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https://www.readbyqxmd.com/read/28734256/3d-porous-polyurethanes-featured-by-different-mechanical-properties-characterization-and-interaction-with-skeletal-muscle-cells
#1
Lorenzo Vannozzi, Leonardo Ricotti, Tommaso Santaniello, Tercio Terencio, Reinier Oropesa-Nunez, Claudio Canale, Francesca Borghi, Arianna Menciassi, Cristina Lenardi, Irini Gerges
The fabrication of biomaterials for interaction with muscle cells has attracted significant interest in the last decades. However, 3D porous scaffolds featured by a relatively low stiffness (almost matching the natural muscle one) and highly stable in response to cyclic loadings are not available at present, in this context. This work describes 3D polyurethane-based porous scaffolds featured by different mechanical properties. Biomaterial stiffness was finely tuned by varying the cross-linking degree of the starting foam...
July 14, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28731188/hispidin-rescues-palmitate%C3%A2-induced-insulin-resistance-in-c2c12-myotubes
#2
Jong Seok Lee, Jun Myoung Park, Sarah Lee, Hye Jin Lee, Hee-Sun Yang, Joohong Yeo, Ki Rim Lee, Byung Hyun Choi, Eock Kee Hong
Skeletal muscle serves an important role in the utilization of glucose during insulin‑stimulated conditions. Excessive saturated fatty acids are considered to be a major contributing factor to insulin resistance in skeletal muscle cells. The present study investigated the effects of hispidin on palmitate‑induced insulin resistance in C2C12 skeletal muscle myotubes via an MTT assay, glucose uptake assay, Oil‑Red‑O staining and western blot analysis. Hispidin reversed the palmitate‑induced inhibition of glucose uptake, and inhibited palmitate‑induced intracellular lipid accumulation...
July 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28725675/comparisons-of-elisa-and-western-blot-assays-for-detection-of-autophagy-flux
#3
Sung-Hee Oh, Yong-Bok Choi, June-Hyun Kim, Conrad C Weihl, Jeong-Sun Ju
We analyzed autophagy/mitophagy flux in vitro (C2C12 myotubes) and in vivo (mouse skeletal muscle) following the treatments of autophagy inducers (starvation, rapamycin) and a mitophagy inducer (carbonyl cyanide m-chlorophenylhydrazone, CCCP) using two immunodetection methods, ELISA and Western blotting, and compared their working range, accuracy, and reliability. The ELISAs showed a broader working range than that of the LC3 Western blots (Table 1). Table 2 showed that data value distribution was tighter and the average standard error from the ELISA was much smaller than those of the Western blot, directly relating to the accuracy of the assay...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28725668/data-on-myod-reduction-by-autophagy-in-c2c12-cells
#4
Yeong-Min Yoo, Yung Chul Park
Autophagy is a highly regulated physiologic mechanism in which cells maintain homeostasis by degrading excessive or unnecessary proteins and damaged or aged organelles through the lysosomal machinery (Yorimitsu and Klionsky, 2005) [1]. MyoD is basic helix-loop-helix (bHLH) transcription factors that regulate myoblast proliferation and myogenic differentiation. MyoD is expressed in adult skeletal muscle (Megeney et al., 1996) [2] and adult fibers (Brack et al., 2005) [3]. MyoD is mainly degraded by the ubiquitin-proteasome system (Floyd et al...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28725008/methylglyoxal-and-advanced-glycation-end-products-insight-of-the-regulatory-machinery-affecting-the-myogenic-program-and-of-its-modulation-by-natural-compounds
#5
Mohammad Hassan Baig, Arif Tasleem Jan, Gulam Rabbani, Khurshid Ahmad, Jalaluddin M Ashraf, Taeyeon Kim, Han Sol Min, Yong Ho Lee, Won-Kyung Cho, Jin Yeul Ma, Eun Ju Lee, Inho Choi
Methylglyoxal (MG) is a reactive dicarbonyl intermediate and a precursor of advanced glycation end products (AGEs). The authors investigated the role played by AGEs in muscle myopathy and the amelioration of its effects by curcumin and gingerol. In addition to producing phenotypical changes, MG increased oxidative stress and reduced myotube formation in C2C12 cells. RAGE (receptor for AGEs) expression was up-regulated and MYOD and myogenin (MYOG) expressions were concomitantly down-regulated in MG-treated cells...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28715871/improvement-of-mitochondrial-function-by-celastrol-in-palmitate-treated-c2c12-myotubes-via-activation-of-pi3k-akt-signaling-pathway
#6
Mohamad Hafizi Abu Bakar, Joo Shun Tan
Compelling evidences posited that high level of saturated fatty acid gives rise to mitochondrial dysfunction and inflammation in the development of insulin resistance in skeletal muscle. Celastrol is a pentacyclic triterpenoid derived from the root extracts of Tripterygium wilfordii that possesses potent anti-inflammatory properties in a number of animal models with metabolic diseases. However, the cellular mechanistic action of celastrol in alleviating obesity-induced insulin resistance in skeletal muscle remains largely unknown...
