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https://www.readbyqxmd.com/read/28731330/mapping-functionally-important-residues-in-the-na-dicarboxylate-cotransporter-nadc1
#1
Claire Colas, Avner Schlessinger, Ana M Pajor
Transporters from the SLC13 family couple the transport of 2-4 Na+ ions with a di- or tricarboxylate, such as succinate or citrate. We have previously modeled mammalian SLC13 members including the Na+/dicarboxylate cotransporter NaDC1 (SLC13A2), based on a structure of the bacterial homolog VcINDY in an inward-facing conformation with one sodium ion bound at the Na1 site. In the present study, we modeled the outward-facing conformation of the rabbit and human NaDC1 (rbNaDC1 and hNaDC1) using an outward-facing model of VcINDY as a template, and identified residues in or near the putative Na2 and Na3 cation binding sites...
July 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28728551/reduced-glutathione-and-glutathione-disulfide-in-the-blood-of-glucose-6-phosphate-dehydrogenase-deficient-newborns
#2
Zhen-Hua Gong, Guo-Li Tian, Qi-Wei Huang, Yan-Min Wang, Hong-Ping Xu
BACKGROUND: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is commonly detected during mass screening for neonatal disease. We developed a method to measure reduced glutathione (GSH) and glutathione disulfide (GSSG) using tandem mass spectrometry (MS/MS) for detecting G6PD deficiency. METHODS: The concentration of GSH and the GSH/GSSG ratio in newborn dry-blood-spot (DBS) screening and in blood plus sodium citrate for test confirmation were examined by MS/MS using labeled glycine as an internal standard...
July 20, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28715251/body-heat-responsive-gelation-of-methylcellulose-formulation-containing-betaine
#3
Yoshiaki Shirata, Asami Wakasa, Kiyoshi Miura, Hironori Nakamura, Yoshinobu Matsumoto, Tomihiro Miyada
We examined a methylcellulose (MC) formulation that gels at body temperature for enteral alimentation. Betaine was found to have a lowering effect on the gelation temperature of the MC solution. The thermal gelation temperature of a body heat-responsive (BHR) gelling MC formulation, consisting of 2% MC, 15% glucose, 1.2% sodium citrate, and 3.5% betaine mixture, was approximately 32 °C, indicating that it could gel in response to body heat. Glucose release from the BHR gels was delayed at 37 °C in an in vitro study...
July 17, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28713789/screening-of-oxalate-degrading-lactic-acid-bacteria-of-food-origin
#4
Nicoletta Murru, Giuseppe Blaiotta, Maria Francesca Peruzy, Serena Santonicola, Raffaelina Mercogliano, Maria Aponte
A screening for oxalate degrading abilities was initially carried on within Lactic Acid Bacteria cultures of different food origin. Seventy-nine strains were drop-inoculated onto MRS agar plates containing calcium oxalate. By comparing colonies diameters, 31 strains were used to inoculate, in parallel, MRS and MRS modified by sodium oxalate addition. Differences in the strains' growth were assessed by colony forming unit counts. For two strains, the growth in oxalate enriched medium was significantly higher; while, for eleven strains an opposite behaviour was recorded...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28697933/a-simple-method-for-point-of-need-extraction-concentration-and-rapid-multi-mycotoxin-immunodetection-in-feeds-using-aqueous-two-phase-systems
#5
Ruben R G Soares, Ana M Azevedo, Pedro Fernandes, Virginia Chu, João P Conde, M Raquel Aires-Barros
The rapid detection of mycotoxins in feed samples is becoming an increasingly relevant challenge for the food production sector, in order to effectively enforce current regulations and assure food and feed safety. To achieve rapid mycotoxin detection, several biosensing strategies have been published, many reaching assay times of the order of a few minutes. However, the vast majority of these rely on sample preparation based on volatile organic solvents, often comprising complex multi-step procedures and devoid of clean-up and/or concentration effects...
July 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28697307/prawn-shell-derived-chitin-nanofiber-membranes-as-advanced-sustainable-separators-for-li-na-ion-batteries
#6
Tianwen Zhang, Bao Shen, Hong-Bin Yao, Tao Ma, Lei-Lei Lu, Fei Zhou, Shu-Hong Yu
Separators, necessary components to isolate cathodes and anodes in Li/Na-ion batteries, are consumed in a large amount per year and thus their sustainability should be concerned for renewable energy storage systems. However, eco-efficient and environmentally friendly fabrication of separators with high mechanical strength, excellent thermal stability and good electrolyte wettability is still challenging. Herein, we reported the fabrication of a new type of separators for Li/Na-ion batteries through the self-assembly of eco-friendly chitin nanofibers derived from prawn shells...
