keyword
MENU ▼
Read by QxMD icon Read
search

Gelsolin

keyword
https://www.readbyqxmd.com/read/29332824/dithiothreitol-based-protein-equalization-technology-to-unravel-biomarkers-for-bladder-cancer
#1
J E Araújo, H López-Fernández, M S Diniz, Pedro M Baltazar, Luís Campos Pinheiro, Fernando Calais da Silva, Mylène Carrascal, Paula Videira, H M Santos, J L Capelo
This study aimed to assess the benefits of dithiothreitol (DTT)-based sample treatment for protein equalization to assess potential biomarkers for bladder cancer. The proteome of plasma samples of patients with bladder carcinoma, patients with lower urinary tract symptoms (LUTS) and healthy volunteers, was equalized with dithiothreitol (DTT) and compared. The equalized proteomes were interrogated using two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29320362/predictive-value-of-serum-gelsolin-and-gc-globulin-in-sepsis-a-pilot-study
#2
Zoltán Horváth-Szalai, Péter Kustán, Balázs Szirmay, Ágnes Lakatos, Per Hjort Christensen, Tamás Huber, Beáta Bugyi, Diána Mühl, Andrea Ludány, Attila Miseta, Gábor L Kovács, Tamás Kőszegi
BACKGROUND: Simultaneous determination of the two main actin scavenger proteins in sepsis has not been investigated until now. In our pilot study, we elucidated the predictive values of Gc globulin and gelsolin (GSN) in sepsis by comparing them to classic laboratory and clinical parameters. METHODS: A 5-day follow-up was performed, including 46 septic patients, 28 non-septic patients and 35 outpatients as controls. Serum Gc globulin and GSN levels were determined by automated immune turbidimetric assay on a Cobas 8000/c502 analyzer...
January 10, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29274870/villin-1-and-gelsolin-regulate-changes-in-actin-dynamics-that-affect-cell-survival-signaling-pathways-and-intestinal-inflammation
#3
Swati Roy, Amin Esmaeilniakooshkghazi, Srinivas Patnaik, Yaohong Wang, Sudeep P George, Afzal Ahrorov, Jason K Hou, Allan J Herron, Hiromi Sesaki, Seema Khurana
BACKGROUND & AIMS: Cell stress signaling pathways result in phosphorylation of the eukaryotic translation initiation factor 2 subunit alpha (EIF2S1 or EIF2A), which affects regulation of protein translation. Translation reprogramming mitigates stress by activating pathways that result in autophagy and cell death, to eliminate damaged cells. Actin is modified during stress and EIF2A is dephosphorylated to restore homeostasis. It is not clear how actin affects EIF2A signaling. We studied the actin-binding proteins villin 1 (VIL1) and gelsolin (GSN) in intestinal epithelial cells (IECs) to determine whether they respond to cell stress response and affect signaling pathways...
December 21, 2017: Gastroenterology
https://www.readbyqxmd.com/read/29220409/acquisition-of-functions-on-the-outer-capsid-surface-during-evolution-of-double-stranded-rna-fungal-viruses
#4
Carlos P Mata, Daniel Luque, Josué Gómez-Blanco, Javier M Rodríguez, José M González, Nobuhiro Suzuki, Said A Ghabrial, José L Carrascosa, Benes L Trus, José R Castón
Unlike their counterparts in bacterial and higher eukaryotic hosts, most fungal viruses are transmitted intracellularly and lack an extracellular phase. Here we determined the cryo-EM structure at 3.7 Å resolution of Rosellinia necatrix quadrivirus 1 (RnQV1), a fungal double-stranded (ds)RNA virus. RnQV1, the type species of the family Quadriviridae, has a multipartite genome consisting of four monocistronic segments. Whereas most dsRNA virus capsids are based on dimers of a single protein, the ~450-Å-diameter, T = 1 RnQV1 capsid is built of P2 and P4 protein heterodimers, each with more than 1000 residues...
