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Lactic acid production

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https://www.readbyqxmd.com/read/28444600/antibiotic-sensitivity-pattern-of-indigenous-lactobacilli-isolated-from-curd-and-human-milk-samples
#1
Chetan Sharma, Sachin Gulati, Nishchal Thakur, Brij Pal Singh, Sanjolly Gupta, Simranpreet Kaur, Santosh Kumar Mishra, Anil Kumar Puniya, Jatinder Pal Singh Gill, Harsh Panwar
The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may act as pool of antimicrobial resistance genes that could be transferred to pathogens, either in food matrix or in gastrointestinal tract, which could be detrimental to host. This study evaluated the antibiotic susceptibility patterns of LAB isolated from curd (20) and human milk (11) samples...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28439768/discrimination-of-wine-lactic-acid-bacteria-by-raman-spectroscopy
#2
Susan B Rodriguez, Mark A Thornton, Roy J Thornton
Species of Lactobacillus, Pediococcus, Oenococcus, and Leuconostoc play an important role in winemaking, as either inoculants or contaminants. The metabolic products of these lactic acid bacteria have considerable effects on the flavor, aroma, and texture of a wine. However, analysis of a wine's microflora, especially the bacteria, is rarely done unless spoilage becomes evident, and identification at the species or strain level is uncommon as the methods required are technically difficult and expensive. In this work, we used Raman spectral fingerprints to discriminate 19 strains of Lactobacillus, Pediococcus, and Oenococcus...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439299/substance-p-enhances-lactic-acid-and-tyramine-production-in-enterococcus-faecalis-v583-and-promotes-its-cytotoxic-effect-on-intestinal-caco-2-tc7-cells
#3
Kelly Biaggini, Valérie Borrel, Sabine Szunerits, Rabah Boukherroub, Awa N'Diaye, Arthur Zébré, Maryse Bonnin-Jusserand, Guillaume Duflos, Marc Feuilloley, Djamel Drider, Pierre Déchelotte, Nathalie Connil
BACKGROUND: Enterococcus faecalis, generally considered as a saprophytic bowel commensal, has recently emerged as an important nosocomial pathogen causing severe urinary tract infections, surgical wound infections, bacteremia, and bacterial endocarditis. This bacterium is capable of forming biofilms on various surfaces and its high level of antibiotic resistance contributes to its pathogenicity. The aim of this study was to evaluate the effect on E. faecalis, of Substance P (SP), an antimicrobial peptide that is produced in the gut and skin...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28438266/physiological-role-of-two-component-signal-transduction-systems-in-food-associated-lactic-acid-bacteria
#4
Vicente Monedero, Ainhoa Revilla-Guarinos, Manuel Zúñiga
Two-component systems (TCSs) are widespread signal transduction pathways mainly found in bacteria where they play a major role in adaptation to changing environmental conditions. TCSs generally consist of sensor histidine kinases that autophosphorylate in response to a specific stimulus and subsequently transfer the phosphate group to their cognate response regulators thus modulating their activity, usually as transcriptional regulators. In this review we present the current knowledge on the physiological role of TCSs in species of the families Lactobacillaceae and Leuconostocaceae of the group of lactic acid bacteria (LAB)...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/28438014/characterization-of-a-glutamate-decarboxylase-gad-from-enterococcus-avium-m5-isolated-from-jeotgal-a-fermented-korean-sea-food
#5
Kang Wook Lee, Jae Min Shim, Zhuang Yao, Jeong A Kim, Hyun-Jin Kim, Jeong Hwan Kim
To develop starters for the production of functional foods or materials, lactic acid bacteria producing γ-aminobutyric acid (GABA) were screened from jeotgals, Korean fermented sea foods. One isolate producing high amount of GABA from monosodium L-glutamate (MSG) was identified as Enterococcus avium by 16S rRNA gene sequencing. E. avium M5 produced 18.47 ± 1.26 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with MSG (3%, w/v). A gadB gene encoding glutamate decarboxylase (GAD) was cloned and overexpressed in E...
