keyword
https://read.qxmd.com/read/38330520/developing-a-detection-strategy-for-ten-paralytic-shellfish-poisonings-in-urine-combining-high-throughput-desi-ms-screening-and-accurate-uplc-qqq-ms-quantification
#21
JOURNAL ARTICLE
Jing Cai, Shiyan Li, Qingcheng Wang, Dan Deng, Shitong Wang, Lijun Ge, Yiwei Cui, Yuejian Shen, Qing Shen
Paralytic shellfish poisoning (PSP) is the most widespread and harmful form of shellfish poisoning with high mortality rate. In this study, a combined desorption electrospray ionization mass spectrometry (DESI-MS) and ultra-performance liquid chromatography triple quadrupole mass spectrometry (UPLC-QqQ/MS) method was established for the detection of PSPs in urine. The method was optimized using a spray solution of methanol and water (1:1, v/v) containing 0.1 % FA, at a flow rate of 2.5 µL·min-1 and an applied voltage of 3 kV...
February 1, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38330519/a-liquid-chromatographic-mass-spectrometric-procedure-for-analysis-of-pentaerythrityl-tetranitrate-metabolites-development-validation-and-application-to-ovine-serum-and-human-plasma-samples
#22
JOURNAL ARTICLE
Mariann Städtler, Daniela Wissenbach, Dirk K Wissenbach, Laura Franke, Jana Pastuschek, Ekkehard Schleussner, Beth Allison, Frank T Peters, Tanja Groten
Pentaerythrityl tetranitrate (PETN) is an established drug in the treatment of coronary heart disease and heart failure. It is assumed, that the vasodilative and vasoprotective effects of PETN also have a positive impact on pregnant patients with impaired placental perfusion and studies evaluating the effect of PETN in risk pregnancies have been carried out. In the context of these clinical trials, measuring of serum levels of PETN and its metabolites pentaerythrityl trinitrate (PETriN), pentaerythrityl dinitrate (PEDN), pentaerythrityl mononitrate (PEMN) and pentaerythritol (PE) were required...
January 24, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38324182/discovery-and-exploration-of-lipid-modifying-drug-targets-for-als-by-mendelian-randomization
#23
JOURNAL ARTICLE
Zheng Jiang, Xiao-Jing Gu, Wei-Ming Su, Qing-Qing Duan, Kang-Fu Yin, Yan-Lin Ren, Yi Wang, Bei Cao, Yong-Ping Chen
Observational studies have faced challenges in identifying replicable causes for amyotrophic lateral sclerosis (ALS). To address this, we employed an unbiased and data-driven approach to discover and explore potential causal exposures using two-sample Mendelian randomization (MR) analyses. In the phenotype discovery stage, we assessed 3948 environmental exposures from the UK Biobank and utilized ALS summary statistics (Europeans, 20,806 cases, 59,804 controls) as the outcome within a phenome-wide MR pipeline...
February 7, 2024: Molecular Neurobiology
https://read.qxmd.com/read/38320436/purification-of-pineapple-bromelain-by-imac-chromatography-using-chlorophyll-activated-macroporous-matrices
#24
JOURNAL ARTICLE
Márjorie Castro Pinto Porfirio, Jonathan Barbosa Santos, Annie Nolasco Alves, Leandro Soares Santos, Renata Cristina Ferreira Bonomo, Rafael da Costa Ilhéu Fontan
This study investigated the purification of bromelain obtained from pineapple fruit using a new adsorbent for immobilized metal ion affinity chromatography (IMAC), with chlorophyll obtained from plant leaves as a chelating agent. The purification of bromelain was evaluated in batches from the crude extract of pineapple pulp (EXT), and the extract precipitated with 50 % ammonium sulfate (EXT.PR), the imidazole buffer (200 mM, pH 7.2) being analyzed and sodium acetate buffer, pH 5.0 + 1.0 NaCl as elution solutions...
January 22, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38310833/an-integrating-strategy-for-systematic-profiling-of-chinese-patent-drug-s-chemicalome-and-associated-metabolome-huanghou-antidiarrhea-dropping-pills-as-a-case-study
#25
JOURNAL ARTICLE
Li Li, Xuguang Wang, Ruiting Ma, Mingxing Hou
Huanghou antidiarrhea dropping pills (HADP) is an efficient Chinese patent drug that is clinically used to treat diarrhea. However, its functional materials remain unclear due to the characteristics of traditional Chinese medicine, which is a multi-component and multi-target complex system. In this study, we investigated the intrinsic chemical components and combined with in vivo metabolism to reveal the functional material basis of HADP. Spectral behavior (accurate molecular weight and secondary fragmentation) and chromatographic behavior (retention time) were key criterions that throughout the whole research of components identification, prototypes screening, and tissue distribution...
