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Lactic acid ldh

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https://www.readbyqxmd.com/read/28198606/sustained-release-of-brimonidine-from-a-new-composite-drug-delivery-system-for-treatment-of-glaucoma
#1
Jianguo Sun, Yuan Lei, Zhaoxing Dai, Xi Liu, Taomin Huang, Jihong Wu, Zhi Ping Xu, Xinghuai Sun
A novel layered double hydroxide (LDH) nanoparticle/thermogel composite drug delivery system (DDS) for sustained release of brimonidine (Bri) has been designed, prepared and characterized in this study for treatment of severe glaucoma. Brimonidine is first loaded onto LDH (Bri@LDH) nanoparticles, which are then dispersed in the thermogel consisting of plenty of micelles based on poly-(DL-lactic acid co-glycolic acid)-polyethylene glycol-poly-(DL-lactic acid co-glycolic acid) (PLGA-PEG-PLGA) copolymer. The Bri@LDH/Thermogel DDS containing 125...
February 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28159797/homolactic-acid-fermentation-by-the-genetically-engineered-thermophilic-homoacetogen-moorella-thermoacetica-atcc39073
#2
Yuki Iwasaki, Akihisa Kita, Koichiro Yoshida, Takahisa Tajima, Shinichi Yano, Tomohiro Shou, Masahiro Saito, Junichi Kato, Katsuji Murakami, Yutaka Nakashimada
: For the efficient production of target metabolites from carbohydrates, syngas or H2-CO2 by genetically engineered Moorella thermoacetica, the control of acetate production (a main metabolite of M. thermoacetica) is desired. Although propanediol utilization protein (PduL) was predicted to be a phosphotransacetylase (PTA) involved in acetate production in M. thermoacetica, this has not been confirmed. Our findings described herein directly demonstrate that two putative PduL proteins, encoded by Moth_0864 (pduL1) and Moth_1181 (pduL2), respectively, are involved in acetate formation as PTAs...
February 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28106808/anti-fatigue-effects-of-the-unique-polysaccharide-marker-of-dendrobium-officinale-on-balb-c-mice
#3
Wei Wei, Zhi-Peng Li, Tong Zhu, Hau-Yee Fung, Tin-Long Wong, Xin Wen, Dik-Lung Ma, Chung-Hang Leung, Quan-Bin Han
Dendrobium officinale extract shows potent anti-fatigue effects; however, the active substance responsible for these effects remains undetermined. A glucomannan with a huge molecular size of 730 kDa, called DOP, was identified as the unique authentication marker of this expensive herb. DOP exhibited immunomodulating effects on macrophages and lymphocytes in our previous study. Clinical reports also showed that people with fatigue syndrome have a disturbed immune system. Because DOP is the unique and dominant component of D...
January 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28075415/inhibitory-effects-of-probiotic-lactobacillus-on-the-growth-of-human-colonic-carcinoma-cell-line-ht-29
#4
Zhung-Yuan Chen, You-Miin Hsieh, Chun-Chih Huang, Cheng-Chih Tsai
This study was conducted to investigate the inhibitory effect of Lactobacillus cells and supernatants on the growth of the human colon cancer cell line HT-29. Our study results indicated that the PM153 strain exhibits the best adhesion ability and the highest survival in the gastrointestinal tract simulation experiment. Furthermore, after an 8-h co-culture of PM153 and HT-29 cells, the PM153 strain can induce the secretion of nitric oxide from the HT-29 cells. In addition, after the co-culture of the BCRC17010 strain (10⁸ cfu/mL) and HT-29 cells, the Bax/Bcl-2 ratio in the HT-29 cells was 1...
January 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27989750/the-antioxidant-uncoupling-protein-2-stimulates-hnrnpa2-b1-glut1-and-pkm2-expression-and-sensitizes-pancreas-cancer-cells-to-glycolysis-inhibition
#5
Jessica Brandi, Daniela Cecconi, Marco Cordani, Margalida Torrens-Mas, Raffaella Pacchiana, Elisa Dalla Pozza, Giovanna Butera, Marcello Manfredi, Emilio Marengo, Jordi Oliver, Pilar Roca, Ilaria Dando, Massimo Donadelli
Several evidence indicate that metabolic alterations play a pivotal role in cancer development. Here, we report that the mitochondrial uncoupling protein 2 (UCP2) sustains the metabolic shift from mitochondrial oxidative phosphorylation (mtOXPHOS) to glycolysis in pancreas cancer cells. Indeed, we show that UCP2 sensitizes pancreas cancer cells to the treatment with the glycolytic inhibitor 2-deoxy-D-glucose. Through a bidimensional electrophoresis analysis, we identify 19 protein species differentially expressed after treatment with the UCP2 inhibitor genipin and, by bioinformatic analyses, we show that these proteins are mainly involved in metabolic processes...
