keyword
https://read.qxmd.com/read/38534770/the-combination-of-oolonghomobisflavan-b-and-diallyl-disulfide-induces-apoptotic-cell-death-via-67-kda-laminin-receptor-cyclic-guanosine-monophosphate-in-acute-myeloid-leukemia-cells
#1
JOURNAL ARTICLE
Jaehoon Bae, Su-Jin Park
Diallyl disulfide (DADS) is a well-known principal functional component derived from garlic ( Allium sativum ) that has various health benefits. Previously, we identified a 67-kDa laminin receptor, a receptor for oolong tea polyphenol oolonghomobisflavan B (OHBFB). However, its molecular mechanisms still remain to be elucidated. Here, we show that DADS synergistically enhanced the effect of the oolong tea polyphenol oolonghomobisflavan B (OHBFB), which induces apoptosis in acute myeloid leukemia (AML) cancer cells without affecting normal human peripheral blood mononuclear cells (PBMCs)...
March 14, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38533975/cgmp-dependent-kinase-2-na-h-exchanger-nhe3-and-pdz-adaptor-nherf2-co-assemble-in-apical-membrane-microdomains
#2
JOURNAL ARTICLE
Min Luo, Yongjian Liu, Katerina Nikolovska, Brigitte Riederer, Enrico Patrucco, Franz Hofmann, Ursula Seidler
AIM: Trafficking, membrane retention, and signal-specific regulation of the Na+ /H+ exchanger 3 (NHE3) are modulated by the Na+ /H+ Exchanger Regulatory Factor (NHERF) family of PDZ-adapter proteins. This study explored the assembly of NHE3 and NHERF2 with the cGMP-dependent kinase II (cGKII) within detergent-resistant membrane microdomains (DRMs, "lipid rafts") during in vivo guanylate cycle C receptor (Gucy2c) activation in murine small intestine. METHODS: Small intestinal brush border membranes (siBBMs) were isolated from wild type, NHE3-deficient, cGMP-kinase II-deficient, and NHERF2-deficient mice, after oral application of the heat-stable Escherichia coli toxin (STa) analog linaclotide...
March 27, 2024: Acta Physiologica
https://read.qxmd.com/read/38533961/correlates-of-plasma-nt-probnp-cyclic-gmp-ratio-in-heart-failure-with-preserved-ejection-fraction-an-analysis-of-the-relax-trial
#3
JOURNAL ARTICLE
Leonard Chiu, Vineet Agrawal, David Armstrong, Evan Brittain, Sheila Collins, Anna R Hemnes, Joseph A Hill, JoAnn Lindenfeld, Sanjiv J Shah, Lynne W Stevenson, Thomas J Wang, Deepak K Gupta
BACKGROUND: Phosphodiesterases degrade cyclic GMP (cGMP), the second messenger that mediates the cardioprotective effects of natriuretic peptides. High natriuretic peptide/cGMP ratio may reflect, in part, phosphodiesterase activity. Correlates of natriuretic peptide/cGMP in patients with heart failure with preserved ejection fraction are not well understood. Among patients with heart failure with preserved ejection fraction in the RELAX (Phosphodiesterase-5 Inhibition to Improve Clinical Status and Exercise Capacity in Heart Failure With Preserved Ejection Fraction) trial, we examined (1) cross-sectional correlates of circulating NT-proBNP (N-terminal pro-B-type natriuretic peptide)/cGMP ratio, (2) whether selective phosphodiesterase-5 inhibition by sildenafil changed the ratio, and (3) whether the effect of sildenafil on 24-week outcomes varied by baseline ratio...
March 27, 2024: Journal of the American Heart Association
https://read.qxmd.com/read/38533431/role-and-targeting-of-the-agc-kinase-family-in-pulmonary-fibrosis-review
#4
REVIEW
Chao Mei, Tao Chen, Xiangfei Huang, Chenlu Xiong, Shibiao Chen, Yong Li
Pulmonary fibrosis (PF) is a progressive and irreversible pulmonary disease with a high mortality rate and limited treatment options. The cAMP-dependent protein kinase A, cGMP-dependent protein kinase G and phospholipid-dependent protein kinase C, collectively known as AGC kinases, are evolutionarily conserved protein kinases that are widely distributed among eukaryotes. AGC kinases serve a crucial role in a variety of cellular functions and pathological processes, including cancer, diabetes, inflammation and viral infections, where they have been implicated the pathogenesis of PF...
