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Carbonic anhydrase 3

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https://www.readbyqxmd.com/read/28637277/measuring-co2-and-hco3-permeabilities-of-isolated-chloroplasts-using-a-mims-18o-approach
#1
Dimitri Tolleter, Vincent Chochois, Richard Poiré, G Dean Price, Murray R Badger
To support photosynthetic CO2 fixation by Rubisco, the chloroplast must be fed with inorganic carbon in the form of CO2 or bicarbonate. However, the mechanisms allowing the rapid passage of this gas and this charged molecule through the bounding membranes of the chloroplast envelope are not yet completely elucidated. We describe here a method allowing us to measure the permeability of these two molecules through the chloroplast envelope using a membrane inlet mass spectrometer and 18O-labelled inorganic carbon...
June 16, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28625714/proteomic-analysis-of-seminal-plasma-from-locally-adapted-curraleiro-p%C3%A3-duro-bulls-bos-taurus-identifying-biomarkers-involved-in-sperm-physiology-in-endangered-animals-for-conservation-of-biodiversity
#2
E B Menezes, R V de Oliveira, M F van Tilburg, E A Barbosa, N V Nascimento, A L M C S Velho, F B Moreno, R A Moreira, A C O Monteiro-Moreira, G M C Carvalho, A F Ramos, E Memili, A A Moura
The present study was aimed at evaluating the seminal plasma proteins and sperm parameters of Curraleiro Pé-Duro bulls. Semen was collected from 10 bulls by electroejaculation, and sperm parameters were evaluated in fresh and frozen-thawed semen. Seminal plasma proteins were analyzed by 2-D SDS-PAGE and mass spectrophotometry. Tools in computational biology were used to generate bioinformatic knowledge and evaluate gene ontology, protein-protein interactions, phylogenetic trees and multiple sequence alignments...
May 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28613396/evaluation-of-acetylcholinesterase-and-carbonic-anhydrase-inhibition-profiles-of-1-2-3-4-6-pentasubstituted-4-hydroxy-cyclohexanes
#3
Umit M Kocyigit, Parham Taslimi, Hayreddin Gezegen, İlhami Gulçin, Mustafa Ceylan
Carbonic anhydrase (CA; EC 4.2.1.1) is used for remedial purposes for several years, as there is significant focus on expanding more new activators (CAAs) and high affinity inhibitors. Alzheimer's disease and other similar ailments such as dementia and vascular dementia with Lewy bodies reduce cholinergic activity in the important areas involved in cognition and memory. Prevalent drugs for the symptomatic therapy of dementia are significant in increasing the associated cholinergic deficiency by inhibiting acetylcholinesterase (AChE)...
June 14, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28596233/adaptable-interaction-between-aquaporin-1-and-band-3-reveals-a-potential-role-of-water-channel-in-blood-co2-transport
#4
Kate Hsu, Ting-Ying Lee, Ammasi Periasamy, Fu-Jen Kao, Li-Tzu Li, Chuang-Yu Lin, Hui-Ju Lin, Marie Lin
Human CO2 respiration requires rapid conversion between CO2 and HCO3(-) Carbonic anhydrase II facilitates this reversible reaction inside red blood cells, and band 3 [anion exchange (AE)-1] provides a passage for HCO3(-) flux across the cell membrane. These 2 proteins are core components of the CO2 transport metabolon. Intracellular H2O is necessary for CO2/HCO3(-) conversion. However, abundantly expressed aquaporin (AQP)-1 in erythrocytes is thought not to be part of band 3 complexes or the CO2 transport metabolon...
June 8, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28557604/dapsone-hydroxylamine-mediated-alterations-in-human-red-blood-cells-from-endometriotic-patients
#5
Alessandra Andrisani, Gabriella Donà, Chiara Sabbadin, Stefano Dall'Acqua, Elena Tibaldi, Antonella Roveri, Valentina Bosello Travain, Anna Maria Brunati, Guido Ambrosini, Eugenio Ragazzi, Decio Armanini, Luciana Bordin
Endometriosis, an estrogen-dependent chronic gynecological disease in women of reproductive age, is characterized by a systemic inflammation status involving also red blood cells (RBCs). In this study, we evaluated how the protein oxidative status could be involved in the worsening of RBC conditions due to dapsone intake in endometriotic women in potential treatment for skin or infection diseases. Blood samples from two groups of volunteers, control group (CG) and endometriosis patient group (PG), were analyzed for their content of band 3 tyrosine phosphorylation (Tyr-P) and high molecular weight aggregate (HMWA) in membranes, and glutathione (GSH) content and carbonic anhydrase (CA) activity in cytosol...
