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Ck2 kinase yeast

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https://www.readbyqxmd.com/read/26577526/the-nuclear-fraction-of-protein-kinase-ck2-binds-to-the-upstream-stimulatory-factors-usfs-in-the-absence-of-dna
#1
Sarah Spohrer, Elitsa Y Dimova, Thomas Kietzmann, Mathias Montenarh, Claudia Götz
The functions of the upstream stimulatory factors USF1 and USF2 are, like those of other transcription factors, regulated by reversible phosphorylation. Besides many other kinases also protein kinase CK2 phosphorylates USF1 but not USF2. In a yeast-two-hybrid screen, however, the non-catalytic CK2β subunit of CK2 was identified as a binding partner of USF2. This surprising observation prompted us to investigate the CK2/USF interaction in more detail in the present study. By using immunofluorescence analyses as well as co-immunoprecipitations we found that USF1 and USF2 bound to CK2α and CK2β exclusively in the nucleus, though CK2β and to a minor amount CK2α were also present in the cytoplasm...
February 2016: Cellular Signalling
https://www.readbyqxmd.com/read/26329457/sir2-phosphorylation-through-camp-pka-and-ck2-signaling-inhibits-the-lifespan-extension-activity-of-sir2-in-yeast
#2
Woo Kyu Kang, Yeong Hyeock Kim, Hyun Ah Kang, Ki-Sun Kwon, Jeong-Yoon Kim
Silent information regulator 2 (Sir2), an NAD(+)-dependent protein deacetylase, has been proposed to be a longevity factor that plays important roles in dietary restriction (DR)-mediated lifespan extension. In this study, we show that the Sir2's role for DR-mediated lifespan extension depends on cAMP-PKA and casein kinase 2 (CK2) signaling in yeast. Sir2 partially represses the transcription of lifespan-associated genes, such as PMA1 (encoding an H(+)-ATPase) and many ribosomal protein genes, through deacetylation of Lys 16 of histone H4 in the promoter regions of these genes...
2015: ELife
https://www.readbyqxmd.com/read/25970584/differential-phosphorylation-of-rna-polymerase-iii-and-the-initiation-factor-tfiiib-in-saccharomyces-cerevisiae
#3
Jaehoon Lee, Robyn D Moir, Ian M Willis
The production of ribosomes and tRNAs for protein synthesis has a high energetic cost and is under tight transcriptional control to ensure that the level of RNA synthesis is balanced with nutrient availability and the prevailing environmental conditions. In the RNA polymerase (pol) III system in yeast, nutrients and stress affect transcription through a bifurcated signaling pathway in which protein kinase A (PKA) and TORC1 activity directly or indirectly, through downstream kinases, alter the phosphorylation state and function of the Maf1 repressor and Rpc53, a TFIIF-like subunit of the polymerase...
2015: PloS One
https://www.readbyqxmd.com/read/25724641/the-rice-ck2-kinase-regulates-trafficking-of-phosphate-transporters-in-response-to-phosphate-levels
#4
Jieyu Chen, Yifeng Wang, Fei Wang, Jian Yang, Mingxing Gao, Changying Li, Yingyao Liu, Yu Liu, Naoki Yamaji, Jian Feng Ma, Javier Paz-Ares, Laurent Nussaume, Shuqun Zhang, Keke Yi, Zhongchang Wu, Ping Wu
Phosphate transporters (PTs) mediate phosphorus uptake and are regulated at the transcriptional and posttranslational levels. In one key mechanism of posttranslational regulation, phosphorylation of PTs affects their trafficking from the endoplasmic reticulum (ER) to the plasma membrane. However, the kinase(s) mediating PT phosphorylation and the mechanism leading to ER retention of phosphorylated PTs remain unclear. In this study, we identified a rice (Oryza sativa) kinase subunit, CK2β3, which interacts with PT2 and PT8 in a yeast two-hybrid screen...
March 2015: Plant Cell
https://www.readbyqxmd.com/read/25631054/differential-phosphorylation-of-a-regulatory-subunit-of-protein-kinase-ck2-by-target-of-rapamycin-complex-1-signaling-and-the-cdc-like-kinase-kns1
#5
Manuel E Sanchez-Casalongue, Jaehoon Lee, Aviva Diamond, Scott Shuldiner, Robyn D Moir, Ian M Willis
Transcriptional regulation of ribosome and tRNA synthesis plays a central role in determining protein synthetic capacity and is tightly controlled in response to nutrient availability and cellular stress. In Saccharomyces cerevisiae, the regulation of ribosome and tRNA synthesis was recently shown to involve the Cdc-like kinase Kns1 and the GSK-3 kinase Mck1. In this study, we explored additional roles for these conserved kinases in processes connected to the target of rapamycin complex 1 (TORC1). We conducted a synthetic chemical-genetic screen in a kns1Δ mck1Δ strain and identified many novel rapamycin-hypersensitive genes...
