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https://www.readbyqxmd.com/read/27914926/metschnikowia-cf-typographi-and-other-pathogens-from-the-bark-beetle-ips-sexdentatus-prevalence-histological-and-ultrastructural-evidence-and-molecular-characterization
#1
Regina G Kleespies, Young Woon Lim, Cezary Tkaczuk, Marta Wrzosek, Bernhardt M Steinwender, Rudolf Wegensteiner
Ips sexdentatus (Six-spined engraver beetle) from Austria and Poland were dissected and examined for the presence of pathogens. Specimens collected in Austria were found to contain the ascomycetous fungus Metschnikowia cf. typographi. Infection rates ranged from 3.6% to 26.8% at different collection sites. M. cf. typographi infected midguts were investigated by histological, ultrastructural and molecular techniques. Extraordinary ultrastructural details are shown, such as ascospores with bilateral flattened flanks resembling alar rims at both sides of their attenuating tube-like ends...
November 30, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27889478/sequential-enzymatic-saccharification-and-fermentation-of-ionic-liquid-and-organosolv-pretreated-agave-bagasse-for-ethanol-production
#2
Jose A Pérez-Pimienta, Alejandra Vargas-Tah, Karla M López-Ortega, Yessenia N Medina-López, Jorge A Mendoza-Pérez, Sayeny Avila, Seema Singh, Blake A Simmons, Inés Loaces, Alfredo Martinez
Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass...
November 16, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27869799/transcript-protein-and-metabolite-temporal-dynamics-in-the-cam-plant-agave
#3
Paul E Abraham, Hengfu Yin, Anne M Borland, Deborah Weighill, Sung Don Lim, Henrique Cestari De Paoli, Nancy Engle, Piet C Jones, Ryan Agh, David J Weston, Stan D Wullschleger, Timothy Tschaplinski, Daniel Jacobson, John C Cushman, Robert L Hettich, Gerald A Tuskan, Xiaohan Yang
Already a proven mechanism for drought resilience, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency by means of an inverse (compared to C3 and C4 photosynthesis) day/night pattern of stomatal closure/opening to shift CO2 uptake to the night, when evapotranspiration rates are low. A systems-level understanding of temporal molecular and metabolic controls is needed to define the cellular behaviour underpinning CAM. Here, we report high-resolution temporal behaviours of transcript, protein and metabolite abundances across a CAM diel cycle and, where applicable, compare the observations to the well-established C3 model plant Arabidopsis...
November 21, 2016: Nature Plants
https://www.readbyqxmd.com/read/27853904/overexpression-of-smorf-ynr034w-a-ego4-in-saccharomyces-cerevisiae-increases-the-fermentative-efficiency-of-agave-tequilana-weber-must
#4
Naurú Idalia Vargas-Maya, Gloria Angélica González-Hernández, Israel Enrique Padilla-Guerrero, Juan Carlos Torres-Guzmán
Fermentative processes are widely used to produce food, beverages and biofuels. Saccharomyces cerevisiae is an efficient ethanol-producing microorganism. However, a concentration of high ethanol and other metabolites can affect yeast viability and decrease the ethanol yield. Many studies have focused on improving the fermentative efficiency, mostly through the genetic engineering of genes that have a direct impact on specific metabolic pathways. In the present study, we characterized a small open reading frame encoding a protein with an unknown function and biological role termed YNR034W-A...
November 16, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27792720/-effect-of-simvastatin-plus-inulin-in-comparison-with-simvastatin-plus-ezetimibe-on-the-treatment-of-mixed-dyslipidemia
#5
Esperanza Martínez-Abundis, Carmelita Barrera-Durán, Manuel González-Ortiz, Eduardo Hernández-Salazar
INTRODUCTION: Mixed dyslipidemia accelerates atherosclerosis and leads to cardiovascular disease and death. Non-soluble fibers such as inulin have been shown to have an effect on dyslipidemia. AIM: To assess the effect of the combination of simvastatin plus inulin in comparison with simvastatin plus ezetimibe in mixed dyslipidemia. MATERIAL AND METHODS: A randomized, double-blind, clinical trial with parallel control group was performed in 60 patients with mixed dyslipidemia, without drug treatment or failure to statins and lifestyle changes...
