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Gelsolin

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https://www.readbyqxmd.com/read/28794177/discovery-and-validation-of-a-six-marker-serum-protein-signature-for-the-diagnosis-of-active-pulmonary-tuberculosis
#1
Mary A De Groote, David G Sterling, Thomas Hraha, Theresa Russell, Louis S Green, Kirsten Wall, Stephan Kraemer, Rachel Ostroff, Nebojsa Janjic, Urs A Ochsner
New non-sputum biomarker tests for active tuberculosis (TB) diagnostics are of highest priority for global TB control. We performed in-depth proteomic analysis using the 4000-plex SOMAscan assay on 1470 serum samples from seven TB-endemic countries. All samples were from patients with symptoms and signs suggestive of active pulmonary TB that were systematically confirmed or ruled-out for TB by culture and clinical follow-up. HIV-coinfection was present in 34%and 22% were sputum smear-negative. Serum protein biomarkers were identified by stability selection using L1-regularized logistic regression and by Kolmogorov-Smirnov (KS) statistics...
August 9, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28791367/molecular-mechanisms-of-breast-cancer-metastasis-by-gene-expression-profile-analysis
#2
Tianying Zheng, Aijun Wang, Dongyan Hu, Yonggang Wang
Metastasis is the main cause of breast cancer‑related mortalities. The present study aimed to uncover the relevant molecular mechanisms of breast cancer metastasis and to explore potential biomarkers that may be used for prognosis. Expression profile microarray data GSE8977, which contained 22 stroma samples (15 were from normal breast and 7 were from invasive ductal carcinoma tumor samples), were obtained from the Gene Expression Omnibus database. Following data preprocessing, differentially expressed genes (DEGs) were selected based on analyses conducted using the linear models for microarray analysis package from R and Bioconductor software...
August 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28757820/nonconventional-markers-of-sepsis
#3
Péter Kustán, Zoltán Horváth-Szalai, Diána Mühl
Sepsis still remains a challenging healthcare problem with high mortality rate. To improve outcome, early diagnosis and monitoring of sepsis is of utmost importance. In this process objective laboratory parameters are the most helpful. Procalcitonin and C-reactive protein are the most commonly used and recommended markers of sepsis however, more than 200 sepsis biomarkers have already been published. This mini review focuses on nonconventional novel possibilities for the recognition of sepsis severity. Presepsin, actin and actin scavenger proteins (gelsolin and Gc-globulin) and orosomucoid are discussed...
May 2017: EJIFCC
https://www.readbyqxmd.com/read/28755273/cognitive-impairment-and-brain-and-peripheral-alterations-in-a-murine-model-of-intraventricular-hemorrhage-in-the-preterm-newborn
#4
Antonio Segado-Arenas, Carmen Infante-Garcia, Isabel Benavente-Fernandez, Daniel Sanchez-Sotano, Juan Jose Ramos-Rodriguez, Almudena Alonso-Ojembarrena, Simon Lubian-Lopez, Monica Garcia-Alloza
Germinal matrix hemorrhage-intraventricular hemorrhage (GMH-IVH) remains a serious complication in the preterm newborn. The significant increase of survival rates in extremelye preterm newborns has also contributed to increase the absolute number of patients developing GMH-IVH. However, there are relatively few available animal models to understand the underlying mechanisms and peripheral markers or prognostic tools. In order to further characterize central complications and evolution of GMH-IVH, we injected collagenase intraventricularly to P7 CD1 mice and assessed them in the short (P14) and the long term (P70)...