December 8, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29216917/unexpected-profile-of-sphingolipid-contents-in-blood-and-bone-marrow-plasma-collected-from-patients-diagnosed-with-acute-myeloid-leukemia
#5
Marzena Wątek, Bonita Durnaś, Tomasz Wollny, Marcin Pasiarski, Stanisław Góźdź, Michał Marzec, Anna Chabowska, Przemysław Wolak, Małgorzata Żendzian-Piotrowska, Robert Bucki
BACKGROUND: Impaired apoptotic pathways in leukemic cells enable them to grow in an uncontrolled way. Moreover, aberrations in the apoptotic pathways are the main factor of leukemic cells drug resistance. METHODS: To assess the presence of potential abnormalities that might promote dysfunction of leukemic cells growth, HPLC system was used to determine sphingosine (SFO), sphinganine (SFA), sphingosine-1-phosphate (S1P) and ceramide (CER) concentration in the blood collected from patients diagnose with acute myeloblastic leukemia (AML; n = 49) and compare to values of control (healthily) group (n = 51)...
December 8, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29175858/gelsolin-regulates-proliferation-apoptosis-and-invasion-in-nk-t-cell-lymphoma-cells
#6
Yanwei Guo, Hongqiao Zhang, Xin Xing, Lijuan Wang, Jian Zhang, Lin Yan, Xiaoke Zheng, Mingzhi Zhang
The expression of gelsolin (GSN) is abnormal in many cancers, including extranodal nasal-type natural killer/T-cell lymphoma (NKTCL). However, the biological function of GSN and its mechanism in NKTCL remain unclear. We found GSN overexpression significantly suppressed cell proliferation, colony formationand invasion and promoted apoptosis of YTS cells. Moreover, the upregulation of GSN significantly decreased the protein levels of PI3K and p-AKT. Interestingly, blocking the PI3K/AKT signaling pathway significantly inhibited cell proliferation and invasion and promoted apoptosis of YTS cells...
November 24, 2017: Biology Open
https://www.readbyqxmd.com/read/29167514/common-origin-of-the-gelsolin-gene-variant-in-62-finnish-agel-amyloidosis-families
#7
Tuuli Mustonen, Eeva-Kaisa Schmidt, Miko Valori, Pentti J Tienari, Sari Atula, Sari Kiuru-Enari
Finnish gelsolin amyloidosis (AGel amyloidosis) is an autosomal dominantly inherited systemic disorder with ophthalmologic, neurologic and dermatologic symptoms. Only the gelsolin (GSN) c.640G>A variant has been found in the Finnish patients thus far. The purpose of this study was to examine whether the Finnish patients have a common ancestor or whether multiple mutation events have occurred at c.640G, which is a known mutational hot spot. A total of 79 Finnish AGel amyloidosis families including 707 patients were first discovered by means of patient interviews, genealogic studies and civil and parish registers...
November 22, 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/29148252/sex-hormone-binding-globulin-shbg-is-a-potential-early-diagnostic-biomarker-for-gastric-cancer
#8
Chao-Wen Cheng, Che-Chang Chang, Yudha Nur Patria, Ruei-Ting Chang, Yun-Ru Liu, Fu-An Li, Hsiu-Ming Shih, Ching-Yu Lin
The use of blood plasma biomarkers in gastric cancer (GC) management is limited due to a lack of reliable biomarkers. An LC-MS/MS assay and a bioinformatic analysis were performed to identify blood plasma biomarkers in a GC discovery cohort. The data obtained were verified and validated by western blotting and an ELISA in an independent study cohort. A label-free quantification analysis of the MS data using PEAKS7 software found that four plasma proteins of apolipoprotein C-1, gelsolin, sex hormone-binding globulin (SHBG), and complement component C4-A were significantly overexpressed in GC patients...
November 17, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/29142235/proteomic-study-revealed-cellular-assembly-and-lipid-metabolism-dysregulation-in-sepsis-secondary-to-community-acquired-pneumonia
#9
Narendra Kumar Sharma, Alexandre Keiji Tashima, Milena Karina Colo Brunialti, Eden Ramalho Ferreira, Ricardo Jose Soares Torquato, Renato Arruda Mortara, Flavia Ribeiro Machado, Murillo Assuncao, Otelo Rigato, Reinaldo Salomao
Sepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29105980/extracellular-vesicles-in-the-adaptive-process-of-prostate-cancer-during-inhibition-of-androgen-receptor-signalling-by-enzalutamide
#10
Carolina Soekmadji, Anja Rockstroh, Grant A Ramm, Colleen C Nelson, Pamela J Russell
Current treatments for advanced prostate cancer focus on inhibition of the androgen receptor (AR) by androgen deprivation therapy (ADT). However, complex interactions mediated by tumour suppressors, oncogenes, aberrations of AR expression, or de novo androgen production have been shown to induce the adaptive response of prostate cancer, leading to the development of castration resistant prostate cancer (CRPC). In this study, we report the effects of AR antagonist, enzalutamide on the protein contents of extracellular vesicles (EVs)...
