keyword
MENU ▼
Read by QxMD icon Read
search

Phytate

keyword
https://www.readbyqxmd.com/read/29145111/a-novel-protein-tyrosine-phosphatase-like-phytase-from-lactobacillus-fermentum-nkn51-cloning-characterization-and-application-in-mineral-release-for-food-technology-applications
#1
Rekha Sharma, Piyush Kumar, Vandana Kaushal, Rahul Das, Naveen Kumar Navani
A novel protein tyrosine phosphatase like phytase (PTPLP), designated as PhyLf from probiotic bacterium Lactobacillus fermentum NKN51 was identified, cloned, expressed and characterized. The recombinant PhyLf showed specific activity of 174.5 U/mg. PhyLf exhibited strict specificity towards phytate and optimum temperature at 60 °C, pH 5.0 and ionic strength of 100 mM. Km and Kcat of PhyLf for phytate were 0.773 mM and 84.31 s(-1), respectively. PhyLf exhibited high resistance against oxidative inactivation...
November 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29123461/intestinal-absorption-and-factors-influencing-bioavailability-of-magnesium-an-update
#2
REVIEW
Jan Philipp Schuchardt, Andreas Hahn
Background: Information on the bioavailability of the essential mineral Mg2+ is sparse. Objective/Method: Evaluation of the present knowledge on factors influencing the bioavailability and intestinal absorption of Mg2+. Results: Mg2+ is absorbed via a paracellular passive and a transcellular active pathway that involves TRPM6/7 channel proteins. The bioavailability of Mg2+ varies within a broad range, depending on the dose, the food matrix, and enhancing and inhibiting factors...
November 2017: Current Nutrition and Food Science
https://www.readbyqxmd.com/read/29112750/high-stocking-density-alters-bone-related-calcium-and-phosphorus-metabolism-by-changing-intestinal-absorption-in-broiler-chickens
#3
Z W Sun, Q H Fan, X X Wang, Y M Guo, H J Wang, X Dong
Live performance, bone health and metabolic responses to the interaction among stocking density and dietary concentrations of total calcium (TCa) and non-phytate phosphorus (NPP) were determined on 2,232 Ross 308 female broilers over a 3-wk experimental period. From 22 d of age, birds were randomly divided into 48 groups and provided with different corn-soybean meal-based diets varying in TCa (0.70% or 0.90%) and NPP (0.28% or 0.36%) content at 1 of 2 stocking densities [28.6 (LSD, 13 broilers/m2) and 39.6 (HSD, 18 broilers/m2) kg of predicted final BW/m2 floor space], according to a 2 × 2 × 2 factorial design with 6 replications in each treatment...
November 3, 2017: Poultry Science
https://www.readbyqxmd.com/read/29109915/mechanisms-of-oat-avena-sativa-l-acclimation-to-phosphate-deficiency
#4
Ewa Żebrowska, Marta Milewska, Iwona Ciereszko
Background: Deficiency of available forms of phosphorus is common in most soils and causes reduction of crop plants growth and yield. Recently, model plants responses to phosphate (Pi) deficiency have been intensively studied. However, acclimation mechanisms of cereals like oat (Avena sativa L.), to low Pi stress remains not fully understood. Oat plants have been usually cultured on poor soils, with a low nutrient content, but their responses to such conditions are not well known, therefore the main goal of the study was to investigate the mechanisms that enable oat plants to grow under low Pi conditions...
2017: PeerJ
https://www.readbyqxmd.com/read/29095154/crystallization-and-x-ray-diffraction-analysis-of-native-and-selenomethionine-substituted-phyh-di-from-bacillus-sp-hjb17
#5
Fang Lu, Bei Zhang, Yong Liu, Ying Song, Gangxing Guo, Duo Feng, Huoqing Huang, Peilong Yang, Wei Gao, Sujuan Guo, Bin Yao
Phytases are phosphatases that hydrolyze phytates to less phosphorylated myo-inositol derivatives and inorganic phosphate. β-Propeller phytases, which are very diverse phytases with improved thermostability that are active at neutral and alkaline pH and have absolute substrate specificity, are ideal substitutes for other commercial phytases. PhyH-DI, a β-propeller phytase from Bacillus sp. HJB17, was found to act synergistically with other single-domain phytases and can increase their efficiency in the hydrolysis of phytate...
