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https://www.readbyqxmd.com/read/28346401/targeted-base-editing-in-rice-and-tomato-using-a-crispr-cas9-cytidine-deaminase-fusion
#1
Zenpei Shimatani, Sachiko Kashojiya, Mariko Takayama, Rie Terada, Takayuki Arazoe, Hisaki Ishii, Hiroshi Teramura, Tsuyoshi Yamamoto, Hiroki Komatsu, Kenji Miura, Hiroshi Ezura, Keiji Nishida, Tohru Ariizumi, Akihiko Kondo
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
March 27, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28345329/lycopene-extracts-from-different-tomato-based-food-products-induce-apoptosis-in-cultured-human-primary-prostate-cancer-cells-and-regulate-tp53-bax-and-bcl-2-transcript-expression
#2
Nathalia da Costa Pereira Soares, Clara Lima Machado, Bruno Boquimpani Trindade, Ingridy Celestino do Canto Lima, Etel Rodrigues Pereira Gimba, Anderson Junger Teodoro, Christina Takiya, Radovan Borojevic
Carotenoids are the main tomato components, especially lycopene. Lycopene is more bioavailable in tomato processed products than in raw tomatos, since formation of lycopene cis-isomers during food processing and storage may increase its biological activity. In the current study, we evaluated the influence of lycopene extracts (5 mg / mL) from different tomato-based food products (paste, sauce, extract and ketchup) on cell viability and apoptosis on primary human prostate cancer cells (PCa cels) for 96h. Using MTT assay, we observed a significant decrease on primary PCa cell viability upon treatment with lycopene extracted from either 4 tomato-based food products...
February 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28345196/flupyradifurone-effectively-manages-whitefly-bemisia-tabaci-med-hemiptera-aleyrodidae-and-tomato-yellow-leaf-curl-virus-tylcv-in-tomato
#3
Emmanouil Roditakis, Marianna Stavrakaki, Maria Grispou, Aikaterini Achimastou, Χavier Van Waetermeulen, Ralf Nauen, Anastasia Tsagkarakou
BACKGROUND: The cotton whitefly Bemisia tabaci (Gennadius) is among the most important pests of numerous crops and a vector of >100 plant viruses, causing significant crop losses worldwide. Managing the pest as well as inhibiting the transmission of major viruses such as Tomato yellow leaf curl virus (TYLCV) is of utmost importance for sustainable yields. The efficacy on both whiteflies and virus transmission of the novel systemic butenolide insecticide flupyradifurone, was investigated in this study...
March 27, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28343941/characterization-of-culturable-bacteria-isolated-from-hot-springs-for-plant-growth-promoting-traits-and-effect-on-tomato-lycopersicon-esculentum-seedling
#4
Kinjal Samir Patel, Jinal Hardik Naik, Sejal Chaudhari, Natarajan Amaresan
To elucidate the functional diversity of hot spring bacteria, 123 bacteria were isolated and screened for evaluating their multifunctional plant growth promoting (PGP) properties. The antagonistic activity against different phytopathogens showed the presence of a high amount of biocontrol bacteria in the hot springs. During screening for PGP properties, 61.0% isolates showed production of indole acetic acid and 23.6% showed inorganic phosphate solubilization qualitatively. For production of extracellular enzymes, it was found that 61...
March 23, 2017: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/28343383/a-new-nematotoxic-indoloditerpenoid-produced-by-gymnoascus-1-reessii-za-130
#5
Ting Liu, Susan L F Meyer, David J Chitwood, Kamlesh R Chauhan, Dan Dong, TaoTao Zhang, Jun Li, Wei-Cheng Liu
Chemical investigation of the fungal strain Gymnoascus reessii za-130, which was previously isolated from the rhizosphere of tomato plants infected by the root-knot nematode Meloidogyne incognita, led to the isolation and identification of a new indoloditerpenoid metabolite designated as gymnoascole acetate. Its structure was established by spectroscopic methods including 1D- and 2D-NMR and MS analysis. Gymnoascole acetate demonstrated strong adverse effects on M. incognita second-stage juvenile (J2) viability; exposure to 36 μg/mL for 24 h induced 100% paralysis of J2 (EC50 = 47...
