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https://www.readbyqxmd.com/read/27903396/-role-of-orai1-in-the-balloon-injury-induced-rat-carotid-artery-neointimal-formation-and-the-relationship-between-orai1-and-sodium-and-calcium-exchange-1
#1
S L Huang, L X Yang, S J Yang, R W Guo
Objective: To investigate the role of ORAI1 in the balloon injury-induced rat carotid artery neointimal formation and the relationship between ORAI1 and sodium and calcium exchanger(NCX)1. Methods: According to condition of carotid artery balloon injury and observation time, Sprague Dawley rats were divided into 3 groups including sham group, 7 days injury group, and 14 day injury group(n=3 each). According to virus of transfection, Sprague Dawley rats were divided into 2 groups including negative control group (negative lentiviruses particles transfection after establishment of carotid artery balloon injury model, n=3) and siORAI1 group(siORAI1 transfection after operation, n=3)...
November 24, 2016: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/27890711/the-endogenous-lipid-n-arachidonoyl-glycine-is-hypotensive-and-nitric-oxide-cgmp-dependent-vasorelaxant
#2
Yousuf M Al Suleimani, Ahmed S Al Mahruqi
N-arachidonoyl glycine (NAGLY), is the endogenous lipid that activates the G protein-couple receptor 18 (GPR18) with vasodilatory activity in resistance arteries. This study investigates its hemodynamic effects and mechanisms of vasorelaxation. Hemodynamic effects of NAGLY in rats were assessed using a Biopac system and its vascular responses were assessed using a wire myograph. NAGLY (1 mg/kg) decreased blood pressure by 69.4 ± 5.5% and reduced renal blood flow by 88 ± 12% and the effects were not sensitive to inhibition by O-1918 (3 mg/kg)...
November 24, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27879668/structure-functional-basis-of-ion-transport-in-sodium-calcium-exchanger-ncx-proteins
#3
REVIEW
Moshe Giladi, Reut Shor, Michal Lisnyansky, Daniel Khananshvili
The membrane-bound sodium-calcium exchanger (NCX) proteins shape Ca(2+) homeostasis in many cell types, thus participating in a wide range of physiological and pathological processes. Determination of the crystal structure of an archaeal NCX (NCX_Mj) paved the way for a thorough and systematic investigation of ion transport mechanisms in NCX proteins. Here, we review the data gathered from the X-ray crystallography, molecular dynamics simulations, hydrogen-deuterium exchange mass-spectrometry (HDX-MS), and ion-flux analyses of mutants...
November 22, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27861123/tecrl-a-new-life-threatening-inherited-arrhythmia-gene-associated-with-overlapping-clinical-features-of-both-lqts-and-cpvt
#4
Harsha D Devalla, Roselle Gélinas, Elhadi H Aburawi, Abdelaziz Beqqali, Philippe Goyette, Christian Freund, Marie-A Chaix, Rafik Tadros, Hui Jiang, Antony Le Béchec, Jantine J Monshouwer-Kloots, Tom Zwetsloot, Georgios Kosmidis, Frédéric Latour, Azadeh Alikashani, Maaike Hoekstra, Jurg Schlaepfer, Christine L Mummery, Brian Stevenson, Zoltan Kutalik, Antoine Af de Vries, Léna Rivard, Arthur Am Wilde, Mario Talajic, Arie O Verkerk, Lihadh Al-Gazali, John D Rioux, Zahurul A Bhuiyan, Robert Passier
Genetic causes of many familial arrhythmia syndromes remain elusive. In this study, whole-exome sequencing (WES) was carried out on patients from three different families that presented with life-threatening arrhythmias and high risk of sudden cardiac death (SCD). Two French Canadian probands carried identical homozygous rare variant in TECRL gene (p.Arg196Gln), which encodes the trans-2,3-enoyl-CoA reductase-like protein. Both patients had cardiac arrest, stress-induced atrial and ventricular tachycardia, and QT prolongation on adrenergic stimulation...