November 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29089855/solid-state-fermentation-reduces-phytic-acid-level-improves-the-profile-of-myo-inositol-phosphates-and-enhances-the-availability-of-selected-minerals-in-flaxseed-oil-cake
#6
Robert Duliński, Bożena Stodolak, Łukasz Byczyński, Aleksander Poreda, Anna Starzyńska-Janiszewska, Krzysztof Żyła
Flaxseed oil cake was subjected to fermentation with Rhizopus oligosporus (DSM 1964 and ATCC 64063), and the phytate (InsP6) content, myo-inositol phosphate profile and in vitro bioavailability of essential minerals were studied. Flaxseed oil cake had a phytate mass fraction of 13.9 mg/g. A 96-hour fermentation of flaxseed oil cake by R. oligosporus DSM 1964 and R. oligosporus ATCC 64063 decreased the InsP6 content by 48 and 33%, respectively. The strains had different phytate-degrading activities: fermentation of flaxseed oil cake with R...
September 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/29077965/phosphorus-bioavailability-in-increased-protein-reduced-fiber-canola-meal-conventional-canola-meal-and-soybean-meal-fed-to-crossbred-chicks
#7
C D Hanna, C K Foran, P L Utterback, H H Stein, C M Parsons
Three experiments were conducted to evaluate 3 increased-protein, reduced-fiber canola meals (CM) (CMA, CMB, and Test CM), 2 conventional CM (CCM), and 2 soybean meals (SBM). For determination of P bioavailability in CM and SBM, a P-deficient cornstarch-dextrose-SBM basal diet was fed as Diet 1. The latter basal diet was then supplemented with 0.05 and 0.10% P from KH2PO4 or 12.5 and 25% of a CM or SBM. In addition, the effect of phytase enzyme on bioavailability of the P in CMA, Test CM, and one of the CCM was determined using P-deficient 45% CM-cornstarch-dextrose diets (0...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29077957/effects-of-a-high-level-of-phytase-on-broiler-performance-bone-ash-phosphorus-utilization-and-phytate-dephosphorylation-to-inositol
#8
A E Gautier, C L Walk, R N Dilger
An experiment was conducted to evaluate how the addition of microbial phytase influenced growth performance, bone mineralization, tissue P content, apparent digestibility and retention, and inositol phosphate (IP) concentrations in broilers fed diets with varying mineral matrices from 2 to 23 d of age. At 2 d of age, chicks were randomly allotted to receive 1 of 6 experimental diets arranged as a 3 × 2 factorial of mineral matrix [control diet with 1.0% Ca and 0.5% non-phytate phosphorus (NPP); mineral matrix 1 with 0...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29074246/simulated-bioavailability-of-phosphorus-from-aquatic-macrophytes-and-phytoplankton-by-aqueous-suspension-and-incubation-with-alkaline-phosphatase
#9
Weiying Feng, Fengchang Wu, Zhongqi He, Fanhao Song, Yuanrong Zhu, John P Giesy, Ying Wang, Ning Qin, Chen Zhang, Haiyan Chen, Fuhong Sun
Bioavailability of phosphorus (P) in biomass of aquatic macrophytes and phytoplankton and its possible relationship with eutrophication were explored by evaluation of forms and quantities of P in aqueous extracts of dried macrophytes. Specifically, effects of hydrolysis of organically-bound P by the enzyme alkaline phosphatase were studied by use of solution (31)P-nuclear magnetic resonance (NMR) spectroscopy. Laboratory suspensions and incubations with enzymes were used to simulate natural releases of P from plant debris...
October 23, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29072359/fungal-extracellular-phosphatases-their-role-in-p-cycling-under-different-ph-and-p-sources-availability
#10
I F Della Mónica, M S Godoy, A M Godeas, J M Scervino
AIMS: The aim of this work is to analyse the effect of pH, fungal identity and P chemical nature on microbial development and phosphatase release, discussing solubilization and mineralization processes in P cycling. METHODS AND RESULTS: P solubilizing fungi (Talaromyces flavus, T. helicus L, T. helicus N, T. diversus and Penicillium purpurogenum) were grown under three pH conditions (6, 6.5 and 8.5) and with different inorganic (calcium, iron, aluminium, rock) and organic (lecithin, phytate) P sources...
