keyword
MENU ▼
Read by QxMD icon Read
search

PRG4

keyword
https://www.readbyqxmd.com/read/29772480/proteoglycan-4-regulates-macrophage-function-without-altering-atherosclerotic-lesion-formation-in-a-murine-bone-marrow-specific-deletion-model
#1
Joya E Nahon, Menno Hoekstra, Stefan R Havik, Peter J Van Santbrink, Geesje M Dallinga-Thie, Jan-Albert Kuivenhoven, Janine J Geerling, Miranda Van Eck
BACKGROUND AND AIMS: Proteoglycan 4 (Prg4) has a high structural similarity with the established atherosclerosis-modulating proteoglycan versican, but its role in atherogenesis is still unknown. Therefore, the impact of Prg4 deficiency on macrophage function in vitro and atherosclerosis susceptibility in vivo was investigated. METHODS: The presence and localization of Prg4 was studied in atherosclerotic lesions. Furthermore, the effect of Prg4 deficiency on macrophage foam cell formation, cholesterol efflux and lipopolysaccharide (LPS) response was determined...
May 5, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29575101/adherent-agarose-mold-cultures-an-in-vitro-platform-for-multi-factorial-assessment-of-passaged-chondrocyte-redifferentiation
#2
Justin Parreno, Vanessa J Bianchi, Corey Sermer, Suresh C Regmi, David Backstein, Tannin A Schmidt, Rita A, Kandel
Generating the best possible bioengineered cartilage from passaged chondrocytes requires culture condition optimization. In this study the use of adherent agarose mold (adAM) cultures to support redifferentiation of passaged twice (P2) chondrocytes and serve as a scalable platform to assess the effect of growth factor combinations on proteoglycan accumulation by cells was examined. By 2 days in adAM culture, bovine P2 cells were partially redifferentiated as demonstrated by regression of actin-based dedifferentiation signalling and fibroblast matrix and contractile gene expression...
March 25, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29537109/cdc42-regulates-the-expression-of-superficial-zone-molecules-in-part-through-the-actin-cytoskeleton-and-myocardin-related-transcription-factor-a
#3
Elizabeth Delve, Justin Parreno, Vivian Co, Po-Han Wu, Jasmine Chong, Matteo Di Scipio, Rita A Kandel
Osteoarthritis (OA) is a degenerative disease that initially manifests as loss of the superficial zone (SZ) of articular cartilage. SZ chondrocytes (SZC) differ in morphology from other chondrocytes as they are elongated and oriented parallel to the tissue surface. Proteoglycan 4 (PRG4) and tenascin C (TNC) are molecules expressed by SZC, which have been shown to be chondroprotective. Identification of the signalling pathway(s) regulating expression of SZ molecules may lead to a therapeutic target that can be used to delay or prevent the onset of OA...
March 14, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29512526/-homeostasis-and-disorder-of-musculoskeletal-system-mechanisms-of-cartilage-development-and-homeostasis
#4
Riko Nishimura, Kenji Hata, Eriko Nakamura
Chondrocytes, which are originated from undifferentiated mesenchymal stem cells, play roles in skeletal development and growth in mammal, and smooth movement of joints. Endochondral ossification is necessary for skeletal development, and the multiple and complex biological events are precisely regulated by several hormones, cytokines, and their downstream signaling and transcriptional regulation. On the other hands, articular chondrocytes physiologically retains their features during a lifetime. Numerous molecules involved in endochondral ossification have been identified and investigation of the molecular mechanisms have amazingly progressed...
2018: Clinical Calcium
https://www.readbyqxmd.com/read/29444976/foxo-transcription-factors-modulate-autophagy-and-proteoglycan-4-in-cartilage-homeostasis-and-osteoarthritis
#5
Tokio Matsuzaki, Oscar Alvarez-Garcia, Sho Mokuda, Keita Nagira, Merissa Olmer, Ramya Gamini, Kohei Miyata, Yukio Akasaki, Andrew I Su, Hiroshi Asahara, Martin K Lotz
Aging is a main risk factor for osteoarthritis (OA). FoxO transcription factors protect against cellular and organismal aging, and FoxO expression in cartilage is reduced with aging and in OA. To investigate the role of FoxO in cartilage, Col2Cre-FoxO1, 3, and 4 single knockout (KO) and triple KO mice (Col2Cre-TKO) were analyzed. Articular cartilage in Col2Cre-TKO and Col2Cre-FoxO1 KO mice was thicker than in control mice at 1 or 2 months of age. This was associated with increased proliferation of chondrocytes of Col2Cre-TKO mice in vivo and in vitro...
