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https://www.readbyqxmd.com/read/27134080/cd-2-sensitivity-and-permeability-of-a-low-voltage-activated-ca-2-channel-with-catsper-like-selectivity-filter
#1
Edgar Garza-López, Julio César Chávez, Carmen Santana-Calvo, Ignacio López-González, Takuya Nishigaki
CatSper is a sperm-specific Ca(2+) channel that plays an essential role in the male fertility. However, its biophysical properties have been poorly characterized mainly due to its deficient heterologous expression. As other voltage-gated Ca(2+) channels (CaVs), CatSper possesses a conserved Ca(2+)-selective filter motif ([T/S]x[D/E]xW) in the pore region. Interestingly, CatSper conserves four aspartic acids (DDDD) as the negatively charged residues in this motif while high voltage-activated CaVs have four glutamic acids (EEEE) and low voltage-activated CaVs possess two glutamic acids and two aspartic acids (EEDD)...
July 2016: Cell Calcium
https://www.readbyqxmd.com/read/25656605/impact-of-phosphomimetic-and-non-phosphorylatable-mutations-of-phospholemman-on-l-type-calcium-channels-gating-in-hek-293t-cells
#2
Kai Guo, Yue-Peng Wang, Zhi-Wen Zhou, Yi-Bo Jiang, Wei Li, Xiao-Meng Chen, Yi-Gang Li
BACKGROUND: Phospholemman (PLM) is an important phosphorylation substrate for protein kinases A and C in the heart. Until now, the association between PLM phosphorylation status and L-type calcium channels (LTCCs) gating has not been fully understood. We investigated the kinetics of LTCCs in HEK 293T cells expressing phosphomimetic or nonphosphorylatable PLM mutants. METHODS: The LTCCs gating was measured in HEK 293T cells transfected with LTCC and wild-type (WT) PLM, phosphomimetic or nonphosphorylatable PLM mutants: 6263AA, 6869AA, AAAA, 6263DD, 6869DD or DDDD...
March 2015: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/25140443/dddd-is-a-coa-transferase-lyase-producing-dimethyl-sulfide-in-the-marine-environment
#3
Uria Alcolombri, Paola Laurino, Pedro Lara-Astiaso, Assaf Vardi, Dan S Tawfik
Dimethyl sulfide (DMS) is produced in oceans in vast amounts (>10(7) tons/year) and mediates a wide range of processes from regulating marine life forms to cloud formation. Nonetheless, none of the enzymes that produce DMS from dimethylsulfoniopropionate (DMSP) has been adequately characterized. We describe the expression and purification of DddD from the marine bacterium Marinomonas sp. MWYL1 and its biochemical characterization. We identified DMSP and acetyl-coenzyme A to be DddD's native substrates and Asp602 as the active site residue mediating the CoA-transferase prior to lyase activity...
September 2, 2014: Biochemistry
https://www.readbyqxmd.com/read/23763627/aaaa-dddd-quadruple-hydrogen-bond-arrays-featuring-nh%C3%A2-%C3%A2-%C3%A2-n-and-ch%C3%A2-%C3%A2-%C3%A2-n-hydrogen-bonds
#4
David A Leigh, Craig C Robertson, Alexandra M Z Slawin, Patrick I T Thomson
The X-ray crystal structure of a previously reported extremely strong quadruple NH···N AAAA-DDDD hydrogen-bond array [5·4] (K(a) = 1.5 × 10(6) M(-1) in CH3CN; K(a) > 3 × 10(12) M(-1) in CH2Cl2) features four short linear hydrogen bonds. Changing the two benzimidazole groups of the DDDD unit to triazole groups replaces two of the NH···N hydrogen bonds with CH···N interactions (complex [5·6]), but only reduces the association constant in CH3CN by 2 orders of magnitude (K(a) = 2.6 × 10(4) M(-1) in CH3CN; K(a) > 1 × 10(7) M(-1) in CH2Cl2)...
July 3, 2013: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/23558762/trends-in-opioid-analgesics-sales-to-community-pharmacies-and-hospitals-in-italy-2000-2010
#5
A T Caraceni, C Brunelli, P Rocco, P Minghetti
BACKGROUND: Opioid consumption data in Italy have been widely studied. However, only aggregate data can be found in the published literature, and differences are expected by distribution setting (community pharmacies and hospitals). The aim of our paper is to analyse opioids sales trends in Italy in the decade 2000-2010, in an effort to explore such differences. METHODS: Quarterly sales data of opioid medicinal products sold by wholesalers to both community pharmacies (retail) and to hospitals (non-retail) during the time period 2000-2010 were supplied by IMS Italy...