October 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29053604/nutritional-and-acquired-deficiencies-in-inositol-bioavailability-correlations-with-metabolic-disorders
#11
REVIEW
Simona Dinicola, Mirko Minini, Vittorio Unfer, Roberto Verna, Alessandra Cucina, Mariano Bizzarri
Communities eating a western-like diet, rich in fat, sugar and significantly deprived of fibers, share a relevant increased risk of both metabolic and cancerous diseases. Even more remarkable is that a low-fiber diet lacks some key components-as phytates and inositols-for which a mechanistic link has been clearly established in the pathogenesis of both cancer and metabolic illness. Reduced bioavailability of inositol in living organisms could arise from reduced food supply or from metabolism deregulation. Inositol deregulation has been found in a number of conditions mechanistically and epidemiologically associated to high-glucose diets or altered glucose metabolism...
October 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29052415/activity-of-escherichia-coli-aspergillus-niger-and-rye-phytase-toward-partially-phosphorylated-myo-inositol-phosphates
#12
Ralf Greiner
Kinetic parameters for the dephosphorylation of sodium phytate and a series of partially phosphorylated myo-inositol phosphates were determined at pH 3.0 and pH 5.0 for three phytase preparations (Aspergillus niger, Escherichia coli, rye). The enzymes showed lower affinity and turnover numbers at pH 3 compared to pH 5 toward all myo-inositol phosphates included in the study. The number and distribution of phosphate groups on the myo-inositol ring affected the kinetic parameters. Representatives of the individual phytate dephosphorylation pathways were identified as the best substrates of the phytases...
October 31, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29050599/development-of-chitosan-sodium-phytate-nanoparticles-as-a-potent-antibacterial-agent
#13
Jie Yang, Hao Lu, Man Li, Jing Liu, Shuangling Zhang, Liu Xiong, Qingjie Sun
Chitosan nanoparticles have attracted considerable attention as a potential carrier for food and pharmaceutical applications. Herein, using natural sodium phytate as a gelation agent, we developed a new type of green and biocompatible chitosan nanoparticles. We discovered that the chitosan-sodium phytate nanoparticles exhibited potent antibacterial activities. The chitosan-sodium phytate nanoparticles prepared from low molecular weight (LMW, 140±7kDa) and medium molecular weight (MMW, 454±21kDa) chitosan were spherical...
December 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29036002/multimodal-imaging-in-klippel-tr%C3%A3-naunay-weber-syndrome-clinical-photography-computed-tomoangiography-infrared-thermography-and-99mtc-phytate-lymphoscintigraphy
#14
Su Wan Kim, Heesung Song
We report the case of a 19-year-old man who presented with a 12-year history of progressive fatigue, feeling hot, excessive sweating, and numbness in the left arm. He had undergone multimodal imaging and was diagnosed as having Klippel-Trénaunay-Weber syndrome (KTWS). This is a rare congenital disease, defined by combinations of nevus flammeus, venous and lymphatic malformation, and hypertrophy of the affected limbs. Lower extremities are affected mostly. Conventional modalities for evaluating KTWS are ultrasonography, CT, MRI, lymphoscintigraphy, and angiography...
October 13, 2017: Clinical Nuclear Medicine
https://www.readbyqxmd.com/read/29033683/regional-liver-disorder-with-differences-in-the-accumulation-of-99m-tc-phytate-and-99m-tc-galactosyl-human-serum-albumin
#15
Kohei Kotani, Joji Kawabe, Shigeaki Higashiyama, Susumu Shiomi
We report a 56-year-old woman with regional liver disorder due to acute hepatitis. Computed tomographic images showed low signal density at a plain phase and prolonged contrast effect at a late phase in the left hepatic lobe, in which an accumulation of (99m)Tc-phytate increased, whereas that of (99m)Tc-galactosyl human serum albumin (GSA) decreased. Meanwhile, in the right lobe, an accumulation of (99m)Tc-GSA showed more increased than that of (99m)Tc-phytate. Liver biopsy showed massive hepatocyte necrosis and interface hepatitis in the left lobe, and moderate hepatitis in the right lobe...
