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https://www.readbyqxmd.com/read/28934732/long-noncoding-rna-cir-promotes-chondrocyte-extracellular-matrix-degradation-in-osteoarthritis-by-acting-as-a-sponge-for-mir-27b
#1
Yu-Fei Li, Shu-Hua Li, Yong Liu, Ya-Tong Luo
BACKGROUND/AIMS: Osteoarthritis (OA) is a degenerative joint disease characterized by articular cartilage degradation. The degradation of the extracellular matrix (ECM) of chondrocyte is closely associated with the destruction of joints in OA patients. lncRNAs are non-coding segments of RNA that possess important regulatory functions at the cellular level and in a variety of pathophysiological processes. The present study was conducted to investigate whether lncRNA-CIR regulated the expression of MMP13 as a sponge of miR-27 in OA...
September 21, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28934730/dihydroartemisinin-and-curcumin-synergistically-induce-apoptosis-in-skov3-cells-via-upregulation-of-mir-124-targeting-midkine
#2
Jiaojiao Zhao, Yuchen Pan, Xiujun Li, Xuefang Zhang, Yaxian Xue, Tingting Wang, Shuli Zhao, Yayi Hou
BACKGROUND/AIM: Women with advanced ovarian carcinoma are less likely to receive platinum-based chemotherapy and surgery due to a greater risk of cytotoxicity and poorer outcomes. We attempted to improve a promising therapy against ovarian cancer by using a combination of dihydroartemisinin (DHA) and curcumin (Cur). METHODS: Human ovarian cancer SKOV3 cells were treated with DHA, Cur alone, or a combination of both. The viability of SKOV3 cells was measured by Cell Counting Kit-8 (CCK-8) and a colony formation assay...
September 21, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28934714/efficacy-of-dehydroepiandrosterone-dhea-to-overcome-the-effect-of-ovarian-ageing-ditto-a-proof-of-principle-double-blinded-randomized-placebo-controlled-trial
#3
Amarin Narkwichean, Walid Maalouf, Miriam Baumgarten, Lukasz Polanski, Nick Raine-Fenning, Bruce Campbell, Kannamannadiar Jayaprakasan
OBJECTIVE: To evaluate the effect of DHEA supplementation on In-Vitro Fertilisation (IVF) outcome as assessed by ovarian response, oocyte developmental competence and live birth rates in women predicted to have poor ovarian reserve (OR). The feasibility of conducting a large trial is also assessed by evaluating the recruitment rates and compliance of the recruited participants with DHEA/placebo intake and follow-up rates. STUDY DESIGN: A single centre, double blinded, placebo controlled, randomized trial was performed over two years with 60 women undergoing in-vitro fertilisation (IVF)...
September 8, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28934643/combined-effects-of-microcystin-and-nitrite-on-the-growth-lipid-peroxidation-and-antioxidant-responses-of-the-freshwater-rotifer-brachionus-calyciflorus
#4
Ye Liang, Xinglan Chen, Xuxin Lu, Sichen Jin, Yuanqi Min, Jiaxin Yang
Toxicants released during the degradation of cyanobacterial blooms, such as microcystin-LR (MC-LR) and nitrite (NO2-N), affect the growth of aquatic organisms. The freshwater rotifer Brachionus calyciflorus was exposed to solutions with different combined concentrations of MC-LR (0, 10, 50, 100, and 200μgL(-1)) and NO2-N (0, 2, 4, 6, and 8mgL(-1)) to assess the combined effects of MC-LR and NO2-N on life cycle parameters and oxidative stress. Single solutions of MC-LR 200μgL(-1) and NO2-N 8mgL(-1) were toxic to rotifers...
September 14, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28934642/physiological-responses-and-gene-expression-changes-in-the-western-mosquitofish-gambusia-affinis-exposed-to-progesterone-at-environmentally-relevant-concentrations
#5
Liping Hou, Hongyan Xu, Guangguo Ying, Yang Yang, Hu Shu, Jianliang Zhao, Xuemei Cheng
Progesterone (P4) is a natural and synthetic steroid, widely distributed in the aquatic environments. It can lead to adverse effects on the endocrine system in aquatic organisms. This study investigated the toxicological effects of exposure to environmentally relevant concentrations (4, 44, and 410ng/L) of progesterone for 42 d on adult female mosquitofish, Gambusia affinis. We performed morphological and histological analyses on gonads, anal fins, liver, and gills after the exposure of mosquito fish to P4...