July 28, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28700355/identification-of-cd14-as-a-potential-biomarker-of-hepatocellular-carcinoma-using-itraq-quantitative-proteomics
#5
Jiao Guo, Rui Jing, Jian-Hong Zhong, Xin Dong, Yun-Xi Li, Yin-Kun Liu, Tian-Ren Huang, Chun-Yan Zhang
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors without effective diagnostic biomarkers. This study intended to dynamically analyze serum proteomics in different pathological stages of liver diseases, and discover potential diagnostic biomarkers for early HCC. Patients with hepatitis B virus (HBV) infection, liver cirrhosis (LC), or HCC together with healthy controls (HC) were enrolled. Proteins differentially expressed between groups were screened using isobaric tagging for relative and absolute quantitation (iTRAQ), and promising HCC biomarker candidates were subjected to bioinformatics analysis, including K-means clustering, gene ontology (GO) and string network analysis...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28680082/visualizing-temperature-mediated-activation-of-gelsolin-and-its-deactivation-by-pip2-a-saxs-based-study
#6
Maulik D Badmalia, Shikha Singh, Renu Garg, Ashish
This is the first report describing temperature based initiation of gelsolin's F-actin depolymerization activity, even in absence of free Ca(2+) or low pH. Small angle X-ray scattering (SAXS) and circular dichroism (CD) studies revealed that temperature in the range of 30-40 °C is capable of opening the G1 domain alone, as remaining domains are held together by the Ca(2+)-sensitive C-tail latch without any loss in the secondary structural content. Full opening of all domains of tail-less gelsolin, and retention of closed shape for G2-G6 gelsolin merely by heating, further substantiated our findings...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28673451/a-prospective-proteomics-study-identified-potential-biomarkers-of-encapsulating-peritoneal-sclerosis-in-peritoneal-effluent
#7
Vasileios Zavvos, Anthony T Buxton, Caroline Evans, Mark Lambie, Simon J Davies, Nicholas Topley, Martin Wilkie, Angela Summers, Paul Brenchley, Dimitrios S Goumenos, Timothy S Johnson
Encapsulating peritoneal sclerosis (EPS) is a potentially devastating complication of peritoneal dialysis (PD). Diagnosis is often delayed due to the lack of effective and accurate diagnostic tools. We therefore examined peritoneal effluent for potential biomarkers that could predict or confirm the diagnosis of EPS and would be valuable in stratifying at-risk patients and driving appropriate interventions. Using prospectively collected samples from the Global Fluid Study and a cohort of Greek PD patients, we utilized 2D SDSPAGE/ MS and iTRAQ to identify changes in the peritoneal effluent proteome from patients diagnosed with EPS and controls matched for treatment exposure...
July 1, 2017: Kidney International
https://www.readbyqxmd.com/read/28647184/identification-of-novel-biomarkers-for-adult-onset-immunodeficiency-aoid-syndrome-using-serum-proteomics
#8
Jeerang Wongtrakul, Thananya Thongtan, Sittiruk Roytrakul, Jutarat Praparattanapan, Jiraprapa Wipasa, Benjawan Kumrapich, Khuanchai Supparatpinyo
OBJECTIVE: To identify the candidate protein biomarkers of adult-onset-immunodeficiency (AOID) syndrome using serum proteomics. METHODS: Screening and verification phases were performed in the study. A total of 97 serum samples were classified into three groups: AOID patients with opportunistic infections (active AOID), AOID patients without opportunistic infections (inactive AOID), and healthy control. In the screening phase, pooled sera collected from patients and healthy control in each group were separated by 2D-gel electrophoresis, analyzed for differentially expressed proteins and identified for biomarkers using LC/MS...
May 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28640320/molecular-signature-of-coronary-stent-thrombosis-oxidative-stress-and-innate-immunity-cells
#9
Judit Cubedo, Ana Blasco, Teresa Padro, Ilaria Ramaiola, Oriol Juan-Babot, Javier Goicolea, José Antonio Fernández-Díaz, Juan Francisco Oteo, Lina Badimon
The clinical impact of in-stent thrombosis is high because it is associated with high mortality and 20 % of the patients suffer a recurrent event within the two following years. The aim of this study was to characterise the morphologic and proteomic profile of in-stent thrombi (IST) in comparison to thrombi developed on native coronary arteries (CT) to identify a differential molecular signature. The study included 45 patients with ST-elevation-myocardial-infarction (STEMI) treated by primary-percutaneous-intervention and thrombus aspiration: 21 had IST and 24 had CT...
June 22, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28622474/larch-arabinogalactan-attenuates-myocardial-injury-by-inhibiting-apoptotic-cascades-in-a-rat-model-of-ischemia-reperfusion
#10
Sun-Ha Lim
We reported previously that supplementation with apple pectin, a dietary fiber, reduced myocardial injury in a rat model of ischemia-reperfusion. In this study, we further investigated an arabinogalactan, one of the constituent polysaccharides of pectin, to determine which domains comprising pectin were responsible for the protection. In a rat model of 30-min ischemia followed by 3-h reperfusion, supplementation with larch arabinogalactan (LAG) over 50 mg/kg/day significantly reduced infarct size. Reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry, and immunoblot analyses showed that intake of LAG blocked the steps involved in apoptotic cascades through downregulation of gelsolin gene expression at the protein (Gelsolin) level, inhibition of p38 phosphorylation in mitogen-activated protein kinase (MAPK) pathways, decreased bax/bcl-2 ratio at the protein (Bax/Bcl-2) level, which was correlated with the ratio at the mRNA level, inhibition of the conversion of Procaspase protein to Caspase-3 protein, and consequently a decrease in apoptotic cells...
