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https://www.readbyqxmd.com/read/29234464/production-of-isoprene-one-of-the-high-density-fuel-precursors-from-peanut-hull-using-the-high-efficient-lignin-removal-pretreatment-method
#1
Sumeng Wang, Zhaobao Wang, Yongchao Wang, Qingjuan Nie, Xiaohua Yi, Wei Ge, Jianming Yang, Mo Xian
Background: Isoprene as the feedstock can be used to produce renewable energy fuels, providing an alternative to replace the rapidly depleting fossil fuels. However, traditional method for isoprene production could not meet the demands for low-energy consumption and environment-friendliness. Moreover, most of the previous studies focused on biofuel production out of lignocellulosic materials such as wood, rice straw, corn cob, while few studies concentrated on biofuel production using peanut hull (PH)...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29223503/multiple-collections-of-common-carp-cyprinus-carpio-l-semen-during-the-reproductive-period-and-its-effects-on-sperm-quality
#2
Beata Irena Cejko, Beata Sarosiek, Sławomir Krejszeff, Radosław Kajetan Kowalski
Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L. were used to analyse changes in semen quality. Semen collection was performed on June 1 (first collection), 12 (second collection), and 19 (third collection) from individual males (n=11) by gentle abdominal massage. Semen quantity (semen volume and sperm count), quality (sperm motility and sperm viability), as well as seminal plasma parameters (pH of seminal plasma and seminal plasma osmotic pressure) and its enzymatic activity, e...
December 5, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29183414/effects-of-soluble-fiber-inclusion-in-gestation-diets-with-varying-fermentation-characteristics-on-lactational-feed-intake-of-sows-over-two-successive-parities
#3
C Q Tan, H Q Sun, H K Wei, J J Tan, G Long, S W Jiang, J Peng
The effects of soluble fiber inclusion in gestation diets with varying fermentation characteristics (fermentation kinetics and short-chain fatty acids (SCFA)-profile) on lactational feed intake of sows and their piglet growth over two parities were investigated using an in vitro-in vivo methodology. After breeding, 90 multiparous Landrace sows were randomized to one of three experimental diets: the control (CON) diet, konjac flour (KF) diet or sugar beet pulp (SBP) diet. All diets had similar levels of net energy, CP, insoluble fiber and NDF, but KF and SBP diets had higher soluble fiber levels than the CON diet...
November 29, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29182315/determinants-and-prediction-of-esterase-substrate-promiscuity-patterns
#4
Monica Martínez-Martínez, Cristina Coscolín, Gerard Santiago, Jennifer Chow, Peter J Stogios, Rafael Bargiela, Christoph Gertler, José Navarro-Fernández, Alexander Bollinger, Stephan Thies, Celia Méndez-García, Ana Popovic, Greg Brown, Tatyana N Chernikova, Antonio García-Moyano, Gro Ek Bjergah, Pablo Pérez-García, Tran Hai, Mercedes V Del Pozo, Runar Stokke, Ida H Steen, Hong Cui, Xiaohui Xu, Boguslaw P Nocek, María Alcaide, Marco Distaso, Victoria Mesa, Ana I Pelaez, Jesús Sánchez, Patrick C F Buchholz, Juergen Pleiss, Antonio Fernández-Guerra, Frank O Glöckner, Olga V Golyshina, Michail M Yakimov, Alexei Savchenko, Karl-Erich Jaeger, Alexander F Yakunin, Wolfgang R Streit, Peter N Golyshin, Victor Guallar, Manuel Ferrer
Esterases receive special attention because their wide distribution in biological systems and environments and their importance for physiology and chemical synthesis. The prediction of esterases substrate promiscuity level from sequence data and the molecular reasons why certain such enzymes are more promiscuous than others, remain to be elucidated. This limits the surveillance of the sequence space for esterases potentially leading to new versatile biocatalysts and new insights into their role in cellular function...
November 28, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/29175057/simvastatin-blocks-soluble-ssao-vap-1-release-in-experimental-models-of-cerebral-ischemia-possible-benefits-for-stroke-induced-inflammation-control
#5
Ping Sun, Mar Hernandez-Guillamón, Mireia Campos-Martorell, Alba Simats, Joan Montaner, Mercedes Unzeta, Montse Solé
Beyond cholesterol reduction, statins mediate their beneficial effects on stroke patients through pleiotropic actions. They have shown anti-inflammatory properties by a number of different mechanisms, including the inhibition of NF-κB transcriptional activity and the consequent increase and release of adhesion molecules. We have studied simvastatin's effects on the vascular enzyme semicarbazide-sensitive amine oxidase/vascular adhesion protein 1 (SSAO/VAP-1), which is involved in stroke-mediated brain injury...
