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https://www.readbyqxmd.com/read/28611491/phytases-of-probiotic-bacteria-characteristics-and-beneficial-aspects
#1
REVIEW
P Priyodip, P Y Prakash, S Balaji
Probiotics play a vital role in clinical applications for the treatment of diarrhea, obesity and urinary tract infections. Phytate, an anti-nutrient, chelates essential minerals that are vital for human health. In the past few decades, research reports emphasize extensively on phytate degradation in animals. There is a growing need for finding alternate strategies of phytate utilization in human, as they are unable to produce phytase. At this juncture, probiotics can be utilized for phytase production to combat mineral deficiency in humans...
June 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28603982/analysis-of-lysophospholipid-content-in-low-phytate-rice-mutants
#2
Chuan Tong, Yaling Chen, Yuanyuan Tan, Lei Liu, Daniel L E Waters, Terry Rose, Qing-Yao Shu, Jinsong Bao
As a fundamental component of nucleic acids, phospholipids and adenosine triphosphate, phosphorus (P) is critical to all life forms. However, the molecular mechanism of P translocation and distribution in rice grains are still not understood. Here, with the use of five different low phytic acids (lpa) rice mutants, the redistribution in the main P-contained compounds in rice grain, i.e. phytic acid (PA), lysophospholipid (LPL) and inorganic P (Pi) were investigated. The lpa mutants showed a significant decrease in PA and phytate-phosphorus (PA-P) concentration with the concomitant increase in Pi concentration...
June 12, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28603613/solid-state-fermentation-of-corn-soybean-meal-mixed-feed-with-bacillus-subtilis-and-enterococcus-faecium-for-degrading-antinutritional-factors-and-enhancing-nutritional-value
#3
Changyou Shi, Yu Zhang, Zeqing Lu, Yizhen Wang
BACKGROUND: Corn and soybean meal (SBM) are two of the most common feed ingredients used in pig feeds. However, a variety of antinutritional factors (ANFs) present in corn and SBM can interfere with the bioavailability of nutrients and have negative health effects on the pigs. In the present study, two-stage fermentation using Bacillus subtilis followed by Enterococcus faecium was carried out to degrade ANFs and improve the nutritional quality of corn and SBM mixed feed. Furthermore, the microbial composition and in vitro nutrient digestibility of inoculated mixed feed were determined and compared those of the uninoculated controls...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28600824/preparation-of-68ga-mg-ca-phytate-colloid-and-its-evaluation-as-a-liver-imaging-agent
#4
Chris Tsopelas, William Hsieh
The objective of this study was to investigate the radiosynthesis of 68Ga-Mg-Ca-phytate colloid, and then characterise the formulation for radiochemical purity (RCP), radioactive particle size distribution, and biodistribution in normal rats. This radiocolloid was prepared by mixing an aqueous solution of phytic acid, 68Ga3+ ions, a dispersant, Mg2+ and Ca2+ ions, and then heating the contents at 100oC for 5 minutes. After cooling the vial to 5oC, the solution was basified to pH 5 and stored in the cold. The resulting product contained 92±3% RCP 68Ga-colloidal particles and a low level (8±3%) of soluble 68Ga-Mg-Ca-phytate...
June 10, 2017: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/28572957/effect-of-spices-formulations-on-the-physicochemical-and-sensory-properties-of-nnam-gon-a-cameroonian-dish-prepared-with-cucurbitaceae-seeds
#5
Josiane E Manejo Djiogue, Richard M Nguimbou, Leopold N Tatsadjieu, Yannick D Mang, Nicolas Y Njintang
Nnam gon, a cake made by steam cooking a mixture of Cucurbitaceae seeds paste and others ingredients specially spices, is a highly prized dish in Central African region. A preliminary investigation conducted as part of this study highlighted that formulations used in the processing of Nnam gon vary according to the spices used. This study was carried out to determine the best formulation for the preparation of this dish. For this purpose, Nnam gon samples were produced from four formulations which differ according to the number of spices used: F0 (no spices); F1 (0...
