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fucosyltransferase

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https://www.readbyqxmd.com/read/29138114/the-%C3%AE-1-3-fucosyltransferase-fut7-regulates-il-1%C3%AE-induced-monocyte-endothelial-adhesion-via-fucosylation-of-endomucin
#1
Jun Zhang, Nana Ju, Xi Yang, Linmu Chen, Chao Yu
Monocyte-endothelial adhesion is a hallmark feature of atherosclerosis at early stage and emerging evidence suggests that the glycosylation of vascular adhesive molecules and its ligands is involved in this process. Nevertheless, the mechanism underlying this process remains incompletely elucidated. In this study, we reported that treatment with inflammatory factors interleukin-1β (IL-1β) pronouncedly upregulated α1,3-fucosyltransferase VII gene (FUT7) mRNA and protein expression level in EA.hy926 endothelial cells...
November 11, 2017: Life Sciences
https://www.readbyqxmd.com/read/29130097/c-fos-mediates-%C3%AE-1-2-fucosyltransferase-1-and-lewis%C3%A2-y-expression-in-response-to-tgf-%C3%AE-1-in-ovarian-cancer
#2
Yingying Hao, Liancheng Zhu, Limei Yan, Juanjuan Liu, Dawo Liu, Na Gao, Mingzi Tan, Song Gao, Bei Lin
FUT1 is a key rate-limiting enzyme in the synthesis of Lewis y, a membrane-associated carbohydrate antigen. The aberrant upregulation of FUT1 and Lewis y antigen is related to proliferation, invasion and prognosis in malignant epithelial tumors. A c-Fos/activator protein-1 (AP-1) binding site was found in the FUT1 promoter. However, the mechanisms of transcriptional regulation of FUT1 remain poorly understood. TGF-β1 is positively correlated to Lewis y. In the present study, we investigated the molecular mechanism of FUT1 gene expression in response to TGF-β1...
December 2017: Oncology Reports
https://www.readbyqxmd.com/read/29104485/cell-recognition-molecule-l1-regulates-cell-surface-glycosylation-to-modulate-cell-survival-and-migration
#3
Gang Shi, Yue Du, Yali Li, Yue An, Zhenwei He, Yingwei Lin, Rui Zhang, Xiaofei Yan, Jianfeng Zhao, Shihua Yang, Pang Nghee Kheem Brendan, Fang Liu
Background: Cell recognition molecule L1 (L1) plays an important role in cancer cell differentiation, proliferation, migration and survival, but its mechanism remains unclear. Methodology/Principal: Our previous study has demonstrated that L1 enhanced cell survival and migration in neural cells by regulating cell surface glycosylation. In the present study, we show that L1 affected cell migration and survival in CHO (Chinese hamster ovary) cell line by modulation of sialylation and fucosylation at the cell surface via the PI3K (phosphoinositide 3-kinase) and Erk (extracellularsignal-regulated kinase) signaling pathways...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29095923/pofut1-point-mutations-that-disrupt-o-fucosyltransferase-activity-destabilize-the-protein-and-abolish-notch1-signaling-during-mouse-somitogenesis
#4
Rieko Ajima, Emiko Suzuki, Yumiko Saga
The segmental pattern of the vertebrate body is established via the periodic formation of somites from the presomitic mesoderm (PSM). This periodical process is controlled by the cyclic and synchronized activation of Notch signaling in the PSM. Protein O-fucosyltransferase1 (Pofut1), which transfers O-fucose to the EGF domains of the Notch1 receptor, is indispensable for Notch signaling activation. The Drosophila homologue Ofut1 was reported to control Notch localization via two different mechanisms, working as a chaperone for Notch or as a regulator of Notch endocytosis...
2017: PloS One
https://www.readbyqxmd.com/read/29079498/infection-s-sweet-tooth-how-glycans-mediate-infection-and-disease-susceptibility
#5
REVIEW
Steven L Taylor, Michael A McGuckin, Steve Wesselingh, Geraint B Rogers
Glycans form a highly variable constituent of our mucosal surfaces and profoundly affect our susceptibility to infection and disease. The diversity and importance of these surface glycans can be seen in individuals who lack a functional copy of the fucosyltransferase gene, FUT2. Representing around one-fifth of the population, these individuals have an altered susceptibility to many bacterial and viral infections and diseases. The mediation of host-pathogen interactions by mucosal glycans, such as those added by FUT2, is poorly understood...
