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Anjali Chelliah, Niti Dham, Lowell H Frank, Mary Donofrio, Anita Krishnan
OBJECTIVES: Alterations in myocardial strain can identify cardiac dysfunction and can be measured in the mid-gestation fetus. This study evaluates feasibility of strain measurements in early fetuses and establishes normal early fetal strain values. METHODS: Normal fetal echocardiograms were reviewed for image adequacy for strain measurements in 12- to 14-week gestation fetuses. Two readers performed independent strain measurements. Values were compared with data from 40 normal second trimester fetuses...
May 2016: Prenatal Diagnosis
Reynier Suardíaz, Pablo G Jambrina, Laura Masgrau, Àngels González-Lafont, Edina Rosta, José M Lluch
Lipoxygenases (LOXs) are a family of enzymes involved in the biosynthesis of several lipid mediators. In the case of human 15-LOX, the 15-LOX-1 and 15-LOX-2 isoforms show slightly different reaction regiospecificity and substrate specificity, indicating that substrate binding and recognition may be different, a fact that could be related to their different biological role. Here, we have used long molecular dynamics simulations, QM(DFT)/MM potential energy and free energy calculations (using the newly developed DHAM method), to investigate the binding mode of the arachidonic acid (AA) substrate into 15-LOX-2 and the rate-limiting hydrogen-abstraction reaction 15-LOX-2 catalyzes...
April 12, 2016: Journal of Chemical Theory and Computation
M Gholipourmalekabadi, M Sameni, Dina Radenkovic, M Mozafari, M Mossahebi-Mohammadi, A Seifalian
OBJECTIVES: Human amniotic membrane (HAM) has been widely used in soft tissue engineering both in its fresh form and decellularized; its efficiency to aid treatment of burn injuries is well known. On the other hand, it has been reported clinically by several studies that human adipose-derived stem cells (hADSC) are a promising cell source for cell therapy for burns. Recently, we have reported a new technique for decellularization of HAM. In this study, potential of prepared decellularized HAM (dHAM) as a viable support for proliferation and delivery of hADSC was investigated...
February 2016: Cell Proliferation
César Aguilar, Noemí Flores, Fernando Riveros-McKay, Diana Sahonero-Canavesi, Susy Beatriz Carmona, Otto Geiger, Adelfo Escalante, Francisco Bolívar
BACKGROUND: As a metabolic engineering tool, an adaptive laboratory evolution (ALE) experiment was performed to increase the specific growth rate (µ) in an Escherichia coli strain lacking PTS, originally engineered to increase the availability of intracellular phosphoenolpyruvate and redirect to the aromatic biosynthesis pathway. As result, several evolved strains increased their growth fitness on glucose as the only carbon source. Two of these clones isolated at 120 and 200 h during the experiment, increased their μ by 338 and 373 %, respectively, compared to the predecessor PB11 strain...
2015: Microbial Cell Factories
Edina Rosta, Gerhard Hummer
The weighted histogram analysis method (WHAM) is widely used to obtain accurate free energies from biased molecular simulations. However, WHAM free energies can exhibit significant errors if some of the biasing windows are not fully equilibrated. To account for the lack of full equilibration, we develop the dynamic histogram analysis method (DHAM). DHAM uses a global Markov state model to obtain the free energy along the reaction coordinate. A maximum likelihood estimate of the Markov transition matrix is constructed by joint unbiasing of the transition counts from multiple umbrella-sampling simulations along discretized reaction coordinates...
January 13, 2015: Journal of Chemical Theory and Computation
Pallavi Dham, Amii Larsen, Michael Baigent
OBJECTIVES: This case report discusses the response to exposure and response prevention therapy in a patient with problem gambling and comorbid early dementia. It further discusses neurobiology of exposure therapy and its application in patients with mild cognitive impairment. CONCLUSION: Studies show good response to the use of exposure therapy in patients with cognitive impairment and further studies are needed to study the neurobiological changes and response to the therapy in patients with cognitive impairment...
October 2015: Australasian Psychiatry: Bulletin of Royal Australian and New Zealand College of Psychiatrists
Umer Akbar, Bhavpreet Dham, Ying He, Nawaz Hack, Samuel Wu, Michelle Troche, Patrick Tighe, Eugene Nelson, Joseph H Friedman, Michael S Okun
INTRODUCTION: Careful examination of long-term analyses and trends is essential in understanding the medico-economic burden of this common complication. We sought to describe the long-term (32-year) trends of incidence and mortality in PD patients hospitalized with aspiration pneumonia (AsPNA). METHODS: Incidence and mortality of AsPNA in hospitalized PD versus non-PD patients was assessed by logistic regression analysis applied to a national database between the years 1979 and 2010...
