Giel Hendriks, Els Adriaens, Ashley Allemang, Julie Clements, Gabrielle Cole, Remco Derr, Maria Engel, Annie Hamel, Darren Kidd, Stephanie Kellum, Tomomi Kiyota, Abby Myhre, Valerie Naëssens, Stefan Pfuhler, Marise Roy, Raja Settivari, Maik Schuler, Andreas Zeller, Jan van Benthem, Philippe Vanparys, David Kirkland
ToxTracker is a mammalian cell reporter assay that predicts the genotoxic properties of compounds with high accuracy. By evaluating induction of various reporter genes that play a key role in relevant cellular pathways, it provides insight into chemical mode-of-action (MoA), thereby supporting discrimination of direct-acting genotoxicants and cytotoxic chemicals. A comprehensive interlaboratory validation trial was conducted, in which the principles outlined in OECD Guidance Document 34 were followed, with the primary objectives of establishing transferability and reproducibility of the assay and confirming the ability of ToxTracker to correctly classify genotoxic and non-genotoxic compounds...
March 28, 2024: Environmental and Molecular Mutagenesis