Read by QxMD icon Read

w hydroxylation

Ameer Khusro, Chirom Aarti, Abdelfattah Z M Salem, German Buendía-Rodríguez, Raymundo Rene Rivas-Cáceres
The desideratum aim of the present context was to isolate a promising antagonist probiotic bacterium from fermented food item as biocontrol agent against uropathogens. Among diversified isolates evaluated for antagonistic trait, Staphylococcus succinus strain AAS2 was found to be an auspicious candidate against urinary tract infection (UTI) causing bacterial pathogens, being the most active against Staphylococcus aureus with substantial activity of 352.5 ± 5.4 AU/mL. Further, the in vitro probiotic attributes of strain AAS2 was assessed using systematic methodology...
March 14, 2018: Microbial Pathogenesis
Michelle N Goettge, Joel P Cioni, Kou-San Ju, Katharina Pallitsch, William W Metcalf
Several oxime-containing small molecules have useful properties, including antimicrobial, insecticidal, anticancer, and immunosuppressive activities. Phosphonocystoximate and its hydroxylated congener, hydroxyphosphonocystoximate, are recently discovered oxime-containing natural products produced by Streptomyces sp. NRRL S-481 and Streptomyces regensis NRRL WC-3744, respectively. The biosynthetic pathways for these two compounds are proposed to diverge at an early step in which 2-aminoethylphosphonate (2AEPn) is converted to (S)-1-hydroxy-2- aminoethylphosphonate ((S)-1H2AEPn) in S...
March 14, 2018: Journal of Biological Chemistry
David Angeles-Albores, Carmie Puckett Robinson, Brian A Williams, Barbara J Wold, Paul W Sternberg
RNA-sequencing (RNA-seq) is commonly used to identify genetic modules that respond to perturbations. In single cells, transcriptomes have been used as phenotypes, but this concept has not been applied to whole-organism RNA-seq. Also, quantifying and interpreting epistatic effects using expression profiles remains a challenge. We developed a single coefficient to quantify transcriptome-wide epistasis that reflects the underlying interactions and which can be interpreted intuitively. To demonstrate our approach, we sequenced four single and two double mutants of Caenorhabditis elegans From these mutants, we reconstructed the known hypoxia pathway...
March 12, 2018: Proceedings of the National Academy of Sciences of the United States of America
Gelavizh Barzegar, Sahand Jorfi, Vahid Zarezade, Masoumeh Khatebasreh, Fayyaz Mehdipour, Farshid Ghanbari
In this study, nanoscale-zero valent iron (nZVI) was synthesized and its function was assessed in ultrasound (US)/peroxymonosulfate (PMS)/nZVI process to degrade 4-chlorphenol (4-CP). The influential operation parameters of US/PMS/nZVI were evaluated on 4-CP degradation. 95% of 4-CP was degraded during 30 min under the conditions of pH = 3.0, nZVI = 0.4 g/L, PMS = 1.25 mM, US power = 200 W. The rate constants of 4-CP degradation for US/PMS/nZVI, PMS/nZVI, US/PMS and US/nZVI were 0.1159, 0...
February 27, 2018: Chemosphere
Martine I Abboud, Tom E McAllister, Ivanhoe K H Leung, Rasheduzzaman Chowdhury, Christian Jorgensen, Carmen Domene, Jasmin Mecinović, Kerstin Lippl, Rebecca L Hancock, Richard J Hopkinson, Akane Kawamura, Timothy D W Claridge, Christopher J Schofield
Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(ii)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ∼50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding.
March 9, 2018: Chemical Communications: Chem Comm
Fabio Tonin, Isabel W C E Arends
Ursodeoxycholic acid (UDCA) is a pharmaceutical ingredient widely used in clinics. As bile acid it solubilizes cholesterol gallstones and improves the liver function in case of cholestatic diseases. UDCA can be obtained from cholic acid (CA), which is the most abundant and least expensive bile acid available. The now available chemical routes for the obtainment of UDCA yield about 30% of final product. For these syntheses several protection and deprotection steps requiring toxic and dangerous reagents have to be performed, leading to the production of a series of waste products...
2018: Beilstein Journal of Organic Chemistry
L Asoka Panamgama, P R U S K Peramune
The structural and thermal features of coconut coir pith lignin, isolated by three different extraction protocols incorporating two different energy supply sources, were characterized by different analytical tools. The three different chemical extraction protocols were alkaline - 7.5% (w/v) NaOH, organosolv - 85% (v/v) formic and acetic acids at 7:3 (v/v) ratio and polyethylene glycol (PEG): water ratio at 80:20 wt%. The two sources of energy were thermal or microwave. Raw lignins were modified by epichlorohydrin to enhance reactivity, and the characteristics of raw and modified lignins were comparatively analysed...
