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https://www.readbyqxmd.com/read/28546437/characterization-of-thermostable-cam-rubisco-activase-reveals-a-rubisco-interacting-surface-loop
#1
Devendra Shivhare, Oliver Mueller-Cajar
In order to maintain metabolic flux through the Calvin-Benson-Bassham (CBB) cycle in higher plants, dead-end inhibited complexes of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) must constantly be engaged and remodeled by the molecular chaperone Rubisco activase (Rca). In C3 plants the thermolability of Rca is responsible for the deactivation of Rubisco and reduction of photosynthesis at moderately elevated temperatures. We reasoned that CAM plants must possess thermostable Rca to support CBB flux during the day when stomata are closed...
May 25, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28497409/a-rapid-and-reliable-method-for-total-protein-extraction-from-succulent-plants-for-proteomic-analysis
#2
Fernando Lledías, Felipe Hernández, Viridiana Rivas, Abisaí García-Mendoza, Gladys I Cassab, Jorge Nieto-Sotelo
Crassulacean acid metabolism plants have some morphological features, such as succulent and reduced leaves, thick cuticles, and sunken stomata that help them prevent excessive water loss and irradiation. As molecular constituents of these morphological adaptations to xeric environments, succulent plants produce a set of specific compounds such as complex polysaccharides, pigments, waxes, and terpenoids, to name a few, in addition to uncharacterized proteases. Since all these compounds interfere with the analysis of proteins by electrophoretic techniques, preparation of high quality samples from these sources represents a real challenge...
May 12, 2017: Protein Journal
https://www.readbyqxmd.com/read/28444599/study-of-enzymatic-saccharification-of-agave-leaves-biomass-to-yield-fermentable-sugars
#3
Miguel A Medina-Morales, Oscar Soto-Cruz, Juan C Contreras-Esquivel, Raúl Rodríguez-Herrera, Heliodoro De la Garza-Toledo, Cristóbal N Aguilar
Agave is a good source of polysaccharides for the production of fermentable sugars as sustainable bioenergy feedstock solutions for semi-arid and arid lands. This plant grows in arid areas, which correspond to a large territory in northern Mexico. Having lignocellulose as the polysaccharide of interest, the information for the enzymatic saccharification of this kind of material is limited. Agave cell walls have a unique recalcitrant nature, but having a high cellulose content, makes this plant material an interesting research subject...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28441627/effect-of-in%C3%A2-vitro-drought-stress-on-phenolic-acids-flavonols-saponins-and-antioxidant-activity-in-agave-salmiana
#4
César A Puente-Garza, Cristina Meza-Miranda, Desiree Ochoa-Martínez, Silverio García-Lara
This work evaluated the effect of in vitro drought stress on morphological characteristics, phenolic compounds, flavonols, saponin content, and antioxidant activity in plantlets of Agave salmiana. Drought stress was induced with polyethylene glycol (PEG) at 0, 10, 20 and 30% w/v in Murashige and Skoog solid medium. The determination of specific flavonols and saponins was achieved via HPLC-DAD and HPLC-ELSD, respectively. Compared with the control, plants grown in 30% PEG showed a change in the width of the leaves and a different color, showing less clarity and more darkening (L = 21...
