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https://www.readbyqxmd.com/read/27932551/antigen-unmasking-enhances-visualization-efficacy-of-the-oocyte-activation-factor-phospholipase-c-zeta-in-mammalian-sperm
#1
Junaid Kashir, Luke Buntwal, Michail Nomikos, Brian L Calver, Panagiotis Stamatiadis, Peter Ashley, Vyronia Vassilakopoulou, David Sanders, Paul Knaggs, Evangelia Livaniou, Adnan Bunkheila, Karl Swann, F Anthony Lai
STUDY QUESTION: Is it possible to improve clinical visualization of phospholipase C zeta (PLCζ) as a diagnostic marker of sperm oocyte activation capacity and male fertility? SUMMARY ANSWER: Poor PLCζ visualization efficacy using current protocols may be due to steric or conformational occlusion of native PLCζ, hindering antibody access, and is significantly enhanced using antigen unmasking/retrieval (AUM) protocols. WHAT IS KNOWN ALREADY: Mammalian oocyte activation is mediated via a series of intracellular calcium (Ca(2+)) oscillations induced by sperm-specific PLCζ...
December 8, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27931218/scutellaria-barbata-flavonoids-alleviate-memory-deficits-and-neuronal-injuries-induced-by-composited-a%C3%AE-in-rats
#2
Xiao G Wu, Shu S Wang, Hong Miao, Jian J Cheng, Shu F Zhang, Ya Z Shang
BACKGROUND: The aim of the present study was to investigate the effects of Scutellaria barbata flavonoids (SBF) on memory impairment and neuronal injury induced by amyloid beta protein 25-35 in combination with aluminum trichloride (AlCl3) and recombinant human transforming growth factor-β1 (RHTGF-β1) (composited Aβ) in rats. METHODS: The composited Aβ-treated model of Alzheimer's disease (AD)-like memory impairment and neuronal injury was established in male rats by right intracerebroventricular injection of composited Aβ, and the effects of SBF were assessed using this rat model...
December 8, 2016: Behavioral and Brain Functions: BBF
https://www.readbyqxmd.com/read/27921101/the-effect-of-common-anticoagulants-in-detection-and-quantification-of-malaria-parasitemia-in-human-red-blood-cells-by-atr-ftir-spectroscopy
#3
Miguela Martin, David Perez-Guaita, Dean W Andrew, Jack S Richards, Bayden R Wood, Philip Heraud
Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) has the potential to become a new diagnostic tool for malaria and other diseases. For point-of-care testing, the use of ATR-FTIR in malaria diagnosis enables the analysis of blood in the aqueous state, which represents an enormous advantage by minimising the sample preparation by removing the need for cell fixation. Here we report the quantification of malaria parasitemia in human RBCs in their normal physiological aqueous state...
December 6, 2016: Analyst
https://www.readbyqxmd.com/read/27921087/acute-and-chronic-effects-of-gold-nanoparticles-on-sperm-parameters-and-chromatin-structure-in-mice
#4
Mahsa Nazar, Ali Reza Talebi, Mohammad Hosseini Sharifabad, Abolghasem Abbasi, Arezoo Khoradmehr, Amir Hossein Danafar
BACKGROUND: The particles in the range of 1-100 nm are called nanoparticles. Gold nanoparticle is one of the most important metal nanoparticles with wide usage. OBJECTIVE: This study investigated the effects of gold nanoparticles on sperm parameters and chromatin structure in mice. MATERIALS AND METHODS: In this experimental study, 72 male bulb-c mice were divided into 9 groups including: 4 Sham groups (Sc 1-4), 4 experimental groups (Au 1-4), and 1 control group (C)...
October 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27917836/the-crystal-structure-of-dihydrodipicolinate-reductase-from-the-human-pathogenic-bacterium-bartonella-henselae-strain-houston-1-at-2-3%C3%A2-%C3%A3-resolution
#5
Ali R Cala, Maria T Nadeau, Jan Abendroth, Bart L Staker, Alexandra R Reers, Anthony W Weatherhead, Renwick C J Dobson, Peter J Myler, André O Hudson
In bacteria, the second committed step in the diaminopimelate/lysine anabolic pathways is catalyzed by the enzyme dihydrodipicolinate reductase (DapB). DapB catalyzes the reduction of dihydrodipicolinate to yield tetrahydrodipicolinate. Here, the cloning, expression, purification, crystallization and X-ray diffraction analysis of DapB from the human-pathogenic bacterium Bartonella henselae, the causative bacterium of cat-scratch disease, are reported. Protein crystals were grown in conditions consisting of 5%(w/v) PEG 4000, 200 mM sodium acetate, 100 mM sodium citrate tribasic pH 5...
