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Embryo transfer

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https://www.readbyqxmd.com/read/27907899/reducing-the-number-of-fetuses-in-a-pregnancy-providers-and-patients-views-of-challenges
#1
Robert L Klitzman
STUDY QUESTION: How do patients and providers perceive and make decisions about possible reductions of multi-fetal pregnancies? SUMMARY ANSWER: Physicians may be transferring additional embryos, assuming that patients will later undergo reduction if need be; but decisions to reduce pregnancies are difficult for patients, who may agree to undergo the procedure in advance and later renege. WHAT IS ALREADY KNOWN: Implanting more than one embryo increases the likelihood that at least one embryo will successfully lead to a child but also that the patient may end up with twins or higher-order multiple births...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27907898/obstetrical-and-perinatal-outcomes-following-blastocyst-transfer-compared-to-cleavage-transfer-a-systematic-review-and-meta-analysis
#2
W P Martins, C O Nastri, L Rienzi, S Z van der Poel, C R Gracia, C Racowsky
STUDY QUESTION: Is blastocyst transfer safe when compared to cleavage stage embryo transfer regarding obstetric and perinatal outcomes? SUMMARY ANSWER: The clinical equipoise between blastocyst and cleavage stage embryo transfer remains as the evidence associating blastocyst transfer with some adverse perinatal outcomes is of low/very low quality. WHAT IS KNOWN ALREADY: Extended embryo culture to the blastocyst stage provides some theoretical advantages and disadvantages...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27907897/cost-effectiveness-of-embryo-transfer-strategies-a-decision-analytic-model-using-long-term-costs-and-consequences-of-singletons-and-multiples-born-as-a-consequence-of-ivf
#3
M M J van Heesch, A D I van Asselt, J L H Evers, M A H B M van der Hoeven, J C M Dumoulin, C E M van Beijsterveldt, G J Bonsel, R H M Dykgraaf, J B van Goudoever, C Koopman-Esseboom, W L D M Nelen, K Steiner, P Tamminga, N Tonch, H L Torrance, C D Dirksen
STUDY QUESTION: What is the cost-effectiveness of elective single embryo transfer (eSET) versus double embryo transfer (DET) strategies from a societal perspective, when applying a time horizon of 1, 5 and 18 years? SUMMARY ANSWER: From a short-term perspective (1 year) it is cost-effective to replace DET with single embryo transfer; however when intermediate- (5 years) and long-term (18 years) costs and consequences are incorporated, DET becomes the most cost-effective strategy, given a ceiling ratio of €20 000 per quality-adjusted life years (QALY) gained...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27907016/direct-unequal-cleavages-embryo-developmental-competence-genetic-constitution-and-clinical-outcome
#4
Qiansheng Zhan, Zhen Ye, Robert Clarke, Zev Rosenwaks, Nikica Zaninovic
OBJECTIVE: To investigate the prevalence, developmental potential, chromosomal constitution and clinical outcome of embryos with direct unequal cleavages (DUC). DESIGN: A retrospective observational study. SETTING: Academic Institution. PARTICIPANT: 21,261 embryos from 3,155 cycles cultured in EmbryoScope®. RESULTS: The total incidence of DUCs per embryo occupying the first three cleavages were 26...
