keyword
MENU ▼
Read by QxMD icon Read
search

Hif 2

keyword
https://www.readbyqxmd.com/read/29333666/stable-high-index-faceted-pt-skin-on-zigzag-like-ptfe-nanowires-enhances-oxygen-reduction-catalysis
#1
Mingchuan Luo, Yingjun Sun, Xu Zhang, Yingnan Qin, Mingqiang Li, Yingjie Li, Chunji Li, Yong Yang, Lei Wang, Peng Gao, Gang Lu, Shaojun Guo
Selectively exposing active surfaces and judiciously tuning the near-surface composition of electrode materials represent two prominent means of promoting electrocatalytic performance. Here, a new class of Pt3 Fe zigzag-like nanowires (Pt-skin Pt3 Fe z-NWs) with stable high-index facets (HIFs) and nanosegregated Pt-skin structure is reported, which are capable of substantially boosting electrocatalysis in fuel cells. These unique structural features endow the Pt-skin Pt3 Fe z-NWs with a mass activity of 2.11 A mg-1 and a specifc activity of 4...
January 15, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29333385/kaempferol-inhibits-angiogenesis-by-suppressing-hif-1%C3%AE-and-vegfr2-activation-via-erk-p38-mapk-and-pi3k-akt-mtor-signaling-pathways-in-endothelial-cells
#2
Gi Dae Kim
Kaempferol has been shown to inhibit vascular formation in endothelial cells. However, the underlying mechanisms are not fully understood. In the present study, we evaluated whether kaempferol exerts antiangiogenic effects by targeting extracellular signal-regulated kinase (ERK)/p38 mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt/mechanistic target of rapamycin (mTOR) signaling pathways in endothelial cells. Endothelial cells were treated with various concentrations of kaempferol for 24 h...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29330945/cyclosporine-a-induces-endothelin-converting-enzyme-ece-1-studies-in-vivo-and-in-vitro
#3
Samuel N Heyman, Zaid Abassi, Christian Rosenberger, Hiba Yaseen, Galia Skarjinski, Ahuva Shina, Susanne Mathia, Natalia Krits, Mogher Khamaisi
AIM: Cyclosporine A (CsA) induces renal vasoconstriction and hypoxia and enhances the expression of endothelin-1 (ET-1) pro-hormone (pre-pro ET-1), plausibly leading to a feed-forward loop of renal vasoconstriction, hypoxia, and enhanced synthesis of the potent vasoconstrictor ET-1. Endothelin converting enzyme (ECE)-1 cleaves big endothelin to generate endothelin (ET)-1 and is up-regulated by hypoxia via hypoxia-inducible factor (HIF). We hypothesized that in addition to the direct induction of ET-1 synthesis, CsA might also intensify renal ECE-1expression, thus contributing to enhanced ET-1 synthesis following CsA...
January 13, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29330800/evaluation-of-erythrocyte-changes-after-normoxic-return-from-hypoxia
#4
Jihyun Song, Josef T Prchal
Hypoxia increases erythropoiesis by hypoxia-inducible factors (HIF), principally by HIF-2, which upregulates erythropoietin transcription. This results in an increase of red blood cell (RBC) production and delivery of more oxygen to tissues. Upon rapid return to normoxia, hypoxia-induced polycythemia is overcorrected by neocytolysis, a transient destruction of preferentially young RBCs bearing low catalase (downregulated by hypoxia-stimulated microRNA(miR)-21) caused by reactive oxygen species (ROS) from expanded mitochondria...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29330794/hypoxia-mediated-in-vivo-tumor-glucose-uptake-measurement-and-analysis
#5
Surendra K Shukla, Scott E Mulder, Pankaj K Singh
Most solid tumors are hypoxic in nature due to the limited supply of oxygen to internal tissues. Hypoxia plays an important role in metabolic adaptations of tumors that contribute significantly to cancer pathogenesis. Among the several metabolic alterations induced by hypoxia, hypoxia-mediated increased glucose uptake serves as the hallmark of metabolic reprogramming. Hypoxia-mediated stabilization of hypoxia-inducible factor-1 alpha (HIF-1α) transcription factor leads to altered expression of several glycolytic genes and glucose transporters, which results in increased glucose uptake by tumor cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29330786/kinetic-analysis-of-hif-prolyl-hydroxylases
#6
Peppi Koivunen, Johanna Myllyharju
Kinetic analyses of HIF prolyl 4-hydroxylases (HIF-P4Hs) allow determination of substrate, cosubstrate and cofactor requirements, analysis of the reaction rate, and inhibitory properties of the isoenzymes in vitro. Here we describe an assay measuring the substrate hydroxylation-coupled decarboxylation of radioactive 2-oxoglutarate to radioactive carbon dioxide as a fast, efficient, and diverse method to analyze the enzyme kinetics of HIF-P4Hs.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29328367/tumorigenic-proteins-upregulated-in-the-mycn-amplified-imr-32-human-neuroblastoma-cells-promote-proliferation-and-migration
#7
Hayat Zaatiti, Jad Abdallah, Zeina Nasr, George Khazen, Anthony Sandler, Tamara J Abou-Antoun
Childhood neuroblastoma is one of the most common types of extra-cranial cancer affecting children with a clinical spectrum ranging from spontaneous regression to malignant and fatal progression. In order to improve the clinical outcomes of children with high-risk neuroblastoma, it is crucial to understand the tumorigenic mechanisms that govern its malignant behaviors. MYCN proto-oncogene, bHLH transcription factor (MYCN) amplification has been implicated in the malignant, treatment-evasive nature of aggressive, high-risk neuroblastoma...