September 14, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28934520/damage-of-inner-ear-sensory-hair-cells-via-mitochondrial-loss-in-a-murine-model-of-sleep-apnea-with-chronic-intermittent-hypoxia
#6
Young Joon Seo, Hyun Mi Ju, Sun Hee Lee, Sang Hyun Kwak, Min Jung Kang, Joo-Heon Yoon, Chang-Hoon Kim, Hyung-Ju Cho
Study Objectives: Investigating the exact pathophysiology of obstructive sleep apnea syndrome (OSAS)-induced hearing loss is critical. We sought to verify the hypothesis that a correlation exists between mitochondrial dysfunction in inner ear hair cells and the auditory dysfunction induced by chronic intermittent hypoxia (CIH) in a murine model of sleep apnea. Methods: C57BL/6J adult male mice were randomized to 4 weeks of CIH (n = 12) or normoxia (Sham) (n = 12)...
September 1, 2017: Sleep
https://www.readbyqxmd.com/read/28934469/hnrnp-l-controls-hpv16-rna-polyadenylation-and-splicing-in-an-akt-kinase-dependent-manner
#7
Naoko Kajitani, Jacob Glahder, Chengjun Wu, Haoran Yu, Kersti Nilsson, Stefan Schwartz
Inhibition of the Akt kinase activates HPV16 late gene expression by reducing HPV16 early polyadenylation and by activating HPV16 late L1 mRNA splicing. We identified 'hot spots' for RNA binding proteins at the early polyA signal and at splice sites on HPV16 late mRNAs. We observed that hnRNP L was associated with sequences at all HPV16 late splice sites and at the early polyA signal. Akt kinase inhibition resulted in hnRNP L dephosphorylation and reduced association of hnRNP L with HPV16 mRNAs. This was accompanied by an increased binding of U2AF65 and Sam68 to HPV16 mRNAs...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28934383/overexpression-of-micrornas-from-the-gtl2-rian-locus-contributes-to-postnatal-death-in-mice
#8
Soichiro Kumamoto, Nozomi Takahashi, Kayo Nomura, Makoto Fujiwara, Megumi Kijioka, Yoshinobu Uno, Yoichi Matsuda, Yusuke Sotomaru, Tomohiro Kono
The Dlk1-Dio3 imprinted domain functions in embryonic development but the roles of noncoding RNAs expressed from this domain remain unclear. We addressed this question by generating transgenic (TG) mice harbouring a BAC carrying IG-DMR (intergenic-differentially methylated region), Gtl2-DMR, Gtl2, Rtl1/Rtl1as, and part of Rian. High postnatal lethality (>85%) of the BAC-TG pups was observed in the maternally transmitted individuals (MAT-TG), but not following paternal transmission (PAT-TG). The DNA methylation status of IG-DMR and Gtl2-DMR in the BAC-allele was paternally imprinted similar to the genomic allele...
October 1, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28934294/immunopathology-of-childhood-celiac-disease-key-role-of-intestinal-epithelial-cells
#9
Grzegorz Pietz, Rituparna De, Maria Hedberg, Veronika Sjöberg, Olof Sandström, Olle Hernell, Sten Hammarström, Marie-Louise Hammarström
BACKGROUND & AIMS: Celiac disease is a chronic inflammatory disease of the small intestine mucosa due to permanent intolerance to dietary gluten. The aim was to elucidate the role of small intestinal epithelial cells in the immunopathology of celiac disease in particular the influence of celiac disease-associated bacteria. METHODS: Duodenal biopsies were collected from children with active celiac disease, treated celiac disease, and clinical controls. Intestinal epithelial cells were purified and analyzed for gene expression changes at the mRNA and protein levels...
2017: PloS One
https://www.readbyqxmd.com/read/28934292/hawaiian-skirt-controls-size-and-floral-organ-number-by-modulating-cuc1-and-cuc2-expression
#10
Zinnia H González-Carranza, Xuebin Zhang, Janny L Peters, Veronique Boltz, Judit Szecsi, Mohammed Bendahmane, Jeremy A Roberts
The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT (HWS) affects organ growth and the timing of floral organ abscission. The loss-of-function hws-1 mutant exhibits fused sepals and increased organ size. To understand the molecular mechanisms of HWS during plant development, we mutagenized hws-1 seeds with ethylmethylsulphonate (EMS) and screened for mutations suppressing hws-1 associated phenotypes. We isolated the shs1/hws-1 (suppressor of hws-1) mutant in which hws-1 sepal fusion phenotype was suppressed...