February 1, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38309826/gut-microbiota-derived-secondary-bile-acids-bile-acids-receptor-polymorphisms-and-risk-of-cardiovascular-disease-in-individuals-with-newly-diagnosed-type-2-diabetes-a-cohort-study
#26
JOURNAL ARTICLE
Qi Lu, Junxiang Chen, Limiao Jiang, Tingting Geng, Shufan Tian, Yunfei Liao, Kun Yang, Yan Zheng, Meian He, Huiru Tang, An Pan, Gang Liu
BACKGROUND: Secondary bile acids (SBAs), the products of bacterial metabolism, are ligands of the nuclear farnesoid X receptor (FXR) and have been implicated in cardiovascular health. Diet can modulate gut microbiota composition and bile acid metabolism. OBJECTIVES: We aimed to examine the associations of circulating SBAs and their receptor polymorphisms with the risk of incident cardiovascular disease (CVD) among people with type 2 diabetes (T2D). METHODS: A total of 1234 participants with newly diagnosed T2D without CVD or cancer were included from the Dongfeng-Tongji Cohort study in China...
February 2024: American Journal of Clinical Nutrition
https://read.qxmd.com/read/38309045/high-performance-liquid-chromatographic-method-optimized-by-box-behnken-design-model-to-determine-caffeine-in-pharmaceutical-preparations-and-urine-samples
#27
JOURNAL ARTICLE
Syed Najmul Hejaz Azmi, Wafa Aqib Nasir Al Rawahi, Arwa Ibrahim Al Yahyai, Asma Ali Al Qasimi, Kothar Saif Al Fuliti, Omayma Said Al Qalhati, Nafisur Rahman, Qamar Uddin Ahmed
A UV-HPLC method optimized by Box-Behnken design model was developed to determine caffeine in pharmaceutical preparations and urine samples. The chromatographic conditions followed were mobile phase: methanol/water/ citrate buffer (pH 4.6) (40:25:35, v/v/v),AcclaimTM Dionex C18 column (ODS 100A˚, 5 µm; 4.6 × 250 mm),flow rate (0.9 mL min-1 ), column temperature (30 °C) and UV-detection wavelength (204 nm). The chromatographic variables: pH (A), % methanol fraction (B), flow rate(C) and column temperature (D) were optimized at 50 μg mL-1 caffeine using BBD model...
February 1, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38309044/quantification-of-mirtazapine-in-tablets-via-dna-binding-mechanism-development-of-a-new-hplc-method
#28
JOURNAL ARTICLE
Ecem Kuzpınar, Abdullah Al Faysal, Pelin Şenel, Taner Erdoğan, Ayşegül Gölcü
Atypical antidepressant mirtazapine (MIR) is mostly prescribed for the management of major depressive disorder. The identification of MIR in pharmaceutical dosage forms was made possible by developing a novel, quick, sensitive high-performance liquid chromatography (HPLC) approach that was verified in accordance with ICH recommendations. In the first part of this study, HPLC investigations were optimized with regard to variables including pH, working column, mobile phase, temperature, and flow rate. The limit of detection (LOD) was 0...
January 26, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38309043/determination-of-sulfadoxine-and-pyrimethamine-in-microvolume-human-plasma-using-ultra-high-performance-liquid-chromatography-tandam-mass-spectrometry
#29
JOURNAL ARTICLE
Vong Sok, Florence Marzan, Michelle Roh, Kevin Guo, Jenny Legac, Norah Mwebaza, Grant Dorsey, Philip J Rosenthal, Francesca T Aweeka, Liusheng Huang
To support the pharmacokinetic study of sulfadoxine (SD) and pyrimethamine (PM) in pregnant women and children, sensitive methods with small sample volume are desirable. Here we report a method to determine SD and PM with microvolume plasma samples: 5 µL plasma samples were cleaned up by protein precipitation with acetonitrile. The deuterated analytes were used as the internal standards. The samples after cleanup were injected onto an ACE Excel SuperC18 column (50 × 2.1 mm, 1.7 μm, Hichrom Limited) connected to a Waters I class UPLC coupled with a Sciex Triple Quad 6500+ Mass Spectrometer and eluted with water and acetonitrile both containing 0...
January 24, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38306956/investigation-of-low-temperature-partitioning-with-dispersive-solid-phase-extraction-for-quantification-of-pesticides-in-apples-followed-by-electrospray-ionization-mobility-spectrometry-comparison-with-conventional-procedure
#30
JOURNAL ARTICLE
Wen Liu, Fei Liu, Anyi Che, Yanjing Chen, Jiayi Cai, Wenjie Liu, Guoxing Jing, Wenshan Li, Jianna Yu
Ion mobility spectrometry (IMS) has a promising application prospect in food surveillance. However, due to the complexity of food matrix and trace levels of pesticide residues, the effective and rapid detection of pesticides by IMS has been a challenge, especially when using electrospray ionization (ESI) as an ion source. In this study, low-temperature partitioning with dispersive solid-phase extraction (LTP-dSPE) was explored and compared with conventional procedures. Both methods were validated for the quantification of eight pesticides in apples, obtaining a limit of detection (LOD) of 0...
