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https://www.readbyqxmd.com/read/28829889/stability-of-annual-biomass-energy-production-of-elephant-grass-pennisetum-purpureum-schum-genotypes-in-the-northern-region-of-the-rio-de-janeiro-state-brazil
#1
M S B Araújo, R F Daher, B R S Menezes, G A Gravina, V B Silva, A T Amaral Júnior, E V Rodrigues, B O Almeida, N J Ponciano
The objectives of this study were to estimate the stability of dry biomass production of elephant grass genotypes under an annual harvest regime, in soil-climatic conditions of the Northern region of the Rio de Janeiro State, Brazil, and to compare methodologies for stability analyses of Yates and Cochran (YC), Plaisted and Peterson (PP), Annicchiarico (ANN), Lin and Binns (LB), Huenh (HU), and Kang and Phan (KP). A randomized block design with 83 treatments and two replicates was adopted. Four annual harvests were performed (2012-2015) and dry matter yield (DMY, t...
August 17, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28827822/comparative-gene-expression-study-and-pathway-analysis-of-the-human-iris-and-the-retinal-pigment-epithelium
#2
Anna Bennis, Jacoline B Ten Brink, Perry D Moerland, Vivi M Heine, Arthur A Bergen
BACKGROUND: The retinal pigment epithelium (RPE) is a neural monolayer lining the back of the eye. Degeneration of the RPE leads to severe vision loss in, so far incurable, diseases such as age-related macular degeneration and some forms of retinitis pigmentosa. A promising future replacement therapy may be autologous iris epithelial cell transdifferentiation into RPE in vitro and, subsequently, transplantation. In this study we compared the gene expression profiles of the iris epithelium (IE) and the RPE...
2017: PloS One
https://www.readbyqxmd.com/read/28827667/lapsyn-controls-branch-extension-and-positioning-of-astrocyte-like-glia-in-the-drosophila-optic-lobe
#3
Benjamin Richier, Cristina de Miguel Vijandi, Stefanie Mackensen, Iris Salecker
Astrocytes have diverse, remarkably complex shapes in different brain regions. Their branches closely associate with neurons. Despite the importance of this heterogeneous glial cell type for brain development and function, the molecular cues controlling astrocyte branch morphogenesis and positioning during neural circuit assembly remain largely unknown. We found that in the Drosophila visual system, astrocyte-like medulla neuropil glia (mng) variants acquire stereotypic morphologies with columnar and layered branching patterns in a stepwise fashion from mid-metamorphosis onwards...
August 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28827628/phytotoxic-dioxolanones-are-potential-virulence-factors-in-the-infection-process-of-guignardia-bidwellii
#4
Iris Buckel, Lars Andernach, Anja Schüffler, Meike Piepenbring, Till Opatz, Eckhard Thines
Phytotoxic dioxolanones from Guignardia bidwellii can be described as potential virulence factors which cause the formation of lesions upon an infection by G. bidwellii. The toxin guignardic acid was found in planta of G. bidwellii-infected Vitis vinifera leaves, whereas no phytotoxic dioxolanones were detected in uninfected leaf material. Secondary metabolism analyses of further phytopathogenic fungi from the genus Guignardia led to the observation that all species investigated can produce the phytotoxins known from G...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28827387/retraction-hypoxia-inducible-factor-1%C3%AE-dependent-protection-from-intestinal-ischemia-reperfusion-injury-involves-ecto-5-nucleotidase-cd73-and-the-a2b-adenosine-receptor
#5
Melanie L Hart, Iris C Gorzolla, Jens Schittenhelm, Julee H Dalton, Holger K Eltzschig
No abstract text is available yet for this article.
September 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28827099/4d-cardiac-imaging-at-clinical-3-0t-provides-accurate-assessment-of-murine-myocardial-function-and-viability
#6
Lindsey A Crowe, Fabrizio Montecucco, Federico Carbone, Iris Friedli, Anne-Lise Hachulla, Vincent Braunersreuther, François Mach, Jean-Paul Vallée
OBJECTIVES: We validate a 4D strategy tailored for 3T clinical systems to simultaneously quantify function and infarct size in wild type mice after ischemia/reperfusion, with improved spatial and temporal resolution by comparison to previous published protocols using clinical field MRI systems. METHODS: C57BL/6J mice underwent 60min ischemia/reperfusion (n=14) or were controls without surgery (n=6). Twenty-four hours after surgery mice were imaged with gadolinium injection and sacrificed for post-mortem MRI and histology with serum also taken for Troponin I levels...
