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https://www.readbyqxmd.com/read/27827352/crystal-structures-of-a-yeast-14-3-3-protein-from-lachancea-thermotolerans-in-the-unliganded-form-and-bound-to-a-human-lipid-kinase-pi4kb-derived-peptide-reveal-high-evolutionary-conservation
#1
Andrea Eisenreichova, Martin Klima, Evzen Boura
14-3-3 proteins bind phosphorylated binding partners to regulate several of their properties, including enzymatic activity, stability and subcellular localization. Here, two crystal structures are presented: the crystal structures of the 14-3-3 protein (also known as Bmh1) from the yeast Lachancea thermotolerans in the unliganded form and bound to a phosphopeptide derived from human PI4KB (phosphatidylinositol 4-kinase B). The structures demonstrate the high evolutionary conservation of ligand recognition by 14-3-3 proteins...
November 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27159239/a-fret-based-study-reveals-site-specific-regulation-of-spindle-position-checkpoint-proteins-at-yeast-centrosomes
#2
Yuliya Gryaznova, Ayse Koca Caydasi, Gabriele Malengo, Victor Sourjik, Gislene Pereira
The spindle position checkpoint (SPOC) is a spindle pole body (SPB, equivalent of mammalian centrosome) associated surveillance mechanism that halts mitotic exit upon spindle mis-orientation. Here, we monitored the interaction between SPB proteins and the SPOC component Bfa1 by FRET microscopy. We show that Bfa1 binds to the scaffold-protein Nud1 and the γ-tubulin receptor Spc72. Spindle misalignment specifically disrupts Bfa1-Spc72 interaction by a mechanism that requires the 14-3-3-family protein Bmh1 and the MARK/PAR-kinase Kin4...
2016: ELife
https://www.readbyqxmd.com/read/27096577/kin2-the-budding-yeast-ortholog-of-animal-mark-par-1-kinases-localizes-to-the-sites-of-polarized-growth-and-may-regulate-septin-organization-and-the-cell-wall
#3
Si-Min Yuan, Wen-Chao Nie, Fei He, Zhi-Wen Jia, Xiang-Dong Gao
MARK/PAR-1 protein kinases play important roles in cell polarization in animals. Kin1 and Kin2 are a pair of MARK/PAR-1 orthologs in the budding yeast Saccharomyces cerevisiae. They participate in the regulation of secretion and ER stress response. However, neither the subcellular localization of these two kinases nor whether they may have other cellular functions is clear. Here, we show that Kin2 localizes to the sites of polarized growth in addition to localization on the plasma membrane. The localization to polarity sites is mediated by two targeting domains-TD1 and TD2...
2016: PloS One
https://www.readbyqxmd.com/read/27001512/the-hsp70-homolog-ssb-and-the-14-3-3-protein-bmh1-jointly-regulate-transcription-of-glucose-repressed-genes-in-saccharomyces-cerevisiae
#4
Volker Hübscher, Kaivalya Mudholkar, Marco Chiabudini, Edith Fitzke, Tina Wölfle, Dietmar Pfeifer, Friedel Drepper, Bettina Warscheid, Sabine Rospert
Chaperones of the Hsp70 family interact with a multitude of newly synthesized polypeptides and prevent their aggregation. Saccharomyces cerevisiae cells lacking the Hsp70 homolog Ssb suffer from pleiotropic defects, among others a defect in glucose-repression. The highly conserved heterotrimeric kinase SNF1/AMPK (AMP-activated protein kinase) is required for the release from glucose-repression in yeast and is a key regulator of energy balance also in mammalian cells. When glucose is available the phosphatase Glc7 keeps SNF1 in its inactive, dephosphorylated state...
July 8, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/26832405/fin1-pp1-helps-clear-spindle-assembly-checkpoint-protein-bub1-from-kinetochores-in-anaphase
#5
Michael Bokros, Curtis Gravenmier, Fengzhi Jin, Daniel Richmond, Yanchang Wang
The spindle assembly checkpoint (SAC) monitors chromosome attachment defects, and the assembly of SAC proteins at kinetochores is essential for its activation, but the SAC disassembly process remains unknown. We found that deletion of a 14-3-3 protein, Bmh1, or hyperactivation of Cdc14 early anaphase release (FEAR) allows premature SAC silencing in budding yeast, which depends on a kinetochore protein Fin1 that forms a complex with protein phosphatase PP1. Previous works suggest that FEAR-dependent Fin1 dephosphorylation promotes Bmh1-Fin1 dissociation, which enables kinetochore recruitment of Fin1-PP1...
