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Embryo transfer

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https://www.readbyqxmd.com/read/28324064/bmp7-induces-uterine-receptivity-and-blastocyst-attachment
#1
Diana Monsivais, Caterina Clementi, Jia Peng, Paul T Fullerton, Renata Prunskaite-Hyyryläinen, Seppo Vainio, Martin M Matzuk
In women, the window of implantation is limited to a brief two- to three- day period characterized by optimal levels of circulating ovarian hormones and a receptive endometrium. Although the window of implantation is assumed to occur 8-10 days after ovulation in women, molecular markers of endometrial receptivity are necessary to determine optimal timing prior to embryo transfer. Previous studies showed that members of the bone morphogenetic protein (BMP) family are expressed in the uterus necessary for female fertility; however, the role of BMP7 during implantation and in late gestation is not known...
January 17, 2017: Endocrinology
https://www.readbyqxmd.com/read/28319018/the-effect-of-elevated-progesterone-levels-before-hcg-triggering-in-modified-natural-cycle-frozen-thawed-embryo-transfer-cycles
#2
Eva R Groenewoud, Nick S Macklon, Ben J Cohlen
Recent studies suggest that elevated late follicular phase progesterone concentrations after ovarian stimulation for IVF may result in embryo-endometrial asynchrony, reducing the chance of successful implantation after fresh embryo transfer. It remains unclear to what extent elevated late follicular phase progesterone levels may occur in unstimulated cycles before frozen-thawed embryo transfer, or what affect they may have on outcomes. In this cohort study, 271 patients randomized to the modified natural cycle arm of a randomized controlled trial comparing two endometrial preparation regimens underwent late follicular phase progesterone and LH testing...
February 28, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28315625/endometrial-injury-for-women-with-previous-in-vitro-fertilization-failure-does-it-improve-pregnancy-rate
#3
(no author information available yet)
Implantation failure is one of the major factors limiting success of in vitro fertilization (IVF) treatment. According to ESHRE 2009 data only 32% of fresh embryo transfers resulted in clinical pregnancies. There are many ideas to improve the treatment outcomes, endometrial injury being one of them. It has been suggested that local endometrial injury, performed either by pipelle biopsy or hysteroscopy, may increase clinical pregnancy rate. However, up to date literature is widely disparate on that subject. There is no conclusion with regard to optimal timing, the number and technique of the procedure...
November 19, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28306341/implantation-potential-of-mosaic-embryos
#4
Belén Lledó, Ruth Morales, Jose Antonio Ortiz, Helena Blanca, Jorge Ten, Joaquín Llácer, Rafael Bernabeu
Chromosomal mosaicism is a relatively common finding in human IVF embryos. However, the association between mosaicism in trophoectoderm and inner mass cells, the mechanisms involved, and its effects on implantation are far from established. We retrospectively reanalyzed array-CGH results from 1,362 trophoectoderm biopsies. We detected chromosomal mosaicism in 183 blastocysts (13.4%). A decrease in the clinical pregnancy rate when we compared the cycles where only mosaic embryos were transferred (26.9%) vs. euploid embryos were transferred (40...
March 17, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28301674/single-cell-transcriptome-profiling-revealed-differences-in-gene-expression-during-oocyte-maturation-in-haimen-white-goats
#5
X Y Yin, G H Cheng, H Y Guo, Q Wang, Y J Li, H Zhang
Juvenile in vitro embryo transfer is an important animal reproductive technology that can shorten the generation interval of livestock, explore the reproductive potential of dams with excellent genetic traits, accelerate genetic progress and production efficiency of the herd, and provide a wealth of genetic resources for livestock breeding. However, oocytes from kids do not develop as well as those from female goats during in vitro maturation. To identify differences during different stages of oocyte maturation, we used single cell transcriptome sequencing to compare gene expression in mature oocytes from kids and female goats...