August 5, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28826917/missense-mutation-in-the-itpr1-gene-presenting-with-ataxic-cerebral-palsy-description-of-an-affected-family-and-literature-review
#7
Joyutpal Das, James Lilleker, Hannah Shereef, John Ealing
The inositol 1,4,5-triphosphate receptor type 1 (ITPR1) gene on chromosome 3 belongs to a family of genes encoding intracellular calcium channel proteins. Such channels are located primarily within the endoplasmic reticular membrane and release Ca(2+), an intracellular messenger, which governs numerous intracellular and extracellular functions. We report a family with infantile-onset cerebellar ataxia with delayed motor development and intellectual disability caused by a heterozygous c.805C>T, p.Arg269Trp missense mutation in ITPR1...
July 8, 2017: Neurologia i Neurochirurgia Polska
https://www.readbyqxmd.com/read/28826607/a-strategy-to-find-suitable-reference-genes-for-mirna-quantitative-pcr-analysis-and-its-application-to-cervical-specimens
#8
Iris Babion, Barbara C Snoek, Mark A van de Wiel, Saskia M Wilting, Renske D M Steenbergen
miRNAs represent an emerging class of promising biomarkers for cancer diagnostics. To perform reliable miRNA expression analysis using quantitative PCR, adequate data normalization is essential to remove nonbiological, technical variations. Ideal reference genes should be biologically stable and reduce technical variability of miRNA expression analysis. Herein is a new strategy for the identification and evaluation of reference genes that can be applied for miRNA-based diagnostic tests without entailing excessive additional experiments...
September 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28826206/isolation-of-an-antiaromatic-singlet-cyclopentadienyl-zwitterion
#9
Paolo Costa, Iris Trosien, Joel Mieres-Pérez, Wolfram Sander
The reaction of triplet tetrachlorocyclopentadienylidene with BF3 in rare gas matrices yields a zwitterion consisting of a cyclopentadienyl cation bearing the positive charge and a negatively charged BF3 unit. IR and UV-vis spectra as well as the absence of EPR signals demonstrate a singlet ground state of the zwitterion, and its calculated geometry and magnetic properties clearly reveal a strong antiaromatic character. The zwitterion is highly labile, and by visible or IR irradiation rearranges via a 1,2-fluorine migration from boron to carbon...
August 22, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28822760/anterior-chamber-perfusion-versus-posterior-chamber-perfusion-does-not-influence-measurement-of-aqueous-outflow-facility-in-living-mice-by-constant-flow-infusion
#10
Navita N Lopez, Gaurang C Patel, Urmimala Raychaudhuri, Subhash Aryal, Tien N Phan, Abbot F Clark, J Cameron Millar
Mice are now routinely utilized in studies of aqueous humor outflow dynamics. In particular, conventional aqueous outflow facility (C) is routinely measured via perfusion of the aqueous chamber by a number of laboratories. However, in mouse eyes perfused ex-vivo, values for C are variable depending upon whether the perfusate is introduced into the posterior chamber (PC) versus the anterior chamber (AC). Perfusion via the AC leads to posterior bowing of the iris, and traction on the iris root/scleral spur, which may increase C...
August 16, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28822300/drinking-water-to-reduce-alcohol-craving-a-randomized-controlled-study-on-the-impact-of-ghrelin-in-mediating-the-effects-of-forced-water-intake-in-alcohol-addiction
#11
Anne Koopmann, Katharina Lippmann, Rilana Schuster, Iris Reinhard, Patrick Bach, Georg Weil, Marcella Rietschel, Stephanie H Witt, Klaus Wiedemann, Falk Kiefer
BACKGROUND: Recent data suggest that ghrelin is involved in the pathophysiology of alcohol use disorders, affecting alcohol self-administration and craving. Gastric ghrelin secretion is reduced by stomach distension. We now tested the hypothesis whether the clinically well-known effects of high-volume water intake on craving reduction in alcoholism is mediated by acute changes in ghrelin secretion. METHODS: In this randomized human laboratory study, we included 23 alcohol-dependent male inpatient subjects who underwent alcohol cue exposure...
