keyword
MENU ▼
Read by QxMD icon Read
search

H30-rx

keyword
https://www.readbyqxmd.com/read/29039274/different-escherichia-coli-b2-st131-clades-b-and-c-producing-extended-spectrum-%C3%AE-lactamases-esbl-colonizing-residents-of-portuguese-nursing-homes
#1
C Rodrigues, E Machado, S Fernandes, L Peixe, Â Novais
ESBL-producing Enterobacteriaceae and particularly Escherichia coli ST131 isolates producing CTX-M enzymes are commonly found colonizing the intestine of nursing home (NH) residents, but ST131 subclonal structure has been scarcely explored in this vulnerable population. Our goal was to perform a pilot study to assess the faecal carriage rate and epidemiological features of ESBL- and/or carbapenemase-producing Enterobacteriaceae (ESBL-E and CPE, respectively) among NH residents. For this purpose, faecal samples from residents at 4 different NHs in the North of Portugal (representing 9·5% of the residents' population, July 2014) were screened for ESBL-E and/or CPE by phenotypic and genotypic methods...
October 17, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28935873/whole-genome-sequencing-molecular-typing-and-in-vivo-virulence-of-oxa-48-producing-escherichia-coli-isolates-including-st131-h30-rx-h22-and-h41-subclones
#2
María de Toro, Javier Fernández, Vanesa García, Azucena Mora, Jorge Blanco, Fernando de la Cruz, M Rosario Rodicio
Carbapenem-resistant Enterobacteriaceae, including the increasingly reported OXA-48 Escherichia coli producers, are an emerging public health threat worldwide. Due to their alarming detection in our healthcare setting and their possible presence in the community, seven OXA-48-producing, extraintestinal pathogenic E. coli were analysed by whole genome sequencing as well as conventional tools, and tested for in vivo virulence. As a result, five E. coli OXA-48-producing subclones were detected (O25:H4-ST131/PST43-fimH30-virotype E; O25:H4-ST131/PST9-fimH22-virotype D5, O16:H5-ST131/PST506-fimH41; O25:H5-ST83/PST207 and O9:H25-ST58/PST24)...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28825894/adhesion-and-invasion-to-epithelial-cells-and-motility-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-reveal-st131-superiority-a-comparative-in-vitro-study-of-extraintestinal-pathogenic-e-coli-lineages
#3
Kira Kondratyeva, Ayala Wollman, Gabi Gerlitz, Shiri Navon-Venezia
PURPOSE: Extended-spectrum β-lactamase (ESBL)-producing extraintestinal pathogenic Escherichia coli (ExPEC) sequence type ST131 is pandemic, and it is the major contributor to antibiotic resistance in E. coli. Despite its epidemiological superiority, the physiological reasons that decipher its success remain elusive. We aimed to compare the adhesion, invasion and motility potential of ST131 versus other E. coli lineages. METHODOLOGY: In this in vitro comparative study, 14 ESBL-producing ExPEC community-onset bacteremia isolates were chosen from a reported clinical collection (Karfunkel D, Carmeli Y, Chmelnitsky I, Kotlovsky T, Navon-Venezia S...