October 27, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/27983571/anti-fatigue-effects-of-small-molecule-oligopeptides-isolated-from-panax-ginseng-c-a-meyer-in-mice
#6
Lei Bao, Xiaxia Cai, Junbo Wang, Yuan Zhang, Bin Sun, Yong Li
Panax ginseng C. A. Meyer (ginseng) is an edible and medicinal Chinese herb, which is often used in Asian countries for physical fitness. Ginseng is reported to have a wide range of biological activity and pharmaceutical properties. There were more studies on ginsenosides and polysaccharides, but fewer studies on ginseng oligopeptides (GOP), which are small molecule oligopeptides isolated from ginseng. The present study was designed to evaluate the anti-fatigue effects of GOP in mice and explore the possible underlying mechanism...
December 13, 2016: Nutrients
https://www.readbyqxmd.com/read/27885267/contributory-roles-of-two-l-lactate-dehydrogenases-for-l-lactic-acid-production-in-thermotolerant-bacillus-coagulans
#7
Lifan Sun, Caili Zhang, Pengcheng Lyu, Yanping Wang, Limin Wang, Bo Yu
Thermotolerant Bacillus coagulans is considered to be a more promising producer for bio-chemicals, due to its capacity to withstand harsh conditions. Two L-lactate dehydrogenase (LDH) encoding genes (ldhL1 and ldhL2) and one D-LDH encoding gene (ldhD) were annotated from the B. coagulans DSM1 genome. Transcriptional analysis revealed that the expression of ldhL2 was undetectable while the ldhL1 transcription level was much higher than that of ldhD at all growth phases. Deletion of the ldhL2 gene revealed no difference in fermentation profile compared to the wild-type strain, while ldhL1 single deletion or ldhL1ldhL2 double deletion completely blocked L-lactic acid production...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27867078/co-expression-of-two-heterologous-lactate-dehydrogenases-genes-in-kluyveromyces-marxianus-for-l-lactic-acid-production
#8
Jae Won Lee, Jung Hoon In, Joon-Bum Park, Jonghyeok Shin, Jin Hwan Park, Bong Hyun Sung, Jung-Hoon Sohn, Jin-Ho Seo, Jin-Byoung Park, Soo Rin Kim, Dae-Hyuk Kweon
Lactic acid (LA) is a versatile compound used in the food, pharmaceutical, textile, leather, and chemical industries. Biological production of LA is possible by yeast strains expressing a bacterial gene encoding l-lactate dehydrogenase (LDH). Kluyveromyces marxianus is an emerging non-conventional yeast with various phenotypes of industrial interest. However, it has not been extensively studied for LA production. In this study, K. marxianus was engineered to express and co-express various heterologous LDH enzymes that were reported to have different pH optimums...
January 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/27852252/a-novel-pyruvate-kinase-and-its-application-in-lactic-acid-production-under-oxygen-deprivation-in-corynebacterium-glutamicum
#9
Xin Chai, Xiuling Shang, Yu Zhang, Shuwen Liu, Yong Liang, Yun Zhang, Tingyi Wen
BACKGROUND: Pyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involved in the tricarboxylic acid cycle, anaplerosis and energy anabolism, which places Pyk at a primary metabolic intersection. Pyks are highly conserved in most bacteria and lower eukaryotes. Corynebacterium glutamicum is an industrial workhorse for the production of various amino acids and organic acids...
November 16, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27834150/intranasal-delivery-of-microspheres-loaded-with-20-r-ginsenoside-rg3-enhances-anti-fatigue-effect-in-mice
#10
Wei Zhang, Xiaoyu Wang, Min Zhang, Min Xu, Wenyan Tang, Yi Zhang, Lei Lu, Jing Gao, Shen Gao
Nasal delivery of 20 (R) -ginsenoside Rg3 (Rg3) has a short-lived anti-fatigue effect owing to rapid clearance by nasal cilia. Thus, in order to extend the residence time of Rg3 in the nasal cavity, a new drug delivery system is needed. Chitosan microspheres loaded with Rg3 were prepared using a multi-step emulsification method. They had a spherical shape with a mean diameter of 44.9±12.6 µm. The drug-loading ratio was 10.25±0.08%, and the encapsulation ratio was 30.61±1.46%. Our in vitro mucoadhesion experiment demonstrated that 70...
