keyword
MENU ▼
Read by QxMD icon Read
search

Msn2

keyword
https://www.readbyqxmd.com/read/28924051/the-stress-regulatory-transcription-factors-msn2-and-msn4-regulate-fatty-acid-oxidation-in-budding-yeast
#1
Praveen Kumar Rajvanshi, Madhuri Arya, Ram Rajasekharan
The transcription factors multicopy suppressor of SNF1 mutation (Msn) Msn2 and Msn4 bind the stress-response element in gene promoters in the yeast Saccharomyces cerevisiae. However, the roles of Msn2/4 in primary metabolic pathways such as fatty acid β-oxidation are unclear. Here, in silico analysis revealed that the promoters of most genes involved in the biogenesis, function, and regulation of the peroxisome contain Msn2/4 binding sites. We also found that transcript levels of MSN2/MSN4 are increased in glucose-depletion conditions and that during growth in nonpreferred carbon sources, Msn2 is constantly localized to the nucleus in wildtype cells...
September 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28760824/the-glucose-sensor-snf1-and-the-transcription-factors-msn2-and-msn4-regulate-transcription-of-the-vacuolar-iron-importer-gene-ccc1-and-iron-resistance-in-yeast
#2
Liangtao Li, Jerry Kaplan, Diane M Ward
The budding yeast Saccharomyces cerevisiae stores iron in the vacuole, which is a major resistance mechanism against iron toxicity. One key protein involved in vacuolar iron storage is the iron importer Ccc1, which facilitates iron entry into the vacuole. Transcription of the CCC1 gene is largely regulated by the binding of iron-sulfur clusters to the activator domain of the transcriptional activator Yap5. Additional evidence, however, suggests that Yap5-independent transcriptional activation of CCC1 also contributes to iron resistance...
September 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28745839/transcription-factor-agseb1-affects-development-osmotic-stress-response-and-secondary-metabolism-in-marine-derived-aspergillus-glaucus
#3
Yuan Wu, Yanna Ren, Xiangshan Zhou, Menghao Cai, Yuanxing Zhang
Fungi possess sophisticated regulatory systems to respond to a vast array of environmental signals. Among these responsive networks, some genes play critical roles in the regulation of various cellular processes. Here, we identified a putative transcriptional factor Agseb1 in Aspergillus glaucus, a marine-derived filamentous fungus. Agseb1 encodes a protein with two C2 H2 zinc fingers at the C-terminus, similar to the placement of these motifs in msn2/4 of Saccharomyces cerevisia, where they are positioned to allow binding to the CCCCT-box of stress-specific genes...
July 26, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28720901/a-novel-connection-between-the-cell-wall-integrity-and-the-pka-pathways-regulates-cell-wall-stress-response-in-yeast
#4
Raúl García, Enrique Bravo, Sonia Diez-Muñiz, Cesar Nombela, Jose M Rodríguez-Peña, Javier Arroyo
Fungal cells trigger adaptive mechanisms to survive in situations that compromise cell wall integrity. We show here that the global transcriptional response elicited by inhibition of the synthesis of β-1,3-glucan by caspofungin, encompasses a set of genes that are dependent on Slt2, the MAPK of the Cell Wall Integrity (CWI) pathway, and a broad group of genes regulated independently of Slt2. Genes negatively regulated by the cyclic AMP/Protein Kinase A (PKA) signaling pathway were overrepresented in the latter group...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28676626/yeast-cip1-is-activated-by-environmental-stress-to-inhibit-cdk1-g1-cyclins-via-mcm1-and-msn2-4
#5
Ya-Lan Chang, Shun-Fu Tseng, Yu-Ching Huang, Zih-Jie Shen, Pang-Hung Hsu, Meng-Hsun Hsieh, Chia-Wei Yang, Silvia Tognetti, Berta Canal, Laia Subirana, Chien-Wei Wang, Hsiao-Tan Chen, Chi-Ying Lin, Francesc Posas, Shu-Chun Teng
Upon environmental changes, proliferating cells delay cell cycle to prevent further damage accumulation. Yeast Cip1 is a Cdk1 and Cln2-associated protein. However, the function and regulation of Cip1 are still poorly understood. Here we report that Cip1 expression is co-regulated by the cell-cycle-mediated factor Mcm1 and the stress-mediated factors Msn2/4. Overexpression of Cip1 arrests cell cycle through inhibition of Cdk1-G1 cyclin complexes at G1 stage and the stress-activated protein kinase-dependent Cip1 T65, T69, and T73 phosphorylation may strengthen the Cip1and Cdk1-G1 cyclin interaction...