October 2016: Gaceta Médica de México
https://www.readbyqxmd.com/read/27786154/spectroscopic-and-microscopic-study-of-peroxyformic-pulping-of-agave-waste
#6
Hilda M Hernández-Hernández, Jorge J Chanona-Pérez, Alberto Vega, Pablo Ligero, Reynold R Farrera-Rebollo, Jorge A Mendoza-Pérez, Georgina Calderón-Domínguez, Norma Güemes Vera
The peroxyformic process is based on the action of a carboxylic acid (mainly formic acid) and the corresponding peroxyacid. The influences of processing time (60-180 min), formic acid concentration (80-95%), temperature (60-80°C), and hydrogen peroxide concentration (2-4%) on peroxyformic pulping of agave leaves were studied by surface response methodology using a face-centered factorial design. Empirical models were obtained for the prediction of yield, κ number (KN) and pulp viscosity as functions of the aforementioned variables...
October 2016: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/27777945/the-effects-of-agave-fourcroydes-powder-as-a-dietary-supplement-on-growth-performance-gut-morphology-concentration-of-igg-and-hematology-parameters-in-broiler-rabbits
#7
Maidelys Iser, Yordan Martínez, Hengjia Ni, Hongmei Jiang, Manuel Valdivié Navarro, Xiaosong Wu, Naif Abdullah Al-Dhabi, Manuel Rosales, Veeramuthu Duraipandiyan, Jun Fang
This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27730409/fructan-active-enzymes-fazy-activities-and-biosynthesis-of-fructooligosaccharides-in-the-vacuoles-of-agave-tequilana-weber-blue-variety-plants-of-different-age
#8
Erika Mellado-Mojica, Luis E González de la Vara, Mercedes G López
Biosynthesis of agave fructans occurs in mesontle vacuoles which showed fluctuations in FAZY activities and synthesized a diverse spectrum of fructooligosaccharide isomers. Agave tequilana Weber Blue variety is an important agronomic crop in Mexico. Fructan metabolism in A. tequilana exhibits changes in fructan content, type, degree of polymerization (DP), and molecular structure. Specific activities of vacuolar fructan active enzymes (FAZY) in A. tequilana plants of different age and the biosynthesis of fructooligosaccharides (FOSs) were analyzed in this work...
October 11, 2016: Planta
https://www.readbyqxmd.com/read/27679439/fructans-from-agave-tequilana-with-a-lower-degree-of-polymerization-prevent-weight-gain-hyperglycemia-and-liver-steatosis-in-high-fat-diet-induced-obese-mice
#9
A L Márquez-Aguirre, R M Camacho-Ruíz, Y K Gutiérrez-Mercado, E Padilla-Camberos, M González-Ávila, F J Gálvez-Gastélum, N E Díaz-Martínez, D Ortuño-Sahagún
Fructans from agave have received specific attention because of their highly branched fructan content. We have previously reported that the degree of polymerization (dp) influences their biological activity. Therefore, the aim of this study was to investigate the effect of unfractionated and fractionated fructans (higher and lower dps) from Agave tequilana in high-fat diet-induced (HFD) obese mice. Fructans with a lower dp (HFD+ScF) decreased weight gain by 30 %, body fat mass by 51 %, hyperglycemia by 25 % and liver steatosis by 40 %...
December 2016: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/27678062/aguamiel-concentrate-from-agave-salmiana-and-its-extracted-saponins-attenuated-obesity-and-hepatic-steatosis-and-increased-akkermansia-muciniphila-in-c57bl6-mice
#10
Ana María Leal-Díaz, Lilia G Noriega, Ivan Torre-Villalvazo, Nimbe Torres, Gabriela Alemán-Escondrillas, Patricia López-Romero, Mónica Sánchez-Tapia, Miriam Aguilar-López, Janette Furuzawa-Carballeda, Laura A Velázquez-Villegas, Azalia Avila-Nava, Guillermo Ordáz, Janet A Gutiérrez-Uribe, Sergio O Serna-Saldivar, Armando R Tovar
Obesity and its comorbidities are a severe public health problem worldwide. The use of bioactive compounds found in some foods has been demonstrated to ameliorate the metabolic abnormalities associated with obesity. The purpose of this study was to assess whether the bioactive compounds present in aguamiel concentrate (AC) from Agave salmiana could attenuate glucose intolerance and hepatic steatosis in mice fed a high fat (HF) diet. HPLC-ELSD analysis showed that AC contained several saponins. The consumption of an AC extract rich in saponins reduced weight gain and fat mass and lowered serum glucose, insulin and LDL-cholesterol levels in mice fed a HF diet...
