keyword
MENU ▼
Read by QxMD icon Read
search

nucleic acids

keyword
https://www.readbyqxmd.com/read/29334465/design-of-mini-nucleic-acid-probe-for-cooperative-binding-of-rna-repeated-transcript-associated-with-myotonic-dystrophy-type-1
#1
Wei-Che Hsieh, Raman Bahal, Shivaji Thadke, Kirti Bhatt, Krzysztof Sobczak, Charles A Thornton, Danith H Ly
Toxic RNAs containing expanded trinucleotide repeats are the cause of many neuromuscular disorders, one being myotonic dystrophy type 1 (DM1). DM1 is triggered by CTG-repeat expansion in the 3'-UTR of the DMPK gene, resulting in toxic-gain of RNA function through sequestration of MBNL1 protein, among others. Herein we report the development of a relatively short MPPNA probe, two triplet-repeats in length, containing terminal pyrene moieties, that is capable of binding rCUG-repeats in a sequence-specific and selective manner...
January 15, 2018: Biochemistry
https://www.readbyqxmd.com/read/29333658/in-situ-shrna-synthesis-on-dna-polylactide-nanoparticles-to-treat-multidrug-resistant-breast-cancer
#2
Qianqian Ni, Fuwu Zhang, Yunlei Zhang, Guizhi Zhu, Zhe Wang, Zhaogang Teng, Chunyan Wang, Bryant C Yung, Gang Niu, Guangming Lu, Longjiang Zhang, Xiaoyuan Chen
Nanomedicine has shown unprecedented potential for cancer theranostics. Nucleic acid (e.g., DNA and RNA) nanomedicines are of particular interest for combination therapy with chemotherapeutics. However, current nanotechnologies to construct such nucleic acid nanomedicines, which rely on chemical conjugation or physical complexation of nucleic acids with chemotherapeutics, have restrained their clinical translation due to limitations such as low drug loading efficiency and poor biostability. Herein, in situ rolling circle transcription (RCT) is applied to synthesize short hairpin RNA (shRNA) on amphiphilic DNA-polylactide (PLA) micelles...
January 15, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29333409/human-parvovirus-b19-and-parvovirus-4-among-iranian-patients-with-hemophilia
#3
Davod Javanmard, Masood Ziaee, Hadi Ghaffari, Mohammad Hasan Namaei, Ahmad Tavakoli, Hamidreza Mollaei, Mohsen Moghoofei, Helya Sadat Mortazavi, Seyed Hamidreza Monavari
Background: Human parvovirus B19 (B19V) is one of the smallest DNA viruses and shows great resistance to most disinfectants. Therefore, it is one of the common contaminant pathogens present in blood and plasma products. Parvovirus 4 (PARV4) is a newly identified parvovirus, which is also prevalent in parenteral transmission. In this study, we aimed to evaluate the prevalence of B19V and PARV4 DNA among patients with hemophilia in Birjand County in eastern Iran. Methods: This was a cross-sectional epidemiological study comprising nearly all people with hemophilia in this region...
December 2017: Blood Research
https://www.readbyqxmd.com/read/29332992/experiment-design-for-early-molecular-events-in-hiv-infection
#4
Aditya Jagarapu, LaMont Cannon, Ryan Zurakowski
The recent introduction of integrase inhibitors to the HIV antiviral repertoire permits us to create in vitro experiments that reliably terminate HIV infection at the point of chromosomal integration. This allows us to isolate the dynamics of a single round of infection, without needing to account for the influence of multiple overlapping rounds of infection. By measuring the various nucleic acid concentrations in a population of infected target cells at multiple time points, we can infer the rates of these molecular events with great accuracy, which allows us to compare the rates between target cells with different functional phenotypes...
