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Embryo transfer

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https://www.readbyqxmd.com/read/29684629/mid-luteal-phase-gonadotropin-releasing-hormone-agonist-support-in-frozen-thawed-embryo-transfers-during-artificial-cycles-a-prospective-interventional-pilot-study
#1
Jaana Seikkula, Katja Ahinko, Päivi Polo-Kantola, Leena Anttila, Saija Hurme, Helena Tinkanen, Varpu Jokimaa
OBJECTIVE: To assess the effect of an additional single mid-luteal dose of gonadotropin-releasing hormone agonist (GnRHa) on pregnancy and perinatal outcomes in hormonally substituted frozen embryo transfer (FET) cycles. STUDY DESIGN: A prospective interventional pilot study. Women scheduled for FET were randomly selected to receive standard hormonal replacement therapy (HRT) for endometrial preparation or HRT with a single additional subcutaneous dose of 0.1mg triptorelin at the time of implantation...
April 20, 2018: Journal of Gynecology Obstetrics and Human Reproduction
https://www.readbyqxmd.com/read/29684167/reproduction-in-domestic-ruminants-during-the-past-50-years-discovery-to-application
#2
Michael F Smith, Rodney D Geisert, John J Parrish
The study of reproductive physiology in domestic ruminants has progressed from the whole animal to the molecular level in an amazingly short period of time. The volume of information on this subject is enormous; therefore, we have focused on domestic ruminants, with an emphasis on cattle. To date, artificial insemination (AI) is perhaps the most powerful technique that reproductive physiologists and geneticists have provided the livestock industry for genetic improvement. Early efforts to establish AI as a tool were initiated in Russia around 1899 and since that time major advances in methods of semen collection, evaluation of male fertility, cryopreservation of sperm, sex-sorted semen, and estrous cycle control have occurred...
April 19, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29683366/predictive-value-of-basal-androgen-levels-on-ongoing-pregnancy-rates-during-in-vitro-fertilization-cycles
#3
Dan Pan, Juanzi Shi, Hanying Zhou, Na Li, Pengfei Qu
We aimed to evaluate the impact of elevated basal androgen levels on the endometrial receptivity. This study retrospectively enrolled 5278 fresh in vitro fertilization (IVF) cycles and sought to determine whether increased basal androgen levels are associated with adverse outcomes in regard to ongoing pregnancy rates. The results showed that the average age of our sample was 29.31 years. Almost 61.6% of all embryo transfers were with Day 3 embryos and the remaining 38.4% were with Day 5 embryos. The ongoing pregnancy rate was 56...
April 23, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29681919/efficient-gene-transfer-and-gene-editing-in-sterlet-acipenser-ruthenus
#4
Ji Chen, Wei Wang, Zhaohui Tian, Ying Dong, Tian Dong, Hua Zhu, Zuoyan Zhu, Hongxia Hu, Wei Hu
The sturgeon (Acipenseriformes) is an important farmed species because of its economical value. However, neither gene transfer nor gene editing techniques have been established in sturgeon for molecular breeding and gene functional study until now. In this study, we accomplished gene transfer and gene editing in sterlet ( Acipenser ruthenus ), which has the shortest sexual maturation period of sturgeons. The plasmid encoding enhanced green fluorescent protein (EGFP) was transferred into the embryos of sterlet at injection concentration of 100 ng/μL, under which condition high survival rate and gene transfer rate could be achieved...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29681721/progesterone-hypersensitivity-a-challenge-for-luteal-support
#5
Astha Gupta, Deepak Goenka, Mohan L Goenka
Progesterone hypersensitivity is a rare phenomenon which can occur after both endogenous and exogenous exposures. We present a case of hypersensitivity to various forms and routes of exogenous progesterone. A 27-year-old female presented with primary infertility. Investigations revealed Grade 1 endometriosis and polycystic ovary syndrome. Three cycles of intrauterine insemination were attempted which were unsuccessful and in vitro fertilization was proceeded. Six blastocysts of Grade A were formed and cryopreserved...