December 1, 2016: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/27856160/anti-excitotoxic-effects-of-cannabidiol-are-partly-mediated-by-enhancement-of-ncx2-and-ncx3-expression-in-animal-model-of-cerebral-ischemia
#5
Sepideh Khaksar, Mohammad Reza Bigdeli
Excitotoxicity and imbalance of sodium and calcium homeostasis trigger pathophysiologic processes in cerebral ischemia which can accelerate neuronal death. Neuroprotective role of cannabidiol (CBD), one of the main non-psychoactive phytocannabinoids of the cannabis plant, has attracted attention of many researchers in the neurodegenerative diseases studies. The present investigation was designed to determine whether cannabidiol can alleviate the severity of ischemic damages and if it is able to exert its anti-excitotoxic effects through sodium and calcium regulation...
November 14, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27832106/the-effect-of-a-novel-highly-selective-inhibitor-of-the-sodium-calcium-exchanger-ncx-on-cardiac-arrhythmias-in-in-vitro-and-in-vivo-experiments
#6
Zsófia Kohajda, Nikolett Farkas-Morvay, Norbert Jost, Norbert Nagy, Amir Geramipour, András Horváth, Richárd S Varga, Tibor Hornyik, Claudia Corici, Károly Acsai, Balázs Horváth, János Prorok, Balázs Ördög, Szilvia Déri, Dániel Tóth, Jouko Levijoki, Piero Pollesello, Tuula Koskelainen, Leena Otsomaa, András Tóth, István Baczkó, István Leprán, Péter P Nánási, Julius Gy Papp, András Varró, László Virág
BACKGROUND: In this study the effects of a new, highly selective sodium-calcium exchanger (NCX) inhibitor, ORM-10962 were investigated on cardiac NCX current, Ca2+ transients, cell shortening and in experimental arrhythmias. The level of selectivity of the novel inhibitor on several major transmembrane ion currents (L-type Ca2+ current, major repolarizing K+ currents, late Na+ current, Na+/K+ pump current) was also determined. METHODS: Ion currents in single dog ventricular cells (cardiac myocytes; CM), and action potentials in dog cardiac multicellular preparations were recorded utilizing the whole-cell patch clamp and standard microelectrode techniques, respectively...
2016: PloS One
https://www.readbyqxmd.com/read/27816547/overexpression-of-na-ca-2-exchanger-1-display-enhanced-relaxation-in-the-gastric-fundus
#7
Yasuyuki Fujimoto, Satomi Hayashi, Yasu-Taka Azuma, Kazunori Mukai, Kazuhiro Nishiyama, Satomi Kita, Ai Morioka, Hidemitsu Nakajima, Takahiro Iwamoto, Tadayoshi Takeuchi
In gastric smooth muscles, the released Ca(2+) activates the contractile proteins and Ca(2+) taken up from the cytosol cause relaxation. The Na(+)/Ca(2+) exchanger (NCX) is an antiporter membrane protein that controls Ca(2+) influx and efflux across the membrane. However, the possible relation of NCX in gastric fundus motility is largely unknown. Here, we investigated electric field stimulation (EFS)-induced relaxations in the circular muscles of the gastric fundus in smooth muscle-specific NCX1 transgenic mice (Tg)...
November 2016: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/27805378/characterization-of-the-cation-binding-sites-in-the-nckx2-na-ca-2-k-exchanger
#8
Hristina Rumenova Zhekova, Chunfeng Zhao, Paul P M Schnetkamp, Sergei Yu Noskov
NCKX1-5 are proteins involved in the K(+) dependent Na(+)/Ca(2+) exchange in various signal tissues. Here we present a homology model of NCKX2 based on the crystal structure of the NCX_Mj transporter found in Methanoccocus jannaschii. Molecular dynamics simulations were performed on the resultant wild type NCKX2 model and two mutants (D548N and D575N) loaded with either 4Na(+) or 1Ca(2+)-1K(+) ions, in line with the experimentally observed transport stoichiometry. The selectivity of the active site in the wild type NCKX2 for Na(+), K(+), and Li(+) as well as the electrostatic interactions of the positive Na(+) ions in the negatively charged active site of the wild type NCKX2 and the two mutants were evaluated from Free Energy Perturbation calculations...
