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fucosyltransferase

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https://www.readbyqxmd.com/read/28711982/correlation-between-the-methylation-of-the-fut1-promoter-region-and-fut1-expression-in-the-duodenum-of-piglets-from-newborn-to-weaning
#1
Chaohui Dai, Li Sun, Riwei Xia, Shouyong Sun, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
Alpha-(1,2)-fucosyltransferase (FUT1) gene has some influence on economically important traits and disease resistance. DNA methylation plays an important role in human diseases but is relatively poorly studied in pigs by regulating the mRNA expression of genes. The aim of this study was to analyze the influence of promoter methylation on the expression of FUT1 gene. We used bisulfite sequencing PCR (BSP) and qPCR to analyze the methylation of the FUT1 5'-flanking region and FUT1 mRNA expression in the duodenum of Sutai piglets from newborn to weaning...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28710295/relationships-between-gastrointestinal-microbiota-and-blood-group-antigens
#2
Anuhya Gampa, Phillip A Engen, Rima Shobar, Ece A Mutlu
FUT2 is a gene for a fucosyltransferase that encodes expression of ABO blood group antigens found on gastrointestinal mucosa and secretions. We hypothesized that the fecal microbiomes of healthy subjects, with blood group antigens A, B and O, have differing compositions. Thirty-three fecal and blood specimens from healthy subjects were analyzed for FUT2 genotype and the fecal microbiome was determined using 454 pyrosequencing. Our data shows that being a blood group secretor is associated with less diversity at higher orders of taxonomy; and the presence of blood group A antigens in the secretor subjects are associated with an expansion families of bacteria within the gut...
July 14, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/28706275/novel-function-of-pregnancy-associated-plasma-protein-a-promotes-endometrium-receptivity-by-up-regulating-n-fucosylation
#3
Ming Yu, Jiao Wang, Shuai Liu, Xiaoqi Wang, Qiu Yan
Glycosylation of uterine endometrial cells plays important roles to determine their receptive function to blastocysts. Trophoblast-derived pregnancy-associated plasma protein A (PAPPA) is specifically elevated in pregnant women serum, and is known to promote trophoblast cell proliferation and adhesion. However, the relationship between PAPPA and endometrium receptivity, as well as the regulation of N-fucosylation remains unclear. We found that rhPAPPA and PAPPA in the serum samples from pregnant women or conditioned medium of trophoblast cells promoted endometrium receptivity in vitro...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28692034/mir-200b-inhibits-proliferation-and-metastasis-of-breast-cancer-by-targeting-fucosyltransferase-iv-and-%C3%AE-1-3-fucosylated-glycans
#4
Q Zheng, X Cui, D Zhang, Y Yang, X Yan, M Liu, B Niang, F Aziz, S Liu, Q Yan, J Liu
Aberrant protein fucosylation is associated with cancer malignancy. Fucosyltransferase IV (FUT4) is the key enzyme catalyzing the biosynthesis of α1,3-linkage fucosylated glycans carried by glycoproteins on the cell surface, such as the tumor-associated sugar antigen Lewis Y (LeY). An abnormal increase in the levels of FUT4 and LeY is observed in many cancers and correlated with cell proliferation and metastasis. Some microRNAs (miRNAs) are known to negatively regulate gene expression. FUT4 is an oncogenic glycogene, and thus it is important to identify the specific miRNA targeting FUT4...
July 10, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28678517/substrate-preference-and-interplay-of-fucosyltransferase%C3%A2-8-and-n-acetylglucosaminyltransferases
#5
Tzu-Hao Tseng, Tzu-Wen Lin, Chien-Yu Chen, Chein-Hung Chen, Jung-Lee Lin, Tsui-Ling Hsu, Chi-Huey Wong
The core fucosylation of N-glycans on glycoproteins is catalyzed by fucosyltransferase 8 (FUT8) in mammalian cells and is involved in various biological functions, such as protein function, cancer progression, and postnatal development. The substrate specificity of FUT8 toward bi-antennary N-glycans has been reported, but it is unclear with regard to tri-antennary and tetra-antennary glycans. Here, we examined the specificity and activity of human FUT8 toward tri- and tetra-antennary N-glycans in the forms of glycopeptides...