August 5, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28822126/dimethyl-labeling-based-quantification-of-the-lysine-acetylome-and-proteome-of-plants
#12
Ines Lassowskat, Markus Hartl, Fabian Hosp, Paul J Boersema, Matthias Mann, Iris Finkemeier
Photorespiratory enzymes in different cellular compartments have been reported to be posttranslational modified by phosphorylation, disulfide formation, S-nitrosylation, glutathionylation, and lysine acetylation. However, not much is known yet about the function of these modifications to regulate the activities, localizations, or interactions of the proteins in this metabolic pathway. Hence, it will be of great importance to study these modifications and their temporal and conditional occurrence in more detail...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28820685/immune-reconstitution-inflammatory-syndrome-in-hiv-infected-immigrants
#13
María Pérez-Rueda, Michele Hernández-Cabrera, Adela Francés-Urmeneta, Alfonso Angel-Moreno, Elena Pisos-Álamo, Nieves Jaén-Sánchez, Cristina Carranza-Rodríguez, Jose-Luis Pérez-Arellano
Immune reconstitution inflammatory syndrome (IRIS) includes a group of potentially serious inflammatory processes that may be present in HIV-infected patients after initiating highly active antiretroviral therapy (HAART). Paradoxical IRIS is a worsening of symptoms, after an overwhelming response to a previously diagnosed opportunistic infection (OI); unmasking IRIS reveals a previously occult OI. The main objective of the study was to describe the epidemiological, clinical, and outcome data of HIV-infected immigrants, stratified according to high- or low-income countries of origin, who developed IRIS and to compare them with native-born Spanish patients...
August 14, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28819785/assessment-of-the-safety-and-efficacy-of-primary-retropupillary-fixation-of-iris-claw-intraocular-lenses-in-children-with-large-lens-subluxations
#14
Anju Rastogi, Apurva Goray, Prolima Thacker, Kamlesh, Babita
PURPOSE: To evaluate whether retropupillary fixation of the iris-claw intraocular lens (IOL) is a safe and effective treatment option in children with large lens subluxations. METHODS: Fourteen eyes of children between the ages of 8-17 years with lens subluxations more than 7 clock hours underwent pars plana lensectomy-vitrectomy with implantation of the iris-claw IOL in the retropupillary position as a primary procedure. The best corrected visual acuity (BCVA), intraocular pressure (IOP), corneal endothelial count (EC) and the lens position using ultrasound biomicroscopy (UBM) were assessed pre- and postoperatively...
August 17, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28819174/interleukin-17-receptor-a-il-17ra-as-a-central-regulator-of-the-protective-immune-response-against-giardia
#15
Oonagh Paerewijck, Brecht Maertens, Leentje Dreesen, Frederik Van Meulder, Iris Peelaers, Dariusz Ratman, Robert W Li, Erik Lubberts, Karolien De Bosscher, Peter Geldhof
The protozoan parasite Giardia is a highly prevalent intestinal pathogen with a wide host range. Data obtained in mice, cattle and humans revealed the importance of IL-17A in the development of a protective immune response against Giardia. The aim of this study was to further unravel the protective effector mechanisms triggered by IL-17A following G. muris infection in mice, by an RNA-sequencing approach. C57BL/6 WT and C57BL/6 IL-17RA KO mice were orally infected with G. muris cysts. Three weeks post infection, intestinal tissue samples were collected for RNA-sequencing, with samples from uninfected C57BL/6 WT and C57BL/6 IL-17RA KO animals serving as negative controls...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818665/irf-5-accelerates-leukocyte-adhesion-to-endothelial-cells-in-ischemia-reperfusion-injury-through-regulating-the-transcription-of-vcam-1
#16
Hongbin Cai, Zhuhua Yao, Wenting Li
Ischemia-reperfusion injury (IRI) has been implicated in many pathological conditions, including cardiovascular diseases. Adhesion of leukocytes to the surface of endothelial cells has been considered as one of the principle steps in the pathological cascade of inflammatory tissue damage during IRI. The role of the transcriptional factor interferon regulatory factor-5 (IRF-5) in endothelial physiology remains unknown. Here, we report that IRF-5 is expressed in human umbilical vein endothelial cells (HUVECs) and is rapidly upregulated in response to IRI, mediated by the JAK2/STAT3 pathway...
