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https://www.readbyqxmd.com/read/28445205/inhibition-of-microrna-429-in-the-renal-medulla-increased-salt-sensitivity-of-blood-pressure-in-sprague-dawley-rats
#1
Qing Zhu, Junping Hu, Lei Wang, Weili Wang, Zhengchao Wang, Pin-Lan Li, Krishna M Boini, Ningjun Li
BACKGROUND: We have previously shown that high salt intake suppresses the expression of prolyl hydroxylase domain-containing protein 2 (PHD2), an enzyme promoting the degradation of hypoxia-inducible factor (HIF)-1α, and increases HIF-1α along with its target genes in the renal medulla, which promotes sodium excretion and regulate salt sensitivity of blood pressure. However, it remains unknown how high salt inhibits the expression of PHD2. METHOD AND RESULTS: The current study first revealed that high-salt-induced PHD2 inhibition was due to the enhanced decay of mRNA...
April 25, 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28444822/triplex-dna-nanostructures-from-basic-properties-to-applications
#2
Yuwei Hu, Alessandro Cecconello, Andrea Idili, Francesco Ricci, Itamar Willner
Triplex nucleic acids attract recent interest as part of the rich "tool-box" of structures used to develop DNA-based nanostructures and materials. This review addresses the use of DNA triplexes to assemble sensing platforms and molecular switches. Furthermore, the pH-induced, switchable assembly and dissociation of triplex DNA-bridged nanostructures are presented. Specifically, the aggregation/deaggregation of nanoparticles and the reversible oligomerization of "origami" tiles or DNA circles are described. Also, the use of triplex-DNA structures as functional units to assemble pH-responsive systems and materials are presented...
April 26, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28444798/multi-gel-casting-apparatus-for-vertical-polyacrylamide-gels-with-in-built-solution-flow-system-and-liquid-level-detectors
#3
Praveen Maurye, Arpita Basu, Tapas Kumar Bandyopadhyay, Jayanta Kumar Biswas, Bimal Prasana Mohanty
Polyacrylamide gel electrophoresis (PAGE) is the most widely used technique for the separation and biochemical analysis of biomolecules. The ever growing field of proteomics and genomics necessitates the analysis of many proteins and nucleic acid samples to understand further about the structure and function of cells. Simultaneous analysis of multiple protein samples often requires casting of many PAGE gels. Several variants of multi-gel casting/electrophoresis apparatuses are frequently used in research laboratories...
April 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28444618/rna-dna-co-analysis-on-aged-bloodstains-from-adhesive-tapes-used-for-gunshot-residue-collection-from-hands
#4
Melanie Grabmüller, Christian Schyma, Burkhard Madea, Tim Eichhorst, Cornelius Courts
In cases of firearm related fatalities a systematic investigation at the scene of death is indispensable to differentiate between self-inflicted and homicidal gunshot injuries. A common method to preserve gunshot residues (GSR) is their collection using adhesive tapes. However, the biological material gathered at the same time by the tapes would be of special interest if backspatter, ejected from the entrance wound against the direction of fire, could be detected. In the present study we examined the success rate of co-analysis of RNA and DNA recovered from biological traces sampled with adhesive tapes...
April 25, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28444340/ligpargen-web-server-an-automatic-opls-aa-parameter-generator-for-organic-ligands
#5
Leela S Dodda, Israel Cabeza de Vaca, Julian Tirado-Rives, William L Jorgensen
The accurate calculation of protein/nucleic acid-ligand interactions or condensed phase properties by force field-based methods require a precise description of the energetics of intermolecular interactions. Despite the progress made in force fields, small molecule parameterization remains an open problem due to the magnitude of the chemical space; the most critical issue is the estimation of a balanced set of atomic charges with the ability to reproduce experimental properties. The LigParGen web server provides an intuitive interface for generating OPLS-AA/1...
