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https://www.readbyqxmd.com/read/28859497/first-report-of-coexistence-of-ampc-beta-lactamase-genes-in-klebsiella-pneumoniae-strains-isolated-from-burn-patients
#1
Roya Ghanavati, Davood Darban-Sarokhalil, Fateme Navab-Moghadam, Hossein Kazemian, Gholamreza Irajian, Shabnam Razavi
Klebsiella spp. are among the most frequently isolated bacteria from burn wounds. These organisms are among the most important opportunistic pathogens, causing hospital-acquired and healthcare-associated infections worldwide. Limited information is available about prevalence of AmpC-producing Klebsiella pneumoniae from burn patients. Therefore, the aim of this study was to determine the characterization of AmpC beta-lactamase among K. pneumoniae isolated from burn patients. Samples were collected from wound specimens of patients with burn injury from a burn hospital in Tehran during 18 months (March 2015 to August 2016)...
September 1, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28735079/synthesis-and-characterization-of-nanoparticles-conjugated-tannase-and-using-it-for-enhancement-of-antibacterial-activity-of-tannase-produced-by-serratia-marcescens
#2
D Sahira Nsayef Muslim, Ziyad Abbas Dham, D Nadheer J Mohammed
Fourteen isolates of Serratia marcescens were collected from patients suffering from septicemia. All theseisolates revealed different levels in tannase production. Tannase was partially purified from Serratia marcescens b9 by precipitation method at 70% saturation of ammonium sulfate. Au, Pt, SnO2 and SiO2 nanoparticles were prepared by laser ablation and examined by transmission electron microscopy (TEM), X-ray diffraction pattern and UV-Visible absorption spectroscopy. Conjugation of SiO2 nanoparticles to tannase by feeding and pulses methods were prepared and characterized by TEM, X-ray diffraction pattern and UV-Visible spectrum...
July 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28718325/collaborative-care-for-psychiatric-disorders-in-older-adults-a-systematic-review
#3
Pallavi Dham, Sarah Colman, Karen Saperson, Carrie McAiney, Lillian Lourenco, Nick Kates, Tarek K Rajji
OBJECTIVE: To evaluate the mode of implementation, clinical outcomes, cost-effectiveness, and the factors influencing uptake and sustainability of collaborative care for psychiatric disorders in older adults. DESIGN: Systematic review. SETTING: Primary care, home health care, seniors' residence, medical inpatient and outpatient. PARTICIPANTS: Studies with a mean sample age of 60 years and older. INTERVENTION: Collaborative care for psychiatric disorders...
January 1, 2017: Canadian Journal of Psychiatry. Revue Canadienne de Psychiatrie
https://www.readbyqxmd.com/read/28532087/the-repairing-of-full-thickness-skin-deficiency-and-its-biological-mechanism-using-decellularized-human-amniotic-membrane-as-the-wound-dressing
#4
Mengsheng Song, Weiqing Wang, Qihua Ye, Shizhong Bu, Zhisen Shen, Yabin Zhu
Human amniotic membrane (HAM) was a biocompatible scaffold with advantages of anti-inflammatory, low antigen, feasibility, tolerance and low cost. In our previous work, HAM was treated to be decellularized using surfactant, lipase and DNAase methods and the efficacy as an implantable biological mesh was verified after decellularization treatment. In this work, we used the previous protocol to decellularize the fresh HAM, and applied it to repair full-thickness skin defects with Sprague-Dawley rats as the test animals...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28135201/use-of-a-dehydrated-amniotic-membrane-allograft-in-the-treatment-of-lower-extremity-wounds-a-retrospective-cohort-study
#5
Eric J Lullove
Injuries to the skin are extensively costly to the health care system. When caused by metabolic and vascular compromise, these injuries are even more foreboding for patients. They can result in chronic inflammation, reduced mobility, and chronic pain. MATERIALS AND METHODS: Twenty patients were selected from the author's patient population at the West Boca Center for Wound Healing for a retrospective cohort study. Patients underwent a run-in period of 2 weeks, where standard of care was used to clear the wound of bioburden...
