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https://www.readbyqxmd.com/read/28812353/dopamine-incorporated-dual-bioactive-electroactive-shape-memory-polyurethane-elastomers-with-physiological-shape-recovery-temperature-high-stretchability-and-enhanced-c2c12-myogenic-differentiation
#1
Xin Zhao, Ruonan Dong, Baolin Guo, Peter X Ma
Soft tissue engineering needs elastic biomaterials not only mimicking the elasticity of soft tissue but also possessing multiple bioactivity to promote cell adhesion, proliferation and differentiation, which still remains ongoing challenges. Herein, we synthesized a series of dopamine-incorporated dual bioactive electroactive shape memory polyurethane elastomers by combining the properties of elastomeric poly(citric acid-co-polycaprolactone) (CA-PCL) polyurethane elastomer, bioactive dopamine (DA) and electroactive aniline hexamer (AH)...
August 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28811296/a-novel-d2o-tracer-method-to-quantify-rna-turnover-as-a-biomarker-of-de-novo-ribosomal-biogenesis-in-vitro-in-animal-models-and-in-human-skeletal-muscle
#2
Matthew S Brook, Daniel J Wilkinson, W Kyle Mitchell, Jonathan L Lund, Bethan E Phillips, Nathaniel J Sweczyk, Heikki Kainulainen, Sanna Lensu, Lauren G Koch, Steve L Britton, Paul L Greenhaff, Kenneth Smith, Philip J Atherton
Current methods to quantify RNA dynamics are beset with limitations. Here, we developed a novel stable isotope (D2O)-methodology to quantify RNA synthesis (i.e. ribosomal biogenesis) in cells, animal models, and humans. Firstly, proliferating C2C12 cells were incubated in D2O-enriched media and myotubes (±) 50ng.ml(-1) IGF-1. Secondly, rat quadriceps [untrained n=9; 7-wks interval-"like" training n=13] were collected after ~3-wks D2O (70-Atom%) administration, with body-water enrichment (BWE) monitored via blood sampling...
August 15, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28811203/bpn-a-marine-derived-ptp1b-inhibitor-activates-insulin-signaling-and-improves-insulin-resistance-in-c2c12-myotubes
#3
Qi Xu, Jiao Luo, Ning Wu, Renshuai Zhang, Dayong Shi
Insulin resistance is a key feature of type 2 diabetes mellitus (T2DM) and is characterized by defects in insulin signaling. Protein tyrosine phosphatase 1B (PTP1B) is a major negative regulator of insulin signaling cascade and has attracted intensive investigation in recent T2DM therapy study. BPN, a marine-derived bromophenol compound, was isolated from the red alga Rhodomela confervoides. This study investigated the effects of BPN on the insulin signaling pathway in insulin-resistant C2C12 myotubes by inhibiting PTP1B...
August 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28803986/ubiquitin-c-terminal-hydrolase-l1-regulates-myoblast-proliferation-and-differentiation
#4
Hongbo Gao, Sigurd Hartnett, Yifan Li
Skeletal muscles are dynamic tissues that possess regenerative abilities, which require multiple processes and regulatory factors. Ubiquitin C-Terminal Hydrolase L1 (UCHL1), which is primarily expressed in neuronal tissues, was upregulated in skeletal muscles in disease conditions but its functional role in skeletal muscles is unknown. Using mouse myoblast cells C2C12 as an in vitro model, this study reported that UCHL1 elicits different regulation in myoblast cell proliferation and differentiation. We first observed that UCHL1 protein level was continuously declined during cell differentiation...
August 10, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28792773/easy-to-apply-polyoxazoline-based-coating-for-precise-and-long-term-control-of-neural-patterns
#5
Serge Weydert, Stefan Zürcher, Stefanie Tanner, Ning Zhang, Rebecca Ritter, Thomas Peter, Mathias J Aebersold, Greta Thompson-Steckel, Csaba Forró, Markus Rottmar, Flurin Stauffer, Irene A Valassina, Giulia Morgese, Edmondo M Benetti, Samuele Tosatti, János Vörös
Arranging cultured cells in patterns via surface modification is a tool used by biologists to answer questions in a specific and controlled manner. In the past decade, bottom-up neuroscience emerged as a new application, which aims to get a better understanding of the brain via reverse engineering and analyzing elementary circuitry in vitro. Building well-defined neural networks is the ultimate goal. Antifouling coatings are often used to control neurite outgrowth. Because erroneous connectivity alters the entire topology and functionality of minicircuits, the requirements are demanding...
