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https://www.readbyqxmd.com/read/29458079/autofluorescence-a-potential-pitfall-in-immunofluorescence-based-inflammation-grading
#1
Jonas Wizenty, Muhammad Imtiaz Ashraf, Nadine Rohwer, Martin Stockmann, Sascha Weiss, Matthias Biebl, Johann Pratschke, Felix Aigner, Tilo Wuensch
Immunofluorescence (IF) staining of paraffin-embedded tissues is a frequently used method to answer research questions or even detect the abundance of a certain protein for diagnostic use. However, the signal originating from specific antibody-staining might be distorted by autofluorescence (AF) of the assessed tissue. Although the AF phenomenon is well known, its presence is often neglected by insufficient staining controls. In this study, we describe the existence of cellular AF in paraffin-embedded healthy and inflamed human and murine colonic tissues and present ways to reduce AF...
February 16, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29457520/caregiver-reactions-to-aggressive-behaviors-in-persons-with-dementia-in-a-diverse-community-dwelling-sample
#2
Bryan R Hansen, Nancy A Hodgson, Chakra Budhathoki, Laura N Gitlin
PURPOSE: To describe caregiver challenges with and confidence managing three aggressive behavior types in persons with dementia: verbal aggression, destroying property, and threatening to hurt others. DESIGN AND METHOD: Secondary analysis of baseline data from the 2001-2004 Resources for Enhancing Alzheimer's Caregiver Health II (REACH II) initiative. RESULTS: One or more aggressive behaviors within 1 week were reported by more than a third of caregivers, with most expressing upset but fewer expressing confidence managing the behaviors...
February 1, 2018: Journal of Applied Gerontology: the Official Journal of the Southern Gerontological Society
https://www.readbyqxmd.com/read/29457468/experiences-of-racism-sexual-objectification-and-alcohol-use-among-gay-and-bisexual-men-of-colour
#3
Rusty Souleymanov, David J Brennan, Clemon George, Richard Utama, Andre Ceranto
OBJECTIVE: Previous research has shown that experiences of racial discrimination and sexual objectification are associated with health risk behaviours among gay and bisexual men of colour. However, little is known about whether racial discrimination and sexual objectification are associated with alcohol use among this population. This community-based study examined the association between racial discrimination, sexual objectification and alcohol use in a sample of 369 gay and bisexual men of colour (Black/African/Caribbean, Latino/Latin American, South Asian, and East and Southeast Asian) in Toronto...
February 18, 2018: Ethnicity & Health
https://www.readbyqxmd.com/read/29457370/avian-metapneumovirus-subtype-c-in-wild-waterfowl-in-ontario-canada
#4
C M Jardine, E J Parmley, T Buchanan, L Nituch, D Ojkic
Avian metapneumovirus (aMPV) is an emerging poultry pathogen that has a significant economic impact on poultry production worldwide. The geographic range of the virus continues to expand, and wild birds have been implicated as reservoirs of aMPV that have the potential to spread the virus over long distances. Our objective was to determine the apparent prevalence of aMPV subtype C in wild waterfowl in Ontario, Canada. Wild waterfowl were captured in August and September, 2016 as part of routine migratory waterfowl population monitoring by the Ontario Ministry of Natural Resources and Forestry...
February 18, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29457272/atopic-dermatitis-in-diverse-racial-and-ethnic-groups-variations-in-epidemiology-genetics-clinical-presentation-and-treatment
#5
REVIEW
Bridget P Kaufman, Emma Guttman-Yassky, Andrew F Alexis
Atopic dermatitis (AD) is a chronic inflammatory skin condition that affects diverse ethnic groups with varying prevalence. Despite a predominance of studies in individuals of European ancestry, AD has been found to occur more frequently in Asian and black individuals than whites. Therefore, an understanding of the unique clinical features of AD in diverse ethnic groups, as well as the differences in genetic polymorphisms that influence susceptibility to AD and response to current therapies, is paramount for management of an increasingly diverse patient population...
