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Embryo transfer

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https://www.readbyqxmd.com/read/28548200/live-birth-after-transfer-of-vitrified-warmed-embryo-derived-from-vitrified-warmed-oocyte-and-frozen-thawed-sperm-following-failed-icsi-case-report
#1
Zhihong Zhang, Jihong Zhu, Hongyang Zhang, Qi Xi, Dongfeng Geng, Shu Deng, Ruizhi Liu
Since the first successful pregnancy from a frozen human oocyte was reported, remarkable technological progress has been made in the area of cryopreservation of human oocytes. We report a successful delivery of two healthy babies after transfer of vitrified-warmed embryos derived from intracytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm. A female patient and her husband with severe oligoasthenspermia are reported. At the day of oocyte collection, very few inactive sperms were found in her husband semen...
March 2017: Revista Médica de Chile
https://www.readbyqxmd.com/read/28545306/developmental-potential-of-embryos-from-cycles-containing-oocytes-with-severe-ovoid-zona-pellucida
#2
Shun Xiong, Wei Han, Weiwei Liu, Lihong Wu, Jun Xia Liu, Yang Gao, Guoning Huang
The purpose of this study was to determine the incidence of oocytes with severe ovoid zona pellucida (ZP), investigate the development potential of their sibling oocytes and the clinical outcomes from affected cycles. The data were collected from our medical records. Cycles having at least one oocyte with severe ovoid ZP were defined as the 'severe ovoid group', cycles having at least one oocyte with mild ovoid ZP were defined as the 'mild ovoid group', whereas cycles without oocytes with ovoid ZPs were defined as the 'control group' (n = 150 for each group)...
May 26, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28545049/first-cloned-bactrian-camel-camelus-bactrianus-calf-produced-by-interspecies-somatic-cell-nuclear-transfer-a-step-towards-preserving-the-critically-endangered-wild-bactrian-camels
#3
Nisar Ahmad Wani, Binoy S Vettical, Seung B Hong
Studies were conducted to explore the possibility of employing dromedary camel (Camelus dromedarius) oocytes as recipient cytoplasts for the development of interspecies somatic cell nuclear transfer (iSCNT) embryos using skin fibroblast cells of an adult Bactrian camel (Camelus bactrianus) and Llama (Llama glama) as donor nuclei. Also, the embryos reconstructed with Bactrian cells were transferred into the uterus of synchronized dromedary camel recipients to explore the possibility of using them as surrogate mothers...
2017: PloS One
https://www.readbyqxmd.com/read/28544972/the-effect-of-elevated-progesterone-levels-before-oocyte-retrieval-in-women-undergoing-ovarian-stimulation-for-ivf-treatment-on-the-genomic-profile-of-peri-implantation-endometrium
#4
Liu Liu, Jin Huang, Tin Chiu Li, Xu Tao Hong, Susan Laird, Yong Dong Dai, Xiao Mei Tong, Hai Yan Zhu, Songying Zhang
To evaluate the effects of high progesterone prior to oocyte retrieval on the genomic profile of peri-implantation endometrium, we conducted this single-center, prospective cohort study. Depending on whether or not the progesterone level on the day of hCG administration and the day after hCG administration were elevated, a total of 20 women undergoing IVF treatment who did not have fresh embryo transfer were included: Group 1 refers to subjects with normal progesterone level on both days; Group 2 refers to subjects with normal progesterone level on the day of hCG administration and high progesterone level on the day after hCG administration; Group 3 refers to subjects with high progesterone level on the day of hCG administration and normal progesterone level on the day after hCG administration; Group 4 refers to subjects with high progesterone level on both days...
May 17, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/28542142/deletion-of-pdcd5-in-mice-led-to-the-deficiency-of-placenta-development-and-embryonic-lethality
#5
Ge Li, Chentong Xu, Xin Lin, Liujing Qu, Dan Xia, Beiqi Hongdu, Yan Xia, Xiaokun Wang, Yaxin Lou, Qihua He, Dalong Ma, Yingyu Chen
Programmed cell death 5 (PDCD5) is an apoptosis promoter molecule that displays multiple biological activities. However, the function of PDCD5 in vivo has not yet been investigated. Here, we generated a Pdcd5 knockout mouse model to study the physiological role of PDCD5 in vivo. Knockout of the Pdcd5 gene resulted in embryonic lethality at mid-gestation. Histopathological analysis revealed dysplasia in both the LZs and JZs in Pdcd5(-/-) placentas with defects in spongiotrophoblasts and trophoblast giant cells...
