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https://www.readbyqxmd.com/read/28546002/catechins-activate-muscle-stem-cells-by-myf5-induction-and-stimulate-muscle-regeneration
#1
A Rum Kim, Kyung Min Kim, Mi Ran Byun, Jun-Ha Hwang, Jung Il Park, Ho Taek Oh, Hyo Kyeong Kim, Mi Gyeong Jeong, Eun Sook Hwang, Jeong-Ho Hong
Muscle weakness is one of the most common symptoms in aged individuals and increases risk of mortality. Thus, maintenance of muscle mass is important for inhibiting aging. In this study, we investigated the effect of catechins, polyphenol compounds in green tea, on muscle regeneration. We found that (-)-epicatechin gallate (ECG) and (-)-epigallocatechin-3-gallate (EGCG) activate satellite cells by induction of Myf5 transcription factors. For satellite cell activation, Akt kinase was significantly induced after ECG treatment and ECG-induced satellite cell activation was blocked in the presence of Akt inhibitor...
May 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28544472/engineering-muscle-cell-alignment-through-3d-bioprinting
#2
Pamela Mozetic, Sara Maria Giannitelli, Manuele Gori, Marcella Trombetta, Alberto Rainer
Processing of hydrogels represents a main challenge for the prospective application of additive manufacturing (AM) to soft tissue engineering. Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro culture required for conventional cell-seeded scaffolds seeking to fabricate constructs with tailored structural and functional properties. In this work, we present a simple AM methodology that exploits the thermoresponsive behavior of a block copolymer (Pluronic®) as a means to obtain good shape retention at physiological conditions and to induce cellular alignment...
May 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28542067/grb2-associated-binder-1-is-required-for-extrafusal-and-intrafusal-muscle-fiber-development
#3
So Y Park, So Y Jang, Yoon K Shin, Byeol A Yoon, Hye J Lee, Hwan T Park
The neuregulin-1 (NRG1) signaling pathway plays an important role in the development of the peripheral neuromuscular system, including in muscle spindle and postnatal myelination. We previously showed that NRG1 on the axonal membrane regulates peripheral nerve myelination through Grb2-associated binder 1 (Gab1), a scaffolding mediator of receptor tyrosine kinase signaling. Here, we determined the role of Gab1 in the development of muscles and the muscle spindle using muscle-specific conditional Gab1 knockout mice...
May 24, 2017: Neuroreport
https://www.readbyqxmd.com/read/28541289/mir-29b-contributes-to-multiple-types-of-muscle-atrophy
#4
Jin Li, Mun Chun Chan, Yan Yu, Yihua Bei, Ping Chen, Qiulian Zhou, Liming Cheng, Lei Chen, Olivia Ziegler, Glenn C Rowe, Saumya Das, Junjie Xiao
A number of microRNAs (miRNAs, miRs) have been shown to play a role in skeletal muscle atrophy, but their role is not completely understood. Here we show that miR-29b promotes skeletal muscle atrophy in response to different atrophic stimuli in cells and in mouse models. miR-29b promotes atrophy of myotubes differentiated from C2C12 or primary myoblasts, and conversely, its inhibition attenuates atrophy induced by dexamethasone (Dex), TNF-α and H2O2 treatment. Targeting of IGF-1 and PI3K(p85α) by miR-29b is required for induction of muscle atrophy...
May 25, 2017: Nature Communications
https://www.readbyqxmd.com/read/28539819/dexamethasone-treatment-at-the-myoblast-stage-enhanced-c2c12-myocyte-differentiation
#5
Der-Sheng Han, Wei-Shiung Yang, Tung-Wei Kao
Background: Glucocorticoids induce skeletal muscle atrophy in many clinical situations; however, their hypertrophic and pro-differentiation effects on myotubes have rarely been reported. We hypothesized that dexamethasone (DEX) has a dual effect on muscle differentiation, and aimed to develop a new differentiation protocol for C2C12 cell line. Methods: Dose- and time-dependent effect of DEX on C2C12 myoblast cell line was analyzed at myoblast and myotube stage, respectively. The level of differentiation was determined by myh1, pax7, atrogin-1, and myostatin mRNA expression and fusion index...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28536872/cytoprotective-propensity-of-green-tea-polyphenols-against-citrinin-induced-skeletal-myotube-damage-in-c2c12-cells
#6
G R Sharath Babu, N Ilaiyaraja, Farhath Khanum, T Anand
The mycotoxin citrinin, is produced by several species of Penicillium, Aspergillus and Monascus, and is capable of inducing cytotoxicity, oxidative stress and apoptosis. The aim of the present study was to investigate the effect of citrinin in mouse skeletal muscle cells (C2C12) and to overcome the cellular adverse effects by supplementing green tea extract (GTE) rich in polyphenols. C2C12 myoblasts were differentiated to myotubes and were exposed to citrinin in a dose dependent manner (0-100 µM) for 24 h and IC50 value was found to be 100 µM that resulted in decreased cell viability, increased LDH leakage and compromised membrane integrity...
