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https://www.readbyqxmd.com/read/28222368/direct-and-label-free-influenza-virus-detection-based-on-multisite-binding-to-sialic-acid-receptors
#1
Yukichi Horiguchi, Tatsuro Goda, Akira Matsumoto, Hiroaki Takeuchi, Shoji Yamaoka, Yuji Miyahara
A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Acα(2-6)Galβ(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces...
June 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28192976/quantification-of-influenza-neuraminidase-activity-by-ultra-high-performance-liquid-chromatography-and-isotope-dilution-mass-spectrometry
#2
Maria I Solano, Adrian R Woolfitt, Tracie L Williams, Carrie L Pierce, Larisa V Gubareva, Vasiliy Mishin, John R Barr
Mounting evidence suggests that neuraminidase's functionality extends beyond its classical role in influenza virus infection and that antineuraminidase antibodies offer protective immunity. Therefore, a renewed interest in the development of neuraminidase (NA)-specific methods to characterize the glycoprotein and evaluate potential advantages for NA standardization in influenza vaccines has emerged. NA displays sialidase activity by cleaving off the terminal N-acetylneuraminic acid on α-2,3 or α-2,6 sialic acid containing receptors of host cells...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28179042/major-human-milk-oligosaccharides-are-absorbed-into-the-systemic-circulation-after-oral-administration-in-rats
#3
E Vazquez, A Santos-Fandila, R Buck, R Rueda, M Ramirez
Human milk oligosaccharides (HMO) are involved in many biological functions influencing infant health. Although HMO act locally at the intestine, recent evidence has demonstrated that HMO are partially incorporated into the systemic circulation of breast-fed infants. In the last few years, a large amount of research has been conducted using preclinical models to uncover new biological functions of HMO. The aim of this study was to evaluate the absorption and urine excretion of HMO in rats. We administered a single oral dose of the following HMO: 2'-fucosyllactose (2'-FL), 6'-sialyllactose and lacto-N-neotetraose at different concentrations to adult rats...
February 9, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28079170/growth-and-morbidity-of-gambian-infants-are-influenced-by-maternal-milk-oligosaccharides-and-infant-gut-microbiota
#4
Jasmine C C Davis, Zachery T Lewis, Sridevi Krishnan, Robin M Bernstein, Sophie E Moore, Andrew M Prentice, David A Mills, Carlito B Lebrilla, Angela M Zivkovic
Human milk oligosaccharides (HMOs) play an important role in the health of an infant as substrate for beneficial gut bacteria. Little is known about the effects of HMO composition and its changes on the morbidity and growth outcomes of infants living in areas with high infection rates. Mother's HMO composition and infant gut microbiota from 33 Gambian mother/infant pairs at 4, 16, and 20 weeks postpartum were analyzed for relationships between HMOs, microbiota, and infant morbidity and growth. The data indicate that lacto-N-fucopentaose I was associated with decreased infant morbidity, and 3'-sialyllactose was found to be a good indicator of infant weight-for-age...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28069944/soluble-klotho-binds-monosialoganglioside-to-regulate-membrane-microdomains-and-growth-factor-signaling
#5
George Dalton, Sung-Wan An, Saif I Al-Juboori, Nicole Nischan, Joonho Yoon, Evgenia Dobrinskikh, Donald W Hilgemann, Jian Xie, Kate Luby-Phelps, Jennifer J Kohler, Lutz Birnbaumer, Chou-Long Huang
Soluble klotho, the shed ectodomain of the antiaging membrane protein α-klotho, is a pleiotropic endocrine/paracrine factor with no known receptors and poorly understood mechanism of action. Soluble klotho down-regulates growth factor-driven PI3K signaling, contributing to extension of lifespan, cardioprotection, and tumor inhibition. Here we show that soluble klotho binds membrane lipid rafts. Klotho binding to rafts alters lipid organization, decreases membrane's propensity to form large ordered domains for endocytosis, and down-regulates raft-dependent PI3K/Akt signaling...