May 2024: Experimental and Therapeutic Medicine
https://read.qxmd.com/read/38530893/feedforward-and-feedback-mechanisms-cooperatively-regulate-rapid-experience-dependent-response-adaptation-in-a-single-thermosensory-neuron-type
#5
JOURNAL ARTICLE
Tyler J Hill, Piali Sengupta
Sensory adaptation allows neurons to adjust their sensitivity and responses based on recent experience. The mechanisms that mediate continuous adaptation to stimulus history over seconds- to hours-long timescales, and whether these mechanisms can operate within a single sensory neuron type, are unclear. The single pair of AFD thermosensory neurons in Caenorhabditis elegans exhibits experience-dependent plasticity in their temperature response thresholds on both minutes- and hours-long timescales upon a temperature upshift...
April 2, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38525817/exploring-the-therapeutic-potential-of-pomegranate-juice-for-uterine-relaxation
#6
JOURNAL ARTICLE
Omar A M Al-Habib, Lina N Adam
BACKGROUND: The effects of pomegranate juice (PJ) and its components on uterine smooth muscle are unknown. Hence, this study unequivocally demonstrates that pomegranate juice (PJ) significantly impacts myometrial function, providing crucial insights into its relaxant properties and their potential therapeutic applications for uterine-related disorders. RESEARCH DESIGN AND METHODS: Rat uterine smooth muscle horn strips were suspended in Krebs solution organ baths...
March 25, 2024: Expert Review of Endocrinology & Metabolism
https://read.qxmd.com/read/38524120/a-mini-review-phosphodiesterases-in-charge-to-balance-intracellular-camp-during-t-cell-activation
#7
REVIEW
Marie Bielenberg, Roberta Kurelic, Stefan Frantz, Viacheslav O Nikolaev
T-cell activation is a pivotal process of the adaptive immune response with 3',5'-cyclic adenosine monophosphate (cAMP) as a key regulator of T-cell activation and function. It governs crucial control over T-cell differentiation and production of pro-inflammatory cytokines, such as IFN-γ. Intriguingly, levels of intracellular cAMP differ between regulatory (Treg) and conventional T-cells (Tcon). During cell-cell contact, cAMP is transferred via gap junctions between these T-cell subsets to mediate the immunosuppressive function of Treg...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38521574/analysis-of-the-response-to-cigarette-smoke-exposure-in-cell-coculture-and-monoculture-based-on-bionic-lung-microfluidic-chips
#8
JOURNAL ARTICLE
Zezhi Li, Boyang Feng, Xiang Li, Junwei Zhao, Kejian Liu, Fuwei Xie, Jianping Xie
BACKGROUND: In vitro toxicity assessment studies with various experimental models and exposure modalities frequently generate diverse outcomes. In the prevalent experimental, aerosol pollutants are dissolved in culture medium through capture for exposure to two-dimensional planar cellular models in multiwell plates via immersion. However, this approach can generate restricted and inconclusive experimental data, significantly constraining the applicability of risk assessment outcomes. Herein, the in vitro cocultivation of lung epithelial and/or vascular endothelial cells was performed using self-designed bionic-lung microfluidic chip housing a gas-concentration gradient generator (GCGG) unit...
April 29, 2024: Analytica Chimica Acta
https://read.qxmd.com/read/38517938/purine-nucleosides-replace-camp-in-allosteric-regulation-of-pka-in-trypanosomatid-pathogens
#9
JOURNAL ARTICLE
Veronica Teresa Ober, George Boniface Githure, Yuri Volpato Santos, Sidney Becker, Gabriel Moya Munoz, Jérôme Basquin, Frank Schwede, Esben Lorentzen, Michael Boshart
Cyclic nucleotide binding domains (CNB) confer allosteric regulation by cAMP or cGMP to many signaling proteins, including PKA and PKG. PKA of phylogenetically distant Trypanosoma is the first exception as it is cyclic nucleotide-independent and responsive to nucleoside analogues (Bachmaier et al., 2019). Here, we show that natural nucleosides inosine, guanosine and adenosine are nanomolar affinity CNB ligands and activators of PKA orthologs of the important tropical pathogens Trypanosoma brucei , Trypanosoma cruzi, and Leishmania ...
March 22, 2024: ELife
https://read.qxmd.com/read/38512139/regional-variations-in-allergen-induced-airway-inflammation-correspond-to-changes-in-soluble-guanylyl-cyclase-heme-and-expression-of-heme-oxygenase-1
#10
JOURNAL ARTICLE
Mamta P Sumi, Rosemary Westcott, Eric Stuehr, Chaitali Ghosh, Dennis J Stuehr, Arnab Ghosh
Asthma is characterized by airway remodeling and hyperreactivity. Our earlier studies determined that the nitric oxide (NO)-soluble guanylyl cyclase (sGC)-cGMP pathway plays a significant role in human lung bronchodilation. However, this bronchodilation is dysfunctional in asthma due to high NO levels, which cause sGC to become heme-free and desensitized to its natural activator, NO. In order to determine how asthma impacts the various lung segments/lobes, we mapped the inflammatory regions of lungs to determine whether such regions coincided with molecular signatures of sGC dysfunction...
