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https://www.readbyqxmd.com/read/27888602/a-glycovariant-of-human-cd44-is-characteristically-expressed-on-human-mesenchymal-stem-cells
#1
Gisela Pachón-Peña, Conor Donnelly, Catalina Ruiz-Cañada, Adam Katz, Sonia Fernández-Veledo, Joan Vendrell, Robert Sackstein
The clinical effectiveness of systemically-administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase- VI (FTVI) to enforce sLe(X) decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin...
November 26, 2016: Stem Cells
https://www.readbyqxmd.com/read/27871849/cd44-fucosylation-on-mesenchymal-stem-cell-enhances-homing-and-macrophage-polarization-in-ischemic-kidney-injury
#2
Kang-Ju Chou, Po-Tsang Lee, Chien-Liang Chen, Chih-Yang Hsu, Wei-Chieh Huang, Chien-Wei Huang, Hua-Chang Fang
The lack of homing ability possibly reduces the healing potential of bone-marrow-derived mesenchymal stem cells (MSCs). Therefore, transforming native CD44 on MSCs into a hematopoietic cell E-/L-selectin ligand (HCELL) that possesses potent E-selectin affinity might enhance the homing and regenerative abilities of MSCs. Through fucosyltransferase VI (FTVI) transfection, MSCs were fucosylated on N-glycans of CD44 to become HCELL positive, thus interacting with E-selectin on injured endothelial cells. HCELL expression facilitated MSC homing in kidneys within 24hours after injury and reduced lung stasis...
November 18, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27870886/comparative-genomics-revealed-multiple-helicobacter-pylori-genes-associated-with-biofilm-formation-in-vitro
#3
Eric Hong Jian Wong, Chow Goon Ng, Eng Guan Chua, Alfred Chin Yen Tay, Fanny Peters, Barry J Marshall, Bow Ho, Khean Lee Goh, Jamuna Vadivelu, Mun Fai Loke
BACKGROUND: Biofilm formation by Helicobacter pylori may be one of the factors influencing eradication outcome. However, genetic differences between good and poor biofilm forming strains have not been studied. MATERIALS AND METHODS: Biofilm yield of 32 Helicobacter pylori strains (standard strain and 31 clinical strains) were determined by crystal-violet assay and grouped into poor, moderate and good biofilm forming groups. Whole genome sequencing of these 32 clinical strains was performed on the Illumina MiSeq platform...
2016: PloS One
https://www.readbyqxmd.com/read/27858203/enhanced-fucosylation-of-ga1-in-the-digestive-tracts-of-x-ray-irradiated-mice
#4
Masao Iwamori, Kyoko Tanaka, Shigeki Adachi, Daisuke Aoki, Taisei Nomura
In mice at 4 days after X-ray-irradiation at 0.5 Gy/min for 16 min, the tissue weights of immune organs, i.e., thymus and spleen, were decreased due to injury to lymphocytes by the X-rays. The resulting immunosuppressive condition allowed the growth of lactobacilli, i.e., L. murinus, which contained LacβTH-DG and possessed the ability to induce transcription of the fucosyltransferase gene for synthesis of FGA1. LacβTH-DG was detected in the jejunal and ileal contents of X-ray-irradiated mice, but not in those of control mice, whereas LacTetH-DG of L...
November 18, 2016: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27798070/circulating-blood-and-platelets-supply-glycosyltransferases-that-enable-extrinsic-extracellular-glycosylation
#5
Melissa M Lee-Sundlov, David J Ashline, Andrew J Hanneman, Renata Grozovsky, Vernon N Reinhold, Karin M Hoffmeister, Joseph Ty Lau
Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on distal cell surfaces and in the extracellular milieu, the glycan-modifying activities present in systemic circulation have not been systematically examined. Here, we describe an evaluation of blood-borne sialyl-, galactosyl- and fucosyltransferase activities that act upon the four common terminal glycan precursor motifs, GlcNAc monomer, Gal(β3)GlcNAc, Gal(β4)GlcNAc and Gal(β3)GalNAc, to produce more complex glycan structures...