July 11, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28670741/structural-mutagenic-and-in-silico-studies-of-xyloglucan-fucosylation-in-arabidopsis-thaliana-suggest-a-water-mediated-mechanism
#6
Breeanna R Urbanowicz, Vivek S Bharadwaj, Markus Alahuhta, Maria J Peña, Vladimir V Lunin, Yannick J Bomble, Shuo Wang, Jeong-Yeh Yang, Sami T Tuomivaara, Michael E Himmel, Kelley W Moremen, William S York, Michael F Crowley
The mechanistic underpinnings of the complex process of plant polysaccharide biosynthesis are poorly understood, largely due to the resistance of glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase-1 (AtFUT1) catalyzes the regiospecific transfer of terminal 1,2-fucosyl residues to xyloglucan side chains - a key step in the biosynthesis of fucosylated sidechains of galactoxyloglucan. We unravel the mechanistic basis for fucosylation by AtFUT1 with a multipronged approach involving protein expression, X-ray crystallography, mutagenesis experiments and molecular simulations...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28642740/multigenerational-influences-of-the-fut2-gene-on-the-dynamics-of-the-gut-microbiota-in-mice
#7
Philipp Rausch, Sven Künzel, Abdulhadi Suwandi, Guntram A Grassl, Philip Rosenstiel, John F Baines
The FUT2 gene encodes an α-1,2-fucosyltransferase responsible for the expression of ABO histo-blood-group antigens on mucosal surfaces and bodily secretions. Individuals who carry at least one functional allele are known as "secretors," whereas those homozygous for loss-of-function mutations are known as "non-secretors." Non-secretor individuals are more susceptible to chronic inflammatory disorders such as Crohn's Disease, which may be mediated by alterations in the microbiota. Here, we investigated the dynamics of microbial community assembly with respect to genotype using a Fut2-deficient mouse model, taking the genotype of the maternal lineage over two generations into account...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28600306/expression-of-lewis-a-glycans-on-polymorphonuclear-leukocytes-augments-function-by-increasing-transmigration
#8
Jennifer C Brazil, Ronen Sumagin, Sean R Stowell, Goo Lee, Nancy A Louis, Richard D Cummings, Charles A Parkos
PMN-expressed fucosylated glycans from the Lewis glycan family, including Lewis-x (Le(x)) and sialyl Lewis-x (sLe(x)), have previously been implicated in the regulation of important PMN functions, including selectin-mediated trafficking across vascular endothelium. Although glycans, such as Le(x) and sLe(x), which are based on the type 2 sequence (Galβ1-4GlcNAc-R), are abundant on PMNs, the presence of type 1 Galβ1-3GlcNAc-R glycans required for PMN expression of the closely related stereoisomer of Le(x), termed Lewis-A (Le(a)), has not, to our knowledge, been reported...
June 9, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28586014/lewis-y-antigen-promotes-the-progression-of-epithelial-ovarian-cancer-by-stimulating-muc1-expression
#9
Rui Hou, Luo Jiang, Dawo Liu, Bei Lin, Zhenhua Hu, Jian Gao, Danye Zhang, Shulan Zhang, Masao Iwamori
MUC1 is a type I transmembrane glycoprotein and is overexpressed in various epithelial tumor tissues. Some researchers have demonstrated that the glycosylation status of MUC1 can affect MUC1-mediated tumor growth and cell differentiation. In our previous study, we proved that the abilities of cell proliferation, adhesion, invasion and metastasis, and drug resistance were enhanced in ovarian cancer cells stably expressing Lewis(y). Therefore, we hypothesized that Lewis(y) antigen may play a central role in regulating MUC1 expression, and MUC1-mediated cell growth and differentiation may be closely associated with Lewis(y) antigen...
June 2, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28533485/high-expression-of-fut3-is-linked-to-poor-prognosis-in-clear-cell-renal-cell-carcinoma
#10
Li Meng, Le Xu, Yuanfeng Yang, Lin Zhou, Yuan Chang, Tianming Shi, Cheng Tan, Huimin An, Yu Zhu, Jiejie Xu
BACKGROUND AND PURPOSE: Some of the fucosylation catalyzed by fucosyltransferase-III mediates the epithelial-mesenchymal transition and enhances tumor cell-macrophage signaling, which promotes malignant transforming and immune evasion. The aim of the study was to investigate the association between the expression of fucosyltransferase-III and clinical outcomes of patients with clear-cell renal cell carcinoma after surgery. RESULTS: High fucosyltransferase-III expression was associated with a greater risk of recurrence (p = 0...