2017: PloS One
https://www.readbyqxmd.com/read/28934278/intercalated-disc-in-failing-hearts-from-patients-with-dilated-cardiomyopathy-its-role-in-the-depressed-left-ventricular-function
#11
Ana Ortega, Estefanía Tarazón, Carolina Gil-Cayuela, María García-Manzanares, Luis Martínez-Dolz, Francisca Lago, José Ramón González-Juanatey, Juan Cinca, Esther Jorge, Manuel Portolés, Esther Roselló-Lletí, Miguel Rivera
Alterations in myocardial structure and reduced cardiomyocyte adhesions have been previously described in dilated cardiomyopathy (DCM). We studied the transcriptome of cell adhesion molecules in these patients and their relationships with left ventricular (LV) function decay. We also visualized the intercalated disc (ID) structure and organization. The transcriptomic profile of 23 explanted LV samples was analyzed using RNA-sequencing (13 DCM, 10 control [CNT]), focusing on cell adhesion genes. Electron microscopy analysis to visualize ID structural differences and immunohistochemistry experiments of ID proteins was also performed...
2017: PloS One
https://www.readbyqxmd.com/read/28934261/light-entrainment-of-the-murine-intraocular-pressure-circadian-rhythm-utilizes-non-local-mechanisms
#12
Shunsuke Tsuchiya, Ethan D Buhr, Tomomi Higashide, Kazuhisa Sugiyama, Russell N Van Gelder
PURPOSE: Intraocular pressure (IOP) is known to have a strong circadian rhythm, yet how light/dark cycles entrain this rhythm is unknown. The purpose of this study was to assess whether, like the retina, the mammalian ciliary body and IOP clocks have an intrinsic ability to entrain to light/dark cycles. METHODS: Iris-ciliary body complexes were obtained from period2:luciferase (PER2::LUC) mice and cultured to measure bioluminescence rhythmicity. Pairs of the iris-ciliary body complex were exposed to antiphasic 9:15 h light/dark cycle in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28934230/e-cadherin-a-determinant-molecule-associated-with-ovarian-cancer-progression-dissemination-and-aggressiveness
#13
Marina Rosso, Blanca Majem, Laura Devis, Lara Lapyckyj, María José Besso, Marta Llauradó, María Florencia Abascal, María Laura Matos, Lucia Lanau, Josep Castellví, José Luis Sánchez, Asunción Pérez Benavente, Antonio Gil-Moreno, Jaume Reventós, Anna Santamaria Margalef, Marina Rigau, Mónica Hebe Vazquez-Levin
Ovarian cancer (OC) is the fifth cancer death cause in women worldwide. The malignant nature of this disease stems from its unique dissemination pattern. Epithelial-to-mesenchymal transition (EMT) has been reported in OC and downregulation of Epithelial cadherin (E-cadherin) is a hallmark of this process. However, findings on the relationship between E-cadherin levels and OC progression, dissemination and aggressiveness are controversial. In this study, the evaluation of E-cadherin expression in an OC tissue microarray revealed its prognostic value to discriminate between advanced- and early-stage tumors, as well as serous tumors from other histologies...
2017: PloS One
https://www.readbyqxmd.com/read/28934139/phyllodulcin-a-natural-sweetener-regulates-obesity-related-metabolic-changes-and-fat-browning-related-genes-of-subcutaneous-white-adipose-tissue-in-high-fat-diet-induced-obese-mice
#14
Eunju Kim, Soo-Min Lim, Min-Soo Kim, Sang-Ho Yoo, Yuri Kim
Phyllodulcin is a natural sweetener found in Hydrangea macrophylla var. thunbergii. This study investigated whether phyllodulcin could improve metabolic abnormalities in high-fat diet (HFD)-induced obese mice. Animals were fed a 60% HFD for 6 weeks to induce obesity, followed by 7 weeks of supplementation with phyllodulcin (20 or 40 mg/kg body weight (b.w.)/day). Stevioside (40 mg/kg b.w./day) was used as a positive control. Phyllodulcin supplementation reduced subcutaneous fat mass, levels of plasma lipids, triglycerides, total cholesterol, and low-density lipoprotein cholesterol and improved the levels of leptin, adiponectin, and fasting blood glucose...
September 21, 2017: Nutrients
https://www.readbyqxmd.com/read/28934090/adipogenic-effects-and-gene-expression-profiling-of-firemaster%C3%A2-550-components-in-human-primary-preadipocytes
#15
Emily W Y Tung, Vian Peshdary, Remi Gagné, Andrea Rowan-Carroll, Carole L Yauk, Adéle Boudreau, Ella Atlas
BACKGROUND: Exposure to flame retardants has been associated with negative health outcomes including metabolic effects. As polybrominated diphenyl ether flame retardants were pulled from commerce, human exposure to new flame retardants such as Firemaster® 550 (FM550) has increased. Although previous studies in murine systems have shown that FM550 and its main components increase adipogenesis, the effects of FM550 in human models have not been elucidated. OBJECTIVES: The objectives of this study were to determine if FM550 and its components are active in human preadipocytes, and to further investigate their mode of action...