February 9, 2016: Cell Reports
https://www.readbyqxmd.com/read/25355315/yeast-14-3-3-protein-functions-as-a-comodulator-of-transcription-by-inhibiting-coactivator-functions
#6
Pabitra K Parua, Kenneth M Dombek, Elton T Young
In eukaryotes combinatorial activation of transcription is an important component of gene regulation. In the budding yeast Saccharomyces cerevisiae, Adr1-Cat8 and Adr1-Oaf1/Pip2 are pairs of activators that act together to regulate two diverse sets of genes. Transcription activation of both sets is regulated positively by the yeast AMP-activated protein kinase homolog, Snf1, in response to low glucose or the presence of a non-fermentable carbon source and negatively by two redundant 14-3-3 isoforms, Bmh1 and Bmh2...
December 19, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25315811/a-comprehensive-proteomic-and-phosphoproteomic-analysis-of-yeast-deletion-mutants-of-14-3-3-orthologs-and-associated-effects-of-rapamycin
#7
Joao A Paulo, Steven P Gygi
We applied a multiplexed, MS-based strategy to interrogate the proteome and phosphoproteome of three yeast strains under two growth conditions in triplicate. The yeast proteins brain modulosignalin homologue (Bmh)1 and Bmh2, analogs to the 14-3-3 protein family, have a wide array of cellular functions including the regulation of phosphorylation events. Moreover, rapamycin is a drug that can regulate phosphorylation events. By performing a series of tandem mass tag 10-plex experiments, we investigated the alterations in the proteome and phosphoproteome of wildtype and two deletion strains (bmh1Δ and bmh2Δ) of Saccharomyces cerevisiae treated with rapamycin and DMSO as a control...
January 2015: Proteomics
https://www.readbyqxmd.com/read/25315061/unveiling-equal-importance-of-two-14-3-3-proteins-for-morphogenesis-conidiation-stress-tolerance-and-virulence-of-an-insect-pathogen
#8
Qian Liu, Jin-Gen Li, Sheng-Hua Ying, Juan-Juan Wang, Wen-Liang Sun, Chao-Guang Tian, Ming-Guang Feng
Two conserved 14-3-3 proteins orthologous to Saccharomyces cerevisiae Bmh1/2 are poorly understood in filamentous fungi. Here we show that Bmh1 and Bmh2 contribute equally to the fundamental biology and physiology of Beauveria bassiana by targeting many sets of proteins/enzymes. Single Bmh deletion caused similar upregulation of another. Excellent knockdown (∼91%) expressions of Bmh1 in ΔBmh2 and Bmh2 in ΔBmh1 resulted in equally more severe multiphenotypic defects than the single deletions, including G2 /M transition, blastospore size, carbon/nitrogen utilization, conidiation, germination and conidial tolerances to high osmolarity, oxidation, cell wall stress, high temperature and UV-B irradiation...
April 2015: Environmental Microbiology
https://www.readbyqxmd.com/read/24850890/the-14-3-3-protein-bmh1-functions-in-the-spindle-position-checkpoint-by-breaking-bfa1-asymmetry-at-yeast-centrosomes
#9
Ayse Koca Caydasi, Yagmur Micoogullari, Bahtiyar Kurtulmus, Saravanan Palani, Gislene Pereira
In addition to their well-known role in microtubule organization, centrosomes function as signaling platforms and regulate cell cycle events. An important example of such a function is the spindle position checkpoint (SPOC) of budding yeast. SPOC is a surveillance mechanism that ensures alignment of the mitotic spindle along the cell polarity axis. Upon spindle misalignment, phosphorylation of the SPOC component Bfa1 by Kin4 kinase engages the SPOC by changing the centrosome localization of Bfa1 from asymmetric (one centrosome) to symmetric (both centrosomes)...
July 15, 2014: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/24605246/adenosine-triphosphate-atp-is-a-candidate-signaling-molecule-in-the-mitochondria-to-nucleus-retrograde-response-pathway
#10
Feng Zhang, Tammy Pracheil, Janet Thornton, Zhengchang Liu
Intracellular communication from the mitochondria to the nucleus is achieved via the retrograde response. In budding yeast, the retrograde response, also known as the RTG pathway, is regulated positively by Rtg1, Rtg2, Rtg3 and Grr1 and negatively by Mks1, Lst8 and two 14-3-3 proteins, Bmh1/2. Activation of retrograde signaling leads to activation of Rtg1/3, two basic helix-loop-helix leucine zipper transcription factors. Rtg1/3 activation requires Rtg2, a cytoplasmic protein with an N-terminal adenosine triphosphate (ATP) binding domain belonging to the actin/Hsp70/sugar kinase superfamily...