January 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27936604/microscale-measurements-of-michaelis-menten-constants-of-neuraminidase-with-nanogel-capillary-electrophoresis-for-the-determination-of-sialic-acid-linkage
#6
Srikanth Gattu, Cassandra L Crihfield, Lisa Ann Holland
Phospholipid nanogels enhance the stability and performance of the exoglycosidase enzyme neuraminidase and are used to create a fixed zone of enzyme within a capillary. With nanogels, there is no need to covalently immobilize the enzyme, as it is physically constrained. This enables rapid quantification of Michaelis-Menten constants (KM) for different substrates and ultimately provides a means to quantify the linkage (i.e. 2-3 versus 2-6) of sialic acids. The fixed zone of enzyme is inexpensive and easily positioned in the capillary to support electrophoresis mediated microanalysis using neuraminidase to analyze sialic acid linkages...
December 12, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27775724/nanostructured-glycan-architecture-is-important-in-the-inhibition-of-influenza-a-virus-infection
#7
Seok-Joon Kwon, Dong Hee Na, Jong Hwan Kwak, Marc Douaisi, Fuming Zhang, Eun Ji Park, Jong-Hwan Park, Hana Youn, Chang-Seon Song, Ravi S Kane, Jonathan S Dordick, Kyung Bok Lee, Robert J Linhardt
Rapid change and zoonotic transmission to humans have enhanced the virulence of the influenza A virus (IAV). Neutralizing antibodies fail to provide lasting protection from seasonal epidemics. Furthermore, the effectiveness of anti-influenza neuraminidase inhibitors has declined because of drug resistance. Drugs that can block viral attachment and cell entry independent of antigenic evolution or drug resistance might address these problems. We show that multivalent 6'-sialyllactose-polyamidoamine (6SL-PAMAM) conjugates, when designed to have well-defined ligand valencies and spacings, can effectively inhibit IAV infection...
October 24, 2016: Nature Nanotechnology
https://www.readbyqxmd.com/read/27713728/cell-free-spent-media-obtained-from-bifidobacterium-bifidum-and-bifidobacterium-crudilactis-grown-in-media-supplemented-with-3-sialyllactose-modulate-virulence-gene-expression-in-escherichia-coli-o157-h7-and-salmonella-typhimurium
#8
Pauline Bondue, Sébastien Crèvecoeur, François Brose, Georges Daube, Marie-Christine Seghaye, Mansel W Griffiths, Gisèle LaPointe, Véronique Delcenserie
Complex oligosaccharides from human milk (HMO) possess an antimicrobial activity and can promote the growth of bifidobacteria such as Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis. In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulence effect on several pathogenic bacteria. Whey is rich in complex bovine milk oligosaccharides (BMO) structurally similar to HMO and B. crudilactis, a species of bovine origin, is able to metabolize some of those complex carbohydrates...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27685666/structures-of-the-streptococcus-sanguinis-srpa-binding-region-with-human-sialoglycans-suggest-features-of-the-physiological-ligand
#9
Lioudmila V Loukachevitch, Barbara A Bensing, Hai Yu, Jie Zeng, Xi Chen, Paul M Sullam, T M Iverson
Streptococcus sanguinis is a leading cause of bacterial infective endocarditis, a life-threatening infection of heart valves. S. sanguinis binds to human platelets with high avidity, and this adherence is likely to enhance virulence. Previous studies suggest that a serine-rich repeat adhesin termed SrpA mediates the binding of S. sanguinis to human platelets via its interaction with sialoglycans on the receptor GPIbα. However, in vitro binding assays with SrpA and defined sialoglycans failed to identify specific high-affinity ligands...
October 11, 2016: Biochemistry
https://www.readbyqxmd.com/read/27660754/porcine-milk-oligosaccharides-and-sialic-acid-concentrations-vary-throughout-lactation
#10
Austin T Mudd, Jaime Salcedo, Lindsey S Alexander, Stacey K Johnson, Caitlyn M Getty, Maciej Chichlowski, Brian M Berg, Daniela Barile, Ryan N Dilger
BACKGROUND: Milk oligosaccharides (OSs) are bioactive components known to influence neonatal development. These compounds have specific physiological functions acting as prebiotics, immune system modulators, and enhancing intestine and brain development. OBJECTIVES: The pig is a commonly used model for studying human nutrition, and there is interest in quantifying OS composition of porcine milk across lactation compared with human milk. In this study, we hypothesized that OS and sialic acid (SA) composition of porcine milk would be influenced by stage of lactation...