March 25, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28343353/development-reproduction-survival-and-demographic-patterns-of-tuta-absoluta-meyrick-lepidoptera-gelechiidae-on-different-commercial-tomato-cultivars
#6
F S Krechemer, L A Foerster
The increase in the production of tomato, Solanum lycopersicon Mill. (Solanaceae), has favored the proliferation of pests, especially Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). In this study, the development, reproduction, survival, and demographic parameters of T. absoluta reared on six commercial tomato cultivars (Cherry, Cordilheira, Giuliana, Nemoneta, Paron, and Santa Clara) were evaluated. Tuta absoluta completed its development in all tomato cultivars. Development from newly hatched caterpillar to newly emerged adult varied between 24...
March 25, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28342612/can-by-products-replace-conventional-ingredients-in-concentrate-of-dairy-goat-diet
#7
M Romero-Huelva, M A Ramírez-Fenosa, R Planelles-González, P García-Casado, E Molina-Alcaide
Intensive dairy goat production in the Mediterranean basin is based on imported conventional ingredients to be included in concentrates. Fourteen Murciano-Granadina goats in the middle of the third lactation were allocated into 2 groups of 7 animals each fed, respectively, a control diet based on alfalfa hay and concentrate in a 40:60 ratio, and a diet in which the concentrate included tomato fruits, citrus pulp, brewer's grain and brewer's yeast (T100CBY) to study the effect of diet on nutrient utilization, ruminal fermentation, purine derivatives excretion in urine, milk yield and composition, and methane emissions...
March 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28342049/safety-assessment-of-chromafenozide-residue-level-with-decline-study-on-tomato-in-egypt
#8
Ehab Abdelraheem, Mohamed Arief, Somaia G Mohammad, Wayne Jiang
The objective of this study is to perform a safety assessment of chromafenozide residue level on tomato at the Egyptian national level. An open field decline study of chromafenozide on tomato was performed. The theoretical maximum daily intake (TMDI) of chromafenozide was calculated for assessing the chronic dietary exposure indicating that the ADI value of chromafenozide (0.27 mg/kg bw/day) was not exceeded. As a result, the safety assessment of chromafenozide residue levels was attained. A validated method of the QuEChERS approach followed by HPLC-DAD analysis was used to determine the chromafenozide residues...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28342034/molecular-characterization-of-a-new-begomovirus-infecting-mirabilis-jalapa-in-northern-india
#9
Aditya Kulshreshtha, Poonam Roshan, Dolly Sharma, Vipin Hallan
Begomoviruses are whitefly-transmitted single-stranded DNA viruses that are responsible for considerable economic losses. A begomovirus, alphasatellite and betasatellite were characterized in a Mirabilis jalapa plant exhibiting severe leaf curling and mottling symptoms. The complete viral genome shared highest sequence identity of 87% with pedilanthus leaf curl virus (AM712436), reported from Pakistan. Additionally, the viral genome was 84% identical to that of chilli leaf curl India virus (KX951415) and 83% identical to that of tobacco curly shoot virus (GU1999584), which were previously reported to infect M...
March 24, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28340727/a-quantitative-approach-for-cd-cu-fe-and-mn-through-laser-ablation-imaging-for-evaluating-the-translocation-and-accumulation-of-metals-in-sunflower-seeds
#10
Gustavo de S Pessôa, Cícero A Lopes Júnior, Katherine C Madrid, Marco A Z Arruda
The uptake and accumulation of Cd in sunflower seeds represents an important pathway for imputing potentially toxic metals into human and animal food. In this way, bioimaging of Cd and micronutrients (Cu, Fe and Mn) in the seeds of sunflower grown in soil contaminated with Cd are performed. For this task, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is used in quantitative approach, considering four groups: precursor, control, Cd-low (50mg) and Cd-high (700mg). For attaining our proposals, ICP-MS (nebulizer and auxiliary flow rates and radiofrequency power) and LA (laser intensity, frequency and spot size) parameters were optimized, and the analytical signal improved to 197%, 217%, 232%, and 283%, for (57)Fe, (112)Cd, (55)Mn and (63)Cu, respectively...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28340275/mini-tomato-genotypes-resistant-to-the-silverleaf-whitefly-and-to-two-spotted-spider-mites
#11
G M Maciel, R S Almeida, J P R da Rocha, V Andaló, G R Marquez, N C Santos, R R Finzi
The mini tomato production has expanded, becoming an amazing alternative for enterprise. Despite all commercial potential, the cultivation has the occurrence of pests as main obstacle during the crop development. Nowadays, there are no researches that aimed obtaining genotypes with high acylsugar content, capable of providing a broad-spectrum resistance to pests. This study aimed the selection of mini tomato genotypes, with high acylsugar content, and checking the resistance level to the silverleaf whitefly [Bemisia tabaci (Gennadius)] and to the two-spotted spider mites (Tetranychus urticae Koch)...