January 23, 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/27995582/denudation-of-human-amniotic-membrane-by-a-novel-process-and-its-characterisations-for-biomedical-applications
#6
R Sripriya, R Kumar
This study was aimed to investigate the suitability of a modified method to get decellularised human amniotic membrane (DHAM). The obtained membrane was subjected to physico-chemical and biological evaluations to validate its potential for biomedical applications. The human amniotic membrane was processed with detergent and alkali followed by enzymatic treatments. Hematoxylin and eosin (H&E) and Masson's trichrome staining of membrane were in accordance with conjectures: the decellularised membrane stained for extracellular matrix is rich in collagen...
December 2016: Progress in biomaterials
https://www.readbyqxmd.com/read/26991266/myocardial-strain-can-be-measured-from-first-trimester-fetal-echocardiography-using-velocity-vector-imaging
#7
Anjali Chelliah, Niti Dham, Lowell H Frank, Mary Donofrio, Anita Krishnan
OBJECTIVES: Alterations in myocardial strain can identify cardiac dysfunction and can be measured in the mid-gestation fetus. This study evaluates feasibility of strain measurements in early fetuses and establishes normal early fetal strain values. METHODS: Normal fetal echocardiograms were reviewed for image adequacy for strain measurements in 12- to 14-week gestation fetuses. Two readers performed independent strain measurements. Values were compared with data from 40 normal second trimester fetuses...
May 2016: Prenatal Diagnosis
https://www.readbyqxmd.com/read/26918937/understanding-the-mechanism-of-the-hydrogen-abstraction-from-arachidonic-acid-catalyzed-by-the-human-enzyme-15-lipoxygenase-2-a-quantum-mechanics-molecular-mechanics-free-energy-simulation
#8
Reynier Suardíaz, Pablo G Jambrina, Laura Masgrau, Àngels González-Lafont, Edina Rosta, José M Lluch
Lipoxygenases (LOXs) are a family of enzymes involved in the biosynthesis of several lipid mediators. In the case of human 15-LOX, the 15-LOX-1 and 15-LOX-2 isoforms show slightly different reaction regiospecificity and substrate specificity, indicating that substrate binding and recognition may be different, a fact that could be related to their different biological role. Here, we have used long molecular dynamics simulations, QM(DFT)/MM potential energy and free energy calculations (using the newly developed DHAM method), to investigate the binding mode of the arachidonic acid (AA) substrate into 15-LOX-2 and the rate-limiting hydrogen-abstraction reaction 15-LOX-2 catalyzes...
April 12, 2016: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/26840647/decellularized-human-amniotic-membrane-how-viable-is-it-as-a-delivery-system-for-human-adipose-tissue-derived-stromal-cells
#9
M Gholipourmalekabadi, M Sameni, Dina Radenkovic, M Mozafari, M Mossahebi-Mohammadi, A Seifalian
OBJECTIVES: Human amniotic membrane (HAM) has been widely used in soft tissue engineering both in its fresh form and decellularized; its efficiency to aid treatment of burn injuries is well known. On the other hand, it has been reported clinically by several studies that human adipose-derived stem cells (hADSC) are a promising cell source for cell therapy for burns. Recently, we have reported a new technique for decellularization of HAM. In this study, potential of prepared decellularized HAM (dHAM) as a viable support for proliferation and delivery of hADSC was investigated...
February 2016: Cell Proliferation
https://www.readbyqxmd.com/read/26627477/deletion-of-the-2-acyl-glycerophosphoethanolamine-cycle-improve-glucose-metabolism-in-escherichia-coli-strains-employed-for-overproduction-of-aromatic-compounds
#10
César Aguilar, Noemí Flores, Fernando Riveros-McKay, Diana Sahonero-Canavesi, Susy Beatriz Carmona, Otto Geiger, Adelfo Escalante, Francisco Bolívar
BACKGROUND: As a metabolic engineering tool, an adaptive laboratory evolution (ALE) experiment was performed to increase the specific growth rate (µ) in an Escherichia coli strain lacking PTS, originally engineered to increase the availability of intracellular phosphoenolpyruvate and redirect to the aromatic biosynthesis pathway. As result, several evolved strains increased their growth fitness on glucose as the only carbon source. Two of these clones isolated at 120 and 200 h during the experiment, increased their μ by 338 and 373 %, respectively, compared to the predecessor PB11 strain...