March 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38511325/novel-angiogenesis-role-of-glp-1-32-36-to-rescue-diabetic-ischemic-lower-limbs-via-glp-1r-dependent-glycolysis-in-mice
#11
JOURNAL ARTICLE
Yikai Zhang, Shengyao Wang, Qiao Zhou, Yi Xie, Yepeng Hu, Weihuan Fang, Changxin Yang, Zhe Wang, Shu Ye, Xinyi Wang, Chao Zheng
BACKGROUND: Restoring the capacity of endothelial progenitor cells (EPCs) to promote angiogenesis is the major therapeutic strategy of diabetic peripheral artery disease. The aim of this study was to investigate the effects of GLP-1 (glucagon-like peptide 1; 32-36)-an end product of GLP-1-on angiogenesis of EPCs and T1DM (type 1 diabetes) mice, as well as its interaction with the classical GLP-1R (GLP-1 receptor) pathway and its effect on mitochondrial metabolism. METHODS: In in vivo experiments, we conducted streptozocin-induced type 1 diabetic mice as a murine model of unilateral hind limb ischemia to examine the therapeutic potential of GLP-1(32-36) on angiogenesis...
March 21, 2024: Arteriosclerosis, Thrombosis, and Vascular Biology
https://read.qxmd.com/read/38508664/the-nitric-oxide-soluble-guanylate-cyclase-cgmp-pathway-in-pulmonary-hypertension-from-pde5-to-soluble-guanylate-cyclase
#12
REVIEW
Raymond L Benza, Ekkehard Grünig, Peter Sandner, Johannes-Peter Stasch, Gérald Simonneau
The nitric oxide (NO)-soluble guanylate cyclase (sGC)-cyclic guanosine monophosphate (cGMP) pathway plays a key role in the pathogenesis of pulmonary hypertension (PH). Targeted treatments include phosphodiesterase type 5 inhibitors (PDE5i) and sGC stimulators. The sGC stimulator riociguat is approved for the treatment of pulmonary arterial hypertension (PAH) and chronic thromboembolic pulmonary hypertension (CTEPH). sGC stimulators have a dual mechanism of action, enhancing the sGC response to endogenous NO and directly stimulating sGC, independent of NO...
January 31, 2024: European Respiratory Review: An Official Journal of the European Respiratory Society
https://read.qxmd.com/read/38500966/disrupted-propionate-metabolism-evokes-transcriptional-changes-in-the-heart-by-increasing-histone-acetylation-and-propionylation
#13
JOURNAL ARTICLE
Kyung Chan Park, Nicholas T Crump, Niamh Louwman, Steve Krywawych, Yuen Jian Cheong, Iolanda Vendrell, Eleanor K Gill, Mala Gunadasa-Rohling, Kerrie L Ford, David Hauton, Marjorie Fournier, Elisabete Pires, Lydia Watson, Gerald Roseman, James Holder, Andreas Koschinski, Ricardo Carnicer, M Kate Curtis, Manuela Zaccolo, Alzbeta Hulikova, Roman Fischer, Holger B Kramer, James S O McCullagh, Sophie Trefely, Thomas A Milne, Pawel Swietach
Propiogenic substrates and gut bacteria produce propionate, a post-translational protein modifier. In this study, we used a mouse model of propionic acidaemia (PA) to study how disturbances to propionate metabolism result in histone modifications and changes to gene expression that affect cardiac function. Plasma propionate surrogates were raised in PA mice, but female hearts manifested more profound changes in acyl-CoAs, histone propionylation and acetylation, and transcription. These resulted in moderate diastolic dysfunction with raised diastolic Ca2+ , expanded end-systolic ventricular volume and reduced stroke volume...
December 2023: Nat Cardiovasc Res
https://read.qxmd.com/read/38494903/a-modular-and-multi-functional-purification-strategy-that-enables-a-common-framework-for-manufacturing-scale-integrated-and-continuous-biomanufacturing
#14
JOURNAL ARTICLE
Leon P Pybus, Charles Heise, Tibor Nagy, Carmen Heeran, Terri Dover, John Raven, Junichi Kori, Graeme Burton, Hiroshi Sakuyama, Benjamin Hastings, Michelle Lyons, Shinichi Nakai, Jonathan Haigh
Biopharmaceutical manufacture is transitioning from batch to integrated and continuous biomanufacturing (ICB). The common framework for most ICB, potentially enables a global biomanufacturing ecosystem utilizing modular and multi-function manufacturing equipment. Integrating unit operation hardware and software from multiple suppliers, complex supply chains enabled by multiple customized single-use flow paths, and large volume buffer production/storage make this ICB vision difficult to achieve with commercially available manufacturing equipment...
