keyword
MENU ▼
Read by QxMD icon Read
search

C2C12

keyword
https://www.readbyqxmd.com/read/28930190/oligonol-a-low-molecular-weight-polyphenol-derived-from-lychee-alleviates-muscle-loss-in-diabetes-by-suppressing-atrogin-1-and-murf1
#1
Hung-Wen Liu, Yen-Ju Chen, Yun-Ching Chang, Sue-Joan Chang
Stimulation of the ubiquitin-proteasome pathway-especially E3 ubiquitin ligases Atrogin-1 and MuRF1-is associated with muscle loss in diabetes. Elevated lipid metabolites impair myogenesis. Oligonol, a low molecular weight polyphenol derived from lychee, exhibited anti-diabetic and anti-obesity properties, suggesting it could be a proper supplement for attenuating muscle loss. Dietary (10 weeks) oligonol supplementation (20 or 200 mg/kg diet) on the skeletal muscle loss was investigated in diabetic db/db mice...
September 20, 2017: Nutrients
https://www.readbyqxmd.com/read/28928419/the-histone-deacetylase-sirt6-blocks-myostatin-expression-and-development-of-muscle-atrophy
#2
Sadhana A Samant, Abhinav Kanwal, Vinodkumar B Pillai, Riyue Bao, Mahesh P Gupta
Muscle wasting, also known as cachexia, is associated with many chronic diseases, which worsens prognosis of primary illness leading to enhanced mortality. Molecular basis of this metabolic syndrome is not yet completely understood. SIRT6 is a chromatin-bound member of the sirtuin family, implicated in regulating many cellular processes, ranging from metabolism, DNA repair to aging. SIRT6 knockout (SIRT6-KO) mice display loss of muscle, fat and bone density, typical characteristics of cachexia. Here we report that SIRT6 depletion in cardiac as well as skeletal muscle cells promotes myostatin (Mstn) expression...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28928090/investigation-of-antidiabetic-potential-of-phyllanthus-niruri-l-using-assays-for-%C3%AE-glucosidase-muscle-glucose-transport-liver-glucose-production-and-adipogenesis
#3
Maliheh Najari Beidokhti, Mia Vesterholm Andersen, Hoda M Eid, Mayra Luz Sanchez Villavicencio, Dan Staerk, Pierre S Haddad, Anna K Jäger
Phyllanthus niruri is used in herbal medicine for treatment of diabetes. The objective of this study was to investigate the antidiabetic potential of P. niruri, using assays for α-glucosidase, muscle glucose transport, liver glucose production and adipogenesis. α-Glucosidase inhibitory activity was performed on aqueous and ethanolic extract of aerial parts of P. niruri. The aqueous and ethanolic extract of P. niruri showed α-glucosidase inhibitory activity with IC50 values of 3.7 ± 1.1 and 6.3 ± 4...
September 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28927261/hdac11-inhibits-myoblast-differentiation-through-repression-of-myod-dependent-transcription
#4
Sang Kyung Byun, Tae Hyeon An, Min Jeong Son, Da Som Lee, Hyun Sup Kang, Eun-Woo Lee, Baek Soo Han, Won Kon Kim, Kwang-Hee Bae, Kyoung-Jin Oh, Sang Chul Lee
Abnormal differentiation of muscle is closely associated with aging (sarcopenia) and diseases such as cancer and type II diabetes. Thus, understanding the mechanisms that regulate muscle differentiation will be useful in the treatment and prevention of these conditions. Protein lysine acetylation and methylation are major post-translational modification mechanisms that regulate key cellular processes. In this study, to elucidate the relationship between myogenic differentiation and protein lysine acetylation/methylation, we performed a PCR array of enzymes related to protein lysine acetylation/ methylation during C2C12 myoblast differentiation...
September 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28919162/mir-101-1-expression-pattern-in-qinchuan-cattle-and-its-role-in-the-regulation-of-cell-differentiation
#5
Jiyao Wu, Dandan He, Binglin Yue, Chunlei Zhang, Xingtang Fang, Hong Chen
MiRNAs have emerged as key regulators of skeletal muscle development, but the knowledge of miRNAs in the molecular network of muscle development remains poorly understood. In this study, we designed to examine the biological function of bovine-miR-101-1. The bovine miR-101-1 was detected in the skeletal muscle of fetal, calf and adult cattle. Its abundance was significantly higher in the skeletal muscle of calf cattle than that in fetal and adult cattle. In the course of C2C12 myoblast differentiation, the expression of miR-101-1 gradually increased...
