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https://www.readbyqxmd.com/read/28330216/potential-application-of-waste-from-castor-bean-ricinus-communis-l-for-production-for-xylanase-of-interest-in-the-industry
#1
Polyanna Nunes Herculano, Keila Aparecida Moreira, Raquel Pedrosa Bezerra, Tatiana Souza Porto, Cristina Maria de Souza-Motta, Ana Lúcia Figueiredo Porto
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds(-1) using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28324689/calcium-phosphate-quercetin-nanocomposite-cpqn-a-multi-functional-nanoparticle-having-ph-indicating-highly-fluorescent-and-anti-oxidant-properties
#2
Mousumi Patra, Riya Mukherjee, Milon Banik, Debanjan Dutta, Naznin Ara Begum, Tarakdas Basu
Calcium phosphate quercetin nanocomposite (CPQN) i.e., quercetin entrapped in calcium phosphate nanoparticle was synthesized by a precipitation method at 80°C, taking ammonium hydrogen phosphate, calcium nitrate and quercetin as precursors and sodium citrate as stabilizer. The nanocomposite suspension had different color at different pH values, a property that could render the nanoparticle a pH indicator. Besides color, the particles also had different size, shape, stability and quercetin content with change of pH...
March 14, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28322056/preparation-of-ultrathin-robust-nanohybrid-capsules-through-a-beyond-biomineralization-method
#3
Shaohua Zhang, Zhongyi Jiang, Weilun Qian, Jiafu Shi, Xiaoli Wang, Lei Tang, Hongjian Zou, Hua Liu
Herein, a facile and generic method is developed to prepare ultrathin, robust nanohybrid capsules by manipulating the dynamic structure of supramolecular nanocoatings on CaCO3 sacrificial templates through incorporating multivalent-anion substitution process into biomineralization. Above biomineralization level, multivalent anions, e.g., phosphate, sulfate or citrate, are employed to initiate the assembly of polyamine into continuous (non-segregated) polyamine-anion supramolecular nanocoatings on CaCO3 sacrificial templates...
March 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28300544/pre-analytical-and-analytical-aspects-affecting-clinical-reliability-of-plasma-glucose-results
#4
REVIEW
Sara Pasqualetti, Federica Braga, Mauro Panteghini
The measurement of plasma glucose (PG) plays a central role in recognizing disturbances in carbohydrate metabolism, with established decision limits that are globally accepted. This requires that PG results are reliable and unequivocally valid no matter where they are obtained. To control the pre-analytical variability of PG and prevent in vitro glycolysis, the use of citrate as rapidly effective glycolysis inhibitor has been proposed. However, the commercial availability of several tubes with studies showing different performance has created confusion among users...
March 11, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28296756/transient-appearance-of-edta-dependent-pseudothrombocytopenia-in-a-postoperative-patient-with-sepsis-a-case-report
#5
Xiang Shi, Zhongyuan Lin, Liyan He, Wenchao Li, Lijun Mo, Yinyin Li, Zheng Yang, Wu-Ning Mo
RATIONALE: Ethylenediaminetetraacetic acid-dependent pseudothrombocytopenia (EDTA-PTCP) is a rare phenomenon characterized by spuriously low platelet counts when EDTA reacts with harvested blood. However, to the best of our knowledge, only two cases involving EDTA-PTCP in postoperative patients with sepsis have been reported. Here, we describe a case of EDTA-PTCP that appeared transiently in a postoperative patient with sepsis. PATIENT CONCERNS: A 68-year-old female patient underwent laparoscopic tension-free hernioplasty for incisional hernia...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28280325/novel-route-synthesis-of-porous-and-solid-gold-nanoparticles-for-investigating-their-comparative-performance-as-contrast-agent-in-computed-tomography-scan-and-effect-on-liver-and-kidney-function
#6
Farooq Aziz, Ayesha Ihsan, Aalia Nazir, Ishaq Ahmad, Sadia Zafar Bajwa, Asma Rehman, Abdoulaye Diallo, Waheed S Khan
