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https://www.readbyqxmd.com/read/28248237/comparative-skeletal-muscle-proteomics-using-two-dimensional-gel-electrophoresis
#1
REVIEW
Sandra Murphy, Paul Dowling, Kay Ohlendieck
The pioneering work by Patrick H. O'Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry1975, 250, 4007-4021). The application of two-dimensional gel electrophoresis has played a key role in the systematic identification and detailed characterization of the protein constituents of skeletal muscles. Protein changes during myogenesis, muscle maturation, fibre type specification, physiological muscle adaptations and natural muscle aging were studied in depth by the original O'Farrell method or slightly modified gel electrophoretic techniques...
September 9, 2016: Proteomes
https://www.readbyqxmd.com/read/28174246/mechanical-tension-and-spontaneous-muscle-twitching-precede-the-formation-of-cross-striated-muscle-in-vivo
#2
Manuela Weitkunat, Martina Lindauer, Andreas Bausch, Frank Schnorrer
Muscle forces are produced by repetitive stereotyped acto-myosin units called sarcomeres. Sarcomeres are chained into linear myofibrils spanning the entire muscle fiber. In mammalian body muscles, myofibrils are aligned laterally resulting in their typical cross-striated morphology. Despite this detailed textbook knowledge about the adult muscle structure, it is still unclear how cross-striated myofibrils are built in vivo Here, we investigate the morphogenesis of Drosophila abdominal muscles and establish them as in vivo model for cross-striated muscle development...
February 7, 2017: Development
https://www.readbyqxmd.com/read/28130336/peroxisome-proliferator-activated-receptor-%C3%AE-expression-induces-alterations-in-cardiac-myofilaments-in-a-pressure-overload-model-of-hypertrophy
#3
Chehade N Karam, Chad M Warren, Marcus Henze, Natasha Hausler Banke, E Douglas Lewandowski, R John Solaro
Although alterations in fatty acid (FA) metabolism have been shown to have a negative impact on contractility of the hypertrophied heart, the targets of action remain elusive. In this study we compared the function of skinned fiber bundles from transgenic (Tg) mice, that over express a relatively low level of the peroxisome proliferator-activated receptor α (PPARα), and non-transgenic (NTg) littermates. The mice (NTg-T and Tg-T) were stressed by transverse aortic constriction (TAC) and compared to shams (NTg-S and Tg-S)...
January 27, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28126731/prestretched-airway-smooth-muscle-response-to-length-oscillation
#4
Ahmed M Al-Jumaily, Kevin Roos, Sandy Bessaguet, Miguel Jo Avila
Airway smooth muscle (ASM) hyperconstriction is the cause of many respiratory diseases including asthma. In vitro testing has demonstrated that the active forces of ASM are reduced by length oscillation (LO) mimicking tidal breathing. In a previous study, we demonstrated that this force reduction can be further enhanced when superimposing oscillations (with certain frequencies and amplitudes) on this LO In contrast, it has been reported that pressurizing the lung may help in relieving asthmatic airway constrictions...
January 2017: Physiological Reports
https://www.readbyqxmd.com/read/28076581/-social-determinants-of-health-and-convergence-in-health-agendas-of-regional-agencies-in-south-america
#5
Ximena Pamela Díaz-Bermúdez, Flavia Bueno, Luis Francisco Sánchez Otero, Annela Jean Auer
Objective: Characterize areas of interinstitutional cooperation and converging health agendas of the Pan American Health Organization/World Health Organization (PAHO/WHO), the Health Council of the Union of South American Nations (UNASUR), and the Amazon Cooperation Treaty Organization (ACTO) in South America based on social determinants of health. Methods: A qualitative study based on official documentary sources from the three organizations, using a comparative analysis of the health agendas of these agencies and the Rio Political Declaration on Social Determinants of Health (2011)...