May 25, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28540574/diagnosis-and-management-of-heterotopic-pregnancy-following-embryo-transfer-clinical-analysis-of-55-cases-from-a-single-institution
#6
Jiangtao Lyu, Hong Ye, Weihua Wang, Yi Lin, Wenjie Sun, Li Lei, Lijuan Hao
OBJECTIVE: The aims of this study were to summarize the clinical features of patients with heterotopic pregnancy (HP) following embryo transfer (ET) and explore the risk factors for miscarriage after surgery. METHODS: All patients with HP following ET treated by surgery between August 2014 and August 2015 in Chongqing Health Center for Women and Children were retrospectively reviewed. RESULTS: Fifty-five patients were identified, including 40 with tubal HP, 9 interstitial HP and 6 cornual HP...
May 24, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28540311/impacts-of-anti-dsdna-antibody-on-in-vitro-fertilization-embryo-transfer-and-frozen-thawed-embryo-transfer
#7
Jiao Fan, Yiping Zhong, Cuina Chen
Our purpose is to explore whether anti-dsDNA antibody, which was demonstrated to enter living cells and induced apoptosis, could adversely affect reproductive outcomes. A total of 259 women receiving the in vitro fertilization-embryo transfer (IVF) cycle were enrolled in this study, including 52 women with positive ANA and anti-dsDNA (ANA+/anti-dsDNA+ group), 86 women with positive ANA and negative anti-dsDNA (ANA+/anti-dsDNA- group), and 121 women with negative ANA and anti-dsDNA (ANA-/anti-dsDNA- group). 136 nonpregnant women among 259 patients in the IVF-ET cycle were enrolled in the hormone replacement therapy frozen-thawed embryo transfer (HRT-TET) cycle...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28536295/papers-of-note-in-science356-6339
#8
Annalisa M VanHook
This week's articles identify a repulsive guidance system that controls the recruitment of T cells to germinal centers; a mechanism for controlling the transfer of nutrients from the placenta to early mouse embryos; and the subcellular location of calcium dynamics in astrocytes.
May 23, 2017: Science Signaling
https://www.readbyqxmd.com/read/28534229/production-of-a-gonadotropin-releasing-hormone-2-receptor-knockdown-gnrhr2-kd-swine-line
#9
A T Desaulniers, R A Cederberg, G A Mills, C A Lents, B R White
Swine are the only livestock species that produce both the second mammalian isoform of gonadotropin-releasing hormone (GNRH2) and its receptor (GNRHR2). Previously, we reported that GNRH2 and GNRHR2 mediate LH-independent testosterone secretion from porcine testes. To further explore this ligand-receptor complex, a pig model with reduced GNRHR2 expression was developed. Small hairpin RNA sequences targeting porcine GNRHR2 were subcloned into a lentiviral-based vector, lentiviral particles were generated and microinjected into the perivitelline space of zygotes, and embryos were transferred into a recipient...