July 11, 2017: Nano Letters
https://www.readbyqxmd.com/read/28687385/the-use-of-cross-linked-chitosan-beads-for-nutrients-nitrate-and-orthophosphate-removal-from-a-mixture-of-p-po4-n-no2-and-n-no3
#7
Tomasz Jóźwiak, Urszula Filipkowska, Paula Szymczyk, Małgorzata Kuczajowska-Zadrożna, Artur Mielcarek
A hydrogel chitosan sorbent ionically cross-linked with sodium citrate and covalently cross-linked with epichlorohydrin was used to remove nutrients from an equimolar mixture of P-PO4, N-NO2 and N-NO3. The scope of the study included, among other things, determination of the influence of pH on nutrient sorption effectiveness, nutrient sorption kinetics as well as determination of the maximum sorption capacity of cross-linked chitosan sorbents regarding P-PO4 (H2PO4(-), HPO4(2-)), N-NO2 (HNO2, NO2(-)), and N-NO3 (NO3(-))...
July 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28681271/cell-cycle-analysis-in-the-rat-external-granular-layer-evaluated-by-several-bromodeoxyuridine-immunoperoxidase-staining-protocols
#8
Vanesa Molina, Lucía Rodríguez-Vázquez, David Owen, Oliver Valero, Joaquín Martí
An important step in bromodeoxyuridine (BrdU) immunohistochemistry is the production of single-stranded DNA to make the incorporated BrdU accessible to the antibodies. This paper examines the effect of distinct DNA denaturation pretreatments (DNase I, sodium citrate buffer, endonuclease Eco RI and exonuclease III, and HCl hydrolysis) on detection of BrdU. We found that all the methods used in the partial denaturation of DNA combined good nuclear immunostaining with acceptable tissue integrity. We also observed that these immunohistochemical protocols revealed a spatial pattern in the distribution of DNA-synthesizing cells within the cerebellar external granular layer (EGL) of 10-day-old rats, allowing us to estimate the fraction of S-phase cells...
July 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28663616/effect-of-pregabalin-premedication-on-the-requirement-of-anesthetic-and-analgesic-drugs-in-laparoscopic-cholecystectomy-randomized-comparison-of-two-doses
#9
Prasoon Gupta, Anudeep Saxena, Lalita Chaudhary
BACKGROUND: Preoperative medication has a vital role in anesthesia. Pregabalin (PG) is a newer drug of gabapentinoid class and is six times more potent than gabapentin. Our study was designed to evaluate the effect of PG as premedication on the perioperative anesthetic requirement and analgesia. MATERIALS AND METHODS: The study was conducted on ninety patients of American Society of Anesthesiologists Grade I and II of age group 20-60 years, allocated to one of the three groups of thirty patients each...
April 2017: Anesthesia, Essays and Researches
https://www.readbyqxmd.com/read/28660084/a-case-of-thermal-esophageal-injury-induced-by-sodium-picosulfate-with-magnesium-citrate
#10
Dong-Hyuk Yang, Byoung Wook Bang, Kye Sook Kwon, Hyung Kil Kim, Yong Woon Shin
Although thermal esophageal injuries caused by hot food or tea have been reported, thermal esophageal injury due to sodium picosulfate with magnesium citrate (PSMC) used for bowel preparation is rarely reported. We report the case of a 56-year-old man who presented with esophageal injury after ingestion of PSMC. Instead of dissolving the PSMC in water before ingestion, he drank water immediately after swallowing PSMC powder. As soon as he drank water, he developed severe chest pain and hematemesis. Upper endoscopy revealed severe hemorrhagic, ulcerative mucosal change from upper to mid-esophagus...