October 2017: World Journal of Nuclear Medicine
https://www.readbyqxmd.com/read/29017237/biochemical-characterization-of-a-psychrophilic-phytase-from-an-artificially-cultivable-morel-morchella-importuna
#16
Hao Tan, Jie Tang, Xiaolin Li, Tianhai Liu, Renyun Miao, Zhongqian Huang, Yong Wang, Bingcheng Gan, Weihong Peng
Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of histidine acidic phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified and characterized. The phytase had an optimum temperature at 25°C, which is the lowest among all the known phytases to our knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut...
October 11, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28992026/amino-acid-and-phosphorus-digestibility-of-fermented-corn-soybean-meal-mixed-feed-with-bacillus-subtilis-and-enterococcus-faecium-fed-to-pigs
#17
C Shi, Y Zhang, Y Yin, C Wang, Z Lu, F Wang, J Feng, Y Wang
In the present study, a 2-stage solid-state fermentation process using followed by was performed with the purpose of improving the nutritional quality of corn and soybean meal (SBM) mixed feed (MF). The matrix of native MF and fermented MF (FMF) was analyzed using scanning electron microscopy to determine external structures after solid-state fermentation. Results of the present study indicated that the FMF had a greater concentration of CP and total P compared with the unfermented feed, whereas the contents of β-conglycinin, glycinin, phytate P, raffinose, stachyose, and NDF in FMF declined by 77...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28991999/effect-of-combined-xylanase-and-phytase-on-growth-performance-apparent-total-tract-digestibility-and-carcass-characteristics-in-growing-pigs-fed-corn-based-diets-containing-high-fiber-coproducts
#18
Y D Jang, P Wilcock, R D Boyd, M D Lindemann
Phytate has been shown to be an antinutrient, and the feeding of high levels of phytase can break down phytate to improve nutrient utilization and pig performance. Dietary xylanase targets arabinoxylan breakdown, thereby improving energy utilization in pigs. However, the effects of simultaneous supplementation have not been clearly determined. Crossbred pigs ( = 45; mean initial weight, 26.4 ± 0.2 kg) were allotted to 1 of 9 treatments to evaluate the effects of both xylanase (endo-1,4-β xylanase [EC 3.2...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28971283/non-phytate-phosphorus-requirement-for-broilers-from-8-to-21%C3%A2-days-of-age-under-heat-stress-conditions
#19
Evandro Ferreira Cardoso, Juarez Lopes Donzele, Rita Flávia Miranda de Oliveira Donzele, Bruna Leite Sufiate, Amanda Dione Silva, Tarciso Tizziani
Two trials were conducted to determine the non-phytate phosphorus (nPP) requirement for broiler under heat stress. In both trials, birds were distributed in a completely randomized 4 × 2 factorial design with four nPP concentrations: 0.25, 0.35, 0.45, and 0.55%, and two Ca supply techniques: Ca fixed at 0.899% (CaF) or varying along with nPP aiming a 2:1 Ca to nPP ratio (CaV). Both trials had eight pens/treatment, with nine and five birds/pen for exp. 1 and exp. 2, respectively. nPP concentration had no effect on feed intake (FI), body weight gain (BWG), feed conversion ratio (FCR), nor fat deposition ratio (FDR)...
October 3, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28956139/the-effects-of-activating-almonds-on-consumer-acceptance-and-gastrointestinal-tolerance
#20
Heidi Taylor, Kirsten Webster, Andrew R Gray, Siew Ling Tey, Alex Chisholm, Karl Bailey, Shivani Kumari, Rachel C Brown
PURPOSE: Recommendations to soak nuts prior to consumption to reduce phytate concentrations and improve gastrointestinal tolerance have received much attention in the popular press. This is despite no supporting scientific evidence for the practice. There is also a lack of information about how soaking nuts might affect consumer acceptability. This study primarily assessed the effects of soaking almonds on consumer acceptance and secondly assessed effects on gastrointestinal tolerance...
September 27, 2017: European Journal of Nutrition
keyword
keyword
22727
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"