September 14, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28934014/nucleoplasmic-nup98-controls-gene-expression-by-regulating-a-dexh-d-box-protein
#16
Juliana S Capitanio, Ben Montpetit, Richard W Wozniak
The nucleoporin Nup98 has been linked to the regulation of transcription and RNA metabolism, (1-3) but the mechanisms by which Nup98 contributes to these processes remains largely undefined. Recently, we uncovered interactions between Nup98 and several DExH/D-box proteins (DBPs), a protein family well-known for modulating gene expression and RNA metabolism. (4-6) Analysis of Nup98 and one of these DBPs, DHX9, showed that they directly interact, their association is facilitated by RNA, and Nup98 binding stimulates DHX9 ATPase activity...
September 21, 2017: Nucleus
https://www.readbyqxmd.com/read/28933980/panduratin-a-prevents-tumor-necrosis-factor-alpha-induced-muscle-atrophy-in-l6-rat-skeletal-muscle-cells
#17
Bo-Kyung Sa, Changhee Kim, Mi-Bo Kim, Jae-Kwan Hwang
Panduratin A, a prenylated chalcone compound, has anti-obesity, anti-bacterial, anti-cancer, anti-inflammatory, and anti-oxidative activities. However, its preventive effect on muscle atrophy has not been studied. The purpose of this study was to evaluate the inhibitory effect of panduratin A on muscle atrophy and to investigate its molecular mechanisms in tumor necrosis factor-alpha (TNF-α)-treated L6 rat skeletal muscle cells. Panduratin A restored the myotube diameter reduced by TNF-α. At the molecular level, panduratin A elevated phosphatidylinositol 3 kinase/Akt/mammalian target of rapamycin pathway and stimulated the MyoD and myogenin mRNA expression decreased by TNF-α...
September 21, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28933632/influence-of-light-and-food-on-the-circadian-clock-in-liver-of-rainbow-trout-oncorhynchus-mykiss
#18
Juan Hernández-Pérez, Jesús M Míguez, Fatemeh Naderi, José L Soengas, Marcos A López-Patiño
Several reports support the existence of multiple peripheral oscillators in fish, which may be able to modulate the rhythmic functions developed by those tissues hosting them. Thus, a circadian oscillator has been proposed to be located within fish liver. In this vertebrate group, the role played by the circadian system in regulating metabolic processes in liver is mostly unknown. We, therefore investigated the liver of rainbow trout (Oncorhynchus mykiss) as a potential element participating in the regulation of circadian rhythms in fish by hosting a functional circadian oscillator...
September 21, 2017: Chronobiology International
https://www.readbyqxmd.com/read/28933615/role-of-dicer1-in-thyroid-cell-proliferation-and-differentiation
#19
Ricardo Cortez Cardoso Penha, Romina Sepe, Marco De Martino, Francesco Esposito, Simona Pellecchia, Maddalena Raia, Luigi Del Vecchio, Myriam Decaussin-Petrucci, Gabriella De Vita, Luis Felipe Ribeiro Pinto, Alfredo Fusco
DICER1 plays a central role in the biogenesis of microRNAs and it is important for normal development. Altered microRNA expression and DICER1 dysregulation have been described in several types of tumors, including thyroid carcinomas. Recently, our group identified a new somatic mutation (c.5438A>G; E1813G) within DICER1 gene of an unknown function. Herein, we show that DICER1 is overexpressed, at mRNA level, in a significant-relative number of papillary (70%) and anaplastic (42%) thyroid carcinoma samples, whereas is drastically downregulated in all the analyzed human thyroid carcinoma cell lines (TPC-1, BCPAP, FRO and 8505c) in comparison with normal thyroid tissue samples...
September 21, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28933561/ckap2-promotes-ovarian-cancer-proliferation-and-tumorigenesis-through-the-fak-erk-pathway
#20
Mei Zhang, Longjun Zhao
Upregulation of cytoskeleton-associated protein 2 (CKAP2) has been observed in ovarian cancer. This study aimed to determine the effects of overexpression of CKAP2 on cell proliferation and tumorigenesis and to explore the potential signal pathway in the oncogenic process. Expression of CKAP2 in tissue of ovarian cancer patients was detected. Human cancer cell line SKOV3 was overexpressed with CKAP2 and measured for cell growth and mobility. Moreover, CKAP2 effect was estimated using mouse tumor xenografts...
September 21, 2017: DNA and Cell Biology
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