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fucosyltransferase

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https://www.readbyqxmd.com/read/28798691/fentanyl-promotes-breast-cancer-cell-stemness-and-epithelial-mesenchymal-transition-by-upregulating-%C3%AE-1-6-fucosylation-via-wnt-%C3%AE-catenin-signaling-pathway
#1
Hong-Fang Yang, Ming Yu, Hui-Dan Jin, Jia-Qi Yao, Zhi-Li Lu, Iddrisu B Yabasin, Qiu Yan, Qing-Ping Wen
Cancer pain is a common and severe complication of human breast cancer, and relieving pain is fundamental strategy in the treatment. Fentanyl, as an opioid analgesic, is widely used in breast cancer patients. However, little is known about its effects on stemness and epithelial-mesenchymal transition (EMT) of breast cancer cells. Aberrant protein glycosylation is involved in cancer malignancy. The α1, 6-fucosylation is an important type of glycosylation, and the elevated α1, 6-fucosylation catalyzed by fucosyltransferase VIII (FUT8) is found in many tumors...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28771224/mir-125a-3p-fut5-fut6-axis-mediates-colorectal-cancer-cell-proliferation-migration-invasion-and-pathological-angiogenesis-via-pi3k-akt-pathway
#2
Leilei Liang, Chengshun Gao, Yang Li, Mingming Sun, Jingchao Xu, Huairui Li, Li Jia, Yongfu Zhao
The fucosyltransferase (FUT) family produces glycans, a fundamental event in several cancers, including colorectal cancer (CRC). miR-125a-3p is a non-coding RNA that can reduce cell proliferation and migration in cancer. In this study, we explored the levels of miR-125a-3p and FUT expression in human CRC tissues and two human CRC cell lines by qPCR. The results showed that miR-125a-3p, FUT5 and FUT6 are differentially expressed in normal and tumour tissues. On the basis of our previous research, FUT can be regulated by miRNA, which influences the proliferation and invasion of breast and hepatocellular cancer cells...
August 3, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28768188/blockage-of-core-fucosylation-reduces-cell-surface-expression-of-pd-1-and-promotes-anti-tumor-immune-responses-of-t-cells
#3
Masahiro Okada, Shunsuke Chikuma, Taisuke Kondo, Sana Hibino, Hiroaki Machiyama, Tadashi Yokosuka, Miyako Nakano, Akihiko Yoshimura
Programmed cell death 1 (PD-1) is highly expressed on exhausted T cells and inhibits T cell activation. Antibodies that block the interaction between PD-1 and its ligand prevent this inhibitory signal and reverse T cell dysfunction, providing beneficial anti-tumor responses in a substantial number of patients. Mechanisms for the induction and maintenance of high PD-1 expression on exhausted T cells have not been fully understood. Utilizing a genome-wide loss-of-function screening method based on the CRISPR-Cas9 system, we identified genes involved in the core fucosylation pathway as positive regulators of cell-surface PD-1 expression...
August 1, 2017: Cell Reports
https://www.readbyqxmd.com/read/28764968/production-of-human-milk-oligosaccharides-by-enzymatic-and-whole-cell-microbial-biotransformations
#4
Georg A Sprenger, Florian Baumgärtner, Christoph Albermann
Human milk oligosaccharides (HMO) are almost unique constituents of breast milk and are not found in appreciable amounts in cow milk. Due to several positive aspects of HMO for the development, health, and wellbeing of infants, production of HMO would be desirable. As a result, scientist from different disciplines have developed methods for the preparation of single HMO compounds. Here, we review approaches to HMO preparation by (chemo-)enzymatic syntheses or by whole-cell biotransformation with recombinant bacterial cells...
July 29, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28753226/golgi-%C3%AE-1-4-fucosyltransferase-of-arabidopsis-thaliana-partially-localizes-at-the-nuclear-envelope
#5
Stephan Rips, Manuel Frank, Annegret Elting, Jan Niklas Offenborn, Antje von Schaewen
We analyzed plant-derived α1,4-Fucosyltransferase (FucTc) homologs by reporter fusions and focused on representatives of the Brassicaceae and Solanaceae. Arabidopsis thaliana AtFucTc-GFP or tomato LeFucTc-GFP restored Lewis-a formation in a fuctc mutant, confirming functionality in the trans-Golgi. AtFucTc-GFP partly accumulated at the nuclear envelope (NE) not observed for other homologs or truncated AtFucTc lacking the N-terminus or catalytic domain. Analysis of At/LeFucTc-GFP swap constructs with exchanged cytosolic, transmembrane and stalk (CTS), or only the CT regions, revealed that sorting information resides in the membrane anchor...
