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https://www.readbyqxmd.com/read/28644779/cellular-light-scattering-for-the-identification-of-bacteria-and-its-application-to-the-identification-of-staphylococcus
#1
David L Haavig, Kaylagh R Hollen, Abbey E Debruin, Shelbi R Lisecki, Nicole C Shoup, Mickey J H Stimac, Anthony M Treloar, Zachary N Jodoin, Margaret S Bohm, Josh S Sharp
Rapid identification of bacteria is critical in clinical and food safety applications. This paper describes anovel instrument and data analysis method for identifying bacteria based on the measurement of laser light scattering as the beam interacts with bacterial cells suspended in water. A description of the technology is followed by an identification performance study for a set of strains from the genus Staphylococcus (the inclusive target organisms) and a set of non-Staphylococcus strains (the exclusive organisms)...
June 23, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28643898/identification-of-cross-reactive-b-cell-epitopes-between-bos-d-9-0101-bos-taurus-and-gly-m-5-0101-glycine-max-by-epitope-mapping-maldi-tof-ms
#2
Ángela María Candreva, Mario Ferrer-Navarro, Silvia Bronsoms, Alejandra Quiroga, Renata Curciarello, Ana Cauerhff, Silvana Petruccelli, Guillermo Horacio Docena, Sebastián Alejandro Trejo
Exposure to cow's milk constitutes one of the most common causes of food allergy. In addition, exposure to soy proteins has become relevant in a restricted proportion of milk allergic pediatric patients treated with soy formulae as a dairy substitute, because of the cross-allergenicity described between soy and milk proteins . We have previously identified several cross-reactive allergens between milk and soy that may explain this intolerance. The purpose of the present work was to identify epitopes in the purified αS1-casein and the recombinant soy allergen Gly m 5...
June 23, 2017: Proteomics
https://www.readbyqxmd.com/read/28642459/meta-analysis-of-soy-consumption-and-gastrointestinal-cancer-risk
#3
Demin Lu, Chi Pan, Chenyang Ye, Huijie Duan, Fei Xu, Li Yin, Wei Tian, Suzhan Zhang
Soy consumption has received considerable attention for its potential role in reducing cancer incidence and mortality. However, its effects on gastrointestinal (GI) cancer are controversial. Therefore, we performed a meta-analysis to evaluate the association between soy consumption and gastrointestinal cancer risk by searching for prospective studies in PubMed, Web of Science, EMBASE and the reference lists of the included articles. The study-specific odds ratio (OR), relative risk (RR) or hazard ratio (HR) estimates and 95% confidence intervals (CIs) were pooled using either a fixed-effect or random-effect model...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28638093/fatty-acid-composition-and-phospholipid-types-used-in-infant-formulas-modifies-the-establishment-of-human-gut-bacteria-in-germ-free-mice
#4
Rikke Guldhammer Nejrup, Tine Rask Licht, Lars Ingvar Hellgren
Human milk fat contains high concentrations of medium-chained fatty acids (MCFA) and triacylglycerols emulsified by a sphingomyelin-rich phospholipid membrane (milk phospholipids, MPL). Infant formula comprises mainly long-chained fatty acids (LCFA) emulsified with dairy proteins and soy lecithin (SL) lacking sphingomyelin. Sphingomyelin content and saturation level of phospholipids affect the gut lipase activity, which alters the concentrations of lipid hydrolysis products in ileum and colon, and hereby putatively affects the competitive advantage of specific gut bacteria...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28637300/commercial-rodent-diets-differentially-regulate-autoimmune-glomerulonephritis-epigenetics-and-microbiota-in-mrl-lpr-mice
#5
Michael R Edwards, Rujuan Dai, Bettina Heid, Thomas E Cecere, Deena Khan, Qinghui Mu, Catharine Cowan, Xin M Luo, S Ansar Ahmed
The course and severity of lupus in spontaneous murine lupus models varies among laboratories, which may be due to variations in diet, housing and/or local environmental conditions. In this study, we investigated the influence of common rodent diets while keeping other factors constant. Female lupus-prone MRL/lpr mice were subjected to the same housing conditions and given one of the three diets: Teklad 7013 containing isoflavone rich soy and alfalfa, Harlan 2018 isoflavone rich soy-based diet, or ResearchDiets Inc...