March 15, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28299549/elective-single-blastocyst-transfer-in-advanced-maternal-age
#6
Samer Tannus, Weon-Young Son, Michael Haim Dahan
PURPOSE: The purpose of this study was to investigate reproductive outcomes following elective single blastocyst transfer (eSBT) compared with those of double blastocyst transfer (DBT) in advanced maternal age. METHODS: This was a retrospective cohort study performed at an academic fertility center. All women aged 40 and over for whom in vitro fertilization (IVF) cycles were performed and in whom embryo culture was extended to the blastocyst stage were reviewed for possible inclusion...
March 15, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28292618/guidance-on-the-limits-to-the-number%C3%A2-of-embryos-to-transfer-a%C3%A2-committee-opinion
#7
(no author information available yet)
Based on American Society for Reproductive Medicine (ASRM) and Society for Assisted Reproductive Technology data available through 2014, ASRM's guidelines for the limits on the number of embryos to be transferred in in vitro fertilization (IVF) cycles have been further refined in continuing efforts to promote singleton gestation and reduce the number of multiple pregnancies. This version replaces the document titled Criteria for number of embryos to transfer: a committee opinion that was published most recently in August of 2013 (Fertil Steril 2013;99:44-6)...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28292615/perinatal-outcomes-among-singletons-after-assisted-reproductive-technology-with-single-embryo-or-double-embryo-transfer-versus-no-assisted-reproductive-technology
#8
Angela S Martin, Jeani Chang, Yujia Zhang, Jennifer F Kawwass, Sheree L Boulet, Patricia McKane, Dana Bernson, Dmitry M Kissin, Denise J Jamieson
OBJECTIVE: To examine outcomes of singleton pregnancies conceived without assisted reproductive technology (non-ART) compared with singletons conceived with ART by elective single-embryo transfer (eSET), nonelective single-embryo transfer (non-eSET), and double-embryo transfer with the establishment of 1 (DET -1) or ≥2 (DET ≥2) early fetal heartbeats. DESIGN: Retrospective cohort using linked ART surveillance data and vital records from Florida, Massachusetts, Michigan, and Connecticut...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28292614/embryo-transfer-simulation-improves-pregnancy-rates-and-decreases-time-to-proficiency-in-reproductive-endocrinology-and-infertility-fellow-embryo-transfers
#9
Ryan J Heitmann, Micah J Hill, John M Csokmay, Justin Pilgrim, Alan H DeCherney, Shad Deering
OBJECTIVE: To design and evaluate an ET simulator to train Reproductive Endocrinology and Infertility (REI) fellows' techniques of ET. DESIGN: Simulation model development and retrospective cohort analysis. SETTING: Not applicable. PATIENT(S): Patients undergoing IVF. INTERVENTION(S): Simulation model evaluation and implementation of ET simulation training. MAIN OUTCOME MEASURE(S): Pregnancy rates...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28292612/perinatal-outcomes-in-children-born-after-fresh-or-frozen-embryo-transfer-a-catalan-cohort-study-based-on-14-262-newborns
#10
Mar Vidal, Kilian Vellvé, Mireia González-Comadran, Ana Robles, Maria Prat, Mar Torné, Ramón Carreras, Miguel A Checa
OBJECTIVE: To ascertain whether perinatal outcomes are affected by vitrification and/or controlled ovarian hyperstimulation (COH). DESIGN: Register-based cohort study. SETTING: Not applicable. PATIENT(S): Women undergoing in vitro fertilization (IVF) between 2008 and 2012 using autologous or donated eggs who had a singleton pregnancy delivered from the 24th week onward. INTERVENTION(S): Fresh embryo transfer (ET) or frozen-thawed ET in women undergoing IVF...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28292611/asrm-standard-embryo-transfer-protocol-template-a-committee-opinion
#11
Alan Penzias, Kristin Bendikson, Samantha Butts, Christos Coutifaris, Tommaso Falcone, Gregory Fossum, Susan Gitlin, Clarisa Gracia, Karl Hansen, Jennifer Mersereau, Randall Odem, Robert Rebar, Richard Reindollar, Mitchell Rosen, Jay Sandlow, Michael Vernon
Standardization improves performance and safety. A template for standardizing the embryo transfer procedure is presented here with 12 basic steps supported by published scientific literature and a survey of common practice of SART programs; it can be used by ART practices to model their own standard protocol.
