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fucosyltransferase

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https://www.readbyqxmd.com/read/29345354/lack-of-functional-selectin-ligand-interactions-enhances-innate-immune-resistance-to-systemic-listeria-monocytogenes-infection
#1
Gerard Agbayani, Komal Gurnani, Ahmed Zafer, Subash Sad, Lakshmi Krishnan
Selectin-ligand interactions are important for leukocyte homing and functionality. The roles of selectin-ligand interactions in modulating immunity to intracellular infections are not completely understood. Mice lacking the expression of fucosyltransferase-IV and -VII (Fucosyltransferase-IV and -VII double knockout, FtDKO) exhibit deficient functionality of selectin-ligand interactions. We addressed the kinetics of infection and immunity to Listeria monocytogenes (LM), an intracellular pathogen, in FtDKO mice...
December 27, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29339807/overexpression-of-%C3%AE-1-6-fucosyltransferase-in-the-development-of-castration-resistant-prostate-cancer-cells
#2
Naseruddin Höti, Shuang Yang, Yingwei Hu, Punit Shah, Michael C Haffner, Hui Zhang
Glycosylation is recognized as one of the most common modifications on proteins. Recent studies have shown that aberrant expression of α (1,6) fucosyltransferase (FUT8), which catalyzes the transfer of fucose from GDP-fucose to core-GlcNAc of the N-linked glycoproteins, modulates cellular behavior that could lead to the development of aggressive prostate cancer. While the relationship between the abnormal expression of FUT8 and glycoprotein fucosylation in different prostate cancer cells has been demonstrated, there is no evidence that shows dysregulated fucosylation might be involved in prostate cancer progression from androgen-dependent to castration-resistant prostate cancer...
January 16, 2018: Prostate Cancer and Prostatic Diseases
https://www.readbyqxmd.com/read/29339069/multiple-homologous-genes-knockout-ko-by-crispr-cas9-system-in-rabbit
#3
Huan Liu, Tingting Sui, Di Liu, Tingjun Liu, Mao Chen, Jichao Deng, Yuanyuan Xu, Zhanjun Li
The CRISPR/Cas9 system is a highly efficient and convenient genome editing tool, which has been widely used for single or multiple gene mutation in a variety of organisms. Disruption of multiple homologous genes, which have similar DNA sequences and gene function, is required for the study of the desired phenotype. In this study, to test whether the CRISPR/Cas9 system works on the mutation of multiple homologous genes, a single guide RNA (sgRNA) targeting three fucosyltransferases encoding genes (FUT1, FUT2 and SEC1) was designed...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29323143/optimizing-human-treg-immunotherapy-by-treg-subset-selection-and-e-selectin-ligand-expression
#4
Conor Donnelly, Brad Dykstra, Nandini Mondal, Junning Huang, Belinda J Kaskow, Russell Griffin, Robert Sackstein, Clare Baecher-Allan
While human Tregs hold immense promise for immunotherapy, their biologic variability poses challenges for clinical use. Here, we examined clinically-relevant activities of defined subsets of freshly-isolated and culture-expanded human PBMC-derived Tregs. Unlike highly suppressive but plastic memory Tregs (memTreg), naïve Tregs (nvTreg) exhibited the greatest proliferation, suppressive capacity after stimulation, and Treg lineage fidelity. Yet, unlike memTregs, nvTregs lack Fucosyltransferase VII and display low sLeX expression, with concomitant poor homing capacity...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29304374/biallelic-mutations-in-fut8-cause-a-congenital-disorder-of-glycosylation-with-defective-fucosylation
#5
Bobby G Ng, Gege Xu, Nandini Chandy, Joan Steyermark, Deepali N Shinde, Kelly Radtke, Kimiyo Raymond, Carlito B Lebrilla, Ali AlAsmari, Sharon F Suchy, Zöe Powis, Eissa Ali Faqeih, Susan A Berry, David F Kronn, Hudson H Freeze
Fucosyltransferase 8 (FUT8) encodes a Golgi-localized α1,6 fucosyltransferase that is essential for transferring the monosaccharide fucose into N-linked glycoproteins, a process known as "core fucosylation." Here we describe three unrelated individuals, who presented with intrauterine growth retardation, severe developmental and growth delays with shortened limbs, neurological impairments, and respiratory complications. Each underwent whole-exome sequencing and was found to carry pathogenic variants in FUT8...