February 19, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29457011/trichoscopic-features-of-linear-morphea-on-the-scalp
#6
David Saceda-Corralo, Antonella Tosti
Linear scleroderma en coup de sabre (LSCS) is the most common form of morphea on the scalp and its trichoscopic features have not been described yet. We report 2 adult women with LSCS with distinct dermoscopic findings. They both had an atrophic well-defined linear patch of alopecia on the frontotemporal scalp. Dermoscopy showed loss of follicular openings on a whitish skin surface; scattered black dots, broken hairs, and pili torti; and short thick linear and branching tortuous vessels on the periphery of the lesion...
January 2018: Skin Appendage Disorders
https://www.readbyqxmd.com/read/29456975/self-rated-health-and-mortality-due-to-kidney-diseases-racial-differences-in-the-united-states
#7
Shervin Assari
Background: Although the role of self-rated health (SRH) on all-cause mortality is known, we still do not know whether SRH predicts death due to specific causes (e.g., kidney disease). The current study aimed to compare Blacks and Whites on the association between SRH and mortality due to kidney diseases. A nationally representative sample of adults in the United States was used to provide generalizable results to the United States population. Materials and Methods: The Americans' Changing Lives study is a nationally representative cohort, conducted from 1986-2011...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29456878/non-destructive-two-photon-excited-fluorescence-imaging-identifies-early-nodules-in-calcific-aortic-valve-disease
#8
Lauren M Baugh, Zhiyi Liu, Kyle P Quinn, Sam Osseiran, Conor L Evans, Gordon S Huggins, Philip W Hinds, Lauren D Black, Irene Georgakoudi
Calcifications occur during the development of healthy bone, and at the onset of calcific aortic-valve disease (CAVD) and many other pathologies. Although the mechanisms regulating early calcium deposition are not fully understood, they may provide targets for new treatments and for early interventions. Here, we show that two-photon excited fluorescence (TPEF) can provide quantitative and sensitive readouts of calcific nodule formation, in particular in the context of CAVD. Specifically, by means of the decomposition of TPEF spectral images from excised human CAVD valves and from rat bone prior to and following demineralization, as well as from calcific nodules formed within engineered gels, we identified an endogenous fluorophore that correlates with the level of mineralization in the samples...
November 2017: Nature Biomedical Engineering
https://www.readbyqxmd.com/read/29456651/protective-effect-of-black-garlic-extracts-on-tert-butyl-hydroperoxide-induced-injury-in-hepatocytes-via-a-c-jun-n-terminal-kinase-dependent-mechanism
#9
Ko-Chao Lee, Chih-Chuan Teng, Chien-Heng Shen, Wen-Shih Huang, Chien-Chang Lu, Hsing-Chun Kuo, Shui-Yi Tung
Black garlic has been reported to show multiple bioactivities against the development of different diseases. In the present study, the hepatoprotective effect of black garlic on injured liver cells was investigated. Rat clone-9 hepatocytes were used for all experiments; tert-Butyl hydroperoxide (tBHP) was used to induce injury of rat clone-9 hepatocytes. The contents of malondialdehyde (MDA) and glutathione (GSH); anti-oxidative enzyme activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx); and mRNA expression levels of interleukin (IL)-6 and IL-8 in rat clone-9 hepatocytes were determined to evaluate the level of cell damage...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29456384/tea-polyphenols-and-prevention-of-epigenetic-aberrations-in-cancer
#10
REVIEW
Arundhati Bag, Niladri Bag
Tea polyphenols are secondary metabolites of tea plants and are well known for beneficial health effects. They can protect from a variety of illnesses including cancers. Tea polyphenols can prevent cancer by modulating epigenetic aberrations taking place in DNA methylation, histone modifications, and micro-RNAs. By altering these epimutations, they regulate chromatin dynamics and expression of genes those induce or suppress cancer formation. However, majority of the studies in existing literature are carried out for green tea polyphenols rather than black tea polyphenols despite the fact that black tea is the most commonly consumed form of tea (78%) followed by green tea (20%) and other forms of tea...
