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https://www.readbyqxmd.com/read/28350942/prevention-of-surgical-site-infection-in-spine-surgery
#1
Paul A Anderson, Jason W Savage, Alexander R Vaccaro, Kristen Radcliff, Paul M Arnold, Brandon D Lawrence, Mohammed F Shamji
BACKGROUND: Spine surgery is complicated by an incidence of 1% to 9% of surgical site infection (SSI). The most common organisms are gram-positive bacteria and are endogenous, that is are brought to the hospital by the patient. Efforts to improve safety have been focused on reducing SSI using a bundle approach. The bundle approach applies many quality improvement efforts and has been shown to reduce SSI in other surgical procedures. OBJECTIVE: To provide a narrative review of practical solutions to reduce SSI in spine surgery...
March 1, 2017: Neurosurgery
https://www.readbyqxmd.com/read/28350900/gram-positive-bacterial-infections-research-priorities-accomplishments-and-future-directions-of-the-antibacterial-resistance-leadership-group
#2
Sarah B Doernberg, Thomas P Lodise, Joshua T Thaden, Jose M Munita, Sara E Cosgrove, Cesar A Arias, Helen W Boucher, G Ralph Corey, Franklin D Lowy, Barbara Murray, Loren G Miller, Thomas L Holland
Antimicrobial resistance in gram-positive bacteria remains a challenge in infectious diseases. The mission of the Gram-Positive Committee of the Antibacterial Resistance Leadership Group (ARLG) is to advance knowledge in the prevention, management, and treatment of these challenging infections to improve patient outcomes. Our committee has prioritized projects involving methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) due to the scope of the medical threat posed by these pathogens...
March 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28350438/an-acyl-linked-dimer-of-daptomycin-is-strongly-inhibited-by-the-bacterial-cell-wall
#3
Robert M Taylor, Bradley Scott, Scott Taylor, Michael Palmer
The lipopeptide antibiotic daptomycin is active against Gram-positive pathogens. It permeabilizes bacterial cell membranes, which involves the formation of membrane-associated oligomers. We here studied a dimer of daptomycin whose two subunits were linked through a bivalent aliphatic acyl chain. Unexpectedly, the dimer had very low activity on vegetative Staphylococcus aureus and Bacillus subtilis cells. However, activity resembled that of monomeric daptomycin on liposomes and on B. subtilis L-forms. These findings underscore the importance of the bacterial cell wall in daptomycin resistance...
March 28, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28350437/bacteria-activated-theranostic-nanoprobes-against-methicillin-resistant-staphylococcus-aureus-infection
#4
Zhiwei Zhao, Rong Yan, Xuan Yi, Jingling Li, Jiaming Rao, Zhengqing Guo, Yanmei Yang, Weifeng Li, Yong-Qiang Li, Chunying Chen
Despite numerous advanced imaging and sterilization techniques available nowadays, the sensitive in vivo diagnosis and complete elimination of drug-resistant bacterial infections remain to be big challenge. Here we report a strategy to design activatable theranostic nanoprobes against methicillin-resistant Staphylococcus aureus (MRSA) infections. This probe is based on silica nanoparticles coated with vancomycin-modified polyelectrolyte-cypate complexes (SiO2-Cy-Van), which is activated by an interesting phenomenon of bacteria-responsive dissociation of the polyelectrolyte from silica nanoparticles...
March 28, 2017: ACS Nano
https://www.readbyqxmd.com/read/28346800/using-chemoinformatics-bioinformatics-and-bioassay-to-predict-and-explain-the-antibacterial-activity-of-nonantibiotic-food-and-drug-administration-drugs
#5
Nour Aldin Kahlous, Muhammad Al Mohdi Bawarish, Muhammad Arabi Sarhan, Manfred Küpper, Ali Hasaba, Mazen Rajab
Discovering of new and effective antibiotics is a major issue facing scientists today. Luckily, the development of computer science offers new methods to overcome this issue. In this study, a set of computer software was used to predict the antibacterial activity of nonantibiotic Food and Drug Administration (FDA)-approved drugs, and to explain their action by possible binding to well-known bacterial protein targets, along with testing their antibacterial activity against Gram-positive and Gram-negative bacteria...