September 14, 2017: Gene
https://www.readbyqxmd.com/read/28918129/where-do-substrates-of-diacylglycerol-kinases-come-from-diacylglycerol-kinases-utilize-diacylglycerol-species-supplied-from-phosphatidylinositol-turnover-independent-pathways
#6
REVIEW
Fumio Sakane, Satoru Mizuno, Daisuke Takahashi, Hiromichi Sakai
Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). Mammalian DGK comprises ten isozymes (α-κ) and regulates a wide variety of physiological and pathological events, such as cancer, type II diabetes, neuronal disorders and immune responses. DG and PA consist of various molecular species that have different acyl chains at the sn-1 and sn-2 positions, and consequently, mammalian cells contain at least 50 structurally distinct DG/PA species. Because DGK is one of the components of phosphatidylinositol (PI) turnover, the generally accepted dogma is that all DGK isozymes utilize 18:0/20:4-DG derived from PI turnover...
September 9, 2017: Advances in Biological Regulation
https://www.readbyqxmd.com/read/28915625/long-term-cigarette-smoke-exposure-inhibits-histone-deacetylase-2-expression-and-enhances-the-nuclear-factor-%C3%AE%C2%BAb-activation-in-skeletal-muscle-of-mice
#7
Dongmei Huang, Zhiying Ma, Yili He, Ying Xiao, Honglin Luo, Qiuli Liang, Xiaoning Zhong, Jing Bai, Zhiyi He
Long-term cigarette smoke induces lung inflammatory injury and chronic obstructive pulmonary disease (COPD), associated with skeletal muscle inflammation. This study aimed at investigating how cigarette smoke promotes skeletal muscle inflammation and its molecular pathogenesis. Mice were exposed to air or cigarette smoke for 12 or 24 weeks, and C2C12 cells were stimulated with cigarette smoke extract (CSE). The mass and function, myotube formation, inflammatory cytokine production, histone deacetylase 2 (HDAC2) and nuclear factor-κB (NF-κB) p65 expression were detected in the gastrocnemius muscles of mice and C2C12 cells...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28912814/effect-of-antioxidant-water-on-the-bioactivities-of-cells
#8
Seong Gu Hwang, Ho-Sung Lee, Byung-Cheon Lee, GunWoong Bahng
It has been reported that water at the interface of a hydrophilic thin film forms an exclusion zone, which has a higher density than ordinary water. A similar phenomenon was observed for a hydrated hydrophilic ceramic powder, and water turns into a three-dimensional cell-like structure composed of high density water and low density water. This structured water appears to have a stimulative effect on plant growth. This report outlines our study of antioxidant properties of this structured water and its effect on cell bioactivities...
2017: International Journal of Cell Biology
https://www.readbyqxmd.com/read/28912572/follistatin-n-terminus-differentially-regulates-muscle-size-and-fat-in-vivo
#9
Hui Zheng, Chunping Qiao, Ruhang Tang, Jianbin Li, Karen Bulaklak, Zhenhua Huang, Chunxia Zhao, Yi Dai, Juan Li, Xiao Xiao
Delivery of follistatin (FST) represents a promising strategy for both muscular dystrophies and diabetes, as FST is a robust antagonist of myostatin and activin, which are critical regulators of skeletal muscle and adipose tissues. FST is a multi-domain protein, and deciphering the function of different domains will facilitate novel designs for FST-based therapy. Our study aims to investigate the role of the N-terminal domain (ND) of FST in regulating muscle and fat mass in vivo. Different FST constructs were created and packaged into the adeno-associated viral vector (AAV)...
September 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28905518/production-of-a-novel-medium-chain-length-poly-3-hydroxyalkanoate-using-unprocessed-biodiesel-waste-and-its-evaluation-as-a-tissue-engineering-scaffold
#10
Pooja Basnett, Barbara Lukasiewicz, Elena Marcello, Harpreet K Gura, Jonathan C Knowles, Ipsita Roy
This study demonstrated the utilization of unprocessed biodiesel waste as a carbon feedstock for Pseudomonas mendocinaCH50, for the production of PHAs. A PHA yield of 39.5% CDM was obtained using 5% (v/v) biodiesel waste substrate. Chemical analysis confirmed that the polymer produced was poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate) or P(3HHx-3HO-3HD-3HDD). P(3HHx-3HO-3HD-3HDD) was further characterized and evaluated for its use as a tissue engineering scaffold (TES)...