June 15, 2017: International Immunology
https://www.readbyqxmd.com/read/28633868/daidzein-stimulates-osteogenesis-facilitating-proliferation-differentiation-and-antiapoptosis-in-human-osteoblast-like-mg-63-cells-via-estrogen-receptor-dependent-mek-erk-and-pi3k-akt-activation
#6
Xin Jin, Jing Sun, Bo Yu, Yue Wang, Wei Jia Sun, Jing Yang, Su Hui Huang, Wen Li Xie
Daidzein, a natural soy isoflavone, has a structure similar to estradiol and exhibiting bone-sparing effects against osteoporosis. However, the molecular mechanisms of osteogenesis remain unclear. We hypothesized that daidzein stimulates osteogenesis through estrogen receptor (ER)-dependent signal pathways. To test this hypothesis, we investigated the effects of daidzein compared with 17β-estradiol on proliferation, differentiation, and cisplatin-induced apoptosis in human osteoblast-like MG-63 cells containing 2 ER isoforms...
June 2017: Nutrition Research
https://www.readbyqxmd.com/read/28632934/a-prospective-microbiome-wide-association-study-of-food-sensitization-and-food-allergy-in-early-childhood
#7
Jessica H Savage, Kathleen A Lee-Sarwar, Joanne Sordillo, Supinda Bunyavanich, Yanjiao Zhou, George O'Connor, Megan Sandel, Leonard Bacharier, Robert Zeiger, Erica Sodergren, George M Weinstock, Diane R Gold, Scott T Weiss, Augusto A Litonjua
BACKGROUND: Alterations in the intestinal microbiome are prospectively associated with the development of asthma; less is known regarding the role of microbiome alterations in food allergy development. METHODS: Intestinal microbiome samples were collected at age 3-6 months in children participating in the follow-up phase of an interventional trial of high dose Vitamin D given during pregnancy. At age 3, sensitization to foods (milk, egg, peanut, soy, wheat, walnut) was assessed...
June 20, 2017: Allergy
https://www.readbyqxmd.com/read/28632187/complex-coacervation-of-soy-proteins-isoflavones-and-chitosan
#8
Yu-Hsuan Hsiao, Sheng-Yang Hsia, Yin-Ching Chan, Jung-Feng Hsieh
In this study, the chitosan-induced coacervation of soy protein-isoflavone complexes in soymilk was investigated. Most of the soymilk proteins, including β-conglycinin (7S), glycinin (11S), and isoflavones, were found to coacervate into the soymilk pellet fraction (SPF) following the addition of 0.5% chitosan. The total protein in the soymilk supernatant fraction (SSF) decreased from 18.1 ± 0.3 mg/mL to 1.6 ± 0.1 mg/mL, and the pH values decreased slightly, from 6.6 ± 0.0 to 6.0 ± 0.0. The results of SDS-PAGE revealed that the 7S α', 7S α, 7S β, 11S A3, and 11S acidic subunits, as well as the 11S basic proteins in the SSF, decreased to 0...
June 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28630857/effectiveness-of-a-commercial-lactic-acid-bacteria-intervention-applied-to-inhibit-shiga-toxin-producing-escherichia-coli-on-refrigerated-vacuum-aged-beef
#9
Katie R Kirsch, Tamra N Tolen, Jessica C Hudson, Alejandro Castillo, Davey Griffin, T Matthew Taylor
Because of their antagonistic activity towards pathogenic and spoilage bacteria, some members of the lactic acid bacteria (LAB) have been evaluated for use as food biopreservatives. The objectives of this study were to assess the antimicrobial utility of a commercial LAB intervention against O157 and non-O157 Shiga-toxigenic E. coli (STEC) on intact beef strip loins during refrigerated vacuum aging and determine intervention efficacy as a function of mode of intervention application. Prerigor strip loins were inoculated with a cocktail (8...
2017: International journal of food science
https://www.readbyqxmd.com/read/28629639/atp-synthesis-capacity-of-pulsed-light-exposed-bacteria
#10
B Kramer, J Wunderlich, P Muranyi
The ability of four different bacteria to synthesize new ATP upon exposure to different doses of pulsed-light (PL) irradiation was investigated. The bacterial cells were PL treated on a gel surface, resuspended in phosphate buffered saline (PBS) and subsequently incubated in Tryptic Soy Broth (TSB) at 37°C. Cellular ATP levels were monitored during a 2h incubation period and compared to the respective colony count data. Although PL affected ATP production in a dose dependent manner, the results showed that bacteria, which had rendered unculturable after PL exposure, are still capable of generating significant quantities of ATP...