January 4, 2018: American Journal of Human Genetics
https://www.readbyqxmd.com/read/29304015/development-of-a-surrogate-neutralization-assay-for-norovirus-vaccine-evaluation-at-the-cellular-level
#6
Xiaoli Wang, Shuxia Wang, Chao Zhang, Yu Zhou, Pei Xiong, Qingwei Liu, Zhong Huang
Noroviruses (NoVs) are the main pathogens responsible for sporadic and epidemic nonbacterial gastroenteritis, causing an estimated 219,000 deaths annually worldwide. There is no commercially available vaccine for NoVs, due partly to the difficulty in establishing NoV cell culture models. The histo-blood group antigen (HBGA) blocking assay is used extensively to assess the protective potential of candidate vaccine-elicited antibodies, but there is still no widely used cellular evaluation model. In this study, we have established a cell line-based NoV vaccine evaluation model through the construction of human α1,2-fucosyltransferase 2-overexpressing 293T (293T-FUT2) cell lines...
January 5, 2018: Viruses
https://www.readbyqxmd.com/read/29278651/ap1-mediates-upa-upar-induced-fut4-expression-and-trophoblast-invasion
#7
Qin Zheng, Yu Yang, Xinyuan Cui, Dandan Zhang, Shuai Liu, Qiu Yan
Placental trophoblast invasion is crucial for embryo implantation and successful pregnancy. Urokinase-type plasminogen activator (uPA)/urokinase-type plasminogen activator receptor (uPAR) are expressed on trophoblasts and involved in trophoblast invasion. The transcription factor activator protein 1 (AP-1) (c-FOS and c-JUN) and fucosyltransferase IV (FUT4) have been found to be involved in trophoblast invasion. However, the relationship of uPA/uPAR, AP1 and FUT4 is unclear in trophoblast invasion. The current study aimed to investigate the role of AP1 in uPA/uPAR-induced FUT4 expression and trophoblast invasion...
December 26, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29228607/clinical-significance-and-biological-function-of-fucosyltransferase-2-in-lung-adenocarcinoma
#8
Wenyuan Zhou, Huijun Ma, Guoqing Deng, Lili Tang, Jianxin Lu, Xiaoming Chen
Fucosylation, which is catalyzed by fucosyltransferases (FUTs), is one of the most important glycosylation events involved in cancer. Studies have shown that fucosyltransferase 8 (FUT8) is overexpressed in NSCLC and promotes lung cancer progression. However, there are no reports about the pathological role of fucosyltransferase 2 (FUT2) in lung cancer. To identify FUT2 associated with lung cancer, the expression and clinical significance of FUT2 in lung cancer was investigated by Real-Time PCR, Immunohistochemistry and Western Blot...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29215790/inhibition-of-fucosylation-in-human-invasive-ductal-carcinoma-reduces-e-selectin-ligand-expression-cell-proliferation-and-erk1-2-and-p38-mapk-activation
#9
M A Carrascal, M Silva, J S Ramalho, C Pen, M Martins, C Pascoal, C Amaral, I Serrano, M J Oliveira, R Sackstein, P A Videira
Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases in relation to increased disease progression and metastasis. These glycans in tumor circulating cells mediate binding to vascular E-selectin, initiating tumor extravasation. However, their role(s) in breast carcinogenesis is still unknown. Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling and proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer...