October 24, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28990779/designer-%C3%AE-1-6-fucosidase-mutants-enable-direct-core-fucosylation-of-intact-n-glycopeptides-and-n-glycoproteins
#6
Chao Li, Shilei Zhu, Christopher Ma, Lai-Xi Wang
Core fucosylation of N-glycoproteins plays a crucial role in modulating the biological functions of glycoproteins. Yet, the synthesis of structurally well-defined, core-fucosylated glycoproteins remains a challenging task due to the complexity in multistep chemical synthesis or the inability of the biosynthetic α1,6-fucosyltransferase (FUT8) to directly fucosylate full-size mature N-glycans in a chemoenzymatic approach. We report in this paper the design and generation of potential α1,6-fucosynthase and fucoligase for direct core fucosylation of intact N-glycoproteins...
October 25, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28988527/how-fucose-of-blood-group-glycotopes-programs-human-gut-microbiota
#7
REVIEW
S V Kononova
Formation of appropriate gut microbiota is essential for human health. The first two years of life is the critical period for this process. Selection of mutualistic microorganisms of the intestinal microbiota is controlled by the FUT2 and FUT3 genes that encode fucosyltransferases, enzymes responsible for the synthesis of fucosylated glycan structures of mucins and milk oligosaccharides. In this review, the mechanisms of the selection and maintenance of intestinal microorganisms that involve fucosylated oligosaccharides of breast milk and mucins of the newborn's intestine are described...
September 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28982386/fut8-promotes-breast-cancer-cell-invasiveness-by-remodeling-tgf-%C3%AE-receptor-core-fucosylation
#8
Cheng-Fen Tu, Meng-Ying Wu, Yuh-Charn Lin, Reiji Kannagi, Ruey-Bing Yang
BACKGROUND: Core fucosylation (addition of fucose in α-1,6-linkage to core N-acetylglucosamine of N-glycans) catalyzed by fucosyltransferase 8 (FUT8) is critical for signaling receptors involved in many physiological and pathological processes such as cell growth, adhesion, and tumor metastasis. Transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition (EMT) regulates the invasion and metastasis of breast tumors. However, whether receptor core fucosylation affects TGF-β signaling during breast cancer progression remains largely unknown...
October 5, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28977844/high-expression-of-fut3-is-linked-to-poor-prognosis-in-clear-cell-renal-cell-carcinoma
#9
Li Meng, Le Xu, Yuanfeng Yang, Lin Zhou, Yuan Chang, Tianming Shi, Cheng Tan, Huimin An, Yu Zhu, Jiejie Xu
BACKGROUND AND PURPOSE: Some of the fucosylation catalyzed by fucosyltransferase-III mediates the epithelial-mesenchymal transition and enhances tumor cell-macrophage signaling, which promotes malignant transforming and immune evasion. The aim of the study was to investigate the association between the expression of fucosyltransferase-III and clinical outcomes of patients with clear-cell renal cell carcinoma after surgery. RESULTS: High fucosyltransferase-III expression was associated with a greater risk of recurrence (p = 0...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28976642/enhanced-genome-editing-tools-for-multi-gene-deletion-knock-out-approaches-using-paired-crispr-sgrnas-in-cho-cells
#10
Valerie Schmieder, Nina Bydlinski, Richard Strasser, Martina Baumann, Helene Faustrup Kildegaard, Vaibhav Jadhav, Nicole Borth
Since the establishment of clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9, powerful strategies for engineering of CHO cell lines have emerged. Nevertheless, there is still room to expand the scope of the CRISPR tool box for further applications to improve CHO cell factories. Here, we demonstrate activity of the alternative CRISPR endonuclease Cpf1 in CHO-K1 for the first time and that it can be used in parallel to CRISPR/Cas9 without any interference. Both, Cas9 and Cpf1, can be effectively used for multi-gene engineering with a strategy based on paired single guide RNAs (sgRNAs) for full gene deletions...