March 13, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25429105/regulation-of-ceramide-synthase-by-casein-kinase-2-dependent-phosphorylation-in-saccharomyces-cerevisiae
#6
Tara Fresques, Brad Niles, Sofia Aronova, Huzefa Mogri, Taha Rakhshandehroo, Ted Powers
Complex sphingolipids are important components of eukaryotic cell membranes and, together with their biosynthetic precursors, including sphingoid long chain bases and ceramides, have important signaling functions crucial for cell growth and survival. Ceramides are produced at the endoplasmic reticulum (ER) membrane by a multicomponent enzyme complex termed ceramide synthase (CerS). In budding yeast, this complex is composed of two catalytic subunits, Lac1 and Lag1, as well as an essential regulatory subunit, Lip1...
January 16, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25252977/tyrosine-phosphorylation-of-histone-h2a-by-ck2-regulates-transcriptional-elongation
#7
Harihar Basnet, Xue B Su, Yuliang Tan, Jill Meisenhelder, Daria Merkurjev, Kenneth A Ohgi, Tony Hunter, Lorraine Pillus, Michael G Rosenfeld
Post-translational histone modifications have a critical role in regulating transcription, the cell cycle, DNA replication and DNA damage repair. The identification of new histone modifications critical for transcriptional regulation at initiation, elongation or termination is of particular interest. Here we report a new layer of regulation in transcriptional elongation that is conserved from yeast to mammals. This regulation is based on the phosphorylation of a highly conserved tyrosine residue, Tyr 57, in histone H2A and is mediated by the unsuspected tyrosine kinase activity of casein kinase 2 (CK2)...
December 11, 2014: Nature
https://www.readbyqxmd.com/read/25183013/phosphorylation-by-casein-kinase-2-facilitates-psh1-protein-assisted-degradation-of-cse4-protein
#8
Geetha S Hewawasam, Mark Mattingly, Swaminathan Venkatesh, Ying Zhang, Laurence Florens, Jerry L Workman, Jennifer L Gerton
Cse4 is the centromeric histone H3 variant in budding yeast. Psh1 is an E3 ubiquitin ligase that controls Cse4 levels through proteolysis. Here we report that Psh1 is phosphorylated by the Cka2 subunit of casein kinase 2 (CK2) to promote its E3 activity for Cse4. Deletion of CKA2 significantly stabilized Cse4. Consistent with phosphorylation promoting the activity of Psh1, Cse4 was stabilized in a Psh1 phosphodepleted mutant strain in which the major phosphorylation sites were changed to alanines. Phosphorylation of Psh1 did not control Psh1-Cse4 or Psh1-Ubc3(E2) interactions...
October 17, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24926745/identification-of-aluminium-transport-related-genes-via-genome-wide-phenotypic-screening-of-saccharomyces-cerevisiae
#9
Nay M Tun, Patrick J O'Doherty, Zhong-Hua Chen, Xi-Yang Wu, Trevor D Bailey, Cindy Kersaitis, Ming J Wu
Genome-wide screening using gene deletion mutants has been widely carried out with numerous toxicants including oxidants and metal ions. The focus of such studies usually centres on identifying sensitive phenotypes against a given toxicant. Here, we screened the complete collection of yeast gene deletion mutants (5047) with increasing concentrations of aluminium sulphate (0.4, 0.8, 1.6 and 3.2 mM) in order to discover aluminium (Al(3+)) tolerant phenotypes. Fifteen genes were found to be associated with Al(3+) transport because their deletion mutants exhibited Al(3+) tolerance, including lem3Δ, hal5Δ and cka2Δ...
August 2014: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/24903109/receptor-mediated-mitophagy-in-yeast-and-mammalian-systems
#10
REVIEW
Lei Liu, Kaori Sakakibara, Quan Chen, Koji Okamoto
Mitophagy, or mitochondria autophagy, plays a critical role in selective removal of damaged or unwanted mitochondria. Several protein receptors, including Atg32 in yeast, NIX/BNIP3L, BNIP3 and FUNDC1 in mammalian systems, directly act in mitophagy. Atg32 interacts with Atg8 and Atg11 on the surface of mitochondria, promoting core Atg protein assembly for mitophagy. NIX/BNIP3L, BNIP3 and FUNDC1 also have a classic motif to directly bind LC3 (Atg8 homolog in mammals) for activation of mitophagy. Recent studies have shown that receptor-mediated mitophagy is regulated by reversible protein phosphorylation...