January 4, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29327447/itraq-based-proteomic-analysis-explore-the-influence-of-hypoxia-on-the-proteome-of-dental-pulp-stem-cells-under-3d-culture
#8
Lei Dou, Qifang Yan, Panpan Liang, Pengfei Zhou, Yan Zhang, Ping Ji
Various approaches are used to improve the efficacy of mesenchymal stem cells(MSCs) therapy. Hypoxic preconditioning is one of the most commonly-applied strategy prior to MSCs transplantation for enhancing the therapeutic effect. Identifying the effect of hypoxic preconditioning is helpful for better application. However, the influence of hypoxia on dental tissue derived MSCs cultured in 3D was lacking. Thus, this present study is to investigate the gene expression and proteome of dental pulp stem cells(DPSCs) after hypoxic preconditioning...
January 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29319128/the-preliminary-study-on-expression-and-clinical-value-of-platelet-derived-growth-factor-bb-hypoxia-inducible-factor-1%C3%AE-and-c-c-motif-chemokine-receptor-2-in-the-peripheral-blood-during-the-pathogenesis-of-graves-disease
#9
Ying Liu, Jinglan Tang, Qiaohong Hu, Kefeng Lu, Chunjie Hou
Backgroud: Platelet-derived growth factor BB (PDGF-BB) plays an important role in the development of GD(Graves' disease). However, it is still unknown whether PDGF-BB is expressed in peripheral blood and whether the expression of PDGF-BB contributes to GD. We aim to study the expression of PDGF-BB, Hypoxia inducible factor(HIF)-1α and C-C motif chemokine receptor(CCR)-2 in peripheral blood of patients with GD and explore its effect and potential mechanism in pathogenesis. METHODS: 41 patients with GD (GD group) and 45 healthy people(control group) were chosen...
January 10, 2018: Endokrynologia Polska
https://www.readbyqxmd.com/read/29307991/effects-of-initiating-time-and-dosage-of-panax-notoginseng-on-mucosal-microvascular-injury-in-experimental-colitis
#10
Shi-Ying Wang, Ping Tao, Hong-Yi Hu, Jian-Ye Yuan, Lei Zhao, Bo-Yun Sun, Wang-Jun Zhang, Jiang Lin
AIM: To investigate the effects of Panax notoginseng (PN) on microvascular injury in colitis, its mechanisms, initial administration time and dosage. METHODS: Dextran sodium sulfate (DSS)- or iodoacetamide (IA)-induced rat colitis models were used to evaluate and investigate the effects of ethanol extract of PN on microvascular injuries and their related mechanisms. PN administration was initiated at 3 and 7 d after the model was established at doses of 0.5, 1.0 and 2...
December 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29307990/polymorphisms-in-oxidative-pathway-related-genes-and-susceptibility-to-inflammatory-bowel-disease
#11
Nezha Senhaji, Sellama Nadifi, Younes Zaid, Aurora Serrano, Daniel Arturo Leon Rodriguez, Nadia Serbati, Mehdi Karkouri, Wafaa Badre, Javier Martín
AIM: To investigate whether common variants in the oxidative pathway genes influence inflammatory bowel disease (IBD) risk among Moroccan patients. METHODS: The distribution of (TAAA)n_rs12720460 and (CCTTT)n _rs3833912 NOS2A microsatellite repeats, HIF-1A_rs11549467 and NFKB1-94ins/delATTG_rs28362491 was analyzed in 507 subjects grouped in 199 IBD and 308 healthy controls. Genotyping was performed with polymerase chain reaction-fluorescent method and the TaqMan® allelic discrimination technology...
