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https://www.readbyqxmd.com/read/29775207/structural-and-technological-characterisation-of-pectin-extracted-with-sodium-citrate-and-nitric-acid-from-sunflower-heads
#1
Nerea Muñoz-Almagro, Fabián Rico-Rodriguez, Peter J Wilde, Antonia Montilla, Mar Villamiel
An optimisation of temperature, time and extracting agent concentration of pectin extraction from sunflower heads using sodium citrate and nitric acid (SP-SC and SP-NA) was carried out. At optimal conditions, the yield of extraction with nitric acid (SPO-NA) was 2-fold greater than the corresponding with sodium citrate (SPO-SC) (14.3 vs 7.7%, respectively). Regarding pectin structure, the galacturonic acid (GalA) content in both, SPO-SC and SPO-NA, was similar (∼85%). However, SPO-NA showed lower molecular weight (Mw) (88...
May 18, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29759232/synthesis-identification-integration-one-pot-hydrothermal-preparation-of-fluorescent-nitrogen-doped-carbon-nanodots-for-differentiating-nucleobases-with-the-aid-of-multivariate-chemometrics-analysis
#2
Qianfen Zhuang, Wei Cao, Yongnian Ni, Yong Wang
Most of the conventional multidimensional differential sensors currently need at least two-step fabrication, namely synthesis of probe(s) and identification of multiple analytes by mixing of analytes with probe(s), and were conducted using multiple sensing elements or several devices. In the study, we chose five different nucleobases (adenine, cytosine, guanine, thymine, and uracil) as model analytes, and found that under hydrothermal conditions, sodium citrate could react directly with various nucleobases to yield different nitrogen-doped carbon nanodots (CDs)...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29758010/combined-transcriptomics-proteomics-and-bioinformatics-identify-drug-targets-in-spinal-cord-injury
#3
Jure Tica, Elizabeth J Bradbury, Athanasios Didangelos
Spinal cord injury (SCI) causes irreversible tissue damage and severe loss of neurological function. Currently, there are no approved treatments and very few therapeutic targets are under investigation. Here, we combined 4 high-throughput transcriptomics and proteomics datasets, 7 days and 8 weeks following clinically-relevant rat SCI to identify proteins with persistent differential expression post-injury. Out of thousands of differentially regulated entities our combined analysis identified 40 significantly upregulated versus 48 significantly downregulated molecules, which were persistently altered at the mRNA and protein level, 7 days and 8 weeks post-SCI...
May 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29753693/oral-administration-of-5-aminolevulinic-acid-induces-heme-oxygenase-1-expression-in-peripheral-blood-mononuclear-cells-of-healthy-human-subjects-in-combination-with-ferrous-iron
#4
Hidenori Ito, Yoshiaki Nishio, Takeshi Hara, Hidemitsu Sugihara, Tohru Tanaka, Xiao-Kang Li
Heme oxygenase-1 (HO-1) is a major anti-inflammatory enzyme and a key regulator that induces immune tolerance through affecting the differentiation of dendritic cells. The aim of this study is to determine whether the combination of 5-aminolevulinic acid (ALA) and iron induces HO-1 expression in healthy human peripheral blood mononuclear cells (PBMC). The study was an open labeled, non-randomized, non-placebo-controlled trial using healthy male adults and consisted of three parts. Study A aimed to find the peak HO-1 expression at 0, 1, 2, 3, 4, 6, 8, 12, 16, and 24h after administration...
May 10, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29740015/effects-of-5-aminolevulinic-acid-supplementation-on-home-based-walking-training-achievement-in-middle-aged-depressive-women-randomized-double-blind-crossover-pilot-study
#5
Hiroshi Suzuki, Shizue Masuki, Akiyo Morikawa, Yu Ogawa, Yoshi-Ichiro Kamijo, Kiwamu Takahashi, Motowo Nakajima, Hiroshi Nose
Depressive patients often experience difficulty in performing exercise due to physical and psychological barriers. We examined the effects of 5-aminolevulinic acid (ALA) with sodium ferrous citrate (SFC) supplementation during home-based walking training in middle-aged depressive women. Nine outpatients [53 ± 8 (SD) yr] with major depressive disorder participated in the pilot study with randomized, placebo-controlled, double-blind crossover design. They underwent two trials for 7 days, each performing interval walking training (IWT) with ALA + SFC (ALA + SFC) or placebo supplement intake (PLC) intermittently with >a 10-day washout period...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29737516/tga-ftir-study-of-the-pyrolysis-of-sodium-citrate-and-its-effect-on-the-pyrolysis-of-tobacco-and-tobacco-sba-15-mixtures-under-n-2-and-air-atmospheres
#6
A Marcilla, A Gómez-Siurana, M Beltrán, I Martínez-Castellanos, I Blasco, D Berenguer
In this work, the effect of sodium citrate mixed with tobacco, in presence and absence of SBA-15 material, was studied by TGA/FTIR under N2 and air atmospheres. Depending on the atmosphere used, the decomposition of sodium citrate changes considerably at high temperatures, mainly due to the oxidation of the residue. The analysis of the experimental and calculated DTG data of the sodium citrate/SBA-15 mixtures allows the observation of marked widening of the peaks appearing at lower temperatures, due to the presence of SBA-15, while the expected peak at high temperatures in air completely disappears...
