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Dua's layer

H S Dua, D G Said
PurposeTo design and produce a clamp that enables good handling of donor sclera-corneal disks, allows air to be injected in the corneal stroma and consistently provides pre-Descemets endothelial keratoplasty (PDEK) tissue by creation of a type-1 big bubble (BB) avoiding a type-2BB by shutting the fenestrations in the periphery of the pre-Descemets layer and preventing escape of air.Patients and methodsA clamp with spring loaded handles attached to two rings of 9 mm diameter and 1 mm width with a side port for insertion of a needle for air injection was designed and produced...
February 17, 2017: Eye
Yusuf Koçluk, Ayşe Burcu, Emine Alyamaç Sukgen
The authors aimed to present a deep anterior lamellar keratoplasty (DALK) surgery case with mixed type bubble demonstrating Dua's layer (DL). This was a reported case of DALK surgery. The authors encountered cornea DL structure at DALK surgery while cleaning the remaining stromal pieces. We also observed perforation in the central part of DL. However, DALK surgery could be completed. It is possible to encounter DL in a DALK surgery performed with mixed type big-bubble.
September 2016: Oman Journal of Ophthalmology
Yoshinori Oie, Kohji Nishida
PURPOSE OF REVIEW: Corneal triple procedures consist of combined keratoplasty surgery and cataract surgery. Currently, anterior lamellar keratoplasty, endothelial keratoplasty, and conventional penetrating keratoplasty, are very often simultaneously performed with cataract surgery. The present article reviews the recent advances and current developments in corneal triple procedures. RECENT FINDINGS: Many new findings have been reported. Core vitrectomy before host trephination and retroillumination during continuous curvilinear capsulorhexis has enabled us to perform penetrating keratoplasty triple procedures more safely...
January 2017: Current Opinion in Ophthalmology
S L AlTaan, K Termote, M S Elalfy, E Hogan, R Werkmeister, L Schmetterer, S Holland, H S Dua
PurposeTo define optical coherence tomography (OCT) characteristics of type-1, type-2, and mixed big bubbles (BB) seen in deep anterior lamellar keratoplasty.MethodsHuman sclero-corneal discs were obtained from UK (30) and Canada (16) eye banks. Air was injected into corneal stroma until a BB formed. UK samples were fixed in formalin before scanning with Fourier-domain (FD-OCT). One pair of each type of BB was scanned fresh. All BB obtained from Canada were scanned fresh with time-domain (TD-OCT). For each OCT machine used, type-1 BB from which Descemets membrane (DM) was partially peeled, were also scanned...
November 2016: Eye
A M Yeung, L A Faraj, O D McIntosh, V K Dhillon, H S Dua
PurposeFibrin glue has been used successfully in numerous ophthalmic surgical procedures. Recently, fibrin glue has been used in limbal stem cell transplantation to reduce both operative time and to negate the need for sutures. The aim of this study was to determine the effects of fibrin glue on epithelial cell migration in vitro.MethodsCorneoscleral rims were split to retain the epithelial layer, Bowman's layer, and anterior stroma. Rims were cut into eight equal-sized pieces and were placed directly on culture plates or affixed with fibrin glue...
October 2016: Eye
Kulwinder S Dua, Walter J Hogan, Abdul A Aadam, Mario Gasparri
BACKGROUND: Tissue-engineered extracellular matrix populated with autologous pluripotent cells can result in de-novo organogenesis, but the technique is complex, not widely available, and has not yet been used to repair large oesophageal defects in human beings. We aimed to use readily available stents and extracellular matrix to regenerate the oesophagus in vivo in a human being to re-establish swallowing function. METHODS: In a patient aged 24 years, we endoscopically placed a readily available, fully covered, self-expanding, metal stent (diameter 18 mm, length 120 mm) to bridge a 5 cm full-thickness oesophageal segment destroyed by a mediastinal abscess and leading to direct communication between the hypopharynx and the mediastinum...
July 2, 2016: Lancet
H S Dua, D G Said
No abstract text is available yet for this article.
