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https://www.readbyqxmd.com/read/28719740/tuning-superhydrophobic-nanostructures-to-enhance-jumping-droplet-condensation
#1
Megan D Mulroe, Bernadeta R Srijanto, S Farzad Ahmadi, C Patrick Collier, Jonathan B Boreyko
It was recently discovered that condensation growing on a nanostructured superhydrophobic surface can spontaneously jump off the surface, triggered by naturally occurring coalescence events. Many reports have observed that droplets must grow to a size of order 10 μm before jumping is enabled upon coalescence; however, it remains unknown how the critical jumping size relates to the topography of the underlying nanostructure. Here, we characterize the dynamic behavior of condensation growing on six different superhydrophobic nanostructures, where the topography of the nanopillars was systematically varied...
July 18, 2017: ACS Nano
https://www.readbyqxmd.com/read/28718081/blastocyst-utilization-rates-after-continuous-culture-in-two-commercial-single-step-media-a-prospective-randomized-study-with-sibling-oocytes
#2
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, Christos A Venetis, George K Petsas, Basil C Tarlatzis, Tryfon G Lainas
PURPOSE: The aim of this study is to determine whether blastocyst utilization rates are different after continuous culture in two different commercial single-step media. METHODS: This is a paired randomized controlled trial with sibling oocytes conducted in infertility patients, aged ≤40 years with ≥10 oocytes retrieved assigned to blastocyst culture and transfer. Retrieved oocytes were randomly allocated to continuous culture in either Sage one-step medium (Origio) or Continuous Single Culture (CSC) medium (Irvine Scientific) without medium renewal up to day 5 post oocyte retrieval...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28718080/a-comparison-of-live-birth-rates-and-perinatal-outcomes-between-cryopreserved-oocytes-and-cryopreserved-embryos
#3
Jacqueline R Ho, Irene Woo, Kristin Louie, Wael Salem, Sami I Jabara, Kristin A Bendikson, Richard J Paulson, Karine Chung
PURPOSE: Prior studies suggest that pregnancy outcomes after autologous oocyte cryopreservation are similar to fresh in vitro fertilization (IVF) cycles. It is unknown whether there are differences in pregnancy and perinatal outcomes between cryopreserved oocytes and cryopreserved embryos. METHODS: This is a retrospective cohort study comparing pregnancy and perinatal outcomes between oocyte and embryo cryopreservation at a university-based fertility center. We included 42 patients and 68 embryo transfers in patients who underwent embryo transfer after elective oocyte preservation (frozen oocyte-derived embryo transfer (FOET)) from 2005 to 2015...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28715087/association-between-endometrial-subendometrial-vasculature-and-embryo-transfer-outcome-a-meta-analysis-and-subgroup-analysis
#4
Jianing Wang, Fei Xia, Ying Zhou, Xuedong Wei, Yanyan Zhuang, Yingxue Huang
OBJECTIVES: To examine the association between endometrial/subendometrial vasculature and in vitro fertilization-embryo transfer (IVF-ET) and frozen embryo transfer (FET) outcomes. METHODS: A meta-analysis of studies using endometrial/subendometrial 3-dimensional ultrasound and power Doppler angiography was performed to examine the vascularization index (VI), flow index (FI), and vascularization-flow index (VFI) in pregnant and nonpregnant women. Ten articles were analyzed, including 895 pregnant women and 882 nonpregnant women...
July 17, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28714052/conservation-of-phenotypes-in-the-roman-high-and-low-avoidance-rat-strains-after-embryo-transfer
#5
Cristóbal Río-Álamos, Cristina Gerbolés, Carles Tapias-Espinosa, Daniel Sampedro-Viana, Ignasi Oliveras, Ana Sánchez-González, Toni Cañete, Gloria Blázquez, María Del Mar López, Carlos Baldellou, Pedro J Otaegui, Adolf Tobeña, Alberto Fernández-Teruel
The Roman high- (RHA-I) and low-avoidance (RLA-I) rat strains are bi-directionally bred for their good versus non-acquisition of two-way active avoidance, respectively. They have recently been re-derived through embryo transfer (ET) to Sprague-Dawley females to generate specific pathogen free (SPF) RHA-I/RLA-I rats. Offspring were phenotyped at generations 1 (G1, born from Sprague-Dawley females), 3 and 5 (G3 and G5, born from RHA-I and RLA-I from G2-G4, respectively), and compared with generation 60 from our non-SPF colony...