May 8, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29727402/targeted-imaging-of-renal-fibrosis-using-antibody-conjugated-gold-nanoparticles-in-renal-artery-stenosis
#7
Xiang-Yang Zhu, Xiangyu Zou, Rahul Mukherjee, Zhicong Yu, Christopher M Ferguson, Wei Zhou, Cynthia H McCollough, Lilach O Lerman
OBJECTIVES: The ability to determine the severity of renal fibrosis, which is involved in most chronic kidney diseases, may be beneficial for monitoring disease progression and management. The aim of this study was to assess a new method involving gold nanoparticles conjugated to an anti-collagen-I antibody (Co-I-AuNPs) as a computed tomography (CT) imaging contrast for the evaluation of renal fibrosis in situ. MATERIALS AND METHODS: Gold nanoparticles conjugated to an anti-collagen-I antibody were prepared using gold chloride reduction with sodium citrate and coated with polyethylene glycol (PEG), and their size was determined by electron microscopy and nanoparticle tracking analysis...
April 28, 2018: Investigative Radiology
https://www.readbyqxmd.com/read/29714000/design-and-evaluation-of-hydrophilic-matrix-system-for-ph-independent-sustained-release-of-weakly-acidic-poorly-soluble-drug
#8
Jinheng Huang, Huaqing Lin, Bingxin Peng, Qianfeng Huang, Fangzhou Shuai, Yanxian Xie
The aim of this research was to design and evaluate a hydrophilic matrix system for sustained release of glipizide, a weakly acidic poor soluble drug. A combination of inclusion complexation and microenvironmental pH modification techniques was utilized to improve the dissolution and pH-independent release of glipizide. Hydroxypropyl-β-cyclodextrin (HP-β-CD) was used as the complexation agent while sodium citrate and magnesium oxide (MgO) were used as model pH modifiers. The hydrophilic matrix tablets were prepared by powder direct compression and evaluated by in vitro dissolution study respectively in pH 6...
April 30, 2018: AAPS PharmSciTech
https://www.readbyqxmd.com/read/29709761/degradation-of-bisphenol-a-through-transition-metals-activating-persulfate-process
#9
Feng Gao, Yijiu Li, Bo Xiang
In this study, the process of transition metals (Fe2+ , Fe0 , Ni2 O3 ) activating persulfate was attempted to degrade aqueous bisphonel A (BPA). Compared with thermal activation mode, significant degradation can be achieved at normal atmospheric temperature in transition metal activation mode. BPA removal in the transition metal-PS system can be divided into rapid phase (0-5 min) and slow phase (5-60 min). In rapid phase, 87.71% and 90.60% removal efficiencies were obtained in the Fe2+ -PS and Ni2 O3 -PS systems, and the contaminant was almost completely oxidized after 60 min...
April 27, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29708764/human-milk-biomarkers-of-secretory-activation-in-breast-pump-dependent-mothers-of-premature-infants
#10
Rebecca Hoban, Aloka L Patel, Clarisa Medina Poeliniz, Ching Tat Lai, Judy Janes, Donna Geddes, Paula P Meier
OBJECTIVE: Mothers of premature infants confront barriers to coming to volume (CTV; ≥500 mL/day mother's own milk [MOM] by postpartum day 14), a strong predictor of continued MOM provision at neonatal intensive care unit (NICU) discharge. We sought to determine concentrations of secretory activation biomarkers (MOM sodium, total protein, lactose, and citrate) during the first 14 postpartum days and to describe relationships among these biomarkers, pumped MOM volume, CTV, and pumping frequency...
