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https://www.readbyqxmd.com/read/28089928/improved-catalytic-properties-of-penicillium-notatum-lipase-immobilized-in-nanoscale-silicone-polymeric-films
#1
Saima Rehman, Ping Wang, Haq Nawaz Bhatti, Muhammad Bilal, Muhammad Asgher
Lipases are one of the most proficient biocatalysts having enormous biotechnological prospective. Immobilization offers a potential solution to improve the stability and recycling characteristics of lipases. An extracellular lipase from Penicillium notatum (PNL) was immobilized in silicon polymers (SiP) through entrapment, and subsequently coated this matrix on the network of fibers in the sponges. The silicone polymers-immobilized lipase (SiP-lipase) displayed highest apparent activity and entrapment efficiency of 1...
January 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28072605/oxytocin-alleviates-orofacial-mechanical-hypersensitivity-associated-with-infraorbital-nerve-injury-via-vasopressin-1a-receptors-of-the-rat-trigeminal-ganglia
#2
Asako Kubo, Masamichi Shinoda, Ayano Katagiri, Mamoru Takeda, Tatsuro Suzuki, Junichi Asaka, David C Yeomans, Koichi Iwata
Oxytocin (OXT) is a neuropeptide hormone synthesized and secreted by hypothalamic neurons, and has been reported to play a significant role in pain modulation. However, the mechanisms underlying OXT's antinociceptive effect on neuropathic pain are not fully understood. In the present study, we examined the peripheral effect of OXT on mechanical hypersensitivity induced by partial ligation of the infraorbital nerve (PNL) in rats. Mechanical hypersensitivity in the whisker pad skin following PNL was attenuated by the direct administration of OXT into the trigeminal ganglion (TG)...
January 7, 2017: Pain
https://www.readbyqxmd.com/read/28013344/residual-stones-after-percutaneous-nephrolithotomy-comparison-of-intraoperative-assessment-and-postoperative-non-contrast-computerized-tomography
#3
Ahmed M Harraz, Yasser Osman, Ahmed R El-Nahas, Amr A Elsawy, Islam Fakhreldin, Osama Mahmoud, Ahmed El-Assmy, Ahmed A Shokeir
OBJECTIVES: To compare the intraoperative surgeon perspective for detection of residual fragments (RFs) after percutaneous nephrolithotomy (PNL) with postoperative NCCT. METHODS: A prospective study of adult patients who underwent PNL between March and September 2014 was conducted. Stone complexity was evaluated using the Guy's stone score (GSS). All patients were evaluated by pre- and postoperative NCCT. After the procedure, the surgeon had been asked whether there were residual stones or not...
December 24, 2016: World Journal of Urology
https://www.readbyqxmd.com/read/27986574/mammography-before-and-after-bariatric-surgery
#4
Tara E Mokhtari, Ulysses S Rosas, John R Downey, Kanae K Miyake, Debra M Ikeda, John M Morton
BACKGROUND: Morbidly obese women are at increased risk for breast cancer, and the majority of surgical weight-loss patients are older than 40 years old. OBJECTIVE: The purpose of the present study was to determine the technical and interpretive changes in mammography following bariatric surgery. SETTING: Accredited Academic Hospital. METHODS: Two breast-imaging radiologists reviewed screening mammograms performed on 10 morbidly obese women undergoing bariatric surgery both pre- and postoperatively...
October 31, 2016: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
https://www.readbyqxmd.com/read/27973459/percutaneous-nephrolithotomy-with-amplatz-and-alken-dilators-an-eight-year-single-tertiary-care-centre-experience
#5
Piotr Bryniarski, Paweł Stelmach, Piotr Taborowski, Paweł Rajwa, Mateusz Adamkiewicz, Marcin Życzkowski, Andrzej Paradysz
BACKGROUND Percutaneous nephrolithotomy (PNL) is the standard procedure for patients with renal stones over 2 cm in diameter. We analyzed complications after this procedure focusing on two different methods of tract dilation. MATERIAL AND METHODS Between August 2008 and April 2016 222 percutaneous nephrolithotomies were performed in a total of 208 patients. The Group I (n=123) comprised patients where Alken dilatators were used, while Group II (n=99) comprised patients where Amplatz dilators were used. Efficacy was examined based on ultrasound and x-ray examination one month after the procedure...