November 9, 2016: Current Drug Delivery
https://www.readbyqxmd.com/read/27832309/production-of-optically-pure-d-lactic-acid-from-brown-rice-using-metabolically-engineered-lactobacillus-plantarum
#11
Kenji Okano, Shinji Hama, Maki Kihara, Hideo Noda, Tsutomu Tanaka, Akihiko Kondo
Simultaneous saccharification and fermentation (SSF) of D-lactic acid was performed using brown rice as both a substrate and a nutrient source. An engineered Lactobacillus plantarum NCIMB 8826 strain, in which the ʟ-lactate dehydrogenase gene was disrupted, produced 97.7 g/L D-lactic acid from 20% (w/v) brown rice without any nutrient supplementation. However, a significant amount of glucose remained unconsumed and the yield of lactic acid was as low as 0.75 (g/g-glucose contained in brown rice). Interestingly, the glucose consumption was significantly improved by adapting L...
November 10, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27812097/investigation-of-factors-affecting-aerobic-and-respiratory-growth-in-the-oxygen-tolerant-strain-lactobacillus-casei-n87
#12
Rocco G Ianniello, Teresa Zotta, Attilio Matera, Francesco Genovese, Eugenio Parente, Annamaria Ricciardi
Aerobic and respiratory cultivations provide benefits for some lactic acid bacteria (LAB). Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch cultivations with different dissolved oxygen (DO) concentrations. The expression of pox (pyruvate oxidase) and cydABCD operon (cytochrome bd oxidase complex) was quantified by quantitative Real Time polymerase chain reaction...
2016: PloS One
https://www.readbyqxmd.com/read/27802040/synthesis-of-layered-double-hydroxide-single-layer-nanosheets-in-formamide
#13
Jingfang Yu, Jingjing Liu, Abraham Clearfield, Johnathan E Sims, Michael T Speiegle, Steven L Suib, Luyi Sun
Layered double hydroxide (LDH) single-layer nanosheets were synthesized through a single-step process in the presence of formamide. This one-step process is simple, fast, and efficient and thus is potentially viable for large-scale production. Two key factors for the growth of LDH single-layer nanosheets, formamide concentration and LDH layer charge, were investigated thoroughly. A higher formamide concentration and a higher LDH layer charge are favorable for the growth of LDH single-layer nanosheets. The LDH single-layer nanosheets obtained at the premium formamide concentration and LDH layer charge were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and atomic force micrscopy (AFM)...
November 21, 2016: Inorganic Chemistry
https://www.readbyqxmd.com/read/27783320/lignans-rich-extract-from-herpetospermum-caudigerum-alleviate-physical-fatigue-in-mice
#14
Shi-Ying Jin, Rui-Sheng Li, Bao-de Shen, Jin-Xia Bai, Ping-Hua Xu, Ling Dai, He Xu, Shi-Xiao Jin, Jin Han, Hai-Long Yuan
OBJECTIVE: To ascertain anti-fatigue constituents and mechanisms of Herpetospermum caudigerum. METHODS: The 80% ethanol extracts of Herpetospermum caudigerum were partitioned with chloroform, ethyl acetate and n-butanol, respectively. Male Kunming mice were divided into 13 groups with 16 mice in each group: a control group fed with water, 9 groups treated with 3 fractions of Herpetospermum caudigerum (chloroform fraction, ethyl acetate fraction and n-butanol fraction) at dose of 80, 160 and 320 mg/kg for the low-dose group, medium-dose group and high-dose group, 3 herpetrione (HPE) treated groups fed with HPE at dose of 15, 30, and 60 mg/kg for the low-dose group, medium-dose group and high-dose group...
November 2016: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/27746306/direct-lactic-acid-production-from-beech-wood-by-transgenic-white-rot-fungus-phanerochaete-sordida-yk-624
#15
Toshio Mori, Hiroko Kako, Tomoki Sumiya, Hirokazu Kawagishi, Hirofumi Hirai
A lactic acid (LA)-producing strain of the hyper-lignin-degrading fungus Phanerochaete sordida YK-624 with the lactate dehydrogenase-encoding gene from Bifidobacterium longum (Blldh) was constructed. When the endogenous pyruvate decarboxylase gene-knocked down and Blldh-expressing transformant was cultured with beech wood meal, the transformant was able to successively delignify and ferment the substrate. Supplementation of calcium carbonate into the culture medium, significantly increased the level of LA accumulation...
December 10, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27723270/theranostic-bioabsorbable-bone-fixation-plate-with-drug-layered-double-hydroxide-nanohybrids
#16
Myung Hun Kim, Woojune Hur, Goeun Choi, Hye Sook Min, Tae Hyun Choi, Young Bin Choy, Jin-Ho Choy
A bioabsorbable polymeric bone plate enabled with both diagnostic and therapeutic functionalities (radiopacity and sustained drug release, respectively) is proposed. To this end, a drug-inorganic nanohybrid (RS-LDH) is examined as a theranostic agent by intercalating an anti-resorptive bone remodeling drug, risedronate (RS) into a layered double hydroxide (LDH) via an ion-exchange reaction. The RS-LDH is prepared as a sheet with a biodegradable polymer, poly(lactic-co-glycolic acid), and is then attached onto the clinically approved bioabsorbable bone plate to produce the theranostic plate...