August 9, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28792734/biocompatible-elastic-conductive-films-significantly-enhanced-myogenic-differentiation-of-myoblast-for-skeletal-muscle-regeneration
#6
Ruonan Dong, Xin Zhao, Baolin Guo, Peter X Ma
The key factor in skeletal muscle tissue engineering is regeneration of the functional skeletal muscles. Materials that could promote the myoblast proliferation and myogenic differentiation are promising candidates in skeletal muscle tissue engineering. Herein, we developed an elastic conductive poly(ethylene glycol)-co-poly(glycerol sebacate) (PEGS) grafted aniline pentamer (AP) copolymer that could promote the formation of myotubes by differentiating the C2C12 myoblast cells. The results of hydration behavior and water contact angle suggested that by adjusting the poly(ethylene glycol) (PEG) and AP content, this film showed a proper surface hydrophilicity for cell attachment...
August 9, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28791357/synergistic-effects-of-fibroblast-growth-factor-2-and-bone-morphogenetic-protein-2-on-bone-induction
#7
Rongying Song, Dingding Wang, Rong Zeng, Ju Wang
The present study investigated the synergistic effect of co‑administering fibroblast growth factor‑2 (FGF‑2) and bone morphogenetic protein‑2 (BMP‑2) on osteoblastic differentiation in C2C12 cells and in rats. C2C12 murine myoblast cells represent a well‑accepted in vitro model system to study the ability of BMP‑2 to alter cell lineage from the myogenic to the osteogenic phenotype. The osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) and Alizarin red S staining...
August 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28790924/effects-of-ionizing-irradiation-on-mouse-diaphragmatic-skeletal-muscle
#8
Tingyang Zhou, Lanchun Lu, Shiyong Wu, Li Zuo
Undesirable exposure of diaphragm to radiation during thoracic radiation therapy has not been fully considered over the past decades. Our study aims to examine the potential biological effects on diaphragm induced by radiation. One-time ionizing irradiation of 10 Gy was applied either to the diaphragmatic region of mice or to the cultured C2C12 myocytes. Each sample was then assayed for muscle function, oxidative stress, or cell viability on days 1, 3, 5, and 7 after irradiation. Our mouse model shows that radiation significantly reduced muscle function on the 5th and 7th days and increased reactive oxygen species (ROS) formation in the diaphragm tissue from days 3 to 7...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28790171/the-ror1-receptor-tyrosine-kinase-plays-a-critical-role-in-regulating-satellite-cell-proliferation-during-regeneration-of-injured-muscle
#9
Koki Kamizaki, Ryosuke Doi, Makoto Hayashi, Takeshi Saji, Motoi Kanagawa, Tatsushi Toda, So-Ichiro Fukada, Hsin-Yi Henry Ho, Michael Eldon Greenberg, Mitsuharu Endo, Yasuhiro Minami
The Ror family receptor tyrosine kinases, Ror1 and Ror2 play important roles in regulating developmental morphogenesis and tissue- and organogenesis, but their roles in tissue regeneration in adult animals remains largely unknown. In this study, we examined the expression and function of Ror1 and Ror2 during skeletal muscle regeneration. Using an in vivo skeletal muscle injury model, we show that expression of Ror1 and Ror2 in skeletal muscles is induced transiently by the inflammatory cytokines, TNF-α and IL-1β, after injury, and that inhibition of TNF-α and IL-1β by neutralizing antibodies suppresses expression of Ror1 and Ror2 in injured muscles...
August 8, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28782600/mir-18a-induces-myotubes-atrophy-by-down-regulating-igfi
#10
Chuncheng Liu, Meng Wang, Min Chen, Kuo Zhang, Lijie Gu, Qiuyan Li, Zhengquan Yu, Ning Li, Qingyong Meng
Muscle atrophy occurs when there is a net loss of muscle mass, leading to a change in the balance between protein synthesis and protein degradation. Igf1 is important for protein synthesis in muscle cells and can induce local skeletal muscle hypertrophy and attenuate age-related skeletal muscle atrophy via the PI3K/Akt pathway in mice, consequently restoring and improving muscle mass and strength. In this study, we show that miR-18a expression is down-regulated during C2C12 myoblast differentiation and mouse tibialis anterior muscle postnatal development...
