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https://www.readbyqxmd.com/read/28644396/runx1-plays-an-important-role-in-mediating-bmp9-induced-osteogenic-differentiation-of-mesenchymal-stem-cells-line-c3h10t1-2-murine-multi-lineage-cells-lines-c2c12-and-mefs
#1
Caixia Ji, Xiaohua Liu, Li Xu, Tingting Yu, Chaoqun Dong, Jinyong Luo
As one of the least studied bone morphogenetic proteins (BMPs), BMP9 is highly capable of promoting osteogenic differentiation. However, the underlying mechanism involved remains largely unknown. Recent studies have demonstrated that RUNX1 (runt-related transcription factor 1) is essential in osteoblast/chondrocyte maturation. In this study, we investigated the function of RUNX1 in BMP9-induced osteogenic of murine mesenchymal stem cell line (C3H10T1/2) and murine multi-lineage cell lines (C2C12 and MEFs). Our data showed that BMP9 promoted the endogenous expression of RUNX1 in C3H10T1/2, C2C12 and MEFs...
June 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28639790/programmable-assembly-of-peptide-amphiphile-via-noncovalent-to-covalent-bond-conversion
#2
Kohei Sato, Wei Ji, Liam C Palmer, Benjamin Weber, Matthias Barz, Samuel I Stupp
Controlling the number of monomers in a supramolecular polymer has been a great challenge in programmable self-assembly of organic molecules. One approach has been to make use of frustrated growth of the supramolecular assembly by tuning the balance of attractive and repulsive intermolecular forces. We report here on the use of covalent bond formation among monomers, compensating for intermolecular electrostatic repulsion, as a mechanism to control the length of a supramolecular nanofiber formed by self-assembly of peptide amphiphiles...
June 22, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28638997/follistatin-treatment-suppresses-serca1b-levels-independently-of-other-players-of-calcium-homeostasis-in-c2c12-myotubes
#3
János Fodor, Adrienn Gomba-Tóth, Tamás Oláh, János Almássy, Ernő Zádor, László Csernoch
Follistatin (FS) is a high affinity activin-binding protein, neutralizing the effects of the Transforming Growth Factor-beta (TGF-β) superfamily members, as myostatin (MSTN). Since MSTN emerged as a negative regulator, FS has been considered as a stimulator of skeletal muscle growth and differentiation. Here, we studied the effect of FS administration on the Ca(2+)-homeostasis of differentiating C2C12 skeletal muscle cells. FS-treatment increased the fusion index, the size of terminally differentiated myotubes, and transiently elevated the expression of the calcium-dependent protein phosphatase, calcineurin, at the beginning of differentiation...
June 21, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28634932/preparation-of-decellularized-biological-scaffolds-for-3d-cell-culture
#4
Bryan N Brown, Michael J Buckenmeyer, Travis A Prest
The biggest challenge of designing and implementing an in vitro study is developing a microenvironment that most closely represents the interactions observed in vivo. Decellularization of tissues and organs has been shown to be an effective method for the removal of potentially immunogenic constituents while preserving essential growth factors and extracellular matrix (ECM ) proteins necessary for proper cell function. Enzymatic digestion of decellularized tissues allows these tissue-specific components to be reconstituted into bioactive hydrogels through a physical crosslinking of collagen...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28634643/acute-high-caffeine-exposure-increases-autophagic-flux-and-reduces-protein-synthesis-in-c2c12-skeletal-myotubes
#5
M A Hughes, R M Downs, G W Webb, C L Crocker, S T Kinsey, Bradley L Baumgarner
Caffeine is a highly catabolic dietary stimulant. High caffeine concentrations (1-10 mM) have previously been shown to inhibit protein synthesis and increase protein degradation in various mammalian cell lines. The purpose of this study was to examine the effect of short-term caffeine exposure on cell signaling pathways that regulate protein metabolism in mammalian skeletal muscle cells. Fully differentiated C2C12 skeletal myotubes either received vehicle (DMSO) or 5 mM caffeine for 6 h. Our analysis revealed that caffeine promoted a 40% increase in autolysosome formation and a 25% increase in autophagic flux...
June 20, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28627736/enzyme-free-cell-detachment-mediated-by-resonance-vibration-with-temperature-modulation
#6
Yuta Kurashina, Makoto Hirano, Chikahiro Imashiro, Kiichiro Totani, Jun Komotori, Kenjiro Takemura
Cell detachment is an essential process in adherent cell culture. However, trypsinization, which is the most popular detachment technique used in culture, damages cellular membranes. Reducing cellular membrane damage during detachment should improve the quality of cell culture. In this article, we propose an enzyme-free cell detachment method based on resonance vibration with temperature modulation. We developed a culture device that can excite a resonance vibration and control temperature. We then evaluated the cell detachment ratio and the growth response, observed the morphology and analyzed the cellular protein of the collected cells-mouse myoblast cell line (C2C12)...