March 2013: Genes
https://www.readbyqxmd.com/read/24438204/the-yeast-14-3-3-proteins-bmh1-and-bmh2-differentially-regulate-rapamycin-mediated-transcription
#11
Michael A Trembley, Hunter L Berrus, Jonathan R Whicher, Emily L Humphrey-Dixon
14-3-3 proteins are highly conserved and have been found in all eukaryotic organisms investigated. They are involved in many varied cellular processes, and interact with hundreds of other proteins. Among many other roles in cells, yeast 14-3-3 proteins have been implicated in rapamycin-mediated cell signaling. We determined the transcription profiles of bmh1 and bmh2 yeast after treatment with rapamycin. We found that, under these conditions, BMH1 and BMH2 are required for rapamycin-induced regulation of distinct, but overlapping sets of genes...
January 17, 2014: Bioscience Reports
https://www.readbyqxmd.com/read/24164389/phenotypic-and-genotypic-convergences-are-influenced-by-historical-contingency-and-environment-in-yeast
#12
Aymé Spor, Daniel J Kvitek, Thibault Nidelet, Juliette Martin, Judith Legrand, Christine Dillmann, Aurélie Bourgais, Dominique de Vienne, Gavin Sherlock, Delphine Sicard
Different organisms have independently and recurrently evolved similar phenotypic traits at different points throughout history. This phenotypic convergence may be caused by genotypic convergence and in addition, constrained by historical contingency. To investigate how convergence may be driven by selection in a particular environment and constrained by history, we analyzed nine life-history traits and four metabolic traits during an experimental evolution of six yeast strains in four different environments...
March 2014: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/23775924/multi-center-randomized-controlled-trial-of-cognitive-treatment-placebo-oxybutynin-bladder-training-and-pelvic-floor-training-in-children-with-functional-urinary-incontinence
#13
RANDOMIZED CONTROLLED TRIAL
Jan D van Gool, Tom P V M de Jong, Pauline Winkler-Seinstra, Tytti Tamminen-Möbius, Hildegard Lax, Herbert Hirche, Rien J M Nijman, Kelm Hjälmås, Ulf Jodal, Hannsjörg Bachmann, Piet Hoebeke, Johan Vande Walle, Joachim Misselwitz, Ulrike John, An Bael
OBJECTIVE: Functional urinary incontinence causes considerable morbidity in 8.4% of school-age children, mainly girls. To compare oxybutynin, placebo, and bladder training in overactive bladder (OAB), and cognitive treatment and pelvic floor training in dysfunctional voiding (DV), a multi-center controlled trial was designed, the European Bladder Dysfunction Study. METHODS: Seventy girls and 27 boys with clinically diagnosed OAB and urge incontinence were randomly allocated to placebo, oxybutynin, or bladder training (branch I), and 89 girls and 16 boys with clinically diagnosed DV to either cognitive treatment or pelvic floor training (branch II)...
June 2014: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/23726992/structural-basis-of-the-14-3-3-protein-dependent-activation-of-yeast-neutral-trehalase-nth1
#14
Eva Macakova, Miroslava Kopecka, Zdenek Kukacka, Dana Veisova, Petr Novak, Petr Man, Tomas Obsil, Veronika Obsilova
BACKGROUND: Trehalases are highly conserved enzymes catalyzing the hydrolysis of trehalose in a wide range of organisms. The activity of yeast neutral trehalase Nth1 is regulated in a 14-3-3- and a calcium-dependent manner. The Bmh proteins (the yeast 14-3-3 isoforms) recognize phosphorylated Nth1 and enhance its enzymatic activity through an unknown mechanism. METHODS: To investigate the structural basis of interaction between Nth1 and Bmh1, we used hydrogen/deuterium exchange coupled to mass spectrometry, circular dichroism spectroscopy and homology modeling to identify structural changes occurring upon the complex formation...
October 2013: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/23155055/in-vivo-phosphorylation-of-ser21-and-ser83-during-nutrient-induced-activation-of-the-yeast-protein-kinase-a-pka-target-trehalase
#15
Wim Schepers, Griet Van Zeebroeck, Martijn Pinkse, Peter Verhaert, Johan M Thevelein
The readdition of an essential nutrient to starved, fermenting cells of the yeast Saccharomyces cerevisiae triggers rapid activation of the protein kinase A (PKA) pathway. Trehalase is activated 5-10-fold within minutes and has been used as a convenient reporter for rapid activation of PKA in vivo. Although trehalase can be phosphorylated and activated by PKA in vitro, demonstration of phosphorylation during nutrient activation in vivo has been lacking. We now show, using phosphospecific antibodies, that glucose and nitrogen activation of trehalase in vivo is associated with phosphorylation of Ser(21) and Ser(83)...
