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https://www.readbyqxmd.com/read/28712268/the-anti-inflammatory-and-antioxidant-properties-of-1-2-decanediol-and-willow-bark-extract-in-lipopolysaccharide-stimulated-keratinocytes
#1
Roberta DI Caprio, Giuseppe Monfrecola, Anna Balato, Nicola Balato, Franco Gasparri, Raffaella Micillo, Serena Lembo
BACKGROUND: The 1,2-decanediol (S-Mal) is an organic compound belonging to the 1,2- alkanediol family, with two hydroxyl groups located on the first and second carbon of the alkane chain, probably responsible for the enhanced anti-bacterial efficacy. The willow bark total extract (W-Mal) has been used since thousands of years as an herbal remedy for its antipyretic, analgesic, anti-inflammatory and anti-microbial activities. S-Mal is used in cosmetic preparations, whether W- Mal can be topically or systemically administered...
July 13, 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/28710634/obtaining-of-11%C3%AE-hydroxyandrost-4-ene-3-17-dione-from-natural-sterols
#2
Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina V Donova
Two-step one-pot microbial transformation enables obtaining of valuable steroids that are difficult to produce chemically. Here we describe a method for obtaining 11α-hydroxyandrost-4-ene-3,17-dione (11α-HAD) from cheap and available natural sterols (phytosterols or cholesterol).11α-HAD is a primary adrenal steroid in mammals and also a key precursor in the syntheses of halogenated corticoids. Conventional routes for its obtaining are based on chemical synthesis, or microbial hydroxylation of androst-4-ene-3,17-dione (AD)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28690684/4-fluoroprolines-conformational-analysis-and-effects-on-the-stability-and-folding-of-peptides-and-proteins
#3
Robert W Newberry, Ronald T Raines
Proline is unique among proteinogenic amino acids because a pyrrolidine ring links its amino group to its side chain. This heterocycle constrains the conformations of the main chain and thus templates particular secondary structures. Proline residues undergo post-translational modification at the 4-position to yield 4-hydroxyproline, which is especially prevalent in collagen. Interest in characterizing the effects of this modification led to the use of 4-fluoroprolines to enhance inductive properties relative to the hydroxyl group of 4-hydroxyproline and to eliminate contributions from hydrogen bonding...
2017: Topics in Heterocyclic Chemistry
https://www.readbyqxmd.com/read/28673998/insight-into-the-mechanism-of-nonenzymatic-rna-primer-extension-from-the-structure-of-an-rna-gpppg-complex
#4
Wen Zhang, Chun Pong Tam, Travis Walton, Albert C Fahrenbach, Gabriel Birrane, Jack W Szostak
The nonenzymatic copying of RNA templates with imidazole-activated nucleotides is a well-studied model for the emergence of RNA self-replication during the origin of life. We have recently discovered that this reaction can proceed through the formation of an imidazolium-bridged dinucleotide intermediate that reacts rapidly with the primer. To gain insight into the relationship between the structure of this intermediate and its reactivity, we cocrystallized an RNA primer-template complex with a close analog of the intermediate, the triphosphate-bridged guanosine dinucleotide GpppG, and solved a high-resolution X-ray structure of the complex...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28659518/the-decolorization-and-mineralization-of-orange-ii-by-microwave-assisted-ball-milling
#5
Zan Zhou, Ding Chen
This study proposed an integrated technique of reduction coupled with an oxidation process in order to acquire simultaneously both decolorization and mineralization of orange II under the condition of microwave-assisted milling. Experimental variables of initial dye concentration, iron dosage, microwave power, solution pH and initial H2O2 concentration were systematically studied. Under the optimal operational parameters (100 mg/L aqueous solution of pH 3 containing 400 mg/L H2O2 while controlling microwave power at 400 W), the results showed that the decolorization efficiency is up to 91% after reaction for 2 min and the total organic carbon removal efficiencies were 72...