May 22, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28533904/-this-is-where-it-all-started-the-pivotal-role-of-plc%C3%AE-within-the-sophisticated-process-of-mammalian-reproduction-a-systemic-review
#10
Itai Gat, Raoul Orvieto
Mammalian reproduction is one of the most complex and fascinating biological phenomenon, which aims to transfer maternal and paternal genetic material to the next generation. At the end of oogenesis and spermatogenesis, both haploid gametes contain a single set of chromosomes ready to form the zygote, the first cell of the newly developing individual. The mature oocyte and spermatozoa remain in a quiescent state, during which the oocyte is characterized by nuclear and cytoplasmic arrest, while the spermatozoa necessitates further maturation within the epididymis and female reproductive track prior to egg fertilization...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28532827/hepatoma-derived-growth-factor-protein-quantification-in-uterine-fluid-gene-expression-in-endometrial-cell-culture-and-effects-on-in%C3%A2-vitro-embryo-development-pregnancy-and-birth
#11
E Gómez, S Carrocera, D Martin, M J Sánchez-Calabuig, A Gutiérrez-Adán, A Murillo, M Muñoz
Hepatoma-derived growth factor (HDGF) is present in the endometrium of cows and other mammals. Recombinant HDGF (rHDGF) improves bovine blastocyst development in vitro. However, specific culture conditions and essential aspects of HDGF uterine physiology are yet unknown. In this work we quantified total HDGF protein in uterine fluid (UF) by multiple reaction monitoring (MRM), and analyzed effects of rHDGF on specific embryonic stages with Day-6 bovine embryos cultured in vitro with and without BSA, and on pregnancy viability and calf phenotypes after embryo transfer to recipients...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28531369/should-high-bmi-be-a-reason-for-ivf-treatment-denial
#12
S Friedler, O Cohen, G Liberty, B Saar-Ryss, S Meltzer, T Lazer
Our aim was to examine the influence of BMI on the live-birth rate following IVF/ICSI and evaluate its specific contribution among other factors thus enabling accurate reproductive policy development. All patients that underwent IVF/ICSI at our center during January 2012-July 2015 were included in this retrospective study. A total of 1654 ICSI cycles were divided into four groups according to the patient's BMI (kg/m(2)): group I (normal weight): <25 (943 cycles); group II (overweight): 25-30 (403 cycles); group III (obese): 30-35 (212 cycles); group IV (morbid obesity): >35 (96 cycles)...
May 20, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28529323/an-immunogenic-phenotype-in-paternal-antigen-specific-cd8-t-cells-at-embryo-implantation-elicits-later-fetal-loss-in-mice
#13
Lachlan M Moldenhauer, Kerrilyn R Diener, John D Hayball, Sarah A Robertson
Central to pregnancy success is a state of T cell tolerance to paternal antigens, which is initiated at conception. The role and regulation of specific phenotypes of CD8(+) T cells in mediating pregnancy tolerance is not clear. This study aimed to investigate the impact on pregnancy outcome of altering the cytokine environment during maternal CD8(+) T cell priming in early pregnancy. Transgenic Act-mOVA male mice were mated to C57BL/6 (B6) females to generate fetuses expressing ovalbumin (OVA) as a model paternal antigen...
May 22, 2017: Immunology and Cell Biology
https://www.readbyqxmd.com/read/28527625/cosmetic-micromanipulation-of-vitrified-warmed-cleavage-stage-embryos-does-not-improve-art-outcomes-an-ultrastructural-study-of-fragments
#14
Somayyeh Safari, Mohammad Ali Khalili, Zeinab Barekati, Iman Halvaei, Morteza Anvari, Stefania A Nottola
The aim was to study the ultrastructure of cytoplasmic fragments along with the effect of cosmetic micromanipulation (CM) on the morphology and development of vitrified-warmed embryos as well as assisted reproductive technology (ART) outcomes. A total of 96 frozen embryo transfer (FET) cycles were included in this prospective randomized study. They were divided into three groups of CM (n=32), sham (n=32) and control (n=32). In the CM group, the vitrified- warmed embryos were subjected to fragments and coarse granules removal (cosmetic micromanipulation) after laser assisted zona hatching (LAH); sham group subjected only to LAH and no intervention was taken for the control group...
