keyword
MENU ▼
Read by QxMD icon Read
search

Viral diarrhea

keyword
https://www.readbyqxmd.com/read/28717858/complete-nucleotide-sequence-of-a-novel-bovine-viral-diarrhea-virus-subtype-1-isolate-from-italy
#1
Moira Bazzucchi, Luigi Bertolotti, Letizia Ceglie, Monica Giammarioli, Elisabetta Rossi, Sergio Rosati, Gian Mario De Mia
To gain further insight into the genomic features of bovine viral diarrhea virus 1 (BVDV-1) subtypes, we sequenced the complete genome of the BVDV-1 isolate VE/245/12. This is an uncommon subtype that was isolated from a persistently infected animal. Here, we report the complete genome sequence, consisting of 12,295 nucleotides (nt) with an open reading frame of 11,694 nt encoding 3,898 amino acids. Phylogenetic analysis of the full-length genome, 5'-UTR, and N(pro) region confirmed that the BVDV-1 isolate differed significantly from all of the bovine pestiviruses identified so far, providing evidence for the presence of a distinct novel genetic group...
July 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28715653/inhibition-of-nf-%C3%AE%C2%BAb-activity-by-the-porcine-epidemic-diarrhea-virus-nonstructural-protein-1-for-innate-immune-evasion
#2
Qingzhan Zhang, Jinyou Ma, Dongwan Yoo
Porcine epidemic diarrhea virus emerged in the US is known to suppress the type I interferons response during infection. In the present study using porcine epithelial cells, we showed that PEDV inhibited both NF-κB and proinflammatory cytokines. PEDV blocked the p65 activation in infected cells and suppressed the PRD II-mediated NF-κB activity. Of the total of 22 viral proteins, nine proteins were identified as NF-κB antagonists, and nsp1 was the most potent suppressor of proinflammatory cytokines. Nsp1 interfered the phosphorylation and degradation of IκBα, and thus blocked the p65 activation...
July 14, 2017: Virology
https://www.readbyqxmd.com/read/28702931/autophagy-induced-by-bovine-viral-diarrhea-virus-infection-counteracts-apoptosis-and-innate-immune-activation
#3
Yulong Zhou, Yachao Ren, Yanlong Cong, Yu Mu, Renfu Yin, Zhuang Ding
Bovine viral diarrhea virus (BVDV) is an important pathogen of cattle that plays a complex role in disease. There are two biotypes of BVDV: non-cytopathic (NCP) and cytopathic (CP). One strategy that has been used to treat or prevent virus-associated diseases is the modulation of autophagy, which is used by the innate immune system to defend against viral infection; however, at present, the interplay between autophagy and BVDV remains unclear. Madin-Darby bovine kidney cells stably expressing microtubule-associated protein 1 light chain 3B (LC3B) with green fluorescent protein (GFP) (GFP-LC3-MDBK cells) and autophagy-deficient MDBKs (shBCN1-MDBK cells) were constructed...
July 12, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28702050/circulating-micrornas-in-serum-from-cattle-challenged-with-bovine-viral-diarrhea-virus
#4
Tasia M Taxis, Fernando V Bauermann, Julia F Ridpath, Eduardo Casas
Bovine viral diarrhea virus (BVDV) is an RNA virus that is often associated with respiratory disease in cattle. MicroRNAs have been proposed as indicators of exposure to respiratory pathogens. The objective of this study was to identify microRNAs in cattle that had been challenged with a non-cytopathic field strain of BVDV. Five colostrum deprived neonate Holstein calves were inoculated with BVDV (challenged) and 4 were mock challenged (control). Serum from all calves was collected at four different times: prior to challenge (day 0) and at 4, 9, and 16 days post-challenge...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28693308/isolation-and-characterization-of-a-new-porcine-epidemic-diarrhea-virus-variant-that-occurred-in-korea-in-2014
#5
Dong-Kun Yang, Ha-Hyun Kim, Seong-Hun Lee, Soon-Seek Yoon, Joong-Won Park, In-Soo Cho
Porcine epidemic diarrhea (PED) has caused significant economic losses in the swine industry, and another epidemic occurred recently in Korea in 2014. Isolating and culturing the PED virus (PEDV) allow investigations into its pathogenesis and development of vaccines and diagnostic assays. In this study, we successfully isolated two PEDV isolates (QIAP1401 and QIAP1402) from naturally infected piglets in Jeju-do. Viral propagation was confirmed in Vero cells according to cytopathic effects, immunofluorescence assay, reverse transcription-polymerase chain reaction and electron microscopic analyses...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28688828/epidemiology-clinical-features-and-microbiology-of-patients-with-diarrhea-in-community-clinics-in-taiwan
#6
Chih-Yu Chi, Li-Na Liao, Cheng-Mao Ho, Chia-Huei Chou, Mao-Wang Ho, Jen-Hsein Wang
OBJECTIVE: To investigate the clinical features and microbiology of patients with diarrheal diseases in Taiwan. METHODS: From March 2014 to October 2014, patients with diarrheal diseases referred from the community clinics were enrolled into our prospective study. Demographics and clinical features of the participants were acquired. Stool samples were examined by the Luminex Gastrointestinal Pathogen Panel assay. Data were analyzed by SAS version 9.4. RESULTS: A total of 545 patients were enrolled into this study...