June 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28653829/development-and-characterization-of-a-hydroxyl-sulfonamide-analogue-5-chloro-n-2-4-hydroxysulfamoyl-phenyl-ethyl-2-methoxy-benzamide-as-a-novel-nlrp3-inflammasome-inhibitor-for-potential-treatment-of-multiple-sclerosis
#6
Chunqing Guo, Jacob W Fulp, Yuqi Jiang, Xia Li, Jeremy E Chojnacki, Jingde Wu, Xiang-Yang Wang, Shijun Zhang
In our efforts to develop novel small-molecule inhibitors for the NOD-like receptor family pyrin-domain-containing 3 (NLRP3) inflammasome as potential disease-modifying agents to treat neurological disorders including multiple sclerosis (MS), a hydroxyl sulfonamide analogue JC-171 has been rationally designed and biologically characterized both in vitro and in vivo. Our studies established that JC-171 dose dependently inhibited LPS/ATP-induced interleukin-1β (IL-1β) release from J774A.1 macrophages with an IC50 of 8...
July 13, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28651035/material-efficient-microfluidic-platform-for-exploratory-studies-of-visible-light-photoredox-catalysis
#7
Connor W Coley, Milad Abolhasani, Hongkun Lin, Klavs F Jensen
We present an automated microfluidic platform for in-flow studies of visible-light photoredox catalysis in liquid or gas-liquid reactions at the 15 μL scale. An oscillatory flow strategy enables flexible residence time while preserving the mixing and heat transfer advantages of flow systems. The adjustable photon flux possible with the platform is characterized using actinometry. Case studies of oxidative hydroxylation of phenylboronic acids and dimerization of thiophenol demonstrate the capabilities and advantages of the system...
June 26, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28642370/in-vitro-cytochrome-p450-46a1-cyp46a1-activation-by-neuroactive-compounds
#8
Natalia Mast, Kyle W Anderson, Kevin M Johnson, Thanh T N Phan, F Peter Guengerich, Irina A Pikuleva
Cytochrome P450 46A1 (CYP46A1, cholesterol 24-hydroxylase) is the enzyme responsible for the majority of cholesterol elimination from the brain. Previously, we found that the anti-HIV drug efavirenz (EFV) can pharmacologically activate CYP46A1 in mice. Herein, we investigated whether CYP46A1 could also be activated by endogenous compounds, including major neurotransmitters. In vitro experiments with purified recombinant CYP46A1 indicated that CYP46A1 is activated by L-glutamate (L-Glu), L-aspartate, γ-aminobutyric acid, and acetylcholine, with L-Glu eliciting the highest increase (3-fold) in CYP46A1-mediated cholesterol 24-hydroxylation...
June 22, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28622467/h2o-adsorption-on-wo3-and-wo3-x-001-surfaces
#9
Elisa Albanese, Cristiana Di Valentin, Gianfranco Pacchioni
The nature of the interaction of water with the WO3 surface is of crucial importance for the use of this semiconductor oxide in photocatalysis. In this work, we investigate water adsorption and dissociation on both clean and O-deficient (001) WO3 surfaces by means of an accurate DFT approach. The O vacancy formation energy (computed with respect to O2) has been evaluated for all possible surface configurations, and the removal of the terminal O atom along the c axis is found to be preferred, costing about half the corresponding energy in the bulk...
July 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28618346/structural-analysis-of-photocrosslinkable-methacryloyl-modified-protein-derivatives
#10
Kan Yue, Xiuyu Li, Karsten Schrobback, Amir Sheikhi, Nasim Annabi, Jeroen Leijten, Weijia Zhang, Yu Shrike Zhang, Dietmar W Hutmacher, Travis J Klein, Ali Khademhosseini
Biochemically modified proteins have attracted significant attention due to their widespread applications as biomaterials. For instance, chemically modified gelatin derivatives have been widely explored to develop hydrogels for tissue engineering and regenerative medicine applications. Among the reported methods, modification of gelatin with methacrylic anhydride (MA) stands out as a convenient and efficient strategy to introduce functional groups and form hydrogels via photopolymerization. Combining light-activation of modified gelatin with soft lithography has enabled the materialization of microfabricated hydrogels...
