Cecilia Contardi, Davide Rubes, Massimo Serra, Rossella Dorati, Marco Dattilo, Liliia Mavliutova, Maddalena Patrini, Raffaella Guglielmann, Börje Sellergren, Ersilia De Lorenzi
In this work, an innovative and accurate affinity capillary electrophoresis (ACE) method was set up to monitor the complexation of aqueous MIP nanogels (NGs) with model cancer-related antigens. Using α2,6'- and α2,3'-sialyllactose as oversimplified cancer biomarker-mimicking templates, NGs were synthesized and characterized in terms of size, polydispersity, and overall charge. A stability study was also carried out in order to select the best storage conditions and to ensure product quality. After optimization of capillary electrophoresis conditions, injection of MIP NGs resulted in a single, sharp, and efficient peak...
January 29, 2024: Analytical Chemistry