Gold nanoparticles (GNPs) with dimension in the range of 1-100 nm have a prominent role in a number of biomedical applications like imaging, drug delivery, and cancer therapy owing to their unique optical features and biocompatibility. In this work, we report a novel technique for the synthesis of two types of GNPs namely porous gold nanoparticles (PGNPs) and solid gold nanoparticles (SGNPs). PGNPs of size 35 nm were fabricated by reduction of gold (III) solution with lecithin followed by addition of L-ascorbic acid and tri-sodium citrate, whereas SGNPs with a dimension of 28 nm were prepared by reflux method using lecithin as a single reducing agent...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28273537/an-extracellular-polymeric-substance-quickly-chelates-mercury-ii-with-n-heterocyclic-groups
#7
Franco Baldi, Michele Gallo, Salvatore Daniele, Dario Battistel, Claudia Faleri, Alojz Kodre, Iztok Arčon
A strain of Klebsiella oxytoca DSM 29614 is grown on sodium citrate in the presence of 50 mg l(-1) of Hg as Hg(NO3)2. During growth, the strain produces an extracellular polymeric substance (EPS), constituted by a mixture of proteins and a specific exopolysaccharide. The protein components, derived from the outer membrane of cells, are co-extracted with the extracellular exopolysaccharide using ethanol. The extracted EPS contains 7.5% of Hg (total amount). This indicates that EPS is an excellent material for the biosorption of Hg(2+), through chemical complexation with the EPS components...
February 20, 2017: Chemosphere
https://www.readbyqxmd.com/read/28265356/removal-of-cadmium-from-contaminated-lentinula-edodes-by-optimized-complexation-and-coagulation
#8
Yi Wang, Chen Wang, Wei Cheng, Yinbing Bian, Peng Guo
Heavy metal pollution is a serious problem for Lentinula edodes; however, the treatment of contaminated L. edodes has seldom been studied. This study investigated the removal of cadmium (Cd) from contaminated L. edodes and its lentinan by complexation and coagulation. Some influencing factors, such as pH, medical dosage, and preoxidation were examined. Cd complexation from contaminated L. edodes was shown to be more efficient under acidic conditions (pH 5.0), with a clearance rate of 80.47% in 25 mmol/L EDTA and 78...
March 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28263023/gold-nanoparticles-based-solid-phase-microextraction-coatings-for-determining-organochlorine-pesticides-in-aqueous-environmental-samples
#9
Adrián Gutiérrez-Serpa, Priscilla Rocío-Bautista, Verónica Pino, Francisco Jiménez-Moreno, Ana I Jiménez-Abizanda
The use of solid-phase microextraction coatings based on gold nanoparticles was investigated, focusing the attention on the preparation of nanoparticles with non-classical reduction agents of HAuCl4 such as gallic acid and H2 O2 , rather than the conventional sodium citrate. All nanoparticles were characterized by diode array spectroscopy, whereas novel nanoparticles prepared with gallic acid and H2 O2 were also characterized by microscopic techniques. Solid-phase microextraction coatings were prepared with a layer-by-layer approach...
March 6, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28258359/concentration-of-zinc-copper-iron-calcium-and-magnesium-in-the-serum-tissues-and-urine-of-streptozotocin-induced-mild-diabetic-rat-model
#10
Tahiry Gómez, Leticia Bequer, Angel Mollineda, José L Molina, Alain Álvarez, Mayrelis Lavastida, Sonia Clapés
The present study aimed to investigate, in the streptozotocin-induced mild diabetic rat model, the zinc (Zn), copper (Cu), iron (Fe), calcium (Ca), and magnesium (Mg) concentration in serum, liver, and kidney tissues, and urine samples from adult Wistar rats treated neonatally with streptozotocin (STZ). Diabetes was induced by subcutaneous administration of streptozotocin (100 mg/Kg) in female Wistar rats of 2 days old (STZ, n = 10). Control group (CG, n = 10) received only sodium-citrate buffer. The mineral concentrations were measured by atomic absorption spectrophotometry...