December 14, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27845226/enhancement-of-solubility-purification-and-inclusion-bodies-refolding-of-an-active-pectin-lyase-from-penicillium-occitanis-expressed-in-escherichia-coli
#6
Azza Hadj Sassi, Hèla Trigui-Lahiani, Salma Abdeljalil, Ali Gargouri
Pectin lyase (pnl) is the only pectinase able to hydrolyze directly the highly methylated pectin without liberating the toxic methanol and without disturbing ester content responsible for specific aroma of juices. The cDNA of Penicillium occitanis pnl (mature form) was cloned into pET-21a as expression vector and over-expressed into Esherichia coli. Most of recombinant pnl was expressed as inclusion bodies. Pnl activity was confirmed by colorimetric assay. To enhance the solubility yield of the expressed pnl, the effects of induction temperature, host strain and expression level were optimized...
February 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27822940/national-practice-pattern-and-time-trends-in-treatment-of-upper-urinary-tract-calculi-in-korea-a-nationwide-population-based-study
#7
Jinsung Park, Beomseok Suh, Myung Shin Lee, Seung Hyo Woo, Dong Wook Shin
Despite high prevalence of upper urinary tract calculi (UUTC), there are few studies regarding patterns of care in Asian populations. We investigated treatment patterns and time trends in patients with newly diagnosed UUTC in Korea using the National Health Insurance database that includes de-identified claims from a random 2% sample of the entire population (> 1 million people). A total of 14,282 patients who received active treatments, including shock wave lithotripsy (SWL), ureteroscopic surgery (URS), percutaneous nephrolithotomy (PNL), and uretero/pyelolithotomy (UPL), for newly diagnosed UUTC between 2003 and 2013 were included...
December 2016: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/27784164/re-abbreviation-of-percutaneous-nephrolithotomy-pcnl-or-pnl-from-haluk-s%C3%A3-ylemez-j-endourol-30-7-830
#8
Christian Türk
No abstract text is available yet for this article.
November 29, 2016: Journal of Endourology
https://www.readbyqxmd.com/read/27738537/diffusion-tensor-imaging-tractography-in-pure-neuritic-leprosy-first-experience-report-and-review-of-the-literature
#9
Michele R Colonna, Giuseppe Tallarida, Francesco Stagno d'Alcontres, Salvatore Noto, Aurora Parodi, Alberto Tagliafico
Five years after both right ulnar and median nerve decompression for paraesthesias and palsy, a patient, coming from Nigeria but living in Italy, came to our unit claiming to have persistent pain and combined median and ulnar palsy. Under suspicion of leprosy, skin and left sural nerve biopsy were performed. Skin tests were negative, but Schwann cells resulted as positive for acid-fast bacilli (AFB), leading to the diagnosis of Pure Neuritic Leprosy (PNL). The patient was given PB multidrug therapy and recovered from pain in two months...
2016: Case Reports in Neurological Medicine
https://www.readbyqxmd.com/read/27704184/mini-percutaneous-nephrolithotomy-for-stones-in-anomalous-kidneys-a-prospective-study
#10
Sanjay Khadgi, Babu Shrestha, Hamdy Ibrahim, Sunil Shrestha, Mohammed S ElSheemy, Ahmed M Al-Kandari
To evaluate safety and efficacy of minipercutaneous nephrolithotomy (Mini-PNL) in management of stones in different types of renal anomalies. Patients with stones ≥2 cm or SWL-resistant stones in anomalous-kidneys treated by Mini-PNL between March 2010 and September 2012 were included prospectively. Mini-PNL was done under regional anesthesia in prone position with fluoroscopic guidance through 18 Fr sheath using semirigid ureteroscope (8.5/11.5 Fr) and pneumatic lithotripter. All patients were followed-up for 2-3 years...
