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https://www.readbyqxmd.com/read/28218804/detection-of-lunasin-in-quinoa-chenopodium-quinoa-willd-and-the-in-vitro-evaluation-of-its-antioxidant-and-anti-inflammatory-activities
#1
Guixing Ren, Yingying Zhu, Zhenxing Shi
BACKGROUND: Lunasin is a novel cancer preventive peptide that has been detected in various plants. However, the presence and bioactivity of lunasin in quinoa has not been demonstrated. RESULTS: Lunasin was detected in quinoa using UPLC-ESI-MS. The content in fifteen quinoa samples ranged from 1.01 × 10(-3) g kg(-1) dry seed to 4.89 × 10(-3) g kg(-1) dry seed. Significant differences (p<0.05) in lunasin content among different cultivars (yy22, xsg86, xsg59, yy30 and yy19) from the same area and the same cultivar from different areas (Hainan, Hebei and Shanxi provinces) were observed...
February 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28182687/lunasin-attenuates-obesity-related-inflammation-in-raw264-7-cells-and-3t3-l1-adipocytes-by-inhibiting-inflammatory-cytokine-production
#2
Chia-Chien Hsieh, Mei-Jia Chou, Chih-Hsuan Wang
Obesity has become a major threat to public health and is accompanied by chronic low-grade inflammation, which leads to various pathological developments. Lunasin, a natural seed peptide, exhibits several biological activities, such as anti-carcinogenesis, anti-inflammatory, and antioxidant activities. However, the mechanism of action of lunasin in obesity-related inflammation has not been investigated. The aim of this study was to explore whether lunasin could reduce the inflammation induced by obesity-related mediators in RAW264...
2017: PloS One
https://www.readbyqxmd.com/read/28151045/hirudin-as-a-novel-fusion-tag-for-efficient-production-of-lunasin-in-escherichia-coli
#3
Qinghua Tian, Ping Zhang, Zhan Gao, Hengli Li, Zhengli Bai, Shuhua Tan
Fusion expression provides an effective means for the biosynthesis of longer peptides in Escherichia coli. However, the commonly used fusion tags are primarily suitable for laboratory scale applications due to the high cost of the commercial affinity resins. Herein, a novel approach exploiting hirudin as a multipurpose fusion tag in combination with TEV protease cleavage has been developed for the efficient and cost-effective production of a 43-amino acids model peptide lunasin in E. coli at preparative scale...
February 2, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27983683/lunasin-attenuates-obesity-associated-metastasis-of-4t1-breast-cancer-cell-through-anti-inflammatory-property
#4
Chia-Chien Hsieh, Chih-Hsuan Wang, Yu-Shan Huang
Obesity prevalence is increasing worldwide and is accompanied by low-grade inflammation with macrophage infiltration, which is linked with a poorer breast cancer prognosis. Lunasin is a natural seed peptide with chemopreventive properties and multiple bioactivities. This is the first study to explore the chemopreventive effects of lunasin in the obesity-related breast cancer condition using 4T1 breast cancer cells, 3T3-L1 adipocytes, and conditioned media. An obesity-related environment, such as leptin-treatment or adipocyte-conditioned medium (Ad-CM), promoted 4T1 cell proliferation and metastasis...
December 15, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27639324/lunasin-is-a-redox-sensitive-intrinsically-disordered-peptide-with-two-transiently-populated-%C3%AE-helical-regions
#5
Rihards Aleksis, Kristaps Jaudzems, Ruta Muceniece, Edvards Liepinsh
Lunasin is a 43 amino acid peptide with anti-cancer, antioxidant, anti-inflammatory and cholesterol-lowering properties. Although the mechanism of action of lunasin has been characterized to some extent, its exact three-dimensional structure as well as the function of the N-terminal sequence remains unknown. We established a novel method for the production of recombinant lunasin that allows efficient isotope labeling for NMR studies. Initial studies showed that lunasin can exist in a reduced or oxidized state with an intramolecular disulfide bond depending on solution conditions...