September 2015: Parkinsonism & related Disorders
Peina Shi, Mengna Gao, Qiuxia Shen, Lei Hou, Yabin Zhu, Jun Wang
Meshes play important roles to repair human tissue defect. In this work, human amniotic membrane (HAM) was decellularized and explored the efficacy as an implantable biological mesh. Surfactant, hypertonic saline, lipase and DNAase were used individually or collectively to remove all cell components and remain the extracellular matrix. Results of H&E and DAPI staining demonstrated that the method of surfactant and lipase combining with DNAase is the most effective treatment for HAM decellularization. Primary smooth muscle cells were seeded to evaluate the decellularized HAM's (dHAM) in vitro cytocompatibility...
September 2015: Materials Science & Engineering. C, Materials for Biological Applications
Ofer Schiller, Niti Dham, E Anne Greene, Deneen M Heath, Mark E Alexander, Charles I Berul
INTRODUCTION: Cardiac resynchronization therapy (CRT) is an effective device-based intervention for adults with heart failure (HF) with specific indications, based on large, multicenter randomized clinical trials. The criteria for CRT in adult HF include significant symptoms, ventricular systolic dysfunction, prolonged QRS duration, and left bundle branch block (LBBB) pattern on electrocardiogram (ECG). Despite having less data, CRT is also being widely utilized in children with HF. The shortage of evidence-based CRT criteria in pediatrics prompted us to review a cohort of children with dilated cardiomyopathy and evaluate their potential eligibility for CRT using the traditional adult criteria...
August 2015: Journal of Cardiovascular Electrophysiology
Vipul R Patel, Srinivas Samavedi, Anthony S Bates, Anup Kumar, Rafael Coelho, Bernardo Rocco, Kenneth Palmer
We present a propensity-matched analysis of patients undergoing placement of dehydrated human amnion/chorion membrane (dHACM) around the neurovascular bundle (NVB) during nerve-sparing (NS) robot-assisted laparoscopic prostatectomy (RARP). From March 2013 to July 2014, 58 patients who were preoperatively potent (Sexual Health Inventory for Men [SHIM] score >19) and continent (no pads) underwent full NS RARP. Postoperative outcomes were analyzed between propensity-matched graft and no-graft groups, including time to return to continence, potency, and biochemical recurrence...
June 2015: European Urology
Shagun Sachdeva, Xiaoyan Song, Niti Dham, Deneen M Heath, Roberta L DeBiasi
Myocarditis causes significant morbidity and mortality in pediatric patients, with potential adverse outcomes including heart failure, transplantation requirement, and/or death. The objective of this study was to determine predictors of early and late poor outcomes, defined as requirement for extracorporeal membrane oxygenation, ventricular assist device, transplantation, or death in pediatric myocarditis patients. A retrospective cohort study was conducted to evaluate pediatric myocarditis presenting over a 5-year period at a pediatric institution...
February 15, 2015: American Journal of Cardiology
Ashok Gunawardene, Mohan Singh, Afzal Mohammed, Edward Harper, Dham Mobarak
No abstract text is available yet for this article.
October 2013: International Journal of Surgery
Bhavpreet S Dham, Krystal Hunter, Fred Rincon
OBJECTIVE: To summarize trends in status epilepticus (SE) in the United States by age, race, sex, admission source, disposition, incidence rates, and mortality. METHODS: Data from US National Hospital Discharge Survey were used from 1979 to 2010 to identify discharges with SE and common etiologies and complications of SE using International Classification of Diseases, 9th Revision, Clinical Modifications codes. Temporal trends in the incidence and in-hospital mortality of SE were examined with respect to age, sex, and race...
June 2014: Neurocritical Care
Manuel Arteta, Andrew Campbell, Mehdi Nouraie, Sohail Rana, Onyinye C Onyekwere, Gregory Ensing, Craig Sable, Niti Dham, Deepika Darbari, Lori Luchtman-Jones, Gregory J Kato, Mark T Gladwin, Oswaldo L Castro, Caterina P Minniti, Victor R Gordeuk
Obstructive and restrictive pulmonary changes develop in children with sickle cell disease, but reports conflict as to the type of change that predominates. We prospectively performed spirometry, plethysmography, and lung diffusing capacity in 146 children aged 7 to 20 years with hemoglobin SS or Sβ(0)-thalassemia. Nineteen percent of the patients had obstructive physiology as defined according to guidelines of the American Thoracic Society. In addition, 9% had restrictive physiology and 11% had abnormal but not categorized physiology...
