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https://www.readbyqxmd.com/read/28825446/application-of-maldi-tof-ms-fingerprinting-as-a-quick-tool-for-identification-and-clustering-of-foodborne-pathogens-isolated-from-food-products
#1
Ayman Elbehiry, Eman Marzouk, Mohamed Hamada, Musaad Al-Dubaib, Essam Alyamani, Ihab M Moussa
Foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. Since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in Al-Qassim region, Kingdom of Saudi Arabia (KSA) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Sixty-nine bacterial and thirty-two fungal isolates isolated from 80 food samples were used in this study...
August 21, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28824581/stress-driven-discovery-of-novel-cryptic-antibiotics-from-a-marine-fungus-penicillium-sp-bb1122
#2
Bibi N Auckloo, Chengqian Pan, Najeeb Akhter, Bin Wu, Xiaodan Wu, Shan He
Standard laboratory cultures have long been known to hinder activation of specific gene clusters which in turn hamper production of secondary metabolites with unique properties due to lack of innovation or the inability to trigger cryptic gene clusters' expression. Due to challenges related to the avoidance of the isolation of replicated metabolites, resistance-developing pathogens are to be addressed by the scientific community worldwide in order to progress with novel and potent compounds which could further be developed in the future for pharmaceutical usage...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28824577/identification-of-variable-traits-among-the-methicillin-resistant-and-sensitive-coagulase-negative-staphylococci-in-milk-samples-from-mastitic-cows-in-india
#3
Sudipta Mahato, Hiral U Mistry, Shalini Chakraborty, Paresh Sharma, R Saravanan, Vasundhra Bhandari
Methicillin resistant Staphylococcus aureus causing bovine mastitis has been very well investigated worldwide. However, there are only limited reports on the characterization of methicillin resistant and sensitive coagulase negative staphylococci (CoNS) across the globe. Hence, in the present study, we aim to determine the phenotypic traits based on antimicrobial susceptibility profile and genotypic characterization by verifying the presence of resistance determinants, virulence and toxin genes present in the CoNS causing clinical mastitis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28824564/identification-of-major-sequence-types-among-multidrug-resistant-staphylococcus-epidermidis-strains-isolated-from-infected-eyes-and-healthy-conjunctiva
#4
Smrutiti Jena, Sasmita Panda, Kinshuk C Nayak, Durg V Singh
We examined the presence of virulence and antibiotic resistance genes, SCCmec types and determined the genomic diversity among ocular S. epidermidis isolates (patients-23, healthy controls-29). PCR determined the presence of antibiotic resistance genes, virulence genes and SCCmec types among all isolates. MLST and PFGE determined the genomic relatedness among them. All isolates of S. epidermidis showed resistance to at least one class of antibiotics of which 48 isolates were multidrug resistant and carried ARGs...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28824556/pathogenic-mechanisms-and-host-interactions-in-staphylococcus-epidermidis-device-related-infection
#5
REVIEW
Marina Sabaté Brescó, Llinos G Harris, Keith Thompson, Barbara Stanic, Mario Morgenstern, Liam O'Mahony, R Geoff Richards, T Fintan Moriarty
Staphylococcus epidermidis is a permanent member of the normal human microbiota, commonly found on skin and mucous membranes. By adhering to tissue surface moieties of the host via specific adhesins, S. epidermidis is capable of establishing a lifelong commensal relationship with humans that begins early in life. In its role as a commensal organism, S. epidermidis is thought to provide benefits to human host, including out-competing more virulent pathogens. However, largely due to its capacity to form biofilm on implanted foreign bodies, S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28823969/rapid-classification-of-heavy-metal-exposed-freshwater-bacteria-by-infrared-spectroscopy-coupled-with-chemometrics-using-supervised-method
#6
Rafig Gurbanov, Ayse Gul Gozen, Feride Severcan
Rapid, cost-effective, sensitive and accurate methodologies to classify bacteria are still in the process of development. The major drawbacks of standard microbiological, molecular and immunological techniques call for the possible usage of infrared (IR) spectroscopy based supervised chemometric techniques. Previous applications of IR based chemometric methods have demonstrated outstanding findings in the classification of bacteria. Therefore, we have exploited an IR spectroscopy based chemometrics using supervised method namely Soft Independent Modeling of Class Analogy (SIMCA) technique for the first time to classify heavy metal-exposed bacteria to be used in the selection of suitable bacteria to evaluate their potential for environmental cleanup applications...