June 7, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28627579/correlation-analysis-of-protein-quality-characteristics-with-gluten-free-bread-properties
#11
S W Horstmann, M Foschia, E K Arendt
The interest in gluten-free cereal products has increased significantly over the last number of years and there is still a high demand for high quality products. This study aims to establish possible connections between protein properties and dough and bread quality which could advance the knowledge for gluten-free product development. The objective of the present study was to correlate protein properties with bread characteristics. Therefore, a wide range of tests (solubility, emulsifying, foaming, water hydration properties) was performed to characterize a range of food proteins (potato, pea, carob, lupin and soy)...
June 19, 2017: Food & Function
https://www.readbyqxmd.com/read/28627453/design-and-development-of-multi-walled-carbon-nanotube-liposome-drug-delivery-platforms
#12
Natassa Pippa, Demetrios D Chronopoulos, Dimitris Stellas, Rodrigo Fernández-Pacheco, Raul Arenal, Costas Demetzos, Nikos Tagmatarchis
The aim of this study is to design and develop delivery platforms made of liposomes and multi-walled carbon nanotubes (MWCNTs). We used different lipids with different main transition temperature (Tm) and differently functionalized MWCNTs with organic addends possessing either positive or negative charge. The phospholipids used for the formulations were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) (Tm=41°C) and L-α-phosphatidylcholine, hydrogenated Soy (HSPC) (Tm=53°C). By Differential Scanning Calorimetry (DSC), we studied the interaction between the DPPC and HSPC bilayers and MWCNTs...
June 13, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28624912/sustainable-cattle-ranching-in-practice-moving-from-theory-to-planning-in-colombia-s-livestock-sector
#13
Amy M Lerner, Andrés Felipe Zuluaga, Julián Chará, Andrés Etter, Timothy Searchinger
A growing population with increasing consumption of milk and dairy require more agricultural output in the coming years, which potentially competes with forests and other natural habitats. This issue is particularly salient in the tropics, where deforestation has traditionally generated cattle pastures and other commodity crops such as corn and soy. The purpose of this article is to review the concepts and discussion associated with reconciling food production and conservation, and in particular with regards to cattle production, including the concepts of land-sparing and land-sharing...
June 17, 2017: Environmental Management
https://www.readbyqxmd.com/read/28624078/effect-of-added-ingredients-on-water-status-and-physico-chemical-properties-of-tomato-sauce
#14
Agoura Diantom, Elena Curti, Eleonora Carini, Elena Vittadini
Different ingredients (guar, xanthan, carboxy methyl cellulose, locust bean gums, potato fiber, milk, potato and soy proteins) were added to tomato sauce to investigate their effect on its physico-chemical properties. The products were characterized in terms of colour, rheological properties (Bostwick consistency, flow behavior and consistency coefficient), water status (water activity, moisture content) and molecular mobility by (1)H Nuclear Magnetic Resonance (NMR). Water activity was significantly decreased only by the addition of potato fiber...
December 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28621401/encapsulation-of-sour-cherry-pomace-extract-by-freeze-drying-characterization-and-storage-stability
#15
Vesna Šaponjac, Gordana Ćetković, Jasna Čanadanović-Brunet, Sonja Đilas, Biljana Pajin, Jovana Petrović, Stajčić Slađana, Jelena Vulić
In this study sour cherry pomace was extracted with food-grade solvent (50% ethanol), concentrated and stabilized in whey and soy proteins by encapsulation. Soy proteins exhibited higher encapsulation efficiency (94.90%), but not significantly (p < 0.05), from whey (90.10%). Storage properties of whey (WP) and soy protein (SP) encapsulates in terms of total polyphenols, anthocyanins and antioxidant activity were tested for 6 weeks. At the end of storage period the retention of polyphenols in SP and WP was similar (67...