October 4, 2016: Urolithiasis
https://www.readbyqxmd.com/read/27635291/comparison-of-intraoperative-and-postoperative-complications-based-on-asa-risks-in-patients-who-underwent-percutaneous-nephrolithotomy
#11
Hüseyin Buğra Karakaş, İzzet Çiçekbilek, Adem Tok, Tamer Alışkan, Bülent Akduman
OBJECTIVE: In this study we aimed to evaluate intraoperative and postoperative complications which developed according to pre-operative American Society of Anesthesiologists (ASA) risk criteria in patients who had undergone percutaneous nephrolithotomy (PNL). MATERIAL AND METHODS: Five hundred and sixty patients who had undergone PNL between 2002 and 2014 were included in the study. Patients operated on the ipsilateral kidney, those with solitary kidney or the cases who had previously undergone more than one access were excluded from this study...
September 2016: Turkish Journal of Urology
https://www.readbyqxmd.com/read/27635289/combined-micro-and-standard-percutaneous-nephrolithotomy-for-complex-renal-calculi
#12
İbrahim Buldu, Abdulkadir Tepeler, Tuna Karatağ, Ramazan İnan, Abdullah Armağan, Okan İstanbulluoğlu
OBJECTIVE: We aimed to present the technique of combination of standard percutaneous nephrolithotomy (PNL) with microperc for achieving higher success rates without increasing complication rates in the management of complex renal calculi. MATERIAL AND METHODS: The patients who underwent microperc procedure as a complementary procedure to standard PNL for complex kidney stones in two reference hospitals between 2013 and 2015, were evaluated retrospectively. RESULTS: All patients underwent a total of two accesses one for standard PNL and one for microperc...
September 2016: Turkish Journal of Urology
https://www.readbyqxmd.com/read/27620184/-stone-treatment-tomorrow-and-the-day-after
#13
A Miernik, S Hein, F Adams, J Halbritter, M Schoenthaler
Urological and surgical treatment of urinary stones are highly technological and technology-driven disciplines in present-day surgery. German medical engineering has always been recognized for its technical innovations in endoscopic surgery. Current and future trends are indicative of further miniaturization and automation of surgical instruments and assist systems to facilitate endourological procedures as well as improvements in the quality of results and ergonomics. These technologies include, e. g. 3D-tracking to facilitate access to the pelvicaliceal system for percutaneous nephrolithotomy (PNL) or robotic master-slave systems for endourology...
October 2016: Der Urologe. Ausg. A
https://www.readbyqxmd.com/read/27617316/clinical-factors-associated-with-postoperative-hydronephrosis-after-ureteroscopic-lithotripsy
#14
Sun Woo Kim, Ji Hoon Ahn, Sang Un Yim, Yang Hyun Cho, Bo Sung Shin, Ho Seok Chung, Eu Chang Hwang, Ho Song Yu, Kyung Jin Oh, Sun-Ouck Kim, Seung Il Jung, Taek Won Kang, Dong Deuk Kwon, Kwangsung Park
PURPOSE: This study aimed to determine the predictors of ipsilateral hydronephrosis after ureteroscopic lithotripsy for ureteral calculi. MATERIALS AND METHODS: From January 2010 to December 2014, a total of 204 patients with ureteral calculi who underwent ureteroscopic lithotripsy were reviewed. Patients with lack of clinical data, presence of ureteral rupture, and who underwent simultaneous percutaneous nephrolithotomy (PNL) were excluded. Postoperative hydronephrosis was determined via computed tomographic scan or renal ultrasonography, at 6 months after ureteroscopic lithotripsy...
September 2016: Investigative and Clinical Urology
https://www.readbyqxmd.com/read/27551558/a-novel-dosimeter-for-measuring-the-amount-of-radiation-exposure-of-surgeons-during-percutaneous-nephrolithotomy-instadose%C3%A2
#15
Emrah Yuruk, Gokhan Gureser, Murat Tuken, Kasim Ertas, Ege Can Serefoglu
INTRODUCTION: The aim of this study was to demonstrate the efficacy of Instadose™, a novel dosimeter designed for radiation workers to provide a measurement of the radiation dose at any time from any computer; to determine the amount of radiation exposure during percutaneous nephrolithotomy (PNL); and to evaluate the factors that affect the amount of radiation exposed. MATERIAL AND METHODS: Two experienced surgeons wore Instadose™ on the outer part of their lead aprons during the PNL procedures performed between December 2013 and July 2014...
