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Lactic acid ldh

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https://www.readbyqxmd.com/read/28622146/relationship-between-various-maternal-conditions-and-lactic-acid-dehydrogenase-activity-in-umbilical-cord-blood-at-birth
#1
Evangelos Patavoukas, Josefina Åberg-Liesaho, Cecilia Pegelow Halvorsen, Birger Winbladh, Eva Wiberg-Itzel
BACKGROUND: Lactic acid dehydrogenase (LDH) is a valuable marker for some of the most important diseases in newborns and the plasma LDH activity in newborns correlates well with conditions such as asphyxia. If LDH should be considered as a useful tool also in obstetric care, key factors associated with maternal health before and during pregnancy which could affect umbilical cord LDH activity need to be known. The aims of this study were to explore relationships between selected maternal conditions and arterial lactic acid dehydrogenase activity (aLDH) in umbilical cord blood at delivery...
June 15, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28620491/pseudo-thrombotic-thrombocytopenic-purpura-presenting-as-multi-organ-dysfunction-syndrome-a-rare-complication-of-pernicious-anemia
#2
Saroj Kandel, Nibash Budhathoki, Shanta Pandey, Bikash Bhattarai, Aam Baqui, Ramesh Pandey, Divya Salhan, Danilo Enriquez, Joseph Quist, Frances M Schmidt
OBJECTIVE: We present a rare case of pernicious anemia presented as multi-organ dysfunction syndrome, later found to have pseudo-thrombotic thrombocytopenic purpura. METHODS: An 86-year-old female presented with respiratory distress, altered mental status, acute renal failure and was intubated in emergency room. She was found to have severe anemia, thrombocytopenia, high lactate, high lactate dehydrogenase and low haptoglobin. Peripheral smear revealed multilobulated neutrophils with schistocytes, poikilocytes and anisocytes...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28588747/antifatigue-effect-of-luteolin-6-c-neohesperidoside-on-oxidative-stress-injury-induced-by-forced-swimming-of-rats-through-modulation-of-nrf2-are-signaling-pathways
#3
Fang-Fang Duan, Ying Guo, Jing-Wan Li, Ke Yuan
Luteolin-6-C-neohesperidoside (LN) is a flavonoid isolated from moso bamboo leaf. This study was performed to evaluate the antifatigue effect of LN on a rat model undergoing the weight-loaded forced swimming test (FST). Briefly, male Sprague-Dawley rats (20-22 weeks old) were forced to undertake exhaustive swimming every other day for 3 weeks. Each swimming session was followed by the administration of distilled water, LN (25-75 mg/kg), or ascorbic acid (100 mg/kg) 1 h later. Oral administration of LN significantly improved exercise endurance; normalized alterations in energy metabolic markers; and decreased serum lactic acid, lactate dehydrogenase, and blood urea nitrogen levels of rats that underwent FST...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28588427/evaluation-of-anti-fatigue-property-of-the-extruded-product-of-cereal-grains-mixed-with-cordyceps-militaris-on-mice
#4
Lei Zhong, Liyan Zhao, Fangmei Yang, Wenjian Yang, Yong Sun, Qiuhui Hu
BACKGROUND: Fatigue is a biological phenomenon that involves a feeling of extreme physical or mental tiredness that could potentially cause some severe chronic diseases. Recently, diet therapy has provided a new alternative to alleviate physical fatigue. In our previous study, addition of Cordyceps militaris (C. militaris) into an extruded product was shown to provide high nutrition and unique flavors; however, little is known whether this product has some scientific evidence regarding anti-fatigue property...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28583672/monascus-ruber-as-cell-factory-for-lactic-acid-production-at-low-ph
#5
Ruud A Weusthuis, Astrid E Mars, Jan Springer, Emil Jh Wolbert, Hetty van der Wal, Truus G de Vrije, Mark Levisson, Audrey Leprince, G Bwee Houweling-Tan, Antoine Pha Moers, Sjon Na Hendriks, Odette Mendes, Yvonne Griekspoor, Marc Wt Werten, Peter J Schaap, John van der Oost, Gerrit Eggink
A Monascus ruber strain was isolated that was able to grow on mineral medium at high sugar concentrations and 175g/l lactic acid at pH 2.8. Its genome and transcriptomes were sequenced and annotated. Genes encoding lactate dehydrogenase (LDH) were introduced to accomplish lactic acid production and two genes encoding pyruvate decarboxylase (PDC) were knocked out to subdue ethanol formation. The strain preferred lactic acid to glucose as carbon source, which hampered glucose consumption and therefore also lactic acid production...
