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https://www.readbyqxmd.com/read/29160519/ex-vivo-t-lymphocyte-chemokine-receptor-phenotypes-in-patients-with-chronic-chagas-disease
#1
Matheus Barbosa de Miranda, Adriene Siqueira de Melo, Mariana Silva Almeida, Silvia Martins Marinho, Wilson Oliveira, Yara de Miranda Gomes
INTRODUCTION: Elucidating the molecules involved in the inflammatory process of chronic Chagas disease may allow identification of treatment targets. METHODS: The ex vivo phenotypic expression of chemokine receptors CCR1, CCR3, CCR4, CCR5, CXCR2, CXCR3, CXCR4, and CXCR5 on the CD4+ and CD8+ T-cells of patients with chronic Chagas cardiomyopathy of varying severity was evaluated using flow cytometry. RESULTS: Differential expression of CD4+CCR3+ and CD8+CCR4+ T-cells was observed in patients with mild cardiac involvement compared, respectively, with patients with severe cardiac and asymptomatic forms of Chagas disease...
September 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29132135/increased-th1-th17-responses-contribute-to-low-grade-inflammation-in-age-related-macular-degeneration
#2
Jiajia Chen, Wenzhan Wang, Qiuming Li
BACKGROUND/AIMS: Age-related macular degeneration (AMD) is the primary cause of senior blindness in developed countries. Mechanisms underlying initiation and development of AMD remained known. METHODS: We examined the CD4+ T cell compartments and their functions in AMD patients. RESULTS: AMD patients presented significantly higher frequencies of interferon (IFN)-γ-expressing and interleukin (IL)-17-expressing CD4+ T cells than healthy controls...
November 13, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29121993/transcriptomic-analyses-reveal-the-underlying-pro-malignant-functions-of-pthr1-for-osteosarcoma-via-activation-of-wnt-and-angiogenesis-pathways
#3
Shenglong Li, Yujin Dong, Ke Wang, Zhe Wang, Xiaojing Zhang
BACKGROUND: Increasing evidence has indicated parathyroid hormone type 1 receptor (PTHR1) plays important roles for the development and progression of osteosarcoma (OS). However, its function mechanisms remain unclear. The goal of this study was to further illuminate the roles of PTHR1 in OS using microarray data. METHODS: Microarray data were available from the Gene Expression Omnibus database under the accession number GSE46861, including six tumors from mice with PTHR1 knockdown (PTHR1...
November 9, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29089446/mir-505-3p-controls-chemokine-receptor-up-regulation-in-macrophages-role-in-familial-hypercholesterolemia
#4
Rafael Escate, Pedro Mata, Jose Maria Cepeda, Teresa Padró, Lina Badimon
Familial hypercholesterolemia (FH) conveys a high risk of premature atherosclerosis as a result of lifelong exposure to high LDL cholesterol levels that are not fully reduced by standard-of-care lipid-lowering treatment. Inflammatory mediators have played a role in the progression of atherosclerotic lesions. Here, we investigated whether innate immunity cells in patients with FH have a specific proinflammatory phenotype that is distinct from that of cells in normal participants. To this end, miR-505-3p-a microRNA related to chronic inflammation-and its target genes were investigated in monocyte-derived macrophages (MACs) of patients with FH (FH-MACs) and non-FH controls (co-MACs)...