September 14, 2016: Peptides
https://www.readbyqxmd.com/read/27566591/lunasin-is-a-novel-therapeutic-agent-for-targeting-melanoma-cancer-stem-cells
#6
Chris Shidal, Numan Al-Rayyan, Kavitha Yaddanapudi, Keith R Davis
Recent studies provide compelling evidence that melanoma is initiated and maintained by a small population of malignant cells called cancer-initiating cells (CICs) that exhibit stem-cell-like properties. Observations that CICs have a distinct biology when compared to that of the bulk tumor cells and, importantly, are resistant to chemotherapies and radiation, suggest that CICs are involved in invasion, metastasis, and ultimately relapse. Lunasin, a bioactive peptide present in soybean, has both chemopreventive activity and chemotherapeutic activity against multiple cancer types...
August 23, 2016: Oncotarget
https://www.readbyqxmd.com/read/27189269/commercial-processed-soy-based-food-product-contains-glycated-and-glycoxidated-lunasin-proteoforms
#7
Aida Serra, Xavier Gallart-Palau, Rachel Su-En See-Toh, Xinya Hemu, James P Tam, Siu Kwan Sze
Nutraceuticals have been proposed to exert positive effects on human health and confer protection against many chronic diseases. A major bioactive component of soy-based foods is lunasin peptide, which has potential to exert a major impact on the health of human consumers worldwide, but the biochemical features of dietary lunasin still remain poorly characterized. In this study, lunasin was purified from a soy-based food product via strong anion exchange solid phase extraction and then subjected to top-down mass spectrometry analysis that revealed in detail the molecular diversity of lunasin in processed soybean foods...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27175819/lunasin-suppresses-the-migration-and-invasion-of-breast-cancer-cells-by-inhibiting-matrix-metalloproteinase-2-9-via-the-fak-akt-erk-and-nf-%C3%AE%C2%BAb-signaling-pathways
#8
Qianqian Jiang, Yu Pan, Yupeng Cheng, Huiling Li, Dandan Liu, Hui Li
Lunasin is a naturally existing bioactive peptide with an Arg-Gly-Asp (RGD) motif, which competes with integrins to bind with the extracellular matrix (ECM) consequently suppressing the integrin-mediated signaling pathway. Owing to the RGD motif, lunasin has been proven as an effective anti-inflammatory, antitumor and antimetastatic agent in many types of cancer. However, knowledge of its inhibitory effect on metastasis and the related mechanism of action in breast cancer cells is limited. In this study, the inhibitory effect of lunasin on the proliferation, migration and invasion of two typical breast cancer cell lines, ER-negative MDA-MB-231 with αVβ3 expression and ER-positive MCF-7 with αVβ5/α5β1 expression, were examined in vitro as well the related mechanisms...
July 2016: Oncology Reports
https://www.readbyqxmd.com/read/27145928/the-chemopreventive-peptide-lunasin-inhibits-d-galactose-induced-experimental-cataract-in-rats
#9
Guangzhi Dai, Ping Zhang, Pei Ye, Miaoqing Zhang, Ning Han, Haoyue Shuai, Shuhua Tan
Oxidative damage to the constituents of the eye lens is a major mechanism in the initiation and development of cataract. Lunasin, a 43-amino acids chemoprevention peptide, has been proved to possess potent anti-oxidative activity other than its established anticancer activities. Herein, we explored whether lunasin has preventative effects on d-galactose-induced experimental cataract in rat. After modeling, SD rats were administrated by instillation, 80 µM of lunasin eye drops to each eye thrice daily and consecutively for 30 days...
2016: Protein and Peptide Letters
https://www.readbyqxmd.com/read/27109319/pharmacological-research-on-natural-substances-in-latvia-focus-on-lunasin-betulin-polyprenol-and-phlorizin
#10
REVIEW
Ruta Muceniece, Jana Namniece, Ilva Nakurte, Kaspars Jekabsons, Una Riekstina, Baiba Jansone
In this concise review the current research in plant bioactive compound studies in Latvia is described. The paper summarizes recent studies on substances from edible plants (e.g., cereals and apples) or their synthetic analogues, such as peptide lunasin, as well as substances isolated from inedible plants (e.g., birch and conifer), such as pentacyclic triterpenes (e.g., betulin, betulinic acid, and lupeol) and polyprenols. Latvian researchers have been first to demonstrate the presence of lunasin in triticale and oats...