February 14, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29415892/inhibition-of-wnt-%C3%AE-catenin-signaling-ameliorates-osteoarthritis-in-a-murine-model-of-experimental-osteoarthritis
#6
Caressa Lietman, Brian Wu, Sarah Lechner, Andrew Shinar, Madhur Sehgal, Evgeny Rossomacha, Poulami Datta, Anirudh Sharma, Rajiv Gandhi, Mohit Kapoor, Pampee P Young
Osteoarthritis (OA) is a degenerative joint disease involving both cartilage and synovium. The canonical Wnt/β-catenin pathway, which is activated in OA, is emerging as an important regulator of tissue repair and fibrosis. This study seeks to examine Wnt pathway effects on synovial fibroblasts and articular chondrocytes as well as the therapeutic effects of Wnt inhibition on OA disease severity. Mice underwent destabilization of the medial meniscus surgery and were treated by intra-articular injection with XAV-939, a small-molecule inhibitor of Wnt/β-catenin signaling...
February 8, 2018: JCI Insight
https://www.readbyqxmd.com/read/29397575/genotype-phenotype-investigation-of-35-patients-from-11-unrelated-families-with-camptodactyly-arthropathy-coxa-vara-pericarditis-cacp-syndrome
#7
Saliha Yilmaz, Dilek Uludağ Alkaya, Özgür Kasapçopur, Kenan Barut, Ekin S Akdemir, Cemre Celen, Mark W Youngblood, Katsuhito Yasuno, Kaya Bilguvar, Murat Günel, Beyhan Tüysüz
BACKGROUND: The camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP) is a rare autosomal recessive condition characterized by camptodactyly, noninflammatory arthropathy, coxa vara, and pericarditis. CACP is caused by mutations in the proteoglycan 4 (PRG4) gene, which encodes a lubricating glycoprotein present in the synovial fluid and at the surface of articular cartilage. METHODS: In the present study, we compared the clinical and molecular findings of CACP syndrome in 35 patients from 11 unrelated families...
March 2018: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/29381614/bone-conditioned-medium-obtained-from-calvaria-mandible-and-tibia-cause-an-equivalent-tgf-%C3%AE-1-response-in-vitro
#8
Babak Hatami Kia, João Rui Galvão Mendes, Heinz-Dieter Müller, Patrick Heimel, Reinhard Gruber
Bones with different embryological origin and mode of ossification are supposed to vary in their capacity for supporting graft consolidation. The aim of the current pilot study was to assess the TGF-β1 activity of bone chips obtained from distinct anatomic locations. Conditioned medium was prepared from bone chips harvested from pig calvaria, mandible, and tibia. Human oral fibroblasts were exposed to bone-conditioned medium (BCM) followed by reverse transcriptase polymerase chain reaction of the TGF-β1 target genes...
January 19, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29080679/sfrp2-dpp4-and-fmo1-lsp1-define-major-fibroblast-populations-in-human-skin
#9
Tracy Tabib, Christina Morse, Ting Wang, Wei Chen, Robert Lafyatis
Fibroblasts produce matrix, regulate inflammation, mediate reparative processes, and serve as pluripotent mesenchymal cells. Analyzing digested normal human skin by single-cell RNA sequencing, we explored different fibroblast populations. T-distributed stochastic neighbor embedding and clustering of single-cell RNA sequencing data from six biopsy samples showed two major fibroblast populations, defined by distinct genes, including SFRP2 and FMO1, expressed exclusively by these two major fibroblast populations...
April 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28975281/genotype-phenotype-associations-of-il6-and-prg4-with-conjunctival-fibrosis-after-glaucoma-surgery
#10
Cynthia Yu-Wai-Man, Aristides D Tagalakis, Jinhong Meng, Yann Bouremel, Richard M H Lee, Alex Virasami, Stephen L Hart, Peng T Khaw
Importance: Postsurgical fibrosis is a critical determinant of the long-term success of glaucoma surgery, but no reliable biomarkers are currently available to stratify the risk of scarring. Objective: To compare the clinical phenotype of patients with conjunctival fibrosis after glaucoma surgery with candidate gene expression tissue biomarkers of fibrosis. Design, Setting, and Participants: In this cross-sectional study, 42 patients were recruited at the time of glaucoma surgery at the Moorfields Eye Hospital from September 1, 2014, to September 1, 2016...
