Read by QxMD icon Read

Lactic acid production

Zhongyang Qiu, Qiuqiang Gao, Jie Bao
Xylose-assimilating pathways were constructed in the parental Pediococcus acidilactici strain and evolutionarily adapted to yield a highly stable co-fermentation strain for l-lactic acid production. The phosphoketolase pathway (PK) was blocked for reduction of acetic acid generation by disrupting phosphoketolase (pkt) gene. The pentose phosphate pathway (PPP) was reconstructed for xylose assimilation by integrating four heterologous genes encoding transketolase (tkt), transaldolase (tal), xylose isomerase (xylA) and xylulokinase (xylB) into the P...
September 21, 2017: Bioresource Technology
Lei Mao, Anton S M Sonnenberg, Wouter H Hendriks, John W Cone
BACKGROUND: No attention has been paid so far to the preservation of fungal treated lignocellulose for longer periods. Here we treated wheat straw (WS) with the white-rot fungi Ceriporiopsis subvermispora and Lentinula edodes for 8 weeks and assessed changes in pH, chemical composition and in vitro gas production (IVGP) weekly. Fungal treated WS was also stored for 64 days as is, with addition of lactic acid bacteria (LAB) or with a combination of LAB and molasses in airtight glass jars mimicking ensiling conditions...
October 14, 2017: Journal of the Science of Food and Agriculture
Juan Borrero, Eoin Kelly, Paula M O'Connor, Philip Kelleher, Colm Scully, Paul D Cotter, Jennifer Mahony, Douwe van Sinderen
Bacteriocins from lactic acid bacteria (LAB) are of increasing interest in recent years due to their potential as natural preservatives against food and beverage spoilage microorganisms. In a screening study for LAB, we isolated a strain, Lactobacillus plantarum NI326, from olives with activity against a strain belonging to the beverage-spoilage bacterium Alicyclobacillus acidoterrestris spp. Genome sequencing of the strain enabled the identification of a gene cluster encoding a putative circular bacteriocin and proteins involved in its modification, transport and immunity...
October 13, 2017: Applied and Environmental Microbiology
Boyeon Park, Hyelyeon Hwang, Jina Lee, Sung-Oh Sohn, Se Hee Lee, Min Young Jung, Hyeong In Lim, Hae Woong Park, Jong-Hee Lee
BACKGROUND: Panax ginseng is a physiologically active plant widely used in traditional medicine that is characterized by the presence of ginsenosides. Rb1, a major ginsenoside, is used as the starting material for producing ginsenoside derivatives with enhanced pharmaceutical potentials through chemical, enzymatic, or microbial transformation. METHODS: To investigate the bioconversion of ginsenoside Rb1, we prepared kimchi originated bacterial strains Leuconostoc mensenteroides WiKim19, Pediococcus pentosaceus WiKim20, Lactobacillus brevis WiKim47, Leuconostoc lactis WiKim48, and Lactobacillus sakei WiKim49 and analyzed bioconversion products using LC-MS/MS mass spectrometer...
October 2017: Journal of Ginseng Research
Shiren Sun, Heng Li, Jianghua Chen, Qi Qian
For decades, lactic acid has been considered a dead-end product of glycolysis. Research in the last 20+ years has shown otherwise. Through its transporters (MCTs) and receptor (GPR81), lactic acid plays a key role in multiple cellular processes, including energy regulation, immune tolerance, memory formation, wound healing, ischemic tissue injury, and cancer growth and metastasis. We summarize key findings of lactic acid signaling, functions, and many remaining questions.
November 2017: Physiology
Katarina Veljović, Nikola Popović, Marija Miljković, Maja Tolinački, Amarela Terzić-Vidojević, Milan Kojić
The understanding of mechanisms of interactions between various bacterial cell surface proteins and host receptors has become imperative for the study of the health promoting features of probiotic enterococci. This study, for the first time, describes a novel enterococcal aggregation protein, AggE, from Enterococcus faecium BGGO9-28, selected from a laboratory collection of enterococcal isolates with auto-aggregation phenotypes. Among them, En. faecium BGGO9-28 showed the strongest auto-aggregation, adhesion to components of ECM and biofilm formation...
2017: Frontiers in Microbiology
Hee Min Lee, Ji-Hyun Lee, Sung Hyun Kim, So-Ra Yoon, Jae Yong Lee, Ji-Hyoung Ha
Recently, cabbage Kimchi have occasionally been associated with the food-borne diseases of enteric viruses such as human norovirus (HuNoV). This study aimed to evaluate the correlation between microbial/physicochemical properties and persistence of human norovirus in experimentally contaminated cabbage Kimchi fermented and stored at 4 °C or 10 °C for 28 days. Changes in organic acid content, lactic acid bacteria (LAB), acidity, pH, and salinity were analyzed. The recovery of structurally intact HuNoV was examined for up to 28 days post-inoculation, using a NoV GII...