March 5, 2018: International Journal of Biological Macromolecules
Anna Eichinger, Marta K Danecka, Tamara Möglich, Julia Borsch, Mathias Woidy, Lars Büttner, Ania C Muntau, Søren W Gersting
Metabolic control of phenylalanine concentrations in body fluids is essential for cognitive development and executive function. The hepatic phenylalanine hydroxylating system is regulated by the ratio of l-phenylalanine, which is substrate of phenylalanine hydroxylase (PAH), to the PAH cofactor tetrahydrobiopterin (BH4). Physiologically, phenylalanine availability is governed by nutrient intake, whereas liver BH4 is kept at constant level. In phenylketonuria, PAH deficiency leads to elevated blood phenylalanine and is often caused by PAH protein misfolding with loss of function...
March 5, 2018: Human Molecular Genetics
Chunlian Tian, Dexian Zhang, Caixia Yang, Zimo Chen, Mingchun Liu
This paper described the extraction procedure and determination method for the total flavonoids in ethanol extract from the leaves of Abutilon theophrasti Medic., and evaluated antibacterial and antioxidant activity. Maximum extraction yield was achieved using 60% ethanol, 1 : 30 (g/mL) of a ratio of material to solvent, 20 min of extraction time, 40 kHz of ultrasonic frequency, 100 W of ultrasonic power, 600C of extraction temperature and two extraction cycles. Total flavonoids content was 16.79 RE mg/g medicinal materials...
May 2017: Acta Poloniae Pharmaceutica
Yujing Qin, Qingxia Yuan, Yuexing Zhang, Jialu Li, Xinjiao Zhu, Lingling Zhao, Jing Wen, Jikai Liu, Longyan Zhao, Jinhua Zhao
Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369...
March 6, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Yogan Khatri, Ilona K Jóźwik, Michael Ringle, Irina Alexandra Ionescu, Martin Litzenburger, Michael Christopher Hutter, Andy-Mark W H Thunnissen, Rita Bernhardt
The production of regio- and stereoselectively hydroxylated steroids is of high pharmaceutical interest and can be achieved by cytochrome P450-based biocatalysts. CYP260A1 from Sorangium cellulosum strain So ce56 catalyzes hydroxylation of C19 or C21 steroids at the very unique 1-position. However, the conversion of progesterone (PROG) by CYP260A1 is very unselective. In order to improve its selectivity we applied a semi-rational protein engineering approach, resulting in two different, highly regio- and stereoselective mutants by replacing a single serine residue (S276) of the substrate recognition site 5 with an asparagine or isoleucine...
March 6, 2018: ACS Chemical Biology
B L Ehlmann, K S Edgett, B Sutter, C N Achilles, M L Litvak, M G A Lapotre, R Sullivan, A A Fraeman, R E Arvidson, D F Blake, N T Bridges, P G Conrad, A Cousin, R T Downs, T S J Gabriel, R Gellert, V E Hamilton, C Hardgrove, J R Johnson, S Kuhn, P R Mahaffy, S Maurice, M McHenry, P-Y Meslin, D W Ming, M E Minitti, J M Morookian, R V Morris, C D O'Connell-Cooper, P C Pinet, S K Rowland, S Schröder, K L Siebach, N T Stein, L M Thompson, D T Vaniman, A R Vasavada, D F Wellington, R C Wiens, A S Yen
The Mars Science Laboratory Curiosity rover performed coordinated measurements to examine the textures and compositions of aeolian sands in the active Bagnold dune field. The Bagnold sands are rounded to subrounded, very fine to medium sized (~45-500 μm) with ≥6 distinct grain colors. In contrast to sands examined by Curiosity in a dust-covered, inactive bedform called Rocknest and soils at other landing sites, Bagnold sands are darker, less red, better sorted, have fewer silt-sized or smaller grains, and show no evidence for cohesion...
December 2017: Journal of Geophysical Research. Planets
J Zhang, K X Liu, W Gao, X S Xie
Objective: To establish a rat model of paraquat (PQ) -induced pulmonary fibrosis and observe the changes in thrombospondin-1 (TSP-1) and its receptor CD47 in lung tissue, and to investigate their roles in the pathogenesis of PQ-induced pulmonary fibrosis. Methods: Fifty-four clean adult male Sprague-Dawley rats were randomly divided into normal control group ( n =6) and 2 h, 12 h, 1 d, 3 d, 7 d, and 14 d PQ poisoning groups ( n =8 per group). A rat model of PQ poisoning was established by a single gavage of 20 wt...