August 4, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28782556/an-sirna-based-screen-in-c2c12-myoblasts-identifies-novel-genes-involved-in-myogenic-differentiation
#11
Rayan Alwan, Ange-Line Bruel, Anne Da Silva, Véronique Blanquet, Khaled Bouhouche
Myogenesis is a highly regulated multi-step process involving myoblast proliferation and differentiation. Although studies over the last decades have identified several factors governing these distinct major phases, many of them are not yet known. In order to identify novel genes, we took advantage of the C2C12 myoblastic line to establish a functional siRNA screen combined with quantitative-imaging analysis of a large amount of differentiated myoblasts. We knocked down 100 preselected mouse genes without a previously characterized role in muscle...
August 3, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28775321/mir-23-trxr1-as-a-novel-molecular-axis-in-skeletal-muscle-differentiation
#12
Neri Mercatelli, Simona Fittipaldi, Elisa De Paola, Ivan Dimauro, Maria Paola Paronetto, Malcolm J Jackson, Daniela Caporossi
Thioredoxin reductase 1 (TrxR1) is a selenocysteine-containing protein involved in cellular redox homeostasis which is downregulated in skeletal muscle differentiation. Here we show that TrxR1 decrease occurring during myogenesis is functionally involved in the coordination of this cellular process. Indeed, TrxR1 depletion reduces myoblasts growth by inducing an early myogenesis -related gene expression pattern which includes myogenin and Myf5 up-regulation and Cyclin D1 decrease. On the contrary, the overexpression of TrxR1 during differentiation delays myogenic process, by negatively affecting the expression of Myogenin and MyHC...
August 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28768641/acute-myotube-protein-synthesis-regulation-by-il-6-related-cytokines
#13
Song Gao, J Larry Durstine, Ho-Jin Koh, Wayne E Carver, Norma Frizzell, James A Carson
IL-6 and LIF, members of the IL-6 family of cytokines, play recognized paradoxical roles in skeletal muscle mass regulation, being associated with both growth and atrophy. Overload or muscle contractions can induce a transient increase in muscle IL-6 and LIF expression, which has a regulatory role in muscle hypertrophy. However, the cellular mechanisms involved in this regulation have not been completely identified. The induction of mTORC1-dependent myofiber protein synthesis is an established regulator of muscle hypertrophy, but the involvement of the IL-6 family of cytokines in this process is poorly understood...
August 2, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28765595/integration-of-mirna-and-mrna-expression-profiles-reveals-microrna-regulated-networks-during-muscle-wasting-in-cardiac-cachexia
#14
Leonardo N Moraes, Geysson J Fernandez, Ivan J Vechetti-Júnior, Paula P Freire, Rodrigo W A Souza, Rolando A R Villacis, Silvia R Rogatto, Patricia P Reis, Maeli Dal-Pai-Silva, Robson F Carvalho
Cardiac cachexia (CC) is a common complication of heart failure (HF) associated with muscle wasting and poor patient prognosis. Although different mechanisms have been proposed to explain muscle wasting during CC, its pathogenesis is still not understood. Here, we described an integrative analysis between miRNA and mRNA expression profiles of muscle wasting during CC. Global gene expression profiling identified 1,281 genes and 19 miRNAs differentially expressed in muscle wasting during CC. Several of these deregulated genes are known or putative targets of the altered miRNAs, including miR-29a-3p, miR-29b-3p, miR-210-5p, miR-214, and miR-489...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28765271/sirt6-regulates-metabolic-homeostasis-in-skeletal-muscle-through-activation-of-ampk
#15
Xiaona Cui, Lu Yao, Xiaoying Yang, Yong Gao, Fude Fang, Jun Zhang, Qinghua Wang, Yongsheng Chang
Due to the mass and functions in metabolism, skeletal muscle is one of major organs regulating whole-body metabolic homeostasis. SIRT6, a histone deacetylase, has been shown to regulate metabolism in liver and brain, however, its specific role in skeletal muscle is undetermined. In the present study we explored physiological function of SIRT6 in muscle. We generated a muscle-specific SIRT6 knockout mouse model. The mice with SIRT6 deficiency in muscle displayed impaired glucose homeostasis and insulin sensitivity, attenuated whole-body energy expenditure and weakened exercise performance...