September 2017: Biomaterials
https://www.readbyqxmd.com/read/28597389/enhanced-fenton-like-degradation-of-tce-in-sand-suspensions-with-magnetite-by-nta-edta-at-circumneutral-ph
#11
Na Wang, Daqing Jia, Yaoyao Jin, Sheng-Peng Sun, Qiang Ke
The present study investigated the degradation of trichloroethylene (TCE) in sand suspensions by Fenton-like reaction with magnetite (Fe3O4) in the presence of various chelators at circumneutral pH. The results showed that ethylenediaminetetraacetic acid (EDTA) and nitrilotriacetic acid (NTA) greatly improved the rate of TCE degradation, while [S,S]-ethylenediaminedisuccinic acid (s,s-EDDS), malonate, citrate, and phytic acid (IP6) have minimal effects on TCE degradation. Quenching tests suggested that TCE was mainly degraded by hydroxyl radical (HO(·)) attack, with about 90% inhibition on TCE degradation by the addition of HO(·) scavenger 2-propanol...
June 8, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28593636/biosynthesis-of-1%C3%AE-hydroxycorticosterone-in-the-winter-skate-leucoraja-ocellata-evidence-to-suggest-a-novel-steroidogenic-route
#12
J Wiens, R Ho, A K Brassinga, C A Deck, P J Walsh, R N Ben, K Mcclymont, T Charlton, A N Evans, W G Anderson
The present study explores the ability of intracellular bacteria within the renal-inter-renal tissue of the winter skate Leucoraja ocellata to metabolize steroids and contribute to the synthesis of the novel elasmobranch corticosteroid, 1α-hydroxycorticosterone (1α-OH-B). Despite the rarity of C1 hydroxylation noted in the original identification of 1α-OH-B, literature provides evidence for steroid C1 hydroxylation by micro-organisms. Eight ureolytic bacterial isolates were identified in the renal-inter-renal tissue of L...
June 7, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28587962/an-overview-on-cellulose-based-material-in-tailoring-bio-hybrid-nanostructured-photocatalysts-for-water-treatment-and-renewable-energy-applications
#13
REVIEW
Mohamad Azuwa Mohamed, Muhazri Abd Mutalib, Zul Adlan Mohd Hir, M F M Zain, Abu Bakar Mohamad, Lorna Jeffery Minggu, Nor Asikin Awang, W N W Salleh
A combination between the nanostructured photocatalyst and cellulose-based materials promotes a new functionality of cellulose towards the development of new bio-hybrid materials for various applications especially in water treatment and renewable energy. The excellent compatibility and association between nanostructured photocatalyst and cellulose-based materials was induced by bio-combability and high hydrophilicity of the cellulose components. The electron rich hydroxyl group of celluloses helps to promote superior interaction with photocatalyst...
June 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28581643/regioselective-synthesis-biological-evaluation-and-molecular-docking-of-dihydropyrimidin-4-ols-as-acetylcholinesterase-inhibitors
#14
Andreia M P W da Silva, Fabio M da Silva, Pablo A Nogara, Eduardo J M Dutra, Jonas D S Serres, Rogério A Saraiva, Bruna C Piccoli, Cláudia S Oliveira, Maria R C Schetinger, Vera M M Morsch, João B T Rocha, Helio G Bonacorso, Marcos A P Martins, Nilo Zanatta
A new series of 3,6-disubstituted 2-(methylthio)-4-(trifluoromethyl)-3,4-dihydropyrimidin-4-ols displaying methyl, phenyl, aryl, and heteroaryl groups at the 6-position; and methyl, ethyl, allyl, and phenyl groups at the 3-position of the dihydropyrimidine ring, were synthesized and evaluated in vitro for acetylcholinesterase (AChE) inhibitory activity. Seven compounds showed activity with IC50 values in the lower micromolar range. The compound 4-trifluoromethyl-6-(4-fluorophenyl)-3-methyl-2-methylthio-3,4-dihydropyrimidin-4-ol (6e) had the best inhibitory activity (IC50 2...
June 5, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28580552/ability-of-t1-lipase-to-degrade-amorphous-p-3hb-structural-and-functional-study
#15
Rauda A Mohamed, Abu Bakar Salleh, Adam Thean Chor Leow, Normi M Yahaya, Mohd Basyaruddin Abdul Rahman
An enzyme with broad substrate specificity would be an asset for industrial application. T1 lipase apparently has the same active site residues as polyhydroxyalkanoates (PHA) depolymerase. Sequences of both enzymes were studied and compared, and a conserved lipase box pentapeptide region around the nucleophilic serine was detected. The alignment of 3-D structures for both enzymes showed their active site residues were well aligned with an RMSD value of 1.981 Å despite their sequence similarity of only 53...