January 26, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38306955/a-novel-method-for-the-accurate-quantification-of-two-isomeric-mercapturic-acids-of-1-3-dichlorobenzene-in-human-urine-using-isotope-dilution-online-spe-lc-ms-ms
#31
JOURNAL ARTICLE
T Schettgen, V Belov, T Kraus, P Ziegler
1,3-dichlorobenzene (1,3-DCB) is an aromatic solvent that might be formed during thermal decomposition of bis(2,4-dichlorobenzoyl)peroxide used as initiator in silicone rubber production with many workers exposed worldwide. During metabolism of 1,3-DCB, two isomeric mercapturic acids can be formed from ring oxidation of 1,3-DCB in the liver, namely 2,4-dichlorophenylmercapturic acid (24CPhMA) and 3,5-dichlorophenylmercapturic acid (35CPhMA). These urinary mercapturic acids might serve as biomarkers of the toxicologically relevant absorbed dose of 1,3-DCB and have not been determined so far...
January 27, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38301337/a-probe-of-new-molecularly-imprinted-solid-phase-extraction-coupled-with-hplc-dad-and-atomic-absorption-spectrophotometry-for-quantification-of-tetracycline-hcl-metronidazole-and-bismuth-subcitrate-in-combination-with-their-official-impurities-application-in
#32
JOURNAL ARTICLE
Amal M Hassan, Khadiga M Kelani, Maha A Hegazy, Ahmed H Nadim, Mahmoud A Tantawy
The integration of molecular imprinting technique with chromatographic one has a great impact on the assay's selectivity and sensitivity. Herein, a molecularly imprinted solid-phase extraction associated with high performance liquid chromatography (MISPE-HPLC) was employed for simultaneous determination of the co-formulated drugs; tetracycline hydrochloride (TET) and metronidazole (MET), in plasma and in their anti-H-pylori drug for the first time. Two sorts of molecularly imprinted polymers (MIPs) were fabricated using TET and MET as the template molecules, while ethylene glycol dimethacrylate and methacrylic acid were used as a cross-linker and a monomer, respectively...
January 26, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38295722/facial-on-line-enrichment-of-glycoproteins-by-capillary-electrophoresis-with-boronate-functionalized-poly-glycidyl-methacrylate-microparticles-coated-column
#33
JOURNAL ARTICLE
Jian Zhang, Yanqing Miao, Hui Jing, Jingwen Wu, Chunye Liu
A facial and rapid method for glycoproteins enrichment by capillary electrophoresis was developed. The 3-aminophenylboronic acid-functionalized poly(glycidyl methacrylate) microparticles (PGMA@APBA) were attached to the capillary inlet (length of ∼1.5 cm) by electrostatic self-assemble action to prepare a partially coated capillary column. The process is simple and reversible, allowing for easy renewal of the PGMA@APBA coating when its enrichment efficiency decreases. By utilizing the coated column, glycoproteins can be enriched within 2 min...
January 26, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38293935/aspirin-and-cardiovascular-risk-in-individuals-with-elevated-lipoprotein-a-the-multi-ethnic-study-of-atherosclerosis
#34
JOURNAL ARTICLE
Harpreet S Bhatia, Patrick Trainor, Samantha Carlisle, Michael Y Tsai, Michael H Criqui, Andrew DeFilippis, Sotirios Tsimikas
BACKGROUND: Effective therapies for reducing cardiovascular disease (CVD) risk in people with elevated lipoprotein(a) are lacking, especially for primary prevention. Because of the potential association of lipoprotein(a) with thrombosis, we evaluated the relationship between aspirin use and CVD events in people with elevated lipoprotein(a). METHODS AND RESULTS: We used data from the MESA (Multi-Ethnic Study of Atherosclerosis), a prospective cohort study of individuals free of baseline cardiovascular disease...
January 31, 2024: Journal of the American Heart Association
https://read.qxmd.com/read/38286102/hollow-fiber-liquid-phase-microextraction-for-determination-of-fluoxetine-in-human-serum-by-nano-liquid-chromatography-coupled-to-high-resolution-mass-spectrometry
#35
JOURNAL ARTICLE
Beatriz Isabella Cestaro, Kelly Cavalcanti Machado, Michel Batista, Bruno José Gonçalves da Silva
Therapeutic drug monitoring (TDM) is a personalized care tool based on the determination of a target drug concentration in human serum. An antidepressant drug of interest for such investigations is fluoxetine (FXT), due to a severe impact of genetic polymorphisms on its metabolism. A bioanalytical method employed for TDM purposes must exhibit satisfactory selectivity and detectability, which becomes more difficult due to highly complex biological matrices. In this study, a highly selective bioanalytical method for the determination of FXT in human serum is proposed, which provides excellent clean-up efficiency based on a low cost hollow fiber liquid-phase microextraction (HF-LPME) sample preparation step and nano-liquid chromatography coupled to high-resolution mass spectrometry (nano-LC-HRMS)...
