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Azza Hadj Sassi, Hèla Trigui-Lahiani, Salma Abdeljalil, Ali Gargouri
Pectin lyase (pnl) is the only pectinase able to hydrolyze directly the highly methylated pectin without liberating the toxic methanol and without disturbing ester content responsible for specific aroma of juices. The cDNA of Penicillium occitanis pnl (mature form) was cloned into pET-21a as expression vector and over-expressed into Esherichia coli. Most of recombinant pnl was expressed as inclusion bodies. Pnl activity was confirmed by colorimetric assay. To enhance the solubility yield of the expressed pnl, the effects of induction temperature, host strain and expression level were optimized...
November 11, 2016: International Journal of Biological Macromolecules
Jinsung Park, Beomseok Suh, Myung Shin Lee, Seung Hyo Woo, Dong Wook Shin
Despite high prevalence of upper urinary tract calculi (UUTC), there are few studies regarding patterns of care in Asian populations. We investigated treatment patterns and time trends in patients with newly diagnosed UUTC in Korea using the National Health Insurance database that includes de-identified claims from a random 2% sample of the entire population (> 1 million people). A total of 14,282 patients who received active treatments, including shock wave lithotripsy (SWL), ureteroscopic surgery (URS), percutaneous nephrolithotomy (PNL), and uretero/pyelolithotomy (UPL), for newly diagnosed UUTC between 2003 and 2013 were included...
December 2016: Journal of Korean Medical Science
Christian Türk
No abstract text is available yet for this article.
November 29, 2016: Journal of Endourology
Michele R Colonna, Giuseppe Tallarida, Francesco Stagno d'Alcontres, Salvatore Noto, Aurora Parodi, Alberto Tagliafico
Five years after both right ulnar and median nerve decompression for paraesthesias and palsy, a patient, coming from Nigeria but living in Italy, came to our unit claiming to have persistent pain and combined median and ulnar palsy. Under suspicion of leprosy, skin and left sural nerve biopsy were performed. Skin tests were negative, but Schwann cells resulted as positive for acid-fast bacilli (AFB), leading to the diagnosis of Pure Neuritic Leprosy (PNL). The patient was given PB multidrug therapy and recovered from pain in two months...
2016: Case Reports in Neurological Medicine
Sanjay Khadgi, Babu Shrestha, Hamdy Ibrahim, Sunil Shrestha, Mohammed S ElSheemy, Ahmed M Al-Kandari
To evaluate safety and efficacy of minipercutaneous nephrolithotomy (Mini-PNL) in management of stones in different types of renal anomalies. Patients with stones ≥2 cm or SWL-resistant stones in anomalous-kidneys treated by Mini-PNL between March 2010 and September 2012 were included prospectively. Mini-PNL was done under regional anesthesia in prone position with fluoroscopic guidance through 18 Fr sheath using semirigid ureteroscope (8.5/11.5 Fr) and pneumatic lithotripter. All patients were followed-up for 2-3 years...
October 4, 2016: Urolithiasis
Hüseyin Buğra Karakaş, İzzet Çiçekbilek, Adem Tok, Tamer Alışkan, Bülent Akduman
OBJECTIVE: In this study we aimed to evaluate intraoperative and postoperative complications which developed according to pre-operative American Society of Anesthesiologists (ASA) risk criteria in patients who had undergone percutaneous nephrolithotomy (PNL). MATERIAL AND METHODS: Five hundred and sixty patients who had undergone PNL between 2002 and 2014 were included in the study. Patients operated on the ipsilateral kidney, those with solitary kidney or the cases who had previously undergone more than one access were excluded from this study...
September 2016: Turkish Journal of Urology
İbrahim Buldu, Abdulkadir Tepeler, Tuna Karatağ, Ramazan İnan, Abdullah Armağan, Okan İstanbulluoğlu
OBJECTIVE: We aimed to present the technique of combination of standard percutaneous nephrolithotomy (PNL) with microperc for achieving higher success rates without increasing complication rates in the management of complex renal calculi. MATERIAL AND METHODS: The patients who underwent microperc procedure as a complementary procedure to standard PNL for complex kidney stones in two reference hospitals between 2013 and 2015, were evaluated retrospectively. RESULTS: All patients underwent a total of two accesses one for standard PNL and one for microperc...