July 2014: Cell Research
https://www.readbyqxmd.com/read/24308916/identification-of-the-phosphorylated-residues-in-tveif5a-by-mass-spectrometry
#11
Laura Itzel Quintas-Granados, César López-Camarillo, Jesús Fandiño Armas, Guillermo Mendoza Hernandez, María Elizbeth Alvarez-Sánchez
The initiation factor eIF5A in Trichomonas vaginalis (TveIF5A) is previously shown to undergo hypusination, phosphorylation and glycosylation. Three different pI isoforms of TveIF5A have been reported. The most acidic isoform (pI 5.2) corresponds to the precursor TveIF5A, whereas the mature TveIF5A appears to be the most basic isoform (pI 5.5). In addition, the intermediary isoform (pI 5.3) is found only under polyamine-depleted conditions and restored with exogenous putrescine. We propose that differences in PI are due to phosphorylation of the TveIF5A isoforms...
December 2013: Genomics, Proteomics & Bioinformatics
https://www.readbyqxmd.com/read/24058532/detection-of-phospho-sites-generated-by-protein-kinase-ck2-in-cftr-mechanistic-aspects-of-thr1471-phosphorylation
#12
Andrea Venerando, Cinzia Franchin, Natasha Cant, Giorgio Cozza, Mario A Pagano, Kendra Tosoni, Ateeq Al-Zahrani, Giorgio Arrigoni, Robert C Ford, Anil Mehta, Lorenzo A Pinna
By mass spectrometry analysis of mouse Cystic Fibrosis Transmembrane-conductance Regulator (mCFTR) expressed in yeast we have detected 21 phosphopeptides accounting for 22 potential phospho-residues, 12 of which could be unambiguously assigned. Most are conserved in human CFTR (hCFTR) and the majority cluster in the Regulatory Domain, lying within consensus sequences for PKA, as identified in previous mammalian studies. This validates our yeast expression model. A number of phospho-residues were novel and human conserved, notably mouse Ser670, Ser723, Ser737, and Thr1467, that all lie in acidic sequences, compatible with their phosphorylation by protein kinase CK2...
2013: PloS One
https://www.readbyqxmd.com/read/23979598/casein-kinase-2-associates-with-the-yeast-chromatin-reassembly-factor-spt2-sin1-to-regulate-its-function-in-the-repression-of-spurious-transcription
#13
Wajid Bhat, Geneviève Boutin, Anne Rufiange, Amine Nourani
Spt2/Sin1 is a DNA binding protein with HMG-like domains. It plays a role in chromatin modulations associated with transcription elongation in Saccharomyces cerevisiae. Spt2 maintains the nucleosome level in coding regions and is important for the inhibition of spurious transcription in yeast. In this work, we undertook a biochemical approach to identify Spt2-interacting partners. Interestingly, casein kinase 2 (CK2) interacts with Spt2 and phosphorylates it in vitro as well as in vivo on two small regions, region I (RI) (amino acids 226 to 230) and RII (amino acids 277 to 281), located in its essential C-terminal domain...
November 2013: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/23897086/casein-kinase-2-is-essential-for-mitophagy
#14
Tomotake Kanki, Yusuke Kurihara, Xiulian Jin, Tadahiro Goda, Yusuke Ono, Masamune Aihara, Yuko Hirota, Tetsu Saigusa, Yoshimasa Aoki, Takeshi Uchiumi, Dongchon Kang
Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119...
September 2013: EMBO Reports
https://www.readbyqxmd.com/read/22810236/recovery-of-rna-polymerase-iii-transcription-from-the-glycerol-repressed-state-revisiting-the-role-of-protein-kinase-ck2-in-maf1-phosphoregulation
#15
Robyn D Moir, Jaehoon Lee, Ian M Willis
Maf1 is a conserved regulator of RNA polymerase (pol) III transcription and is required for transcriptional repression under diverse stress conditions. In yeast, Maf1 function is negatively regulated at seven phosphosites by the overlapping action of protein kinase A (PKA) and the TORC1-regulated kinase Sch9. Under stress conditions, Maf1 is dephosphorylated at these sites leading to its nuclear accumulation, increased association with pol III genes and direct physical interactions with the polymerase which ultimately inhibit transcription...