June 3, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28555391/improved-recovery-and-biological-activities-of-an-engineered-polyene-npp-analogue-in-pseudonocardia-autotrophica
#16
Hyung-Jin Won, Hye-Jin Kim, Jin-Young Jang, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
NPP A1 produced by Pseudonocardia autotrophica is a unique disaccharide-containing polyene macrolide. NPP A1 was reported to have higher water solubility and lower hemolytic toxicity than nystatin A1 while retaining its antifungal activity. An engineered NPP A1 analogue, NPP A2, was generated by inactivation of the nppL gene, encoding a P450 monooxygenase in P. autotrophica. The resulting compound exhibited the corresponding chemical structure of NPP A1 but lacked a C10 hydroxyl group. In this study, newly developed crystallization recovery methods for NPP A2 purification, followed by an evaluation of in vitro antifungal activity and hemolytic activity, were performed...
May 29, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28554477/the-arabidopsis-gasa10-gene-encodes-a-cell-wall-protein-strongly-expressed-in-developing-anthers-and-seeds
#17
Menelaos Trapalis, Song Feng Li, Roger W Parish
The Arabidopsis GASA10 gene encodes a GAST1-like (Gibberellic Acid-Stimulated) protein. Reporter gene analysis identified consistent expression in anthers and seeds. In anthers expression was developmentally regulated, first appearing at stage 7 of anther development and reaching a maximum at stage 11. Strongest expression was in the tapetum and developing microspores. GASA10 expression also occurred throughout the seed and in root vasculature. GASA10 was shown to be transported to the cell wall. Using GASA1 and GASA6 as positive controls, gibberellic acid was found not to induce GASA10 expression in Arabidopsis suspension cells...
July 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28552108/pistacia-chinensis-strong-antioxidant-and-potent-testicular-toxicity-amelioration-agent
#18
Farah Noureen, Muhammad Rashid Khan, Naseer Ali Shah, Rahmat Ali Khan, Kiran Naz, Saadia Sattar
OBJECTIVES: To evaluate in vitro and in vivo antioxidant potency of Pistacia chinensis (P. chinensis) bark and leaves extracts along with its protective role against CCl4 induced toxicity in testis of the rat. METHODS: Various in vitro models such as DPPH, ABTS, hydrogen peroxide, superoxide, hydroxyl and nitric oxide scavenging activities, anti-lipid peroxidation activity, phospho-molybdenum activity, β carotene bleaching assay was used for analysis of antioxidant potential...
April 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28545850/renal-response-to-intravascular-volume-expansion-in-euvolemic-heart-failure-patients-with-reduced-ejection-fraction-mechanistic-insights-and-clinical-implications
#19
Petra Nijst, Frederik H Verbrugge, Pieter Martens, Matthias Dupont, W H Wilson Tang, Wilfried Mullens
BACKGROUND: Untreated and preclinical heart failure patients with reduced ejection fraction (HFrEF) have an impaired ability to alleviate excess intravascular volume. OBJECTIVES: To investigate 1) the renal response to intravascular volume expansion in euvolemic and optimally treated HFrEF patients and 2) loop diuretic efficiency. METHODS: 14 healthy and 28 HFrEF patients underwent intravascular volume expansion with 1l hydroxyl ethyl starch 6% during 3h after which a loop diuretic was administered...
May 14, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28541047/multitargeted-flavonoid-inhibition-of-the-pathogenic-bacterium-staphylococcus-aureus-a-proteomic-characterization
#20
Wael A Elmasri, Rui Zhu, Wenjing Peng, Moustafa Al-Hariri, Firas Kobeissy, Phat Tran, Abdul N Hamood, Mohamed F Hegazy, Paul W Paré, Yehia Mechref
Growth inhibition of the pathogen Staphylococcus aureus with currently available antibiotics is problematic in part due to bacterial biofilm protection. Although recently characterized natural products, including 3',4',5-trihydroxy-6,7-dimethoxy-flavone [1], 3',4',5,6,7-pentahydroxy-flavone [2], and 5-hydroxy-4',7-dimethoxy-flavone [3], exhibit both antibiotic and biofilm inhibitory activities, the mode of action of such hydroxylated flavonoids with respect to S. aureus inhibition is yet to be characterized...
May 25, 2017: Journal of Proteome Research
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