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https://www.readbyqxmd.com/read/29683389/alterations-of-the-viroid-regions-that-interact-with-the-host-defense-genes-attenuate-viroid-infection-in-host-plant
#1
Charith Raj Adkar-Purushothama, Jean-Pierre Perreault
Understanding in intimate details how the viroid interaction with host's defense genes is a cornerstone for developing viroid resistant plants. In this present study, small RNAs (sRNA) derived from Potato spindle tuber viroid (PSTVd) were studied in silico in order to detect any interactions with the serine threonine kinase receptor, a transmembrane protein that plays a role in disease resistance in plants. Using molecular biology techniques, it was determined that PSTVd infection negatively affects at least three serine threonine kinase receptors as well as with three other genes that are known to be involved in the overall development of the tomato plants...
April 23, 2018: RNA Biology
https://www.readbyqxmd.com/read/29681560/high-tomato-and-tomato-product-consumption-is-protective-against-the-decline-in-handgrip-strength-among-japanese-adults-the-oroshisho-study
#2
Zhongyu Ren, Cong Huang, Haruki Momma, Yufei Cui, Kaijun Niu, Shota Sugiyama, Masakazu Nanno, Ryoichi Nagatomi
BACKGROUND: There is scarce epidemiological evidence regarding the relationships of the consumption of different types of vegetables or fruits with change in skeletal muscle strength. We prospectively examined the relationships among Japanese adults, using handgrip strength to assess skeletal muscle strength. METHODS: A 3-year study was carried out with 259 Japanese adults who were 22-68 years of age. The frequency of consumption of different types of vegetables or fruits were obtained using a validated self-administered dietary history questionnaire...
April 21, 2018: Journal of Epidemiology
https://www.readbyqxmd.com/read/29680904/abnormal-development-of-floral-meristem-triggers-defective-morphogenesis-of-generative-system-in-transgenic-tomatoes
#3
Inna Chaban, Marat Khaliluev, Ekaterina Baranova, Neonila Kononenko, Sergey Dolgov, Elena Smirnova
Parthenocarpy and fruit malformations are common among independent transgenic tomato lines, expressing genes encoding different pathogenesis-related (PR) protein and antimicrobal peptides. Abnormal phenotype developed independently of the expression and type of target genes, but distinctive features during flower and fruit development were detected in each transgenic line. We analyzed the morphology, anatomy, and cytoembryology of abnormal flowers and fruits from these transgenic tomato lines and compared them with flowers and fruits of wild tomatoes, line YaLF used for transformation, and transgenic plants with normal phenotype...
April 21, 2018: Protoplasma
https://www.readbyqxmd.com/read/29680049/nac-nor-mutations-in-tomato-penjar-accessions-attenuate-multiple-metabolic-processes-and-prolong-the-fruit-shelf-life
#4
Rakesh Kumar, Vajir Tamboli, Rameshwar Sharma, Yellamaraju Sreelakshmi
Several Penjar accessions of tomato grown in the Mediterranean exhibit prolonged shelf life and harbor alcobaca mutation. To uncover the metabolic basis underlying shelf life, we compared four Penjar accessions to Ailsa Craig. Three accessions bore alcobaca mutation, whereas the fourth was a novel NAC-NOR allele. Cuticle composition of Penjars varied widely during fruit ripening. All Penjars exhibited delayed ripening, prolonged on-vine and off-vine shelf life, low ethylene emission, and carotenoid levels. Metabolic profiling revealed shifts in Krebs cycle intermediates, amino acids, and γ-aminobutyric acid levels indicating the attenuation of respiration in Penjars during post-harvest storage...
September 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29679981/impact-of-three-different-plate-colours-on-short-term-satiety-and-energy-intake-a-randomized-controlled-trial
#5
Asli Akyol, Aylin Ayaz, Elif Inan-Eroglu, Cansu Cetin, Gulhan Samur
BACKGROUND: Plate colour was previously shown to alter the amount of food consumption due to its environmental effect on food intake. However, different studies have indicated that the effect of plate colour cannot be generalized. In light of this finding, the main objective of this study was to determine whether food consumption during an open buffet meal was different when using same-sized white, red or black plates. METHODS: This study was a crossover study conducted with 54 female participants aged 18-30 years with normal BMIs...