August 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28817953/the-evidence-base-for-interventions-targeting-individuals-with-work-related-ptsd-a-systematic-review-and-recommendations
#17
Iris Torchalla, Verena Strehlau
The purpose of this study was to summarize the evidence base for interventions targeting individuals with work-related posttraumatic stress disorder (PTSD), to make recommendations for clinicians and administrative decision makers involved in their rehabilitation, and to guide future research in this area. Particular attention was given to studies that were conducted in naturalistic clinical settings or in a workers' compensation claim context. Electronic searches of Cochrane Central Register of Controlled Trials, MEDLINE, PubMed, PsycINFO, CINAHL, PILOTS, and EMBASE identified 11 articles...
August 1, 2017: Behavior Modification
https://www.readbyqxmd.com/read/28817736/performance-of-class-i-composite-restorations-when-polished-immediately-or-after-one-day-water-storage
#18
Masao Irie, Yukinori Maruo, Goro Nishigawa
This study investigated the effects on gap formation in Class I restorations (observed by vertical and horizontal forms of inspection) and on the mechanical properties of nine resin composite filling materials when the restorations were subject to finishing immediately after setting or after one-day water storage. Class I restorations with resin composite fillings were polished either immediately (3 min) after setting or after one-day water storage. Interfacial gap formation (observed by vertical inspection) was assessed using 14 gap measurement points along the interface between the restoration and cavity walls and floor (n = 10 per resin composite; total points measured per time point = 140)...
2017: PloS One
https://www.readbyqxmd.com/read/28817187/second-line-treatment-in-patients-with-pancreatic-ductal-adenocarcinoma-a-meta-analysis
#19
Mohamad Bassam Sonbol, Belal Firwana, Zhen Wang, Diana Almader-Douglas, Mitesh J Borad, Issam Makhoul, Ramesh K Ramanathan, Daniel H Ahn, Tanios Bekaii-Saab
BACKGROUND: There are limited therapeutic options for treatment-refractory pancreatic ductal adenocarcinoma (PDAC), with a paucity of data to support the best option after progression on gemcitabine-based regimens. The authors performed a meta-analysis to determine the effectiveness of adding oxaliplatin (OX) or various irinotecan formulations to a fluoropyrimidine (FP) after first-line treatment progression in patients with PDAC. METHODS: Different databases, including PubMed, EMBASE, and Cochrane, were searched to identify randomized controlled trials comparing FP monotherapy versus FP combination therapy that included either oxaliplatin (FPOX) or various irinotecan formulations (FPIRI) in patients with PDAC who progressed after first-line treatment...
August 17, 2017: Cancer
https://www.readbyqxmd.com/read/28817074/familial-resemblance-in-dietary-intakes-of-children-adolescents-and-parents-does-dietary-quality-play-a-role
#20
Leonie H Bogl, Karri Silventoinen, Antje Hebestreit, Timm Intemann, Garrath Williams, Nathalie Michels, Dénes Molnár, Angie S Page, Valeria Pala, Stalo Papoutsou, Iris Pigeot, Lucia A Reisch, Paola Russo, Toomas Veidebaum, Luis A Moreno, Lauren Lissner, Jaakko Kaprio
Information on familial resemblance is important for the design of effective family-based interventions. We aimed to quantify familial correlations and estimate the proportion of variation attributable to genetic and shared environmental effects (i.e., familiality) for dietary intake variables and determine whether they vary by generation, sex, dietary quality, or by the age of the children. The study sample consisted of 1435 families (1007 mothers, 438 fathers, 1035 daughters, and 1080 sons) from the multi-center I...
August 17, 2017: Nutrients
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