November 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29153568/maternal-obesity-and-cardiac-development-in-the-offspring-study-in-human-neonates-and-minipigs
#6
Maria Angela Guzzardi, Tiziana Liistro, Luna Gargani, Lamia Ait Ali, Gennaro D'Angelo, Silvia Rocchiccioli, Federica La Rosa, Alessandra Kemeny, Elena Sanguinetti, Nadia Ucciferri, Mariarosaria De Simone, Antonietta Bartoli, Pierluigi Festa, Piero A Salvadori, Silvia Burchielli, Rosa Sicari, Patricia Iozzo
OBJECTIVES: The aim of this study was to investigate the consequences of maternal overweight on cardiac development in offspring in infants (short term) and minipigs (short and longer term). BACKGROUND: The epidemic of overweight involves pregnant women. The uterine environment affects organ development, modulating disease susceptibility. Offspring of obese mothers have higher rates of cardiovascular events and mortality. METHODS: Echocardiography was performed in infants born to lean and overweight mothers at birth and at 3, 6, and 12 months of age...
November 10, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/29150708/production-of-arabitol-from-enzymatic-hydrolysate-of-soybean-flour-by-debaryomyces-hansenii-fermentation
#7
Abdullah A Loman, S M M Islam, Lu-Kwang Ju
Arabitol is a low-calorie sugar alcohol with anti-cariogenic properties. Enzymatic hydrolysate of soybean flour is a new renewable biorefinery feedstock containing hexose, pentose, and organic nitrogen sources. Arabitol production by Debaryomyces hansenii using soybean flour hydrolysate was investigated. Effects of medium composition, operating conditions, and culture stage (growing or stationary phase) were studied. Production was also compared at different culture volumes to understand the effect of dissolved oxygen concentration (DO)...
November 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29121900/synergistic-gastroprotective-activity-of-methanolic-extract-of-a-mixture-of-melastoma-malabathricum-and-muntingia-calabura-leaves-in-rats
#8
Siti Zawanah Halim, Zainul Amiruddin Zakaria, Maizatul Hasyima Omar, Norhafizah Mohtarrudin, Ikarastika Rahayu Abdul Wahab, Muhammad Nazrul Hakim Abdullah
BACKGROUND: Melastoma malabathricum L. (family Melastomaceae; MM) and Muntingia calabura L. (family Elaeocarpaceae; MC) have been separately reported to possess gastroprotective activity. In an attempt to develop a pharmaceutical product with antiulcer potential, the synergistic gastroprotective activity of methanolic extract of a mixture of MM and MC (MMMC) at various ratios was evaluated in rat models. METHODS: Rats were pre-treated orally with 2% Tween 80 (vehicle), 100 mg/kg ranitidine (reference drug) or MMMC (ratios of 1:1, 1:3 and 3:1 (v/v); doses of 15, 150 or 300 mg/kg) and then subjected to the ethanol-induced gastric ulcer or pyloric ligation assays...
November 9, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29112289/pharmacokinetics-of-intravenous-lithium-chloride-and-assessment-of-agreement-between-two-methods-of-lithium-concentration-measurement-in-the-horse
#9
L M Martin, A D Bukoski, D D Whelchel, T J Evans, C E Wiedmeyer, S J Black, P J Johnson
BACKGROUND: Pharmacokinetics of lithium chloride (LiCl) administered as a bolus, once intravenously (i.v.) have not been determined in horses. There is no point-of-care test to measure lithium (Li(+) ) concentrations in horses in order to monitor therapeutic levels and avoid toxicity. OBJECTIVES: Determine the pharmacokinetics of LiCl in healthy adult horses and compare agreement between two methods of measurement of plasma Li(+) concentration: spectrophotometric enzymatic assay (SEA) and inductively coupled plasma mass spectrometry (ICP-MS)...
November 7, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29032194/in-situ-mucoadhesive-thermosensitive-liposomal-gel-as-a-novel-vehicle-for-nasal-extended-delivery-of-opiorphin
#10
Paola Mura, Natascia Mennini, Cristina Nativi, Barbara Richichi
Previous studies proved the effectiveness of an intravenous PEGylated liposomal formulation of opiorphin (1 mg/mL) in protecting the drug from enzymatic degradation, and improving intensity and duration of its painkilling effect. Therefore, considering the advantages of nasal administration, the aim of this work was the development of a liposomal mucoadhesive thermo-sensitive in situ gel for the extended nasal delivery of opiorphin. With this purpose, the potential of a series of combinations of different polymers (i...