April 30, 2018: Breastfeeding Medicine: the Official Journal of the Academy of Breastfeeding Medicine
https://www.readbyqxmd.com/read/29703633/oral-versus-intravenous-iron-supplementation-for-the-treatment-of-iron-deficiency-anemia-in-patients-on-maintenance-hemodialysis-effect-on-fibroblast-growth-factor-23-metabolism
#11
Wataru Fukao, Yukiko Hasuike, Tomo Yamakawa, Kazuhiro Toyoda, Makoto Aichi, Satoko Masachika, Mari Kantou, Shoko Ikeda Takahishi, Takahide Iwasaki, Mana Yahiro, Masatoshi Nanami, Yasuyuki Nagasawa, Takahiro Kuragano, Takeshi Nakanishi
OBJECTIVE: Iron administration affects serum levels of intact (I-) fibroblast growth factor-23 (FGF23) and its cleavage product C-terminal (C-) FGF23 in iron-deficient patients on maintenance hemodialysis (MHD). The objective of this study was to compare the effect of oral or intravenous iron administration on serum levels of I-FGF23 and C-FGF23 in iron-deficient patients on MHD. DESIGN AND METHODS: A prospective randomized study. SUBJECTS: Participants on MHD with severe iron deficiency (n = 61)...
April 24, 2018: Journal of Renal Nutrition
https://www.readbyqxmd.com/read/29679708/the-impact-of-polymers-on-3d-microstructure-and-controlled-release-of-sildenafil-citrate-from-hydrophilic-matrices
#12
Anna Krupa, Zbisław Tabor, Jacek Tarasiuk, Beata Strach, Krzysztof Pociecha, Elżbieta Wyska, Sebastian Wroński, Ewelina Łyszczarz, Renata Jachowicz
Sildenafil citrate has short biological half-life in humans. Thus, matrix tablets of controlled release were designed and prepared by compaction on the basis of hydrophilic polymers, i.e. HPMC, sodium alginate, carbomer, poloxamer and their mixtures. The impact of these polymers on sildenafil release in vitro and its pharmacokinetics in vivo was evaluated. Since drug release rate from hydrophilic matrices can be govern by the porosity of the matrix, the microstructure of tablets was studied using X-ray microcomputed tomography...
April 18, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29674149/sorption-desorption-of-sb-iii-in-different-soils-kinetics-and-effects-of-the-selective-removal-of-hydroxides-organic-matter-and-humic-substances
#13
Jining Li, Hong Hou, Masaaki Hosomi
To examine the Sb(III) retention by three soils with different properties (Ferrosol, Primosol and Isohumosol), kinetic batch experiments were carried out for Sb(III) adsorption-desorption, followed by Sb release using a sequential extraction procedure. In addition, hydroxides, organic matter, and humic substances were selectively removed by washing the soil with oxalate, sodium dithionate-citrate-bicarbonate, H2 O2 , and NaOH. The effects of removing these substances on Sb(III) retention were investigated by comparing the Sb distribution coefficients and desorption rates...
April 13, 2018: Chemosphere
https://www.readbyqxmd.com/read/29670350/colloidal-plasmonic-gold-nanoparticles-and-gold-nanorings-shape-dependent-generation-of-singlet-oxygen-and-their-performance-in-enhanced-photodynamic-cancer-therapy
#14
Yamin Yang, Yue Hu, Henry Du, Lei Ren, Hongjun Wang
Introduction: In recognition of the potentials of gold nanoparticles (Au NPs) in enhanced photodynamic therapy (PDT) for cancer, it is desirable to further understand the shape-dependent surface plasmonic resonance (SPR) properties of various gold nanostructures and evaluate their performances in PDT. Materials and methods: Monodispersed colloidal spherical solid Au NPs were synthesized by UV-assisted reduction using chloroauric acid and sodium citrate, and hollow gold nanorings (Au NRs) with similar outer diameter were synthesized based on sacrificial galvanic replacement method...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29666516/a-streamlined-isolation-method-and-the-autoxidation-profiles-of-tuna-myoglobin
#15
Mala Nurilmala, Hideki Ushio, Shugo Watabe, Yoshihiro Ochiai
Determination of the redox state of myoglobin (Mb) gives useful information for evaluating the quality of tuna meat. To attain this purpose, a fast streamlined method has been established basically based on preparative native gel electrophoresis to isolate Mb from the dark muscle of Pacific bluefin tuna. Crude Mb fraction was prepared from dark muscle by ammonium sulfate saturation fractionation and subsequently Mb was purified by preparative native gel electrophoresis under the isoelectric pH of the Mb, resulting in absorption (or trapping) of all the contaminating proteins in the gel...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29659531/gold-nanoparticles-grafted-with-pll-b-pnipam-interplay-on-thermal-ph-dual-response-and-optical-properties
#16
Hui-Juan Li, Peng-Yun Li, Li-Ying Li, Abdul Haleem, Wei-Dong He
Narrowly distributed poly(l-lysine- b - N -isopropylacrylamide) (PLL- b -PNIPAM) was prepared through ring-opening polymerization of ε-benzyloxycarbonyl-l-lysine N -carboxy-α-amino anhydride and atom transfer radical polymerization of NIPAM, followed with the removal of ε-benzyloxycarbonyl group. Then gold nanoparticles (AuNPs) grafted with PLL- b -PNIPAM (PNIPAM-PLL-AuNPs) were obtained by the reduction of chloroauric acid with sodium citrate in the presence of PLL- b -PNIPAM...