May 2017: Proceedings of the ... American Control Conference
https://www.readbyqxmd.com/read/29332810/sensitive-fluorescence-sensing-of-t4-polynucleotide-kinase-activity-and-inhibition-based-on-dna-polydopamine-nanospheres-platform
#5
Yao Cen, Wen-Jing Deng, Ru-Qin Yu, Xia Chu
5'-Polynucleotide kinase (PNK) is a crucial enzyme that catalyzes the phosphorylation of nucleic acid with 5'-OH termini and this phosphorylation reaction has been involved in many important cellular activities. The evaluation of PNK activity has received an increasing attention due to the significance of PNK. Here, the polydopamine nanospheres (PDANS) could adsorb single-stranded DNA (ssDNA) through π-π stacking or hydrogen bonding between nucleobases and aromatic groups of PDANS, while the interaction between double-stranded DNA (dsDNA) with PDANS was weakened due to the changed conformation...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332809/peroxidase-catalyzed-chemiluminescence-system-and-its-application-in-immunoassay
#6
REVIEW
Zhao Zhang, Jiahui Lai, Kesen Wu, Xingcan Huang, Shuai Guo, Lili Zhang, Jian Liu
Peroxidases are widely used as catalysts in chemiluminescence (CL) reaction because of their excellent catalytic activity and various selectable species, such as horseradish peroxidase (HRP), sweet potato peroxidase (SPP) and soybean peroxidase (SbP). They have been employed in many different CL systems for the determination of hydrogen peroxidase (H2O2), nucleic acid, protein and so on. In this paper, the application of peroxidases in the most commonly used luminol-H2O2 CL system was reviewed from two aspects of horseradish peroxidase (HRP) and some anionic peroxidases...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332654/evaluation-of-loop-mediated-isothermal-amplification-lamp-assay-in-the-diagnosis-of-tubercular-lymphadenitis-a-pilot-study
#7
Baijayantimala Mishra, Vinaykumar Hallur, Bijayini Behera, C Preetam, Priti Nanda Mishra, Jyotirmayee Turuk, Prasanta Raghab Mohapatra
Tubercular lymphadenitis (TBLA) contributes to 30-40% of extrapulmonary TB cases in the immunocompetent individuals and 40-50% in people with HIV. Current diagnostic methods for TBLA like Gene-Xpert or PCR are costly and conventional methods like fine needle aspiration cytology, histopathology lack sensitivity and specificity. Culture which is considered as gold standard require high turnaround time. Loop mediated isothermal amplification (LAMP) assay has been developed as a novel technique for nucleic acid amplification and has shown promising results in the diagnosis of pulmonary tuberculosis...
January 2018: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/29332574/biomacromolecular-based-fibers-in-nanomedicine-a-combination-of-drug-delivery-and-tissue-engineering
#8
Elham Arkan, Abbas Hemati Azandaryani, Pouran Moradipour, Leila Behbood
BACKGROUND: Biopolymers based materials (polysaccharides, lipids, proteins, and nucleic acids) are one of the basic resources in bio-engineering sciences because of desirable features. Moreover, nanobiotechnology innovates nanomaterial and associated technique in nano medicine (drug delivery and tissue engineering). METHODS: In the nano-medicine, fibers are introduced as a successful biomimetic extracellular matrix scaffolds and drug carrier systems. Electrospinning as a simple and cost-effective technique is used to design nanofibers...
January 12, 2018: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/29332296/plant-cell-cultures-as-model-systems-to-study-programmed-cell-death
#9
Sara Cimini, Maria Beatrice Ronci, Elisabetta Barizza, Maria Concetta de Pinto, Vittoria Locato, Fiorella Lo Schiavo, Laura De Gara
The study of programmed cell death (PCD) activated in a certain group of cells is complex when analyzed in the whole plant. Plant cell suspension cultures are useful when investigating PCD triggered by environmental and developmental stimuli. Due to their homogeneity and the possibility to synchronize their responses induced by external stimuli, these cultures are used for studying the signaling pathways leading to PCD. The first problem in the analysis of PCD in cell cultures is the quantification of cell viability/death over time...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29331743/preliminary-investigation-of-human-exhaled-breath-for-tuberculosis-diagnosis-by-multidimensional-gas-chromatography-time-of-flight-mass-spectrometry-and-machine-learning
#10
Marco Beccaria, Theodore R Mellors, Jacky S Petion, Christiaan A Rees, Mavra Nasir, Hannah K Systrom, Jean W Sairistil, Marc-Antoine Jean-Juste, Vanessa Rivera, Kerline Lavoile, Patrice Severe, Jean W Pape, Peter F Wright, Jane E Hill
Tuberculosis (TB) remains a global public health malady that claims almost 1.8 million lives annually. Diagnosis of TB represents perhaps one of the most challenging aspects of tuberculosis control. Gold standards for diagnosis of active TB (culture and nucleic acid amplification) are sputum-dependent, however, in up to a third of TB cases, an adequate biological sputum sample is not readily available. The analysis of exhaled breath, as an alternative to sputum-dependent tests, has the potential to provide a simple, fast, and non-invasive, and ready-available diagnostic service that could positively change TB detection...