October 26, 2016: Glycobiology
https://www.readbyqxmd.com/read/27773703/n-glycoform-dependent-interactions-of-megalin-with-its-ligands
#6
Makoto Hirano, Kiichiro Totani, Tomohiko Fukuda, Jianguo Gu, Akemi Suzuki
BACKGROUND: Megalin is a 600-kDa single-spanning transmembrane glycoprotein and functions as an endocytic receptor, distributed not only in the kidney but also in other tissues. Structurally and functionally distinct ligands for megalin have been identified. Megalin has 30 potential N-glycosylation sites in its extracellular domain. We found that megalin interacts with its ligands in a glycoform-dependent manner. METHODS: Distribution of megalin and glycans was histochemically analyzed in mouse kidneys...
October 20, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27773385/altered-fucosyltransferase-expression-in-the-superior-temporal-gyrus-of-elderly-patients-with-schizophrenia
#7
Toni M Mueller, Stefani D Yates, Vahram Haroutunian, James H Meador-Woodruff
Glycosylation is a post-translational modification that is an essential element in cell signaling and neurodevelopmental pathway regulation. Glycan attachment can influence the tertiary structure and molecular interactions of glycosylated substrates, adding an additional layer of regulatory complexity to functional mechanisms underlying central cell biological processes. One type of enzyme-mediated glycan attachment, fucosylation, can mediate glycoprotein and glycolipid cell surface expression, trafficking, secretion, and quality control to modulate a variety of inter- and intracellular signaling cascades...
October 20, 2016: Schizophrenia Research
https://www.readbyqxmd.com/read/27760104/epithelial-glycosylation-in-gut-homeostasis-and-inflammation
#8
Yoshiyuki Goto, Satoshi Uematsu, Hiroshi Kiyono
Intestinal epithelial cells apically express glycans, especially α1,2-fucosyl linkages, which work as a biological interface for the host-microbe interaction. Emerging studies have shown that epithelial α1,2-fucosylation is regulated by microbes and by group 3 innate lymphoid cells (ILC3s). Dysregulation of the gene (FUT2) encoding fucosyltransferase 2, an enzyme governing epithelial α1,2-fucosylation, is associated with various human disorders, including infection and chronic inflammatory diseases. This suggests a critical role for an interaction between microbes, epithelial cells and ILC3s mediated via glycan residues...
October 19, 2016: Nature Immunology
https://www.readbyqxmd.com/read/27757838/fucosyltransferase-4-and-oligosaccharide-lewis-y-antigen-as-potentially-correlative-biomarkers-of-helicobacter-pylori-caga-associated-gastric-cancer
#9
Faisal Aziz, Wei Gao, Qiu Yan
H. pylori cytotoxin associated antigen A (CagA) plays a significant role in the progression of gastric cancer but their effect on fucosylation to develop gastric cancer is unknown. Fucosyltransferase IV (FUT4) is the key enzyme for synthesis of LewisY (LeY) carried by glycoproteins and glycolipids on the cell membrane. Herein, we compare the expression of CagA, p-EGFR, FUT4 and LeY in gastritis (n = 128, 176), gastric ulcer (n = 174, 213), and gastric cancer (n = 323, 261) tissue and serum samples, respectively by IHC and ELISA...
October 18, 2016: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/27687499/impaired-adamts9-secretion-a-potential-mechanism-for-eye-defects-in-peters-plus-syndrome
#10
Johanne Dubail, Deepika Vasudevan, Lauren W Wang, Sarah E Earp, Michael W Jenkins, Robert S Haltiwanger, Suneel S Apte
Peters Plus syndrome (PPS), a congenital disorder of glycosylation, results from recessive mutations affecting the glucosyltransferase B3GLCT, leading to congenital corneal opacity and diverse extra-ocular manifestations. Together with the fucosyltransferase POFUT2, B3GLCT adds Glucoseβ1-3Fucose disaccharide to a consensus sequence in thrombospondin type 1 repeats (TSRs) of several proteins. Which of these target proteins is functionally compromised in PPS is unknown. We report here that haploinsufficiency of murine Adamts9, encoding a secreted metalloproteinase with 15 TSRs, leads to congenital corneal opacity and Peters anomaly (persistent lens-cornea adhesion), which is a hallmark of PPS...
September 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27681169/another-step-closer-to-understanding-plant-cell-wall-biosynthesis-the-crystal-structure-of-fucosyltransferase-1
#11
Nancy Rosenbaum Hofmann
No abstract text is available yet for this article.