October 11, 2017: Journal of Microbiology and Biotechnology
M M Soltan Dallal, S Zamaniahari, A Davoodabadi, M Hosseini, Z Rajabi
Background: The pickle, a traditional fermented product, is popular among Iranians. Much research has been conducted worldwide on this food group. Due to a lack of related data in Iran, this study was conducted to isolate and identify dominant lactic acid bacteria (LAB) in pickles and salted pickles. Materials and methods: Seventy samples were collected from different regions of Iran. The isolated bacteria were identified as LAB by Gram staining and catalase by using MRS agar. Then, those strains were identified at the species level by physiological tests (e...
2017: GMS Hygiene and Infection Control
Yiyuan Xue, Qian Lu, Yuan Tian, Xuedong Zhou, Lei Cheng, Biao Ren
OBJECTIVES: To evaluate the effects of 8% arginine-containing toothpaste on the dental plaque of no caries (NC) and high caries (HC) individuals in situ. METHODS: 6 no caries (DMFT=0) and 6 high caries (DMFT≥6) individuals wearing a self-developed in situ dental plaque acquisition device were involved in a randomized double-blinded crossover study for 6 weeks: including lead-in (1 week), arginine-free (2 weeks), washout (2 weeks) and arginine-active (2 weeks) stages...
October 5, 2017: Journal of Dentistry
Deepika Awasthi, Liang Wang Mun Su Rhee, Qingzhao Wang, Diane Chauliac, Lonnie O Ingram, K T Shanmugam
Poly lactic acid (PLA) based plastics is renewable, bio-based and biodegradable. Although present day PLA is composed of mainly L-LA, an L- and D- LA copolymer is expected to improve the quality of PLA and expand its use. To increase the number of thermotolerant microbial biocatalysts that produce D-LA, a derivative of Bacillus subtilis strain 168 that grows at 50°C was metabolically engineered. Since B. subtilis lacks a gene encoding D-lactate dehydrogenase (ldhA), five heterologous ldhA genes (B. coagulans ldhA and gldA101 and ldhA from three Lactobacillus delbrueckii) were evaluated...
October 7, 2017: Biotechnology and Bioengineering
Melinda A Engevik, James Versalovic
Commensal and beneficial microbes secrete myriad products which target the mammalian host and other microbes. These secreted substances aid in bacterial niche development, and select compounds beneficially modulate the host and promote health. Microbes produce unique compounds which can serve as signaling factors to the host, such as biogenic amine neuromodulators, or quorum-sensing molecules to facilitate inter-bacterial communication. Bacterial metabolites can also participate in functional enhancement of host metabolic capabilities, immunoregulation, and improvement of intestinal barrier function...
October 2017: Microbiology Spectrum
Yeun-Hwa Gu, Hyunju Choi, Takenori Yamashita, Ki-Mun Kang, Masahiro Iwasa, Moon-Jo Lee, Kyoung Hae Lee, Cheorl-Ho Kim
BACKGROUND: Enterococcus faecalis 2001 is a probiotic lactic acid bacterium and has been used as a biological response modifier (BRM). From physiological limitation of bacterial preservation in storage and safety, the live E. faecalis 2001 has been heat-treated and the BRM components containing high level of β-glucan, named EF-2001, has been prepared. METHOD: The heat-treated EF-2001 has been examined for the antioxidative potential for radical scavenging and anti-tumor activities as well as immune-enhancing response in mice...
October 2, 2017: Current Pharmaceutical Biotechnology
A Pandey, A Pandey
In this study photo-hydrogen production from cheese whey dark fermentation (DF) effluent by the co-culture of Rhodobacter sphaeroides -NMBL-01 and Bacillus firmus - NMBL-03 has been reported. The effect of pH, initial chemical oxygen demand (COD) and the concentration effect of FeSO4.7H2O on photo-hydrogen production have been investigated. The end products of dark fermentation effluent of cheese whey were mainly comprised of soluble organic acids, i.e. butyric acid and lactic acid. The batch process was carried out under light intensity of 2...
July 31, 2017: Cellular and Molecular Biology
Xudong Bao, Jingmei Yang, J J de Soet, Hongyan Liu, Xuejun Gao, Cor van Loveren, Dongmei Deng
Previous studies have shown that Streptococcus oligofermentans inhibits the growth of cariogenic Streptococcus mutans in biofilms in vitro and is considered a probiotic candidate for caries prevention. This study aimed to examine the effects of various environmental factors on the competition between S. oligofermentans and S. mutans in a dual-species biofilm model. Single or dual S. oligofermentans and S. mutans biofilms were grown in a 96-well active attachment model for 48 h. Several growth conditions were examined in the model, namely: S...