March 3, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28243562/microfluidic-setup-for-on-line-sers-monitoring-using-laser-induced-nanoparticle-spots-as-sers-active-substrate
#11
Oana-M Buja, Ovidiu D Gordan, Nicolae Leopold, Andreas Morschhauser, Jörg Nestler, Dietrich R T Zahn
A microfluidic setup which enables on-line monitoring of residues of malachite green (MG) using surface-enhanced Raman scattering (SERS) is reported. The SERS active substrate was prepared via laser induced synthesis of silver or gold nanoparticles spot on the bottom of a 200 μm inner dimension glass capillary, by focusing the laser beam during a continuous flow of a mixture of silver nitrate or gold chloride and sodium citrate. The described microfluidic setup enables within a few minutes the monitoring of several processes: the synthesis of the SERS active spot, MG adsorption to the metal surface, detection of the analyte when saturation of the SERS signal is reached, and finally, the desorption of MG from the spot...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28242444/surface-plasmon-resonance-based-spectrophotometric-determination-of-medicinally-important-thiol-compounds-using-unmodified-silver-nanoparticles
#12
Sandeep K Vaishnav, Kuleshwar Patel, Kumudini Chandraker, Jyoti Korram, Rekha Nagwanshi, Kallol K Ghosh, Manmohan L Satnami
The determination of thiol based biological molecules and drugs, such as cysteine (Cys) (I), α-lipoic acid (II), and sodium 2-sulfanylethane sulphonate (Mesna (III)) in human plasma are becoming progressively more important due to the growing body of knowledge about their essential role in numerous biological pathways. Herein we demonstrate a sensitive colorimetric sensor for the determination of medicinally important thiol drugs based on aggregation of the citrate capped silver nanoparticles (Ag NPs). This approach exploited the high affinity of thiols towards the Ag NPs surface which could tempt replacement of the citrate shell by the thiolate shell of target molecules, resulting in aggregation of the NPs through intermolecular electrostatic interaction or hydrogen-bonding...
February 21, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28220601/multiple-sclerosis-and-nephrolithiasis-a-matched-case-comparative-study
#13
Vishnu Ganesan, Wen Min Chen, Rajat Jain, Shubha De, Manoj Monga
OBJECTIVE: To compare stone composition and serum/urine biochemistries in stone formers with multiple sclerosis (MS) against stone formers without MS and to examine the association between mobility, methods of bladder emptying, and stone formation. MATERIALS AND METHODS: In this retrospective case-control study, we identified patients diagnosed with multiple sclerosis and kidney stone disease who were seen at our institution between 2001 and 2016. For the first part of the study, up to 2 controls (stone formers without a history of MS) were identified for each case and matched on age, body mass index (BMI), and sex...
February 20, 2017: BJU International
https://www.readbyqxmd.com/read/28219840/effect-of-surface-functionalisation-on-the-interaction-of-iron-oxide-nanoparticles-with-polymerase-chain-reaction
#14
Ayse Beyza Aysan, Zdeněk Knejzlík, Pavel Ulbrich, Marek Šoltys, Aleš Zadražil, František Štěpánek
The combination of nanoparticles with the polymerase chain reaction (PCR) can have benefits such as easier sample handling or higher sensitivity, but also drawbacks such as loss of colloidal stability or inhibition of the PCR. The present work systematically investigates the interaction of magnetic iron oxide nanoparticles (MIONs) with the PCR in terms of colloidal stability and potential PCR inhibition due to interaction between the PCR components and the nanoparticle surface. Several types of MIONs with and without surface functionalisation by sodium citrate, dextran and 3-aminopropyl-triethoxysilane (APTES) were prepared and characterised by Transmission Electron Microscopy (TEM), dynamic light scattering (DLS) and Fourier Transform Infrared (FT-IR) spectroscopy...
February 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28217360/crystal-structure-of-penta-sodium-hydrogen-dicitrate-from-synchrotron-x-ray-powder-diffraction-data-and-dft-comparison
#15
Alagappa Rammohan, James A Kaduk
The crystal structure of penta-sodium hydrogen dicitrate, Na5H(C6H5O7)2, has been solved and refined using synchrotron X-ray powder diffraction data, and optimized using density functional techniques. Each of the two independent citrate anions is joined into a dimer by very strong centrosymmetric O-H⋯O hydrogen bonds, with O⋯O distances of 2.419 and 2.409 Å. Four octa-hedrally coordinated Na(+) ions share edges to form open layers parallel to the ab plane. A fifth Na(+) ion in trigonal-bipyramidal coordination shares faces with NaO6 octahedra on both sides of these layers...