December 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27912968/validation-of-a-thin-layer-chromatography-densitometry-method-for-the-characterization-of-invertase-activity
#6
Justine Ferey, David Da Silva, Sophie Bravo-Veyrat, Pierre Lafite, Richard Daniellou, Benoît Maunit
This paper presents a kinetic study of invertase, a specific fructofuranosidase cloned from the Leishmania major genome. The kinetic parameters of the β-d-fructofuranosidase from Leishmania major (BfrA) were determined using Thin-Layer Chromatography (TLC) and UV-densitometry (TLC@UV) specifically developed for the separation and detection of three carbohydrates namely sucrose, glucose and fructose. Separation was performed on TLC silica gel 60 F254 plates impregnated with sodium bisulphate and citrate and heated prior to development...
November 25, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27905024/potential-anti-genotoxic-effect-of-sodium-butyrate-to-modulate-induction-of-dna-damage-by-tamoxifen-citrate-in-rat-bone-marrow-cells
#7
Haidan M El-Shorbagy
Sodium butyrate (SB) is one of the histone deacetylase inhibitors (HDACi's) that is recently evidenced to have a prooxidant activity and an ability to reduce hydrogen peroxide-induced DNA damage. Since the majority of estrogen receptor positive breast cancer patients are treated with tamoxifen citrate (TC), which exerts well established oxidative and genotoxic effects, thus the basic objective of this study is to determine whether SB could ameliorate or curate tamoxifen citrate-induced oxidative DNA damage and genotoxic effect in vivo through up-regulation of some antioxidant enzymes...
November 30, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27893419/silver-nanoparticles-enhance-the-sensitivity-of-temozolomide-on-human-glioma-cells
#8
Ping Liang, Hongming Shi, Weiguo Zhu, Qunfeng Gui, Ya Xu, Jianfeng Meng, Xiaoyuan Guo, Zhuang Gong, Huaqun Chen
Glioblastoma multiforme (GBM) continues to be associated with a dismal prognosis despite aggressive treatment. Significant efforts are being made to develop new nanotechnology-based therapeutic and diagnostic agents. Nanoparticles can act directly on cancer cells or as drug carriers to enhance the cancer therapeutic effect. In this study, we investigated the effect of silver nanoparticles (AgNPs) on human glioma U251 cells and its role in the combinational use with Temozolomide (TMZ), an imidazotetrazine derivative of the alkylating agent dacarbazine, against glioma cells...
November 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/27860366/the-effect-of-intranasal-sodium-citrate-on-olfaction-in-post-infectious-loss-results-from-a-prospective-placebo-controlled-trial-in-49-patients
#9
K L Whitcroft, M Ezzat, M Cuevas, P Andrews, T Hummel
OBJECTIVES: Free calcium plays an integral role in peripheral olfactory processing, including feedback inhibition. It has therefore been suggested that reduction of intranasal free calcium with buffer solutions such as sodium citrate may improve olfactory function in patients with smell impairment. Several previous studies have supported this hypothesis, particularly in post-infectious olfactory loss. We therefore aimed to determine whether treatment with intranasal sodium citrate improves olfactory function in patients with post-infectious impairment...
November 10, 2016: Clinical Otolaryngology
https://www.readbyqxmd.com/read/27845736/response-surface-methodology-modelling-of-an-aqueous-two-phase-system-for-purification-of-protease-from-penicillium-candidum-pca-1-tt031-under-solid-state-fermentation-and-its-biochemical-characterization
#10
Amaal M Alhelli, Mohd Yazid Abdul Manap, Abdulkarim Sabo Mohammed, Hamed Mirhosseini, Eilaf Suliman, Zahra Shad, Nameer Khairulla Mohammed, Anis Shobirin Meor Hussin
Penicillium candidum (PCA 1/TT031) synthesizes different types of extracellular proteases. The objective of this study is to optimize polyethylene glycol (PEG)/citrate based on an aqueous two-phase system (ATPS) and Response Surface Methodology (RSM) to purify protease from Penicillium candidum (PCA 1/TT031). The effects of different PEG molecular weights (1500-10,000 g/mol), PEG concentration (9%-20%), concentrations of NaCl (0%-10%) and the citrate buffer (8%-16%) on protease were also studied. The best protease purification could be achieved under the conditions of 9...