August 2013: Minerva Anestesiologica
https://www.readbyqxmd.com/read/23166664/the-crystal-structure-of-arabidopsis-vsp1-reveals-the-plant-class-c-like-phosphatase-structure-of-the-dddd-superfamily-of-phosphohydrolases
#6
Yuhong Chen, Jia Wei, Mingzhu Wang, Zhubing Shi, Weimin Gong, Min Zhang
Arabidopsis thaliana vegetative storage proteins, VSP1 and VSP2, are acid phosphatases and belong to the haloacid dehalogenase (HAD) superfamily. In addition to their potential nutrient storage function, they were thought to be involved in plant defense and flower development. To gain insights into the architecture of the protein and obtain clues about its function, we have tested their substrate specificity and solved the structure of VSP1. The acid phosphatase activities of these two enzymes require divalent metal such as magnesium ion...
2012: PloS One
https://www.readbyqxmd.com/read/22664859/destructive-discovertebral-degenerative-disease-of-the-lumbar-spine
#7
A K Charran, G Tony, R Lalam, P N M Tyrrell, B Tins, J Singh, S M Eisenstein, B Balain, J M Trivedi, V N Cassar-Pullicino
The uncommon variant of degenerative hip joint disease, termed rapidly progressive osteoarthritis, and highlighted by severe joint space loss and osteochondral disintegration, is well established. We present a similar unusual subset in the lumbar spine termed destructive discovertebral degenerative disease (DDDD) with radiological features of vertebral malalignment, severe disc resorption, and "bone sand" formation secondary to vertebral fragmentation. Co-existing metabolic bone disease is likely to promote the development of DDDD of the lumbar spine, which presents with back pain and sciatica due to nerve root compression by the "bone sand" in the epidural space...
September 2012: Skeletal Radiology
https://www.readbyqxmd.com/read/22457986/genomic-insights-into-bacterial-dmsp-transformations
#8
REVIEW
Mary Ann Moran, Chris R Reisch, Ronald P Kiene, William B Whitman
Genomic and functional genomic methods applied to both model organisms and natural communities have rapidly advanced understanding of bacterial dimethylsulfoniopropionate (DMSP) degradation in the ocean. The genes for the two main pathways in bacterial degradation, routing DMSP to distinctly different biogeochemical fates, have recently been identified. The genes dmdA, -B, -C, and -D mediate the demethylation of DMSP and facilitate retention of carbon and sulfur in the marine microbial food web. The genes dddD, -L, -P, -Q, -W, and -Y mediate the cleavage of DMSP to dimethylsulfide (DMS), with important consequences for ocean-atmosphere sulfur flux...
2012: Annual Review of Marine Science
https://www.readbyqxmd.com/read/21336332/an-aaaa%C3%A2-dddd-quadruple-hydrogen-bond-array
#9
Barry A Blight, Christopher A Hunter, David A Leigh, Hamish McNab, Patrick I T Thomson
Secondary electrostatic interactions between adjacent hydrogen bonds can have a significant effect on the stability of a supramolecular complex. In theory, the binding strength should be maximized if all the hydrogen-bond donors (D) are on one component and all the hydrogen-bond acceptors (A) are on the other. Here, we describe a readily accessible AAAA–DDDD quadruple hydrogen-bonding array that exhibits exceptionally strong binding for a small-molecule hydrogen-bonded complex in a range of different solvents (K(a) > 3 × 10(12) M(-1) in CH2Cl2, 1...
March 2011: Nature Chemistry
https://www.readbyqxmd.com/read/20880330/dddq-a-novel-cupin-containing-dimethylsulfoniopropionate-lyase-in-marine-roseobacters-and-in-uncultured-marine-bacteria
#10
Jonathan D Todd, Andrew R J Curson, Mark Kirkwood, Matthew J Sullivan, Robert T Green, Andrew W B Johnston
Ruegeria (previously Silicibacter) pomeroyi DSS-3, a marine roseobacter, can catabolize dimethylsulfoniopropionate (DMSP), a compatible solute that is made in large amounts by marine plankton and algae. This strain was known to demethylate DMSP via a demethylase, encoded by the dmdA gene, and it can also cleave DMSP, releasing the environmentally important volatile dimethyl sulfide (DMS) in the process. We found that this strain has two different genes, dddP and dddQ, which encode enzymes that cleave DMSP, generating DMS plus acrylate...