April 21, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28443888/sugar-peptidic-bond-interactions-spectroscopic-characterization-of-a-model-system
#6
Ander Camiruaga, Imanol Usabiaga, Aran Insausti, Iker León, José A Fernández
Sugars are small carbohydrates which play numerous roles in living organisms such as storage of energy or as structural components. Modifications of specific sites within the glycan chain can modulate a carbohydrate's overall biological function as it happens with nucleic acids and proteins. Hence, identifying discrete carbohydrate modifications and understanding their biological effects is essential. A study of such processes requires of a deep knowledge of the interaction mechanism at the molecular level...
April 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28442904/anti-fgfr1-aptamer-tagged-superparamagnetic-conjugates-for-anticancer-hyperthermia-therapy
#7
Przemysław M Jurek, Konrad Zabłocki, Urszula Waśko, Maciej P Mazurek, Jacek Otlewski, Filip Jeleń
Compounds that recognize and strongly bind to molecular targets are one of the cornerstones of modern pharmaceutics. Work has been ongoing for the past 25 years on the therapeutic use of aptamers, nucleic acid molecules, whose three-dimensional structure is the result of interactions between complementary base pairs. The aptamers selection methods allow the oligonucleotides which bind the molecular target in its native environment to be quickly isolated from a large library of random oligonucleotides. The possibilities presented for aptamers in the field of targeted therapy require the application of effective carriers to counter the renal clearance effect and/or functional cargo to exert therapeutic action if the aptamer is only used as a targeting moiety...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28442744/biomimetic-antimicrobial-cloak-by-graphene-oxide-agar-hydrogel
#8
Massimiliano Papi, Valentina Palmieri, Francesca Bugli, Marco De Spirito, Maurizio Sanguinetti, Carlotta Ciancico, Maria Chiara Braidotti, Silvia Gentilini, Luca Angelani, Claudio Conti
Antibacterial surfaces have an enormous economic and social impact on the worldwide technological fight against diseases. However, bacteria develop resistance and coatings are often not uniform and not stable in time. The challenge is finding an antibacterial coating that is biocompatible, cost-effective, not toxic, and spreadable over large and irregular surfaces. Here we demonstrate an antibacterial cloak by laser printing of graphene oxide hydrogels mimicking the Cancer Pagurus carapace. We observe up to 90% reduction of bacteria cells...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28442309/a-universal-colorimetry-for-nucleic-acids-and-aptamer-specific-ligands-detection-based-on-dna-hybridization-amplification
#9
Shuang Li, Xinxin Shang, Jia Liu, Yujie Wang, Yingshu Guo, Jinmao You
We present a universal amplified-colorimetric for detecting nucleic acid targets or aptamer-specific ligand targets based on gold nanoparticle-DNA (GNP-DNA) hybridization chain reaction (HCR). The universal arrays consisted of capture probe and hairpin DNA-GNP. First, capture probe recognized target specificity and released the initiator sequence. Then dispersed hairpin DNA modified GNPs were cross-linked to form aggregates through HCR events triggered by initiator sequence. As the aggregates accumulate, a significant red-to purple color change can be easily visualized by the naked eye...
April 22, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28441861/anionic-polymer-and-quantum-dot-excipients-to-facilitate-sirna-release-and-self-reporting-of-disassembly-in-stimuli-responsive-nanocarrier-formulations
#10
Chad T Greco, Jason C Andrechak, Thomas H Epps Iii, Millicent O Sullivan
The incorporation of anionic excipients into polyplexes is a promising strategy for modulating siRNA binding vs. release and integrating diagnostic capabilities; however, specific design criteria and structure-function relationships are needed to facilitate the development of nanocarrier-based theranostics. Herein, we incorporated poly(acrylic acid) (PAA) and quantum dot (QD) excipients into photolabile siRNA polyplexes to increase gene silencing efficiencies by up to 100% and enable self-reporting of nanocarrier disassembly...