September 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27676375/green-synthesis-characterisation-and-biological-evaluation-of-agnps-using-agave-americana-mentha-spicata-and-mangifera-indica-aqueous-leaves-extract
#11
Bashir Ahmad, Farah Shireen, Shumaila Bashir, Ibrar Khan, Sadiq Azam
The current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (AgNPs) of Agave americana, Mentha spicata and Mangifera indica leaves and to screen them for biological activities. The ultraviolet-visible spectroscopic analysis revealed that λ-max for AgNPs range from 350-500 nm. All AgNPs possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in X-ray diffraction measurements and supported by scanning electron microscopy data...
October 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27660789/draft-genome-sequence-of-heavy-metal-resistant-cupriavidus-alkaliphilus-asc-732t-isolated-from-agave-rhizosphere-in-the-northeast-of-mexico
#12
Fernando Uriel Rojas-Rojas, Marcel Huntemann, Alicia Clum, Manoj Pillay, Krishnaveni Palaniappan, Neha Varghese, Natalia Mikhailova, Dimitrios Stamatis, T B K Reddy, Victor Markowitz, Natalia Ivanova, Nikos Kyrpides, Tanja Woyke, Nicole Shapiro, J Antonio Ibarra, Paulina Estrada-de Los Santos
Cupriavidus alkaliphilus ASC-732(T) was isolated from the rhizosphere of agave plant growing in alkaline soils in San Carlos, Tamaulipas, Mexico. The species is able to grow in the presence of arsenic, zinc, and copper. The genome sequence of strain ASC-732(T) is 6,125,055 bp with 5,586 genes and an average G+C content of 67.81%.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27652180/methanol-contamination-in-traditionally-fermented-alcoholic-beverages-the-microbial-dimension
#13
REVIEW
Elijah Ige Ohimain
Incidence of methanol contamination of traditionally fermented beverages is increasing globally resulting in the death of several persons. The source of methanol contamination has not been clearly established in most countries. While there were speculations that unscrupulous vendors might have deliberately spiked the beverages with methanol, it is more likely that the methanol might have been produced by contaminating microbes during traditional ethanol fermentation, which is often inoculated spontaneously by mixed microbes, with a potential to produce mixed alcohols...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27612732/synthesis-of-silver-nanoparticles-using-aqueous-extracts-of-heterotheca-inuloides-as-reducing-agent-and-natural-fibers-as-templates-agave-lechuguilla-and-silk
#14
Raúl A Morales-Luckie, Aldo Adrián Lopezfuentes-Ruiz, Oscar F Olea-Mejía, Argueta-Figueroa Liliana, Víctor Sanchez-Mendieta, Witold Brostow, Juan P Hinestroza
Silver nanoparticles (Ag NPs) were synthesized using a one-pot green methodology with aqueous extract of Heterotheca inuloides as a reducing agent, and the support of natural fibers: Agave lechuguilla and silk. UV-Vis spectroscopy, X-Ray photoelectron spectroscopy XPS and transmission electron microscopy TEM were used to characterize the resulting bionanocomposite fibers. The average size of the Ag NPs was 16nm and they exhibited low polydispersity. XPS studies revealed the presence of only metallic Ag in the nanoparticles embedded in Agave...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27590059/physiological-differences-and-changes-in-global-dna-methylation-levels-in-agave-angustifolia-haw-albino-variant-somaclones-during-the-micropropagation-process
#15
Fátima Duarte-Aké, Eduardo Castillo-Castro, Felipe Barredo Pool, Francisco Espadas, Jorge M Santamaría, Manuel L Robert, Clelia De-la-Peña
Global DNA methylation changes caused by in vitro conditions are associated with the subculturing and phenotypic variation in Agave angustifolia Haw. While the relationship between the development of albinism and in vitro culture is well documented, the role of epigenetic processes in this development leaves some important questions unanswered. During the micropropagation of Agave angustifolia Haw., we found three different phenotypes, green (G), variegated (V) and albino (A). To understand the physiological and epigenetic differences among the somaclones, we analyzed several morphophysiological parameters and changes in the DNA methylation patterns in the three phenotypes during their in vitro development...