August 15, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28823093/candida-albicans-staphylococcus-aureus-dual-species-biofilm-as-a-target-for-the-combination-of-essential-oils-and-fluconazole-or-mupirocin
#7
Aleksandra Budzyńska, Sylwia Różalska, Beata Sadowska, Barbara Różalska
The effectiveness of essential oils (EOs), fluconazole (FLU) and mupirocin (MUP) used alone or in combination against mono-species and mixed Candida albicans/Staphylococcus aureus biofilms was examined. An experimentally established dual-species biofilm model, verified by fluorescence microscopy and viable cell counting, was used. Selected commercial EOs were tested: geranium, citronella and clove oils, which have been chemically characterized and found to differ in the content of the main components (qualitative and quantitative)...
August 19, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28822873/surface-modification-of-an-intraocular-lens-material-by-plasma-assisted-grafting-with-2-hydroxyethyl-methacrylate-hema-for-controlled-release-of-moxifloxacin
#8
A Paula Vieira, Andreia F R Pimenta, Diana Silva, M Helena Gil, Patrícia Alves, Patrícia Coimbra, José L G C Mata, Dimitriya Bozukova, Tiago R Correia, Ilídio J Correia, A Paula Serro, A Jorge Guiomar
Endophthalmitis, an inflammation of the eye due to perioperative infection, may occur after cataract surgery. Intraocular lenses (IOLs) loaded with an antibiotic have been proposed as an alternative to the conventional postoperative endophthalmitis prophylaxis, since the antibiotic is delivered directly to the target site. In this work, an IOL-based antibiotic releasing system was prepared from a copolymer used in the production of IOLs and a fluoroquinolone used in endophthalmitis prophylaxis (moxifloxacin, MFX)...
August 16, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28822698/staphylococcus-aureus-from-atopic-dermatitis-skin-alters-cytokine-production-triggered-by-monocyte-derived-langerhans-cell
#9
Kazumasa Iwamoto, Masaya Moriwaki, Yoshie Niitsu, Masachika Saino, Shunsuke Takahagi, Junzo Hisatsune, Motoyuki Sugai, Michihiro Hide
BACKGROUND: Atopic dermatitis (AD) is one of the most common chronic inflammatory skin diseases. The skin of patients with AD presents as a disbalance of the microbiome with a strong colonization by Staphylococcus aureus, which positively correlates with the severity of the disease. However, the effect of colonized S. aureus on the skin immune system has not been fully elucidated. OBJECTIVE: The aim of this study is to explore whether S. aureus isolated from AD skin is able to skew T cell responses via Langerhans cells (LC) as compared to a standard strain of S...
August 5, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28822595/microbiological-assessment-of-honey-in-m%C3%A3-xico
#10
Carlos Ramón Vázquez-Quiñones, Rúben Moreno-Terrazas, Iván Natividad-Bonifacio, Elsa Irma Quiñones-Ramírez, Carlos Vázquez-Salinas
Honey is a product used as a natural sweetener and in several regions of Mexico and other countries it is also used as a therapeutic agent. Microbiological contamination of honey can occur during its extraction and handling. Due to the use and consumption of honey we highlighted here the importance of the assessment of its microbiological quality. One thousand nine hundred twenty samples obtained from 8 honey-producing states from Mexico were analyzed. From these samples, 40.5% (777/1920) did not comply with the NMX-036-NORMEX-2006 specification...