May 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28530709/recognition-of-egf-like-domains-by-the-notch-modifying-o-fucosyltransferase-pofut1
#11
Zhijie Li, Kristina Han, John E Pak, Malathy Satkunarajah, Dongxia Zhou, James M Rini
Protein O-fucosyltransferase 1 (POFUT1) fucosylates the epidermal growth factor (EGF)-like domains found in cell-surface and secreted glycoproteins including Notch and its ligands. Although Notch fucosylation is critical for development, and POFUT1 deficiency leads to human disease, how this enzyme binds and catalyzes the fucosylation of its diverse EGF-like domain substrates has not been determined. Reported here is the X-ray crystal structure of mouse POFUT1 in complex with several EGF-like domains, including EGF12 and EGF26 of Notch...
July 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28483669/mechanisms-of-pulmonary-fibrosis-induced-by-core-fucosylation-in-pericytes
#12
Wei Sun, HaiYing Tang, Lili Gao, Xiuna Sun, Jia Liu, WeiDong Wang, Taihua Wu, Hongli Lin
Pulmonary fibrosis is a common outcome of a variety of pulmonary interstitial diseases, and myofibroblasts are the main culprit for this process. Recent studies have found that pericytes are one of the major sources of myofibroblasts; the transformation of which involves a complex process of activation of TGF-β/Smad2/3 and PDGFβ/Erk signaling pathways. We have reported that the transforming growth factor-β receptor and platelet-derived growth factor-β receptor (TGF-βR I and PDGFβR, respectively) are modified by glycosylation...
July 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28481220/mrna-mediated-glycoengineering-ameliorates-deficient-homing-of-human-stem-cell-derived-hematopoietic-progenitors
#13
Jungmin Lee, Brad Dykstra, Joel A Spencer, Laurie L Kenney, Dale L Greiner, Leonard D Shultz, Michael A Brehm, Charles P Lin, Robert Sackstein, Derrick J Rossi
Generation of functional hematopoietic stem and progenitor cells (HSPCs) from human pluripotent stem cells (PSCs) has been a long-sought-after goal for use in hematopoietic cell production, disease modeling, and eventually transplantation medicine. Homing of HSPCs from bloodstream to bone marrow (BM) is an important aspect of HSPC biology that has remained unaddressed in efforts to derive functional HSPCs from human PSCs. We have therefore examined the BM homing properties of human induced pluripotent stem cell-derived HSPCs (hiPS-HSPCs)...
June 1, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28440416/fuca1-is-induced-by-wild-type-p53-and-expressed-at-different-levels-in-thyroid-cancers-depending-on-p53-status
#14
Nobuo Tsuchida, Masa-Aki Ikeda, Υoshizumu Ιshino, Michele Grieco, Giancarlo Vecchio
Fucose residues of cell surface glycans, which play important roles in growth, invasion and metastasis, are added by fucosyltransferases (FUTs) and removed by α-L-fucosidases (FUCAs). By the differential display method, we isolated a 3' non-coding region of α-L-fucosidase-1 (FUCA1) (a gene coding for the lysosomal fucosidase-1 enzyme) as a wild-type p53-inducible gene: 18S and 20S FUCA1 mRNA species were induced in Saos-2 cells transfected with a temperature-sensitive p53 mutant at the permissive temperature...
April 20, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28430973/biological-functions-of-fucose-in-mammals
#15
Michael Schneider, Esam Al-Shareffi, Robert S Haltiwanger
Fucose is a 6-deoxy hexose in the l-configuration found in a large variety of different organisms. In mammals, fucose is incorporated into N-glycans, O-glycans and glycolipids by 13 fucosyltransferases, all of which utilize the nucleotide-charged form, GDP-fucose, to modify targets. Three of the fucosyltransferases, FUT8, FUT12/POFUT1 and FUT13/POFUT2, are essential for proper development in mice. Fucose modifications have also been implicated in many other biological functions including immunity and cancer...