June 28, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28688346/improved-detection-of-bovine-viral-diarrhea-virus-in-bovine-lymphoid-cell-lines-using-primeflow-rna-assay
#7
Shollie M Falkenberg, Rohana P Dassanayake, John D Neill, Julia F Ridpath
Bovine viral diarrhea virus (BVDV) infections, whether as acute, persistent or contributing to co-infections, result in significant losses for cattle producers. Although, BVDV can be identified readily by real-time PCR and ELISA, detection and quantification of viral infection at the single cell level is extremely difficult. Detection at the single lymphoid cell level is important due to the immunomodulation that accompanies BVDV infection. A novel PrimeFlow RNA assay using in-situ detection of BVDV was evaluated...
September 2017: Virology
https://www.readbyqxmd.com/read/28686540/replicon-particle-expressing-the-e2-glycoprotein-of-bovine-viral-diarrhea-virus-immunization-and-evaluation-of-antibody-response
#8
Mohammad M Hossain, Raymond R Rowland
The objective of this study was to develop a new antigen delivery system using an alphavirus replicon particle (RP) to induce humoral antibody responses against bovine viral diarrhea virus (BVDV) recombinant antigen produced from envelope glycoprotein E2. An alphavirus RP expressing the E2 glycoprotein of BVDV was used for immunization of pigs. A fluorescent microsphere immunoassay (FMIA) has been applied to detect BVDV E2 antigen-specific antibody isotype in pig immunized with alphavirus RP. Full-length BVDV E2 (aa 1-375) was cleaved into several pieces, eight E2 DNA fragments, including full-length DNA, were cloned into expression vector pHUE, and the recombinant proteins expressed in BL-21 (DE3) Escherichia coli...
July 7, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28684708/porcine-epidemic-diarrhea-in-europe-in-detail-analyses-of-disease-dynamics-and-molecular-epidemiology
#9
Dennis Hanke, Anne Pohlmann, Carola Sauter-Louis, Dirk Höper, Julia Stadler, Mathias Ritzmann, Adi Steinrigl, Bernd-Andreas Schwarz, Valerij Akimkin, Robert Fux, Sandra Blome, Martin Beer
Porcine epidemic diarrhea (PED) is an acute and highly contagious enteric disease of swine caused by the eponymous virus (PEDV) which belongs to the genus Alphacoronavirus within the Coronaviridae virus family. Following the disastrous outbreaks in Asia and the United States, PEDV has been detected also in Europe. In order to better understand the overall situation, the molecular epidemiology, and factors that might influence the most variable disease impact; 40 samples from swine feces were collected from different PED outbreaks in Germany and other European countries and sequenced by shot-gun next-generation sequencing...
July 6, 2017: Viruses
https://www.readbyqxmd.com/read/28677409/serologic-evidence-of-hobi-like-virus-circulation-in-argentinean-water-buffalo
#10
Andrea Pecora, María S Pérez Aguirreburualde, Dario A Malacari, Osvaldo Zabal, Juan M Sala, José L Konrad, Sergio G Caspe, Fernando Bauermann, Julia Ridpath, María J Dus Santos
HoBi-like pestiviruses (also known as bovine viral diarrhea virus 3) have been sporadically reported from naturally infected cattle in Brazil, Asia, and Europe. Although HoBi-like viruses seem to be endemic in Brazilian cattle and buffalo, they have not been studied in the other countries of South America to our knowledge. Herein we report serologic results of buffalo from 12 large farms in Argentina located near the Brazilian border. These buffalo were not vaccinated against pestiviruses. Our results indicate that HoBi-like virus may be circulating in the northeastern region of Argentina given that half of the analyzed animals showed high levels of neutralizing antibodies against the pestivirus...