2016: PloS One
https://www.readbyqxmd.com/read/27906585/birth-of-cloned-microminipigs-derived-from-somatic-cell-nuclear-transfer-embryos-that-have-been-transiently-treated-with-valproic-acid
#5
Kazuchika Miyoshi, Hiroaki Kawaguchi, Kosuke Maeda, Masahiro Sato, Kohei Akioka, Michiko Noguchi, Masahisa Horiuchi, Akihide Tanimoto
In our previous study, we found that treatment of miniature pig somatic cell nuclear transfer (SCNT) embryos with 4 mM valproic acid (VPA), a histone deacetylase inhibitor, for 48 hours after activation enhanced blastocyst formation rate and octamer-binding transcription factor-3/4 (Oct-3/4) gene expression at the late blastocyst stage; however, the production of viable cloned pups failed, when those VPA-treated SCNT embryos were transferred to recipients. This failure suggests that the present VPA treatment is suboptimal...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27906583/isolation-of-bovine-skin-derived-precursor-cells-and-their-developmental-potential-after-nuclear-transfer
#6
Jiajia Xiao, Qiaoqiao Li, Pengxiang Qu, Zihan Zhang, Shaohui Pan, Yongsheng Wang, Yong Zhang
Nuclei from less differentiated stem cells yield high cloning efficiency. However, pluripotent stem cells are rather difficult to obtain from bovines. Skin-derived precursor (SKPs) cells exhibit a certain degree of pluripotency, which has been shown to enhance the efficiency of nuclear transfer (NT) in pigs. In this study, bovine SKPs were isolated and characterized. Results showed that bovine SKPs expressed nestin, fibronectin, vimentin, pluripotency-related genes, and characteristic neural crest markers, such as NGFR, PAX3, SOX9, SNAI2, and OCT4...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27901279/dopamine-agonists-for-preventing-ovarian-hyperstimulation-syndrome
#7
REVIEW
Huilin Tang, Selma Mourad, Suo-Di Zhai, Roger J Hart
BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is a potentially serious complication of ovarian stimulation in assisted reproduction technology (ART). It is characterised by enlarged ovaries and an acute fluid shift from the intravascular space to the third space, resulting in bloating, increased risk of venous thromboembolism and decreased organ perfusion. Most cases are mild, but forms of moderate or severe OHSS appear in 3% to 8% of in vitro fertilisation (IVF) cycles. The dopamine agonist cabergoline was introduced as a secondary prevention intervention for OHSS in women at high risk of OHSS undergoing ART treatment...
November 30, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27900886/premature-and-multiple-births-in-ivf-are-associated-with-pretreatment-circulating-lh-hcg-receptor-concentration
#8
Anne Chambers, Craig Fairbairn, Marco Gaudoin, Walter Mills, Subhasis Banerjee
The luteinizing hormone (LH) and pregnancy hormone, human chorionic gonadotrophin (hCG), share a common receptor: LH/hCG-R or LHCGR. In this prospective study involving 290 patients undergoing in vitro fertilization (IVF) and embryo transfer, we have examined whether pretreatment circulating LHCGR (sLHCGR) influences the course of pregnancy and perinatal outcome after embryo transfer. The blood samples were collected before the fertility treatment began and sLHCGR concentrations were measured using an enzyme-linked immunosorbent assay (ELISA) test...
November 30, 2016: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/27900612/early-human-embryos-are-naturally-aneuploid-can-that-be-corrected
#9
REVIEW
Amy Lee, Ann A Kiessling
Aneuploidy is common and may be a natural occurrence in early human embryos. Selecting against embryos containing aneuploid cells for embryo transfer has been reported to increase clinical pregnancies per transfer in some studies, but not others. Some aneuploidy is due to misallocation of chromosomes during meiosis, in either the egg or sperm, but most aneuploidy is due to misallocation of chromosomes during mitoses after fertilization. Big questions are as follows: Why does this happen? How much aneuploidy in a preimplantation embryo is compatible with normal fetal development? Is aneuploidy increased by in vitro culture, and/or could it be prevented or corrected in the IVF lab?...
November 29, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27899313/prognostic-factors-for-assisted-reproductive-technology-in-women-with-endometriosis-related-infertility
#10
Chloé Maignien, Pietro Santulli, Vanessa Gayet, Marie-Christine Lafay-Pillet, Diane Korb, Mathilde Bourdon, Louis Marcellin, Dominique de Ziegler, Charles Chapron
BACKGROUND: Assisted reproductive technology is one of the therapeutic options offered for managing endometriosis-associated infertility. Yet, published data on assisted reproductive technology outcome in women affected by endometriosis are conflicting and the determinant factors for pregnancy chances unclear. OBJECTIVE: To evaluate assisted reproductive technology outcomes in a series of 359 endometriosis patients, to identify prognostic factors and determine if there is an impact of the endometriosis phenotype...