August 1, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28762415/biocompatible-3d-printed-polymers-via-fused-deposition-modelling-direct-c2c12-cellular-phenotype-in-vitro
#16
Rowan P Rimington, Andrew J Capel, Steven D R Christie, Mark P Lewis
The capability to 3D print bespoke biologically receptive parts within short time periods has driven the growing prevalence of additive manufacture (AM) technology within biological settings, however limited research concerning cellular interaction with 3D printed polymers has been undertaken. In this work, we used skeletal muscle C2C12 cell line in order to ascertain critical evidence of cellular behaviour in response to multiple bio-receptive candidate polymers; polylactic acid (PLA), acrylonitrile butadiene styrene (ABS), polyethylene terephthalate (PET) and polycarbonate (PC) 3D printed via fused deposition modelling (FDM)...
August 1, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28757463/permethrin-alters-glucose-metabolism-in-conjunction-with-high-fat-diet-by-potentiating-insulin-resistance-and-decreases-voluntary-activities-in-female-c57bl-6j-mice
#17
Xiao Xiao, Yoo Kim, Daeyoung Kim, Kyong Sup Yoon, John M Clark, Yeonhwa Park
Permethrin, a type 1 pyrethroid insecticide, was previously reported to promote adipogenesis in 3T3-L1 adipocytes and insulin resistance in C2C12 muscle cells; however, the effects of permethrin exposure on glucose and lipid metabolisms in vivo remain unknown. The purpose of this study was to investigate the effects of permethrin exposure on glucose and lipid homeostasis as well as voluntary movement in female mice in response to dietary fat. We tested three doses of permethrin (50, 500, & 5000 μg/kg body weight/day) in low fat diet-fed (4% w/w of diet) and high fat diet-fed (20% w/w of diet) female C57BL/6 J mice for twelve weeks...
July 28, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28757237/ultrasound-induced-strain-cytoskeleton-rearrangement-an-experimental-and-simulation-study
#18
Mohamadmahdi Samandari, Karen Abrinia, Manijhe Mokhtari-Dizaji, Ali Tamayol
Cytoskeleton and specially actin filaments are responsible for mechanical modulation of cellular behavior. These structures could be fluidized in response to transient mechanical cues. Ultrasound devices have been widely used in medicine which their generated ultrasonic waves could disrupt/fluidize actin filaments in cytoskeleton and thus could affect cellular organization. Present research aims at revealing the mechanism of fluidization caused by ultrasound induced strains. First, a numerical simulation was performed to reveal the effect of oscillating ultrasonic pressure on induced deformation in the cell with respect to different cell geometries and exposure conditions...
June 20, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/28754837/p53-independent-dux4-pathology
#19
Darko Bosnakovski, Micah D Gearhart, Erik A Toso, Olivia O Recht, Anja Cucak, Abhinav K Jain, Michelle C Barton, Michael Kyba
FSHD is a genetically dominant myopathy caused by mutations that disrupt repression of the normally silent DUX4 gene, which encodes a transcription factor that has been shown to interfere with myogenesis when misexpressed at very low levels in myoblasts, and to cause cell death when overexpressed at high levels. A previous report using adeno-associated virus to deliver high levels of DUX4 to mouse skeletal muscle demonstrated severe pathology that was suppressed on a p53 knockout background, implying that DUX4 acted through the p53 pathway...
July 28, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28754631/the-nuclear-protein-coding-gene-ankrd23-negatively-regulates-myoblast-differentiation
#20
Xiaojing Wang, Rui Zeng, Haiyang Xu, Zaiyan Xu, Bo Zuo
Muscle fiber formation is a complex process and subject to fine regulation of a variety of protein-coding genes and non-coding RNA. In this study, we identified a nuclear protein-coding gene ANKRD23 which was highly expressed in muscle. Quantitative real-time PCR, western blotting and immunofluorescence were used to detect the expression change of myoblast differentiation marker genes after knockdown and overexpression of ANKRD23. The results showed that the expression of myoblast differentiation marker genes were increased by interference and reduced by ANKRD23 overexpression, indicating that ANKRD23 played a negative role in the myoblast differentiation...
July 25, 2017: Gene
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