July 4, 2017: Nature Communications
https://www.readbyqxmd.com/read/28646136/recurrent-background-mutations-in-whi2-impair-proteostasis-and-degradation-of-misfolded-cytosolic-proteins-in-saccharomyces-cerevisiae
#6
Sophie A Comyn, Stéphane Flibotte, Thibault Mayor
Proteostasis promotes viability at both the cellular and organism levels by maintaining a functional proteome. This requires an intricate protein quality control (PQC) network that mediates protein folding by molecular chaperones and removes terminally misfolded proteins via the ubiquitin proteasome system and autophagy. How changes within the PQC network can perturb proteostasis and shift the balance between protein folding and proteolysis remain poorly understood. However, given that proteostasis is altered in a number of conditions such as cancer and ageing, it is critical that we identify the factors that mediate PQC and understand the interplay between members of the proteostatic network...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28637875/coupled-feedback-loops-control-the-stimulus-dependent-dynamics-of-the-yeast-transcription-factor-msn2
#7
Yanfei Jiang, Zohreh AkhavanAghdam, Lev S Tsimring, Nan Hao
Information about environmental stimuli often can be encoded by the dynamics of signaling molecules or transcription factors. In the yeast Saccharomyces cerevisiae, different types of stresses induce distinct nuclear translocation dynamics of the general stress-responsive transcription factor Msn2, but the underlying mechanisms remain unclear. Using deterministic and stochastic modeling, we reproduced in silico the different dynamic responses of Msn2 to glucose limitation and osmotic stress observed in vivo and found that a positive feedback loop on protein kinase A mediated by the AMP-activated protein kinase Snf1 is coupled with a negative feedback loop to generate the characteristic pulsatile dynamics of Msn2...
July 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28457997/absence-of-gim-proteins-but-not-gimc-complex-alters-stress-induced-transcription
#8
Ana Fátima Amorim, Dora Pinto, Laurent Kuras, Lisete Fernandes
Saccharomyces cerevisiae GimC (mammalian Prefoldin) is a hexameric (Gim1-6) cytoplasmic complex involved in the folding pathway of actin/tubulin. In contrast to a shared role in GimC complex, we show that absence of individual Gim proteins results in distinct stress responses. No concomitant alteration in F-actin integrity was observed. Transcription of stress responsive genes is altered in gim2Δ, gim3Δ and gim6Δ mutants: TRX2 gene is induced in these mutants but with a profile diverging from type cells, whereas CTT1 and HSP26 fail to be induced...
April 28, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28358805/fasting-regulates-egr1-and-protects-from-glucose-and-dexamethasone-dependent-sensitization-to-chemotherapy
#9
Stefano Di Biase, Hong Seok Shim, Kyung Hwa Kim, Manlio Vinciguerra, Francesca Rappa, Min Wei, Sebastian Brandhorst, Francesco Cappello, Hamed Mirzaei, Changhan Lee, Valter D Longo
Fasting reduces glucose levels and protects mice against chemotoxicity, yet drugs that promote hyperglycemia are widely used in cancer treatment. Here, we show that dexamethasone (Dexa) and rapamycin (Rapa), commonly administered to cancer patients, elevate glucose and sensitize cardiomyocytes and mice to the cancer drug doxorubicin (DXR). Such toxicity can be reversed by reducing circulating glucose levels by fasting or insulin. Furthermore, glucose injections alone reversed the fasting-dependent protection against DXR in mice, indicating that elevated glucose mediates, at least in part, the sensitizing effects of rapamycin and dexamethasone...
March 2017: PLoS Biology
https://www.readbyqxmd.com/read/28337980/light-sensing-via-hydrogen-peroxide-and-a-peroxiredoxin
#10
Kristofer Bodvard, Ken Peeters, Friederike Roger, Natalie Romanov, Aeid Igbaria, Niek Welkenhuysen, Gaël Palais, Wolfgang Reiter, Michel B Toledano, Mikael Käll, Mikael Molin
Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner...
