liu, Yuping Zhang, Lijun Ou, Linyu Kang, Yuhua Liu, Junheng Lv, Ge Wei, Bozhi Yang, Sha Yang, Wenchao Chen, Xiongze Dai, Xuefeng Li, Shudong Zhou, Zhuqing Zhang, Yanqing Ma, Xuexiao Zou
MicroRNAs (miRNAs) are non-coding small RNAs which play an important regulatory role in various biological processes. Previous studies have reported that miRNAs are involved in fruit development in model plants. However, the miRNAs related to fruit development and quality in hot pepper (Capsicum annuum L.) remains unknown. In this study, small RNA populations from different fruit ripening stages and different varieties were compared using next-generation sequencing technology. Totally, 59 known miRNAs and 310 novel miRNAs were identified from four libraries using miRDeep2 software...
January 22, 2017: Gene
https://www.readbyqxmd.com/read/28119334/genome-wide-analysis-of-cis-regulatory-element-structure-and-discovery-of-motif-driven-gene-co-expression-networks-in-grapevine
#12
Darren Chern Jan Wong, Rodrigo Lopez Gutierrez, Gregory Alan Gambetta, Simone Diego Castellarin
Coordinated transcriptional and metabolic reprogramming ensures a plant's continued growth and survival under adverse environmental conditions. Transcription factors (TFs) act to modulate gene expression through complex cis-regulatory element (CRE) interactions. Genome-wide analysis of known plant CREs was performed for all currently predicted protein-coding gene promoters in grapevine (Vitis vinifera L.). Many CREs such as abscisic acid (ABA)-responsive, drought-responsive, auxin-responsive, and evening elements, exhibit bona fide CRE properties such as strong position bias towards the transcription start site (TSS) and over-representation when compared with random promoters...
January 24, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28112434/label-free-deep-shotgun-proteomics-reveals-protein-dynamics-during-tomato-fruit-tissues-development
#13
Jedrzej Szymanski, Yishai Levin, Alon Savidor, Dario Breitel, Louise Chappell-Maor, Uwe Heinig, Nadine Töpfer, Asaph Aharoni
Current innovations in mass-spectrometry-based technologies allow deep coverage of protein expression. Despite its immense value and in contrast to transcriptomics, only a handful of studies in crop plants engaged with global proteome assays. Here, we present large-scale shotgun proteomics profiling of tomato fruit across two key tissues and five developmental stages. A total of 7738 individual protein groups were identified and reliably measured at least in one of the analyzed tissues or stages. The depth of our assay enabled identification of 61 differentially expressed transcription factors including renowned ripening-related regulators and elements of ethylene signaling...
January 23, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28106715/seasonal-effects-on-the-population-morphology-and-reproductive-behavior-of-narnia-femorata-hemiptera-coreidae
#14
Lauren A Cirino, Christine W Miller
Many insects are influenced by the phenology of their host plants. In North Central Florida, Narnia femorata (Hemiptera: Coreidae) spends its entire life cycle living and feeding on Opuntia mesacantha ssp. lata. This cactus begins producing flower buds in April that lead to unripe green fruit in June that ripen into red fruit through December. Many morphological and behavioral characteristics of N. femorata are known to be affected by cactus phenology in a controlled laboratory setting, including the degree of sexual dimorphism and mating behavior...
January 17, 2017: Insects
https://www.readbyqxmd.com/read/28106252/investigation-into-the-role-of-endogenous-abscisic-acid-during-ripening-of-imported-avocado-cv-hass
#15
Marjolaine D Meyer, Gemma A Chope, Leon A Terry
BACKGROUND: The importance of ethylene in avocado ripening has been extensively studied. In contrast, little is known about the possible role of abscisic acid (ABA). The present work studied the effect of 1-MCP (0.3 μL L(-1) ), e + (®) Ethylene Remover and the combination thereof on the quality of imported avocado cv. Hass fruit stored for 7 days at 12 °C. Ethylene production, respiration, firmness, colour, heptose (C7) sugars and ABA concentrations in mesocarp tissue were measured throughout storage...