April 21, 2018: Nutrition Journal
https://www.readbyqxmd.com/read/29678917/genomic-inference-of-recombination-mediated-evolution-in-xanthomonas-euvesicatoria-and-x-perforans
#6
Mustafa O Jibrin, Neha Potnis, Sujan Timilsina, Gerald V Minsavage, Gary E Vallad, Pamela D Roberts, Jeffrey B Jones, Erica M Goss
Recombination is a major driver of evolution in bacterial populations because it can spread and combine independently evolved beneficial mutations. Recombinant lineages of plant bacterial pathogens are typically associated with colonization of novel hosts and emergence of new diseases. Here, we show that recombination between evolutionarily and phenotypically distinct plant pathogenic lineages generated recombinant lineages with unique combinations of pathogenicity and virulence factors. X. euvesicatoria ( Xe ) and X...
April 20, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29678861/the-tomato-mitogen-activated-protein-kinase-slmpk1-is-as-a-negative-regulator-of-the-high-temperature-stress-response
#7
Haidong Ding, Jie He, Yuan Wu, Xiao-Xia Wu, Cailin Ge, Yijun Wang, Silin Zhong, Edgar Peiter, Jian-Sheng Liang, Weifeng Xu
High temperature (HT) stress is a major environmental stress that limits plant growth and development. Mitogen-activated protein kinase (MAPK) cascades play key roles in plant growth and stress signaling, but their involvement in the HT stress response are poorly understood. Here, we describe a 47-kDa MBP-phosphorylated protein (p47-MBPK) activated in tomato (Solanum lycopersicum) leaves under HT, and identify it as SlMPK1 by MS/MS analysis. Silencing of SlMPK1 in transgenic tomato plants resulted in enhanced tolerance to HT, while overexpression resulted in reduced tolerance...
April 20, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29677447/prediction-models-for-assessing-lycopene-in-open-field-cultivated-tomatoes-by-means-of-a-portable-reflectance-sensor-cultivar-and-growing-season-effects
#8
Leonardo Ciaccheri, Lorenza Tuccio, Andrea A Mencaglia, Kalina Maja Sikorska-Zimny, Ewelina Hallmann, Artur Kowalski, Anna Grazia Mignani, Stanislaw Kaniszewski, Giovanni Agati
Reflectance spectroscopy represents a useful tool for the non-destructive assessment of tomato lycopene, even in the field. For this reason, a compact, low-cost, LED-based sensor has been developed to measure reflectance in the 400-750 nm spectral range. It was calibrated against wet chemistry and evaluated by Partial Least Squares (PLS) regression analyses. The lycopene prediction models were defined for two open-field cultivated red-tomato varieties: the processing oblong tomatoes of the cv. Calista (average weight: 76 g) and the fresh-consumption round tomatoes of the cv...
April 20, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29675821/integrated-effect-of-nutrients-from-a-recirculation-aquaponic-system-and-foliar-nutrition-on-the-yield-of-tomatoes-solanum-lycopersicum-l-and-solanum-pimpinellifolium
#9
Mariel Gullian Klanian, Mariana Delgadillo Diaz, Javier Aranda, Carolina Rosales Juárez
The objective of this study was to evaluate the potential of tomato plants to efficiently use the nitrogen (N) of a recirculation aquaponic system (RAS) and to evaluate the effects of foliar fertilization as a complement to the water nutrition on the growth of the two tomato cultivars. The significant effect of six macro- and seven micronutrients was evaluated on the plant growth and on the fruit yield. Two experiments were performed in a nutrient film aquaponic unit. The first experiment was designed to study the effects of foliar fertilization on the seedlings of two tomato cultivars Costoluto Genovese (CG) (Solanum lycopersicum L...
April 20, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29673247/ecological-relevance-of-the-major-allelochemicals-in-solanum-lycopersicum-roots-and-exudates
#10
Carlos Rial, Elisabeth Gómez, Rosa M Varela, Jose M G Molinillo, Francisco A Macias
The work described herein concerns the isolation of natural products from tomato roots. Previous studies have focused on the fruit and other parts of the plant have been less widely studied. Stigmasterol, bergapten and α-tomatine were isolated from tomato roots. Stigmasterol and α-tomatine were evaluated on wheat coleoptiles and α-tomatine was the most active compound. To confirm the phytotoxic activity, α-tomatine was tested on Lactuca sativa and two weeds (Lolium perenne and Echinochloa crus-galli), and it was active in all cases...