2015: Microbial Cell Factories
https://www.readbyqxmd.com/read/26574225/free-energies-from-dynamic-weighted-histogram-analysis-using-unbiased-markov-state-model
#11
Edina Rosta, Gerhard Hummer
The weighted histogram analysis method (WHAM) is widely used to obtain accurate free energies from biased molecular simulations. However, WHAM free energies can exhibit significant errors if some of the biasing windows are not fully equilibrated. To account for the lack of full equilibration, we develop the dynamic histogram analysis method (DHAM). DHAM uses a global Markov state model to obtain the free energy along the reaction coordinate. A maximum likelihood estimate of the Markov transition matrix is constructed by joint unbiasing of the transition counts from multiple umbrella-sampling simulations along discretized reaction coordinates...
January 13, 2015: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/26351301/exposure-based-therapy-for-problem-gambling-in-a-patient-with-alzheimer-s-dementia
#12
Pallavi Dham, Amii Larsen, Michael Baigent
OBJECTIVES: This case report discusses the response to exposure and response prevention therapy in a patient with problem gambling and comorbid early dementia. It further discusses neurobiology of exposure therapy and its application in patients with mild cognitive impairment. CONCLUSION: Studies show good response to the use of exposure therapy in patients with cognitive impairment and further studies are needed to study the neurobiological changes and response to the therapy in patients with cognitive impairment...
October 2015: Australasian Psychiatry: Bulletin of Royal Australian and New Zealand College of Psychiatrists
https://www.readbyqxmd.com/read/26154915/incidence-and-mortality-trends-of-aspiration-pneumonia-in-parkinson-s-disease-in-the-united-states-1979-2010
#13
Umer Akbar, Bhavpreet Dham, Ying He, Nawaz Hack, Samuel Wu, Michelle Troche, Patrick Tighe, Eugene Nelson, Joseph H Friedman, Michael S Okun
INTRODUCTION: Careful examination of long-term analyses and trends is essential in understanding the medico-economic burden of this common complication. We sought to describe the long-term (32-year) trends of incidence and mortality in PD patients hospitalized with aspiration pneumonia (AsPNA). METHODS: Incidence and mortality of AsPNA in hospitalized PD versus non-PD patients was assessed by logistic regression analysis applied to a national database between the years 1979 and 2010...
September 2015: Parkinsonism & related Disorders
https://www.readbyqxmd.com/read/26046274/biocompatible-surgical-meshes-based-on-decellularized-human-amniotic-membrane
#14
Peina Shi, Mengna Gao, Qiuxia Shen, Lei Hou, Yabin Zhu, Jun Wang
Meshes play important roles to repair human tissue defect. In this work, human amniotic membrane (HAM) was decellularized and explored the efficacy as an implantable biological mesh. Surfactant, hypertonic saline, lipase and DNAase were used individually or collectively to remove all cell components and remain the extracellular matrix. Results of H&E and DAPI staining demonstrated that the method of surfactant and lipase combining with DNAase is the most effective treatment for HAM decellularization. Primary smooth muscle cells were seeded to evaluate the decellularized HAM's (dHAM) in vitro cytocompatibility...
September 2015: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/25884372/pediatric-dilated-cardiomyopathy-patients-do-not-meet-traditional-cardiac-resynchronization-criteria
#15
Ofer Schiller, Niti Dham, E Anne Greene, Deneen M Heath, Mark E Alexander, Charles I Berul
INTRODUCTION: Cardiac resynchronization therapy (CRT) is an effective device-based intervention for adults with heart failure (HF) with specific indications, based on large, multicenter randomized clinical trials. The criteria for CRT in adult HF include significant symptoms, ventricular systolic dysfunction, prolonged QRS duration, and left bundle branch block (LBBB) pattern on electrocardiogram (ECG). Despite having less data, CRT is also being widely utilized in children with HF. The shortage of evidence-based CRT criteria in pediatrics prompted us to review a cohort of children with dilated cardiomyopathy and evaluate their potential eligibility for CRT using the traditional adult criteria...