June 3, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28534828/how-ketamine-affects-livers-of-pregnant-mice-and-developing-mice
#6
Hoi Man Cheung, Tony Chin Hung Chow, David Tai Wai Yew
It is well known that ketamine abuse can induce liver damage in adult addicts, but the effects of ketamine abuse in pregnant mothers on their offspring have received less attention. In this study, we investigated the effects of 5-day ketamine injections (30 mg/kg) to pregnant Institute for Cancer Research (ICR) mice during early gestation or mid-gestation on the aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities of the mothers and the offspring. We also looked into whether administering ketamine treatment to the mothers had any effects on the extent of fibrosis, cell proliferation and cell death in the livers of the newborns...
May 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28524038/-intervention-effect-of-qingzao-runfei-huazhuo-xingxue-decoction-on-pm2-5-induced-pulmonary-injury-in-mice
#7
Jinbo Zhang, Lei Zhang, Yanxia Chen, Xinlu Wang, Aihua Hou, Lingling Dai
OBJECTIVE: To study the effects of Qingzao Runfei Huazhuo Xingxue decoction (QRHXD) on inflammatory reaction and histopathology in mice with PM2.5-induced pulmonary injury, and to approach the possible mechanism of prevention and treatment of traditional Chinese medicine on lung injury induced by haze. METHODS: Fifty healthy male C57BL/6 mice were randomly divided into five groups (n = 10): namely control, PM2.5, PM2.5 + low-, moderate-, and high-dose groups. The PM2...
May 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28502301/-psychological-distress-promotes-proliferation-and-invasion-of-glioma-by-upregulating-lactate-deoxygenase-a
#8
Ping Wang, Kunpeng Lyu, Qingluan Xu, Fengxiang Sun, Aixia Meng, Junhong Dong
Objective To investigate the role of lactate deoxygenase A (LDHA) expression in the proliferation and invasion of glioma promoted by the psychological distress. Methods The glioma-bearing nude mice were divided into tumor bearing group, stress tumor bearing group, negative control, short hairpin RNA (shRNA)-lactate dehydrogenase A (LDHA) group and shRNA-LDHA stress group. Four weeks after stress, the tumor size of each group was measured; meanwhile, norepinephrine (NE) andadrenaline (EPI) in the blood were detected by ELISA; lactic acid in the tumor tissue was determined by colorimetry...
May 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28484259/the-physiological-and-molecular-response-of-aurelia-sp-1-under-hypoxia
#9
Guoshan Wang, Yu Zhen, Zhigang Yu, Yan Shi, Qing Zhao, Jianyan Wang, Tiezhu Mi
Few studies have been published on the mechanisms of hypoxia response and tolerance in jellyfish, especially with respect to the regulatory mechanism at the molecular level. In this study, Aurelia sp.1, which is frequently found in Chinese coastal waters, was cultivated in a hypoxic system to determine the molecular mechanisms underlying its hypoxic response by studying the physiological activity, gene expression and metabolite contents in the prolyl hydroxylase domain (PHD)-hypoxia inducible factor (HIF) oxygen-sensing system...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28475210/enhanced-d-lactic-acid-production-by-recombinant-saccharomyces-cerevisiae-following-optimization-of-the-global-metabolic-pathway
#10
Ryosuke Yamada, Kazuki Wakita, Ryosuke Mitsui, Hiroyasu Ogino
Utilization of renewable feedstocks for the production of bio-based chemicals such as d-lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d-lactic acid production by S. cerevisiae, the expression of 12 glycolysis-related genes and the Leuconostoc mesenteroides d-LDH gene was optimized using a previously developed global metabolic engineering strategy, and repeated batch fermentation was carried out using the resultant strain YPH499/dPdA3-34/DLDH/1-18...