June 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28626780/igg-specific-cell-based-assay-detects-potentially-pathogenic-musk-abs-in-seronegative-mg
#7
Saif Huda, Patrick Waters, Mark Woodhall, Maria Isabel Leite, Leslie Jacobson, Anna De Rosa, Michelangelo Maestri, Roberta Ricciardi, Jeannine M Heckmann, Angelina Maniaol, Amelia Evoli, Judy Cossins, David Hilton-Jones, Angela Vincent
OBJECTIVE: To increase the detection of MuSK-Abs using a CBA and test their pathogenicity. METHODS: Sera from 69 MuSK-RIA-positive patients with myasthenia gravis (MG) (Definite MuSK-MG), 169 patients negative for MuSK-RIA and AChR-RIA (seronegative MG, SNMG), 35 healthy individuals (healthy controls, HCs), and 16 NMDA receptor-Ab-positive (NMDAR-Ab) disease controls were tested for binding to MuSK on a CBA using different secondary antibodies. RESULTS: Initially, in addition to 18% of SNMG sera, 11% of HC and 19% of NMDAR-Ab sera showed positive binding to MuSK-transfected cells; this low specificity was due to anti-IgG(H+L) detection of IgM bound nonspecifically to MuSK...
July 2017: Neurology® Neuroimmunology & Neuroinflammation
https://www.readbyqxmd.com/read/28623958/knockdown-of-subunit-3-of-the-cop9-signalosome-inhibits-c2c12-myoblast-differentiation-via-nf-kappab-signaling-pathway
#8
Mariam A Ba, Jeffrey Surina, Cherie A Singer, Maria L Valencik
BACKGROUND: The COP9 signalosome (CSN) is a conserved protein complex composed of 8 subunits designated CSN1-CSN8. CSN3 represents the third subunit of the CSN and maintains the integrity of the complex. CSN3 binds to the striated muscle-specific β1D integrin tail, and its subcellular localization is altered in differentiated skeletal muscle cells. However, the role of CSN3 in skeletal muscle differentiation is unknown. The main goal of this study was to identify whether CSN3 participates in myoblast differentiation and the signalling mechanisms involved using C2C12 cells as a skeletal muscle cell model...
June 17, 2017: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28622706/electrical-stimulation-of-microengineered-skeletal-muscle-tissue-effect-of-stimulus-parameters-on-myotube-contractility-and-maturation
#9
Ramin Banan Sadeghian, Majid Ebrahimi, Sahar Salehi
Skeletal muscle tissues engineered in vitro are aneural, short in number of fibers required to function properly, and degenerate rapidly. Electrical stimulation has been widely used to compensate for such lack of neural activity, yet the relationship between the stimulation parameters and the tissue response is subject of debate. Here we study the effect of overnight electrical stimulation (training) on the contractility and maturity of aligned C2C12 myotubes developed on micropatterned gelatin methacryloyl (GelMA) substrates...
June 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28609469/muscle-relevant-genes-marked-by-stable-h3k4me2-3-profiles-and-enriched-myod-binding-during-myogenic-differentiation
#10
Huanhuan Cui, Vikas Bansal, Marcel Grunert, Barbora Malecova, Alessandra Dall'Agnese, Lucia Latella, Sole Gatto, Tammy Ryan, Kerstin Schulz, Wei Chen, Cornelia Dorn, Pier Lorenzo Puri, Silke R Sperling
Post-translational modifications of histones play a key role in the regulation of gene expression during development and differentiation. Numerous studies have shown the dynamics of combinatorial regulation by transcription factors and histone modifications, in the sense that different combinations lead to distinct expression outcomes. Here, we investigated gene regulation by stable enrichment patterns of histone marks H3K4me2 and H3K4me3 in combination with the chromatin binding of the muscle tissue-specific transcription factor MyoD during myogenic differentiation of C2C12 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28608935/cdk9-regulates-apoptosis-of-myoblast-cells-by-modulation-of-microrna-1-expression
#11
Vahideh Tarhriz, Kay-Dietrich Wagner, Zahra Masoumi, Ommoleila Molavi, Mohammad Saeid Hejazi, Hossein Ghanbarian
Cdk9 is the catalytic core of the positive transcription elongation factor b (P-TEFb) and regulates transcriptional elongation factors by phosphorylation of RNA pol II. Apart from its role on myogenic gene expression, Cdk9 regulation of muscle-specific microRNAs in the early stage of cardiomyogenesis is poorly understood. Here we demonstrate that Cdk9 not only regulates myogenic transcription factors, but also controls muscle-specific microRNAs. During cardiac differentiation of mouse embryonic stem cells, high Cdk9 expression preceded up-regulation of miR-1...