August 16, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28822551/disruption-of-staphylococcus-aureus-biofilms-using-rhamnolipid-biosurfactants
#11
S S E Silva, J W P Carvalho, C P Aires, M Nitschke
Staphylococcus aureus is an important pathogen that has shown ability to establish biofilm communities that can represent a source of contamination and resistance in food processing. Rhamnolipids (RL) have attracted attention as candidates to replace synthetic surfactants, exhibiting high surface activity combined with its microbial origin, biodegradability, and low toxicity. In this work, an RL biosurfactant was evaluated regarding its ability to disrupt or remove S. aureus biofilms established on polystyrene plates using nutrient broth and skim milk as the growth media...
August 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28822159/-effect-of-sodium-houttuyfonate-in-combination-with-erythromycin-on-luxs-agr-rna%C3%A2-of-staphylococus-epidermidis
#12
Gan-Fei Xu, Jing-Jing Wang, Da-Qiang Wu, Yan Guan
Quorum sensing of bacteria and its specific gene expression regulation have a very important role in bacterial biofilm formation. LuxS and agr are the key regulatory genes in quorum sensing of Staphylococcus epidermidis, and RNA Ⅲ is the effector molecule of agr system. In order to evaluate the effects of sodium houttuyfonate in combination with erythromycin on the transcription level of S. epidermidis, serial dilution method was used to determine the MIC of sodium houttuyfonate, erythromycin and vancomycin on S...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822150/-antimicrobial-and-anti-tumor-activities-of-endophytes-from-lycium-barbarum-of-ningxia
#13
Jin-Xia Dai, Xiao-Ning Du
The antimicrobial activity and cytotoxicity in vitro of the fermentation broth of 10 endophytic strains isolated from Lycium barbarum were determined to screen high activity endophytic strains. Sequences analysis of ITS and 16S rDNA was used for molecular identification of the strains. The results showed that 5 endophytic fungi had no inhibitory activity against the tested pathogens. Endophytic actinomycete strain AL6 had a certain inhibitory effect on 3 kinds of pathogenic fungi, and strain AL5 only had strong inhibitory activity against Staphylococcus aureus...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28821946/quantitative-expression-analysis-of-spa-fnba-and-rsp-genes-in-staphylococcus-aureus-actively-associated-in-the-formation-of-biofilms
#14
Sthanikam Yeswanth, Abhijit Chaudhury, Potukuchi Venkata Gurunadha Krishna Sarma
In Staphylococcus aureus, adherence and secretory proteins play chief role in the formation of biofilms. This mode of growth exhibits resistance to a variety of antibiotics and spreads its infections. In the present study, secretary and adherence proteins, Protein-A, Fibronectin-binding protein-A (FnbA) and Rsp (a transcription regulator encoding proteolytic property) expression levels were evaluated at different stages of growth in S. aureus ATCC12600 a drug-sensitive strain and multidrug-resistant strains of S...
August 18, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28821865/identification-of-bacterial-biofilm-and-the-staphylococcus-aureus-derived-protease-staphopain-on-the-skin-surface-of-patients-with-atopic-dermatitis
#15
Andreas Sonesson, Kornelia Przybyszewska, Sigrid Eriksson, Matthias Mörgelin, Sven Kjellström, Julia Davies, Jan Potempa, Artur Schmidtchen
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by an impaired epidermal barrier, dysregulation of innate and adaptive immunity, and a high susceptibility to bacterial colonization and infection. In the present study, bacterial biofilm was visualized by electron microscopy at the surface of AD skin. Correspondingly, Staphylococcus aureus (S. aureus) isolates from lesional skin of patients with AD, produced a substantial amount of biofilm in vitro. S. aureus biofilms showed less susceptibility to killing by the antimicrobial peptide LL-37 when compared with results obtained using planktonic cells...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821610/a-screen-for-kinase-inhibitors-identifies-antimicrobial-imidazopyridine-aminofurazans-as-specific-inhibitors-of-the-listeria-monocytogenes-pasta-kinase-prka
#16
Adam J Schaenzer, Nathan Wlodarchak, David H Drewry, William J Zuercher, Warren E Rose, Rob Striker, John-Demian Sauer
Bacterial signaling systems such as protein kinases and quorum sensing have become increasingly attractive targets for the development of novel antimicrobial agents in a time of rising antibiotic resistance. The family of bacterial Penicillin-binding-protein And Serine/Threonine kinase-Associated (PASTA) kinases is of particular interest due to the role of these kinases in regulating resistance to β-lactam antibiotics. As such, small-molecule kinase inhibitors that target PASTA kinases may prove beneficial as treatments adjunctive to β-lactam therapy...