August 2015: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/25613153/dehydrated-human-amnion-chorion-membrane-allograft-nerve-wrap-around-the-prostatic-neurovascular-bundle-accelerates-early-return-to-continence-and-potency-following-robot-assisted-radical-prostatectomy-propensity-score-matched-analysis
#16
Vipul R Patel, Srinivas Samavedi, Anthony S Bates, Anup Kumar, Rafael Coelho, Bernardo Rocco, Kenneth Palmer
We present a propensity-matched analysis of patients undergoing placement of dehydrated human amnion/chorion membrane (dHACM) around the neurovascular bundle (NVB) during nerve-sparing (NS) robot-assisted laparoscopic prostatectomy (RARP). From March 2013 to July 2014, 58 patients who were preoperatively potent (Sexual Health Inventory for Men [SHIM] score >19) and continent (no pads) underwent full NS RARP. Postoperative outcomes were analyzed between propensity-matched graft and no-graft groups, including time to return to continence, potency, and biochemical recurrence...
June 2015: European Urology
https://www.readbyqxmd.com/read/25554534/analysis-of-clinical-parameters-and-cardiac-magnetic-resonance-imaging-as-predictors-of-outcome-in-pediatric-myocarditis
#17
Shagun Sachdeva, Xiaoyan Song, Niti Dham, Deneen M Heath, Roberta L DeBiasi
Myocarditis causes significant morbidity and mortality in pediatric patients, with potential adverse outcomes including heart failure, transplantation requirement, and/or death. The objective of this study was to determine predictors of early and late poor outcomes, defined as requirement for extracorporeal membrane oxygenation, ventricular assist device, transplantation, or death in pediatric myocarditis patients. A retrospective cohort study was conducted to evaluate pediatric myocarditis presenting over a 5-year period at a pediatric institution...
February 15, 2015: American Journal of Cardiology
https://www.readbyqxmd.com/read/25292796/carbon-dioxide-absorption-during-laparoscopic-inguinal-hernia-repair-tep-versus-tapp
#18
Ashok Gunawardene, Mohan Singh, Afzal Mohammed, Edward Harper, Dham Mobarak
No abstract text is available yet for this article.
October 2013: International Journal of Surgery
https://www.readbyqxmd.com/read/24519080/the-epidemiology-of-status-epilepticus-in-the-united-states
#19
Bhavpreet S Dham, Krystal Hunter, Fred Rincon
OBJECTIVE: To summarize trends in status epilepticus (SE) in the United States by age, race, sex, admission source, disposition, incidence rates, and mortality. METHODS: Data from US National Hospital Discharge Survey were used from 1979 to 2010 to identify discharges with SE and common etiologies and complications of SE using International Classification of Diseases, 9th Revision, Clinical Modifications codes. Temporal trends in the incidence and in-hospital mortality of SE were examined with respect to age, sex, and race...
June 2014: Neurocritical Care
https://www.readbyqxmd.com/read/24309610/abnormal-pulmonary-function-and-associated-risk-factors-in-children-and-adolescents-with-sickle-cell-anemia
#20
MULTICENTER STUDY
Manuel Arteta, Andrew Campbell, Mehdi Nouraie, Sohail Rana, Onyinye C Onyekwere, Gregory Ensing, Craig Sable, Niti Dham, Deepika Darbari, Lori Luchtman-Jones, Gregory J Kato, Mark T Gladwin, Oswaldo L Castro, Caterina P Minniti, Victor R Gordeuk
Obstructive and restrictive pulmonary changes develop in children with sickle cell disease, but reports conflict as to the type of change that predominates. We prospectively performed spirometry, plethysmography, and lung diffusing capacity in 146 children aged 7 to 20 years with hemoglobin SS or Sβ(0)-thalassemia. Nineteen percent of the patients had obstructive physiology as defined according to guidelines of the American Thoracic Society. In addition, 9% had restrictive physiology and 11% had abnormal but not categorized physiology...
April 2014: Journal of Pediatric Hematology/oncology
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