May 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28554270/dephosphorylation-of-myo-inositol-phosphates-in-the-in-vitro-intestinal-caco-2-cell-model
#6
Karlis Briviba, Margit Schollenberger, Markus Rodehutscord, Ralf Greiner
Plant and microbial phytases present in raw materials can cause a dephosphorylation of phytate (myo-inositol hexakisphosphate) (InsP6)) during food processing resulting in a broad range of different myo-inositol phosphates such as pentakisphosphate (InsP5) and tetrakisphosphate (InsP4) in foods. Here, we investigated whether the human intestinal epithelium is able to dephosphorylate myo-inositol phosphates (InsP6, InsP5-, InsP4-, InsP3-isomers) using an in vitro model with differentiated human Caco-2 cells cultured on semipermeable inserts...
May 30, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28554146/improved-quinoa-growth-physiological-response-and-seed-nutritional-quality-in-three-soils-having-different-stresses-by-the-application-of-acidified-biochar-and-compost
#7
Pia Muhammad Adnan Ramzani, Lin Shan, Shazia Anjum, Waqas-Ud-Din Khan, Hu Ronggui, Muhammad Iqbal, Zaheer Abbas Virk, Salma Kausar
Quinoa (Chenopodium quinoa Willd.) is a traditional Andean agronomical resilient seed crop having immense significance in terms of high nutritional qualities and its tolerance against various abiotic stresses. However, finite work has been executed to evaluate the growth, physiological, chemical, biochemical, antioxidant properties, and mineral nutrients bioavailability of quinoa under abiotic stresses. Depending on the consistency in the stability of pH, intended rate of S was selected from four rates (0.1, 0...
May 10, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28549814/microbial-phytase-impact-of-advances-in-genetic-engineering-in-revolutionizing-its-properties-and-applications
#8
REVIEW
Mrudula Vasudevan Ushasree, Krishna Shyam, Jalaja Vidya, Ashok Pandey
Phytases are enzymes that increase the availability of phosphorous in monogastric diet and reduces the anti-nutrition effect of phytate. This review highlights contributions of recombinant technology to phytase research during the last decade with specific emphasis on new generation phytases. Application of modern molecular tools and genetic engineering have aided the discovery of novel phytase genes, facilitated its commercial production and expanded its applications. In future, by adopting most recent gene improvement techniques, more efficient next generation phytases can be developed for specific applications...
May 15, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28530567/using-combined-optimization-gc-ms-and-analytical-technique-to-analyze-the-germination-effect-on-phenolics-dietary-fibers-minerals-and-gaba-contents-of-kodo-millet-paspalum-scrobiculatum
#9
Seema Sharma, Dharmesh C Saxena, Charanjit S Riar
A central composite rotatable design was applied to study the effects of soaking time, germination time and temperature on the responses; total phenolics, total flavonoids and antioxidant activity for the biochemical enhancement of bioactive components of Kodo millet. The optimum conditions for producing germinated Kodo millet flour of highest TPC (83.01mgGAE/100g), TFC (87.53mgRUE/g) and AoxA (91.34%), were soaking time (13.81h), germination temperature (38.75°C) and germination time (35.82h). Protein increased significantly form, 6...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28527994/purification-and-characterization-of-a-protease-resistant-phytase-of-aspergillus-oryzae-sbs50-whose-properties-make-it-exceptionally-useful-as-a-feed-supplement
#10
Sapna, Bijender Singh
An extracellular phytase of Aspergillus oryzae SBS50 was purified to homogeneity using ammonium sulphate precipitation, ion-exchange and gel filtration chromatography. Purified phytase has a monomeric molecular mass of ∼80kDa exhibiting its optimal activity at pH 5.0 and 50°C with a T 1/2 of 300min at 50°C. Phytase of A. oryzae displayed broad substrate specificity with Vmax and Km values of 58.82μmol/ml/min and 1.14mM, respectively, for calcium phytate. Purity and homogeneity of the phytase was confirmed by high performance liquid chromatography and MALDI-TOF analysis revealed the identification of a peptide showing homology with acid phosphatase of Aspergillus oryzae RIB40...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28507005/perspective-what-makes-it-so-difficult-to-mitigate-worldwide-anemia-prevalence
#11
Klaus Schümann, Noel W Solomons
Anemia can be related to decreased production or increased loss of erythrocytes, or both, leading to many underlying and often overlapping causes. A largely cereal-based diet with plenty of phytates, polyphenols, and other ligands that inhibit intestinal iron absorption predominated in preindustrial Europe and predominates in present-day developing countries alike. In both situations, we find poor hygienic conditions, which frequently lead to anemia of inflammation. The large number of possible causes and their interaction shows why it is so difficult to mitigate anemia prevalence...