November 2, 2016: Biochemistry
https://www.readbyqxmd.com/read/27798130/mechanisms-of-co2-h-sensitivity-of-astrocytes
#9
Egor Turovsky, Shefeeq M Theparambil, Vitaliy Kasymov, Joachim W Deitmer, Ana Gutierrez Del Arroyo, Gareth L Ackland, Jason J Corneveaux, April N Allen, Matthew J Huentelman, Sergey Kasparov, Nephtali Marina, Alexander V Gourine
: Ventral regions of the medulla oblongata of the brainstem are populated by astrocytes sensitive to physiological changes in PCO2/[H(+)]. These astrocytes respond to decreases in pH with elevations in intracellular Ca(2+) and facilitated exocytosis of ATP-containing vesicles. Released ATP propagates Ca(2+) excitation among neighboring astrocytes and activates neurons of the brainstem respiratory network triggering adaptive increases in breathing. The mechanisms linking increases in extracellular and/or intracellular PCO2/[H(+)] with Ca(2+) responses in chemosensitive astrocytes remain unknown...
October 19, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27792163/the-control-of-calcium-metabolism-in-zebrafish-danio-rerio
#10
REVIEW
Chia-Hao Lin, Pung-Pung Hwang
Zebrafish is an emerging model for the research of body fluid ionic homeostasis. In this review, we focus on current progress on the regulation of Ca(2+) uptake in the context of Ca(2+) sensing and hormonal regulation in zebrafish. Na⁺-K⁺-ATPase-rich cells (NaRCs), the specialized ionocytes in the embryonic skin and adult gills, play a dominant role in Ca(2+) uptake in zebrafish. Transepithelial Ca(2+) transport in NaRC, through apical epithelial Ca(2+) channels (ECaC), basolateral plasma membrane Ca(2+)-ATPase (PMCA), and Na⁺/Ca(2+) exchanger (NCX), is analogous to mammalian renal and intestinal Ca(2+)-absorption cells...
October 26, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27782859/mesenchymal-stromal-cells-derived-from-cervical-cancer-produce-high-amounts-of-adenosine-to-suppress-cytotoxic-t-lymphocyte-functions
#11
María de Lourdes Mora-García, Rosario García-Rocha, Omar Morales-Ramírez, Juan José Montesinos, Benny Weiss-Steider, Jorge Hernández-Montes, Luis Roberto Ávila-Ibarra, Christian Azucena Don-López, Marco Antonio Velasco-Velázquez, Vianey Gutiérrez-Serrano, Alberto Monroy-García
BACKGROUND: In recent years, immunomodulatory mechanisms of mesenchymal stem/stromal cells (MSCs) from bone marrow and other "classic" sources have been described. However, the phenotypic and functional properties of tumor MSCs are poorly understood. The aim of this study was to analyze the immunosuppressive capacity of cervical cancer-derived MSCs (CeCa-MSCs) on effector T lymphocytes through the purinergic pathway. METHODS: We determined the expression and functional activity of the membrane-associated ectonucleotidases CD39 and CD73 on CeCa-MSCs and normal cervical tissue-derived MSCs (NCx-MSCs)...