November 2016: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/27990827/coordination-between-intra-and-extracellular-forces-regulates-focal-adhesion-dynamics
#6
Bibhu Ranjan Sarangi, Mukund Gupta, Bryant L Doss, Nicolas Tissot, France Lam, René-Marc Mège, Nicolas Borghi, Benoît Ladoux
Focal adhesions (FAs) are important mediators of cell-substrate interactions. One of their key functions is the transmission of forces between the intracellular acto-myosin network and the substrate. However, the relationships between cell traction forces, FA architecture, and molecular forces within FAs are poorly understood. Here, by combining Förster resonance energy transfer (FRET)-based molecular force biosensors with micropillar-based traction force sensors and high-resolution fluorescence microscopy, we simultaneously map molecular tension across vinculin, a key protein in FAs, and traction forces at FAs...
December 23, 2016: Nano Letters
https://www.readbyqxmd.com/read/27942003/biochemical-and-cellular-determinants-of-renal-glomerular-elasticity
#7
Addie E Embry, Hamid Mohammadi, Xinying Niu, Liping Liu, Borren Moe, William A Miller-Little, Christopher Y Lu, Leslie A Bruggeman, Christopher A McCulloch, Paul A Janmey, R Tyler Miller
The elastic properties of renal glomeruli and their capillaries permit them to maintain structural integrity in the presence of variable hemodynamic forces. Measured by micro-indentation, glomeruli have an elastic modulus (E, Young's modulus) of 2.1 kPa, and estimates from glomerular perfusion studies suggest that the E of glomeruli is between 2 and 4 kPa. F-actin depolymerization by latrunculin, inhibition of acto-myosin contractility by blebbistatin, reduction in ATP synthesis, and reduction of the affinity of adhesion proteins by EDTA reduced the glomerular E to 1...
2016: PloS One
https://www.readbyqxmd.com/read/27901489/membrane-bound-icam-1-contributes-to-the-onset-of-proinvasive-tumor-stroma-by-controlling-acto-myosin-contractility-in-carcinoma-associated-fibroblasts
#8
Stephanie Bonan, Jean Albrengues, Eloise Grasset, Sanya-Eduarda Kuzet, Nicolas Nottet, Isabelle Bourget, Thomas Bertero, Bernard Mari, Guerrino Meneguzzi, Cedric Gaggioli
Acto-myosin contractility in carcinoma-associated fibroblasts leads to assembly of the tumor extracellular matrix. The pro-inflammatory cytokine LIF governs fibroblast activation in cancer by regulating the myosin light chain 2 activity. So far, however, how LIF mediates cytoskeleton contractility remains unknown. Using phenotypic screening assays based on knock-down of LIF-dependent genes in fibroblasts, we identified the glycoprotein ICAM-1 as a crucial regulator of stroma fibroblast proinvasive matrix remodeling...
January 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/27861469/-the-national-medical-arbitration-commission-20-years
#9
Juan Ramón de la Fuente
Cuando la Secretaría de Salud todavía tenía el logotipo azul que decía SSA, corrían tiempos difíciles porque no había dinero y la idea de crear nuevas instituciones era recibida con reservas, no sólo por el Presidente Zedillo, siempre generoso y atento a las necesidades de salud de la población, sino también, como ya es costumbre, por el secretario de Hacienda, responsable de cuidar el erario público. Y es que la idea de hacer crecer el gasto corriente del gobierno se percibía, no sin razón, como un acto que podía infligir el riesgo de fomentar estructuras administrativas ineficaces y obesas...
November 2016: Gaceta Médica de México
https://www.readbyqxmd.com/read/27784612/forces-shaping-the-drosophila-wing
#10
REVIEW
M C Diaz de la Loza, B J Thompson
How genes encode the three-dimensional shape of tissues is a fascinating problem in biology. Pioneering genetic studies in the fruit fly Drosophila have identified key genes that control the generation of force patterns in the developing wing. Shortrange force patterns generated by planar polarised myosins can promote boundary formation and tissue elongation during the larval wing disc stage. Long-range force patterns are also crucial to shaping the wing during the pupal stage. We review the different ways in which both local and global force patterns can be generated, such as: patterned acto-myosin contractility, patterned anchorage to the extracellular matrix, and patterned tissue growth...