August 31, 2012: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/22789442/dual-function-of-pin1-in-nr4a-nuclear-receptor-activation-enhanced-activity-of-nr4as-and-increased-nur77-protein-stability
#16
Claudia M van Tiel, Kondababu Kurakula, Duco S Koenis, Erik van der Wal, Carlie J M de Vries
Nur77, Nurr1 and NOR-1 form the NR4A subfamily of the nuclear receptor superfamily and have been shown to regulate various biological processes among which are cell survival and differentiation, apoptosis, inflammation and metabolism. These nuclear receptors have been proposed to act in a ligand-independent manner and we aim to gain insight in the regulation of NR4A activity. A yeast two-hybrid screen identified the peptidyl-prolyl isomerase Pin1 as a novel binding partner of NR4As, which was confirmed by co-immunoprecipitation...
October 2012: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/22695718/casein-kinase-2%C3%AE-regulates-multidrug-resistance-associated-protein-1-function-via-phosphorylation-of-thr249
#17
Elzbieta I Stolarczyk, Cassandra J Reiling, Kerry A Pickin, Ryan Coppage, Marc R Knecht, Christian M Paumi
We have shown previously that the function of Ycf1p, yeast ortholog of multidrug resistance-associated protein 1 (MRP1), is regulated by yeast casein kinase 2α (Cka1p) via phosphorylation at Ser251. In this study, we explored whether casein kinase 2α (CK2α), the human homolog of Cka1p, regulates MRP1 by phosphorylation at the semiconserved site Thr249. Knockdown of CK2α in MCF7-derived cells expressing MRP1 [MRP1 CK2α(-)] resulted in increased doxorubicin sensitivity. MRP1-dependent transport of leukotriene C(4) and estradiol-17β-d-glucuronide into vesicles derived from MRP1 CK2α(-) cells was decreased compared with MRP1 vesicles...
September 2012: Molecular Pharmacology
https://www.readbyqxmd.com/read/22438817/mapping-the-hsp90-genetic-interaction-network-in-candida-albicans-reveals-environmental-contingency-and-rewired-circuitry
#18
Stephanie Diezmann, Magali Michaut, Rebecca S Shapiro, Gary D Bader, Leah E Cowen
The molecular chaperone Hsp90 regulates the folding of diverse signal transducers in all eukaryotes, profoundly affecting cellular circuitry. In fungi, Hsp90 influences development, drug resistance, and evolution. Hsp90 interacts with -10% of the proteome in the model yeast Saccharomyces cerevisiae, while only two interactions have been identified in Candida albicans, the leading fungal pathogen of humans. Utilizing a chemical genomic approach, we mapped the C. albicans Hsp90 interaction network under diverse stress conditions...
2012: PLoS Genetics
https://www.readbyqxmd.com/read/22156209/restriction-of-histone-gene-transcription-to-s-phase-by-phosphorylation-of-a-chromatin-boundary-protein
#19
Christoph F Kurat, Jean-Philippe Lambert, Dewald van Dyk, Kyle Tsui, Harm van Bakel, Supipi Kaluarachchi, Helena Friesen, Pinay Kainth, Corey Nislow, Daniel Figeys, Jeffrey Fillingham, Brenda J Andrews
The cell cycle-regulated expression of core histone genes is required for DNA replication and proper cell cycle progression in eukaryotic cells. Although some factors involved in histone gene transcription are known, the molecular mechanisms that ensure proper induction of histone gene expression during S phase remain enigmatic. Here we demonstrate that S-phase transcription of the model histone gene HTA1 in yeast is regulated by a novel attach-release mechanism involving phosphorylation of the conserved chromatin boundary protein Yta7 by both cyclin-dependent kinase 1 (Cdk1) and casein kinase 2 (CK2)...
December 1, 2011: Genes & Development
https://www.readbyqxmd.com/read/21816617/rna-polymerase-iii-under-control-repression-and-de-repression
#20
Magdalena Boguta, Damian Graczyk
The synthesis of tRNA by yeast RNA polymerase III (Pol III) is regulated in response to changing environmental conditions. This control is mediated by Maf1, the global negative regulator of Pol III transcription conserved from yeast to humans. Details regarding the molecular basis of Pol III repression by Maf1 are now emerging from recently reported structural and biochemical data on Pol III and Maf1. Efficient Pol III transcription, following the shift of cells from a non-fermentable carbon source to glucose, requires phosphorylation of Maf1...
September 2011: Trends in Biochemical Sciences
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