January 2018: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29681720/live-birth-after-rescue-in-vitro-maturation-intracytoplasmic-sperm-injection-in-type-1-diabetes-polycystic-ovary-syndrome-patient-using-clomiphene-antagonist-protocol
#6
Samundi Sankari, M Elanchezhian, Divya Selvamani, M Nagarajan, D Gopikrishnan
Infertility in patients with polycystic ovary syndrome (PCOS) associated with diabetes leads to challenging situations seeking alternative treatments. In vitro maturation (IVM) followed by intracytoplasmic sperm injection (ICSI) could overcome the challenges with promising pregnancies in such patients. In the treatment of a 32-year-old diabetic woman who also had PCOS, single immature oocyte was retrieved. Rescue IVM followed by ICSI yielded a grade 1 day 3 embryo which on transferring resulted in pregnancy and a healthy infant was delivered...
January 2018: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29681719/intracytoplasmic-sperm-injection-with-assisted-oocyte-activation-resulting-in-successful-pregnancies-and-live-birth-in-couples-with-globozoospermia-a-report-of-two-cases
#7
Puneet Kaur Kochhar, Pranay Ghosh
Globozoospermia, characterized by round-headed acrosomeless sperm, is a rare and severe form of teratozoospermia. We report a successful pregnancy in two cases of total globozoospermia after intracytoplasmic sperm injection (ICSI) with oocyte activation with calcium ionophore. In thefirst case, globozoospermia was diagnosed on the day of oocyte retrieval. Among 11 retrieved oocytes, only one fertilized after ICSI. The pregnancy test 2 weeks after embryo transfer was negative. Two months later, the patient underwent ovarian stimulation again...
January 2018: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29681715/can-intracytoplasmic-morphologically-selected-spermatozoa-injection-be-used-as-first-choice-of-treatment-for-severe-male-factor-infertility-patients
#8
Geeta Goswami, Meeta Sharma, Deepika Jugga, Devi M Gouri
Aim: This study was carried out to assess the outcome of the intracytoplasmic morphologically selected sperm injection (IMSI) technique compared with the previous failed intracytoplasmic sperm injection (ICSI) attempts in oligoasthenoteratozoospermia (OAT)/severe OAT (SOAT)/teratozoospermia patients. Setting: Institution-based, in vitro fertilization center. Study Design: It was a nonrandomized prospective study including 57 couples who had previous one or two ICSI failures (failure due to no implantation as embryos were transferred in these cycles and had no pregnancy) due to male factor...
January 2018: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29681712/effectiveness-of-anti-mullerian-hormone-tailored-protocol-compared-to-conventional-protocol-in-women-undergoing-in-vitro-fertilization-a-randomized-controlled-trial
#9
Sumi Thomas, Mousumi Acharya, K Muthukumar, Achamma Chandy, Mohan S Kamath, T K Aleyamma
Background: Assessment of ovarian reserve before an in vitro fertilization cycle (IVF) is one among the many factors that predicts a successful cycle. Individualized protocol based on ovarian reserve is designed to optimize the pregnancy outcome without compromising the patient safety. Although authors have shown that anti-Mullerian hormone-tailored (AMH) protocols have reduced the treatment burden and improved pregnancy rates, a few others have questioned its efficacy. Aims: The aim of this study was to decide whether the AMH-tailored protocol or the conventional protocol better decides IVF outcomes...
January 2018: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29680508/-birth-weight-and-frozen-embryo-transfer-state-of-the-art
#10
M Anav, A Ferrières-Hoa, A Gala, A Fournier, S Zaragoza, E Vintejoux, C Vincens, S Hamamah
The aim of this study was to update our acknowledgment if there is a link between assisted embryo cryopreservation and epigenetics in human? Animal studies have demonstrated epigenetics consequence and especially imprinting disorders due to in vitro culture. In human, it is important to note that after frozen embryo transfer birth weight is significantly increased by 81 to 250g. But these studies cannot identify the reasons of such difference. This review strongly suggests that embryo cryopreservation is responsible for birth weight variations but mechanisms not yet elucidated...