March 22, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28339724/three-cis-regulatory-motifs-auxre-mycrs1-and-mycrs2-are-required-for-modulating-the-auxin-and-mycorrhiza-responsive-expression-of-a-tomato-gh3-gene
#12
Xiao Chen, Dehua Liao, Xiaofeng Yang, Minjie Ji, Shuangshuang Wang, Mian Gu, Aiqun Chen, Guohua Xu
Auxin is well known to be a key regulator that acts in almost all physiological processes during plant growth, and in interactions between plants and microbes. However, to date, the regulatory mechanisms underlying auxin-mediated plant-arbuscular mycorrhizal (AM) fungi symbiosis have not been well deciphered. Previously we identified a GH3 gene, SlGH3.4, strongly responsive to both auxin induction and mycorrhizal symbiosis. Here, we reported a refined dissection of the SlGH3.4 promoter activity using the β-glucuronidase (GUS) reporter...
February 21, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28338918/integrated-mirna-and-mrna-expression-profiling-reveals-the-response-regulators-of-a-susceptible-tomato-cultivar-to-early-blight-disease
#13
Deepti Sarkar, Ranjan Kumar Maji, Sayani Dey, Arijita Sarkar, Zhumur Ghosh, Pallob Kundu
Early blight, caused by the fungus Alternaria solani, is a devastating foliar disease of tomatoes, causes massive yield loss each year worldwide. Molecular basis of the compatible host-pathogen interaction was elusive. We adopted next generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed during Alternaria-stress in tomato. Some of the interesting findings were also validated by alternative techniques. Our analysis revealed 181 known-miRNAs, belonging to 121 miRNA families, of which 67 miRNAs showed at least 2-fold change in expression level with the majority being downregulated...
March 3, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28336916/evaluation-of-the-effects-of-irrigation-and-fertilization-on-tomato-fruit-yield-and-quality-a-principal-component-analysis
#14
Xiukang Wang, Yingying Xing
Irrigation and fertilization are key practices for improving the fruit quality and yield of vegetables grown in greenhouses. We carried out an experiment in a solar greenhouse spanning three consecutive growing seasons to evaluate the effects of irrigation and fertilization on the fruit yield and quality, water use efficiency (WUE) and fertilizer partial factor productivity (PFP) of tomatoes. Interactions between irrigation and fertilization treatments and individual factors of irrigation and fertilization significantly (p < 0...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28335772/production-of-s-2-aminobutyric-acid-and-s-2-aminobutanol-in-saccharomyces-cerevisiae
#15
Nora Weber, Anaëlle Hatsch, Ludivine Labagnere, Harald Heider
BACKGROUND: Saccharomyces cerevisiae (baker's yeast) has great potential as a whole-cell biocatalyst for multistep synthesis of various organic molecules. To date, however, few examples exist in the literature of the successful biosynthetic production of chemical compounds, in yeast, that do not exist in nature. Considering that more than 30% of all drugs on the market are purely chemical compounds, often produced by harsh synthetic chemistry or with very low yields, novel and environmentally sound production routes are highly desirable...