April 10, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28344647/ternary-ionic-liquid-water-pretreatment-systems-of-an-agave-bagasse-and-municipal-solid-waste-blend
#5
Jose A Perez-Pimienta, Noppadon Sathitsuksanoh, Vicki S Thompson, Kim Tran, Teresa Ponce-Noyola, Vitalie Stavila, Seema Singh, Blake A Simmons
BACKGROUND: Pretreatment is necessary to reduce biomass recalcitrance and enhance the efficiency of enzymatic saccharification for biofuel production. Ionic liquid (IL) pretreatment has gained a significant interest as a pretreatment process that can reduce cellulose crystallinity and remove lignin, key factors that govern enzyme accessibility. There are several challenges that need to be addressed for IL pretreatment to become viable for commercialization, including IL cost and recyclability...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28341907/utilization-of-inulin-containing-waste-in-industrial-fermentations-to-produce-biofuels-and-bio-based-chemicals
#6
REVIEW
Stephen R Hughes, Nasib Qureshi, Juan Carlos López-Núñez, Marjorie A Jones, Joshua M Jarodsky, Luz Ángela Galindo-Leva, Mitchell R Lindquist
Inulins are polysaccharides that belong to an important class of carbohydrates known as fructans and are used by many plants as a means of storing energy. Inulins contain 20 to several thousand fructose units joined by β-2,1 glycosidic bonds, typically with a terminal glucose unit. Plants with high concentrations of inulin include: agave, asparagus, coffee, chicory, dahlia, dandelion, garlic, globe artichoke, Jerusalem artichoke, jicama, onion, wild yam, and yacón. To utilize inulin as its carbon and energy source directly, a microorganism requires an extracellular inulinase to hydrolyze the glycosidic bonds to release fermentable monosaccharides...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28330185/agave-atrovirens-fibers-as-substrate-and-support-for-solid-state-fermentation-for-cellulase-production-by-trichoderma-asperellum
#7
Naivy Y Nava-Cruz, Juan C Contreras-Esquivel, Miguel A Aguilar-González, Alberto Nuncio, Raúl Rodríguez-Herrera, Cristóbal N Aguilar
Many efforts have been made to produce cellulase with better features and conditions, and filamentous fungi have played an important role in the bioprocess, growing in liquid and solid cultures with sugarcane bagasse, corn stover and others lignocellulosic materials. In the present study, Agave atrovirens fibers were partially characterized, thermal pretreated and used as support, substrate and inducer source for cellulolytic complex production by four strains of the genus Trichoderma, where T. asperellum was selected as the best option for this process after evaluating the enzyme activity and the invasion capacity on the pretreated Agave fibers...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28325555/autohydrolysis-pretreatment-assessment-in-ethanol-production-from-agave-bagasse
#8
Leopoldo J Rios-González, Thelma K Morales-Martínez, María F Rodríguez-Flores, José A Rodríguez-De la Garza, David Castillo-Quiroz, Agustín J Castro-Montoya, Alfredo Martinez
The aim of the present work was to assess the autohydrolysis pretreatment of Agave tequilana bagasse for ethanol production. The pretreatment was conducted using a one-liter high pressure Parr reactor under different severity factors (SF) at a 1:6w/v ratio (solid:liquid) and 200rpm. The solids obtained under the selected autohydrolysis conditions were subjected to enzymatic hydrolysis with a commercial cellulase cocktail, and the enzymatic hydrolysate was fermented using Saccharomyces cerevisiae. The results obtained from the pretreatment process showed that the glucan content in the pretreated solid was mostly preserved, and an increase in the digestibility was observed for the case with a SF of 4...
March 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28325323/in-depth-study-of-agave-fiber-structure-using-fourier-transform-infrared-spectroscopy
#9
Asma El Oudiani, Slah Msahli, Faouzi Sakli
FTIR spectroscopy is a powerful method to analyse materials and especially fiber structure. This technique is largely used to obtain quick information on the fiber composition and to detect possible transformations after physical and chemical treatments. Unlike other techniques, FTIR is a quick method which demands a reduced amount of fibers. Furthermore, it is a non destructive method especially for Attenuated Total Reflectance (ATR), and Near Infrared (NIR) spectroscopy which are nowadays widely used. In this work, FTIR spectrum of agave fiber has been thoroughly investigated...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28293641/functional-effects-of-prebiotic-fructans-in-colon-cancer-and-calcium-metabolism-in-animal-models