September 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28552831/extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-healthy-humans-in-mexico-including-subclone-st131-b2-o25-h4-h30-rx
#4
Gerardo Cortés-Cortés, Patricia Lozano-Zarain, Carmen Torres, Carla Andrea Alonso, Ana María Ríos-Torres, Miguel Castañeda, Liliana López-Pliego, Armando Navarro, Rosa Del Carmen Rocha-Gracia
OBJECTIVES: The resistance mechanisms, molecular type and plasmid content of cefotaxime-resistant Escherichia coli isolated from faecal samples of healthy volunteers in Puebla, Mexico, were characterised. METHODS AND RESULTS: Cefotaxime-resistant E. coli were recovered from 11 (18%) of 60 healthy volunteers. The isolates (one per sample) were characterised as multidrug-resistant and phenotypically extended-spectrum β-lactamase (ESBL)-producing strains. Genes encoding resistance to β-lactams (blaCTX-M-15, blaCTX-M-14a, blaCTX-M-14b, blaOXA-1, blaTEM-1b), quinolones [aac(6')-Ib-cr, qnrB19], aminoglycosides [aac(3')-II] and tetracycline [tet(A), tet(B)] were detected among the 11 ESBL-producing E...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28550370/molecular-epidemiology-of-escherichia-coli-sequence-type-131-and-its-h30-h30-rx-subclones-recovered-from-extra-intestinal-infections-first-report-of-oxa-48-producing-st131-clone-from-iran
#5
Z Hojabri, M Mirmohammadkhani, F Kamali, K Ghassemi, S Taghavipour, O Pajand
Multidrug-resistant (MDR) O25b-ST131 clone of Escherichia coli is well established as a significant cause of extra-intestinal infections worldwide. However, there have been no studies about the prevalence of ST131 and its H30/H30Rx subclones from Iran. The prevalence of ST131 was 29.8% among phylogroups B2, D, and F of E.coli isolates recovered from extra-intestinal infections. Fifty-seven (90.4%) and six (9.6%) of isolates belonged to serogroups O25b and O16 respectively, and exhibited high rates of MDR (98...
May 27, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28347271/the-st131-escherichia-coli-h22-subclone-from-human-intestinal-microbiota-comparison-of-genomic-and-phenotypic-traits-with-those-of-the-globally-successful-h30-subclone
#6
Marie-Hélène Nicolas-Chanoine, Marie Petitjean, Azucena Mora, Noémie Mayer, Jean-Philippe Lavigne, Olivier Boulet, Véronique Leflon-Guibout, Jorge Blanco, Didier Hocquet
BACKGROUND: In 2006, we found healthy subjects carrying ST131 Escherichia coli in their intestinal microbiota consisting of two populations: a subdominant population of fluoroquinolone-resistant E. coli belonging to subclone H30 (H30-R or subclade C1), the current worldwide dominant ST131 subclone, and a dominant E. coli population composed of antibiotic-susceptible E. coli belonging to subclone H22 (clade B), the precursor of subclone H30. We sequenced the whole genome of fecal H22 strain S250, compared it to the genomes of ExPEC ST131 H30-Rx strain JJ1886 and commensal ST131 H41 strain SE15, sought the H22-H30 genomic differences in our fecal strains and assessed their phenotypic consequences...
March 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28061803/evaluation-of-mlva-for-epidemiological-typing-and-outbreak-detection-of-esbl-producing-escherichia-coli-in-sweden
#7
Lisa Helldal, Nahid Karami, Christina Welinder-Olsson, Edward R B Moore, Christina Åhren
BACKGROUND: To identify the spread of nosocomial infections and halt outbreak development caused by Escherichia coli that carry multiple antibiotic resistance factors, such as extended-spectrum beta-lactamases (ESBLs) and carbapenemases, is becoming demanding challenges due to the rapid global increase and constant and increasing influx of these bacteria from the community to the hospital setting. Our aim was to assess a reliable and rapid typing protocol for ESBL-E. coli, with the primary focus to screen for possible clonal relatedness between isolates...
January 6, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/27836379/trends-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-sequence-type-131-and-its-h30-subclone-in-a-french-hospital-over-a-15-year-period
#8
Marlène Sauget, Pascal Cholley, Andréa Vannier, Michelle Thouverez, Marie-Hélène Nicolas-Chanoine, Didier Hocquet, Xavier Bertrand
Sequence type 131 (ST131) is a predominant lineage among extraintestinal pathogenic Escherichia coli. It plays a major role in the worldwide dissemination of E. coli producing extended-spectrum β-lactamases (ESBLs). Here we describe the long-term epidemiology of this clonal group in a French university hospital, where the incidence of ESBL-producing E. coli has increased from 0.018 case per 1000 patient-days in the year 2000 to 0.50 case per 1000 patient-days in 2014. The first of the 141 ST131 isolates was recovered in 2006, and the ST131 clonal group accounted for 18...