April 20, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29668443/development-and-validation-of-hplc-analytical-method-for-chlorantraniliprole-in-bulk-and-in-the-formulation
#11
Pankaj P Kapupara, Vandana Sorathiya, Ravi P Patel, Ketan V Shah
BACKGROUND: Chlorantraniliprole is widely used as a pesticide. It is only soluble in dimethyl formamide. However, most of the reported methods used acetonitrile and other solvents. AIM: To develop rapid, accurate, precise, and sensitive HPLC method for chlorantraniliprole. MATERIALS AND METHODS: 10 μG/mL of chlorantraniliprole containing solution was injected into the HPLC system and run in different solvent systems. 10 μG/mL solutions of chlorantraniprole was injected in a column with 20 μL microsyringe...
March 1, 2018: Folia Medica
https://www.readbyqxmd.com/read/29668111/efficient-in-planta-gene-targeting-in-tomato-using-geminiviral-replicons-and-the-crispr-cas9-system
#12
Tal Dahan-Meir, Shdema Filler-Hayut, Cathy Melamed-Bessudo, Samuel Bocobza, Henryk Czosnek, Asaph Aharoni, Avraham A Levy
Current breeding relies mostly on random mutagenesis and recombination to generate novel genetic variation. However, targeted genome editing is becoming an increasingly important tool for precise plant breeding. Using the CRISPR-Cas system combined with the bean yellow dwarf virus rolling circle replicon we optimized a method for targeted mutagenesis and gene replacement in tomato. The carotenoid isomerase (CRTISO) and phytoene synthase 1 (PSY1) genes from the carotenoid biosynthesis pathway were chosen as targets due to their easily detectable change of phenotype...
April 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29668051/evidence-for-a-specific-and-critical-role-of-mitogen-activated-protein-kinase-20-in-uni-to-binucleate-transition-of-microgametogenesis-in-tomato
#13
Lifei Chen, Dandan Yang, Youwei Zhang, Limin Wu, Yaoyao Zhang, Lei Ye, Changtian Pan, Yanjun He, Li Huang, Yong-Ling Ruan, Gang Lu
Mitogen-activated protein kinases (MAPKs) regulate diverse aspects of plant growth. However, their potential role in reproductive development remains elusive. Here, we discovered an unique role of SlMPK20, a plant-specific group D MAPK, in pollen development in tomato. RNAi-mediated suppression of SlMPK20 or its knockout using CRISPR/Cas9 significantly reduced or completely abolished pollen viability, respectively, with no effects on maternal fertility. Cell biology and gene expression analyses established that SlMPK20 exerts its role specifically at the uni-to-binucleate transition during microgametogenesis...
April 18, 2018: New Phytologist
https://www.readbyqxmd.com/read/29666396/tomato-uv-b-receptor-sluvr8-mediates-plant-acclimation-to-uv-b-radiation-and-enhances-fruit-chloroplast-development-via-regulating-slglk2
#14
Huirong Li, Yuxiang Li, Heng Deng, Xiaochun Sun, Anquan Wang, Xiaofeng Tang, Yongfeng Gao, Ning Zhang, Lihuan Wang, Shuzhang Yang, Yongsheng Liu, Songhu Wang
Plants utilize energy from sunlight to perform photosynthesis in chloroplast, an organelle that could be damaged by solar UV radiation. The ultraviolet-B (UV-B) photoreceptor UVR8 is required for UV-B perception and signal transduction. However, little is known about how UVR8 influence chloroplast development under UV-B radiation. Here, we characterized tomato UVR8 gene (SlUVR8) and our results indicated that SlUVR8 facilitate plant acclimation to UV-B stress by orchestrating expression of the UVB-responsive genes (HY5 and CHS) and accumulating UV-absorptive compounds...
April 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29666326/elucidation-of-diversity-among-f-1-hybrids-to-examine-heterosis-and-genetic-inheritance-for-horticultural-traits-and-tolcv-resistance-in-tomato
#15
Ramesh K Singh, N Rai, Amareesh K Singh, Prashant Kumar, T Chaubey, B Singh, Satya N Singh
Genetic diversity during prebreeding or postbreeding programme, is the key pillar to characterize the valuable traits and gene of interest. Whereas, superior or inferior heterotic performance of F1 depend on the diverse nature of their pedigree. Therefore, the aim of this study was to see the diversity between the interspecific crosses and effect of heterosis, and inheritance for the morphological traits and ToLCV resistance. All the 24 F1 interspecific crosses were classified into four clusters on the basis of morphological traitsas well as simple sequence repeat (SSR) markers...