October 31, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29062168/illicium-verum-extract-and-trans-anethole-attenuate-ovalbumin-induced-airway-inflammation-via-enhancement-of-foxp3-regulatory-t-cells-and-inhibition-of-th2-cytokines-in-mice
#5
Yoon-Young Sung, Seung-Hyung Kim, Dong-Seon Kim, Ji-Eun Lee, Ho Kyoung Kim
Illicium verum is used in traditional medicine to treat inflammation. The study investigates the effects of IVE and its component, trans-anethole (AET), on airway inflammation in ovalbumin- (OVA-) induced asthmatic mice. Asthma was induced in BALB/c mice by systemic sensitization to OVA, followed by intratracheal, intraperitoneal, and aerosol allergen challenges. IVE and AET were orally administered for four weeks. We investigated the effects of treatment on airway hyperresponsiveness, IgE production, pulmonary eosinophilic infiltration, immune cell phenotypes, Th2 cytokine production in bronchoalveolar lavage, Th1/Th2 cytokine production in splenocytes, forkhead box protein 3 (Foxp3) expression, and lung histology...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/29051319/ccl26-participates-in-the-prl-3-induced-promotion-of-colorectal-cancer-invasion-by-stimulating-tumor-associated-macrophage-infiltration
#6
Qiusheng Lan, Wei Lai, Yujie Zeng, Lu Liu, Shoufeng Li, Shaowen Jin, Yang Zhang, Xingxi Luo, Heyang Xu, Xiangan Lin, Zhonghua Chu
Both phosphatase of regenerating liver-3 (PRL-3) and tumor-associated macrophages (TAMs) influence cancer progression. Whether PRL-3 plays a critical role in colorectal cancer (CRC) invasion and metastasis by inducing TAM infiltration remains unclear. In the present study, we investigated the effects of chemokine ligand 26 (CCL26) on TAM infiltration and CRC invasion and the underlying mechanism in CRC cells by overexpressing or silencing PRL-3. We found that PRL-3 up-regulated CCL26 expression correlatively and participated in cell migration, according to the results of gene ontology analysis...
October 19, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29045346/autoimmune-hepatitis-and-aih-overlap-with-sclerosing-cholangitis-immunophenotype-markers-in-children-and-adolescents
#7
Priscila Menezes Ferri, Ana Cristina Simões E Silva, Karen Cecília de Lima Torres, Soraya Luiza Campos Silva, Diego Júnior Queiroga de Aquino, Maria Luísa Marques Ferreira, Eleonora Druve Tavares Fagundes, Débora Marques de Miranda, Alexandre Rodrigues Ferreira
OBJECTIVE: The pathophysiology of autoimmune hepatitis (AIH) may involve the activation of immune cells and changes in the expression of cellular markers. The aim of this study was to characterize the immunophenotype markers of lymphocytes and monocytes in the peripheral blood of children and adolescents with type 1 AIH and AIH overlap with sclerosing cholangitis (overlap syndrome, OS). METHODS: This is a cross-sectional study of 20 children and adolescents diagnosed with type 1 AIH and 19 with OS...
October 17, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29042950/expression-of-ccr3-sox5-and-lc3-in-patients-with-elderly-onset-rheumatoid-arthritis-and-the-clinical-significance
#8
Jing Wang, Qing Zhao
This study investigated the expression of C-C chemokine receptor type 3 (CCR3), transcription factor SOX5 (SOX5) and microtubule-associated protein 1 light chain 3 (LC3) in patients with elderly onset rheumatoid arthritis (EORA) and the clinical significance. Ninety patients with elderly onset rheumatoid arthritis were selected in our hospital from January to December in 2016 to serve as patient group. At the same time, 50 healthy people were selected as control group. Levels of CCR3, SOX5 and LC3 in serum of two groups were detected by enzyme-linked immunosorbent assay (ELISA)...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29029332/identification-of-a-novel-subset-of-myeloid-derived-suppressor-cells-during-chronic-staphylococcal-infection-that-resembles-immature-eosinophils
#9
Oliver Goldmann, Andreas Beineke, Eva Medina
We have previously reported that myeloid-derived suppressor cells (MDSC), which are a heterogeneous population of immunosuppressive immature myeloid cells, expanded during chronic Staphylococcus aureus infection and promoted bacterial persistence by inhibiting effector T cells. Two major MDSC subsets including monocytic MDSCs (M-MDSC) and granulocytic MDSCs (G-MDSC) have been described to date. Here, we identified a new subset of MDSC (Eo-MDSC) in S. aureus-infected mice that phenotypically resembles eosinophils...
September 23, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28994588/regulation-of-the-oligomeric-status-of-ccr3-with-binding-ligands-revealed-by-single-molecule-fluorescence-imaging
#10
Yanzhuo Song, Baosheng Ge, Jun Lao, Zhencai Wang, Bin Yang, Xiaojuan Wang, Hua He, Jiqiang Li, Fang Huang
The relationship between the oligomeric status and functions of chemokine receptor CCR3 is still controversial. We use total internal reflection fluorescence microscopy at the single-molecule level to visualize the oligomeric status of CCR3 and its regulation of the membrane of stably transfected T-REx-293 cells. We find that the population of the dimers and oligomers of CCR3 can be modulated by the binding of ligands. Natural agonists can induce an increase in the level of dimers and oligomers at high concentrations, whereas antagonists do not have a significant influence on the oligomeric status...