November 1, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28914993/hyaluronan-incorporation-into-model-contact-lens-hydrogels-as-a-built-in-lubricant-effect-of-hydrogel-composition-and-proteoglycan-4-as-a-lubricant-in-solution
#11
Michael Samsom, Myrto Korogiannaki, Lakshman N Subbaraman, Heather Sheardown, Tannin A Schmidt
Contact lens friction significantly correlates with subjective comfort. Hyaluronan (HA) and proteoglycan 4 (PRG4) are natural boundary lubricants present in the body. The objective of this study was to assess the effect of crosslinked HA into the bulk of model contact lens materials pHEMA, pHEMA/TRIS, and DMAA/TRIS on surface wettability, protein sorption, and boundary lubricating properties at a material-cornea biointerface, both alone and synergistically with PRG4 in solution. Surface wettability was assessed by water contact angle measurement, protein sorption by lysozyme sorption assay, and boundary lubricating properties using an in vitro friction test method...
September 15, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28852125/irf2bp2-deficient-microglia-block-the-anxiolytic-effect-of-enhanced-postnatal-care
#12
Aswin Hari, Shelly A Cruz, Zhaohong Qin, Pascal Couture, Ragnar O Vilmundarson, Hua Huang, Alexandre F R Stewart, Hsiao-Huei Chen
Enhanced postnatal care (EPC) increases resilience to adversity in adulthood. Since microglia participate in shaping neural circuits, we asked how ablation of an inflammation-suppressing factor IRF2BP2 (Interferon Regulatory Factor 2 Binding Protein 2) in microglia would affect the responses to EPC. Mice lacking IRF2BP2 in microglia (KO) and littermate controls (WT) were subjected to EPC during the first 3 weeks after birth. EPC reduced anxiety in WT but not KO mice. This was associated with reduced inflammatory cytokine expression in the hypothalamus...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28782725/the-bio-in-the-ink-cartilage-regeneration-with-bioprintable-hydrogels-and-articular-cartilage-derived-progenitor-cells
#13
Riccardo Levato, William R Webb, Iris A Otto, Anneloes Mensinga, Yadan Zhang, Mattie van Rijen, René van Weeren, Ilyas M Khan, Jos Malda
Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration...
August 4, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28716756/cartilage-specific-deletion-of-alk5-gene-results-in-a-progressive-osteoarthritis-like-phenotype-in-mice
#14
Q Wang, Q Y Tan, W Xu, H B Qi, D Chen, S Zhou, Z H Ni, L Kuang, J Y Guo, J L Huang, X X Wang, Z Q Wang, N Su, L Chen, B Chen, W L Jiang, Y Gao, H G Chen, X L Du, Y L Xie, L Chen
OBJECTIVE: Previous studies have shown that Transforming growth factor-β (TGF-β)/TGFβRII-Smad3 signaling is involved in articular cartilage homeostasis. However, the role of TGF-β/ALK5 signaling in articular cartilage homeostasis has not been fully defined. In this study, a combination of in vitro and in vivo approaches was used to elucidate the role of ALK5 signaling in articular cartilage homeostasis and the development of osteoarthritis (OA). DESIGN: Mice with inducible cartilage-specific deletion of Alk5 were generated to assess the role of ALK5 in OA development...
November 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28688149/proteoglycan-4-and-hyaluronan-as-boundary-lubricants-for-model-contact-lens-hydrogels
#15
Michael Samsom, Yuno Iwabuchi, Heather Sheardown, Tannin A Schmidt
Clinical data show that in vitro contact lens friction is related to in vivo comfort. Solutions of biological lubricants hyaluronan (HA) and proteoglycan 4 (PRG4, also known as lubricin) reduce friction at a cornea-polydimethylsiloxane (PDMS) interface. The purpose of this study was to (1) determine if PRG4 can sorb to and lubricate model contact lens materials and (2) assess the boundary lubricating ability of PRG4 and HA compared to saline on model contact lens materials. PRG4 was obtained from bovine cartilage culture and suspended in saline at 300 µg/mL...