#10
Marisol Rivera-Huerta, Vania Lorena Lizárraga-Grimes, Ibrahim Guillermo Castro-Torres, Mabel Tinoco-Méndez, Lucía Macías-Rosales, Francisco Sánchez-Bartéz, Graciela Guadalupe Tapia-Pérez, Laura Romero-Romero, María Isabel Gracia-Mora
Inulin-type fructans are polymers of fructose molecules and are known for their capacity to enhance absorption of calcium and magnesium, to modulate gut microbiota and energy metabolism, and to improve glycemia. We evaluated and compared the effects of Chicory inulin "Synergy 1®" and inulin from Mexican agave "Metlin®" in two experimental models of colon cancer and bone calcium metabolism in mice and rats. Inulins inhibited the development of dextran sulfate sodium-induced colitis and colon cancer in mice; these fructans reduced the concentration of tumor necrosis factor alpha and prevented the formation of intestinal polyps, villous atrophy, and lymphoid hyperplasia...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28106207/natural-sweetener-agave-inhibits-gastric-emptying-in-rats-by-a-cholecystokinin-2-and-glucagon-like-peptide-1-receptor-dependent-mechanism
#11
E Bihter Gürler, Dilek Özbeyli, Hülya Buzcu, Sezin Bayraktar, İrem Carus, Beyza Dağ, Yasemin Geriş, Seda Jeral, Berrak Ç Yeğen
Low-calorie sweeteners are considered to be beneficial in calorie control, but the impact of these sweeteners on gastric emptying is not well described. The purpose of this study was to compare the gastric emptying rate of agave nectar with those of glucose and fructose, and to evaluate the interaction of cholecystokinin (CCK)-1, CCK-2 and glucagon-like peptide-1 (GLP-1) receptors in agave-induced alterations in gastric emptying. Female Sprague-Dawley rats were fitted with gastric cannulas. Following the recovery, the gastric emptying rates of glucose, fructose and agave at 12...
February 22, 2017: Food & Function
https://www.readbyqxmd.com/read/28005446/the-effect-of-agave-tequilana-weber-inulin-on-postprandial-ghrelin-concentration-in-obese-patients
#12
RANDOMIZED CONTROLLED TRIAL
Betsabe Contreras-Haro, Jose A Robles-Cervantes, Manuel Gonzalez-Ortiz, Esperanza Martinez-Abundis, Claudia Espinel-Bermudez, Martha P Gallegos-Arreola, Karina C Morgado-Castillo
This study was performed to investigate the effect of Agave tequilana Weber inulin on postprandial ghrelin levels in obese patients. A randomized, double-blind, cross-over design was performed. A total of 14 patients were allocated into two groups: one group received a drink that contained 500 mL lemon water, 24 g of A. tequilana Weber inulin, and 75 g glucose and the other group received a placebo drink with 500 mL lemon drink and 75 g of glucose. After a 7-day washout period, the groups were crossed...
February 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/27999975/erratum-to-physiological-differences-and-changes-in-global-dna-methylation-levels-in-agave-angustifolia-haw-albino-variant-somaclones-during-the-micropropagation-process
#13
Fátima Duarte-Aké, Eduardo Castillo-Castro, Felipe Barredo Pool, Francisco Espadas, Jorge M Santamaría, Manuel L Robert, Clelia De-la-Peña
No abstract text is available yet for this article.
December 20, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27914926/metschnikowia-cf-typographi-and-other-pathogens-from-the-bark-beetle-ips-sexdentatus-prevalence-histological-and-ultrastructural-evidence-and-molecular-characterization
#14
Regina G Kleespies, Young Woon Lim, Cezary Tkaczuk, Marta Wrzosek, Bernhardt M Steinwender, Rudolf Wegensteiner
Ips sexdentatus (six-spined engraver beetle) from Austria and Poland were dissected and examined for the presence of pathogens. Specimens collected in Austria were found to contain the ascomycetous fungus Metschnikowia cf. typographi. Infection rates ranged from 3.6% to 26.8% at different collection sites. M. cf. typographi infected midguts were investigated by histological, ultrastructural and molecular techniques. Extraordinary ultrastructural details are shown, such as ascospores with bilateral flattened flanks resembling alar rims at both sides of their attenuating tube-like ends...
December 1, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27889478/sequential-enzymatic-saccharification-and-fermentation-of-ionic-liquid-and-organosolv-pretreated-agave-bagasse-for-ethanol-production
#15
Jose A Pérez-Pimienta, Alejandra Vargas-Tah, Karla M López-Ortega, Yessenia N Medina-López, Jorge A Mendoza-Pérez, Sayeny Avila, Seema Singh, Blake A Simmons, Inés Loaces, Alfredo Martinez
Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass...
