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https://www.readbyqxmd.com/read/28821733/three-recombinantly-expressed-apple-tyrosinases-suggest-the-amino-acids-responsible-for-mono-versus-diphenolase-activity-in-plant-polyphenol-oxidases
#1
Ioannis Kampatsikas, Aleksandar Bijelic, Matthias Pretzler, Annette Rompel
Tyrosinases and catechol oxidases belong to the polyphenol oxidase (PPO) enzyme family, which is mainly responsible for the browning of fruits. Three cDNAs encoding PPO pro-enzymes have been cloned from leaves of Malus domestica (apple, MdPPO). The three pro-enzymes MdPPO1-3 were heterologously expressed in E. coli yielding substantial amounts of protein and have been characterized with regard to their optimum of activity resulting from SDS, acidic and proteolytic activation. Significant differences were found in the kinetic characterization of MdPPO1-3 when applying different mono- and diphenolic substrates...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821549/phosphate-catalyzed-hydrogen-peroxide-formation-from-agar-gellan-and-%C3%AE%C2%BA-carrageenan-and-recovery-of-microorganisms-cultivability-by-catalase-and-pyruvate
#2
Kosei Kawasaki, Yoichi Kamagata
Previously, we reported that when agar is autoclaved with phosphate buffer, H2O2 is formed in the resulting medium (PT) and the colony count on the medium inoculated with environmental samples becomes much lower than that on a medium in which agar and phosphate are autoclaved separately (PS) (Appl Environ Microbiol 80:7659-7666). However, the physicochemical mechanisms underlying this observation remain largely unknown. Here, we determined the factors affecting H2O2 formation in agar. The H2O2 formation was pH-dependent: H2O2 was formed at high concentrations in alkaline or neutral phosphate buffer, but not in acidic one...
August 18, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28818123/-women-s-mission-among-women-unacknowledged-origins-of-public-health-nursing
#3
Rima D Apple
No abstract text is available yet for this article.
January 1, 2018: Nursing History Review: Official Journal of the American Association for the History of Nursing
https://www.readbyqxmd.com/read/28818122/evolving-as-necessity-dictates-home-and-public-health-in-the-19th-and-20th-centuries
#4
Rima D Apple, Ciara Breathnach, Linda Bryder, Janet Greenlees
No abstract text is available yet for this article.
January 1, 2018: Nursing History Review: Official Journal of the American Association for the History of Nursing
https://www.readbyqxmd.com/read/28818038/role-of-the-type-vi-secretion-systems-during-disease-interactions-of-erwinia-amylovora-with-its-plant-host
#5
Tim Kamber, Joël F Pothier, Cosima Pelludat, Fabio Rezzonico, Brion Duffy, Theo H M Smits
BACKGROUND: Type VI secretion systems (T6SS) are widespread among Gram-negative bacteria and have a potential role as essential virulence factors or to maintain symbiotic interactions. Three T6SS gene clusters were identified in the genome of E. amylovora CFBP 1430, of which T6SS-1 and T6SS-3 represent complete T6SS machineries, while T6SS-2 is reduced in its gene content. RESULTS: To assess the contribution of T6SSs to virulence and potential transcriptomic changes of E...
August 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28816992/are-physician-assistants-needed-in-guatemala-a-survey-of-potential-urban-and-rural-users
#6
Claudia Luna-Asturias, Jennifer Apple, Guillermo A Bolaños, Jonathan M Bowser, Edwin J Asturias
PURPOSE: The shortage of trained health care personnel has been increasing worldwide. With the physician assistant (PA) profession, created in the United States in the 1960s, expanding globally, this study sought to ascertain whether PAs can be an innovative solution to this crisis. METHODS: We conducted a convenience sample survey to assess the need for and acceptability of future PA professionals in Guatemala. Eighty-nine doctors, nurses, and community members from rural and urban areas of Guatemala participated in the survey...