April 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28436165/production-of-high-concentration-of-l-lactic-acid-from-oil-palm-empty-fruit-bunch-by-thermophilic-b-coagulans-ji12
#6
Veeresh Juturu, Jin Chuan Wu
Thermophilic Bacillus coagulans JI12 was used to ferment hemicellulose hydrolysate obtained by acid hydrolysis of oil palm empty fruit bunch (EFB) at 50°C and pH6, producing 105.4 g/L of L-lactic acid with a productivity of 9.3 g/L/h by fed batch fermentation under unsterilized conditions. Simultaneous saccharification and fermentation (SSF) was performed at pH5.5 and 50°C to convert both hemicellulose hydrolysate and cellulose-lignin complex in the presence of Cellic Ctec2 cellulases using yeast extract (20 g/L) as the nitrogen source, giving 114...
April 24, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28435139/the-role-of-lactic-acid-production-by-probiotic-lactobacillus-species-in-vaginal-health
#7
Gilda Tachedjian, Muriel Aldunate, Catronia S Bradshaw, Richard A Cone
Vaginal eubiosis is characterised by beneficial lactobacillus-dominated microbiota. In contrast, vaginal dysbiosis (e.g. bacterial vaginosis, BV), characterised by an overgrowth of multiple anaerobes, is associated with an increased risk of adverse urogenital and reproductive health outcomes. A major distinguishing feature between the vaginal environment in states of eubiosis and dysbiosis is a high concentration of lactic acid, produced by lactobacilli, that acidifies the vagina in eubiosis versus a sharp drop in lactic acid and an increase in pH in dysbiosis...
April 20, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28434721/characterization-of-probiotic-bacteria-involved-in-fermented-milk-processing-enriched-with-folic-acid
#8
Zhen Wu, Jing Wu, Pei Cao, Yifeng Jin, Daodong Pan, Xiaoqun Zeng, Yuxing Guo
Yogurt products fermented with probiotic bacteria are a consumer trend and a challenge for functional food development. So far, limited research has focused on the behavior of the various probiotic strains used in milk fermentation. In the present study, we characterized folic acid production and the sensory and textural characteristics of yogurt products fermented with probiotic bacteria. Yogurt fermented with Lactobacillus plantarum had improved nutrient content and sensory and textural characteristics, but the presence of L...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434172/lactobacillus-paracasei-strain-06tca19-suppresses-inflammatory-chemokine-induced-by-helicobacter-pylori-in-human-gastric-epithelial-cells
#9
Shiro Takeda, Keiji Igoshi, Chuluunbat Tsend-Ayush, Tsendesuren Oyunsuren, Ryoichi Sakata, Yasuhiro Koga, Yuo Arima, Masahiko Takeshita
Helicobacter (H.) pylori infection is an important risk factor for gastric cancer that causes gastric inflammation. Inflammatory chemokines such as interleukin (IL)-8 and regulated on activation normal T cell expressed and secreted (RANTES) are elevated in the gastric mucosa by H. pylori. This study aimed to investigate the effects of Lactobacillus paracasei strain 06TCa19, a probiotic strain, on IL-8 and RANTES expression and production induced by H. pylori using human gastric epithelial cell lines. Strain 06TCa19 was shown to suppress H...
April 22, 2017: Human Cell
https://www.readbyqxmd.com/read/28432948/butanol-fermentation-of-the-brown-seaweed-laminaria-digitata-by-clostridium-beijerinckii-dsm-6422
#10
Xiaoru Hou, Nikolaj From, Irini Angelidaki, Wouter J J Huijgen, Anne-Belinda Bjerre
Seaweed represents an abundant, renewable, and fast-growing biomass resource for 3rd generation biofuel production. This study reports an efficient butanol fermentation process carried out by Clostridium beijerinckii DSM-6422 using enzymatic hydrolysate of the sugar-rich brown seaweed Laminaria digitata harvested from the coast of the Danish North Sea as substrate. The highest butanol yield (0.42g/g-consumed-substrates) compared to literature was achieved, with a significantly higher butanol:acetone-butanol-ethanol (ABE) molar ratio (0...