July 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28423676/tumor-associated-macrophages-promote-ezrin-phosphorylation-mediated-epithelial-mesenchymal-transition-in-lung-adenocarcinoma-through-fut4-ley-up-regulation
#16
Aman Wang, Chang Lu, Zhen Ning, Wei Gao, Yunpeng Xie, Ningning Zhang, Jinxiao Liang, Faisal S Abbasi, Qiu Yan, Jiwei Liu
Tumor-associated macrophages (TAMs) are key components of tumor microenvironment (TME) during tumorigenesis and progression. However, the role of TAMs in lung adenocarcinoma is still unclear. In this study, we aimed to clarify the mechanism underlying the crosstalk between TAMs and epithelial-mesenchymal transition (EMT) of lung adenocarcinoma. Fucosyltransferase IV (FUT4) and its synthetic cancer sugar antigen Lewis Y (LeY) was aberrantly elevated in various solid tumors, it plays critical role in the invasion and metastasis...
April 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28407380/n-glycan-maturation-mutants-in-lotus-japonicus-for-basic-and-applied-glycoprotein-research
#17
Carina T Pedersen, Ian Loke, Andrea Lorentzen, Sara Wolf, Manoj Kamble, Sebastian K Kristensen, David Munch, Simona Radutoiu, Edzard Spillner, Peter Roepstorff, Morten Thaysen-Andersen, Jens Stougaard, Svend Dam
Studies of protein N-glycosylation are important for answering fundamental questions on the diverse functions of glycoproteins in plant growth and development. Here we generated and characterised a comprehensive collection of Lotus japonicusLORE1 insertion mutants, each lacking the activity of one of the 12 enzymes required for normal N-glycan maturation in the glycosylation machinery. The inactivation of the individual genes resulted in altered N-glycan patterns as documented using mass spectrometry and glycan-recognising antibodies, indicating successful identification of null mutations in the target glyco-genes...
August 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28396675/inactivation-of-the-%C3%AE-1-2-xylosyltransferase-and-the-%C3%AE-1-3-fucosyltransferase-genes-in-nicotiana-tabacum-by-2-cells-by-a-multiplex-crispr-cas9-strategy-results-in-glycoproteins-without-plant-specific-glycans
#18
Sébastien Mercx, Nicolas Smargiasso, François Chaumont, Edwin De Pauw, Marc Boutry, Catherine Navarre
Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines. However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein. Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT). Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28371609/trastuzumab-and-pertuzumab-plant-biosimilars-modification-of-asn297-linked-glycan-of-the-mabs-produced-in-a-plant-with-fucosyltransferase-and-xylosyltransferase-gene-knockouts
#19
T V Komarova, E V Sheshukova, E N Kosobokova, M V Serebryakova, V S Kosorukov, V N Tashlitsky, Y L Dorokhov
Plant biosimilars of anticancer therapeutic antibodies are of interest not only because of the prospects of their practical use, but also as an instrument and object for study of plant protein glycosylation. In this work, we first designed a pertuzumab plant biosimilar (PPB) and investigated the composition of its Asn297-linked glycan in comparison with trastuzumab plant biosimilar (TPB). Both biosimilars were produced in wild-type (WT) Nicotiana benthamiana plant (PPB-WT and TPB-WT) and transgenic ΔXTFT N...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28334865/structure-of-human-pofut1-its-requirement-in-ligand-independent-oncogenic-notch-signaling-and-functional-effects-of-dowling-degos-mutations
#20
Brian J McMillan, Brandon Zimmerman, Emily D Egan, Michael Lofgren, Xiang Xu, Anthony Hesser, Stephen C Blacklow
Protein O-fucosyltransferase-1 (POFUT1), which transfers fucose residues to acceptor sites on serine and threonine residues of epidermal growth factor-like repeats of recipient proteins, is essential for Notch signal transduction in mammals. Here, we examine the consequences of POFUT1 loss on the oncogenic signaling associated with certain leukemia-associated mutations of human Notch1, report the structures of human POFUT1 in free and GDP-fucose bound states, and assess the effects of Dowling-Degos mutations on human POFUT1 function...
March 17, 2017: Glycobiology
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