November 11, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27836383/sonochemical-green-synthesis-of-ag-graphene-nanocomposite
#11
Pınar Acar Bozkurt
Recently, the popularity for green chemistry and chemical process have increased. The approach must comprehensively be considered for these principles in the design of a synthesis method, chemical analysis, or chemical process. Utilization of nontoxic chemicals, environment friendly solvents, and renewable materials are some of the important issues in green synthesis methods. The importance of green synthesis arises in the production of Ag/graphene nanocomposites, due to their future potential applications in nanomedicine and materials engineering...
March 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/27829194/visualisation-of-collagen-in-fixed-skeletal-muscle-tissue-using-fluorescently-tagged-collagen-binding-protein-cna35
#12
Melika Mohammadkhah, Ciaran K Simms, Paula Murphy
Detection and visualisation of Collagen structure are important to understand the relationship between mechanical behaviour and microstructure in skeletal muscle since Collagen is the main structural protein in animal connective tissues, and is primarily responsible for their passive load-bearing properties. In the current study, the direct detection and visualization of Collagen using fluorescently tagged CNA35 binding protein (fused to EGFP or tdTomato) is reported for the first time on fixed skeletal muscle tissue...
November 1, 2016: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/27821372/ninhydrin-sodium-molybdate-chromogenic-analytical-probe-for-the-assay-of-amino-acids-and-proteins
#13
Shivakumar Anantharaman, Nagaraja Padmarajaiah, Naef Ghllab Saeed Al-Tayar, Ashwinee Kumar Shrestha
A sensitive method has been proposed for the quantification of amino acids and proteins using ninhydrin and sodium molybdate as chromogenic substrates in citrate buffer of pH5.6. A weak molybdate-hydrindantin complex plays the role in the formation of Ruhemann's purple. The linear response for the amino acid, amino acid mixture and Bovine serum albumin is between 0.999 and 66.80μM, 1.52 and 38μM and 5 and 100μg/L, respectively. The molar absorptivity of the individual amino acid by the proposed reaction extends from 0...
October 21, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27818933/inhibition-of-citrate-cotransporter-slc13a5-mindy-by-rnai-improves-hepatic-insulin-sensitivity-and-prevents-diet-induced-non-alcoholic-fatty-liver-disease-in-mice
#14
Sebastian Brachs, Angelika F Winkel, Hui Tang, Andreas L Birkenfeld, Bodo Brunner, Kerstin Jahn-Hofmann, Daniel Margerie, Hartmut Ruetten, Dieter Schmoll, Joachim Spranger
OBJECTIVE: Non-alcoholic fatty liver disease is a world-wide health concern and risk factor for cardio-metabolic diseases. Citrate uptake modifies intracellular hepatic energy metabolism and is controlled by the conserved sodium-dicarboxylate cotransporter solute carrier family 13 member 5 (SLC13A5, mammalian homolog of INDY: mINDY). In Drosophila melanogaster and Caenorhabditis elegans INDY reduction decreased whole-body lipid accumulation. Genetic deletion of Slc13a5 in mice protected from diet-induced adiposity and insulin resistance...
November 2016: Molecular Metabolism
https://www.readbyqxmd.com/read/27817143/enhanced-degradation-of-bde209-in-spiked-soil-by-ferrous-activated-persulfate-process-with-chelating-agents
#15
Hongjiang Peng, Liya Xu, Wei Zhang, Lin Liu, Fuwen Liu, Kuangfei Lin, Qiang Lu
In order to maintain the quantity of ferrous ions, two eco-friendly chelating agents (CAs), i.e., sodium citrate (Citrate) and sodium gluconate (Glu), have been introduced into a traditional iron activated sodium persulfate (PS) system (Fe(2+)/PS). The results indicated that the PS/CA/Fe(2+) oxidation could be an effective method for BDE209 removal. Effects of the chelating agents, reagents dosage, and pH were evaluated in batch experiments. Glu was observed to be more effective than citrate. In addition, the rate constants (k 1) of BDE209 removal indicated a quadratic curve relationship with initial persulfate concentrations (k 1 = -0...