November 26, 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27894281/successful-selective-reduction-of-a-heterotopic-cesarean-scar-pregnancy-in-the-second-trimester-a-case-report-and-review-of-the-literature
#11
Haiyan Yu, Hong Luo, Fumin Zhao, Xinghui Liu, Xiaodong Wang
BACKGROUND: Heterotopic cesarean scar pregnancy is a cesarean scar pregnancy combined with an intrauterine pregnancy that predisposes a woman to life-threatening complications such as uterine rupture and massive bleeding. Preservation of the intrauterine pregnancy in heterotopic cesarean scar pregnancy is a great challenge. CASE PRESENTATION: We report a case of a 33-year-old woman with heterotopic cesarean scar pregnancy after IVF-embryo transfer (ET). Expectant management was carried out with early diagnosis of heterotopic cesarean scar pregnancy (HCSP), and selective fetal reduction of cesarean scar pregnancy (CSP) was performed by ultrasound-guided intrathoracic injection of potassium chloride (KCl) at 16 + 4 weeks of gestation due to aggravation of CSP...
November 29, 2016: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/27894197/temporal-validation-of-a-simplified-blastocyst-grading-system
#12
Alison Richardson, Sophie Brearley, Saran Ahitan, Sarah Chamberlain, Tracey Davey, Lyndsey Zujovic, James Hopkisson, Bruce Campbell, Nick Raine-Fenning
We have recently established the clinical effectiveness and credibility of a simplified blastocyst grading system by demonstrating its prognostic potential and the inter- and intra-observer variability associated with it. To be considered clinically useful, however, the grading system also needs to be accurate (i.e. well calibrated with good discriminative ability). This study prospectively evaluates the performance of the grading system on subsequent patients from the same IVF unit in an attempt to temporally validate the model...
November 28, 2016: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/27893658/freeze-all-embryo-transfer-awareness-and-acceptance-of-ivf-et-patients-in-china
#13
Yan-Xiu Guo, Yan-Jing Yin, Li Tian
The aim of the study was to determine the level of awareness and acceptance of in vitro fertilization pre-embryo transfer (IVF-ET) patients for freeze-all embryo transfer, improve relevant technical specifications, and optimize treatment results based on medical experience.Questionnaires were completed by women who received in vitro fertilization embryo transfer. A total of 377 valid samples were included into this study. Through focus group discussions and tablet assisted intercept interviews, we analyzed the basic situation, the awareness for frozen-all embryo transfer, and the need for frozen-all embryo transfer information for infertile patients...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27890045/protein-in-culture-and-endogenous-lipid-interact-with-embryonic-stages-in-vitro-to-alter-calf-birthweight-after-embryo-vitrification-and-warming
#14
E Gómez, S Carrocera, S Uzbekova, D Martín, A Murillo, M Alonso-Guervós, F Goyache, M Muñoz
Short-term protein removal in vitro improves long-term blastocyst competence to survive vitrification. We investigated the mechanisms and effects underlying protein removal. Day-6 morulae and early blastocysts were cultured individually with and without protein for 24h. Development and lipid content were analysed in expanded blastocysts derived from morulae (M-XB) and from early blastocysts (EB-XB). Expression of genes involved in lipid metabolism, stress responses and apoptosis was analysed in fresh and vitrified-warmed M-XB produced with and without protein...
November 28, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27889867/the-impact-of-younger-age-on-treatment-discontinuation-in-insured-ivf-patients
#15
Laura E Dodge, Denny Sakkas, Michele R Hacker, Rachael Feuerstein, Alice D Domar
PURPOSE: This retrospective cohort study aimed to determine whether age influences treatment discontinuation among insured patients undergoing in vitro fertilization (IVF). We hypothesized that the youngest patients would be the least likely to discontinue treatment. METHODS: All women age 18-42 who underwent their first fresh, non-donor IVF cycle from 2002 to 2013 were followed until a live birth was achieved, until they discontinued treatment at our center (not presenting for treatment for a one-year period), or until they completed six fresh or frozen embryo transfer cycles, whichever occurred first...
