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https://www.readbyqxmd.com/read/28222174/slmapk3-enhances-tolerance-to-tomato-yellow-leaf-curl-virus-tylcv-by-regulating-salicylic-acid-and-jasmonic-acid-signaling-in-tomato-solanum-lycopersicum
#1
Yunzhou Li, Lei Qin, Jingjing Zhao, Tayeb Muhammad, Hehe Cao, Hailiang Li, Yan Zhang, Yan Liang
Several recent studies have reported on the role of mitogen-activated protein kinase (MAPK3) in plant immune responses. However, little is known about how MAPK3 functions in tomato (Solanum lycopersicum L.) infected with tomato yellow leaf curl virus (TYLCV). There is also uncertainty about the connection between plant MAPK3 and the salicylic acid (SA) and jasmonic acid (JA) defense-signaling pathways. The results of this study indicated that SlMAPK3 participates in the antiviral response against TYLCV. Tomato seedlings were inoculated with TYLCV to investigate the possible roles of SlMAPK1, SlMAPK2, and SlMAPK3 against this virus...
2017: PloS One
https://www.readbyqxmd.com/read/28221979/influence-of-transportation-conditions-and-postharvest-disinfection-treatments-on-microbiological-quality-of-fresh-market-tomatoes-cv-nemo-netta-in-a-south-african-supply-chain
#2
M S Sibomana, L W Ziena, S Schmidt, T S Workneh
Postharvest microbial spoilage due to suboptimal transportation and packaging conditions is a key concern for the South African tomato industry. This study investigated the influence of washing with tap water or aqueous disinfectant solutions (chlorinated and anolyte water) on the microbiological quality of tomatoes during storage after transportation in nonrefrigerated trucks along two supply routes when packaged in crates and boxes. Route 1 was 1,093 km from field to storage site, while route 2 was 1,057 km...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221974/evaluation-of-a-surface-sampling-method-for-recovery-of-human-noroviruses-prior-to-detection-using-reverse-transcription-quantitative-pcr
#3
Grace Tung-Thompson, Blanca I Escudero-Abarca, Janie Outlaw, Arnaud Ganee, Sylvanie Cassard, Claude Mabilat, Lee-Ann Jaykus
Human noroviruses are the most common cause of acute viral gastroenteritis, and the environmental persistence of these viruses contributes to their transmissibility. Environmental sampling is thus an important tool for investigating norovirus outbreaks and for assessing the effectiveness of cleaning and decontamination regimens. The purpose of this study was to evaluate a sampling material (wipes) for their efficacy at recovering human norovirus from hard surfaces and foods. Dilutions of a human norovirus GII...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221946/effect-of-power-levels-on-inactivation-of-escherichia-coli-o157-h7-salmonella-typhimurium-and-listeria-monocytogenes-in-tomato-paste-using-915-megahertz-microwave-and-ohmic-heating
#4
Sang-Soon Kim, Hye-Jung Sung, Hyo-Sun Kwak, In-Sun Joo, Jeong-Su Lee, Gwangpyo Ko, Dong-Hyun Kang
The effect of power levels on inactivation of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes in tomato paste was investigated using 915-MHz microwave heating (MW) and ohmic heating (OH). Heating uniformity, pathogen inactivation, and quality aspects were determined with 1.8-, 2.1-, 2.4-, and 3.0-kW MW and corresponding OH. GInaFit was used to analyze pathogen inactivation. The heating uniformity of MW-treated samples was inferior to that of OH-treated samples at low power levels of 1...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221891/microbial-quality-safety-and-pathogen-detection-by-using-quantitative-pcr-of-raw-salad-vegetables-sold-in-dhanbad-city-india
#5
Sujeet K Mritunjay, Vipin Kumar