November 2016: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/27015324/immunological-investigation-for-the-presence-of-lunasin-a-chemopreventive-soybean-peptide-in-the-seeds-of-diverse-plants
#11
Alaa A Alaswad, Hari B Krishnan
Lunasin, a 44 amino acid soybean bioactive peptide, exhibits anticancer and anti-inflammatory properties. All soybean varieties that have been examined contain lunasin. It has also been reported in a few other plant species including amaranth, black nightshade, wheat, barley, rye, and triticale. Interestingly, detailed searches of transcriptome and DNA sequence databases of cereals failed to identify lunasin-coding sequences, raising questions about the authenticity of lunasin in cereals. To clarify the presence or absence of lunasin in cereals and other plant species, an immunological investigation was conducted utilizing polyclonal antibodies raised against the first 20 amino acid N-terminal peptide (SKWQHQQDSCRKQLQGVNLT) and a 15 amino acid C-terminal peptide (CEKHIMEKIQGRGDD) of lunasin...
April 13, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/26506839/metformin-and-soybean-derived-bioactive-molecules-attenuate-the-expansion-of-stem-cell-like-epithelial-subpopulation-and-confer-apoptotic-sensitivity-in-human-colon-cancer-cells
#12
Maria Theresa E Montales, Rosalia C M Simmen, Ederlan S Ferreira, Valdir A Neves, Frank A Simmen
Colorectal cancer (CRC) is a disease whose genesis may include metabolic dysregulation. Cancer stem cells are attractive targets for therapeutic interventions since their aberrant expansion may underlie tumor initiation, progression, and recurrence. To investigate the actions of metabolic regulators on cancer stem cell-like cells (CSC) in CRC, we determined the effects of soybean-derived bioactive molecules and the anti-diabetes drug metformin (MET), alone and together, on the growth, survival, and frequency of CSC in human HCT116 cells...
November 2015: Genes & Nutrition
https://www.readbyqxmd.com/read/26494432/italian-legumes-effect-of-sourdough-fermentation-on-lunasin-like-polypeptides
#13
Carlo Giuseppe Rizzello, Blanca Hernández-Ledesma, Samuel Fernández-Tomé, José Antonio Curiel, Daniela Pinto, Barbara Marzani, Rossana Coda, Marco Gobbetti
BACKGROUND: There is an increasing interest toward the use of legumes in food industry, mainly due to the quality of their protein fraction. Many legumes are cultivated and consumed around the world, but few data is available regarding the chemical or technological characteristics, and especially on their suitability to be fermented. Nevertheless, sourdough fermentation with selected lactic acid bacteria has been recognized as the most efficient tool to improve some nutritional and functional properties...
2015: Microbial Cell Factories
https://www.readbyqxmd.com/read/26132418/the-protective-role-of-the-bowman-birk-protease-inhibitor-in-soybean-lunasin-digestion-the-effect-of-released-peptides-on-colon-cancer-growth
#14
Elvia Cruz-Huerta, Samuel Fernández-Tomé, M Carmen Arques, Lourdes Amigo, Isidra Recio, Alfonso Clemente, Blanca Hernández-Ledesma
Lunasin is a naturally-occurring peptide demonstrating chemopreventive, antioxidant and anti-inflammatory properties. To exhibit these activities, orally ingested lunasin needs to survive proteolytic attack of digestive enzymes to reach target tissues in active form/s. Preliminary studies suggested the protective role of protease inhibitors, such as the Bowman-Birk inhibitor and Kunitz-trypsin inhibitor, against lunasin's digestion by both pepsin and pancreatin. This work describes in depth the behaviour of lunasin under conditions simulating the transit through the gastrointestinal tract in the absence or presence of soybean Bowman-Birk isoinhibitor 1 (IBB1) in both active and inactive states...
August 2015: Food & Function
https://www.readbyqxmd.com/read/25692134/lunasin-inhibits-cell-proliferation-via-apoptosis-and-reduces-the-production-of-proinflammatory-cytokines-in-cultured-rheumatoid-arthritis-synovial-fibroblasts
#15
Shaohui Jia, Shufang Zhang, Hong Yuan, Ning Chen
Lunasin, a peptide with 43 amino acid residues and initially isolated and identified in soybean cotyledon, has gained extensive attention due to its anti-inflammatory and anticancer properties. However, its treatment efficacy on rheumatoid arthritis (RA) and corresponding mechanisms have not been reported. Herein, the synovial fibroblasts harvested and isolated from patients with RA were treated with lunasin at various concentrations to examine the proliferation, apoptosis status, and corresponding cell cycle of cultured RA synovial fibroblasts...