August 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28821529/antibiotic-susceptibility-profiles-of-ocular-and-nasal-flora-in-patients-undergoing-cataract-surgery-in-taiwan-an-observational-and-cross-sectional-study
#17
Yun-Hsuan Lin, Yu-Chuan Kang, Chiun-Ho Hou, Yhu-Chering Huang, Chih-Jung Chen, Jwu-Ching Shu, Pang-Hsin Hsieh, Ching-Hsi Hsiao
OBJECTIVE: To investigate the conjunctival and nasal flora and the antibiotic susceptibility profiles of isolates from patients undergoing cataract surgery. DESIGN: Observational and cross-sectional study. SETTING: A single-centre study in Taiwan. PARTICIPANTS: 128 consecutive patients precataract surgery. PRIMARY AND SECONDARY OUTCOME MEASURES METHODS: Conjunctival and nasal cultures were prospectively obtained from 128 patients on the day of cataract surgery before instillation of ophthalmic solutions in our hospital...
August 18, 2017: BMJ Open
https://www.readbyqxmd.com/read/28821119/tailoring-of-temperature-and-ph-responsive-cotton-fabric-with-antimicrobial-activity-effect-of-the-concentration-of-a-bio-barrier-forming-agent
#18
Danaja Štular, Ivan Jerman, Barbara Simončič, Brigita Tomšič
A stimuli-responsive cotton fabric was designed using temperature and pH-responsive poly-N-isopropylacrylamide (poly-NiPAAm) and chitosan (PNCS) microgel as a carrier of antimicrobially active 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride (Si-QAC), which forms a bio-barrier on the fibre surface. The influence of Si-QAC on the moisture management and thermoregulation abilities of the PNCS microgel was investigated. Using a pad-dry cure method, Si-QAC was applied to a 100% cotton fabric model in concentrations ranging from 0...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821033/novel-nitroaniline-cellulose-nanohybrids-nitro-radical-photo-release-and-its-antibacterial-action
#19
Prashant Chauhan, Ning Yan
In this study, carboxylated nanocrystalline cellulose is surface functionalized by a chromophoric nitro radical (NO) photodonor via covalent bonding. The resulting new photoactive nanohybrid material displays an effective photogeneration of NO radicals upon illumination by light. The release of NO radicals has been chemically detected by various spectroscopic techniques. The bactericidal activity of the novel nanohybrid is effectively demonstrated against both Gram positive (Staphylococcus aureus) and Gram negative (Pseudomonas aeruginosa) bacteria with light...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821030/preparation-and-characterization-of-a-photocatalytic-antibacterial-material-graphene-oxide-tio2-bacterial-cellulose-nanocomposite
#20
Ling-Pu Liu, Xiao-Ning Yang, Li Ye, Dong-Dong Xue, Miao Liu, Shi-Ru Jia, Ying Hou, Li-Qiang Chu, Cheng Zhong
In this study, bacterial cellulose (BC) was used as a matrix to synthesize graphene oxide/Titanium dioxide (GOTiO2)-based hybrid materials. It was indicated by X-ray diffraction and selected area electron diffraction that the crystal structure of GOTiO2 was a mixed phase containing anatase and rutile. TiO2 nanoparticles were of 10-30nm diameters and densely anchored on graphene oxide sheets. Superior photocatalytic performance of the GOTiO2 was achieved under near UV excitation. The photocatalytic efficiency was optimized through controlling an appropriate calcined temperature...
October 15, 2017: Carbohydrate Polymers
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