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28291830/soluble-cd146-an-innovative-and-non-invasive-biomarker-of-embryo-selection-for-in-vitro-fertilization
#12
Sylvie Bouvier, Odile Paulmyer-Lacroix, Nicolas Molinari, Alexandrine Bertaud, Marine Paci, Aurélie Leroyer, Stéphane Robert, Françoise Dignat George, Marcel Blot-Chabaud, Nathalie Bardin
Although progress was made in in vitro fertilization (IVF) techniques, the majority of embryos transferred fail to implant. Morphology embryo scoring is the standard procedure for most of IVF centres for choosing the best embryo, but remains limited since even the embryos classified as "top quality" may not implant. As it has been shown that i) CD146 is involved in embryo implantation and ii) membrane form is shed to generate soluble CD146 (sCD146), we propose that sCD146 in embryo supernatants may constitute a new biomarker of embryo selection...
2017: PloS One
https://www.readbyqxmd.com/read/28288882/clinical-outcomes-of-patients-with-heterotopic-pregnancy-after-surgical-treatment
#13
Yiqi Guan, Caihong Ma
STUDY OBJECTIVE: This study aimed to review surgical management and clinical outcomes of patients with heterotopic pregnancy (HP) who underwent in vitro fertilization and embryo transfer (IVF-ET) and surgical treatment at the Assisted Reproductive Technology Center of Peking University Third Hospital from January 2010 to December 2015. DESIGN: We retrospectively analyzed 56 patients' general characteristics, diagnostic features, surgical management, and clinical outcomes according to medical records and follow-up telephone interviews...
March 10, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28288197/dog-cloning-with-in-vivo-matured-oocytes-obtained-using-electric-chemiluminescence-immunoassay-predicted-ovulation-method
#14
Seunghoon Lee, Minghui Zhao, Jingu No, Yoonseok Nam, Gi-Sun Im, Tai-Young Hur
Radioactive immunoassay (RIA) is a traditional serum hormone assay method, but the application of the method in reproductive studies is limited by the associated radioactivity. The aim of present study was to evaluate the reliability of RIA and to compare its canine serum progesterone concentration determination accuracy to that of the electric chemiluminescence immunoassay (ECLI). In vivo matured oocytes were utilized for canine somatic cell nuclear transfer (SCNT), and serum progesterone levels were assessed to accurately determine ovulation and oocyte maturation...
2017: PloS One
https://www.readbyqxmd.com/read/28285882/effect-of-altering-the-intervals-between-consecutive-superovulatory-doses-of-porcine-follicle-stimulating-hormone-on-ovarian-responses-and-embryo-yields-in-anestrous-ewes
#15
P M Bartlewski, M Murawski, T Schwarz, M E F Oliveira
The effect of varying intervals between successive gonadotropin injections on the superovulatory outcomes in anestrous Rideau Arcott ewes superstimulated for ovarian follicular development with multiple doses of porcine FSH (pFSH) was evaluated in a single study. Twenty-five animals received six (1×2.5ml and 5×1.25ml) injections of Folltropin(®)-V given at 0800 and 1600h or at 0800 and 2000h in Group 1 (n=9) or Group 2 (n=16), respectively. An i.m. injection of 500 IU of equine chorionic gonadotropin (eCG; Folligon(®)) was given concurrently with the first pFSH dose...
March 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28285056/unilateral-salpingectomy-and-methotrexate-are-associated-with-similar-recurrence-rate-of-ectopic-pregnancy-in-patients-undergoing-ivf
#16
Mohamad Irani, Alex Robles, Vinay Gunnala, Steven D Spandorfer
STUDY OBJECTIVE: To determine whether different treatment approaches of ectopic pregnancy (EP), particularly unilateral salpingectomy and methotrexate, affect its recurrence rate in patients undergoing in vitro fertilization (IVF). DESIGN: Retrospective cohort study. DESIGN: Classification: Canadian Task Force Classification II-2. SETTING: Academic medical center. PATIENTS: Patients with a history of previous EP who achieved pregnancy following IVF cycles between January 2004 and August 2015 were included...