May 30, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28553549/twenty-four-hour-variation-of-intraocular-pressure-in-primary-open-angle-glaucoma-treated-with-triple-eye-drops
#6
Yoshinori Itoh, Kenji Nakamoto, Hiroshi Horiguchi, Shumpei Ogawa, Takahiko Noro, Makoto Sato, Tadashi Nakano, Hiroshi Tsuneoka, Noriko Yasuda
Objectives. To evaluate 24-hour intraocular pressure (IOP) variation in patients with primary open-angle glaucoma (POAG) treated with triple eye drops. Subjects and Methods. The IOP was measured in 74 eyes in 74 POAG patients (seated) on triple therapy (PG analogue, β-blocker, carbonic anhydrase inhibitor) at about every 3 hours. Results. The peak IOP was 13.5 ± 3.1 at 1:00, and the trough IOP was at 12.6 ± 2.4 mmHg at 7:00. The IOP at 7:00 was significantly lower than that at 10:00, 1:00, and 3:00 (p < 0...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28553510/model-free-extraction-of-spin-label-position-distributions-from-pseudocontact-shift-data
#7
Elizaveta A Suturina, Daniel Häussinger, Kaspar Zimmermann, Luca Garbuio, Maxim Yulikov, Gunnar Jeschke, Ilya Kuprov
A significant problem with paramagnetic tags attached to proteins and nucleic acids is their conformational mobility. Each tag is statistically distributed within a volume between 5 and 10 Angstroms across; structural biology conclusions from NMR and EPR work are necessarily diluted by this uncertainty. The problem is solved in electron spin resonance, but remains open in the other major branch of paramagnetic resonance - pseudocontact shift (PCS) NMR spectroscopy, where structural biologists have so far been reluctantly using the point paramagnetic centre approximation...
April 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28524139/-ocular-hypotensive-effect-of-systemic-beta-blockers-in-patients-with-primary-glaucoma-and-arterial-hypertension
#8
A L Onishchenko, A V Kolbasko, A V Zakharova, E G Onishchenko, N M Zhilina
AIM: to evaluate the ocular hypotensive efficacy of systemic beta-blockers in primary glaucoma patients suffering from arterial hypertension (AH). MATERIAL AND METHODS: The study included 29 patients with POAG (58 eyes) aged from 47 to 83 years. Patients with stage I-III POAG received instillations of prostaglandin analogs and carbonic anhydrase inhibitors. All POAG patients also suffered from arterial hypertension and were prescribed selective beta-blockers (metoprolol, bisoprolol, or nebivalol) as monotherapy or as part of combination therapy (if the target arterial pressure had not been achieved under the initial treatment)...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/28524136/-clinical-evaluation-of-the-effectiveness-of-cholinesterase-inhibition-with-neuromidin-in-the-treatment-of-primary-glaucoma-patients
#9
I A Zakharova, R V Avdeev, V A Pristavka, S N Surnin, V Yu Makhmutov, I I Savrasova
AIM: to investigate neuromidin effectiveness in the treatment of patients with primary glaucoma and compensated intraocular pressure (IOP). MATERIAL AND METHODS: A total of 40 patients (80 eyes) were examined. Of them, 10 eyes with early glaucoma, 36 eyes with moderate-stage glaucoma, 33 eyes with advanced glaucoma, and 1 eye with end-stage glaucoma. In 19 eyes, IOP was controlled through beta-blockers, in 11 eyes - through carbonic anhydrase inhibitors, in 10 eyes - through prostaglandin analogues, and in 39 eyes - through combination drugs...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/28511907/potential-inhibitors-of-human-carbonic-anhydrase-isozymes-i-and-ii-design-synthesis-and-docking-studies-of-new-1-3-4-thiadiazole-derivatives
#10
Mehlika Dilek Altıntop, Belgin Sever, Ahmet Özdemir, Kaan Kucukoglu, Hicran Onem, Hayrunnisa Nadaroglu, Zafer Asım Kaplancıklı
In the last years, inhibition of carbonic anhydrase (CA) has emerged as a promising approach for pharmacologic intervention in a variety of disorders such as glaucoma, epilepsy, obesity, and cancer. As a consequence, the design of CA inhibitors (CAIs) is a highly dynamic field of medicinal chemistry. Due to the therapeutic potential of thiadiazoles as CAIs, new 1,3,4-thiadiazole derivatives were synthesized and investigated for their inhibitory effects on hCA I and hCA II. Although the tested compounds did not carry a sulfonamide group, an important pharmacophore for CA inhibitory activity, it was a remarkable finding that most of them were more effective on hCAs than acetazolamide (AAZ), the reference agent...