October 26, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27754251/os-36-02-astragaloside-iv-ameliorated-mitochondrial-oxidative-stress-in-hypertensive-mice-with-cardiomyopathy-by-regulating-mitochondrial-ca2-flux
#12
Qiongying Wang, An Xie, Han Xu, Jing Yu
OBJECTIVE: Abnormal Ca handling is thought to be related with triggered activity and mitochondria participate in Ca homeostasis. We evaluated the contribution of Astragaloside IV (ASI) for mitochondrial ROS and Ca Flux to cardiac function by using DOCA-salt hypertensive mice. DESIGN AND METHOD: We used the deoxycorticosterone acetate (DOCA)-salt mouse model. The ventricular myocytes isolated from mice was utilized for action potentials (APs) recording, potassium and L-type Ca currents...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27747307/neuronal-na-channels-are-integral-components-of-pro-arrhythmic-na-ca-2-signaling-nanodomain-that-promotes-cardiac-arrhythmias-during-%C3%AE-adrenergic-stimulation
#13
Przemysław B Radwański, Hsiang-Ting Ho, Rengasayee Veeraraghavan, Lucia Brunello, Bin Liu, Andriy E Belevych, Sathya D Unudurthi, Michael A Makara, Silvia G Priori, Pompeo Volpe, Antonis A Armoundas, Wolfgang H Dillmann, Bjorn C Knollmann, Peter J Mohler, Thomas J Hund, Sándor Györke
BACKGROUND: Cardiac arrhythmias are a leading cause of death in the US. Vast majority of these arrhythmias including catecholaminergic polymorphic ventricular tachycardia (CPVT) are associated with increased levels of circulating catecholamines and involve abnormal impulse formation secondary to aberrant Ca(2+) and Na(+) handling. However, the mechanistic link between β-AR stimulation and the subcellular/molecular arrhythmogenic trigger(s) remains elusive. METHODS AND RESULTS: We performed functional and structural studies to assess Ca(2+) and Na(+) signaling in ventricular myocyte as well as surface electrocardiograms in mouse models of cardiac calsequestrin (CASQ2)-associated CPVT...
June 2016: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/27723783/increase-in-cardiac-ischemia-reperfusion-injuries-in-opa1-mouse-model
#14
Sophie Le Page, Marjorie Niro, Jérémy Fauconnier, Laura Cellier, Sophie Tamareille, Abdallah Gharib, Arnaud Chevrollier, Laurent Loufrani, Céline Grenier, Rima Kamel, Emmanuelle Sarzi, Alain Lacampagne, Michel Ovize, Daniel Henrion, Pascal Reynier, Guy Lenaers, Delphine Mirebeau-Prunier, Fabrice Prunier
BACKGROUND: Recent data suggests the involvement of mitochondrial dynamics in cardiac ischemia/reperfusion (I/R) injuries. Whilst excessive mitochondrial fission has been described as detrimental, the role of fusion proteins in this context remains uncertain. OBJECTIVES: To investigate whether Opa1 (protein involved in mitochondrial inner-membrane fusion) deficiency affects I/R injuries. METHODS AND RESULTS: We examined mice exhibiting Opa1delTTAG mutations (Opa1+/-), showing 70% Opa1 protein expression in the myocardium as compared to their wild-type (WT) littermates...
2016: PloS One
https://www.readbyqxmd.com/read/27718571/assessment-of-dft-functionals-for-qtaim-topological-analysis-of-halogen-bonds-with-benzene
#15
Alessandra Forni, Stefano Pieraccini, Davide Franchini, Maurizio Sironi
Halogen bonding, a noncovalent interaction between a halogen atom and a nucleophilic site, is receiving a growing attention in the chemical community stimulating a large number of theoretical investigations. The DFT approach revealed to be one of the most suitable methods owing to its accuracy and low computational cost. We report here a detailed analysis of the performance of an extensive set of DFT functionals in reproducing accurate binding energies and topological properties for the halogen bonding interaction of either NCX or PhX molecules (X = F, Cl, Br, I) with the aromatic system of benzene in the T-shaped configuration...
October 10, 2016: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/27694214/dyssynchronous-calcium-removal-in-heart-failure-induced-atrial-remodeling
#16
F Hohendanner, J DeSantiago, F R Heinzel, L A Blatter
We tested the hypothesis that in atrial myocytes from a rabbit left ventricular heart failure (HF) model, spatial inhomogeneity and temporal dyssynchrony of Ca removal during excitation-contraction coupling together with increased Na/Ca exchange (NCX) activity generate a substrate for proarrhythmic Ca release. Ca removal occurs via Ca reuptake into the sarcoplasmic reticulum and extrusion via NCX exclusively in the cell periphery since rabbit atrial myocytes lack transverse tubules. Ca removal kinetics were assessed by the time constant τ of decay of local peripheral subsarcolemmal (SS) and central (CT) action potential (AP)-induced Ca transients (CaTs) recorded in confocal line scan mode (using Fluo-4)...