September 2017: Journal of Physician Assistant Education
https://www.readbyqxmd.com/read/28811498/genome-re-sequencing-reveals-the-history-of-apple-and-supports-a-two-stage-model-for-fruit-enlargement
#7
Naibin Duan, Yang Bai, Honghe Sun, Nan Wang, Yumin Ma, Mingjun Li, Xin Wang, Chen Jiao, Noah Legall, Linyong Mao, Sibao Wan, Kun Wang, Tianming He, Shouqian Feng, Zongying Zhang, Zhiquan Mao, Xiang Shen, Xiaoliu Chen, Yuanmao Jiang, Shujing Wu, Chengmiao Yin, Shunfeng Ge, Long Yang, Shenghui Jiang, Haifeng Xu, Jingxuan Liu, Deyun Wang, Changzhi Qu, Yicheng Wang, Weifang Zuo, Li Xiang, Chang Liu, Daoyuan Zhang, Yuan Gao, Yimin Xu, Kenong Xu, Thomas Chao, Gennaro Fazio, Huairui Shu, Gan-Yuan Zhong, Lailiang Cheng, Zhangjun Fei, Xuesen Chen
Human selection has reshaped crop genomes. Here we report an apple genome variation map generated through genome sequencing of 117 diverse accessions. A comprehensive model of apple speciation and domestication along the Silk Road is proposed based on evidence from diverse genomic analyses. Cultivated apples likely originate from Malus sieversii in Kazakhstan, followed by intensive introgressions from M. sylvestris. M. sieversii in Xinjiang of China turns out to be an "ancient" isolated ecotype not directly contributing to apple domestication...
August 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28807548/application-of-a-simple-column-switching-ion-chromatography-technique-for-removal-of-matrix-interferences-and-sensitive-fluorescence-determination-of-acidic-compounds-pharmaceutical-drugs-in-complex-samples
#8
Nadeem Muhammad, Qamar Subhani, Fenglian Wang, Dandan Guo, Qiming Zhao, Shuchao Wu, Yan Zhu
This work illustrates the introduction of a simple, rugged and flexible column-switching ion chromatography (IC) technique for an automated on-line QuEChERS extracted samples extracts washing followed by sensitive fluorescence (FLD) determination of five acidic pharmaceutical drugs namely; clofibric acid (CLO), ibuprofen (IBU), aspirin (ASP), naproxen (NAP) and flurobrofen (FLU) in three complex samples (spinach, apple and hospital sewage sludge). An old anion exchange column IonPac(®) AS11-HC was utilized as a pre-treatment column for on-line washing of inorganic and organic interferences followed by isocratic separation of five acidic drugs with another anion exchange IonPac(®) AS12A analytical column by exploiting the column-switching technique...
July 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28806430/a-randomized-controlled-trial-of-mastication-with-complete-dentures-made-by-a-conventional-or-an-abbreviated-technique
#9
Cristiane Machado Mengatto, Gustavo Hauber Gameiro, Mario Brondani, C Peter Owen, Michael I MacEntee
PURPOSE: The aim of this randomized clinical trial was to test the hypothesis that there are no statistically significant differences after 3 and 6 months in masticatory performance or chewing ability of people with new complete dentures made by an abbreviated or a conventional technique. MATERIALS AND METHODS: The trial included 20 edentulous participants at a dental school in Brazil assigned randomly to receive dentures made by either a conventional technique involving six clinical sessions or by an abbreviated technique involving three clinical sessions...
August 14, 2017: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/28805446/epigallocatechin-gallate-as-an-anti-obesity-therapeutic-compound-an-in-silico-approach-for-structure-based-drug-designing
#10
Muhammad Shahid Javaid, Noreen Latief, Bushra Ijaz, Usman Ali Ashfaq
Epigallocatechin gallate is a polyphenol of tea plants. Other than tea its trace amounts are found in apple skin, onions and plums. It has anti-adipogenic and anti-oxidant potential. It was investigated that epigallocatechin gallate stopped the adipogenic differentiation of mice mesenchymal stem cells but its underlying mechanism is not well understood. Different proteins and transcription factors responsible for differentiation of adipocytes could be its targets. This study was designed to determine the potential target of epigallocatechin gallate in human...