July 28, 2017: Traffic
https://www.readbyqxmd.com/read/28731163/-corrigendum-fuca1-is-induced-by-wild-type-p53-and-expressed-at-different-levels-in-thyroid-cancers-depending-on-p53-status
#6
Nobuo Tsuchida, Masa-Aki Ikeda, Yoshizumu Ishino, Michele Grieco, Giancarlo Vecchio
Subsequent to the publication of the above paper, the authors noted that there was an error on Fig. 4 of page 2046, in which the symbols of α-L-fucosidase 1 (FUCA1) and fucosyltransferase 8 (FUT8) had been mistakenly labelled the wrong way around (FUCA1 should have been represented by the blue bars, and FUT8 by the red). The corrected version of Fig. 4 is shown below, which now accurately shows how average levels of FUCA1 mRNA were low in the anaplastic thyroid cancer samples (ATCs), whereas expression levels in the papillary thyroid cancer samples (PTCs) were comparable, or slightly higher, than those present in normal thyroid tissues...
July 21, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28729422/o-glycosylation-modulates-the-stability-of-epidermal-growth-factor-like-repeats-and-thereby-regulates-notch-trafficking
#7
Hideyuki Takeuchi, Hongjun Yu, Huilin Hao, Megumi Takeuchi, Atsuko Ito, Huilin Li, Robert S Haltiwanger
Glycosylation in the endoplasmic reticulum (ER) is closely associated with protein folding and quality control. We recently described a non-canonical ER quality control mechanism for folding of thrombospondin type 1 repeats by Protein O-fucosyltransferase 2 (POFUT2). Epidermal Growth Factor-like (EGF) repeats are also small, cysteine rich protein motifs that can be O-glycosylated by several ER-localized enzymes including Protein O-glucosyltransferase 1 (POGLUT1) and POFUT1. Both POGLUT1 and POFUT1 modify the Notch receptor on multiple EGF repeats and are essential for full Notch function...
July 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28729420/revisiting-the-substrate-specificity-of-mammalian-%C3%AE-1-6-fucosyltransferase-fut8-reveals-that-it-catalyzes-core-fucosylation-of-n-glycans-lacking-%C3%AE-1-3-arm-glcnac
#8
Qiang Yang, Roushu Zhang, Hui Cai, Lai-Xi Wang
The mammalian α1,6-fucosyltransferase (FUT8) catalyzes the core fucosylation of N-glycans in the biosynthesis of glycoproteins. Previously, intensive in vitro studies with crude extract or purified enzyme concluded that the attachment of a GlcNAc on the α1,3 mannose arm of N-glycan is essential for FUT8 catalyzed core fucosylation. In contrast, we have recently shown that expression of erythropoietin (EPO) in a GnTI-knockout, FUT8 overexpressing cell line results in the production of fully core-fucosylated glycoforms of the oligomannose substrate Man5GlcNAc2, suggesting that FUT8 can catalyze core fucosylation of N-glycans lacking an α1,3arm GlcNAc in cells...
July 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28711982/correlation-between-the-methylation-of-the-fut1-promoter-region-and-fut1-expression-in-the-duodenum-of-piglets-from-newborn-to-weaning
#9
Chaohui Dai, Li Sun, Riwei Xia, Shouyong Sun, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
Alpha-(1,2)-fucosyltransferase (FUT1) gene has some influence on economically important traits and disease resistance. DNA methylation plays an important role in human diseases but is relatively poorly studied in pigs by regulating the mRNA expression of genes. The aim of this study was to analyze the influence of promoter methylation on the expression of FUT1 gene. We used bisulfite sequencing PCR (BSP) and qPCR to analyze the methylation of the FUT1 5'-flanking region and FUT1 mRNA expression in the duodenum of Sutai piglets from newborn to weaning...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28710295/relationships-between-gastrointestinal-microbiota-and-blood-group-antigens
#10
Anuhya Gampa, Phillip A Engen, Rima Shobar, Ece A Mutlu
FUT2 is a gene for a fucosyltransferase that encodes expression of ABO blood group antigens found on gastrointestinal mucosa and secretions. We hypothesized that the fecal microbiomes of healthy subjects, with blood group antigens A, B and O, have differing compositions. Thirty-three fecal and blood specimens from healthy subjects were analyzed for FUT2 genotype and the fecal microbiome was determined using 454 pyrosequencing. Our data shows that being a blood group secretor is associated with less diversity at higher orders of taxonomy; and the presence of blood group A antigens in the secretor subjects are associated with an expansion families of bacteria within the gut...