December 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29305422/tumor-stressors-induce-two-mechanisms-of-intracellular-p-glycoprotein-mediated-resistance-that-are-overcome-by-lysosomal-targeted-thiosemicarbazones
#12
Lina Al-Akra, Dong-Hun Bae, Sumit Sahni, Michael L H Huang, Kyung Chan Park, Darius J R Lane, Patric J Jansson, Des R Richardson
Multi-drug resistance (MDR) is a major obstacle in cancer treatment due to the ability of tumor cells to efflux chemotherapeutics via drug transporters e.g., P-glycoprotein (Pgp; ABCB1). While the mechanism of Pgp-mediated drug efflux is known at the plasma membrane, the functional role of intracellular Pgp is unclear. Moreover, there has been intense focus on the tumor micro-environment as a target for cancer treatment. This investigation aimed to dissect the effects of tumor micro-environmental stress on sub-cellular Pgp expression, localization and its role in MDR...
January 5, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29304733/cobalt-chloride-compromises-transepithelial-barrier-properties-of-caco-2-bbe-human-gastrointestinal-epithelial-cell-layers
#13
K M DiGuilio, M C Valenzano, E Rybakovsky, J M Mullin
BACKGROUND: Elevation of the transcription factor HIF-1 is a prominent mediator of not only processes that accompany hypoxia, but also the tumor microenvironment and tissue regeneration. This study uses mediators of "chemical hypoxia" to ask the question whether HIF-1α elevation in a healthy epithelial cell layer leads to leakiness in its tight junctional seals. METHODS: Transepithelial electrical resistance and transepithelial diffusion of 14C-D-mannitol and other radiolabeled probes are used as indicators of transepithelial barrier function of CaCo-2 BBe human gastrointestinal epithelial cell layers cultured on permeable supports...
January 5, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29295858/hif-1%C3%AE-stabilization-reduces-retinal-degeneration-in-a-mouse-model-of-retinitis-pigmentosa
#14
Lorena Olivares-González, Cristina Martínez-Fernández de la Cámara, David Hervás, José María Millán, Regina Rodrigo
Retinitis pigmentosa (RP) is a group of inherited retinal dystrophies characterized by progressive and irreversible loss of vision due to rod and cone degeneration. Evidence suggests that an inappropriate oxygen level could contribute to its pathogenesis. Rod cell death could increase oxygen concentration, reduce hypoxia-inducible factor 1 (HIF-1α) and contribute to cone cell death. The purposes of this study were: 1) to analyze the temporal profile of HIF-1α, its downstream effectors VEGF, endothelin-1 (ET-1), iNOS, and glucose transporter 1 (GLUT1), and neuroinflammation in retinas of the murine model of rd10 (retinal degeneration 10) mice with RP; 2) to study oxygen bioavailability in these retinas; and 3) to investigate how stabilizing HIF-1α proteins with dimethyloxaloglycine (DMOG), a prolyl hydroxylase inhibitor, affects retinal degeneration, neuroinflammation, and antioxidant response in rd10 mice...
January 2, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29295567/minoxidil-induction-of-vegf-is-mediated-by-inhibition-of-hif-prolyl-hydroxylase
#15
Soohwan Yum, Seongkeun Jeong, Dohoon Kim, Sunyoung Lee, Wooseong Kim, Jin-Wook Yoo, Jung-Ae Kim, Oh Sang Kwon, Dae-Duk Kim, Do Sik Min, Yunjin Jung
The topical application of minoxidil may achieve millimolar concentrations in the skin. We investigated whether millimolar minoxidil could induce vascular endothelial growth factor (VEGF), a possible effector for minoxidil-mediated hair growth, and how it occurred at the molecular level. Cell-based experiments were performed to investigate a molecular mechanism underlying the millimolar minoxidil induction of VEGF. The inhibitory effect of minoxidil on hypoxia-inducible factor (HIF) prolyl hydroxylase-2 (PHD-2) was tested by an in vitro von Hippel-Lindau protein (VHL) binding assay...