2017: Case Reports in Gastrointestinal Medicine
https://www.readbyqxmd.com/read/28658057/plasma-retention-and-systemic-kinetics-of-90sr-intramuscularly-injected-in-female-nonhuman-primates
#11
Deepesh Poudel, John A Klumpp, Luiz Bertelli, Raymond A Guilmette, Richard R Brey
Thirteen female Rhesus macaques were intramuscularly injected with Sr(NO3)2 diluted in sodium citrate solution. The biokinetic data from these animals were compared against the predictions of the NCRP 156 wound models combined with the ICRP systemic models. It was observed that the activities measured in plasma of these nonhuman primates (NHPs) were consistently lower than those predicted by the default human biokinetic models. The urinary excretion from the NHPs at times immediately after injection was much greater than that in humans...
August 2017: Health Physics
https://www.readbyqxmd.com/read/28655760/silencing-of-solute-carrier-family-13-member-5-disrupts-energy-homeostasis-and-inhibits-proliferation-of-human-hepatocarcinoma-cells
#12
Zhihui Li, Daochuan Li, Eun Yong Choi, Rena Lapidus, Lei Zhang, Shiew-Mei Huang, Paul Shapiro, Hongbing Wang
The solute carrier family 13 member 5 (SLC13A5), a sodium-coupled citrate transporter, plays a key role in importing citrate from the circulation into liver cells. Recent evidence has revealed that SLC13A5 deletion protects mice from high-fat diet-induced hepatic steatosis and that mutation of the SLC13A5 orthologues in D. melanogaster and C. elegans promotes longevity. However, despite the emerging importance of SLC13A5 in energy homeostasis, whether perturbation of SLC13A5 affects the metabolism and malignancy of hepatocellular carcinoma is unknown...
June 27, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28643541/effects-of-ph-and-concentration-of-sodium-citrate-anticoagulant-on-platelet-aggregation-measured-by-light-transmission-aggregometry-induced-by-adenosine-diphosphate
#13
Ksenia Germanovich, Eti Alessandra Femia, Chun Yan Cheng, Natalia Dovlatova, Marco Cattaneo
The 2013 ISTH-SSC guidelines for the standardization of light transmission aggregometry (LTA) were largely based on expert consensus, as studies directly comparing LTA methodologies were lacking. We experimentally tested the cogency of ISTH-SSC recommendations pertaining to use of anticoagulant, in particular whether: (1) buffered citrate (BC) is preferable to unbuffered citrate (C); (2) the two recommended concentrations of sodium citrate (109 and 129 mM) are equivalent in terms of platelet aggregation (PA)...
June 23, 2017: Platelets
https://www.readbyqxmd.com/read/28631902/-comparative-estimation-of-antilithogenic-activity-of-porcine-kidney-derived-biomedical-substance-and-sodium-citrate-in-experimental-urolithiasis
#14
G V Pavlyashik, A Yu Zharikov, V I Kiselev
AIM: to compare the anti-lithogenic activity of biomedical substance derived from freeze-dried porcine kidney and sodium citrate. MATERIALS AND METHODS: The experiments were conducted on Wistar rats divided into three groups of 15 animals each: control group (disease control), comparison group (sodium citrate treatment) and experimental group (treatment with biomedical substance from porcine kidneys). Experimental urolithiasis was modeled using the ethylene glycol model...
June 2017: Urologii︠a︡
https://www.readbyqxmd.com/read/28620442/efficacy-of-nanoceria-for-periodontal-tissues-alteration-in-glutamate-induced-obese-rats-multidisciplinary-considerations-for-personalized-dentistry-and-prevention
#15
Tetyana V Beregova, Karine S Neporada, Maksym Skrypnyk, Tetyana M Falalyeyeva, Nadiya M Zholobak, Oleksandr B Shcherbakov, Mykola Ya Spivak, Rostyslav V Bubnov
BACKGROUND: Nowadays, we face the global epidemic of obesity, that is known to contribute to the development of many diseases, such as the oral cavity pathologies. Dental and oral pathologies are frequently caused by and overlapped with systemic multifactorial diseases such as obesity being its early indicators and risk factors. The aim was to study the influence of nanoceria on periodontal tissues alteration in glutamate (MSG)-induced obese rats. METHODS: We included 52 Wistar rats of both genders and divided into four groups: newborn rats in group 1 (control) received subcutaneously 8 μl/g saline...