December 1, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27679508/hepatectomy-for-colorectal-cancer-liver-metastases-in-the-era-of-modern-preoperative-chemotherapy-evaluation-of-postoperative-complications
#17
Kengo Fukuoka, Satoshi Nara, Yoshitaka Honma, Yoji Kishi, Minoru Esaki, Kazuaki Shimada
BACKGROUND: Recently, an increasing number of patients with liver metastases from colorectal cancer have received chemotherapy before hepatectomy. However, the effect of chemotherapy on postoperative short-term outcome is not well defined. METHODS: We retrospectively investigated the postoperative complications of 439 patients who underwent hepatectomy for colorectal liver metastases in our division from 2005 to 2014. Patients were classified into two groups according to the presence (Cx; 84 patients) or absence (NCx; 355 patients) of preoperative chemotherapy...
September 27, 2016: World Journal of Surgery
https://www.readbyqxmd.com/read/27673688/suppressive-effect-of-carvedilol-on-na-ca2-exchange-current-in-isolated-guinea-pig-cardiac-ventricular-myocytes
#18
Miyuki Tashiro, Yasuhide Watanabe, Tomomi Yamakawa, Kanna Yamashita, Satomi Kita, Takahiro Iwamoto, Junko Kimura
BACKGROUND AND AIMS: Carvedilol ((+/-)-1-(carbazol-4-yloxy)-3-[[2-(o-methoxyphenoxy)ethyl]amino]-2-propanol), a β-adrenoceptor-blocker, has multi-channel blocking and vasodilator properties. This agent dose-dependently improves left ventricular function and reduces mortality in patients with arrhythmia and chronic heart failure. However, the effect of carvedilol on the cardiac Na+/Ca2+ exchanger (NCX1) has not been investigated. METHODS AND RESULTS: We examined the effects of carvedilol and metoprolol, 2 β-blockers, on Na+/Ca2+ exchange current (INCX) in guinea-pig cardiac ventricular cells and fibroblasts expressing dog cardiac NCX1...
September 28, 2016: Pharmacology
https://www.readbyqxmd.com/read/27665854/swimming-exercise-reverses-aging-related-contractile-abnormalities-of-female-heart-by-improving-structural-alterations
#19
Nihal Ozturk, Yusuf Olgar, Hakan Er, Murathan Kucuk, Semir Ozdemir
BACKGROUND: The objective of this study was to examine the effect of swimming exercise on aging-related Ca2+ handling alterations and structural abnormalities of female rat heart. METHODS: For this purpose, 4-month and 24-month old female rats were used and divided into three following groups: sedentary young (SY), sedentary old (SO), and exercised old (Ex-O). Swimming exercise was performed for 8 weeks (60 min/day, 5 days/week). Myocyte shortening, L-type Ca2+ currents and associated Ca2+ transients were measured from ventricular myocytes at 36 ± 1°C...
September 26, 2016: Cardiology Journal
https://www.readbyqxmd.com/read/27643216/os-36-02-astragaloside-iv-ameliorated-mitochondrial-oxidative-stress-in-hypertensive-mice-with-cardiomyopathy-by-regulating-mitochondrial-ca2-flux
#20
Qiongying Wang, An Xie, Han Xu, Jing Yu
OBJECTIVE: Abnormal Ca handling is thought to be related with triggered activity and mitochondria participate in Ca homeostasis. We evaluated the contribution of Astragaloside IV (ASI) for mitochondrial ROS and Ca Flux to cardiac function by using DOCA-salt hypertensive mice. DESIGN AND METHOD: We used the deoxycorticosterone acetate (DOCA)-salt mouse model. The ventricular myocytes isolated from mice was utilized for action potentials (APs) recording, potassium and L-type Ca currents...
September 2016: Journal of Hypertension
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