June 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28608457/potential-therapeutic-interventions-for-chronic-kidney-disease-associated-sarcopenia-via-indoxyl-sulfate-induced-mitochondrial-dysfunction
#12
Yuki Enoki, Hiroshi Watanabe, Riho Arake, Rui Fujimura, Kana Ishiodori, Tadashi Imafuku, Kento Nishida, Ryusei Sugimoto, Saori Nagao, Shigeyuki Miyamura, Yu Ishima, Motoko Tanaka, Kazutaka Matsushita, Hirotaka Komaba, Masafumi Fukagawa, Masaki Otagiri, Toru Maruyama
BACKGROUND: Chronic kidney disease (CKD) patients experience skeletal muscle wasting and decreased exercise endurance. Our previous study demonstrated that indoxyl sulfate (IS), a uremic toxin, accelerates skeletal muscle atrophy. The purpose of this study was to examine the issue of whether IS causes mitochondria dysfunction and IS-targeted intervention using AST-120, which inhibits IS accumulation, or mitochondria-targeted intervention using L-carnitine or teneligliptin, a dipeptidyl peptidase-4 inhibitor which retains mitochondria function and alleviates skeletal muscle atrophy and muscle endurance in chronic kidney disease mice...
June 12, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/28607928/extremely-low-frequency-electromagnetic-fields-affect-myogenic-processes-in-c2c12-myoblasts-role-of-gap-junction-mediated-intercellular-communication
#13
Caterina Morabito, Nathalie Steimberg, Francesca Rovetta, Jennifer Boniotti, Simone Guarnieri, Giovanna Mazzoleni, Maria A Mariggiò
Extremely low-frequency electromagnetic fields (ELF-EMFs) can interact with biological systems. Although they are successfully used as therapeutic agents in physiatrics and rehabilitative practice, they might represent environmental pollutants and pose a risk to human health. Due to the lack of evidence of their mechanism of action, the effects of ELF-EMFs on differentiation processes in skeletal muscle were investigated. C2C12 myoblasts were exposed to ELF-EMFs generated by a solenoid. The effects of ELF-EMFs on cell viability and on growth and differentiation rates were studied using colorimetric and vital dye assays, cytomorphology, and molecular analysis of MyoD and myogenin expression, respectively...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28607575/spatholobus-suberectus-exhibits-antidiabetic-activity-in-vitro-and-in-vivo-through-activation-of-akt-ampk-pathway
#14
Peijun Zhao, Md Badrul Alam, Seok-Hyun Lee, Young-Jun Kim, Seul Lee, Hongyan An, Hee-Jeong Choi, Hyeong-U Son, Chul-Hong Park, Hyo-Hyun Kim, Sang-Han Lee
Glucose deposition in peripheral tissue is an important parameter for the treatment of type 2 diabetes mellitus. The aim of this study was to investigate the effects of Spatholobus suberectus (Ss) on glucose disposal in skeletal muscle cells and additionally explore its in vivo antidiabetic potential. Treatment of ethanolic extract of S. suberectus (EeSs) significantly enhanced the glucose uptake, mediated through the enhanced expression of GLUT4 in skeletal muscle via the stimulation of AKT and AMPK pathways in C2C12 cells...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28605780/uremia-induces-adipose-tissue-inflammation-and-muscle-mitochondrial-dysfunction
#15
Maria P Martinez Cantarin, Diana Whitaker-Menezes, Zhao Lin, Bonita Falkner
Background.: End-stage renal disease (ESRD) is associated with inflammation and increased reactive oxygen species (ROS). Inflammation and oxidative stress are associated with several complications of ESRD. The aim of this study was to determine histological characteristics of adipose tissue and muscle mitochondrial function in uremia and its relationship with inflammation. Methods.: ESRD patients ( n  = 18) and controls ( n  = 6) were enrolled for studies of adipose and muscle tissue by immunohistochemistry and western blot...