January 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28106249/targeted-forcing-improves-quality-nutritional-and-health-value-of-sweet-cherry-fruit
#16
Verena Overbeck, Michaela Schmitz, Michael Blanke
BACKGROUND: Trade and consumers alike require premium quality cherries with high nutritional and health values preferably of local origin. While early fruit imports cannot supply such fruit, a new technology of forcing cherry emerged for an early local supply by covering the crop in spring. In the apparent scarcity of data on the resulting fruit quality, fruit characteristics of forced cherries were compared with those without cover RESULTS: Size and weight of forced cherry fruit were successfully increased by 6-14%...
January 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28099767/a-comparative-study-of-phenolic-antioxidant-activity-and-flavonoid-biosynthesis-related-gene-expression-between-summer-and-winter-strawberry-cultivars
#17
Doori Park, Yeri Park, Young Hun Lee, Ik-Young Choi, Kyong Cheul Park, Sang Un Park, Byung Sup Kim, Young Rog Yeoung, Nam Il Park
Strawberry (Fragaria ananassa Duch.) possesses good antioxidant properties. Phenolic compounds in strawberries, such as anthocyanins and ellagic acid, mainly act as antioxidants. This study aimed to compare the phenolic content and expression patterns of genes involved in flavonoid biosynthesis between summer and winter strawberry cultivars affected by seasonal variation, degree of ripeness, and genotype. Antioxidant activity and the total content of phenols and flavonoids decreased with fruit ripening. Most notably, summer strawberry cultivars showed higher antioxidant activity than winter cultivars...
January 18, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28096780/molecular-breeding-of-a-novel-orange-brown-tomato-fruit-with-enhanced-beta-carotene-and-chlorophyll-accumulation
#18
Ranjith Kumar Manoharan, Hee-Jeong Jung, Indeok Hwang, Namhee Jeong, Kang Hee Kho, Mi-Young Chung, Ill-Sup Nou
BACKGROUND: Tomatoes provide a significant dietary source of the carotenoids, lycopene and β-carotene. During ripening, carotenoid accumulation determines the fruit colors while chlorophyll degradation. These traits have been, and continue to be, a significant focus for plant breeding efforts. Previous work has found strong evidence for a relationship between CYC-B gene expression and the orange color of fleshy fruit. Other work has identified a point mutation in SGR that impedes chlorophyll degradation and causes brown flesh color to be retained in some tomato varieties...
2017: Hereditas
https://www.readbyqxmd.com/read/28088087/ethylene-biosynthesis-and-perception-during-ripening-of-loquat-fruit-eriobotrya-japonica-lindl
#19
E Alos, A Martinez-Fuentes, C Reig, C Mesejo, M J Rodrigo, M Agustí, L Zacarías
In order to gain insights into the controversial ripening behavior of loquat fruits, in the present study we have analyzed the expression of three genes related to ethylene biosynthesis (ACS1, ACO1 and ACO2), two ethylene receptors (ERS1a and ERS1b), one signal transduction component (CTR1) and one transcription factor (EIL1) in peel and pulp of loquat fruit during natural ripening and also in fruits treated with ethylene (10μLL(-1)) and 1-MCP (10μLL(-1)), an ethylene action inhibitor. In fruits attached to or detached from the tree, a slight increase in ethylene production was detected at the yellow stage, but the respiration rate declined progressively during ripening...
December 19, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28081283/transcriptome-analysis-of-peach-prunus-persica-l-batsch-during-the-late-stage-of-fruit-ripening
#20
H F Pan, Y Sheng, Z H Gao, H L Chen, Y J Qi, X K Yi, G H Qin, J Y Zhang
Fruit ripening is a complex developmental process, the details of which remain largely unknown in fleshy fruits. In this paper, the fruit flesh of two peach varieties, "Zhongyou9" (a nectarine; Prunus persica L. Batsch) and its mutant "Hongyu", was analyzed by RNA-seq technology during two stages of ripening at 20-day intervals. One hundred and eighty significant upregulated and two hundred and thirty-five downregulated genes were identified in the experiment. Many of these genes were related to plant hormones, chlorophyll breakdown, accumulation of aroma and flavor volatiles, and stress...
December 23, 2016: Genetics and Molecular Research: GMR
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