May 2017: Advances in Nutrition
https://www.readbyqxmd.com/read/28504139/the-protective-role-of-phytate-in-the-oxidative-degradation-of-cereal-beta-glucans
#12
Yu-Jie Wang, Ruzhen Zhan, Tuula Sontag-Strohm, Ndegwa Henry Maina
This study investigated the role of phytate in the Fenton reaction induced oxidative degradation of cereal β-glucan. Viscosity analysis showed that the degradation rate was high in the beginning of oxidation, which fitted to the second order kinetic model. Oat β-glucan contained significant amount of residual phytate and after the residual phytate was removed, faster degradation was shown compared to the original oat β-glucan. Adding the same amount of phytic acid (PA) to the phytate removed β-glucan sample also retarded the degradation but not as efficiently as the residual phytate...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28503185/phosphate-uptake-from-phytate-due-to-hyphae-mediated-phytase-activity-by-arbuscular-mycorrhizal-maize
#13
Xin-Xin Wang, Ellis Hoffland, Gu Feng, Thomas W Kuyper
Phytate is the most abundant form of soil organic phosphorus (P). Increased P nutrition of arbuscular mycorrhizal plants derived from phytate has been repeatedly reported. Earlier studies assessed acid phosphatase rather than phytase as an indication of mycorrhizal fungi-mediated phytate use. We investigated the effect of mycorrhizal hyphae-mediated phytase activity on P uptake by maize. Two maize (Zea mays L.) cultivars, non-inoculated or inoculated with the arbuscular mycorrhizal fungi Funneliformis mosseae or Claroideoglomus etunicatum, were grown for 45 days in two-compartment rhizoboxes, containing a root compartment and a hyphal compartment...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28482677/subsequent-bone-and-metabolic-responses-of-broilers-to-high-non-phytate-phosphorus-diets-in-the-starter-period
#14
N Baradaran, M H Shahir, Z Asadi Kermani
1. An experiment was performed to elucidate the subsequent effects of high-non-phytate phosphorus (NPP) diets on growth performance, blood metabolites, bone characteristics and P retention of broilers fed on low-NPP grower diets. The 42-d study was designed as a 2 × 2 × 2 + 1 factorial, which included two starter NPP concentrations (4.5 and 5.5 g/kg; d 0-21), two grower NPP concentrations (1.5 and 2.3 g/kg; d 22-42), with or without phytase (1000 FTU/kg), with a reference diet containing an adequate NPP concentration over the course of the trial...
May 31, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28482506/determination-of-copper-ions-using-a-phytic-acid-polypyrrole-nanowires-modified-glassy-carbon-electrode
#15
Nan Wang, Hongxiu Dai, Donglei Wang, Houyi Ma, Meng Lin
This study reports a sensitive electro-analytical method for the determination of trace amounts of Cu(2+) using a phytate functionalized polypyrrole nanowires (PPy NWs) modified glass carbon electrode. The phytic acid/polypyrrole (PA/PPy) NWs were prepared by an electrostatic adsorption and ultrasonic mixing. The results showed that both PPy NWs and PA/PPy NWs modified glassy carbon electrodes have electrochemical responses toward Cu(2+). However, owing to the synergistic contribution between the PPy NWs and PA, the PA/PPy NWs modified electrode exhibited higher sensitivity than that of PPy NWs modified electrode...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28469549/repeat-24-hour-recalls-and-locally-developed-food-composition-databases-a-feasible-method-to-estimate-dietary-adequacy-in-a-multi-site-preconception-maternal-nutrition-rct
#16
Rebecca L Lander, K Michael Hambidge, Nancy F Krebs, Jamie E Westcott, Ana Garces, Lester Figueroa, Gabriela Tejeda, Adrien Lokangaka, Tshilenge S Diba, Manjunath S Somannavar, Ranjitha Honnayya, Sumera A Ali, Umber S Khan, Elizabeth M McClure, Vanessa R Thorsten, Kristen B Stolka