September 28, 2016: Plant Cell
https://www.readbyqxmd.com/read/27637560/structure-of-arabidopsis-thaliana-fut1-reveals-a-variant-of-the-gt-b-class-fold-and-provides-insight-into-xyloglucan-fucosylation
#12
Joana Rocha, Félix Cicéron, Daniele de Sanctis, Mickael Lelimousin, Valérie Chazalet, Olivier Lerouxel, Christelle Breton
The plant cell wall is a complex and dynamic network made mostly of cellulose, hemicelluloses and pectins. Xyloglucan, the major hemicellulosic component in Arabidopsis thaliana, is biosynthesized in the Golgi apparatus by a series of glycan synthases and glycosyltransferases before export to the wall. A better understanding of the xyloglucan biosynthetic machinery will give clues toward engineering plants with improved wall properties or designing novel xyloglucan-based biomaterials. The xyloglucan-specific α-2-fucosyltransferase FUT1 catalyzes the transfer of fucose from GDP-fucose to terminal galactosyl residues on xyloglucan side chains...
September 16, 2016: Plant Cell
https://www.readbyqxmd.com/read/27628655/relationship-between-increased-fucosylation-and-metastatic-potential-in-colorectal-cancer
#13
Takahiro Osuga, Rishu Takimoto, Michihiro Ono, Masahiro Hirakawa, Makoto Yoshida, Yutaka Okagawa, Naoki Uemura, Yohei Arihara, Yasushi Sato, Fumito Tamura, Tsutomu Sato, Satoshi Iyama, Koji Miyanishi, Kohichi Takada, Tsuyoshi Hayashi, Masayoshi Kobune, Junji Kato
BACKGROUND: Fucose is utilized for the modification of different molecules involved in blood group determination, immunological reactions, and signal transduction pathways. We have recently reported that enhanced activity of the fucosyltransferase 3 and/or 6 promoted TGF-ß-mediated epithelial mesenchymal transition and was associated with increased metastatic potential of colorectal cancer (CRC), suggesting that fucose is required by CRC cells. With this in mind, we examined requirement of L-fucose in CRC cells and developed fucose-bound nanoparticles as vehicles for delivery of anticancer drugs specific to CRC...
September 2016: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/27591321/imprinting-of-skin-inflammation-homing-in-cd4-t-cells-is-controlled-by-dna-methylation-within-the-fucosyltransferase-7-gene
#14
Matthias Pink, Boris A Ratsch, Maibritt Mardahl, Pawel Durek, Julia K Polansky, Martin Karl, Ria Baumgrass, Stefan Wallner, Cristina Cadenas, Kathrin Gianmoena, Stefan Floess, Wei Chen, Karl Nordstroem, Sascha Tierling, Sven Olek, Jörn Walter, Alf Hamann, Uta Syrbe
E- and P-selectin ligands (E- and P-ligs) guide effector memory T cells into skin and inflamed regions, mediate the inflammatory recruitment of leukocytes, and contribute to the localization of hematopoietic precursor cells. A better understanding of their molecular regulation is therefore of significant interest with regard to therapeutic approaches targeting these pathways. In this study, we examined the transcriptional regulation of fucosyltransferase 7 (FUT7), an enzyme crucial for generation of the glycosylated E- and P-ligs...
September 2, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27580247/expression-purification-and-biochemical-characterization-of-atfut1-a-xyloglucan-specific-fucosyltransferase-from-arabidopsis-thaliana
#15
Félix Cicéron, Joana Rocha, Sumaira Kousar, Sara Fasmer Hansen, Valérie Chazalet, Emilie Gillon, Christelle Breton, Olivier Lerouxel
Efforts to identify genes and characterize enzymes involved in the biosynthesis of plant cell wall polysaccharides have yet to produce and purify to homogeneity an active plant cell wall synthesizing enzyme suitable for structural studies. In Arabidopsis, the last step of xyloglucan (XG) biosynthesis is catalyzed by fucosyltransferase 1 (AtFUT1), which transfers l-fucose from GDP-β-l-fucose to a specific galactose on the XG core. Here, we describe the production of a soluble form of AtFUT1 (HisΔ68-AtFUT1) and its purification to milligram quantities...
