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Enterobacter cloacae

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https://www.readbyqxmd.com/read/29351668/evaluation-of-the-rapid-carbapenem-inactivation-method-rcim-a-phenotypic-screening-test-for-carbapenemase-producing-enterobacteriaceae
#1
Madalina-Maria Muntean, Andrei-Alexandru Muntean, Lauraine Gauthier, Elodie Creton, Garance Cotellon, Mircea Ioan Popa, Rémy A Bonnin, Thierry Naas
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection. Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain...
January 16, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29349568/class-iii-bacteriocin-helveticin-m-causes-sublethal-damage-on-target-cells-through-impairment-of-cell-wall-and-membrane
#2
Zhilan Sun, Xiaomeng Wang, Xinxiao Zhang, Haihong Wu, Ye Zou, Pengpeng Li, Chong Sun, Weimin Xu, Fang Liu, Daoying Wang
Helveticin-M, a novel Class III bacteriocin produced by Lactobacillus crispatus exhibited an antimicrobial activity against Staphylococcus aureus, S. saprophyticus, and Enterobacter cloacae. To understand how Helveticin-M injured target cells, Helveticin-M was cloned and heterologously expressed in Escherichia coli. Subsequently, the cell wall organization and cell membrane integrity of target cells were determined. The mechanism of cellular damage differed according to bacterial species. Based on morphology analysis, Helveticin-M disrupted the cell wall of Gram-positive bacteria and disorganized the outer membrane of Gram-negative bacteria, therefore, altering surface structure...
January 18, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29344811/metabolic-evolution-and-a-comparative-omics-analysis-of-corynebacterium-glutamicum-for-putrescine-production
#3
Zhen Li, Yu-Ping Shen, Xuan-Long Jiang, Li-Shen Feng, Jian-Zhong Liu
Putrescine is widely used in the industrial production of bioplastics, pharmaceuticals, agrochemicals, and surfactants. Because the highest titer of putrescine is much lower than that of its precursor L-ornithine reported in microorganisms to date, further work is needed to increase putrescine production in Corynebacterium glutamicum. We first compared 7 ornithine decarboxylase genes and found that the Enterobacter cloacae ornithine decarboxylase gene speC1 was most suitable for putrescine production in C. glutamicum...
January 17, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29343795/recurrent-wheezing-in-neonatal-pneumonia-is-associated-with-combined-infection-with-respiratory-syncytial-virus-and-staphylococcus-aureus-or-klebsiella-pneumoniae
#4
Qin Zhong, Hui Feng, Qi Lu, Xu Liu, Qi Zhao, Yue Du, Xian-Hong Zhang, Jia-Rong Wang
Both viral and bacterial infections can be associated with wheezing episodes in children; however, information regarding combined infections with both viral and bacterial pathogens in full term neonates is limited. We sought to investigate the effects of viral-bacterial codetection on pneumonia severity and recurrent wheezing. A retrospective cohort study was conducted on neonates admitted to our hospital with pneumonia from 2009 to 2015. Of 606 total cases, 341 were diagnosed with RSV only, and 265 were diagnosed with both RSV and a potential bacterial pathogen...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29342994/-report-of-antimicrobial-resistance-surveillance-program-in-chinese-children-in-2016
#5
C Q Wang, A M Wang, H Yu, H M Xu, C M Jing, J K Deng, R Z Zhao, C Z Hua, Y H Chen, X J Chen, T Zhang, H Zhang, Y P Chen, J G Yang, A W Lin, S F Wang, Q Cao, X Wang, H L Deng, S C Cao, J H He, W Gao, S Z Han
Objective: To analyze the antimicrobial resistance profile in Chinese children. Methods: This was a prevalence survey. From January 1 through December 31, 2016, the isolates were collected from 10 tertiary children hospitals in China. Antimicrobial susceptibility testing was carried out by routine laboratory methods. The penicillin susceptibility of streptococcus pneumonia and Meropenem susceptibility of gram-negative bacteria were detected by E-test and disk diffusion method respectively. Antimicrobial susceptibility results were interpreted according to the criteria of Clinical and Laboratory Standards Institute (CLSI) Guideline 2016...
January 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/29320234/influence-of-soap-characteristics-and-food-service-facility-type-on-the-degree-of-bacterial-contamination-of-open-refillable-bulk-soaps
#6
Donald W Schaffner, Dane Jensen, Charles P Gerba, David Shumaker, James W Arbogast
Concern has been raised regarding the public health risks from refillable bulk-soap dispensers because they provide an environment for potentially pathogenic bacteria to grow. This study surveyed the microbial quality of open refillable bulk soap in four different food establishment types in three states. Two hundred ninety-six samples of bulk soap were collected from food service establishments in Arizona, New Jersey, and Ohio. Samples were tested for total heterotrophic viable bacteria, Pseudomonas, coliforms and Escherichia coli, and Salmonella...