2015: BioMed Research International
https://www.readbyqxmd.com/read/25660866/an-effective-and-simple-procedure-to-isolate-abundant-quantities-of-biologically-active-chemopreventive-lunasin-protease-inhibitor-concentrate-lpic-from-soybean
#16
Hari B Krishnan, Thomas T Y Wang
Lunasin is a 5-kDa soybean bioactive peptide with demonstrated anti-cancer and anti-inflammatory properties. Recently, purification methods have been developed to obtain gram quantities of lunasin. However, these methods are cumbersome, time consuming and cost-prohibitive. To overcome these constrains we have developed a novel method which involves extraction of soybean flour with 30% ethanol followed by preferential precipitation of lunasin and protease inhibitors by calcium. The calcium precipitated protein fraction, which we termed as Lunasin Protease Inhibitor Concentrate (LPIC), contains three abundant proteins with molecular weights of 21, 14 and 5 kDa...
June 15, 2015: Food Chemistry
https://www.readbyqxmd.com/read/25646897/lunasin-alleviates-allergic-airway-inflammation-while-increases-antigen-specific-tregs
#17
Xiaowei Yang, Jingjing Zhu, Chun-Yu Tung, Gail Gardiner, Qun Wang, Hua-Chen Chang, Baohua Zhou
Lunasin is a naturally occurring peptide isolated from soybeans and has been explored in cancer treatment. Lunasin inhibits NF-κB activation and thus pro-inflammatory cytokine and mediator production in macrophages. In this study we demonstrate that lunasin can effectively suppress allergic airway inflammation in two murine models of asthma. In an OVA+Alum sensitization model, intranasal lunasin treatment at the time of OVA challenges significantly reduced total cells counts in bronchoalveolar lavage (BAL) fluid and eosinophilia, peribronchiolar inflammatory infiltration, goblet cell metaplasia and airway IL-4 production...
2015: PloS One
https://www.readbyqxmd.com/read/25627564/-potential-health-benefits-of-lunasin-a-multifaceted-soy-derived-bioactive-peptide
#18
REVIEW
Vaibhao Kisanrao Lule, Sheenam Garg, Sarang Dilip Pophaly, Hitesh, Sudhir Kumar Tomar
Bioactive peptides are small protein fragments derived from enzymatic hydrolysis of food proteins, fermentation with proteolytic starter cultures, and gastrointestinal digestion. These peptides have positive impacts on a number of physiological functions in living beings. Lunasin, a soy-derived bioactive peptide, is one of the most promising among them. Lunasin encoded within 2S albumin (GM2S-1) gene, identified as a novel peptide extracted from soybean seed. It is composed of 43 amino acid residues with a molecular weight of 5...
March 2015: Journal of Food Science
https://www.readbyqxmd.com/read/25609198/the-soybean-derived-peptide-lunasin-inhibits-non-small-cell-lung-cancer-cell-proliferation-by-suppressing-phosphorylation-of-the-retinoblastoma-protein
#19
Elizabeth J McConnell, Bharat Devapatla, Kavitha Yaddanapudi, Keith R Davis
Lunasin, a soybean bioactive peptide, has both chemopreventive and chemotherapeutic activities. The aim of this study was to determine the chemotherapeutic potential of lunasin against human lung cancer. Treatment of non-small cell lung cancer (NSCLC) cells with highly purified soybean-derived lunasin caused limited, cell-line specific anti-proliferative effects on anchorage-dependent growth whereas two normal bronchial epithelial cell lines were unaffected. Lunasin's antiproliferative effects were potentiated upon utilization of anchorage-independent conditions...
March 10, 2015: Oncotarget
https://www.readbyqxmd.com/read/25530619/lunasin-sensitivity-in-non-small-cell-lung-cancer-cells-is-linked-to-suppression-of-integrin-signaling-and-changes-in-histone-acetylation
#20
Junichi Inaba, Elizabeth J McConnell, Keith R Davis
Lunasin is a plant derived bioactive peptide with both cancer chemopreventive and therapeutic activity. We recently showed lunasin inhibits non-small cell lung cancer (NSCLC) cell proliferation in a cell-line-specific manner. We now compared the effects of lunasin treatment of lunasin-sensitive (H661) and lunasin-insensitive (H1299) NSCLC cells with respect to lunasin uptake, histone acetylation and integrin signaling. Both cell lines exhibited changes in histone acetylation, with H661 cells showing a unique increase in H4K16 acetylation...
2014: International Journal of Molecular Sciences
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