April 18, 2018: Gynecologie, Obstetrique, Fertilite & Senologie
https://www.readbyqxmd.com/read/29680315/luteal-phase-support-after-gonadotropin-releasing-hormone-agonist-triggering-does-it-still-matter
#11
REVIEW
Claudio Benadiva, Lawrence Engmann
Despite the increasing utilization of freeze-only IVF protocols, there is still a need for adequate management of the luteal phase after GnRH agonist trigger for patients who desire a fresh embryo transfer. Two approaches, intensive luteal support with E2 and P, and the use of adjuvant low-dose hCG either at the time of GnRH agonist trigger (dual trigger) or at the time of oocyte retrieval, have been shown to be effective in maintaining adequate pregnancy outcomes. The addition of low-dose hCG should be used with caution, because it may increase the risk of ovarian hyperstimulation syndrome...
April 18, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29679182/non-o-blood-group-and-outcomes-of-in-vitro-fertilization
#12
M Di Nisio, A Ponzano, G M Tiboni, M D Guglielmi, A W S Rutjes, E Porreca
PURPOSE: Retrospective and cross-sectional studies suggested that non-O blood group may be associated with failures of in vitro fertilization (IVF), but data remain controversial. The aim of this observational cohort study was to prospectively evaluate the effect of non-O blood type on clinical outcomes of IVF. METHODS: Women < 40 years who underwent IVF and had ABO blood type recorded as part of the routine workup were eligible. The primary study outcome was live birth...
April 20, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29678568/likelihood-of-pregnancy-after-the-transfer-of-embryos-derived-from-follicle-aspiration-and-in-vitro-embryo-production-sessions-with-different-relative-efficiencies
#13
Luiz Fernando Feres, Luiz Gustavo Bruno Siqueira, Miller Pereira Palhao, Lívia Loiola Dos Santos, Felipe Zandonadi Brandao, Joao Henrique Moreira Viana
The aim of the present study was to evaluate the likelihood of pregnancy of in vitro-produced (IVP) embryos from batches with distinct relative efficiencies. Data were retrospectively analyzed from 605 transvaginal ultrasonic-guided follicle aspiration sessions (OPU) followed by in vitro embryo production (IVEP) and 2456 fresh embryo transfers (ET), performed between 2008 and 2012 in individuals of the Gir (dairy Bos indicus) breed. The OPU and IVEP were performed using standard procedures by a single group of technicians at the same laboratory facility...
April 15, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29676585/modified-hmg-stimulated-an-effective-option-in-endometrial-preparation-for-frozen-thawed-embryo-transfer-in-patients-with-normal-menstrual-cycles
#14
Pinxiu Huang, Lihong Wei, Xinlin Li, Zhong Lin
To evaluate the clinical efficacy of modified human menopausal gonadotropin (hMG) stimulated, hormone replacement therapy (HRT), natural cycling and letrozole ovulation induction during endometrial preparation for frozen-thawed embryo transfer (FET) in patients with normal menstrual cycles. This retrospective analysis included a total of 5070 cycles of patients with normal menstrual patterns who underwent FET between October 2009 and September 2015. The patients were divided into four groups according to the method of endometrial preparation for FET: 1838 cycles were natural, 1666 underwent HRT, 340 underwent letrozole ovulation induction and 1226 underwent modified hMG stimulated...
April 20, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29675488/trans-dermal-estrogen-oestrogel-for-endometrial-preparation-in-freeze-embryo-transfer-cycle-an-rct
#15
Ensieh Shahrokh Tehraninejad, Roya Kabodmehri, Batol Hosein Rashidi, Mina Jafarabadi, Fateme Keikha, Masomeh Masomi, Fedieh Hagholahi
Background: Estrogen and progesterone are two crucial factors for endometrial preparation in frozen embryo transfer (FET) cycles. Studies assessing different forms of estradiol in FET have published already but literature lacks enough surveys on transdermal estrogen application in reproductive medicine. Objective: To investigate the effects of trans dermal estrogen (Oestrogel) on pregnancy rates in patients that candidate for FET cycle. Materials and Methods: In this randomized clinical trial, 100 women undergoing FET cycles referred to Imam Khomeeini Hospital were enrolled in two groups, randomly...