August 2016: Eye
Harminder S Dua, Karolien Termote, Mohamed B Kenawy, Dalia G Said, Rakesh Jayaswal, Mario Nubile, Leonardo Mastropasqua, Simon Holland
PURPOSE: To evaluate the scrolling propensity of pre-Descemet endothelial keratoplasty (PDEK) tissue and to compare it with each component of the PDEK tissue, namely the pre-Descemet layer (Dua's layer [PDL]) and the Descemet membrane (DM). DESIGN: Experimental laboratory investigation. METHODS: Fourteen human donor sclerocorneal discs in which a type 1 big bubble was obtained by stromal injection of air were studied. The wall of the type 1 big bubble was excised and its grade of scrolling noted...
June 2016: American Journal of Ophthalmology
C Costet, D Touboul
No abstract text is available yet for this article.
April 2016: Journal Français D'ophtalmologie
Rupak Dua, Kristin Comella, Ryan Butler, Glenda Castellanos, Bryn Brazille, Andrew Claude, Arvind Agarwal, Jun Liao, Sharan Ramaswamy
We investigated the effectiveness of integrating tissue engineered cartilage derived from human bone marrow derived stem cells (HBMSCs) to healthy as well as osteoarthritic cartilage mimics using hydroxyapatite (HA) nanoparticles immersed within a hydrogel substrate. Healthy and diseased engineered cartilage from human chondrocytes (cultured in agar gels) were integrated with human bone marrow stem cell (HBMSC)-derived cartilaginous engineered matrix with and without HA, and evaluated after 28 days of growth...
2016: PloS One
Sherif A GamalElDin, Marwa M Salama, Malak I ElShazly
PURPOSE: To evaluate the efficacy of big-bubble (BB) technique in separating Dua's layer together with the Descemet's membrane endothelial (DE) graft and the effect of 7-day storage of the prepared tissues on the endothelial cells. METHOD: This is an experimental study in which 21 human corneo-scleral tissues unsuitable for transplantation were used. Grafts were mounted on artificial chamber; epithelial side-up with BB technique was used to detach the DE graft. The resultant tissues were stored in tissue culture medium for 7 days...
March 2016: Acta Ophthalmologica
Harminder S Dua, Leonardo Mastropasqua, Lana Faraj, Mario Nubile, Mohamed S Elalfy, Manuela Lanzini, Roberta Calienno, Dalia G Said
PURPOSE: In big bubble (BB), deep anterior lamellar keratoplasty intracorneal injection of air separates Descemet's membrane (DM) and the pre-Descemet's layer (Dua's layer [DL]) to create a type 1 BB. We tested the hypothesis that air injection after excision or ablation of DL will fail to produce a BB. METHODS: Nine human sclero-corneal discs were used. Three served as controls. In three, a type 1 BB was created, the bubble wall was excised and more air injected in an attempt to create another BB...
August 2015: Acta Ophthalmologica
Ankur Dua, Deepti Dua
BACKGROUND: The purpose of this study was to compare the efficacy of EndoVac irrigation system and side-vented closed ended needle (Max-I probe) in removing smear layer from root canals at 1 mm and 3 mm from working length using ProTaper rotary instrumentation. MATERIALS AND METHODS: A total of 50 freshly extracted maxillary central incisors were randomly divided into two groups after complete cleaning and shaping with ProTaper rotary files. In one group, final irrigation was performed with EndoVac system while in other group, final irrigation was done with a 30 gauge Max-I probe...
January 2015: Dental Research Journal
A A Zaki, M S Elalfy, D G Said, H S Dua
PURPOSE: To describe a technique which exploits the transparency and toughness of the pre-Descemets layer (Dua's layer) to safely perform deep anterior lamellar keratoplasty (DALK) and phacoemulsification at the same time. METHODS: Three DALK procedures combined with phacoemulsification were performed by the same surgeon using the big-bubble (BB) technique at the Research Institute of Ophthalmology, Cairo, Egypt. In two cases a type-1 BB, baring Dua's layer, was achieved and in one case a type-2 BB, baring the Descemet's membrane (DM), was achieved...