October 12, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29030301/biodegradable-zwitterionic-sulfobetaine-polymer-and-its-conjugate-with-paclitaxel-for-sustained-drug-delivery
#11
Haotian Sun, Michael Yu Zarng Chang, Wei-I Cheng, Qing Wang, Alex Commisso, Meghan Capeling, Yun Wu, Chong Cheng
A fully biodegradable zwitterionic polymer and the corresponding conjugate with paclitaxel (PTX) were synthesized as promising biomaterials. Allyl-functionalized polylactide (PLA) was employed as the precursor of polymer backbones. UV-induced thiol-ene reaction was conducted to conjugate thiol-functionalized sulfobetaine (SB) with the PLA-based backbone. The resulting zwitterionic polymer did not exhibit considerable cytotoxicity. A polymer-drug conjugate was also obtained by thiol-ene reaction of both thiol-functionalized SB and PTX with allyl-functionalized PLA...
December 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28986983/enzymatic-synthesis-of-chiral-amino-alcohols-by-coupling-transketolase-and-transaminase-catalyzed-reactions-in-a-cascading-continuous-flow-microreactor-system
#12
Pia Gruber, Filipe Carvalho, Marco P C Marques, Brian O'Sullivan, Fabiana Subrizi, Dragana Dobrijevic, John Ward, Helen C Hailes, Pedro Fernandes, Roland Wohlgemuth, Frank Baganz, Nicolas Szita
Rapid biocatalytic process development and intensification continues to be challenging with currently available methods. Chiral amino-alcohols are of particular interest as they represent key industrial synthons for the production of complex molecules and optically pure pharmaceuticals. (2S,3R)-2-amino-1,3,4-butanetriol (ABT), a building block for the synthesis of protease inhibitors and detoxifying agents, can be synthesized from simple, non-chiral starting materials, by coupling a transketolase- and a transaminase-catalyzed reaction...
October 7, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28976432/the-efficiency-of-bone-marrow-aspiration-for-the-harvest-of-connective-tissue-progenitors-from-the-human-iliac-crest
#13
Thomas E Patterson, Cynthia Boehm, Chizu Nakamoto, Richard Rozic, Esteban Walker, Nicolas S Piuzzi, George F Muschler
BACKGROUND: The rational design and optimization of tissue engineering strategies for cell-based therapy requires a baseline understanding of the concentration and prevalence of osteogenic progenitor cell populations in the source tissues. The aim of this study was to (1) define the efficiency of, and variation among individuals in, bone marrow aspiration as a means of osteogenic connective tissue progenitor (CTP-O) harvest compared with harvest from iliac cancellous bone, and (2) determine the location of CTP-Os within native cancellous bone and their distribution between the marrow-space and trabecular-surface tissue compartments...
October 4, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28962879/identification-and-characterization-of-a-thermostable-endolytic-%C3%AE-agarase-aga2-from-a-newly-isolated-marine-agarolytic-bacteria-cellulophaga-omnivescoria-w5c
#14
Kristine Rose M Ramos, Kris Niño G Valdehuesa, Grace M Nisola, Won-Keun Lee, Wook-Jin Chung
Research on the enzymatic breakdown of seaweed-derived agar has recently gained attention due to the progress in green technologies for marine biomass utilization. The enzymes known as agarases catalyze the cleavage of glycosidic bonds within the polysaccharide. In this study, a new β-agarase, Aga2, was identified from Cellulophaga omnivescoria W5C. Aga2 is one of four putative agarases from the W5C genome which belong to the glycoside hydrolase 16 family. It was shown to be exclusive to the Cellulophaga genus...
September 26, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28961225/graphene-quantum-dots-electrochemistry-and-sensitive-electrocatalytic-glucose-sensor-development
#15
Sanju Gupta, Tyler Smith, Alexander Banaszak, John Boeckl
Graphene quantum dots (GQDs), derived from functionalized graphene precursors are graphene sheets a few nanometers in the lateral dimension having a several-layer thickness. They are zero-dimensional materials with quantum confinement and edge site effects. Intense research interest in GQDs is attributed to their unique physicochemical phenomena arising from the sp²-bonded carbon nanocore surrounded with edged plane functional moieties. In this work, GQDs are synthesized by both solvothermal and hydrothermal techniques, with the optimal size of 5 nm determined using high-resolution transmission electron microscopy, with additional UV-Vis absorption and fluorescence spectroscopy, revealing electronic band signatures in the blue-violet region...