January 4, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29331649/friend-or-foe-reactive-oxygen-species-production-scavenging-and-signaling-in-plant-response-to-environmental-stresses
#11
Weronika Czarnocka, Stanisław Karpiński
In the natural environment, plants are exposed to a variety of biotic and abiotic stress conditions that trigger rapid changes in the production and scavenging of reactive oxygen species (ROS). The production and scavenging of ROS is compartmentalized, which means that, depending on stimuli type, they can be generated and eliminated in different cellular compartments such as the apoplast, plasma membrane, chloroplasts, mitochondria, peroxisomes, and endoplasmic reticulum. Although the accumulation of ROS is generally harmful to cells, ROS play an important role in signaling pathways that regulate acclimatory and defense responses in plants, such as systemic acquired acclimation (SAA) and systemic acquired resistance (SAR)...
January 10, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29330974/nucleic-acid-amplification-test-for-detection-of-west-nile-virus-infection-in-pakistani-blood-donors
#12
Saifullah Khan Niazi, Maqbool Alam, Muhammad Sajid Yazdani, Eijaz Ghani, Muhammad Ali Rathore
BACKGROUND: The study was planned to determine the presence of West Nile Virus (WNV) infection in Pakistani blood donors, using Nucleic Acid Amplification Test (NAT). METHODS: The blood donors for study were selected on the basis of the standard questionnaire and routine screening results. Six donors were pooled using an automated pipettor and NAT for WNV was performed on Roche Cobas s 201 NAT system. The reactive pools were resolved in Individual Donation-NAT (ID-NAT) format and a sample from FFP bags of reactive donations was retrieved...
October 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/29330531/low-density-neutrophils-ldn-in-postoperative-abdominal-cavity-assist-the-peritoneal-recurrence-through-the-production-of-neutrophil-extracellular-traps-nets
#13
Rihito Kanamaru, Hideyuki Ohzawa, Hideyo Miyato, Shiro Matsumoto, Hidenori Haruta, Kentaro Kurashina, Shin Saito, Yoshinori Hosoya, Hironori Yamaguchi, Hiroharu Yamashita, Yasuyuki Seto, Alan Kawarai Lefor, Naohiro Sata, Joji Kitayama
Many types of immune cells appear in peritoneal cavity after abdominal surgery. In patients who underwent laparotomy due to gastric cancer, peritoneal lavages were obtained before and after surgical procedure. Cells were recovered from intermediate layer after Ficoll-Hypaque centrifugation and analyzed for phenotypes and functions, especially focused on low density neutrophils (LDN). The number of CD66b (+) LDN with mature phenotype was markedly elevated in postoperative as compared with preoperative lavages...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330463/effective-photo-enhancement-of-cellular-activity-of-fluorophore-octaarginine-antisense-pna-conjugates-correlates-with-singlet-oxygen-formation-endosomal-escape-and-chromophore-lipophilicity
#14
Reza Yarani, Takehiko Shiraishi, Peter E Nielsen
Photochemical internalization (PCI) is a cellular drug delivery method based on the generation of light-induced reactive oxygen species (ROS) causing damage to the endosomal membrane and thereby resulting in drug release to the cytoplasm. In our study a series of antisense fluorophore octaarginine peptide nucleic acid (PNA) conjugates were investigated in terms of PCI assisted cellular activity. It is found that tetramethylrhodamine and Alexa Fluor 555 conjugated octaarginine PNA upon irradiation exhibit more than ten-fold increase in antisense activity in the HeLa pLuc705 luciferase splice correction assay...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330319/correction-a-plant-derived-nucleic-acid-reconciles-type-i-ifn-and-a-pyroptotic-like-event-in-immunity-against-respiratory-viruses
#15
Dacquin M Kasumba, Takara Hajake, Seong-Wook Oh, Sergei V Kotenko, Hiroki Kato, Takashi Fujita
No abstract text is available yet for this article.