April 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28432440/application-of-recombinant-pediococcus-acidilactici-bd16-fcs-ech-for-bioconversion-of-agrowaste-to-vanillin
#11
Debkumar Chakraborty, Ammaiyappan Selvam, Baljinder Kaur, Jonathan Woon Chung Wong, Obulisamy Parthiba Karthikeyan
Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use of an agrobiomass waste, rice bran, and provides the first evidence of a cost-effective production of vanillin within 24 h of incubation using recombinant Pediococcus acidilactici BD16 (fcs (+)/ech (+))...
April 21, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28430940/unravelling-microbial-ecology-of-industrial-scale-kombucha-fermentations-by-metabarcoding-and-culture-based-methods
#12
M Coton, A Pawtowski, B Taminiau, G Burgaud, F Deniel, L Coulloume-Labarthe, P A Fall, G Daube, E Coton
Kombucha, historically an Asian tea-based fermented drink, has recently become trendy in Western countries. Producers claim it bears health-enhancing properties that may come from the tea or metabolites produced by its microbiome. Despite its long history of production, microbial richness and dynamics have not been fully unravelled, especially at an industrial scale. Moreover, the impact of tea type (green or black) on microbial ecology was not studied. Here, a study compared microbial communities from industrial scale black and green tea fermentations, still traditionally carried out by a microbial biofilm, using culture-dependent and metabarcoding approaches...
April 18, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28429764/fabrication-characterization-and-biological-evaluation-of-anti-her2-indocyanine-green-doxorubicin-encapsulated-peg-b-plga-copolymeric-nanoparticles-for-targeted-photochemotherapy-of-breast-cancer-cells
#13
Yu-Hsiang Lee, Da-Sheng Chang
In this study, we aimed to develop anti-human epidermal growth factor receptor 2 (HER2) indocyanine green (ICG)-doxorubicin (DOX)-encapsulated polyethylene glycol-poly(lactic-co-glycolic acid) diblock copolymeric nanoparticles (HIDPPNPs) to explore the co-administration of phototherapy and chemotherapy for HER2-overexpressing breast cancer, a highly aggressive and medicine-resistant breast carcinoma. The HIDPPNPs were fabricated using a solvent evaporation technique followed by carbodiimide-mediated antibody conjugation on the nanoparticle surface...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28429326/wax-ester-fermentation-and-its-application-for-biofuel-production
#14
Hiroshi Inui, Takahiro Ishikawa, Masahiro Tamoi
In Euglena cells under anaerobic conditions, paramylon, the storage polysaccharide, is promptly degraded and converted to wax esters. The wax esters synthesized are composed of saturated fatty acids and alcohols with chain lengths of 10-18, and the major constituents are myristic acid and myristyl alcohol. Since the anaerobic cells gain ATP through the conversion of paramylon to wax esters, the phenomenon is named "wax ester fermentation". The wax ester fermentation is quite unique in that the end products, i...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28420232/gas-phase-carboxylic-acids-in-a-university-classroom-abundance-variability-and-sources
#15
Shang Liu, Samantha L Thompson, Harald Stark, Paul J Ziemann, Jose L Jimenez
Gas-phase carboxylic acids are ubiquitous in ambient air, yet their indoor occurrence and abundance are poorly characterized. To fill this gap, we measured gas-phase carboxylic acids in real-time inside and outside of a university classroom using a high-resolution time-of-flight chemical ionization mass spectrometer (HRToF-CIMS) equipped with an acetate ion source. A wide variety of carboxylic acids were identified indoors and outdoors, including monoacids, diacids, hydroxy acids, carbonyl acids, and aromatic acids...