July 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28669036/genomic-and-antigenic-relationships-between-two-hobi-like-strains-and-other-members-of-the-pestivirus-genus
#11
Ana Cristina S Mósena, Samuel P Cibulski, Matheus N Weber, Simone Silveira, Mariana S Silva, Fabiana Q Mayer, Paulo M Roehe, Cláudio W Canal
'HoBi'-like viruses comprise a putative new species within the genus Pestivirus of the family Flaviviridae. 'HoBi'-like viruses have been detected worldwide in batches of fetal calf serum, in surveillance programs for bovine pestiviruses and from animals presenting clinical signs resembling bovine viral diarrhea virus (BVDV)-associated diseases. To date, few complete genome sequences of 'HoBi'-like viruses are available in public databases. Moreover, detailed analyses of such genomes are still scarce. In an attempt to expand data on the genetic diversity and biology of pestiviruses, two genomes of 'HoBi'-like viruses recovered from Brazilian cattle were described and characterized in this study...
July 1, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28668732/evolution-of-bovine-viral-diarrhea-virus-in-canada-from-1997-to-2013
#12
Adam Chernick, Frank van der Meer
Bovine viral diarrhea virus (BVDV) is a rapidly evolving, single-stranded RNA virus and a production limiting pathogen of cattle worldwide. 79 viral isolates collected between 1997 and 2013 in Canada were subjected to next-generation sequencing. Bayesian phylogenetics was used to assess the evolution of this virus. A mean substitution rate of 1.4×10(-3) substitutions/site/year was found across both BVDV1 and BVDV2. Evolutionary rates in the E2 gene were slightly faster than other regions. We also identified population structures below the sub-genotype level that likely have phenotypic implications...
September 2017: Virology
https://www.readbyqxmd.com/read/28662668/experimentally-confirmed-toltrazuril-resistance-in-a-field-isolate-of-cystoisospora-suis
#13
Aruna Shrestha, Barbara Freudenschuss, Rutger Jansen, Barbara Hinney, Bärbel Ruttkowski, Anja Joachim
BACKGROUND: Constant treatment regimens with toltrazuril against Cystoisospora suis infection in piglets are being applied in the intensive production systems for the last two decades, but the possibility of resistance development has not been addressed so far despite limited availability of treatment alternatives. Recently, a pig producer in The Netherlands who routinely used toltrazuril complained about diarrhea in suckling piglets in the absence of bacterial and viral pathogens, and oocysts of C...
June 29, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28662012/fatal-course-of-takotsubo-cardiomyopathy-in-a-female-with-recurrent-clostridium-difficile-infection
#14
Waldemar Elikowski, Małgorzata Małek-Elikowska, Monika Lisiecka, Iwona Mozer-Lisewska
Among diverse triggering factors of stress-induced takotsubo cardiomyopathy (TC), a viral or bacterial infection is rarely observed. Sepsis is an exception, regardless of the etiologic pathogen, in which case an excess of catecholamines may result in acute left ventricular dysfunction. TC precipitated by Clostridium difficile infection (CDI) has been reported only in two patients so far. A CASE REPORT: The authors describe another case of TC triggered this time by recurrent C. difficile colitis which occurred in a 72-yearold female...
June 23, 2017: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28659484/case-control-comparison-of-enteric-viromes-in-captive-rhesus-macaques-with-acute-or-idiopathic-chronic-diarrhea
#15
Beatrix Kapusinszky, Amir Ardeshir, Usha Mulvaney, Xutao Deng, Eric Delwart
Diarrhea is the major cause of non-research associated morbidity and mortality affecting the supply of rhesus macaques and, potentially, their responses to experimental treatments. Idiopathic chronic diarrhea (ICD) in rhesus macaques also resembles ulcerative colitis, a form of human inflammatory bowel disease. To test for viral etiologies we characterized and compared the fecal virome from 32 healthy, 31 with acute diarrhea, and 29 with ICD animals. The overall fraction of eukaryotic viral reads was 0.063% for the healthy group, 0...