May 5, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28461153/a-coupled-photometric-assay-for-characterization-of-s-adenosyl-l-homocysteine-hydrolases-in-the-physiological-hydrolytic-direction
#11
Lyn L Kailing, Daniela Bertinetti, Friedrich W Herberg, Ioannis V Pavlidis
S-Adenosyl-L-homocysteine hydrolases (SAHases) are important metabolic enzymes and their dysregulation is associated with some severe diseases. In vivo they catalyze the hydrolysis of S-adenosyl-L-homocysteine (SAH), the by-product of methylation reactions in various organisms. SAH is a potent inhibitor of methyltransferases, thus its removal from the equilibrium is an important requirement for methylation reactions. SAH hydrolysis is also the first step in the cellular regeneration process of the methyl donor S-adenosyl-L-methionine (SAM)...
April 28, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28374137/the-proliferation-mechanism-of-lactobacillus-plantarum-rb1-stimulated-by-stachyose
#12
Qing Pan, Xiaoqun Zeng, Daodong Pan, Liuyang Peng, Zhen Wu, Yangying Sun, Yan Wei
The proliferation mechanism of Lactobacillus plantarum RB1 promoted by stachyose was investigated in this work. The hydrolysis of stachyose, the glycometabolism, and the cytoactivity of L. plantarum RB1 were detected after proliferation. The specific activity of α-galactosidase of L. plantarum RB1 in the stachyose group was significantly higher than the control group (without stachyose), which indicated that the stachyose induced L. plantarum RB1 and produced more α-galactosidase to hydrolyze stachyose. The glycometabolism which includes glycolysis and tricarboxylic acid (TCA) cycle was significantly enhanced in the stachyose group compared with the control group...
April 4, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28370718/m2-like-macrophage-polarization-in-high-lactic-acid-producing-head-and-neck-cancer
#13
Toshimitsu Ohashi, Mitsuhiro Aoki, Hiroyuki Tomita, Takashi Akazawa, Katsuya Sato, Bunya Kuze, Keisuke Mizuta, Akira Hara, Hitoshi Nagaoka, Norimitsu Inoue, Yatsuji Ito
Reprogramming of glucose metabolism in tumor cells is referred to as the Warburg effect and results in increased lactic acid secretion into the tumor microenvironment. We have previously shown that lactic acid has important roles as a pro-inflammatory and immunosuppressive mediator and promotes tumor progression. In this study, we examined the relationship between the lactic acid concentration and expression of LDHA and GLUT1, which are related to the Warburg effect, in human head and neck squamous cell carcinoma (HNSCC)...
April 1, 2017: Cancer Science
https://www.readbyqxmd.com/read/28314283/stable-shrna-silencing-of-lactate-dehydrogenase-a-ldha-in-human-mda-mb-231-breast-cancer-cells-fails-to-alter-lactic-acid-production-glycolytic-activity-atp-or-survival
#14
Nzinga Mack, Elizabeth A Mazzio, David Bauer, Hernan Flores-Rozas, Karam F A Soliman
BACKGROUND: In the US, African Americans have a high death rate from triple-negative breast cancer (TNBC), characterized by lack of hormone receptors (ER, PR, HER2/ERRB2) which are otherwise valuable targets of chemotherapy. There is a need to identify novel targets that negatively impact TNBC tumorigenesis. TNBCs release an abundance of lactic acid, under normoxic, hypoxic and hyperoxic conditions; this referred to as the Warburg effect. Accumulated lactic acid sustains peri-cellular acidity which propels metastatic invasion and malignant aggressive transformation...
March 2017: Anticancer Research
https://www.readbyqxmd.com/read/28231388/structural-characterization-and-antifatigue-effect-in-vivo-of-maca-lepidium-meyenii-walp-polysaccharide
#15
Weimin Tang, Lu Jin, Lianghua Xie, Juqing Huang, Nan Wang, Bingquan Chu, Xulin Dai, Yu Liu, Rui Wang, Ying Zhang
Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo. The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses. The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model. MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793...
March 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28198606/sustained-release-of-brimonidine-from-a-new-composite-drug-delivery-system-for-treatment-of-glaucoma
#16
Jianguo Sun, Yuan Lei, Zhaoxing Dai, Xi Liu, Taomin Huang, Jihong Wu, Zhi Ping Xu, Xinghuai Sun
A novel layered double hydroxide (LDH) nanoparticle/thermogel composite drug delivery system (DDS) for sustained release of brimonidine (Bri) has been designed, prepared, and characterized in this study for treatment of severe glaucoma. Brimonidine is first loaded onto LDH (Bri@LDH) nanoparticles, which are then dispersed in the thermogel consisting of plenty of micelles based on poly(dl-lactic acid-co-coglycolic acid)-polyethylene glycol-poly(dl-lactic acid-co-coglycolic acid) (PLGA-PEG-PLGA) copolymer. The Bri@LDH/Thermogel DDS containing 125...