September 30, 2017: Caries Research
Sung-Ho Son, Hye-Lin Jeon, Seo-Jin Yang, Na-Kyoung Lee, Hyun-Dong Paik
This study aimed at evaluating the functional and probiotic properties of three lactic acid bacterial (LAB) strains isolated from kimchi. The selected LAB strains, which had potential probiotic functions, were identified by 16S rRNA sequence analysis as Lactobacillus brevis G1, L. brevis KU15006, and Lactobacillus curvatus KCCM 200173. All LAB strains were able to tolerate incubation at pH 2.5 with 0.3% pepsin for 3 h and with 0.3% Oxgall for 24 h and showed similar enzyme production levels, antimicrobial activities, and antibiotic susceptibilities...
September 28, 2017: Microbial Pathogenesis
Xu Jie, Haimei Yang, Min Wang, Yue Zhang, Weili Wei, Zhining Xia
Profiling of oxidase-catalyzed biomarkers is an essential procedure for the diagnosis and precise treatment of the metabolic diseases. Inspired by the metabolism of H2O2 in peroxisomes, a novel chemiluminescent silica nanodevice (CSN) was designed for sensitive and selective sensing of oxidase-catalyzed biomarkers. Oxidases catalyzed the oxidation of biomarkers followed by the production of H2O2, and then the generated H2O2 was employed as a messenger to trigger the chemiluminescence of the CSN. Utilizing this nanodevice, we not only accurately quantified intracellular glucose but also developed its further application for high throughput insulin sensitizer screening...
September 28, 2017: Angewandte Chemie
H B Feys, R Devloo, B Sabot, K De Pourcq, J Coene, V Compernolle
BACKGROUND: Pathogen inactivation methods for platelet concentrates are increasingly being used in blood banks worldwide. In vitro studies have demonstrated its effects on storage lesion, but little routine quality control data on blood banking outcomes have been reported. MATERIALS AND METHODS: Swirling of distributed products was monitored before and after implementation of Intercept pathogen inactivation. Metabolic parameters pH, glucose and lactic acid were determined in a random cohort of expired pathogen-inactivated products...
September 27, 2017: Vox Sanguinis
Gabriel Quintana, Maria V Niederle, Carlos J Minahk, Gianluca Picariello, María E F Nader-Macías, Sergio E Pasteris
Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria monocytogenes as well. Considering that probiotics requires high cell densities and/or bacteriocin concentrations, the effect of the temperature on L. lactis growth and bacteriocin production was evaluated to find the optimal conditions. Thus, the growth rate was maximal at 36 °C, whereas the highest biomass and bacteriocin activity was achieved between 20 and 30 °C and 20-25 °C, respectively...
September 27, 2017: World Journal of Microbiology & Biotechnology
Ilavenil Soundharrajan, Da Hye Kim, Srigopalram Srisesharam, Palaniselvam Kuppusamy, Hyung Soo Park, Yong Hee Yoon, Won Ho Kim, Young Gil Song, Ki Choon Choi
The present study aimed to investigate the efficacy of customised Lactobacillus plantarum KCC-10, KCC-19 and K-46 on nutrient composition and fermentation quality of low moisture Italian ryegrass (IRG) forage. An addition of customised bacterial inoculants (CBI) did not affect the nutrient compositions and digestibility rates of haylage. The lactic acid content was higher in CBI-inoculated haylage, whereas the amount of acetic acid and butyric acid production was significantly reduced than the control. CBI-inoculated haylage exhibited higher numbers of bacterial colonies that reduced the pH of the haylage...
October 2017: 3 Biotech
Mahboubeh Ebrahimian, Maryam Hashemi, Mohsen Maleki, Gholamreza Hashemitabar, Khalil Abnous, Mohammad Ramezani, Alireza Haghparast
Strategies to design delivery vehicles are critical in modern vaccine-adjuvant development. Nanoparticles (NPs) encapsulating antigen(s) and adjuvant(s) are promising vehicles to deliver antigen(s) and adjuvant(s) to antigen-presenting cells (APCs), allowing optimal immune responses against a specific pathogen. In this study, we developed a novel adjuvant delivery approach for induction of efficient in vivo immune responses. Polyethylenimine (PEI) was physically conjugated to poly(lactic-co-glycolic) acid (PLGA) to form PLGA/PEI NPs...
2017: Frontiers in Immunology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"