September 2016: Biochimie
https://www.readbyqxmd.com/read/27560716/fucosylation-of-lamp-1-and-lamp-2-by-fut1-correlates-with-lysosomal-positioning-and-autophagic-flux-of-breast-cancer-cells
#16
Keng-Poo Tan, Ming-Yi Ho, Huan-Chieh Cho, John Yu, Jung-Tung Hung, Alice Lin-Tsing Yu
Alpha1,2-fucosyltransferases, FUT1 and FUT2, which transfer fucoses onto the terminal galactose of N-acetyl-lactosamine via α1,2-linkage have been shown to be highly expressed in various types of cancers. A few studies have shown the involvement of FUT1 substrates in tumor cell proliferation and migration. Lysosome-associated membrane protein 1, LAMP-1, has been reported to carry alpha1,2-fucosylated Lewis Y (LeY) antigens in breast cancer cells, however, the biological functions of LeY on LAMP-1 remain largely unknown...
August 25, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27550195/introduction-of-h-antigens-into-oligosaccharides-and-sugar-chains-of-glycoproteins-using-highly-efficient-1-2-%C3%AE-l-fucosynthase
#17
Yuta Sugiyama, Aina Gotoh, Toshihiko Katoh, Yuji Honda, Erina Yoshida, Shin Kurihara, Hisashi Ashida, Hidehiko Kumagai, Kenji Yamamoto, Motomitsu Kitaoka, Takane Katayama
Fucα1-2 Gal linkages, or H-antigens, constitute histo-blood group antigens and are involved in various physiological processes. In addition, recent studies have shown that the H-antigen-containing glycans play an important role, not only in establishing harmonious relationship between gut microbes and the host, but also in preventing gut dysbiosis-related diseases. Therefore, development of an efficient method for introducing Fuc residue at Gal residue at the non-reducing end of glycans via α-(1→2) linkage is desired for research as well as medicinal purposes...
August 22, 2016: Glycobiology
https://www.readbyqxmd.com/read/27533464/comprehensive-n-glycan-profiles-of-hepatocellular-carcinoma-reveal-association-of-fucosylation-with-tumor-progression-and-regulation-of-fut8-by-micrornas
#18
Lei Cheng, Shuhang Gao, Xiaobo Song, Weijie Dong, Huimin Zhou, Lifen Zhao, Li Jia
Glycosylation has significant effects on cancer progression. Fucosylation is one of the most important glycosylation events involved in hepatocellular carcinoma (HCC). Here, we compared N-glycan profiles of liver tumor tissues and adjacent tissues of 27 HCC patients to reveal the association between fucosylation and HCC progression, as well as verified the potential role of miRNA in regulating fucosylation. Mass spectrometry (MS) analysis showed pronounced differences of the N-glycosylation patterns and fucosylated N-glycans between the adjacent and tumor tissues...
August 13, 2016: Oncotarget
https://www.readbyqxmd.com/read/27521658/inhibiting-post-translational-core-fucosylation-prevents-vascular-calcification-in-the-model-of-uremia
#19
Xinyu Wen, Anqi Liu, Changqing Yu, Lingyu Wang, Mengying Zhou, Nan Wang, Ming Fang, Weidong Wang, Hongli Lin
Vascular calcification (VC) is an independent risk factor for cardiovascular disease and mortality in uremia. Post-translational core fucosylation is implicated in a number of pathological processes. First, we investigated the role of core fucosylation and key TGF-β1 pathway receptors in calcified arteries in vivo. To determine whether blocking core fucosylation effectively inhibited VC and TGF-β/Smad signaling pathway, we established an in vitro model of phosphate-induced calcification in rat vascular smooth muscle cells (VSMCs) to assess the role of core fucosylation in VC...
October 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27519168/microrna-106b-targets-fut6-to-promote-cell-migration-invasion-and-proliferation-in-human-breast-cancer
#20
Nana Li, Yuejian Liu, Yuan Miao, Lifen Zhao, Huimin Zhou, Li Jia
It is demonstrated that the maladjustment of microRNA (miRNA) plays significant roles in the occurrence and development of tumors. MicroRNA-106b-5p (miR-106b), a carcinogenic miRNA, is identified as a dysregulated miRNA in human breast cancer. In this article, the expression levels of miR-106b were discovered to be particularly higher in breast cancer tissues than that in the corresponding adjacent tissues. Accordingly, miR-106b was higher expressed in the breast cancer cell lines compared with that in the normal breast cell lines...
September 2016: IUBMB Life
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