April 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28604608/friction-induced-mitochondrial-dysregulation-contributes-to-joint-deterioration-in-prg4-knockout-mice
#16
Kimberly A Waller, Ling X Zhang, Gregory D Jay
Deficiency of PRG4 (lubricin), the boundary lubricant in mammalian joints, contributes to increased joint friction accompanied by superficial and upper intermediate zone chondrocyte caspase-3 activation, as shown in lubricin-null (Prg4(-/-)) mice. Caspase-3 activity appears to be reversible upon the restitution of Prg4 either endogenously in vivo, in a gene trap mouse, or as an applied lubricant in vitro. In this study we show that intra-articular injection of human PRG4 in vivo in Prg4(-/-) mice prevented caspase-3 activation in superficial zone chondrocytes and was associated with a modest decrease in whole joint friction measured ex vivo using a joint pendulum method...
June 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28579418/early-genetic-restoration-of-lubricin-expression-in-transgenic-mice-mitigates-chondrocyte-peroxynitrite-release-and-caspase-3-activation
#17
K M Larson, L Zhang, G J Badger, G D Jay
OBJECTIVE: This study investigated the ability of endogenous lubricin secretion to restore joint health following a brief <21 day, postnatal lubricin-null state, in a C57BL/6J Prg4 gene trap (GT) mouse under the control of cre-recombinase. Previously we showed that re-expression of lubricin at 21 days was partly restorative of joint lubrication. DESIGN: The tibio-femoral joints of adult C57BL/6J mice containing lubricin, lacking lubricin, and postnatally lacking lubricin until restoration of lubricin expression at 7 days or 14 days of age were evaluated ex vivo...
September 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28578597/optimization-of-methods-for-articular-cartilage-surface-tissue-engineering-cell-density-and-transforming-growth-factor-beta-are-critical-for-self-assembly-and-lubricin-secretion
#18
Kenjiro Iwasa, A Hari Reddi
OBJECTIVE: Lubricin/superficial zone protein (SZP)/proteoglycan4 (PRG4) plays an important role in boundary lubrication in articular cartilage. Lubricin is secreted by superficial zone chondrocytes and synoviocytes of the synovium. The specific objective of this investigation is to optimize the methods for tissue engineering of articular cartilage surface. The aim of this study is to investigate the effect of cell density on the self-assembly of superficial zone chondrocytes and lubricin secretion as a functional assessment...
July 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28549901/degradation-of-proteoglycan-4-lubricin-by-cathepsin-s-potential-mechanism-for-diminished-ocular-surface-lubrication-in-sj%C3%A3-gren-s-syndrome
#19
Suresh C Regmi, Michael L Samsom, Miriam L Heynen, Gregory D Jay, Benjamin D Sullivan, Sruthi Srinivasan, Barbara Caffery, Lyndon Jones, Tannin A Schmidt
Sjögren's syndrome (SS) is an autoimmune disease affecting the lacrimal and salivary glands with hallmark clinical symptoms of dry eye and dry mouth. Recently, markedly increased cathepsin S (CTSS) activity has been observed in the tears of SS patients. Proteoglycan 4 (PRG4), also known as lubricin, is an effective boundary lubricant that is naturally present on the ocular surface. While PRG4 is susceptible to proteolytic digestion, the potential effect of CTSS on PRG4 remains unknown. The objective of this study was to assess the ability of CTSS to enzymatically degrade purified PRG4, and PRG4 naturally present in human tears, and alter ocular surface boundary lubricating properties...
August 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28482921/the-autocrine-role-of-proteoglycan-4-prg4-in-modulating-osteoarthritic-synoviocyte-proliferation-and-expression-of-matrix-degrading-enzymes
#20
Ali Alquraini, Maha Jamal, Ling Zhang, Tannin Schmidt, Gregory D Jay, Khaled A Elsaid
BACKGROUND: Lubricin/proteoglycan 4 (PRG4) is a mucinous glycoprotein secreted by synovial fibroblasts and superficial zone chondrocytes. Recently, we showed that recombinant human PRG4 (rhPRG4) is a putative ligand for CD44 receptor. rhPRG4-CD44 interaction inhibits cytokine-induced rheumatoid arthritis synoviocyte proliferation. The objective of this study is to decipher the autocrine function of PRG4 in regulating osteoarthritic synoviocyte proliferation and expression of catabolic and pro-inflammatory mediators under basal and interleukin-1 beta (IL-1β)-stimulated conditions...
May 8, 2017: Arthritis Research & Therapy
keyword
keyword
22535
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"