December 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29287795/hif-1%C3%AE-l-pgds-ppar%C3%AE-regulates-hypoxia-induced-anp-secretion-in-beating-rat-atria
#16
Xiang Li, Ying Zhang, Bo Zhang, Xia Liu, Lan Hong, Li-Ping Liu, Cheng-Zhe Wu, Xun Cui
Lipocalin-type prostaglandin D synthase (L-PGDS) and peroxisome proliferator activated receptor γ (PPARγ) play important roles in cardiovascular diseases. Nevertheless, effects of hypoxia-inducible factor 1α (HIF-1α) on L-PGDS and PPARγ protein levels and its role in hypoxia-induced atrial natriuretic peptide (ANP) secretion are unclear. In perfused beating rat atria, we observed that hypoxia significantly increased HIF-1α protein levels and stimulated ANP secretion, while upregulating L-PGDS. Hypoxia-induced ANP secretion was clearly attenuated by HIF-1α antagonist 2-methoxyestradiol, downregulating both HIF-1α and L-PGDS protein levels...
December 26, 2017: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/29287724/paris-reprograms-glucose-metabolism-by-hif-1%C3%AE-induction-in-dopaminergic-neurodegeneration
#17
Hojin Kang, Areum Jo, Hyein Kim, Rin Khang, Ji-Yeong Lee, Hanna Kim, Chi-Hu Park, Jeong-Yun Choi, Yunjong Lee, Joo-Ho Shin
Our previous study found that PARIS (ZNF746) transcriptionally suppressed transketolase (TKT), a key enzyme in pentose phosphate pathway (PPP) in the substantia nigra (SN) of AAV-PARIS injected mice. In this study, we revealed that PARIS overexpression reprogrammed glucose metabolic pathway, leading to the increment of glycolytic proteins along with TKT reduction in the SN of AAV-PARIS injected mice. Knock-down of TKT in differentiated SH-SY5Y cells led to an increase of glycolytic enzymes and decrease of PPP-related enzymes whereas overexpression of TKT restored PARIS-mediated glucose metabolic shift, suggesting that glucose metabolic alteration by PARIS is TKT-dependent...
December 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29284117/knockdown-of-ampk%C3%AE-decreases-atm-expression-and-increases-radiosensitivity-under-hypoxia-and-nutrient-starvation-in-an-sv40-transformed-human-fibroblast-cell-line-lm217
#18
Yasuhiko Murata, Takuma Hashimoto, Yusuke Urushihara, Soichiro Shiga, Kazuya Takeda, Keiichi Jingu, Yoshio Hosoi
BACKGROUND: Presence of unperfused regions containing cells under hypoxia and nutrient starvation contributes to radioresistance in solid human tumors. It is well known that hypoxia causes cellular radioresistance, but little is known about the effects of nutrient starvation on radiosensitivity. We have reported that nutrient starvation induced decrease of mTORC1 activity and decrease of radiosensitivity in an SV40-transformed human fibroblast cell line, LM217, and that nutrient starvation induced increase of mTORC1 activity and increase of radiosensitivity in human liver cancer cell lines, HepG2 and HuH6 (Murata et al...
December 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29282885/effects-of-varying-degrees-of-partial-bladder-outlet-obstruction-on-urinary-bladder-function-of-rats-a-novel-link-to-inflammation-oxidative-stress-and-hypoxia
#19
Ecem Kaya Sezginer, Didem Yilmaz-Oral, Utku Lokman, Serpil Nebioglu, Fugen Aktan, Serap Gur
OBJECTIVES: The aim of the present study was to investigate the effects of different degrees of obstruction, and the roles of inflammation, oxidative stress, and hypoxia parameters on bladder function. METHODS: Thirty male Sprague-Dawley rats were divided into 3 groups (n = 10 in each group): (i) sham-operated control; (ii) severe partial bladder outlet obstruction (PBOO); and (iii) moderate PBOO. Severe and moderate PBOO were induced by urethral ligation using 3-Fr and 4-Fr catheters, respectively, for 6 weeks...
December 28, 2017: Lower Urinary Tract Symptoms
https://www.readbyqxmd.com/read/29281714/coordinate-regulation-of-stress-signaling-and-epigenetic-events-by-acss2-and-hif-2-in-cancer-cells
#20
Rui Chen, Min Xu, Jason Nagati, Joseph A Garcia
Survival of cancer cells in the harsh tumor microenvironment, characterized by oxygen and glucose deprivation, requires rapid initiation of cytoprotective measures. Metabolites whose levels change during stress are ideal signaling cues, particularly if used in post-translational modifications of stress-responsive signal transducers. In cancer cells exposed to oxygen or glucose deprivation, there is an increase in cellular levels of acetate, a substrate for acetate-dependent acetyl CoA synthetase 2 (Acss2) that also stimulates translocation of Acss2 from the cytosol to the nucleus...
2017: PloS One
keyword
keyword
22676
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"