December 7, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/29209018/novel-mechanism-of-the-pericyte-myofibroblast-transition-in-renal-interstitial-fibrosis-core-fucosylation-regulation
#10
Nan Wang, Yiyao Deng, Anqi Liu, Nan Shen, Weidong Wang, Xiangning Du, Qingzhu Tang, Shuangxin Li, Zach Odeh, Taihua Wu, Hongli Lin
Pericytes have been identified as a major source of myofibroblasts in renal interstitial fibrosis (RIF). The overactivation of several signaling pathways, mainly the TGF-β and PDGF pathways, initiates the pericyte-myofibroblast transition during RIF. Key receptors in these two pathways have been shown to be modified by fucosyltransferase 8 (FUT8), the enzyme that catalyzes core fucosylation. This study postulated that core fucosylation might play an important role in regulating the pericyte transition in RIF...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29208566/mir-26a-and-mir-26b-mediate-osteoarthritis-progression-by-targeting-fut4-via-nf-%C3%AE%C2%BAb-signaling-pathway
#11
Jialei Hu, Zi Wang, Yue Pan, Jia Ma, Xiaoyan Miao, Xia Qi, Huimin Zhou, Li Jia
Osteoarthritis (OA) is the most common joint disease, characterized by articular cartilage degradation and changes in all other joint tissues. MicroRNAs (miRNAs) play an important role in mediating the main risk factors for OA. This study aimed to investigate the effect of miR-26a/26b on the proliferation and apoptosis of human chondrocytes by targeting fucosyltransferase 4 (FUT4) through NF-κB signaling pathway. We revealed the differential expression profiles of FUT4 and miR-26a/26b in the articular cartilage tissues of OA patients and normal people...
December 2, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29186206/genetic-signatures-for-helicobacter-pylori-strains-of-west-african-origin
#12
Kennady K Bullock, Carrie L Shaffer, Andrew W Brooks, Ousman Secka, Mark H Forsyth, Mark S McClain, Timothy L Cover
Helicobacter pylori is a genetically diverse bacterial species that colonizes the stomach in about half of the human population. Most persons colonized by H. pylori remain asymptomatic, but the presence of this organism is a risk factor for gastric cancer. Multiple populations and subpopulations of H. pylori with distinct geographic distributions are recognized. Genetic differences among these populations might be a factor underlying geographic variation in gastric cancer incidence. Relatively little is known about the genomic features of African H...
2017: PloS One
https://www.readbyqxmd.com/read/29176671/inhibition-of-delta-induced-notch-signaling-using-fucose-analogs
#13
Michael Schneider, Vivek Kumar, Lars Ulrik Nordstrøm, Lei Feng, Hideyuki Takeuchi, Huilin Hao, Vincent C Luca, K Christopher Garcia, Pamela Stanley, Peng Wu, Robert S Haltiwanger
Notch is a cell-surface receptor that controls cell-fate decisions and is regulated by O-glycans attached to epidermal growth factor-like (EGF) repeats in its extracellular domain. Protein O-fucosyltransferase 1 (Pofut1) modifies EGF repeats with O-fucose and is essential for Notch signaling. Constitutive activation of Notch signaling has been associated with a variety of human malignancies. Therefore, tools that inhibit Notch activity are being developed as cancer therapeutics. To this end, we screened L-fucose analogs for their effects on Notch signaling...
November 27, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/29138114/the-%C3%AE-1-3-fucosyltransferase-fut7-regulates-il-1%C3%AE-induced-monocyte-endothelial-adhesion-via-fucosylation-of-endomucin
#14
Jun Zhang, Nana Ju, Xi Yang, Linmu Chen, Chao Yu
Monocyte-endothelial adhesion is a hallmark feature of atherosclerosis at early stage and emerging evidence suggests that the glycosylation of vascular adhesive molecules and its ligands is involved in this process. Nevertheless, the mechanism underlying this process remains incompletely elucidated. In this study, we reported that treatment with inflammatory factors interleukin-1β (IL-1β) pronouncedly upregulated α1,3-fucosyltransferase VII gene (FUT7) mRNA and protein expression level in EA.hy926 endothelial cells...
November 11, 2017: Life Sciences
https://www.readbyqxmd.com/read/29130097/c-fos-mediates-%C3%AE-1-2-fucosyltransferase-1-and-lewis%C3%A2-y-expression-in-response-to-tgf-%C3%AE-1-in-ovarian-cancer
#15
Yingying Hao, Liancheng Zhu, Limei Yan, Juanjuan Liu, Dawo Liu, Na Gao, Mingzi Tan, Song Gao, Bei Lin
FUT1 is a key rate-limiting enzyme in the synthesis of Lewis y, a membrane-associated carbohydrate antigen. The aberrant upregulation of FUT1 and Lewis y antigen is related to proliferation, invasion and prognosis in malignant epithelial tumors. A c-Fos/activator protein-1 (AP-1) binding site was found in the FUT1 promoter. However, the mechanisms of transcriptional regulation of FUT1 remain poorly understood. TGF-β1 is positively correlated to Lewis y. In the present study, we investigated the molecular mechanism of FUT1 gene expression in response to TGF-β1...