March 27, 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28346533/correction-a-cross-sectional-study-of-colonization-rates-with-methicillin-resistant-staphylococcus-aureus-mrsa-and-extended-spectrum-beta-lactamase-esbl-and-carbapenemase-producing-enterobacteriaceae-in-four-swiss-refugee-centres
#6
Rein Jan Piso, Roman Käch, Roxana Pop, Daniela Zillig, Urs Schibli, Stefano Bassetti, Dominik M Meinel, Adrian Egli
[This corrects the article DOI: 10.1371/journal.pone.0170251.].
2017: PloS One
https://www.readbyqxmd.com/read/28346009/reactions-with-antisera-and-pathological-effects-of-staphylococcus-aureus-gamma-toxin-in-the-cornea
#7
Michael A Bierdeman, Angela M Torres, Armando R Caballero, Aihua Tang, Richard J O'Callaghan
PURPOSE: This study analyzed the toxicity of purified gamma-toxin from Staphylococcus aureus and the protectiveness of antisera to gamma-toxin in the rabbit cornea. MATERIALS AND METHODS: Gamma-toxin was purified from cultures of alpha-toxin deficient S. aureus strain Newman Δhla. Antisera to native gamma-toxin (Hlg) were produced in rabbits. These antisera and a commercial polyclonal antibody to recombinant HlgB (rHlgB) were analyzed for specificity and toxin neutralization...
March 27, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28345852/layer-number-dependent-antibacterial-and-osteogenic-behaviors-of-graphene-oxide-electrophoretic-deposited-on-titanium
#8
Jiajun Qiu, Hao Geng, Donghui Wang, Shi Qian, Hongqin Zhu, Yuqin Qiao, Wenhao Qian, Xuanyong Liu
Graphene oxide has attracted widespread attentions in the biomedical fields due to its excellent biocompatibility. Herein we investigated the layer-number dependent antibacterial and osteogenic behaviors of graphene oxide in biointerfaces. Graphene oxide with different layer numbers was deposited on the titanium surfaces by cathodal electrophoretic deposition with varied deposition voltages. The initial cell adhesion and spreading, cell proliferation and osteogenic differentiation were observed from all the samples using rat bone mesenchymal stem cells...
March 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28345028/self-powered-integrated-microfluidic-point-of-care-low-cost-enabling-simple-chip
#9
Erh-Chia Yeh, Chi-Cheng Fu, Lucy Hu, Rohan Thakur, Jeffrey Feng, Luke P Lee
Portable, low-cost, and quantitative nucleic acid detection is desirable for point-of-care diagnostics; however, current polymerase chain reaction testing often requires time-consuming multiple steps and costly equipment. We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps. First, we prepatterned the amplification initiator [magnesium acetate (MgOAc)] on the chip to enable digital nucleic acid amplification...