March 18, 2024: Biotechnology Progress
https://read.qxmd.com/read/38492879/skeletal-muscle-atrophy-after-sciatic-nerve-damage-mechanistic-insights
#15
REVIEW
Aarti Yadav, Rajesh Dabur
Sciatic nerve injury leads to molecular events that cause muscular dysfunction advancement in atrophic conditions. Nerve damage renders muscles permanently relaxed which elevates intracellular resting Ca2+ levels. Increased Ca2+ levels are associated with several cellular signaling pathways including AMPK, cGMP, PLC-β, CERB, and calcineurin. Also, multiple enzymes involved in the tricarboxylic acid cycle and oxidative phosphorylation are activated by Ca2+ influx into mitochondria during muscle contraction, to meet increased ATP demand...
March 14, 2024: European Journal of Pharmacology
https://read.qxmd.com/read/38492103/dynamics-of-changes-in-the-camp-cgmp-concentration-ratio-in-the-thymus-and-spleen-of-laboratory-mice-during-vaccination-against-plague-and-tularemia-against-the-background-of-immunomodulation
#16
JOURNAL ARTICLE
V I Dubrovina, O V Yur'eva, A B Pyatidesyatnikova, T P Starovoitova, S V Balakhonov
Vaccine strains Yersinia pestis EV NIIEG at a dose of 103 CFU and Francisella tularensis 15 NIIEG at a dose of 102 CFU induced changes in the concentration of cyclic nucleotides in the thymus and spleen of white mice. Antigen-induced changes in the cAMP/cGMP ratio in immunocompetent organs had a phase or oscillatory character, which seems to be related to the regulation of postvaccination immunoreactivity in the body. Synthetic organoselenium compound 974zh stimulated an increase in the amplitude of cAMP/cGMP oscillations, indicating its stimulating effect on the immunogenic properties of vaccine strains at doses an order of magnitude below the standard doses...
March 16, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38488688/cyclic-nucleotide-induced-bidirectional-long-term-synaptic-plasticity-in-drosophila-mushroom-body
#17
JOURNAL ARTICLE
Daichi Yamada, Andrew M Davidson, Toshihide Hige
Activation of the cAMP pathway is one of the common mechanisms underlying long-term potentiation (LTP). In the Drosophila mushroom body, simultaneous activation of odour-coding Kenyon cells (KCs) and reinforcement-coding dopaminergic neurons activates adenylyl cyclase in KC presynaptic terminals, which is believed to trigger synaptic plasticity underlying olfactory associative learning. However, learning induces long-term depression (LTD) at these synapses, contradicting the universal role of cAMP as a facilitator of transmission...
March 15, 2024: Journal of Physiology
https://read.qxmd.com/read/38481533/spontaneous-signal-generation-by-an-excitable-system-for-cell-migration
#18
REVIEW
Satomi Matsuoka, Koji Iwamoto, Da Young Shin, Masahiro Ueda
Eukaryotic cells exhibit random migration in the absence of extracellular directional cues. This random migration acts as basal motility for various migratory responses such as chemotaxis. The self-organization of random motility requires the internal signals that determine the anterior side of the migrating cell be generated spontaneously from the intrinsic activities of intracellular signaling networks. Recent studies have identified an excitable system as the mechanism of the spontaneous signal generation...
2024: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/38480090/cyclic-nucleotides-the-rise-of-a-family
#19
REVIEW
Mateusz Kwiatkowski, Jinwen Zhang, Wei Zhou, Chris Gehring, Aloysius Wong
Cyclic nucleotides 3',5'-cAMP and 3',5'-cGMP are now established signaling components of the plant cell while their 2',3' positional isomers are increasingly recognized as such. 3',5'-cAMP/cGMP is generated by adenylate cyclases (ACs) or guanylate cyclases (GCs) from ATP or GTP, respectively, whereas 2',3'-cAMP/cGMP is produced through the hydrolysis of double-stranded DNA or RNA by synthetases. Recent evidence suggests that the cyclic nucleotide generating and inactivating enzymes moonlight in proteins with diverse domain architecture operating as molecular tuners to enable dynamic and compartmentalized regulation of cellular signals...
March 12, 2024: Trends in Plant Science
https://read.qxmd.com/read/38478232/quality-control-assessment-of-human-adipose-derived-hydrogels
#20
JOURNAL ARTICLE
Haley Lassiter, Jordan Robinson, Katie Hamel, Jeffrey M Gimble, Trivia Frazier, Cecilia Sanchez
Decellularized human-adipose tissue (hDAT) can serve as an alternative to two-dimensional monolayer culture and current ECM hydrogels due to its unlimited availability and cytocompatibility. A major hurdle in the clinical translation and integration of hDAT and other hydrogels into current in vitro culture processes is adherence to current good manufacturing practices (cGMP). Transferring of innovative technologies, including hydrogels, requires the establishing standardized protocols for quality assurance and quality control (QA/QC) of the material...
2024: Methods in Molecular Biology
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