April 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/27756617/expression-of-profilin-in-trypanosoma-cruzi-and-identification-of-some-of-its-ligands
#11
Juan Felipe Osorio-Méndez, Andrea Vizcaíno-Castillo, Rebeca Manning-Cela, Roberto Hernandez, Ana María Cevallos
The role and regulation of actin in Trypanosoma cruzi and other related parasites is largely unknown. Based on early genome analysis, it was proposed that there was a reduced dependency on the acto-myosin system in the trypanosomatid parasites. However, more recent studies have extended the set of potential actin regulatory proteins, particularly for T. cruzi. One of the identified actin-binding proteins in trypanosomatids is profilin. In other systems, it is capable of simultaneously binding both monomeric actin and several actin-regulatory factors...
October 15, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27671484/two-boundary-first-exit-time-of-gauss-markov-processes-for-stochastic-modeling-of-acto-myosin-dynamics
#12
Giuseppe D'Onofrio, Enrica Pirozzi
We consider a stochastic differential equation in a strip, with coefficients suitably chosen to describe the acto-myosin interaction subject to time-varying forces. By simulating trajectories of the stochastic dynamics via an Euler discretization-based algorithm, we fit experimental data and determine the values of involved parameters. The steps of the myosin are represented by the exit events from the strip. Motivated by these results, we propose a specific stochastic model based on the corresponding time-inhomogeneous Gauss-Markov and diffusion process evolving between two absorbing boundaries...
September 26, 2016: Journal of Mathematical Biology
https://www.readbyqxmd.com/read/27642290/machine-learning-to-predict-rapid-progression-of-carotid-atherosclerosis-in-patients-with-impaired-glucose-tolerance
#13
Xia Hu, Peter D Reaven, Aramesh Saremi, Ninghao Liu, Mohammad Ali Abbasi, Huan Liu, Raymond Q Migrino
OBJECTIVES: Prediabetes is a major epidemic and is associated with adverse cardio-cerebrovascular outcomes. Early identification of patients who will develop rapid progression of atherosclerosis could be beneficial for improved risk stratification. In this paper, we investigate important factors impacting the prediction, using several machine learning methods, of rapid progression of carotid intima-media thickness in impaired glucose tolerance (IGT) participants. METHODS: In the Actos Now for Prevention of Diabetes (ACT NOW) study, 382 participants with IGT underwent carotid intima-media thickness (CIMT) ultrasound evaluation at baseline and at 15-18 months, and were divided into rapid progressors (RP, n = 39, 58 ± 17...
December 2016: EURASIP Journal on Bioinformatics & Systems Biology
https://www.readbyqxmd.com/read/27635194/toxicological-evaluation-of-subchronic-use-of-pioglitazone-in-mice
#14
Said Said Elshama, Ayman El-Meghawry El-Kenawy, Hosam-Eldin Hussein Osman
OBJECTIVES: Pioglitazone (Actos) is one of the most controversial recent oral antidiabetic drugs. It was originally authorized in the European Union in 2000, and approved as an oral monotherapy for overweight second type of diabetic patients in 2002. It belongs to the thiazolidinedione group which some of its members have been withdrawn from the market due to the hepatotoxicity or cardiotoxicity effects. This study investigates sub-chronic use of pioglitazone induced toxicity in mice by the assessment of renal and liver function tests, cardiac enzymes, and some hematological indices with histological changes of liver, kidney, heart, and bladder...
July 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27598375/sexual-violence-as-a-limiting-factor-on-the-perception-and-management-of-the-risk-of-hiv-in-women-married-to-migrants
#15
Yesica Yolanda Rangel Flores
OBJECTIVE: to analyze the influence of sexual violence on the perception and management of the risk of HIV in women married to migrants. METHODS: study with an ethnographic approach carried out in urban and rural communities. Data were obtained by methodological triangulation, with participant and non-participant observation, as well as interviews. The informants were 21 women married to international migrants. The interviews were transcribed and discourse analysis was applied to them...