2016: Central European Journal of Urology
https://www.readbyqxmd.com/read/27551557/day-care-pnl-using-santosh-pgi-hemostatic-seal-versus-standard-pnl-a-randomized-controlled-study
#16
Santosh Kumar, Shivanshu Singh, Prashant Singh, Shrawan Kumar Singh
INTRODUCTION: To compare the outcomes of tubeless day care PNL using hemostatic seal in the access tract versus standard PNL. MATERIAL AND METHODS: It was a prospective randomized controlled study. Cases were randomized to either the day care group with hemostatic seal (DCS) or the control group where patients were admitted and a nephrostomy tube was placed at the conclusion of surgery. RESULTS: A total of 180 cases were screened and out of these, 113 were included in the final analysis...
2016: Central European Journal of Urology
https://www.readbyqxmd.com/read/27544374/dietary-zinc-deficiency-predisposes-mice-to-the-development-of-preneoplastic-lesions-in-chemically-induced-hepatocarcinogenesis
#17
Guilherme Ribeiro Romualdo, Renata Leme Goto, Ana Angélica Henrique Fernandes, Bruno Cogliati, Luis Fernando Barbisan
Although there is a concomitance of zinc deficiency and high incidence/mortality for hepatocellular carcinoma in certain human populations, there are no experimental studies investigating the modifying effects of zinc on hepatocarcinogenesis. Thus, we evaluated whether dietary zinc deficiency or supplementation alter the development of hepatocellular preneoplastic lesions (PNL). Therefore, neonatal male Balb/C mice were submitted to a diethylnitrosamine/2-acetylaminefluorene-induced hepatocarcinogenesis model...
October 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27504246/efficacy-and-safety-of-mini-percutaneous-nephrolithotomy-in-obese-patients
#18
Fatih Akbulut, Onur Kucuktopcu, Emre Kandemir, Burak Ucpinar, Faruk Ozgor, Abdulmuttalip Simsek, Burak Arslan, Akif Erbin, Fatih Yanaral, Murat Binbay, Gokhan Gurbuz
PURPOSE: We aimed to evaluate the effect of obesity on surgical outcomes of mini percutaneous nephrolithotomy (Mini-PNL). METHODS: Hundred and eighty two Mini-PNL procedures were performed between May 2013 and January 2015 and their results were evaluated retrospectively. Patients were classified as non-obese (BMI, 18.5-30 kg/m(2)) and obese (≥30 kg/m(2)) groups. Obese and non-obese patients were compared according to pre-operative demographic values, intra-operative surgery techniques and post-operative results...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27496295/percutaneous-nephrolithotomy-increases-the-risk-of-new-onset-hypertension-a-nationwide-6-year-follow-up-study
#19
Tsu-Ming Chien, Yen-Man Lu, Yii-Her Chou, Wen-Jeng Wu, Chun-Nung Huang
OBJECTIVE: To determine whether percutaneous nephrolithotomy or ureteroscopic lithotripsy leads to the development of hypertension, using the Taiwan National Health Insurance database. METHODS: Data were sourced from the Longitudinal Health Insurance Database (LHID2000) of Taiwan, Republic of China, compiled by the Taiwan National Health Insurance database from 1996 to 2010. Percutaneous nephrolithotomy and ureteroscopic lithotripsy were studied as time-dependent covariates in a Cox proportional hazard model to estimate the hazard ratio for the effect of new-onset hypertension...
November 2016: Urology
https://www.readbyqxmd.com/read/27473257/percutaneous-nephrolithotomy-in-children-with-pediatric-and-adult-sized-instruments
#20
Huseyin Celik, Ahmet Camtosun, Ramazan Altintas, Cemal Tasdemir
OBJECTIVE: Pediatric stone disease is a significant health issue which has increased in incidence because of lifestyle changes, dietary habits, and obesity. The incidence of urolithiasis among pediatric age groups varies according to region and is high in Turkey. The management of stone disease in children has improved dramatically over the last two decades. The high success rate of percutaneous nephrolithotomy (PNL) have led modern researchers to suggest that it be used as a first-line treatment of kidney stones greater than 2 cm in size...
December 2016: Journal of Pediatric Urology
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