May 23, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28536426/toll-like-receptor-4-mediates-lewis-lung-carcinoma-induced-muscle-wasting-via-coordinate-activation-of-protein-degradation-pathways
#7
Guohua Zhang, Zhelong Liu, Hui Ding, Hongyu Miao, Jose M Garcia, Yi-Ping Li
Cancer-induced cachexia, characterized by muscle wasting, is a lethal metabolic syndrome with undefined etiology. Current consensus is that multiple factors contribute to cancer-induced muscle wasting, and therefore therapy requires combinational strategies. Here, we show that Toll-like receptor 4 (TLR4) mediates cancer-induced muscle wasting by directly activating muscle catabolism as well as stimulating an innate immune response in mice bearing Lewis lung carcinoma (LLC), and targeting TLR4 alone effectively abrogate muscle wasting...
May 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28531999/zro2-toughened-al2o3-based-near-net-shaped-femoral-head-unique-fabrication-approach-3d-microstructure-burst-strength-and-muscle-cell-response
#8
Debasish Sarkar, Sourav Mandal, B S Reddy, Nitu Bhaskar, D C Sundaresh, Bikramjit Basu
The development of reliable biomedical devices demands the use of an integrated manufacturing protocol with comprehensive understanding of prototype characterization in terms of 3D microstructural analysis along with biocompatibility. While addressing these issues for ZrO2 (3mol% Y2O3 stabilized)-toughened Al2O3-based femoral head prototypes, the present work reports a unique fabrication protocol involving a sequence of uniaxial compaction followed by pre-sintering, machining, final sintering and polishing to ensure dimensional tolerance with respect to the design of patient-specific femoral head...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28529450/mir-696-regulates-c2c12-cell-proliferation-and-differentiation-by-targeting-cntfr%C3%AE
#9
Han Wang, Lei Shi, Tingting Liang, BinBin Wang, WangJun Wu, Guosheng Su, Julong Wei, Pinghua Li, Ruihua Huang
Micro-696 (miR-696) has been previously known as an exercise related miRNA, which has a profound role in fatty acid oxidation and mitochondrial biogenesis of skeletal muscle. However, its role in skeletal myoblast proliferation and differentiation is still unclear. In this study, we found that miR-696 expressed highly in skeletal muscle and reduced during C2C12 myoblasts differentiation. MiR-696 overexpression repressed C2C12 myoblast proliferation and myofiber formation, while knockdown of endogenous miR-696 expression showed opposite results...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28526071/retinoid-acid-induced-microrna-31-5p-suppresses-myogenic-proliferation-and-differentiation-by-targeting-camkii%C3%AE
#10
Bo Liu, Chao Liu, Wei Cong, Nan Li, Nan Zhou, Yi Tang, Chao Wei, Han Bai, Ying Zhang, Jing Xiao
BACKGROUND: We previously reported that Wnt5a/CaMKIIδ (calcium/calmodulin-dependent protein kinase II delta) pathway was involved in the embryonic tongue deformity induced by excess retinoic acid (RA). Our latest study found that the expression of miR-31-5p, which was predicted to target the 3'UTR of CamkIIδ, was raised in the RA-treated embryonic tongue. Thus, we hypothesized that the excess RA regulated Wnt5a/CaMKIIδ pathway through miR-31-5p in embryonic tongue. METHODS: C2C12 myoblast line was employed as an in vitro model to examine the suppression of miR-31-5p on CamkIIδ expression, through which RA impaired the myoblast proliferation and differentiation in embryonic tongue...