July 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28710412/exosomes-derived-from-pancreatic-cancer-cells-induce-insulin-resistance-in-c2c12-myotube-cells-through-the-pi3k-akt-foxo1-pathway
#7
Lantian Wang, Bo Zhang, Wen Zheng, Muxing Kang, Qing Chen, Wenjie Qin, Chao Li, Yuefeng Zhang, Yingkuan Shao, Yulian Wu
Prospective epidemiological studies have consistently suggested that pancreatic cancer-associated new-onset diabetes mellitus (PC-DM) represents a potential platform for early diagnose of pancreatic cancer (PC). Despite the studies performed, the mechanism behind this phenomenon remains ambiguous. In this study, we explored the effects of two types of exosomes released by murine pancreatic cancer and ductal epithelial cells on murine skeletal muscle cells. The results show that PC-derived exosomes can readily enter C2C12 myotubes, triggering lipidosis and glucose intake inhibition...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28707279/sarcolipin-expression-is-repressed-by-endoplasmic-reticulum-stress-in-c2c12-myotubes
#8
Nobuhiko Takahashi, Atsushi P Kimura, Sumiyoshi Naito, Mika Yoshida, Osamu Kumano, Takeshi Suzuki, Satoshi Itaya, Mitsuru Moriya, Masahiro Tsuji, Masahiro Ieko
Sarcolipin is a transmembrane protein expressed in the sarco/endoplasmic reticulum of skeletal and atrial muscles in large animals. Sarcolipin plays crucial roles in heat production through modifying the function of sarco/endoplasmic reticulum Ca(2+) ATPase, thereby being involved in thermogenesis and systemic metabolism. In skeletal muscle, endoplasmic reticulum (ER) stress has been implicated in several conditions, such as insulin resistance, muscle diseases, and hypo/hyper-contraction. Here, we investigated the effect of ER stress on sarcolipin expression in skeletal muscle cells, C2C12 myotubes...
July 13, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28707095/chronic-administration-of-myristic-acid-improves-hyperglycaemia-in-the-nagoya-shibata-yasuda-mouse-model-of-congenital-type-2-diabetes
#9
Tamae Takato, Kai Iwata, Chiaki Murakami, Yuko Wada, Fumio Sakane
AIMS/HYPOTHESIS: Previously, we demonstrated that myristic acid (14:0) increases levels of diacylglycerol kinase (DGK) δ, a key enzyme involved in type 2 diabetes exacerbation, and enhances glucose uptake in C2C12 myotube cells. Moreover, results from a population-based cohort study suggest that consumption of high-fat dairy products, which contain high amounts of myristic acid, is associated with a lower risk of developing type 2 diabetes. Taken together, we hypothesised that intake of myristic acid reduces type 2 diabetes risk in vivo...
July 13, 2017: Diabetologia
https://www.readbyqxmd.com/read/28706950/effects-of-cobalt-chloride-a-hypoxia-mimetic-agent-on-autophagy-and-atrophy-in-skeletal-c2c12-myotubes
#10
Rui Chen, Ting Jiang, Yanling She, Jiehua Xu, Cheng Li, Shanyao Zhou, Huijuan Shen, Huacai Shi, Shuang Liu
BACKGROUND: Hypoxia-induced autophagy and muscle wasting occur in several environmental and pathological conditions. However, the molecular mechanisms underlying the effects of the hypoxia-mimetic agent CoCl2 on autophagy and muscle atrophy are still unclear. METHODS: C2C12 myotubes were exposed to increasing concentrations of CoCl2 for 24 hours. Quantitative RT-PCR, Western blotting, and transmission electron microscopy were performed to confirm autophagy occurs...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28704996/imidacloprid-promotes-high-fat-diet-induced-adiposity-in-female-c57bl-6j-mice-and-enhance-adipogenesis-in-3t3-l1-adipocytes-via-ampk%C3%AE-mediated-pathway
#11
Quancai Sun, Weipeng Qi, Xiao Xiao, Szu-Hao Yang, Daeyoung Kim, Kyong-Sup Yoon, John M Clark, Yeonhwa Park
Imidacloprid, a neonicotinoid insecticide, has been reported to enhance adipogenesis and resulted in insulin resistance in vitro. It was also reported to promote high fat diet-induced obesity and insulin resistance in male C57BL/6J mice. The current study was to determine the effects of imidacloprid and its interaction with dietary fat in the development of adiposity and insulin resistance in female C57BL/6J mice. Mice were fed with imidacloprid (0.06, 0.6, or 6 mg/kg bw/day) mixed in low fat (4% w/w) or high fat (20% w/w) diet for 12 weeks...