April 25, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28441740/a-miniature-aerosol-sensor-for-detecting-polydisperse-airborne-ultrafine-particles
#11
Chao Zhang, Dingqu Wang, Rong Zhu, Wenming Yang, Peng Jiang
Counting and sizing of polydisperse airborne nanoparticles have attracted most attentions owing to increasing widespread presence of airborne engineered nanoparticles or ultrafine particles. Here we report a miniature aerosol sensor to detect particle size distribution of polydisperse ultrafine particles based on ion diffusion charging and electrical detection. The aerosol sensor comprises a couple of planar electrodes printed on two circuit boards assembled in parallel, where charging, precipitation and measurement sections are integrated into one chip, which can detect aerosol particle size in of 30-500 nm, number concentration in range of 5 × 10²-10⁷ /cm³...
April 22, 2017: Sensors
https://www.readbyqxmd.com/read/28440819/amphiphilic-carbon-dots-as-versatile-vectors-for-nucleic-acid-and-drug-delivery
#12
Hai-Jiao Wang, Xi He, Tian-Ying Luo, Ji Zhang, Yan-Hong Liu, Xiao-Qi Yu
Carbon dot (CD)-based multifunctional delivery systems have shown great potential in both drug/gene delivery and bio-imaging. In this work, we present a strategy to simply construct amphiphilic CDs (ACDs) by conjugating hydrophobic alkyl epoxide to the surface amino groups of PEI 600-derived CDs. ACDs could well dissolve in water or organic solvents and emit bright fluorescence both in solutions and cells. (1)HNMR also suggested that ACDs may form micelle-like structures in water, and their CMC could be determined...
April 25, 2017: Nanoscale
https://www.readbyqxmd.com/read/28440732/expression-of-microrna-let-7a-positively-correlates-with-hepatitis-b-virus-replication-in-hepatocellular-carcinoma-tissues
#13
Dongni Qiu, Jian Chen, Jie Liu, Zhongguang Luo, Weiru Jiang, Jianping Huang, Zhibing Qiu, Wenjie Yue, Lijun Wu
Let-7a miRNA is downregulated in various cancers. However, in hepatocellular carcinoma (HCC) patients infected with hepatitis B virus (HBV), the relationship between let-7a and HBV replication has not been fully elucidated. Liver specimens were collected from 23 HCC patients with chronically active HBV. The serum levels of the HBV antigens hepatitis B surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg), and the HBV antibodies, anti-HBs, anti-HBe and anti-hepatitis B core antigen (anti-HBc) were measured using the microparticle enzyme immunoassay...
May 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28440726/expression-of-c-type-lectin-receptor-mrna-in-chronic-otitis-media-with-cholesteatoma
#14
Sang Hoon Kim, Seung-Ho Han, Jae Yong Byun, Moon Suh Park, Young Il Kim, Seung Geun Yeo
CONCLUSIONS: The levels of expression of various C-type lectin receptors (CLRs) messenger ribo nucleic acids (mRNAs) were significantly higher in cholesteatomas than in normal skin, suggesting that these CLRs may be involved in the pathogenesis of cholesteatoma. OBJECTIVES: Altered expression of pattern recognition receptors may be associated with immune responses in patients with cholesteatoma. This study assessed the levels of expression of CLR mRNAs in normal skin and in cholesteatoma...
June 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/28440640/nucleic-acid-nanocapsules-for-enzyme-triggered-drug-release
#15
Joseph K Awino, Saketh Gudipati, Alyssa K Hartmann, Joshua J Santiana, Dominic F Cairns-Gibson, Nicole Gomez, Jessica L Rouge
Herein we describe a nucleic acid functionalized nanocapsule in which nucleic acid ligands are assembled and disassembled in the presence of enzymes. The particles are fully degradable in response to esterases due to an embedded ester cross-linker in the particle's core. During synthesis the nanocapsules can be loaded with hydrophobic small molecules and post self-assembly undergo covalent cross-linking using copper catalyzed click chemistry. They can then be functionalized with thiolated DNA through stepwise thiolyne chemistry using UV light irradiation...