Consumption of ready-to-eat fresh vegetables has increased worldwide, with a consequent increase in outbreaks caused by foodborne pathogens. In the Indian subcontinent, raw fresh vegetables are usually consumed without washing or other decontamination procedures, thereby leading to new food safety threats. In this study, the microbiological quality and pathogenic profile of raw salad vegetables was evaluated through standard protocols. In total, 480 samples (60 each of eight different salad vegetables) of cucumber, tomato, carrot, coriander, cabbage, beetroot, radish, and spinach were collected from different locations in Dhanbad, a city famous for its coal fields and often called the "Coal Capital of India...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221850/effectiveness-of-neutral-electrolyzed-water-on-incidence-of-fungal-rot-on-tomato-fruits-solanum-lycopersicum-l
#6
Alfonso Vásquez-López, Tania Villarreal-Barajas, Gerardo Rodríguez-Ortiz
We assessed the effect of neutral electrolyzed water (NEW) on the incidence of rot on tomato ( Solanum lycopersicum L.) fruits inoculated with Fusarium oxysporum , Galactomyces geotrichum , and Alternaria sp. at sites with lesions. The inoculated fruits were treated with NEW at 10, 30, and 60 mg liter(-1) active chlorine, with copper oxychloride fungicide, and with sterile distilled water (control) for 3, 5, and 10 min. In the experiment with F. oxysporum , 50 to 80% of the control fruits and 50 to 60% of the fruits treated with the fungicide exhibited symptoms of rot at the inoculated sites...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28220869/nitric-oxide-is-involved-in-the-oxytetracycline-induced-suppression-of-root-growth-through-inhibiting-hydrogen-peroxide-accumulation-in-the-root-meristem
#7
Qing-Xiang Yu, Golam Jalal Ahammed, Yan-Hong Zhou, Kai Shi, Jie Zhou, Yunlong Yu, Jing-Quan Yu, Xiao-Jian Xia
Use of antibiotic-contaminated manure in crop production poses a severe threat to soil and plant health. However, few studies have studied the mechanism by which plant development is affected by antibiotics. Here, we used microscopy, flow cytometry, gene expression analysis and fluorescent dyes to study the effects of oxytetracycline (OTC), a widely used antibiotic in agriculture, on root meristem activity and the accumulation of hydrogen peroxide (H2O2) and nitric oxide (NO) in the root tips of tomato seedlings...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220841/an-agrobacterium-tumefaciens-strain-with-gamma-aminobutyric-acid-transaminase-activity-shows-an-enhanced-genetic-transformation-ability-in-plants
#8
Satoko Nonaka, Tatsuhiko Someya, Sha Zhou, Mariko Takayama, Kouji Nakamura, Hiroshi Ezura
Agrobacterium tumefaciens has the unique ability to mediate inter-kingdom DNA transfer, and for this reason, it has been utilized for plant genetic engineering. To increase the transformation frequency in plant genetic engineering, we focused on gamma-aminobutyric acid (GABA), which is a negative factor in the Agrobacterium-plant interaction. Recent studies have shown contradictory results regarding the effects of GABA on vir gene expression, leading to the speculation that GABA inhibits T-DNA transfer. In this study, we examined the effect of GABA on T-DNA transfer using a tomato line with a low GABA content...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220591/transcriptome-analysis-of-root-knot-nematode-meloidogyne-incognita-infected-tomato-solanum-lycopersicum-roots-reveals-complex-gene-expression-profiles-and-metabolic-networks-of-both-host-and-nematode-during-susceptible-and-resistance-responses
#9
Neha Shukla, Rachita Yadav, Pritam Kaur, Simon Rasmussen, Shailendra Goel, Manu Agarwal, Arun Jagannath, Ramneek Gupta, Amar Kumar
Root knot nematodes (RKNs, Meloidogyne incognita) are economically important endoparasites having a wide-host range. We have taken a comprehensive transcriptomic approach to investigate the expression of both tomato and RKN genes in tomato roots at five infection time intervals from susceptible plants and two infection time intervals from resistant plants, grown under soil conditions. Differentially expressed genes during susceptible (1827-tomato, 462-RKN) and resistance (25-tomato, 160-RKN) interactions were identified...