February 2011: Environmental Microbiology
https://www.readbyqxmd.com/read/19966446/2-3-dihydro-1-3-benzothiazol-2-iminium-monohydrogen-sulfate-and-2-iminio-2-3-dihydro-1-3-benzothiazole-6-sulfonate-a-combined-structural-and-theoretical-study
#11
Rafal Kruszynski, Agata Trzesowska-Kruszynska
The 2-aminobenzothiazole sulfonation intermediate 2,3-dihydro-1,3-benzothiazol-2-iminium monohydrogen sulfate, C(7)H(7)N(2)S(+).HSO(4)(-), (I), and the final product 2-iminio-2,3-dihydro-1,3-benzothiazole-6-sulfonate, C(7)H(6)N(2)O(3)S(2), (II), both have the endocyclic N atom protonated; compound (I) exists as an ion pair and (II) forms a zwitterion. Intermolecular N-H...O and O-H...O hydrogen bonds are seen in both structures, with bonding energy (calculated on the basis of density functional theory) ranging from 1...
December 2009: Acta Crystallographica. Section C, Crystal Structure Communications
https://www.readbyqxmd.com/read/19807777/molecular-dissection-of-bacterial-acrylate-catabolism-unexpected-links-with-dimethylsulfoniopropionate-catabolism-and-dimethyl-sulfide-production
#12
Jonathan D Todd, Andrew R J Curson, Nefeli Nikolaidou-Katsaraidou, Charles A Brearley, Nicholas J Watmough, Yohan Chan, Philip C B Page, Lei Sun, Andrew W B Johnston
A bacterium in the genus Halomonas that grew on dimethylsulfoniopropionate (DMSP) or acrylate as sole carbon sources and that liberated the climate-changing gas dimethyl sulfide in media containing DMSP was obtained from the phylloplane of the macroalga Ulva. We identified a cluster that contains genes specifically involved in DMSP catabolism (dddD, dddT) or in degrading acrylate (acuN, acuK) or that are required to break down both substrates (dddC, dddA). Using NMR and HPLC analyses to trace 13C- or 14C-labelled acrylate and DMSP in strains of Escherichia coli with various combinations of cloned ddd and/or acu genes, we deduced that DMSP is imported by the BCCT-type transporter DddT, then converted by DddD to 3-OH-propionate (3HP), liberating dimethyl sulfide in the process...
February 2010: Environmental Microbiology
https://www.readbyqxmd.com/read/19760403/sulfonate-group-modified-feptcu-nanoparticles-as-a-selective-probe-for-ldi-ms-analysis-of-oligopeptides-from-a-peptide-mixture-and-human-serum-proteins
#13
Hideya Kawasaki, Tarui Akira, Takehiro Watanabe, Kazuyoshi Nozaki, Tetsu Yonezawa, Ryuichi Arakawa
Bare FePtCu nanoparticles (NPs) are first prepared for laser desorption/ionization mass spectroscopy (LDI-MS) analysis as affinity probes to selectively trap oppositely charged analytes from a sample solution. Our present results demonstrate bare FePtCu NPs to be a potentially useful matrix for surface-assisted laser desorption/ionization mass spectroscopy (SALDI-MS), for the analysis of small proteins and peptides. The upper detectable mass range of peptides was approximately 5 kDa, and the detection limit for peptides approximately 5 fmol...
November 2009: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/19710707/identification-of-genes-for-dimethyl-sulfide-production-in-bacteria-in-the-gut-of-atlantic-herring-clupea-harengus
#14
Andrew R J Curson, Matthew J Sullivan, Jonathan D Todd, Andrew W B Johnston
Phytoplankton are the primary producers of the sulfur-containing compatible solute dimethylsulfoniopropionate (DMSP). These cells are consumed by mesozooplankton, which, in turn, may be eaten by marine vertebrates. From the gut of one such animal, the Atlantic Herring Clupea harengus, we isolated strains of the gamma-proteobacteria Pseudomonas and Psychrobacter that grew on DMSP as sole carbon source, and which produced the environmentally important sulfurous volatile dimethyl sulfide (DMS). In both bacterial genera, this ability was because of the previously identified gene dddD, which specifies an enzyme that liberates DMS from DMSP...
January 2010: ISME Journal
https://www.readbyqxmd.com/read/19588118/the-potato-r-locus-codes-for-dihydroflavonol-4-reductase
#15
Yongfei Zhang, Shuping Cheng, Darlene De Jong, Helen Griffiths, Rayko Halitschke, Walter De Jong
The potato R locus is required for the production of red pelargonidin-based anthocyanin pigments in potato (Solanum tuberosum L.). Red color also requires tissue-specific regulatory genes, such as D (for expression in tuber skin) and F (expression in flowers). A related locus, P, is required for production of blue/purple anthocyanins; P is epistatic to R. We have previously reported that the dihydroflavonol 4-reductase gene (dfr) co-segregates with R. To test directly whether R corresponds to dfr, we placed the allele of dfr associated with red color under the control of the CaMV 35S promoter and introduced it into the potato cultivar Prince Hairy (genotype dddd rrrr P-), which has white tubers and pale blue flowers...
