Morgana Barroso Oquendo, Dorothea Siegel-Axel, Felicia Gerst, Estela Lorza-Gil, Anja Moller, Robert Wagner, Jürgen Machann, Falko Fend, Alfred Königsrainer, Martin Heni, Hans-Ulrich Häring, Susanne Ullrich, Andreas L Birkenfeld
Obesity, especially visceral fat accumulation, increases the risk of type 2 diabetes (T2D). The purpose of this study was to investigate the impact of T2D on the pancreatic fat depot. Pancreatic fat pads from 17 partial pancreatectomized patients (PPP) were collected, pancreatic preadipocytes isolated, and in vitro differentiated. Patients were grouped using HbA1c into normal glucose tolerant (NGT), prediabetic (PD), and T2D. Transcriptome profiles of preadipocytes and adipocytes were assessed by RNAseq. Insulin sensitivity was estimated by quantifying AKT phosphorylation on Western blots...
June 1, 2021: American Journal of Physiology. Cell Physiology