January 17, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38281369/ultrasensitive-detection-of-imatinib-in-human-serum-using-a-gold-based-paper-sensor
#36
JOURNAL ARTICLE
Xiaoqian Jiang, Aihua Qu, Xinxin Xu, Hua Kuang, Liqiang Liu, Chuanlai Xu
Imatinib is the tyrosine kinase inhibitor of choice for the treatment of chronic myeloid leukemia and gastrointestinal stromal tumors. However, imatinib has drawbacks such as drug resistance and significant differences in pharmacokinetics within patients. Therefore, a colloidal gold-based immunochromatographic assay (CG-IA) was developed for measuring and monitoring imatinib in human serum. An imatinib derivative containing carboxyl groups was used for the synthesis of the immunogen, and 4-(4-methyl-1-piperazinylmethyl) benzoic acid was selected as the hapten for the heterologous coating antigen...
January 24, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38280362/a-lc-ms-ms-method-for-the-simultaneous-quantitative-determination-of-aldosterone-its-precursor-18-hydroxycorticosterone-and-its-metabolite-tetrahydroaldosterone-in-human-urine
#37
JOURNAL ARTICLE
Haohang Dong, Bin Hu, Wei Chen, Liping Wang, Huiying Sun, Jianfeng Zhou, Jianchun Chen, Baorong Chen, Zhenxia Du
Aldosterone (ALD), its precursor 18-hydroxycorticosterone (18-OHB) and its metabolite tetrahydroaldosterone (TH-ALD) are important biomarkers for the diagnosis of primary aldosteronism (PA). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly utilized in the detection of small molecules of hormones because it has advantages in terms of specificity and sensitivity. The objective of this study is to develop a new LC-MS/MS method for the simultaneous quantification of ALD (free), 18-OHB, and TH-ALD in human urine and attempt to diagnose primary aldosteronism using different indicators...
January 14, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38277723/screening-and-identification-of-antibacterial-components-in-artemisia-argyi-essential-oil-by-tlc-direct-bioautography-combined-with-comprehensive-2d-gc%C3%A2-%C3%A3-%C3%A2-gc-tofms
#38
JOURNAL ARTICLE
Weiyi Wen, Haiping Xiang, Huiyun Qiu, Jianwei Chen, Xuemin Ye, Lin Wu, Zhiqiang Chen, Shengqiang Tong
One of the primary components that contribute to Artemisia argyi 's effectiveness is essential oil, which has an exceptional antibacterial effect that has been well documented. The actual cause of its antibacterial activity and associated mechanism, however, are still not completely understood. For the first time, comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (2D GC × GC-TOFMS) and thin-layer chromatography-direct bioautography (TLC-DB) were employed to investigate its antibacterial components...
January 23, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38277722/analytical-methods-for-detecting-butane-propane-and-their-metabolites-in-biological-samples-implications-for-inhalant-abuse-detection
#39
JOURNAL ARTICLE
Jihyun Kim, Sanggil Choe, Ilchung Shin, Moonhee Jang, Jaesin Lee, Sang Beom Han
Worldwide, various inhalants are widely abused for recreational purposes, with butane and propane emerging as among the most commonly misused volatile substances, posing a significant risk of sudden death. The rapid elimination and oxidation of these highly volatile compounds upon inhalation necessitate the identification of butane and propane along with their metabolites in biological samples. Hence, the primary objective of this study is twofold: firstly, to establish a method for analyzing butane, propane, and metabolites, and secondly, to demonstrate the detection window and exposure indicators associated with the inhalation of butane and propane...
January 13, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38271747/enhancing-affinity-purification-of-monoclonal-antibodies-from-human-serum-for-subsequent-cze-ms-analysis
#40
JOURNAL ARTICLE
Tessa Reinert, Pascal Houzé, Yannis-Nicolas Francois, Rabah Gahoual
Due to the separation technique employed, capillary electrophoresis coupled to mass spectrometry (CE-MS) analysis performances are significantly influenced by the chemical composition and the complexity of the sample. In various applications, that impact has prevented the use of CE-MS for the characterization and quantification of proteins in biological samples. Here we present the development and evaluation and a sample preparation procedure, based on affinity purification, for the specific extraction of the monoclonal antibody (mAbs) infliximab from human serum in order to perform subsequent proteolytic digestion and CE-MS/MS analysis...
January 8, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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