July 14, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/28706275/novel-function-of-pregnancy-associated-plasma-protein-a-promotes-endometrium-receptivity-by-up-regulating-n-fucosylation
#11
Ming Yu, Jiao Wang, Shuai Liu, Xiaoqi Wang, Qiu Yan
Glycosylation of uterine endometrial cells plays important roles to determine their receptive function to blastocysts. Trophoblast-derived pregnancy-associated plasma protein A (PAPPA) is specifically elevated in pregnant women serum, and is known to promote trophoblast cell proliferation and adhesion. However, the relationship between PAPPA and endometrium receptivity, as well as the regulation of N-fucosylation remains unclear. We found that rhPAPPA and PAPPA in the serum samples from pregnant women or conditioned medium of trophoblast cells promoted endometrium receptivity in vitro...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28692034/mir-200b-inhibits-proliferation-and-metastasis-of-breast-cancer-by-targeting-fucosyltransferase-iv-and-%C3%AE-1-3-fucosylated-glycans
#12
Q Zheng, X Cui, D Zhang, Y Yang, X Yan, M Liu, B Niang, F Aziz, S Liu, Q Yan, J Liu
Aberrant protein fucosylation is associated with cancer malignancy. Fucosyltransferase IV (FUT4) is the key enzyme catalyzing the biosynthesis of α1,3-linkage fucosylated glycans carried by glycoproteins on the cell surface, such as the tumor-associated sugar antigen Lewis Y (LeY). An abnormal increase in the levels of FUT4 and LeY is observed in many cancers and correlated with cell proliferation and metastasis. Some microRNAs (miRNAs) are known to negatively regulate gene expression. FUT4 is an oncogenic glycogene, and thus it is important to identify the specific miRNA targeting FUT4...
July 10, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28678517/substrate-preference-and-interplay-of-fucosyltransferase%C3%A2-8-and-n-acetylglucosaminyltransferases
#13
Tzu-Hao Tseng, Tzu-Wen Lin, Chien-Yu Chen, Chein-Hung Chen, Jung-Lee Lin, Tsui-Ling Hsu, Chi-Huey Wong
The core fucosylation of N-glycans on glycoproteins is catalyzed by fucosyltransferase 8 (FUT8) in mammalian cells and is involved in various biological functions, such as protein function, cancer progression, and postnatal development. The substrate specificity of FUT8 toward bi-antennary N-glycans has been reported, but it is unclear with regard to tri-antennary and tetra-antennary glycans. Here, we examined the specificity and activity of human FUT8 toward tri- and tetra-antennary N-glycans in the forms of glycopeptides...
July 11, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28670741/structural-mutagenic-and-in-silico-studies-of-xyloglucan-fucosylation-in-arabidopsis-thaliana-suggest-a-water-mediated-mechanism
#14
Breeanna R Urbanowicz, Vivek S Bharadwaj, Markus Alahuhta, Maria J Peña, Vladimir V Lunin, Yannick J Bomble, Shuo Wang, Jeong-Yeh Yang, Sami T Tuomivaara, Michael E Himmel, Kelley W Moremen, William S York, Michael F Crowley
The mechanistic underpinnings of the complex process of plant polysaccharide biosynthesis are poorly understood, largely due to the resistance of glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase-1 (AtFUT1) catalyzes the regiospecific transfer of terminal 1,2-fucosyl residues to xyloglucan side chains - a key step in the biosynthesis of fucosylated sidechains of galactoxyloglucan. We unravel the mechanistic basis for fucosylation by AtFUT1 with a multipronged approach involving protein expression, X-ray crystallography, mutagenesis experiments and molecular simulations...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28642740/multigenerational-influences-of-the-fut2-gene-on-the-dynamics-of-the-gut-microbiota-in-mice
#15
Philipp Rausch, Sven Künzel, Abdulhadi Suwandi, Guntram A Grassl, Philip Rosenstiel, John F Baines
The FUT2 gene encodes an α-1,2-fucosyltransferase responsible for the expression of ABO histo-blood-group antigens on mucosal surfaces and bodily secretions. Individuals who carry at least one functional allele are known as "secretors," whereas those homozygous for loss-of-function mutations are known as "non-secretors." Non-secretor individuals are more susceptible to chronic inflammatory disorders such as Crohn's Disease, which may be mediated by alterations in the microbiota. Here, we investigated the dynamics of microbial community assembly with respect to genotype using a Fut2-deficient mouse model, taking the genotype of the maternal lineage over two generations into account...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28600306/expression-of-lewis-a-glycans-on-polymorphonuclear-leukocytes-augments-function-by-increasing-transmigration
#16
Jennifer C Brazil, Ronen Sumagin, Sean R Stowell, Goo Lee, Nancy A Louis, Richard D Cummings, Charles A Parkos
PMN-expressed fucosylated glycans from the Lewis glycan family, including Lewis-x (Le(x)) and sialyl Lewis-x (sLe(x)), have previously been implicated in the regulation of important PMN functions, including selectin-mediated trafficking across vascular endothelium. Although glycans, such as Le(x) and sLe(x), which are based on the type 2 sequence (Galβ1-4GlcNAc-R), are abundant on PMNs, the presence of type 1 Galβ1-3GlcNAc-R glycans required for PMN expression of the closely related stereoisomer of Le(x), termed Lewis-A (Le(a)), has not, to our knowledge, been reported...