February 21, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28220432/genome-wide-analysis-of-chromatin-accessibility-in-arabidopsis-infected-with-pseudomonas-syringae
#10
Yogendra Bordiya, Hong-Gu Kang
Changes in chromatin accessibility are an important aspect of the molecular changes that occur in eukaryotic cells responding to stress, and they appear to play a critical role in stress-induced transcriptional activation/reprogramming and epigenetic changes. In plants, pathogen infection has been shown to induce rapid and drastic transcriptional reprogramming; growing evidence suggests that chromatin remodeling plays an essential role in this phenomenon. The recent development of genomic tools to assess chromatin accessibility presents a significant opportunity to investigate the relationship between chromatin dynamicity and gene expression...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28220431/characterizing-the-immune-eliciting-activity-of-putative-microbe-associated-molecular-patterns-in-tomato
#11
Christopher R Clarke, Boris A Vinatzer
Detection of conserved microbe-associated molecular patterns (MAMPs), such as bacterial flagellin, is the first line of active defense in plants against pathogenic invaders. Successful pathogens must subvert this immune response to grow to high population density and cause disease. Flagellin from the bacterial pathogen Pseudomonas was the first identified bacterial MAMP and many species across the plant kingdom have sensitive perception systems for detecting the 22-amino acid epitope known as flg22. Tomato and several other solanaceous plants are also able to independently detect a second epitope of flagellin known as flgII-28...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28220423/leeix2-interactors-analysis-and-eix-mediated-responses-measurement
#12
Meirav Leibman-Markus, Silvia Schuster, Adi Avni
Plant-pathogen interactions involve a large number of wide regulatory systems, necessary for plant defense responses against pathogen attack. The fungal protein ethylene-inducing xylanase (EIX) elicits defense responses in specific cultivars of tobacco and tomato. The response to EIX is controlled by a single locus encoding for LeEIX2, a leucine-rich-repeat receptor-like-protein (LRR-RLP). As an RLP, LeEIX2 does not possess an obvious cytoplasmic signaling moiety such as a kinase domain. To study LeEIX2 mode of action, it is essential to identify the potential interactors involved after EIX perception...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28220324/virus-surveys-of-capsicum-spp-in-the-republic-of-benin-reveal-the-prevalence-of-pepper-vein-yellows-virus-and-the-identification-of-a-previously-uncharacterised-polerovirus-species
#13
Leonard Afouda, Daouda Kone, Valerien Zinsou, Laurence Dossou, Lawrence Kenyon, Stephan Winter, Dennis Knierim
Surveys were conducted in 2014 and 2015 in Southern and Northern Benin, respectively, to identify the viruses infecting peppers (Capsicum spp.). The samples were screened by ELISA for cucumber mosaic virus (CMV), pepper veinal mottle virus (PVMV), potato virus Y (PVY) and tomato yellow leaf curl virus (TYLCV). A generic reverse transcription PCR (RT-PCR) was used to test for the presence of poleroviruses. ELISA tests confirmed the prevalence of all viruses, while the RT-PCR detected pepper vein yellows virus (PeVYV) which is reported for the first time in Benin...
February 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28220137/proteomic-analysis-reveals-the-positive-effect-of-exogenous-spermidine-in-tomato-seedlings-response-to-high-temperature-stress
#14
Qinqin Sang, Xi Shan, Yahong An, Sheng Shu, Jin Sun, Shirong Guo
Polyamines are phytohormones that regulate plant growth and development as well as the response to environmental stresses. To evaluate their functions in high-temperature stress responses, the effects of exogenous spermidine (Spd) were determined in tomato leaves using two-dimensional electrophoresis and MALDI-TOF/TOF MS. A total of 67 differentially expressed proteins were identified in response to high-temperature stress and/or exogenous Spd, which were grouped into different categories according to biological processes...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28219523/combinatorial-effect-of-mutagenesis-and-medium-component-optimization-on-bacillus-amyloliquefaciens-antifungal-activity-and-efficacy-in-eradicating-botrytis-cinerea
#15
Fatma Masmoudi, Saoussen Ben Khedher, Amel Kamoun, Nabil Zouari, Slim Tounsi, Mohamed Trigui
This work is directed towards Bacillus amyloliquefaciens strain BLB371 metabolite production for biocontrol of fungal phytopathogens. In order to maximise antifungal metabolite production by this strain, two approaches were combined: random mutagenesis and medium component optimization. After three rounds of mutagenesis, a hyper active mutant, named M3-7, was obtained. It produces 7 fold more antifungal metabolites (1800AU/mL) than the wild strain in MC medium. A hybrid design was applied to optimise a new medium to enhance antifungal metabolite production by M3-7...