October 30, 2017: Biochemistry
https://www.readbyqxmd.com/read/28986277/upregulation-of-peripheral-cxc-and-cc-chemokine-receptor-expression-on-cd4-t-cells-is-associated-with-immune-dysregulation-in-children-with-autism
#11
Sheikh F Ahmad, Mushtaq A Ansari, Ahmed Nadeem, Saleh A Bakheet, Laila Yousef Al-Ayadhi, Sabry M Attia
Autism spectrum disorders (ASD) are characterized by disturbances in social interactions and communication, restricted repetitive interests, and stereotyped behavior. Cumulative evidence recommends that there are immune alterations in ASD. Chemokine receptors are known to play an important role in the central nervous system (CNS) and in many neuro inflammatory disorders. The main objective of this study was to explore the role of CXC and CC chemokine receptors signaling in children with autism. We examined chemokine receptor production of CXCR2, CXCR3, CXCR5, and CXCR7 in all peripheral blood mononuclear cells (PBMCs) and in CD4(+) T cells of typically developing control children (TD) and autistic children (AU)...
October 3, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28936366/constitutive-and-stress-induced-expression-of-ccl5-machinery-in-rodent-retina
#12
D'Anne S Duncan, William M McLaughlin, Noah Vasilakes, Franklin D Echevarria, Cathryn R Formichella, Rebecca M Sappington
Signaling by inflammatory cytokines and chemokines is associated with neurodegeneration in disease and injury. Here we examined expression of the β-chemokine CCL5 and its receptors in the mouse retina and evaluated its relevance in glaucoma, a common optic neuropathy associated with sensitivity to intraocular pressure (IOP). Using quantitative PCR, fluorescent in situ hybridization, immunohistochemistry and quantitative image analysis, we found CCL5 mRNA and protein was constitutively expressed in the inner retina and synaptic layers...
June 2017: Journal of Clinical & Cellular Immunology
https://www.readbyqxmd.com/read/28878766/chronic-exposure-to-malaria-is-associated-with-inhibitory-and-activation-markers-on-atypical-memory-b-cells-and-marginal-zone-like-b-cells
#13
Itziar Ubillos, Joseph J Campo, Pilar Requena, Maria Ome-Kaius, Sarah Hanieh, Honor Rose, Paula Samol, Diana Barrios, Alfons Jiménez, Azucena Bardají, Ivo Mueller, Clara Menéndez, Stephen Rogerson, Gemma Moncunill, Carlota Dobaño
In persistent infections that are accompanied by chronic immune activation, such as human immunodeficiency virus, hepatitis C virus, and malaria, there is an increased frequency of a phenotypically distinct subset of memory B cells lacking the classic memory marker CD27 and showing a reduced capacity to produce antibodies. However, critical knowledge gaps remain on specific B cell changes and immune adaptation in chronic infections. We hypothesized that expansion of atypical memory B cells (aMBCs) and reduction of activated peripheral marginal zone (MZ)-like B cells in constantly exposed individuals might be accompanied by phenotypic changes that would confer a tolerogenic profile, helping to establish tolerance to infections...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28843907/ccl28-chemokine-an-anchoring-point-bridging-innate-and-adaptive-immunity
#14
REVIEW
Teena Mohan, Lei Deng, Bao-Zhong Wang
Chemokines are an extensive family of small proteins which, in conjunction with their receptors, guide the chemotactic activity of various immune cells throughout the body. CCL28, β- or CC chemokine, is involved in the host immunity at various epithelial and mucosal linings. The unique roles of CCL28 in several facets of immune responses have attracted considerable attention and may represent a promising approach to combat various infections. CCL28 displays a broad spectrum of antimicrobial activity against gram-negative and gram-positive bacteria, as well as fungi...