2016: Frontiers in Nutrition
https://www.readbyqxmd.com/read/27623031/influenza-viral-hemagglutinin-peptide-inhibits-influenza-viral-entry-by-shielding-the-host-receptor
#11
Qing Chen, Ying Guo
Influenza viral infection of the host begins by the attachment of viral hemagglutinin to a cell surface receptor. In the current study, a hemagglutinin fragment peptide library was screened using an H5N1 recombinant pseudotyped viral system. One peptide, designated HA-pep25, showed effective antiviral activity against both human and avian influenza viral strains (IC50 = 12.0-51.0 μM). A mechanistic study demonstrated direct binding between HA-pep25 and sialyllactose, which mimics the host receptor for the influenza virus...
March 11, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27522435/characterization-of-porcine-milk-oligosaccharides-during-early-lactation-and-their-relation-to-the-fecal-microbiome
#12
J Salcedo, S A Frese, D A Mills, D Barile
The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated. Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans. The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA)...
October 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27367145/it-all-starts-with-a-sandwich-identification-of-sialidases-with-trans-glycosylation-activity
#13
Rune T Nordvang, Christian Nyffenegger, Jesper Holck, Carsten Jers, Birgitte Zeuner, Ulrik K Sundekilde, Anne S Meyer, Jørn D Mikkelsen
Sialidases (3.2.1.18) may exhibit trans-sialidase activity to catalyze sialylation of lactose if the active site topology is congruent with that of the Trypanosoma cruzi trans-sialidase (EC 2.4.1.-). The present work was undertaken to test the hypothesis that a particular aromatic sandwich structure of two amino acids proximal to the active site of the T. cruzi trans-sialidase infers trans-sialidase activity. On this basis, four enzymes with putative trans-sialidase activity were identified through an iterative alignment from 2909 native sialidases available in GenBank, which were cloned and expressed in Escherichia coli...
2016: PloS One
https://www.readbyqxmd.com/read/27338459/temporal-change-of-the-content-of-10-oligosaccharides-in-the-milk-of-chinese-urban-mothers
#14
Sean Austin, Carlos A De Castro, Thierry Bénet, Yangfeng Hou, Henan Sun, Sagar K Thakkar, Gerard Vinyes-Pares, Yumei Zhang, Peiyu Wang
Breastfed infants tend to be less prone to infections and may have improved cognitive benefits compared to formula-fed infants. Human milk oligosaccharides (HMO) are the third most abundant component of human milk, but are absent from formulae. They may be partially responsible for the benefits of breastfeeding. In this cross-sectional observational study, the HMO composition of milk from Chinese mothers was studied to determine the impact of stage of lactation, mode of delivery and geographical location. The content of 10 HMO was measured by HPLC in 446 milk samples from mothers living in three different cities in China...
June 8, 2016: Nutrients
https://www.readbyqxmd.com/read/27334597/crystal-structure-of-raptor-adenovirus-1-fibre-head-and-role-of-the-beta-hairpin-in-siadenovirus-fibre-head-domains
#15
Thanh H Nguyen, Mónika Z Ballmann, Huyen T Do, Hai N Truong, Mária Benkő, Balázs Harrach, Mark J van Raaij
BACKGROUND: Most adenoviruses recognize their host cells via an interaction of their fibre head domains with a primary receptor. The structural framework of adenovirus fibre heads is conserved between the different adenovirus genera for which crystal structures have been determined (Mastadenovirus, Aviadenovirus, Atadenovirus and Siadenovirus), but genus-specific differences have also been observed. The only known siadenovirus fibre head structure, that of turkey adenovirus 3 (TAdV-3), revealed a twisted beta-sandwich resembling the reovirus fibre head architecture more than that of other adenovirus fibre heads, plus a unique beta-hairpin embracing a neighbouring monomer...
2016: Virology Journal
https://www.readbyqxmd.com/read/27306205/multivalent-sialylation-of-%C3%AE-thio-glycoclusters-by-trypanosoma-cruzi-trans-sialidase-and-analysis-by-high-performance-anion-exchange-chromatography
#16
Rosalía Agustí, María Emilia Cano, Alejandro J Cagnoni, José Kovensky, Rosa M de Lederkremer, María Laura Uhrig
The synthesis of multivalent sialylated glycoclusters is herein addressed by a chemoenzymatic approach using the trans-sialidase of Trypanosoma cruzi (TcTS). Multivalent β-thio-galactopyranosides and β-thio-lactosides were used as acceptor substrates and 3'-sialyllactose as the sialic acid donor. High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was shown to be an excellent technique for the analysis of the reaction products. Different eluting conditions were optimized to allow the simultaneous resolution of the sialylated species, as well as their neutral precursors...