April 25, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28439837/detection-of-micrornas-using-chip-based-quantstudio-3d-digital-pcr
#16
Cristina Borzi, Linda Calzolari, Davide Conte, Gabriella Sozzi, Orazio Fortunato
Digital PCR (dPCR) is an innovative approach for detection and quantification of nucleic acid that offers an alternative method to conventional real-time quantitative PCR for absolute quantification. dPCR is a highly precise and sensitive technique that does not require a standard reference, making it a suitable method for the detection of microRNAs. The potential of these small noncoding RNA as biomarkers is on the rise, especially due to their presence in body fluids, making them easily accessible. Nevertheless, the problem of lack of consensus regarding an optimal method for miRNAs normalization compromises their use...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28439827/droplet-microfluidic-device-fabrication-and-use-for-isothermal-amplification-and-detection-of-microrna
#17
Maria Chiara Giuffrida, Roberta D'Agata, Giuseppe Spoto
Droplet microfluidics combined with the isothermal circular strand displacement polymerization (ICSDP) represents a powerful new technique to detect both single-stranded DNA and microRNA sequences. The method here described helps in overcoming some drawbacks of the lately introduced droplet polymerase chain reaction (PCR) amplification when implemented in microfluidic devices. The method also allows the detection of nanoliter droplets of nucleic acids sequences solutions, with a particular attention to microRNA sequences that are detected at the picomolar level...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28439822/improved-denaturation-of-small-rna-duplexes-and-its-application-for-northern-blotting
#18
C Jake Harris, David C Baulcombe, Attila Molnar
Small RNAs (sRNAs) are short (18-30 nucleotide) noncoding RNA molecules, which control gene expression and pathogen response in eukaryotes. They are associated with and guide nucleases to target nucleic acids by nucleotide base pairing. We found that current techniques for small RNA detection are adversely affected by the presence of complementary RNA. Thus we established FDF-PAGE (fully denaturing formaldehyde polyacrylamide gel electrophoresis), which dramatically improves denaturation efficiency and subsequently the detection of sequestered sRNAs...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28439503/circulating-cell-free-nucleic-acids-as-potential-biomarkers-for-sarcopenia-a-step-toward-personalized-medicine
#19
EDITORIAL
Gita Shafiee, Ramin Heshmat, Bagher Larijani
Sarcopenia is an age-related loss of muscle mass and function, leading to disability, morbidity and increased mortality in older people. Given the relatively high prevalence and related- outcome of the disease, correct diagnosis, screening, monitoring and treatment of sarcopenia are needed in clinical practice. Recent researches have focused on cell-free nucleic acids, which are released into the circulation following cell death, as a potential biomarker of aging and systematic inflammation. It seems that the diagnosis and treatment of sarcopenia can be possible by the help of the analysis of cell-free nucleic acids as noninvasive method...
2017: Journal of Diabetes and Metabolic Disorders
https://www.readbyqxmd.com/read/28439417/cell-free-and-intracellular-nucleic-acids-new-non-invasive-biomarkers-to-explore-male-infertility
#20
REVIEW
Anne Boissière, Anna Gala, Alice Ferrières-Hoa, Tiffany Mullet, Solenne Baillet, Amaël Petiton, Antoine Torre, Samir Hamamah
Male infertility is a devastating problem that affects many couples worldwide. However, the molecular mechanisms and causes of idiopathic male infertility remain unclear. Circulating cell-free nucleic acids have an important role in human physiology and emerging evidence suggests that they play a role in male infertility. This review summarizes recent results on cell-free and intracellular nucleic acids in male infertility and discusses their potential use as biomarkers of male infertility in the clinical practice...
2017: Basic and Clinical Andrology
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