Background: Our aim was to utilize a feasible quantitative methodology to estimate the dietary adequacy of >900 first-trimester pregnant women in poor rural areas of the Democratic Republic of the Congo, Guatemala, India and Pakistan. This paper outlines the dietary methods used. Methods: Local nutritionists were trained at the sites by the lead study nutritionist and received ongoing mentoring throughout the study. Training topics focused on the standardized conduct of repeat multiple-pass 24-hr dietary recalls, including interview techniques, estimation of portion sizes, and construction of a unique site-specific food composition database (FCDB)...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28460975/zinc-bioavailability-from-whey-enthalpy-entropy-compensation-in-protein-binding
#17
Ning Tang, Leif H Skibsted
Zinc amino acid or peptide complexes improve zinc absorption, however, the thermodynamics behind the interaction between zinc ion and these potential ligands is not well characterized. Therefore, binding of zinc to amino acids, peptides and whey proteins were investigated by isothermal titration calorimetry to provide useful information for improving zinc bioavailability which is lowered by many food components like phytate. Zinc binding to lactoferrin and to bovine serum albumin was found exothermic with ∆H=-100kJ/mol and -30kJ/mol, respectively, in aqueous 0...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28460943/ancestral-andean-grain-quinoa-as-source-of-lactic-acid-bacteria-capable-to-degrade-phytate-and-produce-b-group-vitamins
#18
Silvana L Carrizo, Cecilia E Montes de Oca, Jonathan E Laiño, Nadia E Suarez, Graciela Vignolo, Jean Guy LeBlanc, Graciela Rollán
The lactic acid bacteria (LAB) microbiota of quinoa grains (QG) and spontaneous sourdough (QSS) was evaluated. Different strains of Lactobacillus (L.) plantarum (7), L. rhamnosus (5), L. sakei (1), Pediococcus (Ped.) pentosaceus (9), Leuconostoc (Leuc.) mesenteroides (1), Enterococcus (E.) casseliflavus (2), E. mundtii (3), E. hirae (1), E. gallinarum (12), Enterococcus sp. (1), and E. hermanniensis (2) were isolated, identified and characterized. Only four strains isolated from QSS and eight strains isolated from QG showed amylolytic activity...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28447381/land-use-influences-phosphatase-gene-microdiversity-in-soils
#19
Andrew L Neal, Maike Rossmann, Charles Brearley, Elsy Akkari, Cervin Guyomar, Ian M Clark, Elisa Allen, Penny R Hirsch
Phosphorus cycling exerts significant influence upon soil fertility and productivity - processes largely controlled by microbial activity. We adopted phenotypic and metagenomic approaches to investigate phosphatase genes within soils. Microbial communities in bare fallowed soil showed a marked capacity to utilise phytate for growth compared to arable or grassland soil communities. Bare fallowed soil contained lowest concentrations of orthophosphate. Analysis of metagenomes indicated phoA, phoD and phoX, and histidine acid and cysteine phytase genes were most abundant in grassland soil which contained the greatest amount of NaOH-EDTA extractable orthophosphate...
April 26, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28432964/phytate-induced-arsenic-uptake-and-plant-growth-in-arsenic-hyperaccumulator-pteris-vittata
#20
Xue Liu, Jing-Wei Fu, Ni Tang, E B da Silva, Yue Cao, Benjamin L Turner, Yanshan Chen, Lena Q Ma
Phytate is abundant in soils, which is stable and unavailable for plant uptake. However, it occurs in root exudates of As-hyperaccumulator Pteris vittata (PV). To elucidate its effect on As uptake and growth, P. vittata were grown on agar media (63 μM P) containing 50 μM As and/or 50 or 500 μM phytate with non As-hyperaccumulator Pteris ensiformis (PE) as a congeneric control for 60 d. Phytate induced efficient As and P uptake, and enhanced growth in PV, but had little effects on PE. The As concentrations in PV fronds and roots were 157 and 31 mg kg(-1) in As50+phytate50, 2...
April 19, 2017: Environmental Pollution
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