July 17, 2017: Behavior Genetics
https://www.readbyqxmd.com/read/28712374/parthenogenetic-activation-and-somatic-cell-nuclear-transfer-of-porcine-oocytes-activated-by-an-electric-pulse-and-azd5438-treatment
#6
Xiao-Chen Li, Qing Guo, Hai-Ying Zhu, Long Jin, Yu-Chen Zhang, Guang-Lei Zhang, Xiao-Xu Xing, Mei-Fu Xuan, Qi-Rong Luo, Zhao-Bo Luo, Jun-Xia Wang, Cheng-Du Cui, Wen-Xue Li, Zheng-Yun Cui, Xi-Jun Yin, Jin-Dan Kang
We examined the in vitro developmental competence of parthenogenetic activation (PA) oocytes activated by an electric pulse (EP) and treated with various concentrations of AZD5438 for 4 h. Treatment with 10 µM AZD5438 for 4 h significantly improved the blastocyst formation rate of PA oocytes in comparison with 0, 20, or 50 µM AZD5438 treatment (46.4% vs. 34.5%, 32.3%, and 24.0%, respectively; P 0.05). Furthermore, 66.67% of blastocysts derived from these AZD5438-treated PA oocytes had a diploid karyotype...
July 17, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28712062/genome-editing-and-genetic-engineering-in-livestock-for-advancing-agricultural-and-biomedical-applications
#7
Bhanu P Telugu, Ki-Eun Park, Chi-Hun Park
Genetic modification of livestock has a longstanding and successful history, starting with domestication several thousand years ago. Modern animal breeding strategies predominantly based on marker-assisted and genomic selection, artificial insemination, and embryo transfer have led to significant improvement in the performance of domestic animals, and are the basis for regular supply of high quality animal derived food. However, the current strategy of breeding animals over multiple generations to introduce novel traits is not realistic in responding to the unprecedented challenges such as changing climate, pandemic diseases, and feeding an anticipated 3 billion increase in global population in the next three decades...
July 15, 2017: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/28711250/genetic-analysis-for-quality-of-frozen-embryos-produced-by-holstein-cattle-donors-in-canada
#8
C Jaton, F S Schenkel, F Malchiodi, M Sargolzaei, C A Price, C Baes, F Miglior
The number of embryos produced by Holstein donors has been shown to be heritable, so it could be possible to genetically select for this trait to improve the efficiency of the assisted reproductive technology (ART) in dairy cattle. Another important parameter to consider for achieving good results from ART is embryo quality because embryos of good quality have more chance of producing live offspring. The possibility of using genetic selection for increasing the quality of embryo produced from ART has yet to be assessed...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28708536/relationship-among-circulating-anti-m%C3%A3-llerian-hormone-insulin-like-growth-factor-1-cadmium-and-superovulatory-response-in-dairy-cows
#9
R L Abdel Aziz, A A Y Khalil, A Abdel-Wahab, N Y Hassan, E Abdel-Hamied, R K Kasimanickam
The objectives of this study were 1. to determine the associations among circulating anti-Mullerian hormone (AMH), insulin like growth factor 1 (IGF1) and cadmium (Cd) concentrations of lactating Holstein cows at the time of superovulation and 2. to determine the effect of circulating AMH, IGF1 and Cd concentrations on the superovulatory response in Holstein dairy cows. Holstein cows (n = 30) were milked thrice daily and housed and fed in free stall barn as a separate group. All animals were synchronized for superovulation and flushed...