December 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
Stephen D Hammack, Campbell D Carter, Aaron W Skiba, Christopher A Fugger, Josef J Felver, Joseph D Miller, James R Gord, Tonghun Lee
Planar laser-induced fluorescence (PLIF) of hydroxyl (OH) and formaldehyde (CH2 O) radicals was performed alongside stereo particle image velocimetry (PIV) at a 20 kHz repetition rate in a highly turbulent Bunsen flame. A dual-pulse burst-mode laser generated envelopes of 532 nm pulse pairs for PIV as well as a pair of 355 nm pulses, the first of which was used for CH2 O PLIF. A diode-pumped solid-state Nd:YAG/dye laser system produced the excitation beam for the OH PLIF. The combined diagnostics produced simultaneous, temporally resolved two-dimensional fields of OH and CH2 O and two-dimensional, three-component velocity fields, facilitating the observation of the interaction of fluid dynamics with flame fronts and preheat layers...
March 1, 2018: Optics Letters
Guangjing Chen, Jianquan Kan
In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed. RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32...
March 2018: Journal of Food Science and Technology
Erin J Viere, Ashley E Kuhn, Margaret H Roeder, Nicholas A Piro, W Scott Kassel, Timothy J Dudley, Jared J Paul
Attaining high oxidation states at the metal center of transition metal complexes is a key design principle for many catalytic processes. One way to support high oxidation state chemistry is to utilize ligands that are electron-donating in nature. Understanding the structural and electronic changes of metal complexes as higher oxidation states are reached is critical towards designing more robust catalysts that are able to turn over at high rates without decomposing. To this end, we report herein the changes in structural and electronic properties as [Ru(bpy)2 (44'bpy(OH)2 )]2+ is oxidized to [Ru(bpy)2 (44'bpy(OH)2 )]3+ (bpy = 2,2'-bipyridine; 44'bpy(OH)2 = 4,4'-dihydroxy-2,2'-bipyridine)...
February 23, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Xueqin Wang, Huahua Yu, Ronge Xing, Xiaolin Chen, Song Liu, Pengcheng Li
Mackerel ( Pneumatophorus japonicus ) viscera contain large amount of protein. We used five proteases to hydrolyze the viscera, and the hydrolysate treated by neutrase exhibited the highest nitrogen recovery (NR). Then we optimized the preparation conditions for mackerel viscera hydrolysate (MVH) by response surface methodology and investigated the antioxidant activity of MVH. The optimal conditions were as follows: enzyme concentration of 1,762.87 U/g, pH of 6.76, temperature of 43.75 °C, extraction time of 6...
2018: PeerJ
Idaira Pacheco-Fernández, Verónica Pino, Jacob Lorenzo-Morales, Juan H Ayala, Ana M Afonso
A novel ionic liquid (IL)-based microextraction method has been developed for the determination of four hydroxylated polycyclic aromatic hydrocarbons (OHPAHs) in urine samples. The water soluble IL-based surfactant selected as extraction solvent is decylguanidinium chloride (C10 Gu-Cl), the cytotoxicity and micellar behavior of which were evaluated. The proposed salt-induced IL-based preconcentration method simply consists in adding NaClO4 to the aqueous medium containing the IL to promote its water insolubility...
February 22, 2018: Analytical and Bioanalytical Chemistry
Constanza Mellado, Toribio Figueroa, Ricardo Baez, Rosario Castillo, Manuel Francisco Melendrez, Berta Schulz, Katherina Fernandez
The graphene aerogels' potential for use as both a hemostatic agent and dermal delivery system has scarcely been investigated. In this study, we used a sol-gel process for generating dry and stable composite aerogels based on graphene oxide (GO) and polyvinyl alcohol (PVA). Furthermore, we incorporated natural extract of País grape seed (SD) and skin (SK), rich in Proanthocyanidins (PAs or condensed tannins). The effect of the incorporation of the grape extracts was investigated in relation to the aerogels' structure, coagulation performance and the release of the extracts...
February 20, 2018: ACS Applied Materials & Interfaces
Peter Kämpfer, P D Rekha, Hans-Jürgen Busse, A B Arun, P Priyanka, Stefanie P Glaeser
A Gram-stain-negative, aerobic, non-endospore-forming organism, isolated from the rhizosphere sand of a coastal sand dune plant was studied for its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain YU-PRIM-29T was grouped within the genus Halomonas and was most closely related to Halomonas johnsoniae (97.5 %). The 16S rRNA gene sequence similarity to other Halomonas species was <97.5 %. Strain YU-PRIM-29T grew optimally at 28 °C (growth range, 10-36 °C), at a pH of 7-9 (growth range, pH 5...
February 12, 2018: International Journal of Systematic and Evolutionary Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"