December 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27798205/community-carriage-of-esbl-producing-escherichia-coli-is-associated-with-strains-of-low-pathogenicity-a-swedish-nationwide-study
#9
Sofia Ny, Sonja Löfmark, Stefan Börjesson, Stina Englund, Maj Ringman, Jakob Bergström, Pontus Nauclér, Christian G Giske, Sara Byfors
OBJECTIVES: Community carriage of ESBL-producing Escherichia coli (EPE) is common worldwide and there is a need to understand the connection between carriage and infection. We compared the molecular characteristics of EPE among Swedish community carriers with those of EPE causing invasive infections. METHODS: We collected 2134 faecal samples from randomly selected Swedish inhabitants and examined them for the presence of EPE. All participating volunteers answered a questionnaire about putative risk factors for EPE carriage...
February 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27436393/the-clinical-impact-of-st131-h30-rx-subclone-in-urinary-tract-infections-due-to-multidrug-resistant-escherichia-coli
#10
Fusun Can, Özlem Kurt-Azap, Pelin İspir, Elif Nurtop, Ceren Seref, İlayda Loçlar, Ozge Nur Aktaş, Yelda Ceren Orhan, Onder Ergonul
In this study, risk factors for ST131 H30 and H30-Rx subclones among urinary tract infections (UTIs) caused by multidrug-resistant (MDR) Escherichia coli were described. Urine samples were collected from consecutive outpatients registered to the outpatient clinics of Başkent University Hospital (Ankara, Turkey) with complaints of acute cystitis in 2011. A total of 107 MDR E. coli isolates were included in the study. Of the 107 isolates studied, 26 (24.3%) were typed as ST131 clone. Extended-spectrum β-lactamase (ESBL)-producers accounted for 59 (55...
March 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27082892/extraintestinal-pathogenic-escherichia-coli-sequence-type-131-h30-r-and-h30-rx-subclones-in-retail-chicken-meat-italy
#11
Arash Ghodousi, Celestino Bonura, Paola Di Carlo, Willem B van Leeuwen, Caterina Mammina
Extraintestinal pathogenic Escherichia coli sequence type 131 (ST131), typically fluoroquinolone-resistant (FQ-R) and/or extended-spectrum β-lactamase (ESBL)-producing, has emerged globally. Among clinical isolates, ST131, primarily its H30-R and H30-Rx subclones, accounts for most antimicrobial-resistant E. coli and is the dominant E. coli strain worldwide. We assessed its prevalence and characteristics among raw chicken meat samples on sale in Palermo, Italy. A collection of 237 fluoroquinolone resistant and ESBL/AmpC producing E...
July 2, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/26940589/biofilm-formation-by-multidrug-resistant-escherichia-coli-st131-is-dependent-on-type-1-fimbriae-and-assay-conditions
#12
Sohinee Sarkar, Dimitrios Vagenas, Mark A Schembri, Makrina Totsika
Escherichia coli sequence type 131 (ST131) has emerged as a pandemic lineage of important multidrug resistant pathogens worldwide. Despite many studies examining the epidemiology of ST131, only a few studies to date have investigated the capacity of ST131 strains to form biofilms. Some of these studies have reported contrasting findings, with no specific ST131 biofilm-promoting factors identified. Here, we examined a diverse collection of ST131 isolates for in vitro biofilm formation in different media and assay conditions, including urine from healthy adult women...