November 5, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27810996/annals-express-stability-of-ionized-calcium-measurements-at-concentrations-less-than-0-3-mmol-l-by-point-of-care-blood-gas-instruments-application-for-post-filter-calcium-quality-control-in-patients-with-citrate-anticoagulation-during-extracorporeal-circulation
#16
Maria Averina, Raymond Jakobsen
: BackgroundMeasurements of ionized calcium (Ca(2)(+)) at concentrations less than 0.3 mmol/L are required for post-filter control in patients that receive extracorporeal circulation with sodium citrate anticoagulation. This study evaluates the stability of the Ca(2)(+) measurements at such concentrations. MethodsThe stability of the Ca(2)(+) measurements was tested by measuring daily the external standard Qualicheck concentration 3 s7950, Radiometer (0.22-0.25 mmol/L) by blood gas instruments ABL800 and ABL90, Radiometer...
November 3, 2016: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/27797476/a-chemical-inhibition-method-to-synthetize-highly-crystalline-prussian-blue-analogs-for-sodium-ion-battery-cathodes
#17
Renjie Chen, Yongxin Huang, Man Xie, Ziheng Wang, Yusheng Ye, Li Li, Feng Wu
The nucleation rate plays a critical role in the synthesis of Prussian blue analogs. Rapid precipitation may lead to a large number of vacancies and a large amount of interstitial water in the material, resulting in poor electrochemical performance in batteries. Hence, sodium citrate is used to compete with [Fe(CN)6]4- to slow down the coordination rates of Ni2+ and Mn2+ ions with ferrous cyanide ions. The feasibility of the experiment is also confirmed by theoretical analysis. Benefitting from stable crystal structure and the removal of interstitial water, the as-prepared Na2NixMnyFe(CN)6 sample exhibits a high reversible capacity of 150 mA h g-1...
October 31, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27784865/rapid-multi-residue-analysis-of-pesticides-in-pulses-by-lc-ms-ms
#18
Keiko Iwakoshi, Kenji Otsuka, Yasuhiro Tamura, Sanae Tomizawa, Tamako Masubuchi, Yumiko Yamaki, Yukiko Nakagawa, Ryoko Masuda, Shota Suto, Yoshie Kokaji, Tetsuya Shindo, Ichiro Takano
Rapid multi-residue analysis of pesticides in pulses was developed using LC-MS/MS. Pesticide residues in 5 g of homogenized pulses were extracted with 30 mL of acetonitrile and salted out with 4 g of anhydrous magnesium sulfate and 2 g of sodium chloride in the presence of citrate buffer in a disposable tube. The resulting residues were extracted with 30 mL of acetonitrile, and co-extractives were removed on a handmade four-layer column, consisting of a layer of Z-Sep/C18 (20 mg/50 mg) dry particles on top of a three-layer, custom-made (pre-packed) column (lower bed: 60 mg of PSA, middle bed: 30 mg of GC, and top bed: 60 mg of C18) packed in a 10 mm internal diameter polypropylene column (3 mL)...
2016: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/27783532/thermosensitive-bioadhesive-gels-for-the-vaginal-delivery-of-sildenafil-citrate-in-vitro-characterization-and-clinical-evaluation-in-women-using-clomiphene-citrate-for-induction-of-ovulation
#19
Ghareb M Soliman, Gihan Fetih, Ahmed M Abbas
OBJECTIVE: The objective of this study is to develop and characterize in situ thermosensitive gels for the vaginal administration of sildenafil as a potential treatment of endometrial thinning occurring as a result of using clomiphene citrate for ovulation induction in women with type II eugonadotrophic anovulation. While sildenafil has shown promising results in the treatment of infertility in women, the lack of vaginal pharmaceutical preparation and the side effects associated with oral sildenafil limit its clinical effectiveness...
November 14, 2016: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/27782976/the-use-of-mechanical-bowel-preparation-in-pelvic-reconstructive-surgery-a-randomized-controlled-trial
#20
Amos O Adelowo, Michele R Hacker, Anna M Modest, Costa A Apostolis, Anthony J Disciullo, Katherine J Hanaway, Eman E Elkadry, Peter L Rosenblatt, Kathleen J Rogers, Lekha S Hota
OBJECTIVE: To compare mechanical bowel preparation (MBP) using oral magnesium citrate with sodium phosphate enema to sodium phosphate (NaP) enema alone during minimally invasive pelvic reconstructive surgery. METHODS: We conducted a single-blind, randomized controlled trial of MBP versus NaP in women undergoing minimally invasive pelvic reconstructive surgery. The primary outcome was intraoperative quality of the surgical field. Secondary outcomes included surgeon assessment of bowel handling and patient-reported tolerability symptoms...
October 25, 2016: Female Pelvic Medicine & Reconstructive Surgery
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