September 14, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28901427/microrna%C3%A2-106b-regulates-skeletal-muscle-insulin-sensitivity-and-glucose-homeostasis-by-targeting-mitofusion%C3%A2-2
#11
Ying Zhang, Wei He, Yuan-Fu Gao, Zhong-Min Fan, Chun-Lin Gao, Zheng-Kun Xia
MicroRNA‑106b (miR‑106b) is reported to be closely associated with skeletal muscle insulin resistance. The present study further investigated the role of miR‑106b in skeletal muscle insulin sensitivity and glucose homeostasis in vivo. Mice were randomly divided into 4 groups and infected with lentivirus expressing miR‑106b (miR‑106b mice), miR‑106b sponge (miR‑106b inhibition mice) or the corresponding empty vectors. Mitofusion‑2 (Mfn2) protein expression levels and glucose transporter (Glut)‑4 protein translocation were significantly reduced in the muscle of miR‑106b mice, whereas they were unaffected in miR‑106b inhibition mice...
September 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28899909/depletion-of-the-mrna-translation-initiation-inhibitor-programmed-cell-death-protein-4-pdcd4-impairs-l6-myotube-formation
#12
Naomi Maeda, Abdikarim Abdullahi, Brendan Beatty, Zameer Dhanani, Olasunkanmi A J Adegoke
The mechanistic (mammalian) target of rapamycin complex 1 (mTORC1) signaling is vital for optimal muscle mass and function. Although the significance of mTORC1 in stimulating muscle growth is unequivocal, evidence in support of its role during muscle regeneration is less clear. Here, we showed that the abundance (protein and mRNA) of the mTORC1/S6K1 substrate, programmed cell death protein 4 (PDCD4), is upregulated at the onset of differentiation of L6 and C2C12 cells. The increase in PDCD4 was not associated with any changes in S6K1 activation, but the abundance of beta transducing repeat-containing protein (β-TrCP), the ubiquitin ligase that targets PDCD4 for degradation, increased...
September 2017: Physiological Reports
https://www.readbyqxmd.com/read/28899780/hydrogen-peroxide-production-is-affected-by-oxygen-levels-in-mammalian-cell-culture
#13
Lucas A Maddalena, Shehab M Selim, Joao Fonseca, Holt Messner, Shannon McGowan, Jeffrey A Stuart
Although oxygen levels in the extracellular space of most mammalian tissues are just a few percent, under standard cell culture conditions they are not regulated and are often substantially higher. Some cellular sources of reactive oxygen species, like NADPH oxidase 4, are sensitive to oxygen levels in the range between 'normal' physiological (typically 1-5%) and standard cell culture (up to 18%). Hydrogen peroxide in particular participates in signal transduction pathways via protein redox modifications, so the potential increase in its production under standard cell culture conditions is important to understand...
September 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28895623/comprehensive-analytics-of-actovegin%C3%A2-and-its-effect-on-muscle-cells
#14
Franz-Xaver Reichl, Lesca Miriam Holdt, Daniel Teupser, Gregor Schütze, Alan J Metcalfe, Reinhard Hickel, Christof Högg, Wilhelm Bloch
The ingredients of Actovegin(®) were analyzed and its effects on the muscle cell proliferation were investigated. C2C12 myoblasts were cultured in medium. Actovegin(®) was added in five different concentrations (1, 5, 25, 125, and 250 µg) to the differentiation medium. The formations of proliferation factor Ki67 and myosin heavy chains were measured by immunofluorescence. The first primary antibody was anti-Ki67 and anti-Mf20. Cells were washed and treated with the second fluorochrome. Thirty-one Actovegin(®) ingredients were found to contain significantly higher concentrations and twenty-nine ingredients were found to contain significantly lower concentrations, compared to the mean ranges as described in the literature for the normal physiological concentrations in human adult serum/plasma...
September 11, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28894267/optogenetic-approach-for-targeted-activation-of-global-calcium-transients-in-differentiated-c2c12-myotubes
#15
Stéphane Sebille, Oualid Ayad, Charles-Albert Chapotte-Baldacci, Christian Cognard, Patrick Bois, Aurélien Chatelier
Excitation-contraction coupling in muscle cells is initiated by a restricted membrane depolarization delimited within the neuromuscular junction. This targeted depolarization triggers an action potential that propagates and induces a global cellular calcium response and a consequent contraction. To date, numerous studies have investigated this excitation-calcium response coupling by using different techniques to depolarize muscle cells. However, none of these techniques mimic the temporal and spatial resolution of membrane depolarization observed in the neuromuscular junction...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28889799/in-vitro-protective-effects-of-resveratrol-and-stilbene-alkanoic-derivatives-on-induced-oxidative-stress-on-c2c12-and-mcf7-cells
#16
L Leporini, L Giampietro, R Amororso, A Ammazzalorso, M Fantacuzzi, L Menghini, C Maccallini, C Ferrante, G Orlando, B De Filippis
Resveratrol (3,4’,5-trihydroxy-trans-stilbene) is a natural phytoalexin found in grapes and wine, which has been extensively studied for a wide range of biological effects. A large number of stilbene-containing derivatives have displayed antioxidant and antiproliferative activities on various cancer cell lines. In this study, a series of stilbene hybrids 1-9, previously reported as peroxisome proliferator-activated receptor (PPAR) agonists, were assessed at micromolar concentrations using MTT cell viability assay in C2C12 and MCF7 cell lines...