February 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27869799/transcript-protein-and-metabolite-temporal-dynamics-in-the-cam-plant-agave
#16
Paul E Abraham, Hengfu Yin, Anne M Borland, Deborah Weighill, Sung Don Lim, Henrique Cestari De Paoli, Nancy Engle, Piet C Jones, Ryan Agh, David J Weston, Stan D Wullschleger, Timothy Tschaplinski, Daniel Jacobson, John C Cushman, Robert L Hettich, Gerald A Tuskan, Xiaohan Yang
Already a proven mechanism for drought resilience, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency by means of an inverse (compared to C3 and C4 photosynthesis) day/night pattern of stomatal closure/opening to shift CO2 uptake to the night, when evapotranspiration rates are low. A systems-level understanding of temporal molecular and metabolic controls is needed to define the cellular behaviour underpinning CAM. Here, we report high-resolution temporal behaviours of transcript, protein and metabolite abundances across a CAM diel cycle and, where applicable, compare the observations to the well-established C3 model plant Arabidopsis...
November 21, 2016: Nature Plants
https://www.readbyqxmd.com/read/27853904/overexpression-of-smorf-ynr034w-a-ego4-in-saccharomyces-cerevisiae-increases-the-fermentative-efficiency-of-agave-tequilana-weber-must
#17
Naurú Idalia Vargas-Maya, Gloria Angélica González-Hernández, Israel Enrique Padilla-Guerrero, Juan Carlos Torres-Guzmán
Fermentative processes are widely used to produce food, beverages and biofuels. Saccharomyces cerevisiae is an efficient ethanol-producing microorganism. However, a concentration of high ethanol and other metabolites can affect yeast viability and decrease the ethanol yield. Many studies have focused on improving the fermentative efficiency, mostly through the genetic engineering of genes that have a direct impact on specific metabolic pathways. In the present study, we characterized a small open reading frame encoding a protein with an unknown function and biological role termed YNR034W-A...
November 16, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27792720/-effect-of-simvastatin-plus-inulin-in-comparison-with-simvastatin-plus-ezetimibe-on-the-treatment-of-mixed-dyslipidemia
#18
Esperanza Martínez-Abundis, Carmelita Barrera-Durán, Manuel González-Ortiz, Eduardo Hernández-Salazar
INTRODUCTION: Mixed dyslipidemia accelerates atherosclerosis and leads to cardiovascular disease and death. Non-soluble fibers such as inulin have been shown to have an effect on dyslipidemia. AIM: To assess the effect of the combination of simvastatin plus inulin in comparison with simvastatin plus ezetimibe in mixed dyslipidemia. MATERIAL AND METHODS: A randomized, double-blind, clinical trial with parallel control group was performed in 60 patients with mixed dyslipidemia, without drug treatment or failure to statins and lifestyle changes...
October 2016: Gaceta Médica de México
https://www.readbyqxmd.com/read/27786154/spectroscopic-and-microscopic-study-of-peroxyformic-pulping-of-agave-waste
#19
Hilda M Hernández-Hernández, Jorge J Chanona-Pérez, Alberto Vega, Pablo Ligero, Reynold R Farrera-Rebollo, Jorge A Mendoza-Pérez, Georgina Calderón-Domínguez, Norma Güemes Vera
The peroxyformic process is based on the action of a carboxylic acid (mainly formic acid) and the corresponding peroxyacid. The influences of processing time (60-180 min), formic acid concentration (80-95%), temperature (60-80°C), and hydrogen peroxide concentration (2-4%) on peroxyformic pulping of agave leaves were studied by surface response methodology using a face-centered factorial design. Empirical models were obtained for the prediction of yield, κ number (KN) and pulp viscosity as functions of the aforementioned variables...
October 2016: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/27777945/the-effects-of-agave-fourcroydes-powder-as-a-dietary-supplement-on-growth-performance-gut-morphology-concentration-of-igg-and-hematology-parameters-in-broiler-rabbits
#20
Maidelys Iser, Yordan Martínez, Hengjia Ni, Hongmei Jiang, Manuel Valdivié Navarro, Xiaosong Wu, Naif Abdullah Al-Dhabi, Manuel Rosales, Veeramuthu Duraipandiyan, Jun Fang
This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured...
2016: BioMed Research International
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