April 2016: Pathogens and Disease
https://www.readbyqxmd.com/read/26926643/modulation-of-membrane-influx-and-efflux-in-escherichia-coli-sequence-type-131-has-an-impact-on-bacterial-motility-biofilm-formation-and-virulence-in-a-caenorhabditis-elegans-model
#13
Alix Pantel, Catherine Dunyach-Remy, Christelle Ngba Essebe, Jennifer Mesureur, Albert Sotto, Jean-Marie Pagès, Marie-Hélène Nicolas-Chanoine, Jean-Philippe Lavigne
Energy-dependent efflux overexpression and altered outer membrane permeability (influx) can promote multidrug resistance (MDR). The present study clarifies the regulatory pathways that control membrane permeability in the pandemic clone Escherichia coli sequence type 131 (ST131) and evaluates the impact of efflux and influx modulations on biofilm formation, motility, and virulence in the Caenorhabditis elegans model. Mutants of two uropathogenic E. coli (UPEC) strains, MECB5 (ST131; H30-Rx) and CFT073 (ST73), as well as a fecal strain, S250 (ST131; H22), were in vitro selected using continuous subculture in subinhibitory concentrations of ertapenem (ETP), chloramphenicol (CMP), and cefoxitin (FOX)...
May 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26795934/molecular-epidemiology-of-bloodstream-associated-e-coli-st131-h30-rx-subclone-infection-in-a-region-with-high-quinolone-resistance
#14
Fusun Can, Ozlem Kurt-Azap, Elif Nurtop, Pelin Ispir, Ceren Seref, Onder Ergonul
Bloodstream infections caused by E. coli ST131 and ST131 H30-Rx subclones have emerged worldwide. This study was carried out to evaluate the prevalence of ST131-Rx subclone and characterize the virulence properties of the Rx among the bloodstream E. coli isolates. A total of 297 non-duplicated E.coli bloodstream isolates were studied. Antibiotic susceptibilities were tested using the disk diffusion method. PCR amplification and sequencing was used to identify ST131 and H30-Rx, the virulence gene, the betalactamase, and virotype...
January 20, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/26686940/antimicrobial-susceptibility-and-emerging-resistance-determinants-blactx-m-rmtb-fosa3-in-clinical-isolates-from-urinary-tract-infections-in-the-bolivian-chaco
#15
Alessandro Bartoloni, Samanta Sennati, Tiziana Di Maggio, Antonia Mantella, Eleonora Riccobono, Marianne Strohmeyer, Carmen Revollo, Ana Liz Villagran, Lucia Pallecchi, Gian Maria Rossolini
BACKGROUND: Bolivia is among the lowest-resourced South American countries, with very few data available on antibiotic resistance in bacterial pathogens. The phenotypic and molecular characterization of bacterial isolates responsible for urinary tract infections (UTIs) in the Bolivian Chaco are reported here. METHODS: All clinical isolates from UTIs collected in the Hospital Basico Villa Montes between June 2010 and January 2014 were analyzed (N=213). Characterization included susceptibility testing, extended-spectrum beta-lactamase (ESBL) detection, identification of relevant resistance determinants (e...
February 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/26407251/emergence-of-antibiotic-resistance-associated-clones-among-escherichia-coli-recovered-from-newborns-with-early-onset-sepsis-and-meningitis-in-the-united-states-2008-2009
#16
Scott J Weissman, Nellie I Hansen, Kristen Zaterka-Baxter, Rosemary D Higgins, Barbara J Stoll
BACKGROUND: Escherichia coli associated with early-onset sepsis (EOS) have historically been antibiotic-susceptible and K1-encapsulated. In the era of emerging antibiotic resistance, however, the clonal makeup of E coli associated with EOS has not been well characterized. METHODS: Escherichia coli isolates were collected from 28 cases of EOS and early-onset meningitis (EOM) from April 2008 through December 2009, during a parent study conducted at National Institute of Child Health and Human Development Neonatal Research Network centers from February 2006 through December 2009...