November 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27641098/ldha-associated-lactic-acid-production-blunts-tumor-immunosurveillance-by-t-and-nk-cells
#17
Almut Brand, Katrin Singer, Gudrun E Koehl, Marlene Kolitzus, Gabriele Schoenhammer, Annette Thiel, Carina Matos, Christina Bruss, Sebastian Klobuch, Katrin Peter, Michael Kastenberger, Christian Bogdan, Ulrike Schleicher, Andreas Mackensen, Evelyn Ullrich, Stefan Fichtner-Feigl, Rebecca Kesselring, Matthias Mack, Uwe Ritter, Maximilian Schmid, Christian Blank, Katja Dettmer, Peter J Oefner, Petra Hoffmann, Stefan Walenta, Edward K Geissler, Jacques Pouyssegur, Andreas Villunger, André Steven, Barbara Seliger, Stephan Schreml, Sebastian Haferkamp, Elisabeth Kohl, Sigrid Karrer, Mark Berneburg, Wolfgang Herr, Wolfgang Mueller-Klieser, Kathrin Renner, Marina Kreutz
Elevated lactate dehydrogenase A (LDHA) expression is associated with poor outcome in tumor patients. Here we show that LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and NK cells. In immunocompetent C57BL/6 mice, tumors with reduced lactic acid production (Ldha(low)) developed significantly slower than control tumors and showed increased infiltration with IFN-γ-producing T and NK cells. However, in Rag2(-/-)γc(-/-) mice, lacking lymphocytes and NK cells, and in Ifng(-/-) mice, Ldha(low) and control cells formed tumors at similar rates...
September 7, 2016: Cell Metabolism
https://www.readbyqxmd.com/read/27634467/novel-homologous-lactate-transporter-improves-l-lactic-acid-production-from-glycerol-in-recombinant-strains-of-pichia-pastoris
#18
Pollyne Borborema Almeida de Lima, Kelly Cristina Leite Mulder, Nadiele Tamires Moreira Melo, Lucas Silva Carvalho, Gisele Soares Menino, Eduardo Mulinari, Virgilio H de Castro, Thaila F Dos Reis, Gustavo Henrique Goldman, Beatriz Simas Magalhães, Nádia Skorupa Parachin
BACKGROUND: Crude glycerol is the main byproduct of the biodiesel industry. Although it can have different applications, its purification is costly. Therefore, in this study a biotechnological route has been proposed for further utilization of crude glycerol in the fermentative production of lactic acid. This acid is largely utilized in food, pharmaceutical, textile, and chemical industries, making it the hydroxycarboxylic acid with the highest market potential worldwide. Currently, industrial production of lactic acid is done mainly using sugar as the substrate...
September 15, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27602272/novel-fermented-chickpea-milk-with-enhanced-level-of-%C3%AE-aminobutyric-acid-and-neuroprotective-effect-on-pc12-cells
#19
Wen Li, Mingming Wei, Junjun Wu, Xin Rui, Mingsheng Dong
In this study, novel fermented chickpea milk with high γ -aminobutyric acid (GABA) content and potential neuroprotective activity was developed. Fermentation starter that can produce GABA was selected from 377 strains of lactic acid bacteria isolated from traditional Chinese fermented foods. Among the screened strains, strain M-6 showed the highest GABA-producing capacity in De Man-Rogosa and Sharp (MRS) broth and chickpea milk. M-6 was identified as Lactobacillus plantarum based on Gram staining, API carbohydrate fermentation pattern testing, and 16s rDNA sequencing...
2016: PeerJ
https://www.readbyqxmd.com/read/27586425/salvianolic-acid-b-induced-upregulation-of-mir-30a-protects-cardiac-myocytes-from-ischemia-reperfusion-injury
#20
Dan Li, Jun Wang, Jincai Hou, Jianhua Fu, Jianxun Liu, Ruichao Lin
BACKGROUND: MicroRNAs (miRNAs) are a novel class of powerful, endogenous regulators of gene expression. This study was designed to ascertain if miR-30a is involved in the cardioprotective actions of salvianolic acid B (Sal B) against myocardial ischemia-reperfusion (I-R) injury through suppression of autophagy. METHODS: Murine myocardial cells that had undergone primary culture were induced by I-R and incubated with Sal B (25, 50, 100 μM) in the presence of a miR-30a mimic or miR-30a inhibitor...
September 1, 2016: BMC Complementary and Alternative Medicine
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