January 2018: Journal of Natural Science, Biology, and Medicine
https://www.readbyqxmd.com/read/29455738/no-experience-required-violent-crime-and-anticipated-vicarious-and-experienced-racial-discrimination
#11
Daniel Herda, Bill McCarthy
There is a growing body of evidence linking racial discrimination and juvenile crime, and a number of theories explain this relationship. In this study, we draw on one popular approach, Agnew's general strain theory, and extend prior research by moving from a focus on experienced discrimination to consider two other forms, anticipated and vicarious discrimination. Using data on black, white, and Hispanic youth, from the Project on Human Development in Chicago Neighborhoods (PHDCN), we find that experienced, anticipated, and to a lesser extent, vicarious discrimination, significantly predict violent crime independent of a set of neighborhood, parental, and individual level controls, including prior violent offending...
February 2018: Social Science Research
https://www.readbyqxmd.com/read/29455737/foreclosure-migration-and-neighborhood-outcomes-moving-toward-segregation-and-disadvantage
#12
Matthew Hall, Kyle Crowder, Amy Spring, Ryan Gabriel
The US housing crisis during the late 2000s was arguably the most devastating residential disaster of the last century, sending millions of families into foreclosure and destroying billions in household wealth. An understudied aspect of the crisis was the spike in local migration that followed the foreclosure surge. In this paper, we assess the residential consequences of these moves, by exploring how foreclosure-induced migration affected the racial and socioeconomic composition of affected families' neighborhoods...
February 2018: Social Science Research
https://www.readbyqxmd.com/read/29455727/enhancement-of-gut-permeation-of-amoxicillin-with-nigella-sativa-seed-extract-and-its-phytochemical-screening
#13
Babar Ali, Mohammad Ali, Saima Amin, Showkat R Mir
The seeds of Nigella sativa Linn. (Ranunculaceae), commonly known as Black cumin, are predominantly used as carminative, antispasmodic, and stimulant. The main objective of the present study was to evaluate the effect of N. sativa seed extract on the permeation of co-infused amoxicillin across the gut wall. The methanolic extract of N. sativa improved intestinal permeability of amoxicillin in in-vitro experiments in a dose-dependent manner. Two new glycosides, decanyl nigelloic acid diglucoside [n-decanyl-3-aldehydic-4-methoxy-5-hydroxy benzoate-5-β-D-glucofuranosyl (2→1)-β-D-glucopyranosyl-(2→1)-β-D-glucopyranoside]] and nigelabdienoyl triglucoside [homo-labd-5, 9(11)-dien-16-onyl-β-D-glucopyranosyl (2→1)-β-D-glucopyranosyl (2→1)-β-D-glucopyranoside], along with seven known fatty acid glycerides/esters, were isolated from the gut permeation enhancing extract...
February 2018: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29455553/correlates-of-missed-clinic-visits-among-youth-living-with-hiv
#14
Nicholas Tarantino, Larry K Brown, Laura Whiteley, M Isabel Fernández, Sharon L Nichols, Gary Harper
Youth living with HIV (YLH) have significant problems with attending their medical appointments. Poor attendance, consequently, predicts viral non-suppression and other negative health outcomes. To identity targets of intervention, this cross-sectional study examined correlates of past-year missed clinic visits among YLH (N = 2125) attending HIV clinics in the United States and Puerto Rico. Thirty-six percent of YLH missed two or more visits in the past year. Several factors were associated with missed visits in our regression model...
February 17, 2018: AIDS Care
https://www.readbyqxmd.com/read/29455524/multiwall-carbon-nanotubes-induce-more-pronounced-transcriptomic-responses-in-pseudomonas-aeruginosa-pg201-than-graphene-exfoliated-boron-nitride-or-carbon-black
#15
Monika Mortimer, Naresh Devarajan, Dong Li, Patricia A Holden
Carbonaceous and boron nitride (BN) nanomaterials have similar applications and hydrophobic properties suggesting common release pathways and exposure to bacteria. While high nanomaterial concentrations can be bactericidal or growth-inhibitory, little is known regarding bacterial transcriptional responses to non-growth-inhibitory nanomaterial concentrations. Here, using one strain of Pseudomonas aeruginosa-a clinically and environmentally important bacterial taxon-we analyzed the comparative transcriptomic response to carbonaceous or BN nanomaterials...