July 1, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28498313/identifying-the-genes-regulated-by-atwrky6-using-comparative-transcript-and-proteomic-analysis-under-phosphorus-deficiency
#11
Li-Qin Li, Lu-Ping Huang, Gang Pan, Lun Liu, Xi-Yao Wang, Li-Ming Lu
Phosphorus (P) is an important mineral nutrient for plant growth and development. Overexpressing AtWRKY6 (35S:WRKY6-9) was more sensitive and wrky6 (wrky6-1) was more resistant under low Pi conditions. To better understand the function of AtWRKY6 under low phosphate stress conditions, we applied two-dimensional gel electrophoresis (2-DE) to analyse differentially expressed proteins in the shoots and roots between wild type, 35S:WRKY6-9 and wrky6-1 after phosphorus deficiency treatment for three days. The results showed 88 differentially abundant protein spots, which were identified between the shoots and roots of 35S:WRKY6-9 and wrky6-1 plants...
May 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28497265/proteomics-analysis-of-pleomorphic-adenoma-of-the-human-parotid-gland
#12
Ahmet Mutlu, Murat Ozturk, Gurler Akpinar, Murat Kasap, Aylin Kanli
The objective of this study is to perform proteomic analysis of pleomorphic adenoma (PA) in the human parotid gland (PG) with comparison of normal PG. This is an individual prospective randomized controlled trial. This study was performed in a tertiary referral center. Tissue samples of PG and PA were taken after surgical excision of PG from 13 patients. Protein extracts were prepared and protein pools created from the soluble extracts were subjected to 2D-DIGE analysis. Proteins displaying regulation in their abundance were determined and identified using MALDIT-OF/TOF analysis...
May 11, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28496310/patient-satisfaction-with-fixed-combination-bimatoprost-timolol-ophthalmic-solution-a-survey-study-in-patients-with-glaucoma-in-china
#13
Xinghuai Sun, Mingkai Lin, Xuanchu Duan, Chun Zhang, Jian Ming
BACKGROUND: Poor adherence to treatment is a problem in glaucoma, and patient dissatisfaction with topical glaucoma medication is a barrier to adherence. The objective of this study was to evaluate glaucoma patients' satisfaction with fixed-combination bimatoprost/timolol ophthalmic solution (BTFC). METHODS: This observational, multicenter study was conducted in China in adults with glaucoma treated with BTFC for 1-3 months. Five hundred patients answered a questionnaire concerning their demographic characteristics, history of glaucoma and topical glaucoma treatment, and use of BTFC...
2017: Patient Preference and Adherence
https://www.readbyqxmd.com/read/28490422/cftr-mediated-anion-secretion-across-intestinal-epithelium-like-caco-2-monolayer-under-pth-stimulation-is-dependent-on-intermediate-conductance-k-channels
#14
Walailak Jantarajit, Kornkamon Lertsuwan, Jarinthorn Teerapornpuntakit, Nateetip Krishnamra, Narattaphol Charoenphandhu
Parathyroid hormone (PTH), a pleiotropic hormone that maintains mineral homeostasis, is also essential for controlling pH balance and ion transport across renal and intestinal epithelia. Optimization of luminal pH is important for absorption of trace elements, e.g., calcium and phosphorus. We have previously demonstrated that PTH rapidly stimulated electrogenic HCO3(-) secretion in intestinal epithelial-like Caco-2 monolayer, but the underlying cellular mechanism, contributions of other ions, particularly Cl(-) and K(+), and long-lasting responses are not completely understood...
May 10, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28482217/ruthenium-ii-complexes-of-saccharin-with-dipyridoquinoxaline-and-dipyridophenazine-structures-biological-interactions-and-photoinduced-dna-damage-activity
#15
Priyaranjan Kumar, Srikanth Dasari, Ashis K Patra
Ruthenium complexes trans-[Ru(sac)2(dpq)2] (1) and trans-[Ru(sac)2(dppz)2] (2) where sac is artificial sweetener saccharin (o-sulfobenzimide; 1,2-benzothiazole-3(2H)-one1,1-dioxide (Hsac)), dpq = dipyrido[3,2-d:2',3'-f]quinoxaline and dppz = dipyrido[3,2-a:2',3'-c]phenazine have been synthesized and thoroughly characterized using various analytical and spectral techniques. Saccharin known to act as carbonic anhydrase IX (CA IX) inhibitor which is a biomarker for highly aggressive and proliferative tumor in hypoxic stress, so inhibition of CA IX is a potential strategy for anticancer chemotherapy...