May 11, 2017: Skeletal Muscle
https://www.readbyqxmd.com/read/28514856/cell-adhesion-on-rgd-displaying-knottins-with-varying-numbers-of-tryptophan-amino-acids-to-tune-the-affinity-for-assembly-on-cucurbit-8-uril-surfaces
#11
Shrikrishnan Sankaran, Emanuela Cavatorta, Jurriaan Huskens, Pascal Jonkheijm
Cell adhesion is studied on multivalent knottins, displaying RGD ligands with a high affinity for integrin receptors, that are assembled on CB[8]-methylviologen-modified surfaces. The multivalency in the knottins stems from the number of tryptophan amino acid moieties, between 0 and 4, that can form a heteroternary complex with cucurbit[8]uril (CB[8]) and surface-tethered methylviologen (MV(2+)). The binding affinity of the knottins with CB[8] and MV(2+) surfaces was evaluated using surface plasmon resonance spectroscopy...
May 25, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28511130/design-synthesis-and-biological-evaluation-of-uncharged-catechol-derivatives-as-selective-inhibitors-of-ptp1b
#12
Xiang-Qian Li, Qi Xu, Jiao Luo, Li-Jun Wang, Bo Jiang, Ren-Shuai Zhang, Da-Yong Shi
Protein tyrosine phosphatases 1B (PTP1B) is a promising and validated therapeutic target to effectively treat T2DM and obesity. However, the development of charged PTP1B inhibitors was restricted due to their low cell permeability and poor bioavailability. Based on active natural products, two series of uncharged catechol derivatives were identified as PTP1B inhibitors by targeting a secondary aryl phosphate-binding site as well as the catalytic site. The most potent inhibitor 22 showed an IC50 of 0.487 μM against PTP1B and strong selectivity (27-fold) over TCPTP...
May 3, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28507941/in-vitro-cytotoxicity-effects-of-197-au-pamamg4-and-198-au-pamamg4-nanocomposites-against-mcf7-and-4t1-breast-cancer-cell-lines
#13
Simin Janitabar-Darzi, Reza Rezaei, Kamal Yavari
Purpose: Study on gold based therapeutic agents for cancer cells deracination has become under scrutiny in recent years owing to effective treatments are not available for rapidly progressive cancers. The aim of present study was to examine efficiency of radioactive (198)Au/PAMAMG4 and non-radioactive (197)Au/PAMAMG4 nancomposites against 4T1 and MCF7 breast cancer cell lines. Methods: The PAMAMG4 dendrimer was treated with the gold anions and then, the mixture was chemically reduced by NaBH4. Prepared (197)Au/PAMAMG4 was bombarded by thermal neutrons in the Tehran Research Reactor to (198)Au/PAMAMG4 be produced...
April 2017: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28506762/the-nuclear-phosphatase-scp4-regulates-foxo-transcription-factors-during-muscle-wasting-in-chronic-kidney-disease
#14
Xinyan Liu, Rizhen Yu, Lijing Sun, Giacomo Garibotto, Xia Lin, Yanlin Wang, Sandhya S Thomas, Rongshan Li, Zhaoyong Hu
Chronic kidney disease (CKD) and related inflammatory responses stimulate protein-energy wasting, a complication causing loss of muscle mass. Primarily, muscle wasting results from accelerated protein degradation via autophagic/lysosomal and proteasomal pathways, but mechanisms regulating these proteolysis pathways remain unclear. Since dephosphorylation of FoxOs regulates ubiquitin/proteasome protein metabolism, we tested whether a novel nuclear phosphatase, the small C-terminal domain phosphatase (SCP) 4, regulates FoxOs signaling and, in turn, muscle wasting...
May 12, 2017: Kidney International
https://www.readbyqxmd.com/read/28504656/effect-of-il-1%C3%AE-tnf-%C3%AE-and-igf-1-on-trans-endothelial-passage-of-synthetic-vectors-through-an-in-vitro-vascular-endothelial-barrier-of-striated-muscle
#15
J-P Gomez, C Gonçalves, C Pichon, P Midoux
When administrated in the blood circulation, plasmid DNA (pDNA) complexed with synthetic vectors must pass through a vascular endothelium to transfect underlying tissues. Under inflammatory condition, cytokines effects can modify the endothelium integrity. Here, the trans-endothelial passage (TEP) of DNA complexes including polyplexes, lipoplexes and lipopolyplexes was investigated in the presence of TNF-α-, IL-1β or IGF-1. The experiments were performed by using an in vitro model comprising a monolayer of mouse cardiac endothelial cells (MCEC) seeded on a trans-well insert and the transfection of C2C12 myoblasts cultured on the lower chamber as read out of TEP...