March 2017: Science Advances
https://www.readbyqxmd.com/read/28344976/a-metataxonomic-approach-could-be-considered-for-cattle-clinical-mastitis-diagnostics
#10
Joanne W H Oultram, Erika K Ganda, Sarah C Boulding, Rodrigo C Bicalho, Georgios Oikonomou
Mastitis is one of the most costly diseases affecting the dairy industry, and identification of the causative microorganism(s) is essential. Here, we report the use of next-generation sequencing of bacterial 16S rRNA genes for clinical mastitis diagnosis. We used 65 paired milk samples, collected from the mastitic and a contralateral healthy quarter of mastitic dairy cattle to evaluate the technique as a potential alternative to bacterial culture or targeted PCR. One large commercial dairy farm was used, with one trained veterinarian collecting the milk samples...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28344783/retrospective-report-of-antimicrobial-susceptibility-observed-in-bacterial-pathogens-isolated-from-ocular-samples-at-mount-sinai-hospital-2010-to-2015
#11
Marko Oydanich, Tanis C Dingle, Camille L Hamula, Claudia Ghisa, Penny Asbell
BACKGROUND: Antimicrobial resistance has emerged as a major threat to global public health. Thus, the surveillance of changes in antimicrobial resistance in local and global settings is a paramount necessity. While many studies have tracked antimicrobial resistance, only a small percentage surveyed ocular isolates. The purpose of this study was to report the in vitro susceptibility of bacterial pathogens isolated from ocular samples in New York, NY from 2010 to 2015. METHODS: A retrospective review of ocular isolates was conducted...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28344478/synthesis-and-antimicrobial-properties-of-4-acylaminobenzenethiosulfoacid-s-esters
#12
Vira Lubenets, Sofiya Vasylyuk, Nataliya Monka, Khrystyna Bolibrukh, Olena Komarovska-Porokhnyavets, Diana Baranovych, Rostyslav Musyanovych, Ewa Zaczynska, Anna Czarny, Urszula Nawrot, Volodymyr Novikov
A series of esters of 4-acetyl, 4-trifluoroacetyl- and 4-(3-chloropropionyl)aminobenzenethiosulfoacids (twenty-four compounds) were synthesized and characterized by elemental analysis, (1)H NMR and IR spectroscopy. The antibacterial activity of the novel candidates has been screened using the agar diffusion or serial dilution methods against representative Gram-positive (Staphylococcus aureus, Bacillus subtilis, Bacillus mesentericus, Mycobacterium sp., Mycobacterium luteum), Gram-negative (Aeromonas sp., Burkholderia cepacia, Alcaligenes faecalis, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris) bacteria and fungi (Candida albicans, Candida tenuis, Candida glabrata, Verticillium dahliae, Trichophyton gypseum, Aspergillus niger, Aspergillus fumigatus, Penicillium chrysogenum)...
February 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28344408/potential-antibacterial-activity-of-some-saudi-arabia-honey
#13
Ahmed G Hegazi, Faiz M Al Guthami, Ahmed F M Al Gethami, Fyrouz M Abd Allah, Ashraf A Saleh, Ehab A Fouad
AIM: The aim of this study was to investigate the potential antibacterial activity of some Saudi Arabia honey against selected bacterial strains of medical importance. MATERIALS AND METHODS: A total of 10 Saudi Arabia honey used to evaluate their antimicrobial activity against some antibiotic-resistant pathogenic bacterial strains. The bacterial strains were Staphylococcus aureus, Streptococcus pyogenes, Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28344406/attribution-of-antibacterial-and-antioxidant-activity-of-cassia-tora-extract-toward-its-growth-promoting-effect-in-broiler-birds
#14
Jyoti Sahu, K M Koley, B D Sahu
AIM: The study was conducted to evaluate the attribution of antibacterial and antioxidant activity of methanolic extract of Cassia tora toward its growth promoting effect in broiler birds. MATERIALS AND METHODS: A limit test was conducted for C. tora extract in Wistar albino rats. Phytochemical screening of methanolic extract of leaves of C. tora was carried out. In-vitro antibacterial activity was measured by disc diffusion method. 1-day-old Ven Cobb broiler birds (n=90) were randomly allocated into three groups consisting of three replicates with 10 birds in each group...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28344399/extraction-of-chitosan-and-its-oligomers-from-shrimp-shell-waste-their-characterization-and-antimicrobial-effect
#15
Tarun Kumar Varun, Swaraj Senani, Natasha Jayapal, Jayaram Chikkerur, Sohini Roy, Vijay Bhasker Tekulapally, Mayank Gautam, Narender Kumar
AIM: The present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by Fourier transform infrared (FT-IR) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid. MATERIALS AND METHODS: Chitosan was extracted from the shrimp shell waste by the chemical method and characterized by FT-IR. Chitooligomers were produced by depolymerising chitosan using nitrous acid, and the chitooligomers were tested for antimicrobial effect against four gut pathogenic organisms, i...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28344030/antibacterial-activity-of-medicinal-plants-from-the-physicians-of-myddvai-a-13-th-century-welsh-medical-manuscript
#16
Charles Wagner, Jillian De Gezelle, Maureen Robertson, Keith Robertson, Mickey Wilson, Slavko Komarnytsky
ETHNOPHARMACOLOGICAL RELEVANCE: Antimicrobial drug resistance is a growing threat to global public health. Historical records and herbal texts relating to traditional Celtic medicine indicate an extensive pharmacopeia of plants for treating infections likely caused by microbes. However, a major barrier for successful integration of these remedies into mainstream practice is the current lack of accurate interpretation and scientific validation. MATERIALS AND METHODS: We applied Mobile Discovery approach to the Isle of Arran, Scotland, for in situ targeted screening of 83 out of 138 plants identified in Meddygion Myddvai (a 13(th) century Welsh manuscript) to treat conditions related to microbial infections, and an additional 18 plants from modern ethnobotanical knowledge on the island...