September 2016: Turkish Journal of Urology
A Miernik, S Hein, F Adams, J Halbritter, M Schoenthaler
Urological and surgical treatment of urinary stones are highly technological and technology-driven disciplines in present-day surgery. German medical engineering has always been recognized for its technical innovations in endoscopic surgery. Current and future trends are indicative of further miniaturization and automation of surgical instruments and assist systems to facilitate endourological procedures as well as improvements in the quality of results and ergonomics. These technologies include, e. g. 3D-tracking to facilitate access to the pelvicaliceal system for percutaneous nephrolithotomy (PNL) or robotic master-slave systems for endourology...
October 2016: Der Urologe. Ausg. A
Sun Woo Kim, Ji Hoon Ahn, Sang Un Yim, Yang Hyun Cho, Bo Sung Shin, Ho Seok Chung, Eu Chang Hwang, Ho Song Yu, Kyung Jin Oh, Sun-Ouck Kim, Seung Il Jung, Taek Won Kang, Dong Deuk Kwon, Kwangsung Park
PURPOSE: This study aimed to determine the predictors of ipsilateral hydronephrosis after ureteroscopic lithotripsy for ureteral calculi. MATERIALS AND METHODS: From January 2010 to December 2014, a total of 204 patients with ureteral calculi who underwent ureteroscopic lithotripsy were reviewed. Patients with lack of clinical data, presence of ureteral rupture, and who underwent simultaneous percutaneous nephrolithotomy (PNL) were excluded. Postoperative hydronephrosis was determined via computed tomographic scan or renal ultrasonography, at 6 months after ureteroscopic lithotripsy...
September 2016: Investigative and Clinical Urology
Emrah Yuruk, Gokhan Gureser, Murat Tuken, Kasim Ertas, Ege Can Serefoglu
INTRODUCTION: The aim of this study was to demonstrate the efficacy of Instadose™, a novel dosimeter designed for radiation workers to provide a measurement of the radiation dose at any time from any computer; to determine the amount of radiation exposure during percutaneous nephrolithotomy (PNL); and to evaluate the factors that affect the amount of radiation exposed. MATERIAL AND METHODS: Two experienced surgeons wore Instadose™ on the outer part of their lead aprons during the PNL procedures performed between December 2013 and July 2014...
2016: Central European Journal of Urology
Santosh Kumar, Shivanshu Singh, Prashant Singh, Shrawan Kumar Singh
INTRODUCTION: To compare the outcomes of tubeless day care PNL using hemostatic seal in the access tract versus standard PNL. MATERIAL AND METHODS: It was a prospective randomized controlled study. Cases were randomized to either the day care group with hemostatic seal (DCS) or the control group where patients were admitted and a nephrostomy tube was placed at the conclusion of surgery. RESULTS: A total of 180 cases were screened and out of these, 113 were included in the final analysis...
2016: Central European Journal of Urology
Guilherme Ribeiro Romualdo, Renata Leme Goto, Ana Angélica Henrique Fernandes, Bruno Cogliati, Luis Fernando Barbisan
Although there is a concomitance of zinc deficiency and high incidence/mortality for hepatocellular carcinoma in certain human populations, there are no experimental studies investigating the modifying effects of zinc on hepatocarcinogenesis. Thus, we evaluated whether dietary zinc deficiency or supplementation alter the development of hepatocellular preneoplastic lesions (PNL). Therefore, neonatal male Balb/C mice were submitted to a diethylnitrosamine/2-acetylaminefluorene-induced hepatocarcinogenesis model...
October 2016: Food and Chemical Toxicology
Fatih Akbulut, Onur Kucuktopcu, Emre Kandemir, Burak Ucpinar, Faruk Ozgor, Abdulmuttalip Simsek, Burak Arslan, Akif Erbin, Fatih Yanaral, Murat Binbay, Gokhan Gurbuz
PURPOSE: We aimed to evaluate the effect of obesity on surgical outcomes of mini percutaneous nephrolithotomy (Mini-PNL). METHODS: Hundred and eighty two Mini-PNL procedures were performed between May 2013 and January 2015 and their results were evaluated retrospectively. Patients were classified as non-obese (BMI, 18.5-30 kg/m(2)) and obese (≥30 kg/m(2)) groups. Obese and non-obese patients were compared according to pre-operative demographic values, intra-operative surgery techniques and post-operative results...
2016: SpringerPlus
Tsu-Ming Chien, Yen-Man Lu, Yii-Her Chou, Wen-Jeng Wu, Chun-Nung Huang
OBJECTIVE: To determine whether percutaneous nephrolithotomy or ureteroscopic lithotripsy lead to the development of hypertension, using the Taiwan National Health Insurance database. METHODS: Data were sourced from the Longitudinal Health Insurance Database (LHID2000) of Taiwan, Republic of China, compiled by the Taiwan National Health Insurance database from 1996 to 2010. Percutaneous nephrolithotomy and ureteroscopic lithotripsy were studied as a time-dependent covariate in a Cox proportional hazard model to estimate the hazard ratio for the effect of new onset hypertension...