June 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28619236/the-effect-of-isolated-soy-protein-adjunctive-with-flaxseed-oil-on-markers-of-inflammation-oxidative-stress-acute-phase-proteins-and-wound-healing-of-burn-patients-a-randomized-clinical-trial
#16
Siavash Babajafari, Masoumeh Akhlaghi, Seyed Mohammad Mazloomi, Mehdi Ayaz, Ali Noorafshan, Peyman Jafari, Abdollah Hojhabrimanesh
INTRODUCTION: The objective was to determine the effect of isolated soy protein (ISP) and flaxseed oil (FO) on inflammatory and oxidative stress indices, acute phase proteins, and wound healing of burn patients. METHODS: One hundred eighty-eight patients were assessed for eligibility in this randomized controlled trial. Of these, seventy-three eligible patients (total burn surface area 20-50%) were randomly assigned to 3 isocaloric groups, labeled as control (wheat flour+corn oil (CO)), ISP+FO, and ISP+CO, to receive these nutrients for 3 weeks...
June 12, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28613908/metabolomics-analysis-of-effects-of-commercial-soy-based-protein-products-in-red-drum-sciaenops-ocellatus
#17
Fabio Casu, Aaron M Watson, Justin Yost, John W Leffler, Thomas Gibson Gaylord, Frederic T Barrows, Paul A Sandifer, Michael R Denson, Daniel W Bearden
We investigated the metabolic effects of four different commercial soy-based protein products on red drum fish (Sciaenops ocellatus) using nuclear magnetic resonance (NMR) spectroscopy-based metabolomics along with unsupervised principal component analysis (PCA) to evaluate metabolic profiles in liver, muscle, and plasma tissues. Specifically, during a 12 week feeding trial, juvenile red drum maintained in an indoor recirculating aquaculture system were fed four different commercially available soy formulations, containing the same amount of crude protein, and two reference diets as performance controls: a 60% soybean meal diet that had been used in a previous trial in our lab and a natural diet...
June 21, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28606841/effects-of-adding-corn-oil-and-soy-protein-to-corn-starch-on-the-physicochemical-and-digestive-properties-of-the-starch
#18
Xu Chen, Xiao-Wei He, Bin Zhang, Xiong Fu, Jay-Lin Jane, Qiang Huang
This study aimed to understand effects of adding corn oil (CO) and soy protein (SP) to corn starch on the physicochemical properties and digestive rates of annealed starch complex and mechanisms of interactions between corn starch (CS), CO and SP. Binary and ternary blends were prepared using CS mixed with CO (10%, dsb) and/or SP (10%, dsb) and incubated in a water bath at 50°C for 14h. Results showed that more agglomerates of the granules were in the ternary blends. With the addition of CO and/or SP, the CS displayed a decreased pasting temperature, an increased peak viscosity and a decreased enthalpy change of amylose-lipid complex dissociation...
June 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28606822/genistein-a-dietary-soy-isoflavone-exerts-antidepressant-like-effects-in-mice-involvement-of-serotonergic-system
#19
Pei Hu, Li Ma, Yan-Gui Wang, Feng Ye, Chuang Wang, Wen-Hua Zhou, Xin Zhao
Genistein, a principal isoflavone property of soybeans, possesses multiple pharmacological activities such as neuroprotection. Recently, it was reported that genistein exerted antidepressant-like effects in animal models, but the mechanism of action remains ambiguous. The purpose of this study was to investigate the antidepressant-like effect of genistein in mice and explore the underlying mechanism(s), using two mouse models of depression, i.e. forced swim test (FST) and tail suspension test (TST). Chronic, but not acute (single dose), genistein treatment (5, 15 or 45 mg/kg, p...
June 9, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28605664/soy-compared-with-milk-protein-in-a-western-diet-changes-fecal-microbiota-and-decreases-hepatic-steatosis-in-obese-oletf-rats
#20
Matthew R Panasevich, Colin M Schuster, Kathryn E Phillips, Grace M Meers, Sree V Chintapalli, Umesh D Wankhade, Kartik Shankar, Dustie N Butteiger, Elaine S Krul, John P Thyfault, R Scott Rector
Soy protein is effective at preventing hepatic steatosis; however, the mechanisms are poorly understood. We tested the hypothesis that soy vs. dairy protein-based diet would alter microbiota and attenuate hepatic steatosis in hyperphagic Otsuka Long-Evans Tokushima fatty (OLETF) rats. Male OLETF rats were randomized to "Western" diets containing milk protein isolate (MPI), soy protein isolate (SPI) or 50:50 MPI/SPI (MS) (n=9-10/group; 21% kcal protein) for 16 weeks. SPI attenuated (P<.05) fat mass and percent fat by ~10% compared with MS, but not compared with MPI...
May 31, 2017: Journal of Nutritional Biochemistry
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