August 14, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28805053/heterogeneous-photochemistry-of-agrochemicals-at-the-leaf-surface-a-case-study-of-plant-activator-acibenzolar-s-methyl
#11
M Sleiman, P de Sainte Claire, C Richard
The photoreactivity of plant activator benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH), commonly named acibenzolar-S-methyl, was studied on the surfaces of glass, paraffinic wax films, and apple leaves. Experiments were carried out in a solar simulator using pure and formulated BTH (BION). Surface photoproducts were identified using liquid chromatography coupled with electrospray ionization and high-resolution Orbitrap mass spectrometry, while volatile photoproducts were characterized using an online thermal desorption system coupled to a gas chromatography-mass spectrometry (GC-MS) system...
August 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28805042/fabrication-and-characterization-of-electrospun-laminin-functionalized-silk-fibroin-poly-ethylene-oxide-nanofibrous-scaffolds-for-peripheral-nerve-regeneration
#12
Mina Rajabi, Masoumeh Firouzi, Zahra Hassannejad, Ismaeil Haririan, Payam Zahedi
The peripheral nerve regeneration is still one of the major clinical problems, which has received a great deal of attention. In this study, the electrospun silk fibroin (SF)/poly(ethylene oxide) (PEO) nanofibrous scaffolds were fabricated and functionalized their surfaces with laminin (LN) without chemical linkers for potential use in the peripheral nerve tissue engineering. The morphology, surface chemistry, thermal behavior and wettability of the scaffolds were examined to evaluate their performance by means of scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and water contact angle (WCA) measurements, respectively...
August 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28804955/3m-052-as-an-adjuvant-for-a-plga-microparticle-based-leishmania-donovani-recombinant-protein-vaccine
#13
Qian Wang, Meagan A Barry, Christopher A Seid, Elissa M Hudspeth, C Patrick McAtee, Michael J Heffernan
It is believed that an effective vaccine against leishmaniasis will require a T helper type 1 (TH 1) immune response. In this study, we investigated the adjuvanticity of the Toll-like receptor (TLR) 7/8 agonist 3M-052 in combination with the Leishmania donovani 36-kDa nucleoside hydrolase recombinant protein antigen (NH36). NH36 and 3M-052 were encapsulated in separate batches of poly(lactic-co-glycolic acid) (PLGA) microparticles (MPs). The loading efficiency for NH36 was 83% and for 3M-052 was above 95%. In vitro stimulation of bone marrow-derived dendritic cells, measured by IL-12 secretion, demonstrated that 3M-052 (free or MP-formulated) had a concentration-dependent immunostimulatory effect with an optimum concentration of 2 µg/mL...
August 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28801522/vascular-niche-contribution-to-age-associated-neural-stem-cell-dysfunction
#14
Deana Marie Apple, Erzsebet Kokovay
Neural stem cells (NSCs) persist throughout life in the dentate gyrus and the ventricular-subventricular zone (V-SVZ), where they continuously provide new neurons and some glia. These cells are found in specialized niches that regulate quiescence, activation, differentiation, and cell fate choice. A key aspect of the regulatory niche is the vascular plexus, which modulates NSC behavior during tissue homeostasis and regeneration. During aging, NSCs become depleted and dysfunctional, resulting in reduced neurogenesis and poor brain repair...
August 11, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28800823/detection-and-quantification-of-alicyclobacillus-acidoterrestris-by-electrical-impedance-in-apple-juice
#15
Pilar Fernández, José Antonio Gabaldón, Mª Jesús Periago
Alicyclobacillus acidoterrestris is a thermotolerant bacterium able to grow in fruit juices and drinks, as the spoilage by Alicyclobacillus in the final product does not product any gas, but leads to a "medicine flavor" due to the formation of guaicol. Also, its detection is a challenge for the quality control departments, because it takes several days to get the results of traditional microbiology methods. This study aimed at developing a more accurate electrical impedance technique for the detection of A...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800147/reduction-in-bacteremia-after-brushing-with-a-triclosan-copolymer-dentifrice-a-randomized-clinical-study
#16
P K Sreenivasan, D Tischio-Bereski, D H Fine
AIM: The aims of this study were to; 1) test susceptibility to bacteremia in subjects with moderate gingivitis, and 2) compare the effects of brushing with a fluoride toothpaste (control) as compared to a triclosan/copolymer toothpaste (test) on those susceptible to repeated bacteremia. MATERIALS AND METHODS: 107 adult subjects were tested for repeated bacteremia after eating a hard apple. 29 bacteremia positive subjects were enrolled in a double-blind cross-over study designed to analyze the effects of a test toothpaste...