October 2016: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27208171/characterization-of-a-high-affinity-sialic-acid-specific-cbm40-from-clostridium-perfringens-and-engineering-of-a-divalent-form
#17
João P Ribeiro, William Pau, Carlo Pifferi, Olivier Renaudet, Annabelle Varrot, Lara K Mahal, Anne Imberty
CBMs (carbohydrate-binding modules) are a class of polypeptides usually associated with carbohydrate-active enzymatic sites. We have characterized a new member of the CBM40 family, coded from a section of the gene NanI from Clostridium perfringens Glycan arrays revealed its preference towards α(2,3)-linked sialosides, which was confirmed and quantified by calorimetric studies. The CBM40 binds to α(2,3)-sialyl-lactose with a Kd of ∼30 μM, the highest affinity value for this class of proteins. Inspired by lectins' structure and their arrangement as multimeric proteins, we have engineered a dimeric form of the CBM, and using SPR (surface plasmon resonance) we have observed 6-11-fold binding increases due to the avidity affect...
July 15, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/26865725/porcine-sapelovirus-uses-%C3%AE-2-3-linked-sialic-acid-on-gd1a-ganglioside-as-a-receptor
#18
Deok-Song Kim, Kyu-Yeol Son, Kyung-Min Koo, Ji-Yun Kim, Mia Madel Alfajaro, Jun-Gyu Park, Myra Hosmillo, Mahmoud Soliman, Yeong-Bin Baek, Eun-Hyo Cho, Ju-Hwan Lee, Mun-Il Kang, Ian Goodfellow, Kyoung-Oh Cho
UNLABELLED: The receptor(s) for porcine sapelovirus (PSV), which causes diarrhea, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs, remains largely unknown. Given the precedent for other picornaviruses which use terminal sialic acids (SAs) as receptors, we examined the role of SAs in PSV binding and infection. Using a variety of approaches, including treating cells with a carbohydrate-destroying chemical (NaIO4), mono- or oligosaccharides (N-acetylneuraminic acid, galactose, and 6'-sialyllactose), linkage-specific sialidases (neuraminidase and sialidase S), lectins (Maakia amurensislectin andSambucus nigralectin), proteases (trypsin and chymotrypsin), and glucosylceramide synthase inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol and phospholipase C), we demonstrated that PSV could recognize α2,3-linked SA on glycolipids as a receptor...
April 2016: Journal of Virology
https://www.readbyqxmd.com/read/26701794/dietary-isomers-of-sialyllactose-increase-ganglioside-sialic-acid-concentrations-in-the-corpus-callosum-and-cerebellum-and-modulate-the-colonic-microbiota-of-formula-fed-piglets
#19
Sheila K Jacobi, Tanya Yatsunenko, Dongpei Li, Somsankar Dasgupta, Robert K Yu, Brian M Berg, Maciej Chichlowski, Jack Odle
BACKGROUND: Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule. Breastfed infants have increased accumulation of ganglioside-bound SA compared with formula-fed infants. OBJECTIVE: This study aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets. METHODS: Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d...
February 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/26697920/different-sialoside-epitopes-on-collagen-film-surfaces-direct-mesenchymal-stem-cell-fate
#20
Antonella Sgambato, Laura Russo, Monica Montesi, Silvia Panseri, Maurilio Marcacci, Elena Caravà, Mario Raspanti, Laura Cipolla
3'-Sialyllactose and 6'-sialyllactose have been covalently linked to collagen films. Preliminary in vitro study on the behavior of mesenchymal stem cells (MSCs) in terms of cell viability, proliferation and induction of osteogenic and chondrogenic related genes has been performed. Results indicate that sialoside epitopes on collagen surface represent a suitable support for MSCs adhesion and cell proliferation, moreover, the neoglycosylation provide MSCs with different and specific stimuli, saccharide-type depending, in term of expression of osteogenic and chondrogenic related genes...
June 22, 2016: ACS Applied Materials & Interfaces
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