April 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28419329/effects-of-the-inclusion-of-a-bacillus-direct-fed-microbial-on-performance-parameters-bone-quality-recovered-gut-microflora-and-intestinal-morphology-in-broilers-consuming-a-grower-diet-containing-corn-distillers-dried-grains-with-solubles
#16
J D Latorre, X Hernandez-Velasco, J L Vicente, R Wolfenden, B M Hargis, G Tellez
Distillers dried grains with solubles (DDGS) have increasingly been used in poultry diets as a consequence of rising grain costs. Some, but not all, sources of DDGS have a variable compositional value, and a high inclusion of this by-product could be considered a risk factor for presentation of enteric diseases. Presently, 2 experiments were conducted using a starter corn-soybean diet (zero to 7 d) and a corn-DDGS-soybean grower diet (8 to 28 d) with or without inclusion of a Bacillus-direct-fed microbial (DFM)...
April 17, 2017: Poultry Science
https://www.readbyqxmd.com/read/28418713/defence-factors-of-vaginal-lactobacilli
#17
S Kovachev
Accurate knowledge of the composition and ecology of the vaginal microbial environment in healthy women is necessary for understanding lactobacilli-dominated normal microbiota and the mechanisms by which it reduces disease risk. The vagina and its microbiota form a balanced ecosystem; any movement outside the normal range for this ecosystem of obligate and/or facultative microbes, termed dysbacteriosis, can lead to infection and disease. This review summarizes recent research on the vaginal ecosystem, the Lactobacillus species that dominate it, and the means by which they suppress the growth, development, and/or proliferation of pathogenic microbial species...
April 18, 2017: Critical Reviews in Microbiology
https://www.readbyqxmd.com/read/28418108/metabolic-characterization-and-transformation-of-the-non-dairy-lactococcus-lactis-strain-kf147-for-production-of-ethanol-from-xylose
#18
Kia Vest Petersen, Jianming Liu, Jun Chen, Jan Martinussen, Peter Ruhdal Jensen, Christian Solem
The non-dairy lactic acid bacterium Lactococcus lactis KF147 can utilize xylose as the sole energy source. To assess whether KF147 could serve as a platform organism for converting second generation sugars into useful chemicals, we characterized growth and product formation for KF147 when grown on xylose. In a defined medium KF147 was found to co-metabolize xylose and arginine, resulting in bi-phasic growth. Especially at low xylose concentrations, arginine significantly improved growth rate. To facilitate further studies of the xylose metabolism, we eliminated arginine catabolism by deleting the arcA gene encoding the arginine deiminase...
April 18, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28416855/production-and-characterization-of-functional-properties-of-protein-hydrolysates-from-egg-shell-membranes-by-lactic-acid-bacteria-fermentation
#19
Surangna Jain, Anil Kumar Anal
This study aimed to ferment the chicken eggshell membrane (ESM) using the lactic acid bacteria, Lactobacillus plantarum for preparation of functional and bioactive protein hydrolysates. Cultivation at an initial pH of 8.0 for 36 h resulted in maximum protein concentration (177.3 mg/g) and degree of hydrolysis (25.1%) of the hydrolysates. Fermentation resulted in the production of hydrolysates that demonstrated excellent solubility (90.7%), good foaming capacity (36.7%) and emulsification activity (94.6 m(2)/g)...
April 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28414739/an-in-silico-pan-genomic-probe-for-the-molecular-traits-behind-lactobacillus-ruminis-gut-autochthony
#20
Ravi Kant, Airi Palva, Ingemar von Ossowski
As an ecological niche, the mammalian intestine provides the ideal habitat for a variety of bacterial microorganisms. Purportedly, some commensal genera and species offer a beneficial mix of metabolic, protective, and structural processes that help sustain the natural digestive health of the host. Among these sort of gut inhabitants is the Gram-positive lactic acid bacterium Lactobacillus ruminis, a strict anaerobe with both pili and flagella on its cell surface, but also known for being autochthonous (indigenous) to the intestinal environment...
2017: PloS One
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