August 11, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28800123/differential-proteomic-analysis-reveals-the-effect-of-calcium-on-malus-baccata-borkh-leaves-under-temperature-stress
#17
Lijie Li, Hong Su, Huaiyu Ma, Deguo Lyu
In the cool apple-producing areas of northern China, air temperature during early spring changes in a rapid and dramatic manner, which affects the growth and development of apple trees at the early stage of the growing season. Previous studies have shown that the treatment of calcium can increase the cold tolerance of Malus baccata Borkh., a widely-used rootstock apple tree in northern China. To better understand the physiological function of calcium in the response of M. baccata to temperature stress, we analyzed the effect of calcium treatment (2% CaCl₂) on M...
August 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28799776/mycotoxin-contamination-of-foods-in-southern-africa-a-10-year-review-2007-2016
#18
J M Misihairabgwi, C N Ezekiel, M Sulyok, G S Shephard, R Krska
Major staple foods in Southern Africa are prone to mycotoxin contamination, posing health risks to consumers and consequent economic losses. Regional climatic zones favor the growth of one or more main mycotoxin producing fungi, Aspergillus, Fusarium and Penicillium. Aflatoxin contamination is mainly reported in maize, peanuts and their products, fumonisin contamination in maize and maize products and patulin in apple juice. Lack of awareness of occurrence and risks of mycotoxins, poor agricultural practices and undiversified diets predispose populations to dietary mycotoxin exposure...
August 11, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28799133/tablets-for-image-review-and-communication-in-daily-routine-of-orthopedic-surgeons-an-evaluation-study
#19
Sven Y Vetter, Svenja Schüler, Matthes Hackbusch, Michael Müller, Benedict Swartman, Marc Schnetzke, Paul Alfred Grützner, Jochen Franke
Medical images are essential in modern traumatology and orthopedic surgery. Access to images is often cumbersome due to a limited number of workstations. Moreover, due to the tremendous increase of data, the time to review or to communicate images has also become limited. One approach to overcome these problems is to make use of modern mobile devices, like tablet computers, to facilitate image access and associated workflows. Ten orthopedic surgeons were equipped with an Apple iPad mini 2 and specialized viewing software for medical images...
August 10, 2017: Journal of Digital Imaging: the Official Journal of the Society for Computer Applications in Radiology
https://www.readbyqxmd.com/read/28798722/autonomic-neuropathy-and-albuminocytologic-dissociation-in-cerebrospinal-fluid-as-the-presenting-features-of-primary-amyloidosis-a-case-report
#20
Jingjing Li, Yi Li, Hongbing Chen, Shihui Xing, Huiyu Feng, Dawei Liu, Dilong Wang, Jinsheng Zeng, Yuhua Fan
OBJECTIVE: Primary amyloidosis is a disease with a poor prognosis and multi-organ involvement. Here, we report the clinical and pathological features of a patient with primary amyloidosis featuring autonomic neuropathy as the initial symptom and albuminocytologic dissociation in the cerebrospinal fluid (CSF). METHODS: The patient was a 60-year-old Chinese male with numbness, orthostatic hypotension, and gastrointestinal symptoms. For diagnosis, we performed an electromyogram (EMG), lumbar puncture, Bence Jones protein urine test, serum electrophoresis blood test, sural nerve and rectal membrane biopsies, transthyretin (TTR) gene sequencing, and bone marrow puncture...
2017: Frontiers in Neurology
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