March 2017: EPMA Journal
https://www.readbyqxmd.com/read/28612075/sers-active-metal-organic-frameworks-with-embedded-gold-nanoparticles
#16
Xiaolin Cao, Sihui Hong, Zejun Jiang, Yongxin She, Shanshan Wang, Chan Zhang, Hui Li, Fen Jin, Maojun Jin, Jing Wang
Surface-enhanced Raman scattering (SERS) has been widely used in the detection of targets and strongly depends on the interaction and the distance between the targets and nanoparticles. Herein, metal-organic frameworks (MOFs) were first easily synthesized on a large scale via a water bath method, especially Uio-66 and Uio-67. MOFs embedded with gold nanoparticles (AuNPs) for SERS enhancement were successfully fabricated via an impregnation strategy. The synthesized AuNPs/MOF-199, AuNPs/Uio-66, and AuNPs/Uio-67 composites, with LSPR properties and high adsorption capability of MOFs to preconcentrate the analytes close to the surface of the AuNPs, exhibited excellent SERS activity...
July 10, 2017: Analyst
https://www.readbyqxmd.com/read/28597700/engineering-of-a-novel-optimized-platform-for-sublingual-delivery-with-novel-characterization-tools-in-vitro-evaluation-and-in-vivo-pharmacokinetics-study-in-human
#17
Nadia M Morsi, Ghada A Abdelbary, Ahmed H Elshafeey, M Abdallah Ahmed
The aim of this work was to develop a novel and more efficient platform for sublingual drug delivery using mosapride citrate (MSP) as a model drug. The engineering of this delivery system had two stages, the first stage was tuning of MSP physicochemical properties by complexation with pure phosphatidylcholine or phosphatidylinositol enriched soybean lecithin to form MSP-phospholipid complex (MSP-PLCP). Changes in physicochemical properties were assessed and the optimum MSP-PLCP formula was then used for formulation into a flushing resistant platform using two mucoadhesive polymers; sodium alginates and sodium carboxymethylcellulose at different concentrations...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28595126/effect-of-chelating-agents-on-reactive-green-19-decolorization-through-fe-0-activated-persulfate-oxidation-process
#18
Feng Ding, Hong Chen, Sida Zhang, Tongjun Zhao, Na Liu
The effect of chelating agents on the decolorization of Reactive Green 19 (RG19) through Fe(0) activated persulfate (PS/Fe(0)) process was investigated. Though other previous studies reported that chelating agent can enhance the degradation of organic contaminant in Fenton-like system, our finding showed that the presence of chelating agents would chelate free Fe(2+) and minimize free Fe(2+) concentration, which resulted in the retardation of RG19 decolorization. RG19 decolorization decreased to 7%, 21%, and 15% in the presence of sodium citrate, sodium EDTA, and sodium oxalate, compared with control test (without chelating agent, 99%) within 10 min...
June 5, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28580363/targeted-metabolomics-in-colorectal-cancer-a-strategic-approach-using-standardized-laboratory-tests-of-the-blood-and-urine
#19
Katarzyna J Jerzak, Marissa Laureano, Radwa Elsharawi, Peter Kavsak, Kelvin Kw Chan, Sukhbinder K Dhesy-Thind, Kevin Zbuk
BACKGROUND: Glycolytic markers have been detected in colorectal cancer (CRC) using advanced analytical methods. METHODS: Using commercially available assays, by-products of anaerobic metabolism were prospectively measured in the blood and urine of 20 patients with metastatic colorectal cancer (mCRC) and 20 patients with local disease. Twenty-four-hour urine citrate, plasma lactate, ketones, venous blood gas, anion gap, and osmolar gap were investigated. Results of patients with metastatic and local CRC were compared using two-sample t-tests or equivalent nonparametric tests...
2017: Hypoxia
https://www.readbyqxmd.com/read/28574225/three-stream-bicarbonate-based-hemodialysis-solution-delivery-system-revisited-with-an-emphasis-on-some-aspects-of-acid-base-principles
#20
REVIEW
Susie Q Lew, Orly F Kohn, Yuk-Lun Cheng, Carl M Kjellstrand, Todd S Ing
Hemodialysis patients can acquire buffer base (i.e., bicarbonate and buffer base equivalents of certain organic anions) from the acid and base concentrates of a three-stream, dual-concentrate, bicarbonate-based, dialysis solution delivery machine. The differences between dialysis fluid concentrate systems containing acetic acid versus sodium diacetate in the amount of potential buffering power were reviewed. Any organic anion such as acetate, citrate, or lactate (unless when combined with hydrogen) delivered to the body has the potential of being converted to bicarbonate...
June 2017: Artificial Organs
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