September 29, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28942312/evaluation-of-benefits-and-risks-associated-with-the-agricultural-use-of-organic-wastes-of-pharmaceutical-origin
#16
Mirko Cucina, Chiara Tacconi, Anna Ricci, Daniela Pezzolla, Simone Sordi, Claudia Zadra, Giovanni Gigliotti
Industrial fermentations for the production of pharmaceuticals generate large volumes of wastewater that can be biologically treated to recover plant nutrients through the application of pharmaceutical-derived wastes to the soil. Nevertheless, benefits and risks associated with their recovery are still unexplored. Thus, the aim of the present work was to characterize three potential organic residues (sludge, anaerobic digestate and compost) derived from the wastewater generated by the daptomycin production process...
February 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/28923765/preparation-and-investigation-of-p28gst-loaded-plga-microparticles-for-immunomodulation-of-experimental-colitis
#17
T H Hoang Thi, P A Priemel, Y Karrout, V Driss, M Delbeke, A Dendooven, M P Flament, M Capron, J Siepmann
The aim of this work was to prepare and characterize (in vitro and in vivo) PLGA-based microparticles loaded with an enzymatic protein derived from the helminth parasite Schistosoma haematobium: glutathione S-transferase P28GST (P28GST). This protein is not only a promising candidate vaccine against schistosomiasis, it also exhibits interesting immunomodulating effects, which can be helpful for the regulation of inflammatory diseases. Helminths express a regulatory role on intestinal inflammation, and immunization by P28GST has recently been shown to be as efficient as infection to reduce inflammation in a murine colitis model...
November 25, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28915417/lc-ms-ms-quantification-of-7%C3%AE-hydroxy-4-cholesten-3-one-c4-in-rat-and-monkey-plasma
#18
Lijuan Kang, Thomas M Connolly, Naidong Weng, Wenying Jian
7α-hydroxy-4-cholesten-3-one (C4) is an oxidative enzymatic product of cholesterol metabolism via cholesterol 7α-hydroxylase, an enzyme also known as cholesterol 7-alpha-monooxygenase or cytochrome P450 7A1 (CYP7A1). C4 is a stable intermediate in the rate limiting pathway of bile acid biosynthesis. Previous studies showed that plasma C4 levels correlated with CYP7A1 enzymatic activity and could serve as a biomarker for bile acid synthesis. Here we developed and qualified a simple and robust high-throughput method using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to quantify C4 in rat and monkey plasma...
October 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28914766/development-of-a-sensitive-electrochemical-enzymatic-reaction-based-cholesterol-biosensor-using-nano-sized-carbon-interdigitated-electrodes-decorated-with-gold-nanoparticles
#19
Deepti Sharma, Jongmin Lee, Junyoung Seo, Heungjoo Shin
We developed a versatile and highly sensitive biosensor platform. The platform is based on electrochemical-enzymatic redox cycling induced by selective enzyme immobilization on nano-sized carbon interdigitated electrodes (IDEs) decorated with gold nanoparticles (AuNPs). Without resorting to sophisticated nanofabrication technologies, we used batch wafer-level carbon microelectromechanical systems (C-MEMS) processes to fabricate 3D carbon IDEs reproducibly, simply, and cost effectively. In addition, AuNPs were selectively electrodeposited on specific carbon nanoelectrodes; the high surface-to-volume ratio and fast electron transfer ability of AuNPs enhanced the electrochemical signal across these carbon IDEs...
September 15, 2017: Sensors
https://www.readbyqxmd.com/read/28900508/direct-macromolecular-drug-delivery-to-cerebral-ischemia-area-using-neutrophil-mediated-nanoparticles
#20
Chun Zhang, Cheng-Li Ling, Liang Pang, Qi Wang, Jing-Xin Liu, Bing-Shan Wang, Jian-Ming Liang, Yi-Zhen Guo, Jing Qin, Jian-Xin Wang
Delivery of macromolecular drugs to the brain is impeded by the blood brain barrier. The recruitment of leukocytes to lesions in the brain, a typical feature of neuroinflammation response which occurs in cerebral ischemia, offers a unique opportunity to deliver drugs to inflammation sites in the brain. In the present study, cross-linked dendrigraft poly-L-lysine (DGL) nanoparticles containing cis-aconitic anhydride-modified catalase and modified with PGP, an endogenous tripeptide that acts as a ligand with high affinity to neutrophils, were developed to form the cl PGP-PEG-DGL/CAT-Aco system...
2017: Theranostics
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