June 9, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28586014/lewis-y-antigen-promotes-the-progression-of-epithelial-ovarian-cancer-by-stimulating-muc1-expression
#17
Rui Hou, Luo Jiang, Dawo Liu, Bei Lin, Zhenhua Hu, Jian Gao, Danye Zhang, Shulan Zhang, Masao Iwamori
MUC1 is a type I transmembrane glycoprotein and is overexpressed in various epithelial tumor tissues. Some researchers have demonstrated that the glycosylation status of MUC1 can affect MUC1-mediated tumor growth and cell differentiation. In our previous study, we proved that the abilities of cell proliferation, adhesion, invasion and metastasis, and drug resistance were enhanced in ovarian cancer cells stably expressing Lewis(y). Therefore, we hypothesized that Lewis(y) antigen may play a central role in regulating MUC1 expression, and MUC1-mediated cell growth and differentiation may be closely associated with Lewis(y) antigen...
June 2, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28533485/high-expression-of-fut3-is-linked-to-poor-prognosis-in-clear-cell-renal-cell-carcinoma
#18
Li Meng, Le Xu, Yuanfeng Yang, Lin Zhou, Yuan Chang, Tianming Shi, Cheng Tan, Huimin An, Yu Zhu, Jiejie Xu
BACKGROUND AND PURPOSE: Some of the fucosylation catalyzed by fucosyltransferase-III mediates the epithelial-mesenchymal transition and enhances tumor cell-macrophage signaling, which promotes malignant transforming and immune evasion. The aim of the study was to investigate the association between the expression of fucosyltransferase-III and clinical outcomes of patients with clear-cell renal cell carcinoma after surgery. RESULTS: High fucosyltransferase-III expression was associated with a greater risk of recurrence (p = 0...
May 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28530709/recognition-of-egf-like-domains-by-the-notch-modifying-o-fucosyltransferase-pofut1
#19
Zhijie Li, Kristina Han, John E Pak, Malathy Satkunarajah, Dongxia Zhou, James M Rini
Protein O-fucosyltransferase 1 (POFUT1) fucosylates the epidermal growth factor (EGF)-like domains found in cell-surface and secreted glycoproteins including Notch and its ligands. Although Notch fucosylation is critical for development, and POFUT1 deficiency leads to human disease, how this enzyme binds and catalyzes the fucosylation of its diverse EGF-like domain substrates has not been determined. Reported here is the X-ray crystal structure of mouse POFUT1 in complex with several EGF-like domains, including EGF12 and EGF26 of Notch...
July 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28483669/mechanisms-of-pulmonary-fibrosis-induced-by-core-fucosylation-in-pericytes
#20
Wei Sun, HaiYing Tang, Lili Gao, Xiuna Sun, Jia Liu, WeiDong Wang, Taihua Wu, Hongli Lin
Pulmonary fibrosis is a common outcome of a variety of pulmonary interstitial diseases, and myofibroblasts are the main culprit for this process. Recent studies have found that pericytes are one of the major sources of myofibroblasts; the transformation of which involves a complex process of activation of TGF-β/Smad2/3 and PDGFβ/Erk signaling pathways. We have reported that the transforming growth factor-β receptor and platelet-derived growth factor-β receptor (TGF-βR I and PDGFβR, respectively) are modified by glycosylation...
July 2017: International Journal of Biochemistry & Cell Biology
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