September 2009: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/19407379/crystallization-and-preliminary-x-ray-crystallographic-analysis-of-a-novel-histidinol-phosphate-phosphatase-from-thermococcus-onnurineus-na1
#16
Ha Il Jung, Hyun Sook Lee, Young Jun An, Yona Cho, Jung Hyun Lee, Sung Gyun Kang, Sun Shin Cha
The TON_0887 gene product from Thermococcus onnurineus NA1 is a 240-residue protein that has histidinol-phosphate phosphatase (HolPase) activity. According to analysis of its primary structure, the TON_0887 gene product is a monofunctional HolPase that belongs to the DDDD superfamily. This contrasts with the generally accepted classification that bifunctional HolPases belong to the DDDD superfamily. The TON_0887 gene product was purified and crystallized at 295 K. A 2.2 A resolution data set was collected using synchrotron radiation...
May 1, 2009: Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
https://www.readbyqxmd.com/read/19346350/coral-associated-bacteria-and-their-role-in-the-biogeochemical-cycling-of-sulfur
#17
Jean-Baptiste Raina, Dianne Tapiolas, Bette L Willis, David G Bourne
Marine bacteria play a central role in the degradation of dimethylsulfoniopropionate (DMSP) to dimethyl sulfide (DMS) and acrylic acid, DMS being critical to cloud formation and thereby cooling effects on the climate. High concentrations of DMSP and DMS have been reported in scleractinian coral tissues although, to date, there have been no investigations into the influence of these organic sulfur compounds on coral-associated bacteria. Two coral species, Montipora aequituberculata and Acropora millepora, were sampled and their bacterial communities were characterized by both culture-dependent and molecular techniques...
June 2009: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/19220400/the-dddp-gene-encoding-a-novel-enzyme-that-converts-dimethylsulfoniopropionate-into-dimethyl-sulfide-is-widespread-in-ocean-metagenomes-and-marine-bacteria-and-also-occurs-in-some-ascomycete-fungi
#18
J D Todd, A R J Curson, C L Dupont, P Nicholson, A W B Johnston
The marine alphaproteobacterium Roseovarius nubinhibens ISM can produce the gas dimethyl sulfide (DMS) from dimethylsulfoniopropionate (DMSP), a widespread secondary metabolite that occurs in many phytoplankton. Roseovarius possesses a novel gene, termed dddP, which when cloned, confers on Escherichia coli the ability to produce DMS. The DddP polypeptide is in the large family of M24 metallopeptidases and is wholly different from two other enzymes, DddD and DddL, which were previously shown to generate DMS from dimethylsulfoniopropionate...
June 2009: Environmental Microbiology
https://www.readbyqxmd.com/read/18845157/structural-insights-into-the-catalytic-mechanism-of-the-bacterial-class-b-phosphatase-apha-belonging-to-the-dddd-superfamily-of-phosphohydrolases
#19
Rosalida Leone, Emilia Cappelletti, Manuela Benvenuti, Gianluca Lentini, Maria Cristina Thaller, Stefano Mangani
AphA is a magnesium-dependent, bacterial class B acid phosphatase that catalyzes the hydrolysis of a variety of phosphoester substrates and belongs to the DDDD superfamily of phosphohydrolases. The recently reported crystal structure of AphA from Escherichia coli has revealed the quaternary structure of the enzyme together with hints about its catalytic mechanism. The present work reports the crystal structures of AphA from E. coli in complex with substrate, transition-state, and intermediate analogues. The structures provide new insights into the mechanism of the enzyme and allow a revision of some aspects of the previously proposed mechanism that have broader implications for all the phosphatases of the DDDD superfamily...
December 12, 2008: Journal of Molecular Biology
https://www.readbyqxmd.com/read/18802629/cyclic-tetraureas-with-variable-flexibility-synthesis-crystal-structures-and-properties
#20
Denys Meshcheryakov, Françoise Arnaud-Neu, Volker Böhmer, Michael Bolte, Julien Cavaleri, Véronique Hubscher-Bruder, Iris Thondorf, Sabine Werner
Macrocyclic molecules containing several amide or urea functions may serve as anion receptors. We describe the synthesis of 32-membered macrocycles, in which four rigid xanthene units (X) and/or diphenyl ether units (D) as flexible analogues are linked via urea groups. All six possible combinations of these units (XXXX, XXXD, XXDD, XDXD, XDDD and DDDD) were synthesized and two examples were characterised by single-crystal X-ray analyses (DDDD and two structures for XXXD). Both macrocycles showed distinct differences in their overall conformation and consequently in their hydrogen-bonding pattern...
September 21, 2008: Organic & Biomolecular Chemistry
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