July 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28702326/rev-erb-co-regulates-muscle-regeneration-via-tethered-interaction-with-the-nf-y-cistrome
#12
Ryan D Welch, Chun Guo, Monideepa Sengupta, Katherine J Carpenter, Natalie A Stephens, Stacy A Arnett, Marvin J Meyers, Lauren M Sparks, Steven R Smith, Jinsong Zhang, Thomas P Burris, Colin A Flaveny
OBJECTIVE: The loss of skeletal muscle mass and strength are a central feature of traumatic injury and degenerative myopathies. Unfortunately, pharmacological interventions typically fail to stem the long-term decline in quality of life. Reduced Rev-Erb-mediated gene suppression in cultured C2C12 myoblasts has been shown to stimulate myoblast differentiation. Yet the mechanisms that allow Rev-Erb to pleiotropically inhibit muscle differentiation are not well understood. In this study, we sought to elucidate the role of Rev-Erb in the regulation of muscle differentiation and regeneration in vivo...
July 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28697464/laminin-111-enriched-fibrin-hydrogels-for-skeletal-muscle-regeneration
#13
Madison Marcinczyk, Hady Elmashhady, Muhamed Talovic, Andrew Dunn, Faiz Bugis, Koyal Garg
Laminin (LM)-111 supplementation has improved muscle regeneration in several models of disease and injury. This study investigated a novel hydrogel composed of fibrinogen and LM-111. Increasing LM-111 concentration (50-450 μg/mL) in fibrin hydrogels resulted in highly fibrous scaffolds with progressively thinner interlaced fibers. Rheological testing showed that all hydrogels had viscoelastic behavior and the Young's modulus ranged from 2-6KPa. C2C12 myobalsts showed a significant increase in VEGF production and decrease in IL-6 production on LM-111 enriched fibrin hydrogels as compared to pure fibrin hydrogels on day 4...
July 3, 2017: Biomaterials
https://www.readbyqxmd.com/read/28694473/5-lo-inhibition-ameliorates-palmitic-acid-induced-er-stress-oxidative-stress-and-insulin-resistance-via-ampk-activation-in-murine-myotubes
#14
Hyun Jeong Kwak, Hye-Eun Choi, Hyae Gyeong Cheon
Leukotriene B4 (LTB4) production via the 5-lipoxygenase (5-LO) pathway contributes to the development of insulin resistance in adipose and hepatic tissues, but the role of LTB4 in skeletal muscle is relatively unknown. Here, the authors investigated the role of LTB4 in C2C12 myotubes in palmitic acid (PA)-induced ER stress, inflammation and insulin resistance. PA (750 μM) evoked lipotoxicity (ER stress, oxidative stress, inflammation and insulin resistance) in association with LTB4 production. 5-LO inhibition reduced all the lipotoxic effects induced by PA...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28691106/recombinant-myostatin-reduces-highly-expressed-micrornas-in-differentiating-c2c12-cells
#15
Zachary A Graham, Rita De Gasperi, William A Bauman, Christopher P Cardozo
Myostatin is small glycopeptide that is produced and secreted by skeletal muscle. It is a potent negative regulator of muscle growth that has been associated with conditions of frailty. In C2C12 cells, myostatin limits cell differentiation. Myostatin acts through activin receptor IIB, activin receptor-like kinase (ALK) and Smad transcription factors. microRNAs (miRNA) are short, 22 base pair nucleotides that bind to the 3' UTR of target mRNA to repress translation or reduce mRNA stability. In the present study, expression in differentiating C2C12 cells of the myomiRs miR-1 and 133a were down-regulated following treatment with 1 μg of recombinant myostatin at 1 d post-induction of differentiation while all myomiRs (miR-1, 133a/b and 206) were upregulated by SB431542, a potent ALK4/5/7 inhibitor which reduces Smad2 signaling, at 1 d and all, with the exception of miR-206, were upregulated by SB431542 at 3 d...