January 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29675483/fresh-versus-frozen-embryo-transfer-after-gonadotropin-releasing-hormone-agonist-trigger-in-gonadotropin-releasing-hormone-antagonist-cycles-among-high-responder-women-a-randomized-multi-center-study
#16
Abbas Aflatoonian, Mahnaz Mansoori-Torshizi, Maryam Farid Mojtahedi, Behrouz Aflatoonian, Mohammaad Ali Khalili, Mohammad Hossein Amir-Arjmand, Mehrdad Soleimani, Nastaran Aflatoonian, Homa Oskouian, Nasim Tabibnejad, Peter Humaidan
Background: The use of embryo cryopreservation excludes the possible detrimental effects of ovarian stimulation on the endometrium, and higher reproductive outcomes following this policy have been reported. Moreover, gonadotropin-releasing hormone agonist trigger in gonadotropin-releasing hormone (GnRH) antagonist cycles as a substitute for standard human chorionic gonadotropin trigger, minimizes the risk of ovarian hyperstimulation syndrome (OHSS) in fresh as well as frozen embryo transfer cycles (FET)...
January 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29673730/early-oocyte-denudation-does-not-compromise-icsi-cycle-outcome-a-large-retrospective-cohort-study
#17
Osama Naji, Natalie Moska, Yaser Dajani, Awatuf El-Shirif, Hassan El-Ashkar, Mohamed Hosni, Mohamed Khalil, Yacoub Khalaf, Virginia Bolton, Tarek El-Toukhy
This retrospective cohort study of 2051 consecutive fresh non-donor intracytoplasmic sperm injection (ICSI) cycles investigated whether time from oocyte retrieval to denudation, precisely measured and recorded by an operator-independent automated radiofrequency-based system, affected cycle outcome. ICSI cycles were divided into two groups: group I (denudation within <2 h of oocyte retrieval, n = 1118) and group II (denudation 2-5 h after oocyte retrieval, n = 933). Univariate analysis by two-sample t-test or Mann-Whitney test was used, as appropriate...
April 4, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29672007/how-general-semen-quality-influences-the-blastocyst-formation-rate-analysis-of-4205-ivf-cycles
#18
Mariana M Piccolomini, Tatiana Cs Bonetti, Eduardo La Motta, Paulo C Serafini, Jose R Alegretti
OBJECTIVE: To select embryos with higher implantation potential, the extended culture has been the most frequently applied strategy worldwide, and consequently leads to higher live birth rates per transfer. Sperm quality is a determining feature, and it may influence the outcomes of IVF from fertilization to embryo development. Therefore, we hypothesize that blastocyst formation may also be impaired by general semen quality. METHODS: We analyzed 4205 IVF cycles...
April 19, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29671256/effects-of-growth-hormone-on-pregnancy-rates-of-patients-with-thin-endometrium
#19
N Cui, A-M Li, Z-Y Luo, Z-M Zhao, Y-M Xu, J Zhang, A-M Yang, L-L Wang, G-M Hao, B-L Gao
PURPOSE: To investigate whether growth hormone (GH) could improve pregnancy rates of patients with thin endometrium by clinical study and laboratory experiments. MATERIALS AND METHODS: Ninety-three patients were randomized to either the GH-received group (40) or the routine exogenous administration of estrogens control group (53) for clinical study. The human endometrial carcinoma cell line RL95-2 was used for testing the role of GH with Western blot and real-time PCR by exposure to various concentrations of GH (0...
April 18, 2018: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/29669196/development-of-the-human-incus-with-special-reference-to-the-detachment-from-the-chondrocranium-to-be-transferred-into-the-middle-ear
#20
Jose Francisco Rodríguez-Vázquez, Masahito Yamamoto, Shinichi Abe, Yukio Katori, Gen Murakami
The mammalian middle ear represents one of the most fundamental features defining this class of vertebrates. However, the origin and the developmental process of the incus in the human remains controversial. The present study seeks to demonstrate all the steps of development and integration of the incus within the middle ear. We examined histological sections of 55 human embryos and foetuses at 6 to 13 weeks of development. At 6 weeks of development (16 Carnegie Stage), the incus anlage was found to be lateral to this vein at the cranial end of the first pharyngeal arch...
April 18, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
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