September 11, 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28888936/%C3%AE-arrestin-2-is-involved-in-irisin-induced-glucose-metabolism-in-type-2-diabetes-via-p38-mapk-signaling
#17
Yaling Pang, Haihui Zhu, Jianqin Xu, Lihua Yang, Lingjiao Liu, Jing Li
Type 2 diabetes mellitus (T2DM) is a common metabolic disease worldwide. It has been reported that irisin play regulatory role in glucose metabolism in T2DM. However, the underlying mechanism involved in that is not completely known. Herein, we determined the novel role of β-arrestin-2 in irisin-induced glucose utilization in diabetes. Effects of irisin and β-arrestin-2 on glucose utilization were investigated in a rat model of diabetes and in diabetic C2C12 cells in vitro. Results showed that irisin had positive role in glucose metabolism via regulating glucose tolerance as well as uptake in cardiac and skeletal muscle tissues, as evidenced by IPGTT, 2-deoxyglucose uptake and plasma membrane GLUT-4 assay...
September 6, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28887874/small-molecule-inhibition-of-murf1-attenuates-skeletal-muscle-atrophy-and-dysfunction-in-cardiac-cachexia
#18
Thomas Scott Bowen, Volker Adams, Sarah Werner, Tina Fischer, Paulien Vinke, Maria Noel Brogger, Norman Mangner, Axel Linke, Peter Sehr, Joe Lewis, Dittmar Labeit, Alexander Gasch, Siegfried Labeit
BACKGROUND: Muscle ring finger 1 (MuRF1) is a muscle-specific ubiquitin E3 ligase activated during clinical conditions associated with skeletal muscle wasting. Yet, there remains a paucity of therapeutic interventions that directly inhibit MuRF1 function, particularly in vivo. The current study, therefore, developed a novel compound targeting the central coiled coil domain of MuRF1 to inhibit muscle wasting in cardiac cachexia. METHODS: We identified small molecules that interfere with the MuRF1-titin interaction from a 130 000 compound screen based on Alpha Technology...
September 8, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/28886438/daidzein-down-regulates-ubiquitin-specific-protease-19-expression-through-estrogen-receptor-%C3%AE-and-increases-skeletal-muscle-mass-in-young-female-mice
#19
Masahiro Ogawa, Takehiro Kitano, Natsuha Kawata, Takashi Sugihira, Tomoya Kitakaze, Naoki Harada, Ryoichi Yamaji
Ubiquitin-specific protease 19 (USP19) is a key player in the negative regulation of muscle mass during muscle atrophy. Loss-of-function approaches demonstrate that 17β-estradiol (E2) increases USP19 expression through estrogen receptor (ER) α and consequently decreases soleus muscle mass in young female mice under physiological conditions. Daidzein is one of the main isoflavones in soy, and activates ERβ-dependent transcription. Here, we investigated the effects of daidzein on E2-increased USP19 expression and E2-decreased soleus muscle mass in young female mice...
August 12, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28886070/microrna-mir-301a-is-a-novel-cardiac-regulator-of-cofilin-2
#20
Ashraf Yusuf Rangrez, Phillip Hoppe, Christian Kuhn, Elisa Zille, Johanne Frank, Norbert Frey, Derk Frank
Calsarcin-1 deficient mice develop dilated cardiomyopathy (DCM) phenotype in pure C57BL/6 genetic background (Cs1-ko) despite severe contractile dysfunction and robust activation of fetal gene program. Here we performed a microRNA microarray to identify the molecular causes of this cardiac phenotype that revealed the dysregulation of several microRNAs including miR-301a, which was highly downregulated in Cs1-ko mice compared to the wild-type littermates. Cofilin-2 (Cfl2) was identified as one of the potential targets of miR-301a using prediction databases, which we validated by luciferase assay and mutation of predicted binding sites...
2017: PloS One
keyword
keyword
22712
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"