September 1, 2016: Revista Latino-americana de Enfermagem
https://www.readbyqxmd.com/read/27541056/apkc%C3%AE-affects-directed-cell-migration-through-the-regulation-of-myosin-light-chain-phosphorylation
#16
Daria Petrov, Inbal Dahan, Einav Cohen-Kfir, Shoshana Ravid
Cell motility is an essential cellular process for a variety of biological events. It requires cross-talk between the signaling and the cytoskeletal systems. Despite the recognized importance of aPKCζ for cell motility, there is little understanding of the mechanism by which aPKCζ mediates extracellular signals to the cytoskeleton. In the present study, we report that aPKCζ is required for the cellular organization of acto-non-muscle myosin II (NMII) cytoskeleton, for proper cell adhesion and directed cell migration...
August 19, 2016: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/27498166/traction-force-dynamics-predict-gap-formation-in-activated-endothelium
#17
Erik T Valent, Geerten P van Nieuw Amerongen, Victor W M van Hinsbergh, Peter L Hordijk
In many pathological conditions the endothelium becomes activated and dysfunctional, resulting in hyperpermeability and plasma leakage. No specific therapies are available yet to control endothelial barrier function, which is regulated by inter-endothelial junctions and the generation of acto-myosin-based contractile forces in the context of cell-cell and cell-matrix interactions. However, the spatiotemporal distribution and stimulus-induced reorganization of these integral forces remain largely unknown. Traction force microscopy of human endothelial monolayers was used to visualize contractile forces in resting cells and during thrombin-induced hyperpermeability...
September 10, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27447488/timely-endocytosis-of-cytokinetic-enzymes-prevents-premature-spindle-breakage-during-mitotic-exit
#18
Cheen Fei Chin, Kaiquan Tan, Masayuki Onishi, YuanYuan Chew, Beryl Augustine, Wei Ren Lee, Foong May Yeong
Cytokinesis requires the spatio-temporal coordination of membrane deposition and primary septum (PS) formation at the division site to drive acto-myosin ring (AMR) constriction. It has been demonstrated that AMR constriction invariably occurs only after the mitotic spindle disassembly. It has also been established that Chitin Synthase II (Chs2p) neck localization precedes mitotic spindle disassembly during mitotic exit. As AMR constriction depends upon PS formation, the question arises as to how chitin deposition is regulated so as to prevent premature AMR constriction and mitotic spindle breakage...
July 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27421270/new-pharmacological-agents-to-aid-smoking-cessation-and-tobacco-harm-reduction-what-has-been-investigated-and-what-is-in-the-pipeline
#19
REVIEW
Emma Beard, Lion Shahab, Damian M Cummings, Susan Michie, Robert West
A wide range of support is available to help smokers to quit and to aid attempts at harm reduction, including three first-line smoking cessation medications: nicotine replacement therapy, varenicline and bupropion. Despite the efficacy of these, there is a continual need to diversify the range of medications so that the needs of tobacco users are met. This paper compares the first-line smoking cessation medications with (1) two variants of these existing products: new galenic formulations of varenicline and novel nicotine delivery devices; and (2) 24 alternative products: cytisine (novel outside Central and Eastern Europe), nortriptyline, other tricyclic antidepressants, electronic cigarettes, clonidine (an anxiolytic), other anxiolytics (e...
October 2016: CNS Drugs
https://www.readbyqxmd.com/read/27383012/game-of-zones-how-actin-binding-proteins-organize-muscle-contraction
#20
COMMENT
Eugenia Butkevich, Dieter R Klopfenstein, Christoph F Schmidt
Locomotion of C. elegans requires coordinated, efficient transmission of forces generated on the molecular scale by myosin and actin filaments in myocytes to dense bodies and the hypodermis and cuticle enveloping body wall muscles. The complex organization of the acto-myosin scaffold with its accessory proteins provides a fine-tuned machinery regulated by effectors that guarantees that sarcomere units undergo controlled, reversible cycles of contraction and relaxation. Actin filaments in sarcomeres dynamically undergo polymerization and depolymerization...
April 2016: Worm
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