February 19, 2018: ACS Nano
https://www.readbyqxmd.com/read/29455467/predicting-reintroduction-outcomes-for-highly-vulnerable-species-that-do-not-currently-coexist-with-their-key-threats
#16
Elizabeth H Parlato, Doug P Armstrong
Predicting reintroduction outcomes before populations are released is inherently challenging. It becomes even more difficult when the species being considered for reintroduction no longer co-exists with the key threats limiting its distribution. However, data from other species facing the same threats can be used to make predictions under these circumstances. We present an integrated Bayesian modelling approach for predicting growth of a reintroduced population at a range of predator densities when no data are available for the species in the presence of that predator...
February 17, 2018: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/29455465/insulin-like-growth-factor-2-a-poor-prognostic-biomarker-linked-to-racial-disparity-in-women-with-uterine-carcinosarcoma
#17
Anne R Van Arsdale, Rebecca C Arend, Maria J Cossio, Britt K Erickson, Yanhua Wang, David W Doo, Charles A Leath, Gary L Goldberg, Gloria S Huang
The objective of this study was to investigate the relationship of insulin-like growth factor 2 (IGF2) expression and survival in women with uterine carcinosarcoma (UCS). Insulin-like growth factor 2 protein expression was determined by immunohistochemical staining of tumor tissues from 103 patients with UCS. The H-score (product of staining intensity and percentage positive cells) was quantified for the epithelial cytoplasmic (EC), epithelial nuclear (EN), and malignant stromal compartments. Multivariable Cox proportional hazard regression models were used to examine the relationship of IGF2 levels with progression-free survival (PFS) and overall survival (OS)...
February 18, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29455412/geert-jan-rutten-the-broca-wernicke-doctrine-a-historical-and-clinical-perspective-on-localization-of-language-functions-springer-international-publishing-2017-e-book-isbn-978-3-319-54633-9-ebook-%C3%A2-83-hardcover-%C3%A2-104-78-black-white-illustrations-38-color-illustrations
#18
https://www.readbyqxmd.com/read/29455357/phytochemical-constituents-antioxidant-activity-and-antiproliferative-properties-of-black-red-and-brown-rice-bran
#19
Ali Ghasemzadeh, Mohamad Taghi Karbalaii, Hawa Z E Jaafar, Asmah Rahmat
BACKGROUND: In the recent years, the health benefits of the pigmented rice varieties have reported due to the presence of bioactive compounds. In this study, the phytochemical constituents (total phenolic, flavonoid and anthocyanin content) and individual phenolics and flavonoids of the extracts of sixteen genotypes of pigmented rice bran were evaluated using spectrophotometric and ultra-high performance liquid chromatography method. Antioxidative properties of the free and bound fractions were evaluated using nitric oxide and 1,1-diphenyl-2-picrylhydrazyl scavenging assays...
February 17, 2018: Chemistry Central Journal
https://www.readbyqxmd.com/read/29455248/are-there-effects-of-age-gender-height-and-body-fat-on-the-functional-muscle-bone-unit-in-children-and-adults
#20
I Duran, K Martakis, S Hamacher, C Stark, O Semler, E Schoenau
The aim was to describe the effect of age, gender, height, different stages of human life, and body fat on the functional muscle-bone unit. All these factors had a significant effect on the functional muscle-bone unit and should be addressed when assessing functional muscle-bone unit in children and adults. INTRODUCTION: For the clinical evaluation of the functional muscle-bone unit, it was proposed to evaluate the adaptation of the bone to the acting forces. A frequently used parameter for this is the total body less head bone mineral content (TBLH-BMC) determined by dual-energy X-ray absorptiometry (DXA) in relation to the lean body mass (LBM by DXA)...
February 17, 2018: Osteoporosis International
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