March 24, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28343858/-inhibitory-capacity-of-lactobacillus-spp-against-pathogens-involved-in-foodborne-diseases
#17
María J Ruiz, Rocío Colello, Nora L Padola, Analía I Etcheverría
The genus Lactobacillus daily generates a growing interest among microbiologists and technologists, who try to discover new biotechnological applications and probiotic properties. The main goal of this study was to evaluate the inhibitory capacity of Lactobacillus spp. against pathogens (Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus) involved in foodborne diseases. For this purpose, samples were collected at different stages of the pork production chain. Seventy eight bacterial strains were isolated...
March 23, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28343857/staphylococcus-aureus-nasal-carriage-in-health-care-workers-first-report-from-a-major-public-hospital-in-argentina
#18
Carina Andrea Boncompain, Cristian Alejandro Suárez, Héctor Ricardo Morbidoni
Staphylococcus aureus causes numerous mild to severe infections in humans, both in health facilities and in the community. Patients and health care workers (HCWs) may disseminate strains during regular medical examinations or hospitalization. The aim of this study was to determine the nasal carriage rate of methicillin-susceptible and methicillin-resistant S. aureus among health care workers at Hospital Provincial del Centenario, a public general hospital in Rosario, Argentina. A transversal study was conducted on 320 health care workers...
March 24, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28343752/efficacy-of-tedizolid-against-methicillin-resistant-staphylococcus-aureus-and-peptostreptococcus-anaerobius-in-thigh-mixed-infection-mouse-model
#19
Yuka Yamagishi, Hiroshige Mikamo, Hideo Kato, Naoya Nishiyama, Nobuhiro Asai, Yusuke Koizumi, Daisuke Sakanashi, Hiroyuki Suematsu, Katsuhiko Matsuura, Mao Hagihara
OBJECTIVE: The purpose of this study is to compare the antimicrobial activity of human simulated exposures of tedizolid 200 mg daily, and linezolid 600 mg every 12 h for the treatment of complicated skin and skin structure infection (cSSSI) caused by MRSA and Peptostreptococcus anaerobius in both the neutropenic mice thigh mixed-infection models. MATERIAL AND METHOD: Tedizolid phosphate and linezolid were used for all in vivo testing. A total of one MRSA and two P...
March 23, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28343639/the-benefits-of-a-preoperative-mrsa-swabbing-protocol
#20
Hope Herd
Infection control practices pose a challenge to nursing care in general, but can have a huge negative impact on the perioperative process. Prior to July of 2012, our institution did not perform routine methacillin resistant staphylococcus aureus (MRSA) screening on preoperative patients with a prior history of MRSA. This resulted in patients remaining in isolation throughout their entire perioperative course. Screening for MRSA was delayed until the patient arrived in the medical surgical unit. Many of these patients were later found to have negative nasal swabs...
April 2017: Journal of Perianesthesia Nursing: Official Journal of the American Society of PeriAnesthesia Nurses
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