March 23, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28335727/overexpression-of-snapdragon-delila-del-gene-in-tobacco-enhances-anthocyanin-accumulation-and-abiotic-stress-tolerance
#16
Aung Htay Naing, Kyeung Il Park, Trinh Ngoc Ai, Mi Young Chung, Jeung Sul Han, Young-Wha Kang, Ki Byung Lim, Chang Kil Kim
BACKGROUND: Rosea1 (Ros1) and Delila (Del) co-expression controls anthocyanin accumulation in snapdragon flowers, while their overexpression in tomato strongly induces anthocyanin accumulation. However, little data exist on how Del expression alone influences anthocyanin accumulation. RESULTS: In tobacco (Nicotiana tabacum 'Xanthi'), Del expression enhanced leaf and flower anthocyanin production through regulating NtCHS, NtCHI, NtF3H, NtDFR, and NtANS transcript levels...
March 23, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28335196/gold-nanomaterial-uptake-from-soil-is-not-increased-by-arbuscular-mycorrhizal-colonization-of-solanum-lycopersicum-tomato
#17
Jonathan D Judy, Jason K Kirby, Mike J McLaughlin, Timothy Cavagnaro, Paul M Bertsch
Bioaccumulation of engineered nanomaterials (ENMs) by plants has been demonstrated in numerous studies over the past 5-10 years. However, the overwhelming majority of these studies were conducted using hydroponic systems and the degree to which the addition of the biological and chemical components present in the soil might fundamentally alter the potential of plant bioaccumulation of ENMs is unclear. Here, we used two genotypes of Solanum lycopersicum (tomato), reduced mycorrhizal colonization (rmc), a mutant which does not allow arbuscular mycorrhizal fungi (AMF) colonization, and its progenitor, 76R, to examine how colonization by AMF alters trends of gold ENM bioaccumulation from a natural soil...
April 13, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334339/insecticidal-effects-on-the-spatial-progression-of-tomato-yellow-leaf-curl-virus-and-movement-of-its-whitefly-vector-in-tomato
#18
M Dempsey, D G Riley, R Srinivasan
Commercial management of whitefly-transmitted Tomato yellow leaf curl virus (TYLCV) typically relies on insecticide control of whitefly vectors as a first line of defense. We quantified this effect in crop tunnel studies, with validation in a tomato field setting. Tomato yellow leaf curl virus-infected and Bemisia tabaci (Gennadius)-infested source plants were planted at the beginning of tunneled rows to serve as inoculum source, so that movement of whiteflies and TYLCV symptoms could be tracked down the length of the tunnel over time...
March 8, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28334330/survivorship-of-z-pheromone-race-european-corn-borer-lepidoptera-crambidae-on-a-range-of-host-plants-varying-in-defensive-chemistry
#19
Kelsey E Fisher, Charles E Mason, J Lindsey Flexner, Judith Hough-Goldstein, John H McDonald
The European corn borer, Ostrinia nubilalis (Hübner), was introduced in North America in the early 1900s and became a major pest of corn. After its introduction, it was found on > 200 other plant hosts, but corn remained its primary host. Early life history studies indicated that European corn borer had the potential of a wide host range. For nearly 80 yr before the introduction of Bt corn, the European corn borer was a major pest of corn in North America. This study investigated the growth and survivorship of the Z-pheromone race European corn borer on a range of hosts that vary in defensive chemistries and historic degree of infestation to better understand the current host plant range of Z-pheromone race of O...
March 3, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28334249/lethal-and-sublethal-effects-of-some-chemical-and-biological-insecticides-on-tuta-absoluta-lepidoptera-gelechiidae-eggs-and-neonates
#20
Z Nozad-Bonab, M J Hejazi, Sh Iranipour, M Arzanlou
Tuta absoluta (Meyrick) is one of the serious pests of tomatoes. This study was conducted to evaluate the effects of some chemical and biological insecticides on T. absoluta . The insecticides tested were diazinon, dichlorvos, chlorantraniliprole, deltamethrin, acetamiprid, imidacloprid, spinosad, abamectin, indoxacarb, Bacillus thuringiensis Berliner, Beauveria bassiana (Balsamo) Vuillemin, and Metarhizium anisopliae (Metschnikoff) Sorokin. Sublethal effects for the most effective insecticides tested, namely, abamectin, spinosad, chlorantraniliprole, and indoxacarb were assessed, and life table parameters were calculated...
March 13, 2017: Journal of Economic Entomology
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