February 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28159797/homolactic-acid-fermentation-by-the-genetically-engineered-thermophilic-homoacetogen-moorella-thermoacetica-atcc-39073
#17
Yuki Iwasaki, Akihisa Kita, Koichiro Yoshida, Takahisa Tajima, Shinichi Yano, Tomohiro Shou, Masahiro Saito, Junichi Kato, Katsuji Murakami, Yutaka Nakashimada
For the efficient production of target metabolites from carbohydrates, syngas, or H2-CO2 by genetically engineered Moorella thermoacetica, the control of acetate production (a main metabolite of M. thermoacetica) is desired. Although propanediol utilization protein (PduL) was predicted to be a phosphotransacetylase (PTA) involved in acetate production in M. thermoacetica, this has not been confirmed. Our findings described herein directly demonstrate that two putative PduL proteins, encoded by Moth_0864 (pduL1) and Moth_1181 (pduL2), are involved in acetate formation as PTAs...
April 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28106808/anti-fatigue-effects-of-the-unique-polysaccharide-marker-of-dendrobium-officinale-on-balb-c-mice
#18
Wei Wei, Zhi-Peng Li, Tong Zhu, Hau-Yee Fung, Tin-Long Wong, Xin Wen, Dik-Lung Ma, Chung-Hang Leung, Quan-Bin Han
Dendrobium officinale extract shows potent anti-fatigue effects; however, the active substance responsible for these effects remains undetermined. A glucomannan with a huge molecular size of 730 kDa, called DOP, was identified as the unique authentication marker of this expensive herb. DOP exhibited immunomodulating effects on macrophages and lymphocytes in our previous study. Clinical reports also showed that people with fatigue syndrome have a disturbed immune system. Because DOP is the unique and dominant component of D...
January 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28075415/inhibitory-effects-of-probiotic-lactobacillus-on-the-growth-of-human-colonic-carcinoma-cell-line-ht-29
#19
Zhung-Yuan Chen, You-Miin Hsieh, Chun-Chih Huang, Cheng-Chih Tsai
This study was conducted to investigate the inhibitory effect of Lactobacillus cells and supernatants on the growth of the human colon cancer cell line HT-29. Our study results indicated that the PM153 strain exhibits the best adhesion ability and the highest survival in the gastrointestinal tract simulation experiment. Furthermore, after an 8-h co-culture of PM153 and HT-29 cells, the PM153 strain can induce the secretion of nitric oxide from the HT-29 cells. In addition, after the co-culture of the BCRC17010 strain (10⁸ cfu/mL) and HT-29 cells, the Bax/Bcl-2 ratio in the HT-29 cells was 1...
January 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27989750/the-antioxidant-uncoupling-protein-2-stimulates-hnrnpa2-b1-glut1-and-pkm2-expression-and-sensitizes-pancreas-cancer-cells-to-glycolysis-inhibition
#20
Jessica Brandi, Daniela Cecconi, Marco Cordani, Margalida Torrens-Mas, Raffaella Pacchiana, Elisa Dalla Pozza, Giovanna Butera, Marcello Manfredi, Emilio Marengo, Jordi Oliver, Pilar Roca, Ilaria Dando, Massimo Donadelli
Several evidence indicate that metabolic alterations play a pivotal role in cancer development. Here, we report that the mitochondrial uncoupling protein 2 (UCP2) sustains the metabolic shift from mitochondrial oxidative phosphorylation (mtOXPHOS) to glycolysis in pancreas cancer cells. Indeed, we show that UCP2 sensitizes pancreas cancer cells to the treatment with the glycolytic inhibitor 2-deoxy-D-glucose. Through a bidimensional electrophoresis analysis, we identify 19 protein species differentially expressed after treatment with the UCP2 inhibitor genipin and, by bioinformatic analyses, we show that these proteins are mainly involved in metabolic processes...
December 2016: Free Radical Biology & Medicine
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