March 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/28688716/exercise-induced-glut4-transcription-via-inactivation-of-hdac4-5-in-mouse-skeletal-muscle-in-an-ampk%C3%AE-2-dependent-manner
#16
Yanmei Niu, Tianyi Wang, Sujuan Liu, Hairui Yuan, Huige Li, Li Fu
Abnormal glucose metabolism induces various metabolic disorders such as insulin resistance and type 2 diabetes. Regular exercise improved glucose uptake and enhanced glucose oxidation by increasing GLUT4 transcription in skeletal muscle. However, the regulatory mechanisms of GLUT4 transcription in response to exercise are poorly understood. AMPK is a sensor of exercise and upstream kinase of class II HDACs that act as transcriptional repressors. We used 6-week treadmill exercise or one single-bout exercise wild type or AMPKα2(-/-) C57BL/6J mice to explore how HDACs regulate GLUT4 transcription and the underlying molecular mechanisms mediated by AMPK in the physiologic process of exercise...
July 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28675664/obestatin-controls-the-ubiquitin-proteasome-and-autophagy-lysosome-systems-in-glucocorticoid-induced-muscle-cell-atrophy
#17
Tania Cid-Díaz, Icía Santos-Zas, Jessica González-Sánchez, Uxía Gurriarán-Rodríguez, Carlos S Mosteiro, Xesús Casabiell, Tomás García-Caballero, Vincent Mouly, Yolanda Pazos, Jesús P Camiña
BACKGROUND: Many pathological states characterized by muscle atrophy are associated with an increase in circulating glucocorticoids and poor patient prognosis, making it an important target for treatment. The development of treatments for glucocorticoid-induced and wasting disorder-related skeletal muscle atrophy should be designed based on how the particular transcriptional program is orchestrated and how the balance of muscle protein synthesis and degradation is deregulated. Here, we investigated whether the obestatin/GPR39 system, an autocrine/paracrine signaling system acting on myogenesis and with anabolic effects on the skeletal muscle, could protect against glucocorticoid-induced muscle cell atrophy...
July 3, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/28675237/ursolic-acid-induces-mitochondrial-biogenesis-through-the-activation-of-ampk-and-pgc-1-in-c2c12-myotubes-a-possible-mechanism-underlying-its-beneficial-effect-on-exercise-endurance
#18
Jihang Chen, Hoi Shan Wong, Pou Kuan Leong, Hoi Yan Leung, Wing Man Chan, Kam Ming Ko
Mitochondrial biogenesis, which involves an increase in mitochondrial number and the overall capacity of oxidative phosphorylation, is a critical determinant of skeletal muscle function. Recent findings have shown that some natural products can enhance mitochondrial adaptation to aerobic exercise, which in turn improves exercise performance, presumably by delaying muscle fatigue. Ursolic acid (UA), a natural triterpene, is commonly found in various vegetables and fruits. In the current study, UA was shown to increase mitochondrial mass and ATP generation capacity, with a concomitant production of a low level of mitochondrial reactive oxygen species (ROS) in C2C12 myotubes...
July 19, 2017: Food & Function
https://www.readbyqxmd.com/read/28671759/electroactive-3d-scaffolds-based-on-silk-fibroin-and-water-borne-polyaniline-for-skeletal-muscle-tissue-engineering
#19
Mengyao Zhang, Baolin Guo
Silk fibroin (SF) with good biocompatibility and degradability has great potential for tissue engineering. However, the SF based scaffolds lack the electroactivity to regulate the myogenic differentiation for the regeneration of muscle tissue, which is sensitive to electrical signal. Herein, a series of electroactive biodegradable scaffolds based on SF and water-soluble conductive poly(aniline-co-N-(4-sulfophenyl) aniline) (PASA) via a green method for skeletal muscle tissue engineering are designed. SF/PASA scaffolds are prepared by vortex of aqueous solution of SF and PASA under physiological condition...
July 3, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28666833/ludwigia-octovalvis-extract-improves-glycemic-control-and-memory-performance-in-diabetic-mice
#20
Wei-Sheng Lin, Jung-Hsin Lo, Jo-Hsuan Yang, Hao-Wei Wang, Shou-Zen Fan, Jui-Hung Yen, Pei-Yu Wang
ETHNOPHARMACOLOGICAL RELEVANCE: Ludwigia octovalvis (Jacq.) P.H. Raven (Onagraceae) extracts have historically been consumed as a healthful drink for treating various conditions, including edema, nephritis, hypotension and diabetes. AIM OF THE STUDY: We have previously shown that Ludwigia octovalvis extract (LOE) can significantly extend lifespan and improve age-related memory deficits in Drosophila melanogaster through activating AMP-activated protein kinase (AMPK)...
June 27, 2017: Journal of Ethnopharmacology
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