April 2017: Microbiological Research
https://www.readbyqxmd.com/read/28217134/proteasome-activity-profiling-uncovers-alteration-of-catalytic-%C3%AE-2-and-%C3%AE-5-subunits-of-the-stress-induced-proteasome-during-salinity-stress-in-tomato-roots
#16
Judit Kovács, Péter Poór, Farnusch Kaschani, Balakumaran Chandrasekar, Tram N Hong, Johana C Misas-Villamil, Bo T Xin, Markus Kaiser, Herman S Overkleeft, Irma Tari, Renier A L van der Hoorn
The stress proteasome in the animal kingdom facilitates faster conversion of oxidized proteins during stress conditions by incorporating different catalytic β subunits. Plants deal with similar kind of stresses and also carry multiple paralogous genes encoding for each of the three catalytic β subunits. Here, we investigated the existence of stress proteasomes upon abiotic stress (salt stress) in tomato roots. In contrast to Arabidopsis thaliana, tomato has a simplified proteasome gene set with single genes encoding each β subunit except for two genes encoding β2...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28214751/structure-and-distribution-of-the-sensilla-on-the-antennae-of-tuta-absoluta-lepidoptera-gelechiidae
#17
Thomas Bawin, France Collard, Lara De Backer, Boni Barthélémy Yarou, Philippe Compère, Frédéric Francis, François J Verheggen
The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a widespread devastating pest that develops on tomato and other economically important solanaceous crops. Current semiochemically-based management strategies still fail to significantly reduce damages and need to be improved. Here we describe under scanning and transmission electron microscopy the structure and distribution of the sensilla that are displayed on adult antennae. These were similar in size between males (3424.4±135.3μm) and females (3292...
February 1, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28214242/injury-and-recovery-of-escherichia-coli-atcc25922-cells-treated-by-high-hydrostatic-pressure-at-400-600%C3%A2-mpa
#18
Keitarou Kimura, Kazuya Morimatsu, Takashi Inaoka, Kazutaka Yamamoto
Escherichia coli cells were inactivated by high hydrostatic pressure (HHP) at 400-600 MPa and their recovery under various conditions was evaluated by colony counting and flow cytometer (FCM) analyses. The lag time in colony formation and an improved recovery of cells under less oxidative conditions (pyruvate addition to the medium and incubation in anaerobic conditions) were observed for HHP treated cells, which indicated that a significant portion of cells were injured and recovered during incubation after HHP treatment...
February 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28209810/genome-sequence-of-southern-tomato-virus-in-asymptomatic-tomato-sweet-hearts
#19
Ricardo I Alcalá-Briseño, Sevgi Coşkan, Maria A Londoño, Jane E Polston
The genome sequence of Southern tomato virus in asymptomatic Solanum lycopersicum 'Sweet Hearts' (STV-Florida) in Florida was assembled from small RNAs sequenced by Illumina RNA-seq. The STV-Florida genome shared 99.0 to 99.9% similarity with full genome sequences from Bangladesh, China, Mexico, and the United States (Mississippi and North Carolina).
February 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28209809/macrophage-to-myofibroblast-transition-contributes-to-interstitial-fibrosis-in-chronic-renal-allograft-injury
#20
Ying-Ying Wang, Hong Jiang, Jun Pan, Xiao-Ru Huang, Yu-Cheng Wang, Hong-Feng Huang, Ka-Fai To, David J Nikolic-Paterson, Hui-Yao Lan, Jiang-Hua Chen
Interstitial fibrosis is an important contributor to graft loss in chronic renal allograft injury. Inflammatory macrophages are associated with fibrosis in renal allografts, but how these cells contribute to this damaging response is not clearly understood. Here, we investigated the role of macrophage-to-myofibroblast transition in interstitial fibrosis in human and experimental chronic renal allograft injury. In biopsy specimens from patients with active chronic allograft rejection, we identified cells undergoing macrophage-to-myofibroblast transition by the coexpression of macrophage (CD68) and myofibroblast (α-smooth muscle actin [α-SMA]) markers...
February 16, 2017: Journal of the American Society of Nephrology: JASN
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