December 28, 2012: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/22785534/14-3-3-protects-against-stress-induced-apoptosis
#16
C Clapp, L Portt, C Khoury, S Sheibani, G Norman, P Ebner, R Eid, H Vali, C A Mandato, F Madeo, M T Greenwood
Expression of human Bax, a cardinal regulator of mitochondrial membrane permeabilization, causes death in yeast. We screened a human cDNA library for suppressors of Bax-mediated yeast death and identified human 14-3-3β/α, a protein whose paralogs have numerous chaperone-like functions. Here, we show that, yeast cells expressing human 14-3-3β/α are able to complement deletion of the endogenous yeast 14-3-3 and confer resistance to a variety of different stresses including cadmium and cycloheximide. The expression of 14-3-3β/α also conferred resistance to death induced by the target of rapamycin inhibitor rapamycin and by starvation for the amino acid leucine, conditions that induce autophagy...
July 12, 2012: Cell Death & Disease
https://www.readbyqxmd.com/read/22484491/yeast-14-3-3-proteins-participate-in-the-regulation-of-cell-cation-homeostasis-via-interaction-with-nha1-alkali-metal-cation-proton-antiporter
#17
Jaromír Zahrádka, G Paul H van Heusden, Hana Sychrová
BACKGROUND: In yeast, 14-3-3 proteins bind to hundreds of phosphorylated proteins and play a role in the regulation of many processes including tolerance to NaCl. However, the mechanism of 14-3-3 involvement in the cell answer to salt or osmotic stresses is weakly understood. METHODS: We studied the role of the Saccharomyces cerevisiae 14-3-3 homologs Bmh1 and Bmh2 in the regulation of alkali-metal-cation homeostasis using the genetic-interaction approach. Obtained results were confirmed with the Bimolecular-Fluorescence-Complementation method...
July 2012: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/22320399/role-of-individual-phosphorylation-sites-for-the-14-3-3-protein-dependent-activation-of-yeast-neutral-trehalase-nth1
#18
Dana Veisova, Eva Macakova, Lenka Rezabkova, Miroslav Sulc, Petr Vacha, Hana Sychrova, Tomas Obsil, Veronika Obsilova
Trehalases are important highly conserved enzymes found in a wide variety of organisms and are responsible for the hydrolysis of trehalose that serves as a carbon and energy source as well as a universal stress protectant. Emerging evidence indicates that the enzymatic activity of the neutral trehalase Nth1 in yeast is enhanced by 14-3-3 protein binding in a phosphorylation-dependent manner through an unknown mechanism. In the present study, we investigated in detail the interaction between Saccharomyces cerevisiae Nth1 and 14-3-3 protein isoforms Bmh1 and Bmh2...
May 1, 2012: Biochemical Journal
https://www.readbyqxmd.com/read/21967861/differential-chromatin-proteomics-of-the-mms-induced-dna-damage-response-in-yeast
#19
Dong Ryoung Kim, Rohan D Gidvani, Brian P Ingalls, Bernard P Duncker, Brendan J McConkey
BACKGROUND: Protein enrichment by sub-cellular fractionation was combined with differential-in-gel-electrophoresis (DIGE) to address the detection of the low abundance chromatin proteins in the budding yeast proteome. Comparisons of whole-cell extracts and chromatin fractions were used to provide a measure of the degree of chromatin association for individual proteins, which could be compared across sample treatments. The method was applied to analyze the effect of the DNA damaging agent methyl methanesulfonate (MMS) on levels of chromatin-associated proteins...
2011: Proteome Science
https://www.readbyqxmd.com/read/21909961/induction-of-expression-of-a-14-3-3-gene-in-response-to-copper-exposure-in-the-marine-alga-fucus-vesiculosus
#20
Jennifer R Owen, Ceri A Morris, Beate Nicolaus, John L Harwood, Peter Kille
The macro-alga Fucus vesiculosus has a broad global and estuarine distribution and exhibits exceptional resistance to toxic metals, the molecular basis of which is poorly understood. To address this issue a cDNA library was constructed from an environmental isolate of F. vesiculosus growing in an area with chronic copper pollution. Characterisation of this library led to the identification of a cDNA encoding a protein known to be synthesised in response to toxicity, a full length 14-3-3 exhibiting a 71% identity to human/mouse epsilon isoform, 70-71% identity to yeast BMH1/2 and 95 and 71% identity to the Ectocarpus siliculosus 14-3-3 isoforms 1 and 2 respectively...
January 2012: Ecotoxicology
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