June 1, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28601071/two-variants-of-recombinant-human-bone-morphogenetic-protein-2-rhbmp-2-with-additional-protein-domains-synthesis-in-an-escherichia-coli-heterologous-expression-system
#16
A S Karyagina, I S Boksha, T M Grunina, A V Demidenko, M S Poponova, O V Sergienko, A M Lyashchuk, Z M Galushkina, L A Soboleva, E O Osidak, M S Bartov, A V Gromov, V G Lunin
Two variants of recombinant human bone morphogenetic protein-2 (rhBMP-2) with additional N-terminal protein domains were obtained by expression in E. coli. The N-terminal domains were s-tag (15-a.a. oligopeptide from bovine pancreatic ribonuclease A) and lz (leucine zipper dimerization domain from yeast transcription factor GCN4). The s-tag-BMP-2 and lz-BMP-2 were purified by a procedure that excluded a long refolding stage. The resulting dimeric proteins displayed higher solubility compared to rhBMP-2 without additional protein domains...
May 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28600493/adiponectin-modulates-oxidative-stress-induced-mitophagy-and-protects-c2c12-myoblasts-against-apoptosis
#17
Yinghui Ren, Yan Li, Jun Yan, Mingkun Ma, Dongmei Zhou, Zhenyi Xue, Zimu Zhang, Hongkun Liu, Huipeng Yang, Long Jia, Lijuan Zhang, Qi Zhang, Shuqin Mu, Rongxin Zhang, Yurong Da
Adiponectin (APN), also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone that is predominantly produced by adipose tissue. Many pharmacological studies have demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory properties. Although several studies have demonstrated the antioxidative activity of this protein, the regulatory mechanisms have not yet been defined in skeletal muscles. The aim of the present study was to examine the cytoprotective effects of APN against damage induced by oxidative stress in mouse-derived C2C12 myoblasts...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28599297/long-term-cigarette-smoke-exposure-inhibits-histone-deacetylase-2-expression-and-enhances-the-nuclear-factor-%C3%AE%C2%BAb-activation-in-skeletal-muscle-of-mice
#18
Dongmei Huang, Zhiying Ma, Yili He, Ying Xiao, Honglin Luo, Qiuli Liang, Xiaoning Zhong, Jing Bai, Zhiyi He
Long-term cigarette smoke induces lung inflammatory injury and chronic obstructive pulmonary disease (COPD), associated with skeletal muscle inflammation. This study aimed at investigating how cigarette smoke promotes skeletal muscle inflammation and its molecular pathogenesis. Mice were exposed to air or cigarette smoke for 12 or 24 weeks, and C2C12 cells were stimulated with cigarette smoke extract (CSE). The mass and function, myotube formation, inflammatory cytokine production, histone deacetylase 2 (HDAC2) and nuclear factor-κB (NF-κB) p65 expression were detected in the gastrocnemius muscles of mice and C2C12 cells...
May 23, 2017: Oncotarget
https://www.readbyqxmd.com/read/28589421/tat-myod-fused-proteins-together-with-c2c12-conditioned-medium-are-able-to-induce-equine-adult-mesenchimal-stem-cells-towards-the-myogenic-fate
#19
Marco Patruno, Chiara Gomiero, Roberta Sacchetto, Ohad Topel, Alessandro Negro, Tiziana Martinello
The Tat protein is able to translocate through the plasma membrane and when it is fused with other peptides may acts as a protein transduction system. This ability appears particularly interesting to induce tissue-specific differentiation when the Tat protein is associated to transcription factors. In the present work, the potential of the complex Tat-MyoD in inducing equine peripheral blood mesenchymal stem cells (PB-MSCs) towards the myogenic fate, was evaluated. Results showed that the internalization process of Tat-MyoD happens only in serum free conditions and that the nuclear localization of the fused complex is observed after 15 hours of incubation...
June 6, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28588499/intake-of-a-ketone-ester-drink-during-recovery-from-exercise-promotes-mtorc1-signaling-but-not-glycogen-resynthesis-in-human-muscle
#20
Tijs Vandoorne, Stefan De Smet, Monique Ramaekers, Ruud Van Thienen, Katrien De Bock, Kieran Clarke, Peter Hespel
Purpose: Ketone bodies are energy substrates produced by the liver during prolonged fasting or low-carbohydrate diet. The ingestion of a ketone ester (KE) rapidly increases blood ketone levels independent of nutritional status. KE has recently been shown to improve exercise performance, but whether it can also promote post-exercise muscle protein or glycogen synthesis is unknown. Methods: Eight healthy trained males participated in a randomized double-blind placebo-controlled crossover study. In each session, subjects undertook a bout of intense one-leg glycogen-depleting exercise followed by a 5-h recovery period during which they ingested a protein/carbohydrate mixture...
2017: Frontiers in Physiology
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