March 2015: Eye
Juma Z Hamad, Rubal Dua, Novita Kurniasari, Maria D Kennedy, Peng Wang, Gary L Amy
In this work, dual-templated hierarchical porous carbons (HPCs), produced from a coupled ice-hard templating approach, are shown to be a highly effective solution to the commonly occurring problem of irreversible fouling of low-pressure membranes used for pre-treatment in wastewater reuse. For the first time, dual-templated HPCs, along with their respective counterparts - single-templated meso-porous carbon (MPCs) (without macropores) - are tested in terms of their fouling reduction capacity and ability to remove different effluent organic matter fractions present in wastewater and compared with a commercially available powdered activated carbon (PAC)...
November 15, 2014: Water Research
Sepehr Feizi, Amir Faramarzi, Mohammad Ali Javadi, Mohammad Reza Jafarinasab
PURPOSE: To introduce a modification to big-bubble deep anterior lamellar keratoplasty (DALK) which can decrease the risk of Descemet membrane (DM) perforation during air injection. METHOD: In Anwar's big-bubble technique, a 27-guage needle is inserted from the trephination site and advanced into the corneal stroma up to the centre. The technique we introduce modifies the original technique in the following fashion. After trephination to approximately 80% of corneal thickness, a 27-guage needle is inserted into the stroma peripherally from the trephination site towards the limbus...
November 2014: British Journal of Ophthalmology
Deepti Dua, Ankur Dua, Veerendra M Uppin
AIM: The aim of this study was to compare smear layer removal at apical 1 mm level after final irrigation activation with an EndoVac system and Max-I probe. MATERIALS AND METHODS: Fifty freshly extracted maxillary central incisors were randomly divided into two groups after completing cleaning and shaping with ProTaper rotary files. In one group, final irrigation was performed with an EndoVac system while in the other group final irrigation was performed with a 30 gauge Max-I probe...
January 2014: Contemporary Clinical Dentistry
Amar Agarwal, Harminder S Dua, Priya Narang, Dhivya A Kumar, Ashvin Agarwal, Soosan Jacob, Athiya Agarwal, Ankur Gupta
A new surgical technique for endothelial keratoplasty is described, in which the composite of pre-Descemet's layer (Dua's layer) with Descemet's membrane and endothelium is transplanted subsequent to the removal of the recipient's Descemet's membrane. The technique was performed in five eyes of five patients, with successful attachment of the graft and good postoperative visual recovery in all cases. Postoperative optical coherence tomography showed good graft attachment without interface abnormalities and a mean graft thickness was 28±5...
September 2014: British Journal of Ophthalmology
Harminder S Dua, Lana A Faraj, Matthew J Branch, Aaron M Yeung, Mohamed S Elalfy, Dalia G Said, Trevor Gray, James Lowe
BACKGROUND: The trabecular meshwork (TM) located at the angle of the anterior chamber of the eye contributes to aqueous drainage. A novel layer in the posterior part of the human cornea has recently been reported (the pre-Descemet's layer (Dua's layer (PDL)). We examined the peripheral part of this layer in relation to the origin of the TM. METHODS: The PDL and TM of 19 human donor eyes and one exenterated sample were studied. Samples were examined by light and electron microscopy (EM) for tissue architecture and by immunohistology for four matricellular proteins, five collagen types and CD34...
May 2014: British Journal of Ophthalmology
Harminder S Dua, Lana A Faraj, Dalia G Said, Trevor Gray, James Lowe
PURPOSE: To define and characterize a novel pre-Descemet's layer in the human cornea. DESIGN: Clinical and experimental study. PARTICIPANTS: We included 31 human donor sclerocorneal discs, including 6 controls (mean age, 77.7 years). METHODS: Air was injected into the stroma of donor whole globes (n = 4) and sclerocorneal discs (n = 21) as in the clinical deep anterior lamellar keratoplasty procedure with the big bubble (BB) technique...
September 2013: Ophthalmology
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