October 4, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28973293/nf%C3%AE%C2%BAb-mediated-activation-of-the-cellular-fut3-5-and-6-gene-cluster-by-herpes-simplex-virus-type-1
#11
Rickard Nordén, Ebba Samuelsson, Kristina Nyström
Herpes simplex virus type 1 has the ability to induce expression of a human gene cluster located on chromosome 19 upon infection. This gene cluster contains three fucosyltransferases (encoded by FUT3, FUT5 and FUT6) with the ability to add a fucose to an N-acetylglucosamine residue. Little is known regarding the transcriptional activation of these three genes in human cells. Intriguingly, herpes simplex virus type 1 activates all three genes simultaneously during infection, a situation not observed in uninfected tissue, pointing towards a virus specific mechanism for transcriptional activation...
September 4, 2017: Glycobiology
https://www.readbyqxmd.com/read/28973141/core-fucose-is-critical-for-cd14-dependent-toll-like-receptor-4-signaling
#12
Junko Iijima, Satoshi Kobayashi, Shinobu Kitazume, Yasuhiko Kizuka, Reiko Fujinawa, Hiroaki Korekane, Takuma Shibata, Shin-Ichiroh Saitoh, Sachiko Akashi-Takamura, Kensuke Miyake, Eiji Miyoshi, Naoyuki Taniguchi
Core fucosylation, a post-translational modification of N-glycans, modifies several growth factor receptors and impacts on their ligand binding affinity. Core-fucose-deficient mice generated by ablating the α1,6 fucosyltransferase enzyme, Fut8, exhibit severe pulmonary emphysema, partly due to impaired macrophage function, similar to aged Toll-like receptor 4 (Tlr4) -deficient mice. We therefore suspect that a lack of core fucose affects the TLR4-dependent signaling pathway. Indeed, upon lipopolysaccharide stimulation, Fut8-deficient mouse embryonic fibroblasts (MEFs) produced similar levels of interleukin-6 but markedly reduced levels of interferon-β (IFN-β) compared with wild-type MEFs...
August 29, 2017: Glycobiology
https://www.readbyqxmd.com/read/28953003/vitamin-b12-deficiency-in-inflammatory-bowel-disease-a-prospective-observational-pilot-study
#13
Robert Battat, Uri Kopylov, Joshua Byer, Maida J Sewitch, Elham Rahme, Hacene Nedjar, Elana Zelikovic, Serge Dionne, Talat Bessissow, Waqqas Afif, Paula J Waters, Ernest Seidman, Alain Bitton
BACKGROUND AND AIM: Diagnostic and management guidelines for vitamin B12 (cobalamin, Cbl) deficiency in inflammatory bowel disease (IBD) are lacking. True deficiency is defined as Cbl concentrations below reference range combined with elevated methylmalonic acid (MMA) concentrations. Studies analyzing Cbl status in IBD use only Cbl concentrations without confirmatory MMA. This study aims to determine the proportion of IBD patients with Cbl concentrations below reference range and their predisposing clinical and genetic characteristics...
September 25, 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28940895/mir-339-5p-downregulation-contributes-to-taxol-resistance-in-small-cell-lung-cancer-by-targeting-%C3%AE-1-2-fucosyltransferase-1
#14
Chong-Zhi Gan, Gang Li, Qing-Song Luo, Hong-Min Li
Lung cancer is a leading cause of cancer-related mortality, and non-small-cell lung carcinoma is responsible for almost 80% of lung cancer-related deaths. In recent years, lung cancer has shown increasing incidence but poor prognosis, and many studies have demonstrated that microRNAs play crucial roles in the development of lung carcinoma and chemoresistance. This study investigated the role of miR-339-5p involvement in lung carcinoma cell lines and chemoresistance to Taxol. We observed that miR-339-5p was significantly downregulated in Taxol-A549 cells compared with A549 cells...
November 2017: IUBMB Life
https://www.readbyqxmd.com/read/28936496/h-pylori-%C3%AE-1-3-4-fucosyltransferase-hp3-4ft-catalyzed-one-pot-multienzyme-opme-synthesis-of-lewis-antigens-and-human-milk-fucosides
#15
Hai Yu, Yanhong Li, Zhigang Wu, Lei Li, Jie Zeng, Chao Zhao, Yijing Wu, Nova Tasnima, Jing Wang, Huaide Liu, Madhusudhan Reddy Gadi, Wanyi Guan, Peng G Wang, Xi Chen
Helicobacter pylori α1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L(-1) culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of l-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT)...