August 2, 2016: Urology
Huseyin Celik, Ahmet Camtosun, Ramazan Altintas, Cemal Tasdemir
OBJECTIVE: Pediatric stone disease is a significant health issue which has increased in incidence because of lifestyle changes, dietary habits, and obesity. The incidence of urolithiasis among pediatric age groups varies according to region and is high in Turkey. The management of stone disease in children has improved dramatically over the last two decades. The high success rate of percutaneous nephrolithotomy (PNL) have led modern researchers to suggest that it be used as a first-line treatment of kidney stones greater than 2 cm in size...
July 17, 2016: Journal of Pediatric Urology
Ramazan Kocaaslan, Abdulkadir Tepeler, Ibrahim Buldu, Muhammed Tosun, Mehmet Mazhar Utangac, Tolga Karakan, Ekrem Ozyuvali, Namik Kemal Hatipoglu, Ali Unsal, Kemal Sarica
The objective of this study is to assess the utility of the Guy, S.T.O.N.E., and CROES nephrolithometry scoring systems (SS), and compare the capability of each system to predict percutaneous nephrolithotomy (PNL) outcome in patients with anatomical abnormalities. We retrospectively collected medical records of patients with anatomical abnormalities who underwent PNL for the treatment of renal calculi by experienced surgical teams in four referral centers. All of the patients were graded by a single observer from each department based on preoperative computed tomography images using each SS...
July 12, 2016: Urolithiasis
Saima Rehman, Haq Nawaz Bhatti, Muhammad Bilal, Muhammad Asgher
Cross-linked enzyme aggregates (CLEAs) are considered as an effective tool for the immobilization of enzyme. In this study, Pencillium notatum lipase (PNL) was immobilized as carrier free cross-linked enzyme aggregates using glutaraldehyde (GLA) and Ethylene glycol-bis [succinic acid N-hydroxysuccinimide] (EG-NHS) as cross-linking agents. The optimal conditions for the synthesis of an efficient lipase CLEAs such as precipitant type, the nature and amount of cross-linking reagent, and cross-linking time were optimized...
October 2016: International Journal of Biological Macromolecules
Ruth Pape, Kelly Maree Spuur, Geoffrey Currie, Lacey Greene
INTRODUCTION: The objective of the study was to document the distribution of mammographic parenchymal patterns (MPP) of Indigenous Australian women attending BreastScreen New South Wales (NSW) North Coast, to profile breast cancer risk as it relates to breast density and to explore the correlation between MPP, breast size as described by the posterior nipple line (PNL) and age. METHODS: Ethics was granted from CQUniversity Human Research Ethics Committee, NSW Population Health Services Research Ethics Committee and the Aboriginal Health and Medical Research Council Ethics Committee...
June 2016: Journal of Medical Radiation Sciences
Chi Xu, Lilian Zhang, Li Cheng, Reinhard Koch
In this paper we deal with the camera pose estimation problem from a set of 2D/3D line correspondences, which is also known as PnL (Perspective-n-Line) problem. We carry out our study by comparing PnL with the well-studied PnP (Perspective-n-Point) problem, and our contributions are threefold: (1) We provide a complete 3D configuration analysis for P3L, which includes the well-known P3P problem as well as several existing analyses as special cases. (2) By exploring the similarity between PnL and PnP, we propose a new subset-based PnL approach as well as a series of linear-formulation-based PnL approaches inspired by their PnP counterparts...
June 20, 2016: IEEE Transactions on Pattern Analysis and Machine Intelligence
Tufan Süelözgen, Cemal Selcuk Isoglu, Hakan Turk, Mehmet Yoldas, Mustafa Karabicak, Batuhan Ergani, Hayal Boyacioglu, Yusuf Ozlem Ilbey, Ferruh Zorlu
INTRODUCTION: This study aimed to evaluate whether one-shot dilatation technique is as safe in patients with a history of open-stone surgery as it is in patients without previous open-stone surgery. METHODS: Between January 2007 and February 2015, 82 patients who underwent percutaneous nephrolithotomy (PNL) surgery with one-shot dilation technique who previously had open-stone surgery were retrospectively reviewed and evaluated (Group 1). Another 82 patients were selected randomly among patients who had PNL with one-shot dilation technique, but with no history of open renal surgery (Group 2)...
March 2016: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
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