Yutong Song, Zhihao Zhao, Linyu Xu, Peiyuan Huang, Jiayang Gao, Jingxuan Li, Xuejie Wang, Yiren Zhou, Jinhui Wang, Wenting Zhao, Likun Wang, Chaogu Zheng, Bo Gao, Liwen Jiang, Kai Liu, Yusong Guo, Xiaoqiang Yao, Liting Duan
The ability of cells to perceive and respond to mechanical cues is essential for numerous biological activities. Emerging evidence indicates important contributions of organelles to cellular mechanosensitivity and mechanotransduction. However, whether and how the endoplasmic reticulum (ER) senses and reacts to mechanical forces remains elusive. To fill the knowledge gap, after developing a light-inducible ER-specific mechanostimulator (LIMER), we identify that mechanostimulation of ER elicits a transient, rapid efflux of Ca2+ from ER in monkey kidney COS-7 cells, which is dependent on the cation channels transient receptor potential cation channel, subfamily V, member 1 (TRPV1) and polycystin-2 (PKD2) in an additive manner...
April 1, 2024: Developmental Cell