February 1, 2017: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/28212595/preparation-and-characterization-of-gold-nanoparticles-in-the-presence-of-citrate-and-soybean-seed-extract-in-an-acidic-conditions
#16
Ebrahim Izadi, Ali Rasooli, Abolfazl Akbarzadeh, Soodabeh Davaran
Through the present study, an eco-friendly method was used to synthesize the gold nanoparticles (GNPs) by using the sodium citrate and extract of the soybean seed as reducing the agents at PH 3. X-Ray diffraction (XRD) method was used to evaluate the crystal structure of as-synthesized NPs and it's revealed that this method leads to well crystallized GNPs. In order to determine the particle size and their distribution, field emission scanning microscopy (FE-SEM) and dynamic light scattering (DLS) were used...
February 17, 2017: Drug Research
https://www.readbyqxmd.com/read/28212110/the-impact-of-chewing-gum-on-halitosis-parameters-a-systematic-review
#17
Francisco Wilker Mustafa Gomes Muniz, Stephanie Anagnostopoulos Friedrich, Carina Folgearini Silveira, Cassiano Kuchenbecker Rösing
This study aimed to analyze the impact of chewing gum on halitosis parameters. Three databases were searched with the following focused question: 'Can chewing gum additionally reduce halitosis parameters, such as organoleptic scores and volatile sulfur compounds (VSC), when compared to a control treatment'? Controlled clinical trials presenting at least two halitosis measurements (organoleptic scores and/or VSC) were included. Ten studies were included, and different active ingredients were used. One study was performed using a chewing gum without any active ingredient...
February 17, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28205396/can-the-diagnostic-reliability-of-the-thrombin-generation-test-as-a-global-haemostasis-assay-be-improved-the-impact-of-calcium-chloride-concentration
#18
L A Parunov, S S Surov, Y Liang, T K Lee, M V Ovanesov
BACKGROUND: Thrombin generation test (TGT) is a global haemostasis assay with a potential to predict bleeding tendencies and treatment effects in patients with haemophilia. Despite 15 years of clinical research, the diagnostic value of TGT remains controversial, possibly due to suboptimal sensitivity to coagulation deficiencies, robustness and reproducibility. OBJECTIVE: The goal of this study was to explore the effect of calcium chloride (CaCl2 ) concentration on the TGT's response to intrinsic coagulation factors (F) VIII, IX and XIa...
February 15, 2017: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/28181101/preparation-of-hydroxyapatite-collagen-injectable-bone-paste-with-an-anti-washout-property-utilizing-sodium-alginate-part-1-influences-of-excess-supplementation-of-calcium-compounds
#19
Taira Sato, Masanori Kikuchi, Mamoru Aizawa
The anti-washout property, viscosity, and cytocompatibility to an osteoblastic cell line, MG-63, of anti-washout pastes were investigated. Mixing a hydroxyapatite/collagen bone-like nanocomposite (HAp/Col), an aqueous solution of sodium alginate (Na-Alg), which is a paste hardening and lubricant agent, and supplementation of calcium carbonate or calcium citrate (Ca-Cit) as a calcium resource for the hardening reaction realized an injectable bone paste. Adding Ca-Cit at a concentration greater than eight times the Ca(2+) ion concentration to Na-Alg improved the anti-washout property...
March 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28180009/pseudothrombocytopenia-with-multiple-anticoagulant-sample-collection-tubes
#20
REVIEW
Ferenc Kovacs, Marina Varga, Zsolt Pataki, Erzsebet Rigo
The knowledge of pseudothrombocytopenia (PTCP) is important for the accuracy of a clinical assessment and for avoiding unnecessary treatment. An elderly patient was hospitalized with left lung pneumonia. Severe thrombocytopenia [platelet (PLT) number: 18 × 10(9)/L] without any clinical bleeding was found in ethylenediaminetetraacetic acid blood collection tube. PLT measurement was repeated in various anticoagulant [sodium citrate, lithium heparin, disodium oxalate, hirudin, and magnesium sulfate (Mg-sulfate)] sample collection tubes and all of them showed thrombocytopenia except with Mg-sulfate...
December 2016: Interventional Medicine & Applied Science
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