July 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28605435/aav9-delivered-bispecific-nanobody-attenuates-amyloid-burden-in-the-gelsolin-amyloidosis-mouse-model
#11
Adriaan Verhelle, Nisha Nair, Inge Everaert, Wouter Van Overbeke, Lynn Supply, Olivier Zwaenepoel, Cindy Peleman, Jo Van Dorpe, Tony Lahoutte, Nick Devoogdt, Wim Derave, Marinee K Chuah, Thierry VandenDriessche, Jan Gettemans
No abstract text is available yet for this article.
June 9, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28599499/comparative-secretome-of-ovarian-serous-carcinoma-gelsolin-in-the-spotlight
#12
Sandra Pierredon, Pascale Ribaux, Jean-Christophe Tille, Patrick Petignat, Marie Cohen
Ovarian cancer is one of the most common types of reproductive cancer, and has the highest mortality rate amongst gynecological cancer subtypes. The majority of ovarian cancers are diagnosed at an advanced stage, resulting in a five-year survival rate of ~30%. Early diagnosis of ovarian cancer has improved the five-year survival rate to ≥90%, thus the current imperative requirement is to identify biomarkers that would allow the early detection, diagnosis and monitoring of the progression of the disease, or of novel targets for therapy...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28582578/proteomics-of-the-epicardial-fat-secretome-and-its-role-in-post-operative-atrial-fibrillation
#13
Alessandro Viviano, Xiaoke Yin, Anna Zampetaki, Marika Fava, Mark Gallagher, Manuel Mayr, Marjan Jahangiri
Aims: Post-operative atrial fibrillation (POAF) is a predictor of morbidity and mortality after cardiac surgery. Latent predisposing factors may reside in the epicardial adipose tissue (EAT) due to its anatomical position and high protein production rate. In order to explore a possible mechanistic link, we characterized proteins secreted by the EAT preceding the onset of POAF. Methods and results: Epicardial adipose tissue samples were collected from 76 consecutive patients with no history of AF undergoing coronary artery bypass surgery, 50 samples for proteomic analysis and 26 for gene expression studies, further divided according to development of POAF...
June 5, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28574190/flow-aligned-single-shot-fiber-diffraction-using-a-femtosecond-x-ray-free-electron-laser
#14
David Popp, N Duane Loh, Habiba Zorgati, Umesh Ghoshdastider, Lu Ting Liow, Magdalena I Ivanova, Mårten Larsson, Daniel P DePonte, Richard Bean, Kenneth R Beyerlein, Cornelius Gati, Dominik Oberthuer, David Arnlund, Gisela Brändén, Peter Berntsen, Duilio Cascio, Leonard M G Chavas, Joe P J Chen, Ke Ding, Holger Fleckenstein, Lars Gumprecht, Rajiv Harimoorthy, Estelle Mossou, Michael R Sawaya, Aaron S Brewster, Johan Hattne, Nicholas K Sauter, Marvin Seibert, Carolin Seuring, Francesco Stellato, Thomas Tilp, David S Eisenberg, Marc Messerschmidt, Garth J Williams, Jason E Koglin, Lee Makowski, Rick P Millane, Trevor Forsyth, Sébastien Boutet, Thomas A White, Anton Barty, Henry Chapman, Swaine L Chen, Mengning Liang, Richard Neutze, Robert C Robinson
A major goal for X-ray free-electron laser (XFEL) based science is to elucidate structures of biological molecules without the need for crystals. Filament systems may provide some of the first single macromolecular structures elucidated by XFEL radiation, since they contain one-dimensional translational symmetry and thereby occupy the diffraction intensity region between the extremes of crystals and single molecules. Here, we demonstrate flow alignment of as few as 100 filaments (Escherichia coli pili, F-actin, and amyloid fibrils), which when intersected by femtosecond X-ray pulses result in diffraction patterns similar to those obtained from classical fiber diffraction studies...
June 2, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/28573081/comparative-proteomic-analysis-of-rat-bronchoalveolar-lavage-fluid-after-exposure-to-zinc-oxide-nanoparticles
#15
Yu-Min Juang, Han-Ju Chien, Cheng-Yu Yang, Hsiao-Chien Yeh, Tsun-Jen Cheng, Chien-Chen Lai
Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanomaterials in consumer products and industrial applications. As a result of all these uses, this has raised concerns regarding their potential toxicity. We previously found that candidate markers of idiopathic pulmonary fibrosis and lung cancer were significantly up-regulated in rat bronchoalveolar lavage fluid (BALF) following exposure to ZnO NPs by using a liquid chromatography (LC)-based proteomic approach. To achieve comprehensive protein identification analysis, we conducted the two-dimensional gel electrophosis (2-DE)-based proteomic workflow to analyze the differences in BALF proteins from rats that had been exposed to a high dose of 35 nm ZnO NPs...