November 6, 2017: Proteomics
https://www.readbyqxmd.com/read/29102633/actin-cytoskeleton-and-sperm-function
#11
Haim Breitbart, Maya Finkelstein
For the acquisition of the ability to fertilize the egg, mammalian spermatozoa should undergo a series of biochemical transformations in the female reproductive tract, collectively called capacitation. The capacitated sperm can undergo the acrosomal exocytosis process near or on the oocyte, which allows the spermatozoon to penetrate and fertilize it. One of the main processes in capacitation involves dynamic cytoskeletal remodeling particularly of actin. Actin polymerization occurs during sperm capacitation and the produced F-actin should be depolymerized prior to the acrosomal exocytosis...
November 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29100377/secreted-gelsolin-desensitizes-and-induces-apoptosis-of-infiltrated-lymphocytes-in-prostate-cancer
#12
Chun-Chi Chen, Shiow-Her Chiou, Cheng-Lin Yang, Kuan-Chih Chow, Tze-Yi Lin, Hui-Wen Chang, Weir-Chiang You, Hisu-Wen Huang, Chien-Min Chen, Nien-Cheng Chen, Fen-Pi Chou, Ming-Chih Chou
Loss of immunosurveillance is a major cause of cancer progression. Here, we demonstrate that gelsolin, a constituent of ejaculate, induces apoptosis of activated lymphocytes in prostate cancer. Gelsolin was highly expressed in prostate cancer cells, and was associated with tumor progression, recurrence, metastasis, and poor prognosis. In vitro, secreted gelsolin inactivated CD4(+) T cells by binding to CD37, and induced apoptosis of activated CD8(+) T lymphocytes by binding to Fas ligand during cell contact dependent on major histocompatibility complex I...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29074263/novel-subfamilies-of-actin-regulating-proteins
#13
A A Morozov, Ye D Bedoshvili, M S Popova, Ye V Likhoshway
Ability of actin to polymerise and depolymerise makes it essential to key functions of eukaryotic cell. The functioning of actin is controlled by a host of regulatory proteins, the repertoire of which in diatoms is known to remarkably differ from other organisms. We have performed a phylogenetic analysis of 521 actin and actin-related proteins' aminoacid sequences, as well as 190 sequences of gelsolin family proteins from various genomic and transcriptomic datasets. Based on the results of this analysis, as well as on the presence of clade-specific indels in some of the actin-related proteins, we describe a novel ARP subfamily, dubbed ARP12, which is specific to heterokonts and related organisms...
October 23, 2017: Marine Genomics
https://www.readbyqxmd.com/read/29069428/gelsolin-pathogenic-gly167arg-mutation-promotes-domain-swap-dimerization-of-the-protein
#14
Francesco Bonì, Mario Milani, Alberto Barbiroli, Luisa Diomede, Eloise Mastrangelo, Matteo de Rosa
AGel amyloidosis is a genetic degenerative disease characterized by the deposition of insoluble gelsolin protein aggregates in different tissues. Until recently, this disease was associated with two mutations of a single residue (Asp187 to Asn/Tyr) in the second domain of the protein. The general opinion is that pathogenic variants are not per se amyloidogenic but rather that the mutations trigger an aberrant proteolytic cascade, which results in the production of aggregation prone fragments. Here we report the crystal structure of the second domain of gelsolin carrying the recently identified Gly167Arg mutation...
October 23, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29068915/ocular-manifestations-of-systemic-amyloidosis
#15
Margaret M Reynolds, Kevin K Veverka, Morie A Gertz, Angela Dispenzieri, Steven R Zeldenrust, Nelson Leung, Jose S Pulido
PURPOSE: To describe ophthalmic manifestations of systemic amyloidosis, a group of devastating conditions. METHODS: A retrospective chart review including patients who had ocular examinations at Mayo Clinic between January 1, 1985, and April 1, 2014, and a diagnosis of light-chain (AL), secondary (AA), or nontransthyretin familial amyloidosis was undertaken. Sixty-eight patients with AL amyloidosis, eight patients with AA amyloidosis, and five patients with nontransthyretin familial amyloidosis were included...