January 12, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29330189/targeted-synthesis-and-characterization-of-a-gene-cluster-encoding-nad-p-h-dependent-3%C3%AE-3%C3%AE-and-12%C3%AE-hydroxysteroid-dehydrogenases-from-eggerthella-cag-298-a-gut-metagenomic-sequence
#16
Sean M Mythen, Saravanan Devendran, Celia Méndez-García, Isaac Cann, Jason M Ridlon
Gut metagenomic sequences provide a rich source of microbial genes, the majority of which are annotated by homology or unknown. Genes and gene pathways that encode enzymes catalyzing biotransformation of host bile acids are important to identify in gut metagenomic sequences due to the importance of bile acids on gut microbiome structure and host physiology. Hydroxysteroid dehydrogenases (HSDH) are pyridine nucleotide-dependent enzymes with stereo-specificity and regio-specificity for bile acid and steroid hydroxyl groups...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29329746/evaluation-of-a-transtympanic-delivery-system-in-mus-musculus-for-extended-release-steroids
#17
Nathan H Dormer, Jennifer Nelson-Brantley, Hinrich Staecker, Cory J Berkland
OBJECTIVE: The current investigation evaluated a novel extended release delivery system for treating inner ear diseases. The platform technology consists of a film forming agent (FFA) and microsphere component to localize and extend drug delivery within the ear. STUDY DESIGN: Studies evaluated dissolution kinetics of microspheres with multiple encapsulates, testing of a variety of FFAs, and ability to localize to the round window membrane in mice in vivo. SETTING: Studies were completed at Orbis Biosciences and The University of Kansas Medical Center...
January 9, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29329181/what-is-the-optimal-time-to-retest-patients-with-a-urogenital-chlamydia-infection-a-randomized-controlled-trial
#18
Jannie J van der Helm, Rik H Koekenbier, Martijn S van Rooijen, Maarten F Schim van der Loeff, Henry J C de Vries
BACKGROUND: Chlamydia trachomatis is a common, often recurring sexually transmitted infection, with serious adverse outcomes in women. Current guidelines recommend retesting after a chlamydia infection, but the optimum timing is unknown. We assessed the optimal retest interval after urogenital chlamydia treatment. METHODS: A randomized controlled trial among urogenital chlamydia nucleic acid amplification test positive heterosexual clients of the Amsterdam sexually transmitted infection clinic...
February 2018: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/29329179/should-asymptomatic-men-who-have-sex-with-men-be-screened-for-oropharyngeal-chlamydia-clinical-outcomes-from-a-cross-sectional-study
#19
Jason J Ong, Eric P F Chow, Vesna De Petra, Deborah Williamson, Irene Pelatosis, Ben Howden, Lei Zhang, Marcus Y Chen, Catriona S Bradshaw, Jane Hocking, Christopher K Fairley
OBJECTIVE: To help inform screening guidelines, we estimated the proportion of asymptomatic men who have sex with men (MSM) with oropharyngeal chlamydia. STUDY DESIGN: An audit of asymptomatic MSM attending a sexual health service from March 2015 to April 2016 was conducted. They each had an oropharyngeal swab that was tested for Chlamydia trachomatis by transcription-mediated nucleic acid amplification. In addition, a random sample of 17 swabs that initially tested positive had confirmatory testing to determine the likelihood of true positivity...
February 2018: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/29329177/a-comparison-of-real-time-polymerase-chain-reaction-assays-for-the-detection-of-antimicrobial-resistance-markers-and-sequence-typing-from-clinical-nucleic-acid-amplification-test-samples-and-matched-neisseria-gonorrhoeae-culture
#20
Shelley W Peterson, Irene Martin, Walter Demczuk, Linda Hoang, John Wylie, Brigitte Lefebvre, Annie-Claude Labbé, Prenilla Naidu, David Haldane, Michael R Mulvey
Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance-associated mutations were tested on Neisseria gonorrhoeae-positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. N. gonorrhoeae multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 N. gonorrhoeae-negative specimens showed false-positive results...
February 2018: Sexually Transmitted Diseases
keyword
keyword
22731
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"