June 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28706605/embryonic-stem-cell-conditioned-medium-supports-in-vitro-maturation-of-mouse-oocytes
#10
Saber Miraki, Aram Mokarizadeh, Omid Banafshi, Vahideh Assadollahi, Mahdad Abdi, Daem Roshani, Fardin Fathi
BACKGROUND: This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). METHODS: Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized in vitro...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28703935/the-effect-of-laparoscopic-resection-of-large-niches-in-the-uterine-caesarean-scar-on-symptoms-ultrasound-findings-and-quality-of-life-a-prospective-cohort-study
#11
A J M W Vervoort, J Vissers, W J K Hehenkamp, H A M Brölmann, J A F Huirne
OBJECTIVE: To evaluate the effectiveness of a laparoscopic niche resection on niche related symptoms and/or fertility related problems, ultrasound findings and quality of life. DESIGN: Prospective cohort study. SETTING: University hospital. POPULATION: Women with a large niche (residual myometrium (RM) <3 mm) and complaints of either postmenstrual spotting, dysmenorrhea, intrauterine fluid accumulation and/or difficulties with embryo transfer due to distorted anatomy...
July 13, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28702979/use-of-platelet-rich-plasma-in-human-infertility
#12
G V L Colombo, V Fanton, D Sosa, E Criado Scholz, J Lotti, S E Aragona, T Lotti
Successful embryo implantation requires good quality embryo but also needs a receptive endometrium site. In our clinical practice, we daily verify that an adequate endometrial growth is reached for successful implantation. To understand whether platelet rich plasma (PRP) can improve endometrium thickness and performance, PRP treatment was carried out after at least three of the classic medical protocols currently in use had been unsuccessfully adopted. Eight patients with more than 3 cryo-transfers cancelled because of failure of endometrial growth, defined as endometrium less than 6 mm, with negative hysteroscopic screening for endometrial pathology, and with negative bacteriologic screening, before present and all previous treatment, were selected to undergo PRP treatment...
July 13, 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28701237/flow-cytometric-sex-sorting-affects-cd4-membrane-distribution-and-binding-of-exogenous-dna-on-bovine-sperm-cells
#13
William Borges Domingues, Tony Leandro Rezende da Silveira, Eliza Rossi Komninou, Leonardo Garcia Monte, Mariana Härter Remião, Odir Antônio Dellagostin, Carine Dahl Corcini, Antônio Sergio Varela Junior, Fabiana Kömmling Seixas, Tiago Collares, Vinicius Farias Campos
Bovine sex-sorted sperm have been commercialized and successfully used for the production of transgenic embryos of the desired sex through the sperm-mediated gene transfer (SMGT) technique. However, sex-sorted sperm show a reduced ability to internalize exogenous DNA. The interaction between sperm cells and the exogenous DNA has been reported in other species to be a CD4-like molecule-dependent process. The flow cytometry-based sex-sorting process subjects the spermatozoa to different stresses causing changes in the cell membrane...
July 13, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28697910/duphaston-and-human-menopausal-gonadotropin-protocol-in-normally-ovulatory-women-undergoing-controlled-ovarian-hyperstimulation-during-in%C3%A2-vitro-fertilization-intracytoplasmic-sperm-injection-treatments-in-combination-with-embryo-cryopreservation
#14
Xiuxian Zhu, Hongjuan Ye, Yonglun Fu
OBJECTIVE: To evaluate endocrine characteristics and clinical outcomes in normal ovulatory patients undergoing controlled ovarian hyperstimulation (COH) with the use of a Duphaston and hMG protocol during in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatments in combination with frozen-thawed embryo transfer (FET) compared with the characteristics and outcomes of patients undergoing an Utrogestan and hMG protocol. DESIGN: Prospective controlled study...
July 8, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28695395/a-meta-analysis-of-atosiban-supplementation-among-patients-undergoing-assisted-reproduction
#15
Jie Li, Yang Chen, Anran Wang, Haiying Zhang
OBJECTIVES: Evidence for the efficacy of atosiban in improving pregnancy outcomes among patients undergoing assisted reproductive technology (ART) remains inconsistent. METHODS: The PubMed, EMBASE, and CNKI databases were searched using keywords such as 'atosiban', 'infertility', and 'ART'. Studies that explored the efficacy of atosiban in the field of ART for patients with embryo transfer (ET) were included if they evaluated pregnancy outcomes. Combing using subgroups and sensitivity analysis were conducted, and risk ratios (RRs), and 95% confidence intervals (CIs) were calculated...