August 24, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28826362/mir-3074-5p-promotes-the-apoptosis-but-inhibits-the-invasiveness-of-human-extravillous-trophoblast-derived-htr8-svneo-cells-in-vitro
#15
Yan Gu, Yan Shi, Qian Yang, Wen-Wen Gu, Ya-Ping He, Hua-Jun Zheng, Xuan Zhang, Jian-Mei Wang, Jian Wang
OBJECTIVE: The objective of this study was to observe the effects of the overexpression of miR-3074-5p in human trophoblast cells in vitro. DESIGN: Experimental in vitro study in HTR8/SVneo cells. METHODS: HTR8/SVneo cells were transfected with miR-3074-5p mimic. The cell apoptosis and invasion were measured via flow cytometry and transwell assay, respectively. The expression levels of P53, Cyclin Dependent Kinase Inhibitor 1B (P27), BCL-2, BCL2 associated X (BAX), and BCL2 like 14 (BCL-G) in HTR8/SVneo cells were determined by Western blot...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28706221/ccl11-a-novel-mediator-of-inflammatory-bone-resorption
#16
Elin Kindstedt, Cecilia Koskinen Holm, Rima Sulniute, Irene Martinez-Carrasco, Richard Lundmark, Pernilla Lundberg
Normal bone homeostasis, which is regulated by bone-resorbing osteoclasts and bone-forming osteoblasts is perturbed by inflammation. In chronic inflammatory disease with disturbed bone remodelling, e.g. rheumatoid arthritis, patients show increased serum levels of the chemokine eotaxin-1 (CCL11). Herein, we demonstrate an inflammatory driven expression of CCL11 in bone tissue and a novel role of CCL11 in osteoclast migration and resorption. Using an inflammatory bone lesion model and primary cell cultures, we discovered that osteoblasts express CCL11 in vivo and in vitro and that expression increased during inflammatory conditions...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28680628/corrigendum
#17
(no author information available yet)
[This corrects the article DOI: 10.1002/ccr3.755.].
July 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/28655090/-molecular-mechanisms-and-relationship-of-m2-polarized-macrophages-with-early-response-in-multiple-myeloma
#18
X Y Chen, R X Sun, W Y Zhang, T Liu, Y H Zheng, Y Wu
Objective: To investigate the relationship between M2-polarized macrophages and early response in multiple myeloma and its molecular mechanism. Methods: Two hundred and forty bone marrow biopsy tissue were collected and M2-polarized macrophages were stained by anti-CD163 monoclonal antibody. In vitro M2-polarized macrophages were derived from human peripheral blood mononuclear cell or THP-1 cells and identified by flow cytometry. Two myeloma cell lines RPMI 8226 and U266 were co-cultured with M2 macrophages using a transwell system...
June 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28619539/systemic-immunological-changes-in-patients-with-distinct-clinical-outcomes-during-mycobacterium-tuberculosis-infection
#19
Tatiane Figueiredo Morais-Papini, Jordana Grazziela Alves Coelho-Dos-Reis, Ana Paula Barbosa Wendling, Lis Ribeiro do Vale Antonelli, Pryscilla Fanini Wowk, Vânia Luiza Deperon Bonato, Valéria Maria Augusto, Silvana Elói-Santos, Olindo Assis Martins-Filho, Cláudia Martins Carneiro, Andréa Teixeira-Carvalho
BACKGROUND: The lung lesions in an individual infected with tuberculosis (TB) are surprisingly variable and independent of each other. However, there is no circulating biomarker yet able to segregate patients according to the extent of lung lesions. MATERIALS AND METHODS: In this study, the phenotypic and functional profile of leukocytes of patients with active pulmonary tuberculosis (TB) and controls (CO) were fully scrutinized by immunophenotyping assays and in vitro short-term whole blood culture...
May 26, 2017: Immunobiology
https://www.readbyqxmd.com/read/28618453/data-driven-programmatic-approach-to-analysis-of-basophil-activation-tests
#20
Sarita U Patil, Agustin Calatroni, Michael Schneider, Johanna Steinbrecher, Neal Smith, Cecilia Washburn, Alex Ma, Wayne G Shreffler
BACKGROUND: Conventional data analysis of flow cytometry-based basophil activation testing requires repetitive, labor-intensive analysis that hampers efforts to standardize testing for clinical applications. Using an open-source platform, we developed and implemented a programmatic approach to the analysis of the basophil activation test (BAT) by flow cytometry. METHODS: Using the BÜHLMANN FlowCAST® assay, peripheral blood from peanut allergic patients undergoing oral immunotherapy was incubated with peanut allergens (Arah1, Arah2, Arah6, whole peanut extract) and stained with fluorescent antibodies to CCR3 and CD63 for the development of a data-driven programmatic analysis using Bioconductor and R...
June 15, 2017: Cytometry. Part B, Clinical Cytometry
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