2017: Mass Spectrometry
https://www.readbyqxmd.com/read/28500241/a-plague-of-actin-disassembly
#16
Shoichiro Ono
Pathogenic Yersinia species employ several strategies to evade the host immune system, including interfering with cytoskeletal remodeling as a way to block macrophage phagocytosis. The kinase YopO binds directly to monomeric actin and phosphorylates the actin-remodeling protein gelsolin, but the functional importance of this gelsolin modification has not been clear. A combined biochemical, computational, and biophysical study now reveals that YopO-mediated phosphorylation activates host gelsolin, leading to severed actin filaments and disturbed actin dynamics...
May 12, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28498470/novel-role-of-semaphorin%C3%A2-3a-in-the-growth-and-progression-of-hepatocellular-carcinoma
#17
Xuedong Li, Qing Chen, Dan Yin, Shiming Shi, Lei Yu, Shaolai Zhou, Erbao Chen, Zhengjun Zhou, Yinghong Shi, Jia Fan, Jian Zhou, Zhi Dai
Semaphorin 3A (SEMA3A), a secretory protein, is a founding member of the semaphorin family and functions in both the biological behavior of tumor cells and the modulation of tumor-associated macrophages. However, the role of SEMA3A in hepatocellular carcinoma (HCC) is still not well established. In the present study, we investigated the expression levels of SEMA3A in 80 HCC tissues and cell lines, using RT-qPCR, western blotting and immunohistochemistry. Expression profile analysis revealed that SEMA3A was significantly overexpressed in human HCC patients and positively correlated with the metastatic potential of HCC cells...
May 2, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28498392/the-flightless-i-protein-interacts-with-rna-binding-proteins-and-is-involved-in-the-genome-wide-mrna-post-transcriptional-regulation-in-lung-carcinoma-cells
#18
Cuihua Wang, Kezhi Chen, Shengyou Liao, Wei Gu, Xinlei Lian, Jing Zhang, Xuejuan Gao, Xiaohui Liu, Tong Wang, Qing-Yu He, Gong Zhang, Langxia Liu
The flightless I protein (FLII) belongs to the gelsolin family. Its function has been associated with actin remodeling, embryonic development, wound repair, and more recently with cancer. The structure of FLII is characterized by the N-terminal leucine-rich repeats (LRR) and C-terminal gesolin related repeated units that are both protein-protein inter-action domains, suggesting that FLII may exert its function by interaction with other proteins. Therefore, systematic study of protein interactions of FLII in cells is important for the understanding of FLII functions...
May 11, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28469748/plasma-actin-gelsolin-and-orosomucoid-levels-after-eccentric-exercise
#19
Éva Tékus, Márk Váczi, Zoltán Horváth-Szalai, Andrea Ludány, Tamás Kőszegi, Márta Wilhelm
The present study investigated the acute effect of eccentric exercise on blood plasma actin, gelsolin (GSN) and orosomucoid (AGP) levels in untrained and moderately trained individuals, and their correlation with exercise induced muscle damage (EIMD) markers (CK, intensity of muscle soreness and maximal voluntary contraction torque deficit). Healthy physical education students (6 untrained, 12 moderately trained) participated in this research. Actin, GSN, AGP and CK levels were measured in blood plasma at baseline, immediately, 1 h, 6 h and 24 h post-exercise comprising 90 eccentric quadriceps contractions performed on a dynamometer...
February 2017: Journal of Human Kinetics
https://www.readbyqxmd.com/read/28465181/expression-purification-and-characterization-of-recombinant-8%C3%A2-kda-gelsolin-fragment
#20
Qing Zhang, Weijie Lu, Lina Ji, Zi-Chun Hua
A mutation (D187N/Y) in human plasma gelsolin (GSN) leads to the generation of an 8 kDa GSN fragment (8 kDa-GSN), and consequently causes the familial amyloidosis of Finnish type. Because of its faster kinetics of amyloid formation under physiologically relevant conditions, 8 kDa-GSN is used to explore gelsolin amyloidosis and screen small molecules that can disaggregate amyloids. However, the synthetic 8 kDa-GSN is expensive, and substantial quantities of 8 kDa-GSN are needed for the screen. Here we report a study to obtain recombinant 8 kDa-GSN with high yield from Escherichia coli...
July 2017: Protein Expression and Purification
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