March 8, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28281144/predictive-value-of-spindle-retardance-in-embryo-implantation-rate
#17
Sabela García-Oro, María Isabel Rey, Marta Rodríguez, Ángel Durán, Roque Devesa, Diana Valverde
PURPOSE: We evaluated the relationship between meiotic spindle characteristics and in vitro fertilization cycle outcome. METHODS: Five hundred sixty-nine oocytes from 86 in vitro fertilization cycles were analyzed for fertilization and subsequent implantation rates. Oocytes were assessed for maturation status. The oocytes and embryos were cultured in sequential and nonsequential media (G Series, Vitrolife, Sweden) and incubated in 6% CO2, 5% O2 at 37 °C. Two hours following oocyte decumulation (38-39 h post-hCG/GnRH administration) and prior to microinjection, the structure of the meiotic spindle was assessed using the Oosight Imaging System (CRI, UK)...
March 9, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28277886/can-we-alter-pregnancy-outcome-by-adjusting-progesterone-treatment-at-mid-luteal-phase-a-randomized-controlled-trial
#18
Nardin Aslih, Adrian Ellenbogen, Tal Shavit, Medeia Michaeli, Devora Yakobi, Einat Shalom-Paz
Our study aimed to determine whether mid-luteal serum P concentrations can serve as a predictive factor for in vitro fertilization (IVF) outcomes and whether increasing P dosage for patients with low levels at mid-luteal phase may improve pregnancy rates. It was a prospective, randomized controlled study. A total of 146 patients undergoing IVF treatment were prospectively enrolled and received routine luteal phase support (LPS) regimen of Endometrin® (progesterone) 200 mg/day. Serum P levels were measured 7 days after embryo transfer (ET)...
March 9, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28277611/e-subgroup-ppr-protein-defective-kernel-36-is-required-for-multiple-mitochondrial-transcripts-editing-and-seed-development-in-maize-and-arabidopsis
#19
Gang Wang, Mingyu Zhong, Bilian Shuai, Jiandong Song, Jie Zhang, Liang Han, Huiling Ling, Yuanping Tang, Guifeng Wang, Rentao Song
Mitochondria are semi-autonomous organelles that are the powerhouse of the cells. Plant mitochondrial RNA editing guided by pentatricopeptide repeat (PPR) proteins is essential for energy production. We identify a maize defective kernel mutant dek36, which produces small and collapsed kernels, leading to embryos and/or seedlings lethality. Seed filling in dek36 is drastically impaired, in line with the defects observed in the organization of endosperm transfer tissue. Positional cloning reveals that DEK36, encoding a mitochondria-targeted E+ subgroup PPR protein, is required for mitochondrial RNA editing at atp4-59, nad7-383 and ccmFN -302, thus resulting in decreased activities of mitochondrial complex I, complex III and complex IV in dek36...
March 9, 2017: New Phytologist
https://www.readbyqxmd.com/read/28277137/the-decline-in-serum-estradiol-on-the-second-day-after-oocyte-retrieval-affects-the-outcome-of-ivf-icsi-et-treatment-in-high-ovarian-responders
#20
Xueyan Bai, Yang Zhang, Shan Liu, Danni Qu, Hui Su, Haiying Ren, Yuan Li
OBJECTIVE: To investigate the predictive value of the decline in serum estradiol on the second day after oocyte retrieval on the outcomes of in vitro fertilization (IVF) or intra-cytoplasmic sperm injection and embryo transfer (ICSI-ET) among high ovarian responders. DESIGN: Retrospective single-center cohort study. SETTING: Tertiary-care, university-affiliated teaching hospital. Patients Women aged 20-45 years undergoing assisted reproduction treatment from June 2014 to December 2015...
February 21, 2017: Gynecological Endocrinology
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