November 26, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27889789/effect-of-natural-cycle-endometrial-preparation-for-frozen-thawed-embryo-transfer-in-patients-with-advanced-endometriosis
#16
Haiyan Guo, Yun Wang, Qiuju Chen, Weiran Chai, Qifeng Lv, Yanping Kuang
BACKGROUND The aim of this study was to investigate the effect of natural cycle (NC) endometrial preparation for frozen-thawed embryo transfer (FET) in women with advanced endometriosis. MATERIAL AND METHODS This retrospective study included 179 patients with stage III-IV endometriosis who underwent 233 FET cycles at a tertiary care academic reproductive medical center between March 2011 and August 2013 (group A). The control group included 258 patients with tubal factor infertility who underwent 300 FET cycles (group B)...
November 27, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27887711/low-body-mass-index-compromises-live-birth-rate-in-fresh-transfer-in%C3%A2-vitro-fertilization-cycles-a-retrospective-study-in-a-chinese-population
#17
Jiali Cai, Lanlan Liu, Junwen Zhang, Huiling Qiu, Xiaoming Jiang, Ping Li, Aiguo Sha, Jianzhi Ren
OBJECTIVE: To evaluate the effects of low body mass index (BMI) on in vitro fertilization (IVF) outcomes in fresh transfer cycles. DESIGN: Retrospective cohort study. SETTING: University-affiliated hospital. PATIENT(S): A total of 4,798 cycles with conventional stimulation and fresh transfer in a single IVF center during the period 2013-2014. Low BMI (<18.5 kg/m(2)) was defined according to World Health Organization guidelines, and cycles within a normal weight range (18...
November 22, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27884753/cell-free-dna-induced-apoptosis-of-granulosa-cells-by-oxidative-stress
#18
Yichun Guan, Wenjuan Zhang, Xingling Wang, Pengfei Cai, Qi Jia, Wenjie Zhao
BACKGROUND: Cell-free DNA is a DNA fragment that is produced by cell apoptosis which can affect the micro-environment of cell apoptosis. The levels of Cell-free DNA have been associated with successful rate of in vitro fertilization-embryo transfer (IVF-ET) and embryonic development. Our aim is to determine the relationship between cell-free DNA and embryo quality. The mechanisms of cell-free DNA in granulose and the apoptosis will be determined also. METHODS: The study enrolled patients who were undergone IVF for the first time and grouped the patients as pregnant (n=130) and non-pregnant (n=59)...
November 21, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27882628/the-influence-of-testicular-microlithiasis-on-the-outcomes-of-in-vitro-fertilisation-in-a-chinese-han-population
#19
C Xu, F-F Zhang, H-L Yang, G Ma, B Zhang, K-J Li, T-J Zhang, J-L Liu, X-J Xuan
To investigate the influence of testicular microlithiasis (TM) on the outcomes of in vitro fertilisation (IVF), we retrospectively reviewed the ultrasonography results of the TM patients who underwent IVF treatment in our hospital. They were classified as classic-TM (CTM) or limited-TM (LTM) comparing with patients without TM (Non-TM). Semen parameters, rates of fertilisation, normal fertilisation, good embryos and clinical pregnancy were analysed. The Non-TM group showed higher percentage of morphologically normal spermatozoa than the CTM or LTM group (4...
November 23, 2016: Andrologia
https://www.readbyqxmd.com/read/27881336/-outcome-analysis-of-monozygotic-twin-pregnancy-conceived-by-assisted-reproductive-techniques
#20
Shu-Xian Feng, Xue-Lan Li, Xing-Yu Zhou, Yu-Dong Liu, Ping-Ping Guo, De-Sheng Ye, Xin Chen, Shi-Ling Chen
OBJECTIVE: To analyze the incidence, management, and outcomes of monozygotic twin (MZT) pregnancy conceived by assisted reproductive techniques (ART). METHODS: A retrospective analysis was performed of clinical pregnancies after in vitro fertilization and embryo transfer (IVF-ET) and introcytoplasmic sperm injection and embryo transfer (ICSI-ET) from January, 2010 to June 2015 at our center. We investigated the incidence, managements and outcomes of 94 MZT pregnancies...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
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