March 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29665495/towards-better-microbial-safety-of-fresh-produce-chlorophyllin-based-photosensitization-for-microbial-control-of-foodborne-pathogens-on-cherry-tomatoes
#16
Egle Paskeviciute, Bernadeta Zudyte, Zivile Luksiene
The aim of this study is to evaluate the antimicrobial efficiency of Chlorophyllin-based photosensitization for microbial control of cherry tomatoes. Chlorophyllin-based photosensitization (1.5 × 10-4  M, 3 J/cm2 ) significantly (2.4 log) reduced the population of naturally distributed surface attached various mesophilic bacteria (microbiota) on tomatoes. Moreover, the population of thermoresistant strains of food pathogens Bacillus cereus and Listeria monocytogenes inoculated on tomatoes was reduced by 1...
April 11, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29665473/magnetic-metal-organic-frameworks-for-fast-and-efficient-solid-phase-extraction-of-six-sudan-dyes-in-tomato-sauce
#17
Xin-Ran Shi, Xue-Lei Chen, Yu-Lan Hao, Li Li, Hou-Jun Xu, Man-Man Wang
Magnetic solid-phase extraction is an effective and useful technique to preconcentrate trace analytes from food samples. In this study, a magnetic trimeric chromium octahedral metal-organic framework (Fe3 O4 -NH2 @MIL-101) was fabricated and characterized. Fe3 O4 -NH2 @MIL-101 was applied as an adsorbent of magnetic solid-phase extraction combined with high performance liquid chromatography to effectively isolate and simultaneously determine six Sudan dyes (Para Red, Sudan I-IV, and Sudan Red 7B) from tomato sauce...
April 10, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29665369/defective-rna-particles-derived-from-tomato-black-ring-virus-genome-interfere-with-the-replication-of-parental-virus
#18
Beata Hasiów-Jaroszewska, Julia Minicka, Aleksandra Zarzyńska-Nowak, Daria Budzyńska, Santiago F Elena
Tomato black ring virus (TBRV) is the only member of the Nepovirus genus that is known to form defective RNA particles (D RNAs) during replication. Here, de novo generation of D RNAs was observed during prolonged passages of TBRV isolates originated from Solanum lycopersicum and Lactuca sativa in Chenopodium quinoa plants. D RNAs of about 500 nt derived by a single deletion in the RNA1 molecule and contained a portion of the 5' untranslated region and viral replicase, and almost the entire 3' non-coding region...
April 14, 2018: Virus Research
https://www.readbyqxmd.com/read/29664905/erythritol-at-insecticidal-doses-has-harmful-effects-on-two-common-agricultural-crop-plants
#19
Sara E Scanga, Bilal Hasanspahič, Edin Zvorničanin, Jasmina Samardžić Koženjić, Andrew K Rahme, Jessica H Shinn-Thomas
Erythritol, a non-nutritive polyol, is the main component of the artificial sweetener Truvia®. Recent research has indicated that erythritol may have potential as an organic insecticide, given its harmful effects on several insects but apparent safety for mammals. However, for erythritol to have practical use as an insecticide in agricultural settings, it must have neutral to positive effects on crop plants and other non-target organisms. We examined the dose-dependent effects of erythritol (0, 5, 50, 500, 1000, and 2000 mM) on corn (Zea mays) and tomato (Solanum lycopersicum) seedling growth and seed germination...
2018: PloS One
https://www.readbyqxmd.com/read/29663868/imaging-mass-spectrometry-of-endogenous-polypeptides-and-secondary-metabolites-from-galls-induced-by-root-knot-nematodes-in-tomato-roots
#20
Eder Alves Barbosa, Mauro Ferreira Bonfim Junior, Carlos Bloch, Thales Lima Rocha, Gilbert Engler, Janice de Almeida Engler
Nematodes are devastating pests that infect most cultivated plant species and cause considerable agricultural losses worldwide. The understanding of metabolic adjustments induced during plant-nematode interaction is crucial to generate resistant plants or to select more efficient molecules to fight against this pest. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been used herein for in situ detection and mapping endogenous polypeptides and secondary metabolites from nematode-induced gall tissue...
April 17, 2018: Molecular Plant-microbe Interactions: MPMI
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