June 28, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28655215/development-of-a-new-method-based-on-unmodified-gold-nanoparticles-and-peptide-nucleic-acids-for-detecting-bovine-viral-diarrhea-virus-rna
#16
Maryam Askaravi, Seyedeh Elham Rezatofighi, Saadat Rastegarzadeh, Masoud Reza Seifi Abad Shapouri
A simple colorimetric assay is presented for detecting bovine viral diarrhea virus (BVDV)-RNA based on aggregation of gold nanoparticles (AuNPs) in the presence of charge-neutral peptide nucleic acids (PNA). Free charge-neutral PNA oligomers tended to be adsorbed onto AuNPs and act as a coagulant, whereas hybridizing complementary RNA with PNA disrupted PNA-induced AuNP aggregation, and the NPs remained stable. However, non-complementary RNA did not have this effect, and PNA induced aggregation of the AuNPs that resulted in a color change of the reaction from red to blue...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28653489/household-sanitation-is-associated-with-lower-risk-of-bacterial-and-protozoal-enteric-infections-but-not-viral-infections-and-diarrhea-in-a-cohort-study-in-a-low-income-urban-neighborhood-in-vellore-india
#17
David Berendes, Juan Leon, Amy Kirby, Julie Clennon, Suraja Raj, Habib Yakubu, Katharine Robb, Arun Kartikeyan, Priya Hemavathy, Annai Gunasekaran, Sheela Roy, Ben Chirag Ghale, J Senthil Kumar, Venkata Raghava Mohan, Gagandeep Kang, Christine Moe
OBJECTIVE: This study examined associations between household sanitation and enteric infection-including diarrheal-specific outcomes-in children 0-2 years of age in a low-income, dense urban neighborhood. METHODS: As part of the MAL-ED study, 230 children in a low-income, urban, Indian neighborhood provided stool specimens at 14-17 scheduled time points and during diarrheal episodes in the first two years of life that were analyzed for bacterial, parasitic (protozoa and helminths), and viral pathogens...
June 27, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28637760/discovery-of-a-highly-divergent-coronavirus-in-the-asian-house-shrew-from-china-illuminates-the-origin-of-the-alphacoronaviruses
#18
Wen Wang, Xian-Dan Lin, Yong Liao, Xiao-Qing Guan, Wen-Ping Guo, Jian-Guang Xing, Edward C Holmes, Yong-Zhen Zhang
Although shrews are one of the largest groups of mammals little is known about their role in the evolution and transmission of viral pathogens including coronaviruses. We captured 266 Asian house shrews (Suncus murinus) in Jiangxi and Zhejiang provinces, China, during 2013-2015. Coronavirus (CoV) RNA was detected in 24 Asian house shrews, with an overall prevalence of 9.02%. Complete viral genome sequences were successfully recovered from the RNA positive samples. The newly discovered shrew CoV fell into four lineages reflecting their geographic origins, indicative of largely allopatric evolution...
June 21, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28634691/modulation-of-rotavirus-severe-gastroenteritis-by-the-combination-of-probiotics-and-prebiotics
#19
REVIEW
Guadalupe Gonzalez-Ochoa, Lilian K Flores-Mendoza, Ramona Icedo-Garcia, Ricardo Gomez-Flores, Patricia Tamez-Guerra
Annual mortality rates due to infectious diarrhea are about 2.2 million; children are the most vulnerable age group to severe gastroenteritis, representing group A rotaviruses as the main cause of disease. One of the main factors of rotavirus pathogenesis is the NSP4 protein, which has been characterized as a viral toxin involved in triggering several cellular responses leading to diarrhea. Furthermore, the rotavirus protein NSP1 has been associated with interferon production inhibition by inducing the degradation of interferon regulatory factors IRF3, IRF5, and IRF7...
June 20, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28622871/a-spike-specific-whole-porcine-antibody-isolated-from-a-porcine-b-cell-that-neutralizes-both-genogroup-1-and-2-pedv-strains
#20
Fang Fu, Lin Li, Lingling Shan, Beibei Yang, Hongyan Shi, Jiaoer Zhang, Hongfeng Wang, Li Feng, Pinghuang Liu
Porcine epidemic diarrhea (PED), caused by an alpha coronavirus, is a highly contagious disease and causes high morbidity and mortality in suckling piglets. Isolating PEDV neutralizing antibodies from porcine B cells is critical to elucidate the development of PEDV neutralizing antibodies and the protective mechanism of PEDV infection. Here, we described the isolation of a PEDV-neutralizing antibody from the B cell of a vaccinated pig. The antibody, named PC10, was demonstrated to target the conformational epitope of PEDV spike protein, specifically bind to the infected cells of PEDV genogroup 1 and 2 strains, and potently neutralize PEDV infection...
June 2017: Veterinary Microbiology
keyword
keyword
22815
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"