October 24, 2017: Retina
https://www.readbyqxmd.com/read/29047238/identification-of-10-candidate-biomarkers-distinguishing-tuberculous-and-malignant-pleural-fluid-by-proteomic-methods
#16
Chang Youl Lee, Ji Young Hong, Myung Goo Lee, In Bum Suh
PURPOSE: Pleural effusion, an accumulation of fluid in the pleural space, usually occurs in patients when the rate of fluid formation exceeds the rate of fluid removal. The differential diagnosis of tuberculous pleurisy and malignant pleural effusion is a difficult task in high tuberculous prevalence areas. The aim of the present study was to identify novel biomarkers for the diagnosis of pleural fluid using proteomics technology. MATERIALS AND METHODS: We used samples from five patients with transudative pleural effusions for internal standard, five patients with tuberculous pleurisy, and the same numbers of patients having malignant effusions were enrolled in the study...
November 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/28993697/n-terminal-gelsolin-fragment-potentiates-trail-mediated-death-in-resistant-hepatoma-cells
#17
Keith Meyer, Young-Chan Kwon, Ratna B Ray, Ranjit Ray
TNF-α related apoptosis-inducing ligand (TRAIL) selectively kills tumor cells, without damaging normal cells. TRAIL receptors facilitate induction of apoptosis for selective elimination of malignant cells. However, some cancer cells have developed resistances to TRAIL which limits anticancer potential. Gelsolin, a multifunctional actin-binding protein, mediates cell death involving the TRAIL receptors in the hepatic stellate cell line, LX2. Here, we have shown that conditioned medium (CM) containing gelsolin fragments or an N-terminal gelsolin fragment (amino acid residues 1-70) in the presence of TRAIL impairs cell viability of TRAIL resistant transformed human hepatocytes (HepG2)...
October 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28977922/identification-of-cd14-as-a-potential-biomarker-of-hepatocellular-carcinoma-using-itraq-quantitative-proteomics
#18
Jiao Guo, Rui Jing, Jian-Hong Zhong, Xin Dong, Yun-Xi Li, Yin-Kun Liu, Tian-Ren Huang, Chun-Yan Zhang
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors without effective diagnostic biomarkers. This study intended to dynamically analyze serum proteomics in different pathological stages of liver diseases, and discover potential diagnostic biomarkers for early HCC. Patients with hepatitis B virus (HBV) infection, liver cirrhosis (LC), or HCC together with healthy controls (HC) were enrolled. Proteins differentially expressed between groups were screened using isobaric tagging for relative and absolute quantitation (iTRAQ), and promising HCC biomarker candidates were subjected to bioinformatics analysis, including K-means clustering, gene ontology (GO) and string network analysis...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28975596/erratum-to-hypoxia-augments-increased-hif-1a-and-reduced-survival-protein-p-akt-in-gelsolin-gsn-dependent-cardiomyoblast-cell-apoptosis
#19
Yu-Lan Yeh, Wei-Syun Hu, Wei-Jen Ting, Chia-Yao Shen, Hsi-Hsien Hsu, Li-Chin Chung, Chuan-Chou Tu, Sheng-Huang Chang, Cecilia-Hsuan Day, Yuhsin Tsai, Chih-Yang Huang
No abstract text is available yet for this article.
October 3, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28924445/the-first-argentinian-family-with-familial-amyloidosis-of-the-finnish-type
#20
Francisco Lucero Saá, Federico Andrés Cremona, Natalia Ximena Mínguez, María Laura Igarzabal, Pablo Chiaradía
Familial amyloidosis of the Finnish type or Meretoja syndrome is a rare autosomic dominant inherited systemic condition. It was first described by Meretoja in Finland in 1969. It is a disease produced by a single mutation in the gene coding for gelsolin, which generates an abnormal protein that cumulates in tissues and leads to various signs. Obtaining an early diagnosis can be challenging, as the first manifestations of the disease are ophthalmological and may only be seen with slit-lamp biomicroscopy. We present the first 3 cases diagnosed in Argentina, confirmed by genetic molecular testing...
May 2017: Case Reports in Ophthalmology
keyword
keyword
2288
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"