October 5, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28926673/combinatorial-genome-and-protein-engineering-yields-monoclonal-antibodies-with-hypergalactosylation-from-cho-cells
#16
Cheng-Yu Chung, Qiong Wang, Shuang Yang, Sean A Ponce, Brian J Kirsch, Hui Zhang, Michael J Betenbaugh
One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution. Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies. However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities. In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms...
December 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28925387/a-vital-sugar-code-for-ricin-toxicity
#17
Jasmin Taubenschmid, Johannes Stadlmann, Markus Jost, Tove Irene Klokk, Cory D Rillahan, Andreas Leibbrandt, Karl Mechtler, James C Paulson, Julian Jude, Johannes Zuber, Kirsten Sandvig, Ulrich Elling, Thorsten Marquardt, Christian Thiel, Christian Koerner, Josef M Penninger
Ricin is one of the most feared bioweapons in the world due to its extreme toxicity and easy access. Since no antidote exists, it is of paramount importance to identify the pathways underlying ricin toxicity. Here, we demonstrate that the Golgi GDP-fucose transporter Slc35c1 and fucosyltransferase Fut9 are key regulators of ricin toxicity. Genetic and pharmacological inhibition of fucosylation renders diverse cell types resistant to ricin via deregulated intracellular trafficking. Importantly, cells from a patient with SLC35C1 deficiency are also resistant to ricin...
November 2017: Cell Research
https://www.readbyqxmd.com/read/28916755/protein-o-fucosylation-in-plasmodium-falciparum-ensures-efficient-infection-of-mosquito-and-vertebrate-hosts
#18
Sash Lopaticki, Annie S P Yang, Alan John, Nichollas E Scott, James P Lingford, Matthew T O'Neill, Sara M Erickson, Nicole C McKenzie, Charlie Jennison, Lachlan W Whitehead, Donna N Douglas, Norman M Kneteman, Ethan D Goddard-Borger, Justin A Boddey
O-glycosylation of the Plasmodium sporozoite surface proteins CSP and TRAP was recently identified, but the role of this modification in the parasite life cycle and its relevance to vaccine design remain unclear. Here, we identify the Plasmodium protein O-fucosyltransferase (POFUT2) responsible for O-glycosylating CSP and TRAP. Genetic disruption of POFUT2 in Plasmodium falciparum results in ookinetes that are attenuated for colonizing the mosquito midgut, an essential step in malaria transmission. Some POFUT2-deficient parasites mature into salivary gland sporozoites although they are impaired for gliding motility, cell traversal, hepatocyte invasion, and production of exoerythrocytic forms in humanized chimeric liver mice...
September 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28914881/microrna-200c-impairs-uterine-receptivity-formation-by-targeting-fut4-and-%C3%AE-1-3-fucosylation
#19
Qin Zheng, Dandan Zhang, Y U Yang, Xinyuan Cui, Jiaqi Sun, Caixia Liang, Huamin Qin, Xuesong Yang, Shuai Liu, Qiu Yan
Successful embryo implantation requires the establishment of a receptive endometrium. Poor endometrial receptivity has generally been considered as a major cause of infertility. Protein glycosylation is associated with many physiological and pathological processes. The fucosylation is catalyzed by the specific fucosyltransferases. Fucosyltransferase IV (FUT4) is the key enzyme for the biosynthesis of α1,3-fucosylated glycans carried by glycoproteins, and the previous studies showed FUT4 expression changed dynamically during perimplantation...
December 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28910607/molecular-cloning-and-functional-expression-of-lewis-type-%C3%AE-1-3-%C3%AE-1-4-fucosyltransferase-cdnas-from-mangifera-indica-l
#20
Takahiro Okada, Hideyuki Ihara, Ritsu Ito, Yoshitaka Ikeda
In higher plants, complex type N-glycans contain characteristic carbohydrate moieties that are not found in mammals. In particular, the attachment of the Lewis a (Le(a)) epitope is currently the only known outer chain elongation that is present in plant N-glycans. Such a modification is of great interest in terms of the biological function of complex type N-glycans in plant species. However, little is known regarding the exact molecular basis underlying their Le(a) expression. In the present study, we cloned two novel Lewis type fucosyltransferases (MiFUT13) from mango fruit, Mangifera indica L...
September 11, 2017: Phytochemistry
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