March 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28330760/ck2-dependent-phosphorylation-positively-regulates-stress-induced-activation-of-msn2-in-saccharomyces-cerevisiae
#11
Bo-Ram Cho, Ji-Sook Hahn
CK2 is a highly conserved Ser/Thr protein kinase involved in a large number of cellular processes. Here, we demonstrate that CK2-dependent phosphorylation positively regulates Msn2/4, the general stress response transcriptional activators in Saccharomyces cerevisiae, in response to various types of environmental stress conditions. CK2 overexpression elicits hyperactivation of Msn2/4, whereas deletion of one of the CK2 catalytic subunits, especially CKA2, leads to reduced transcriptional activity of Msn2/4 in response to glucose starvation, H2O2, and lactic acid...
June 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28209126/a-meta-analysis-reveals-complex-regulatory-properties-at-taf14-repressed-genes
#12
Josipa Nemet, Nikolina Vidan, Mary Sopta
BACKGROUND: Regulation of gene transcription in response to stress is central to a cell's ability to cope with environmental challenges. Taf14 is a YEATS domain protein in S.cerevisiae that physically associates with several transcriptionally relevant multisubunit complexes including the general transcription factors TFIID and TFIIF and the chromatin-modifying complexes SWI/SNF, INO80, RSC and NuA3. TAF14 deletion strains are sensitive to a variety of stresses suggesting that it plays a role in the transcriptional stress response...
February 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/27690227/dynamic-control-of-gene-regulatory-logic-by-seemingly-redundant-transcription-factors
#13
Zohreh AkhavanAghdam, Joydeb Sinha, Omar P Tabbaa, Nan Hao
Many transcription factors co-express with their homologs to regulate identical target genes, however the advantages of such redundancies remain elusive. Using single-cell imaging and microfluidics, we study the yeast general stress response transcription factor Msn2 and its seemingly redundant homolog Msn4. We find that gene regulation by these two factors is analogous to logic gate systems. Target genes with fast activation kinetics can be fully induced by either factor, behaving as an 'OR' gate. In contrast, target genes with slow activation kinetics behave as an 'AND' gate, requiring distinct contributions from both factors, upon transient stimulation...
September 30, 2016: ELife
https://www.readbyqxmd.com/read/27628486/costs-benefits-and-redundant-mechanisms-of-adaption-to-chronic-low-dose-stress-in-yeast
#14
Marta Markiewicz-Potoczny, David Lydall
All organisms live in changeable, stressful environments. It has been reported that exposure to low-dose stresses or poisons can improve fitness. However, examining the effects of chronic low-dose chemical exposure is challenging. To address this issue we used temperature sensitive mutations affecting the yeast cell division cycle to induce low-dose stress for 40 generation times, or more. We examined cdc13-1 mutants, defective in telomere function, and cdc15-2 mutants, defective in mitotic kinase activity...
October 17, 2016: Cell Cycle
https://www.readbyqxmd.com/read/27618436/the-9aatad-transactivation-domains-from-gal4-to-p53
#15
Martin Piskacek, Marek Havelka, Martina Rezacova, Andrea Knight
The family of the Nine amino acid Transactivation Domain, 9aaTAD family, comprises currently over 40 members. The 9aaTAD domains are universally recognized by the transcriptional machinery from yeast to man. We had identified the 9aaTAD domains in the p53, Msn2, Pdr1 and B42 activators by our prediction algorithm. In this study, their competence to activate transcription as small peptides was proven. Not surprisingly, we elicited immense 9aaTAD divergence in hundreds of identified orthologs and numerous examples of the 9aaTAD species' convergence...
2016: PloS One
https://www.readbyqxmd.com/read/27478089/attenuation-of-transcriptional-and-signaling-responses-limits-viability-of-%C3%AF-0-saccharomyces-cerevisiae-during-periods-of-glucose-deprivation
#16
R Magnus N Friis, Michael C Schultz
BACKGROUND: The maintenance of viability during periods when a glycolytic carbon source is limited (or absent) is a major obstacle for cells whose mitochondrial DNA (mtDNA) has been damaged or lost. METHODS: We utilized genome wide transcriptional profiling and in gel mobility analyses to examine the transcriptional response and characterize defects in the phosphorylation dependent signaling events that occur during acute glucose starvation in ρ(0) cells that lack mtDNA...