September 2, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27573548/use-of-agave-tequilana-lignin-and-zinc-oxide-nanoparticles-for-skin-photoprotection
#16
José Manuel Gutiérrez-Hernández, Alfredo Escalante, Raquel Nalleli Murillo-Vázquez, Ezequiel Delgado, Francisco Javier González, Guillermo Toríz
The use of sunscreens is essential for preventing skin damage and the potential appearance of skin cancer in humans. Inorganic active components such as zinc oxide (ZnO) have been used commonly in sunscreens due to their ability to block UVA radiation. This ultraviolet (UV) protection might be enhanced to cover the UVB and UVC bands when combined with other components such as titanium dioxide (TiO2). In this work we evaluate the photoprotection properties of organic nanoparticles made from lignin in combination with ZnO nanoparticles as active ingredients for sunscreens...
October 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27534411/cellulases-immobilization-on-chitosan-coated-magnetic-nanoparticles-application-for-agave-atrovirens-lignocellulosic-biomass-hydrolysis
#17
Jaquelina Sánchez-Ramírez, José L Martínez-Hernández, Patricia Segura-Ceniceros, Guillermo López, Hened Saade, Miguel A Medina-Morales, Rodolfo Ramos-González, Cristóbal N Aguilar, Anna Ilyina
In the present study, Trichoderma reesei cellulase was covalently immobilized on chitosan-coated magnetic nanoparticles using glutaraldehyde as a coupling agent. The average diameter of magnetic nanoparticles before and after enzyme immobilization was about 8 and 10 nm, respectively. The immobilized enzyme retained about 37 % of its initial activity, and also showed better thermal and storage stability than free enzyme. Immobilized cellulase retained about 80 % of its activity after 15 cycles of carboxymethylcellulose hydrolysis and was easily separated with the application of an external magnetic field...
August 17, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27490526/agavins-increase-neurotrophic-factors-and-decrease-oxidative-stress-in-the-brains-of-high-fat-diet-induced-obese-mice
#18
Elena Franco-Robles, Mercedes G López
BACKGROUND: Fructans obtained from agave, called agavins, have recently shown significant benefits for human health including obesity. Therefore, we evaluated the potential of agavins as neuroprotectors and antioxidants by determining their effect on brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF) as well as oxidative brain damage in of obese mice. METHODS: Male C57BL/6J mice were fed a high-fat diet (HFD) and treated daily with 5% (HFD/A5) or 10% (HFD/A10) of agavins or a standard diet (SD) for 10 weeks...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27447701/improvement-on-the-productivity-of-continuous-tequila-fermentation-by-saccharomyces-cerevisiae-of-agave-tequilana-juice-with-supplementation-of-yeast-extract-and-aeration
#19
Guillermo Hernández-Cortés, Juan Octavio Valle-Rodríguez, Enrique J Herrera-López, Dulce María Díaz-Montaño, Yolanda González-García, Héctor B Escalona-Buendía, Jesús Córdova
Agave (Agave tequilana Weber var. azul) fermentations are traditionally carried out employing batch systems in the process of tequila manufacturing; nevertheless, continuous cultures could be an attractive technological alternative to increase productivity and efficiency of sugar to ethanol conversion. However, agave juice (used as a culture medium) has nutritional deficiencies that limit the implementation of yeast continuous fermentations, resulting in high residual sugars and low fermentative rates. In this work, fermentations of agave juice using Saccharomyces cerevisiae were put into operation to prove the necessity of supplementing yeast extract, in order to alleviate nutritional deficiencies of agave juice...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27446061/pulque-a-traditional-mexican-alcoholic-fermented-beverage-historical-microbiological-and-technical-aspects
#20
REVIEW
Adelfo Escalante, David R López Soto, Judith E Velázquez Gutiérrez, Martha Giles-Gómez, Francisco Bolívar, Agustín López-Munguía
Pulque is a traditional Mexican alcoholic beverage produced from the fermentation of the fresh sap known as aguamiel (mead) extracted from several species of Agave (maguey) plants that grow in the Central Mexico plateau. Currently, pulque is produced, sold and consumed in popular districts of Mexico City and rural areas. The fermented product is a milky white, viscous, and slightly acidic liquid beverage with an alcohol content between 4 and 7° GL and history of consumption that dates back to pre-Hispanic times...
2016: Frontiers in Microbiology
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