May 17, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28525345/in-vitro-production-of-sudanese-camel-camelus-dromedarius-embryos-from-epididymal-spermatozoa-and-follicular-oocytes-of-slaughtered-animals
#15
A E Abdelkhalek, Sh A Gabr, W A Khalil, Sh M Shamiah, L Pan, G Qin, M H Farouk
Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter)...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28523429/kir-lilrb-and-their-ligands-genes-as-potential-biomarkers-in-recurrent-implantation-failure
#16
REVIEW
Izabela Nowak, Karolina Wilczyńska, Jacek R Wilczyński, Andrzej Malinowski, Paweł Radwan, Michał Radwan, Piotr Kuśnierczyk
Reproductive failure in humans is a very important social and economic problem, because nowadays women decide to conceive later in life and delay motherhood. Unfortunately, with increasing age they have less chance for natural fertilization and maintenance of pregnancy. Many of them need assisted reproductive technology. Approximately 10% of women after in vitro fertilization-embryo transfers experience recurrent implantation failure (RIF). Multiple factors may contribute to RIF, including oocyte and sperm quality, parental chromosomal anomalies, genetic or metabolic abnormalities of the embryo, poor uterine receptivity, immunological disturbances in the implantation site, and some gynecologic pathologies such as endometriosis, uterine fibroids, hydrosalpinx and endometrial polyps...
May 18, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28523409/factors-associated-with-a-poor-prognosis-for-the-ivf-icsi-live-birth-rate-in-women-with-rafs-stage-iii-and-iv-endometriosis
#17
Pauline Roux, Jeanne Perrin, Julien Mancini, Aubert Agostini, Léon Boubli, Blandine Courbiere
PURPOSE: To assess the factors associated with a poor prognosis for a cumulative IVF live birth rate (LBR) in women with stage III and IV endometriosis according to the revised classification of the American Fertility Society (rAFS). METHODS: A retrospective cohort study was conducted between January 1, 2010, and December 31, 2014, in our Reproductive Medicine Center. We analyzed different factors associated with a poor prognosis for a cumulative IVF LBR in women with rAFS stage III and IV endometriosis...
May 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28520729/top-quality-blastocyst-formation-rates-in-relation-to-progesterone-levels-on-the-day-of-oocyte-maturation-in-gnrh-antagonist-ivf-icsi-cycles
#18
V S Vanni, E Somigliana, M Reschini, L Pagliardini, E Marotta, S Faulisi, A Paffoni, P Vigano', W Vegetti, M Candiani, E Papaleo
Cycles with progesterone elevation during controlled ovarian stimulation (COS) for IVF/ICSI are commonly managed with a "freeze-all" strategy, due to a well-recognized detrimental effect of high progesterone levels on endometrial receptivity. However, also a detrimental effect of elevated progesterone on day-3 embryo quality has recently been found with regards to top quality embryo formation rate. Because blastocyst culture and cryopreservation are largely adopted, we deemed relevant to determine whether this detrimental effect is also seen on blastocyst quality on day 5-6...
2017: PloS One
https://www.readbyqxmd.com/read/28516762/organophosphorus-flame-retardants-in-pregnant-women-and-their-transfer-to-chorionic-villi
#19
Fanrong Zhao, Mo Chen, Fumei Gao, Huan Shen, Jianying Hu
The potential for prenatal exposure has recently raised concerns over the health risks of endocrine disruptors; however, knowledge about human prenatal exposure to organophosphorus flame retardants (OPFRs) is lacking. In this study, 2-ethylhexyl diphenyl phosphate (EHDPP), tributyl phosphate (TBP), triphenyl phosphate (TPHP), and tris(2-chloroethyl) phosphate (TCEP) were detected in the majority of chorionic villus samples, with median concentrations of 13.6, 18.8, 11.1, and 0.51 ng/g dry weight (dw), respectively, significantly higher than those in the matching maternal decidua samples (5...
May 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28512301/maternal-antioxidant-provisioning-mitigates-pollutant-induced-oxidative-damage-in-embryos-of-the-temperate-sea-urchin-evechinus-chloroticus
#20
Kathryn N Lister, Miles D Lamare, David J Burritt
One mechanism of pollution resistance in marine populations is through transgenerational plasticity, whereby offspring capacity to resist pollution reflects parental exposure history. Our study aimed to establish correlations between oxidative stress biomarkers and key reproductive fitness parameters in the temperate sea urchin Evechinus chloroticus following exposure to dietary polycyclic aromatic hydrocarbons (PAHs). PAH-exposed adults exhibited total gonad tissue concentrations of PAHs in excess of 4 and 5 times baseline levels, for females and males respectively...
May 16, 2017: Scientific Reports
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