July 10, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28693296/mineralized-deposits-in-the-uterus-of-a-pig-without-pregnancy-loss
#16
Geon A Kim, Jun-Xue Jin, Anukul Taweechaipaisankul, Sanghoon Lee, Byung Il Yoon, Jongki Cho, Byeong Chun Lee
We reported a case of uterine calcification in the uterus of a pig without pregnancy loss. The recipient underwent cloned embryo transfer and the Cesarean section for safe delivery of cloned piglets. During the Cesarean section, 4 white, starlike, 2 x 2 x 2 cm, calcified structures were found within the endometrial cavity. Despite dystrophic calcification around placenta, healthy cloned piglets were produced successfully. To our knowledge, this is the first reported case of dystrophic calcification occurring within the uterus in a pregnant pig...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28689997/dynamic-reorganization-of-nucleosome-positioning-in-somatic-cells-after-transfer-into-porcine-enucleated-oocytes
#17
Chenyu Tao, Juan Li, Xia Zhang, Baobao Chen, Daming Chi, Yaqiong Zeng, Yingjie Niu, Chengfei Wang, Wei Cheng, Wangjun Wu, Zengxiang Pan, Jinmin Lian, Honglin Liu, Yi-Liang Miao
The nucleosome, the fundamental structural unit of chromatin, is a critical regulator of gene expression. The mechanisms governing changes to nucleosome occupancy and positioning during somatic cell reprogramming remain poorly understood. We established a method for generating genome-wide nucleosome maps of porcine embryonic fibroblasts (PEF), reconstructed 1-cell embryos generated by somatic cell nuclear transfer (SCNT), and fertilized zygotes (FZ) using MNase sequencing with only 1,000 cells. We found that donor PEF chromatin, especially X chromosome, became more open after transfer into porcine oocytes and nucleosome occupancy decreased in promoters but increased in the genic regions...
July 3, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28687624/patellins-are-regulators-of-auxin-mediated-pin1-relocation-and-plant-development-in-arabidopsis-thaliana
#18
Ricardo Tejos, Cecilia Rodriguez-Furlan, Maciej Adamowski, Michael Sauer, Lorena Norambuena, Jiří Friml
Coordinated cell polarization in developing tissues is a recurrent theme in multicellular organisms. In plants, a directional distribution of the plant hormone auxin is at the core of many developmental programs. A feedback regulation of auxin on the polarized localization of PIN auxin transporters in individual cells has been proposed as a self-organizing mechanism for coordinated tissue polarization, but the molecular mechanisms linking auxin signalling to PIN-dependent auxin transport remain unknown. We performed a microarray-based approach to find regulators of the auxin-induced PIN relocation in the Arabidopsis thaliana root...
July 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28687208/assisted-reproduction-techniques-in-latin-america-the-latin-american-registry-2014
#19
Fernando Zegers-Hochschild, Juan Enrique Schwarze, Javier Crosby, Carolina Musri, Maria Teresa Urbina
Multinational data on assisted reproduction techniques (IVF and intractytoplasmic sperm injection [ICSI], frozen embryo transfer, oocyte donation, preimplantation genetic diagnosis and fertility preservation) were collected from 159 institutions in 15 Latin American countries. A total of 41.34% of IVF-ICSI cycles were conducted in women aged 35-39 years and 23.35% in women aged 40 years and older. After removing freeze-all cases, delivery rate per oocyte retrieval was 25.05% for ICSI and 27.41% for IVF. Multiple births included 20...
June 21, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28687207/high-risk-of-preterm-birth-and-low-birth-weight-after-oocyte-donation-ivf-analysis-of-133-785-live-births
#20
Mohan Shashikant Kamath, Belavendra Antonisamy, Mariano Mascarenhas, Sesh Kamal Sunkara
A higher risk of pregnancy complications occurs after assisted reproductive techniques compared with spontaneously conceived pregnancies. This is attributed to the underlying infertility and assisted reproduction technique procedures involved during treatment. It is a matter of interest whether use of donor oocytes affects perinatal outcomes compared with pregnancies after autologous IVF. Anonymized data were obtained from the Human Fertilization and Embryology Authority. The analysis included 5929 oocyte donation and 127,856 autologous IVF live births...
June 22, 2017: Reproductive Biomedicine Online
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