September 2016: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/26195517/genomic-and-functional-portrait-of-a-highly-virulent-ctx-m-15-producing-h30-rx-subclone-of-escherichia-coli-sequence-type-131
#17
Amit Ranjan, Sabiha Shaik, Arif Hussain, Nishant Nandanwar, Torsten Semmler, Savita Jadhav, Lothar H Wieler, Niyaz Ahmed
Escherichia coli sequence type 131 (ST131) is a pandemic clone associated with multidrug-resistant, extraintestinal infections, attributable to the presence of the CTX-M-15 extended-spectrum β-lactamase gene and mutations entailing fluoroquinolone resistance. Studies on subclones within E. coli ST131 are critically required for targeting and implementation of successful control efforts. Our study comprehensively analyzed the genomic and functional attributes of the H30-Rx subclonal strains NA097 and NA114, belonging to the ST131 lineage...
October 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26100703/variation-in-resistance-traits-phylogenetic-backgrounds-and-virulence-genotypes-among-escherichia-coli-clinical-isolates-from-adjacent-hospital-campuses-serving-distinct-patient-populations
#18
Sarah M Drawz, Stephen Porter, Michael A Kuskowski, Brian Johnston, Connie Clabots, Susan Kline, Patricia Ferrieri, James R Johnson
Escherichia coli sequence type 13 (ST131), an emergent cause of multidrug-resistant extraintestinal infections, has important phylogenetic subsets, notably the H30 and H30Rx subclones, with distinctive resistance profiles and, possibly, clinical associations. To clarify the local prevalence of these ST131 subclones and their associations with antimicrobial resistance, ecological source, and virulence traits, we extensively characterized 233 consecutive E. coli clinical isolates (July and August 2013) from the University of Minnesota Medical Center-Fairview Infectious Diseases and Diagnostic Laboratory, Minneapolis, MN, which serves three adjacent facilities (a children's hospital and low- and high-acuity adult facilities)...
September 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26034762/long-term-care-facilities-are-reservoirs-for-antimicrobial-resistant-sequence-type-131-escherichia-coli
#19
Mary J Burgess, James R Johnson, Stephen B Porter, Brian Johnston, Connie Clabots, Brian D Lahr, James R Uhl, Ritu Banerjee
Background.  Emerging data implicate long-term care facilities (LTCFs) as reservoirs of fluoroquinolone-resistant (FQ-R) Escherichia coli of sequence type 131 (ST131). We screened for ST131 among LTCF residents, characterized isolates molecularly, and identified risk factors for colonization. Methods.  We conducted a cross-sectional study using a single perianal swab or stool sample per resident in 2 LTCFs in Olmsted County, Minnesota, from April to July 2013. Confirmed FQ-R E. coli isolates underwent polymerase chain reaction-based phylotyping, detection of ST131 and its H30 and H30-Rx subclones, extended virulence genotyping, and pulsed-field gel electrophoresis (PFGE) analysis...
January 2015: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/25987621/intensity-and-mechanisms-of-fluoroquinolone-resistance-within-the-h30-and-h30rx-subclones-of-escherichia-coli-sequence-type-131-compared-with-other-fluoroquinolone-resistant-e-coli
#20
James R Johnson, Brian Johnston, Michael A Kuskowski, Evgeni V Sokurenko, Veronika Tchesnokova
The recent expansion of the H30 subclone of Escherichia coli sequence type 131 (ST131) and its CTX-M-15-associated H30Rx subset remains unexplained. Although ST131 H30 typically exhibits fluoroquinolone resistance, so do multiple other E. coli lineages that have not expanded similarly. To determine whether H30 isolates have more intense fluoroquinolone resistance than other fluoroquinolone-resistant E. coli isolates and to identify possible mechanisms, we determined the MICs for four fluoroquinolones (ciprofloxacin, levofloxacin, moxifloxacin, and norfloxacin) among 89 well-characterized, genetically diverse fluoroquinolone-resistant E...
August 2015: Antimicrobial Agents and Chemotherapy
keyword
keyword
22612
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"