April 2014: Journal of Pediatric Hematology/oncology
C M Zelen
OBJECTIVE: To evaluate healing of chronic diabetic foot ulcers (DFUs) with use of dehydrated human amniotic membrane (dHAM) in patients failing under standard of care treatment. METHOD: The study population was identified from patients with chronic DFUs enrolled in a randomised trial comparing standard care with standard care with the addition of dHAM. The present study included patients that failed to heal (defined as < 50% decrease in wound size after 6 weeks, or not completely healed by 12 weeks of treatment) with standard care...
July 2013: Journal of Wound Care
Pallavi Dham, Jacob Alexander
INTRODUCTION: The thalamus is increasingly gaining importance in psychiatric disorders. There are case reports in the literature of neuropsychiatric symptoms associated with thalamic infarcts. The present report elucidates the complexities of linking neuropsychiatric symptoms to a benign thalamic brain lesion, and its impact on management. CASE PRESENTATION: We present the case of a Caucasian man in his early 30s, who presented with a difficult to treat bipolar illness and coexisting thalamic lesion...
2013: Journal of Medical Case Reports
Ting Zhang, Gary Hin-Fai Yam, Andri K Riau, Rebekah Poh, John C Allen, Gary S Peh, Roger W Beuerman, Donald T Tan, Jodhbir S Mehta
PURPOSE: We characterized the de-epithelialized human amniotic membrane (HAM), and compared cell attachment and proliferation efficiencies. METHODS: HAM was de-epithelialized by 20% ethanol (AHAM), 1.2 U/mL Dispase (DHAM), 0.02% EDTA (EHAM), 0.25% trypsin-EDTA (THAM), and 5 M urea (UHAM), respectively, followed by gentle scraping with a #15 blade. Surface topology, extracellular matrix (ECM), and growth factor content were characterized and compared to intact HAM by electron microscopies (EM), atomic force microscopy (AFM), immunohistochemistry, and Western blotting...
April 2013: Investigative Ophthalmology & Visual Science
Rabindra Paul, Caterina P Minniti, Mehdi Nouraie, Lori Luchtman-Jones, Andrew Campbell, Sohail Rana, Onyinye Onyekwere, Deepika S Darbari, Olaid Ajayi, Manuel Arteta, Gregory Ensing, Craig Sable, Niti Dham, Gregory J Kato, Mark T Gladwin, Oswaldo L Castro, Victor R Gordeuk
OBJECTIVES: We aimed to identify risk factors for acute pulmonary events in children and adolescents in the Pulmonary Hypertension and the Hypoxic Response in SCD (PUSH) study. METHODS: Patients with hemoglobin SS (n = 376) and other sickle cell genotypes (n = 127) aged 3-20 yrs were studied at four centers in a cross-sectional manner. A subgroup (n = 293) was followed for a median of 21 months (range 9-35). RESULTS: A patient-reported history of one or more acute pulmonary events, either acute chest syndrome (ACS) or pneumonia, was obtained in 195 hemoglobin SS patients (52%) and 51 patients with other genotypes (40%)...
July 2013: European Journal of Haematology
Bhavpreet S Dham, Daniel M Kwa, Joseph V Campellone
BACKGROUND CONTEXT: One hallmark of neurofibromatosis Type 1 (NF1) is the presence of multiple neurofibromas, which are hormonally responsive and may undergo rapid growth during periods of hormonal surge. Although spinal neurofibromas occur in up to 40% of patients with NF1, they rarely cause neurologic sequelae, especially in the young. PURPOSE: To document a unique case of acute postpartum paraparesis in a young woman because of an extradural neurofibroma compressing the conus medullaris and discuss the possible hormonal influences related to rapid growth of this otherwise "benign" nerve sheath tumor...
July 2012: Spine Journal: Official Journal of the North American Spine Society
J Forbes, D E Fetterolf
A retrospective case series demonstrating the use of dehydrated human amniotic membrane (dHAM) allografts in the treatment of wounds of various aetiologies. Amniotic membrane was applied to a series of chronic wounds referred to a formal wound clinic for aggressive management, after prior, traditional treatment methods were found ineffective, over a period of 1 month. In each case, failure of traditional therapy was followed by placement of a dehydrated amniotic membrane allograft and the healing time course was documented with charted measurements...
June 2012: Journal of Wound Care
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