December 2017: Oncology Reports
https://www.readbyqxmd.com/read/29104485/cell-recognition-molecule-l1-regulates-cell-surface-glycosylation-to-modulate-cell-survival-and-migration
#16
Gang Shi, Yue Du, Yali Li, Yue An, Zhenwei He, Yingwei Lin, Rui Zhang, Xiaofei Yan, Jianfeng Zhao, Shihua Yang, Pang Nghee Kheem Brendan, Fang Liu
Background: Cell recognition molecule L1 (L1) plays an important role in cancer cell differentiation, proliferation, migration and survival, but its mechanism remains unclear. Methodology/Principal: Our previous study has demonstrated that L1 enhanced cell survival and migration in neural cells by regulating cell surface glycosylation. In the present study, we show that L1 affected cell migration and survival in CHO (Chinese hamster ovary) cell line by modulation of sialylation and fucosylation at the cell surface via the PI3K (phosphoinositide 3-kinase) and Erk (extracellularsignal-regulated kinase) signaling pathways...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29095923/pofut1-point-mutations-that-disrupt-o-fucosyltransferase-activity-destabilize-the-protein-and-abolish-notch1-signaling-during-mouse-somitogenesis
#17
Rieko Ajima, Emiko Suzuki, Yumiko Saga
The segmental pattern of the vertebrate body is established via the periodic formation of somites from the presomitic mesoderm (PSM). This periodical process is controlled by the cyclic and synchronized activation of Notch signaling in the PSM. Protein O-fucosyltransferase1 (Pofut1), which transfers O-fucose to the EGF domains of the Notch1 receptor, is indispensable for Notch signaling activation. The Drosophila homologue Ofut1 was reported to control Notch localization via two different mechanisms, working as a chaperone for Notch or as a regulator of Notch endocytosis...
2017: PloS One
https://www.readbyqxmd.com/read/29079498/infection-s-sweet-tooth-how-glycans-mediate-infection-and-disease-susceptibility
#18
REVIEW
Steven L Taylor, Michael A McGuckin, Steve Wesselingh, Geraint B Rogers
Glycans form a highly variable constituent of our mucosal surfaces and profoundly affect our susceptibility to infection and disease. The diversity and importance of these surface glycans can be seen in individuals who lack a functional copy of the fucosyltransferase gene, FUT2. Representing around one-fifth of the population, these individuals have an altered susceptibility to many bacterial and viral infections and diseases. The mediation of host-pathogen interactions by mucosal glycans, such as those added by FUT2, is poorly understood...
October 24, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28990779/designer-%C3%AE-1-6-fucosidase-mutants-enable-direct-core-fucosylation-of-intact-n-glycopeptides-and-n-glycoproteins
#19
Chao Li, Shilei Zhu, Christopher Ma, Lai-Xi Wang
Core fucosylation of N-glycoproteins plays a crucial role in modulating the biological functions of glycoproteins. Yet, the synthesis of structurally well-defined, core-fucosylated glycoproteins remains a challenging task due to the complexity in multistep chemical synthesis or the inability of the biosynthetic α1,6-fucosyltransferase (FUT8) to directly fucosylate full-size mature N-glycans in a chemoenzymatic approach. We report in this paper the design and generation of potential α1,6-fucosynthase and fucoligase for direct core fucosylation of intact N-glycoproteins...
October 25, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28988527/how-fucose-of-blood-group-glycotopes-programs-human-gut-microbiota
#20
REVIEW
S V Kononova
Formation of appropriate gut microbiota is essential for human health. The first two years of life is the critical period for this process. Selection of mutualistic microorganisms of the intestinal microbiota is controlled by the FUT2 and FUT3 genes that encode fucosyltransferases, enzymes responsible for the synthesis of fucosylated glycan structures of mucins and milk oligosaccharides. In this review, the mechanisms of the selection and maintenance of intestinal microorganisms that involve fucosylated oligosaccharides of breast milk and mucins of the newborn's intestine are described...
September 2017: Biochemistry. Biokhimii︠a︡
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