May 3, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28473691/notochordal-and-nucleus-pulposus-marker-expression-is-maintained-by-sub-populations-of-adult-human-nucleus-pulposus-cells-through-aging-and-degeneration
#16
Stephen M Richardson, Francesca E Ludwinski, Kanna K Gnanalingham, Ross A Atkinson, Anthony J Freemont, Judith A Hoyland
The nucleus pulposus (NP) of the intervertebral disc (IVD) demonstrates substantial changes in cell and matrix composition with both ageing and degeneration. While recent transcriptomic profiling studies have helped define human NP cell phenotype, it remains unclear how expression of these markers is influenced by ageing or degeneration. Furthermore, cells of the NP are thought to derive from the notochord, although adult NP lacks identifiable notochordal (NC) cells. This study aimed to confirm expression of previously identified NP and NC marker genes in adult human NP cells from a range of ages and degenerate states...
May 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28467665/bryophyte-gas-exchange-dynamics-along-varying-hydration-status-reveal-a-significant-carbonyl-sulphide-cos-sink-in-the-dark-and-cos-source-in-the-light
#17
Teresa E Gimeno, Jérôme Ogée, Jessica Royles, Yves Gibon, Jason B West, Régis Burlett, Sam P Jones, Joana Sauze, Steven Wohl, Camille Benard, Bernard Genty, Lisa Wingate
Carbonyl sulphide (COS) is a potential tracer of gross primary productivity (GPP), assuming a unidirectional COS flux into the vegetation that scales with GPP. However, carbonic anhydrase (CA), the enzyme that hydrolyses COS, is expected to be light independent, and thus plants without stomata should continue to take up COS in the dark. We measured net CO2 (A(C) ) and COS (A(S) ) uptake rates from two astomatous bryophytes at different relative water contents (RWCs), COS concentrations, temperatures and light intensities...
May 3, 2017: New Phytologist
https://www.readbyqxmd.com/read/28453941/probing-molecular-interactions-between-human-carbonic-anhydrases-hcas-and-a-novel-class-of-benzenesulfonamides
#18
Elvira Bruno, Maria Rosa Buemi, Anna Di Fiore, Laura De Luca, Stefania Ferro, Andrea Angeli, Roberto Cirilli, Daniele Sadutto, Vincenzo Alterio, Simona Maria Monti, Claudiu T Supuran, Giuseppina De Simone, Rosaria Gitto
On the basis of X-ray crystallographic studies of the complex of hCA II with 4-(3,4-dihydro-1H-isoquinoline-2-carbonyl)benzenesulfonamide (3) (PDB code 4Z1J ), a novel series of 4-(1-aryl-3,4-dihydro-1H-isoquinolin-2-carbonyl)benzenesulfonamides (23-33) was designed. Specifically, our idea was to improve the selectivity toward druggable isoforms through the introduction of additional hydrophobic/hydrophilic functionalities. Among the synthesized and tested compounds, the (R,S)-4-(6,7-dihydroxy-1-phenyl-3,4-tetrahydroisoquinoline-1H-2-carbonyl)benzenesulfonamide (30) exhibited a remarkable inhibition for the brain-expressed hCA VII (Ki = 0...
May 25, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28445001/inhibition-properties-of-some-flavonoids-on-carbonic-anhydrase-i-and-ii-isoenzymes-purified-from-human-erythrocytes
#19
Zübeyir Huyut, Şükrü Beydemir, İlhami Gülçin
Carbonic anhydrases (CAs, E.C.4.2.1.1) play a critical role in many important physiological events and treatment of some diseases. Flavonoids or phenolic compounds have been discovered as novel CAs inhibitors instead of the traditional sulfonamides, with different binding to CAs, pro-drug activities, and new inhibition mechanisms. Here, we investigated the inhibition effects of some flavonoids including malvin, callistephin, oenin, pelargonin, silychristin, and 1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol (ID-8) against hCA I and II, which purified from human erythrocytes by affinity column chromatography...
April 26, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28416105/do-clear-cell-papillary-renal-cell-carcinomas-have-malignant-potential-diolombi-ml-cheng-l-argani-p-epstein-ji-am-j-surg-pathol-december-2015-39-12-1621-1634
#20
Oleksandr N Kryvenko
There have been no recurrences or metastases of clear cell papillary renal cell carcinoma (CCPRCC) in 268 reported cases with follow-up in the English-language literature. We identified all our cases of CCPRCC (1990-2013), reviewing all cases that preceded the formal designation of the entity. Immunohistochemical stains were performed on 32 cases during their initial workup. In addition, stains for carbonic anhydrase IX and cytokeratin 7 were performed on 2 cases, one with atypical follow-up and the other with a more compact morphology, although not performed initially...
June 2017: Urologic Oncology
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