May 15, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28501652/a-new-adenovector-system-for-implementing-thymulin-gene-therapy-for-inflammatory-disorders
#16
María F Zappa-Villar, Micaela López-León, Joaquín Pardo, Mariana Costa, Rosana Crespo, Mireille Dardenne, Rodolfo G Goya, Paula C Reggiani
Thymulin is a thymic peptide possessing anti-inflammatory effects. In order to manipulate thymulin expression in gene therapy studies, we built a bidirectional regulatable two-vector Tet-Off system and the corresponding control system. The experimental two-vector system, ETV, consists of a recombinant adenovector (RAd) harboring an expression cassette centered on a Tet-Off bidirectional promoter flanked by a synthetic gene for thymulin and the gene for humanized Green Fluorescent Protein (hGFP). The second adenovector of this system, RAd-tTA, constitutively expresses the regulatory protein tTA...
May 11, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28498977/consequences-of-megf10-deficiency-on-myoblast-function-and-notch1-interactions
#17
Madhurima Saha, Satomi Mitsuhashi, Michael D Jones, Kelsey Manko, Hemakumar M Reddy, Christine Bruels, Kyung-Ah Cho, Christina A Pacak, Isabelle Draper, Peter B Kang
Mutations in MEGF10 cause early onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD), a rare congenital muscle disease, but the pathogenic mechanisms remain largely unknown. We demonstrate that short hairpin RNA (shRNA)-mediated knockdown of Megf10, as well as overexpression of the pathogenic human p.C774R mutation, leads to impaired proliferation and migration of C2C12 cells. Myoblasts from Megf10-/- mice and Megf10-/-/mdx double knockout (dko) mice also show impaired proliferation and migration compared to myoblasts from wild type and mdx mice, whereas the dko mice show histological abnormalities that are not observed in either single mutant mouse...
May 11, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28485800/gdf11-does-not-improve-the-palmitate-induced-insulin-resistance-in-c2c12
#18
Y-Y Jing, D Li, F Wu, L-L Gong, R Li
OBJECTIVE: GDF11 (Growth Differentiation factor 11) has been reported to rejuvenate skeletal muscle, heart and brain in aged mice, and the aged skeletal muscle is closely related to insulin resistance. We wondered whether GDF11 has an effect on skeletal muscle insulin resistance. MATERIALS AND METHODS: High fat diet induced obese mice with insulin resistance were established in vivo. Palmitate-induced insulin resistance in C2C12 myotubes was established in vitro...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28475656/nmr-based-metabolomic-analysis-for-the-effects-of-creatine-supplementation-on-mouse-myoblast-cell-line-c2c12
#19
Wenqi Xu, Donghai Lin, Caihua Huang
Creatine (Cr) supplementation has drawn much attention from researchers owing to its widespread efficacy in sports, and more recently, in therapeutic fields. However, the underlying molecular mechanisms remain elusive. Here, we performed nuclear magnetic resonance-based metabolomic analysis to address the metabolic profile of aqueous extracts from the mouse myoblast cell line C2C12 exposed to 2 mM Cr for 24 h (the Cr-treated group). Results showed that Cr supplementation facilitated the proliferation of C2C12 myoblasts...
May 5, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28474889/promotion-of-glucose-uptake-in-c2c12-myotubes-by-cereal-flavone-tricin-and-its-underlying-molecular-mechanism
#20
Sohyun Kim, Gwang-Woong Go, Jee-Young Imm
The effect of tricin, a methylated flavone widely distributed in cereals, on glucose uptake and the underlying molecular mechanism was investigated using C2C12 myotubes. Tricin significantly increased glucose uptake in C2C12 myotubes, regardless of the absence (1.4-fold at 20 μM) or presence (1.6-fold at 20 μM) of insulin. The GLUT4 expression on the plasma membrane was increased 1.6-fold after tricin treatment (20 μM) in the absence of insulin. Tricin treatment significantly activated the insulin-dependent cell signaling pathway, including the activation of insulin receptor substrate-1 (IRS1), phosphoinositide 3-kinase (PI3K), protein kinase B (AKT), and AKT substrate of 160 kDa (AS160)...
May 5, 2017: Journal of Agricultural and Food Chemistry
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