November 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27325672/oma1-links-mitochondrial-protein-quality-control-and-tor-signaling-to-modulate-physiological-plasticity-and-cellular-stress-responses
#17
Iryna Bohovych, Stavroula Kastora, Sara Christianson, Danelle Topil, Heejeong Kim, Teresa Fangman, You J Zhou, Antoni Barrientos, Jaekwon Lee, Alistair J P Brown, Oleh Khalimonchuk
A network of conserved proteases known as the intramitochondrial quality control (IMQC) system is central to mitochondrial protein homeostasis and cellular health. IMQC proteases also appear to participate in establishment of signaling cues for mitochondrion-to-nucleus communication. However, little is known about this process. Here, we show that in Saccharomyces cerevisiae, inactivation of the membrane-bound IMQC protease Oma1 interferes with oxidative-stress responses through enhanced production of reactive oxygen species (ROS) during logarithmic growth and reduced stress signaling via the TORC1-Rim15-Msn2/Msn4 axis...
September 1, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27235439/evidence-for-a-role-for-the-plasma-membrane-in-the-nanomechanical-properties-of-the-cell-wall-as-revealed-by-an-atomic-force-microscopy-study-of-the-response-of-saccharomyces-cerevisiae-to-ethanol-stress
#18
Marion Schiavone, Cécile Formosa-Dague, Carolina Elsztein, Marie-Ange Teste, Helene Martin-Yken, Marcos A De Morais, Etienne Dague, Jean M François
UNLABELLED: A wealth of biochemical and molecular data have been reported regarding ethanol toxicity in the yeast Saccharomyces cerevisiae However, direct physical data on the effects of ethanol stress on yeast cells are almost nonexistent. This lack of information can now be addressed by using atomic force microscopy (AFM) technology. In this report, we show that the stiffness of glucose-grown yeast cells challenged with 9% (vol/vol) ethanol for 5 h was dramatically reduced, as shown by a 5-fold drop of Young's modulus...
August 1, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27092438/ionic-and-thermo-switchable-polymer-masked-mesoporous-silica-drug-nanocarrier-high-drug-loading-capacity-at-10%C3%A2-c-and-fast-drug-release-completion-at-40%C3%A2-c
#19
Mohamed Eltohamy, Jae-Won Seo, Ji-Young Hwang, Won-Cheoul Jang, Hae-Won Kim, Ueon Sang Shin
The preparation of the ideal smart drug-delivery systems were successfully achieved by the in situ co-polymerization of a vinyl group-functionalized mesoporous silica nanoparticle (f-MSN) with 1-butyl-3-vinyl imidazolium bromide (BVIm) and N-isopropylacrylamide (NIPAAm) monomers. The thickness of the capping copolymer layer, poly(NIPAAm-co-BVIm) (p-NIBIm), was controlled at between 2.5nm and 5nm, depending on the monomers/f-MSN ratio in the reaction solution. The finally obtained smart drug-delivery systems are named as p-MSN2...
August 1, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/26947009/the-signaling-pathways-underlying-starvation-induced-upregulation-of-%C3%AE-mannosidase-ams1-in-saccharomyces-cerevisiae
#20
Midori Umekawa, Masato Ujihara, Kazuki Makishima, Shohei Yamamoto, Hiromu Takematsu, Mamoru Wakayama
BACKGROUND: Cells have evolved the mechanisms to survive nutritional shortages in the environment. In Saccharomyces cerevisiae, α-mannosidase Ams1 is known to play a role in catabolism of N-linked free oligosaccharides in the cytosol. Although, this enzyme is also known to be transported selectively from the cytosol to the vacuoles by autophagy, the physiological significance of this transport has not been clarified. METHODS: To elucidate the regulatory mechanism of the activity of Ams1, we assessed the enzymatic activity of the cell free extract of the wild-type and various gene disruptants under different nutritional conditions...
June 2016: Biochimica et Biophysica Acta
keyword
keyword
22627
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"