April 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29624078/the-effect-of-3-2-and-3-8-sodium-citrate-on-specialized-coagulation-tests
#17
Franz Ratzinger, Mona Lang, Sabine Belik, Klaus G Schmetterer, Helmuth Haslacher, Thomas Perkmann, Peter Quehenberger
CONTEXT: - Coagulation testing is challenging and depends on preanalytic factors, including the citrate buffer concentration used. OBJECTIVE: - To better estimate preanalytic effects of the citrate buffer concentration in use, the difference between results obtained by samples with 3.2% and 3.8% citrate was evaluated. DESIGN: - In a prospective observational study with 76 volunteers, differences related to the citrate concentration were evaluated...
April 6, 2018: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29619876/inhibitory-effects-of-taraxasterol-and-aqueous-extract-of-taraxacum-officinale-on-calcium-oxalate-crystallization-in-vitro-study
#18
Mahboubeh Yousefi Ghale-Salimi, Maryam Eidi, Nasser Ghaemi, Ramezan Ali Khavari-Nejad
OBJECTIVE: We investigated and compared the effects of taraxasterol, aqueous extract of T. officinale (AET) aerial part, and potassium citrate (PC) on calcium oxalate (CaOx) crystallization in vitro. MATERIALS AND METHODS: CaOx crystallization was induced by adding sodium oxalate to synthetic urine. Taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL), extract (1, 2, 4 and 8 mg/mL), and PC (100, 150, 200 and 350 mg/mL) were subjected to anti-crystallization activities...
November 2018: Renal Failure
https://www.readbyqxmd.com/read/29616124/sodium-citrate-inhibits-the-proliferation-of-human-gastric-adenocarcinoma-epithelia-cells
#19
Yuan Xia, Xulong Zhang, Agula Bo, Juan Sun, Minhui Li
The objective of the present study was to investigate the cytotoxic effects of sodium citrate on human gastric adenocarcinoma epithelia AGS cells. Numerous cytotoxicity-associated sodium citrate-induced effects were assessed, including cell viability and proliferation, cytokine expression and caspase activity. In vitro studies demonstrated that incubation with sodium citrate (>3.125 mM) inhibited AGS cell viability and proliferation in a dose-dependent manner. Incubation with sodium citrate for 24 h revealed that the levels of interleukin-1β (IL-1β), IL-8 and tumor necrosis factor increased with an increasing of dose of sodium citrate, whereas the IL-6 levels exhibited only a slight alteration...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29615020/purification-and-mic-analysis-of-antimicrobial-proteins-from-cucumis-sativus-l-seeds
#20
Raid Al Akeel, Ayesha Mateen, Khalid K Alharbi, Abdullah A Alyousef, Hazem M Al-Mandeel, Rabbani Syed
BACKGROUND: Cucumis sativus L. (cucumber), from the family Cucurbitaceae, is a therapeutic plant with various pharmacological benefits, broadly utilized as a part of complementary medicine (e.g., Unani, Ayurveda, Siddha, and Traditional Chinese). In light of past research discoveries, this plant had been chosen to consider its potential antibacterial action. METHODS: Extracts were purified by dialysis and ion exchange chromatography strategy and then assayed for antibacterial activity against four standard pathogenic bacterial strains known to cause foodborne infections and spoilage of food and herbal drugs...
April 3, 2018: BMC Complementary and Alternative Medicine
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