January 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29311089/molecular-characterization-of-imp-1-producing-enterobacter-cloacae-complex-isolates-in-tokyo
#7
Kotaro Aoki, Sohei Harada, Koji Yahara, Yoshikazu Ishii, Daisuke Motooka, Shota Nakamura, Yukihiro Akeda, Tetsuya Iida, Kazunori Tomono, Satoshi Iwata, Kyoji Moriya, Kazuhiro Tateda
Although KPC enzymes are most common among carbapenemases produced by Enterobacter cloacae complex globally, the epidemiology varies from one country to another. While previous studies have suggested that IMP enzymes are most common in Japan, detailed analysis has been scarce thus far. Here we carried out a molecular epidemiological study and plasmid analysis of IMP-1-producing E. cloacae complex isolates collected from 3 hospitals in central Tokyo using whole genome sequencing. Seventy-one isolates were classified into several STs and 49 isolates were identified as Enterobacter hormaechei ST78...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29311088/antibiotic-resistance-plasmids-cointegrated-into-a-megaplasmid-harbouring-the-blaoxa-427-carbapenemase-gene
#8
Stefanie Desmet, Suruchi Nepal, Jan Maarten van Dijl, Marc Van Ranst, Monika A Chlebowicz, John W Rossen, Jeroen K J Van Houdt, Piet Maes, Katrien Lagrou, Erik Bathoorn
OXA-427 is a new class-D carbapenemase encountered in different species of Enterobacteriaceae in a Belgian hospital. To study the dispersal of this gene, we describe the comparative analysis of two plasmids containing the blaOXA-427 gene isolated from a Klebsiella pneumoniae and an Enterobacter cloacae complex strain. The two IncA/C2 plasmids containing blaOXA-427 share the same backbone, however, in the K. pneumoniae this plasmid is cointegrated into an IncFIB plasmid forming a 321 kb megaplasmid with multiple multi-resistance regions...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29304717/analysis-of-the-spectrum-and-antibiotic-resistance-of-uropathogens-in-outpatients-at-a-tertiary-hospital
#9
Bo Yang, Fujia Yang, Shanmei Wang, Qian Wang, Zhirui Liu, Wei Feng, Fengjun Sun, Peiyuan Xia
The objective of this study was to analyse the distribution and antimicrobial resistance of bacterial uropathogens isolated from outpatients at Henan Provincial People's Hospital. A total of 1419 samples from 823 newly diagnosed and 596 recurrent UTI outpatients culture positive. Escherichia coli was the most common uropathogen. Compared with the recurrent group, the newly diagnosed group had a higher isolation rate of E. coli and Enterobacter cloacae but a lower isolation rate of Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter spp...
January 5, 2018: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29298099/propidium-monoazide-quantitative-real-time-polymerase-chain-reaction-for-enumeration-of-some-viable-but-nonculturable-foodborne-bacteria-in-meat-and-meat-products
#10
Norhan Khairy Abd El-Aziz, Yasmine Hasanine Tartor, Ahlam Abd El-Aziz Gharib, Ahmed Mohamed Ammar
Foodborne infections due to bacterial pathogens are increasing worldwide. Given the surreptitious nature of viable but nonculturable (VBNC) bacteria, they largely remain a threat to public health and food safety due to their non-detectability through conventional plate count techniques. Hence, species-specific quantitative real-time polymerase chain reaction (PCR) (qPCR) alone and combined with the use of propidium monoazide (PMA) was used along with the plate count method to quantify VBNC Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, and Enterobacteriaceae in fresh and processed meat samples...
January 3, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29286016/granulated-sugar-for-adjuvant-treatment-of-surgical-wound-infection-due-to-multi-drug-resistant-pathogens-in-a-child-with-sarcoma-a-case-report-and-literature-review
#11
Aldo Naselli, Laura Accame, Piero Buffa, Anna Loy, Roberto Bandettini, Alberto Garaventa, Ornella Della Casa Alberighi, Elio Castagnola
The use of sugar for treating wounds which are difficult to heal and positive to resistant pathogens has already been documented. The authors describe the successful treatment by direct instillation of granular sugar in the antibiotic-resistant infected surgical site wound of a child with sarcoma. Sugar instillation in the extended spectrum beta-lactamase (ESBL)-producing Enterobacter cloacae positive wound, in addition to systemic treatment with meropenem and levofloxacin, allowed culture negativization in six days and complete wound healing in 30 days...
December 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/29279516/spontaneous-development-of-acute-obstructive-suppurative-pancreatic-ductitis-associated-with-pancreatic-carcinoma-a-first-case-report
#12
Kunio Iwatsuka, Hiroshi Nakagawara, Masahiro Ogawa, Takuji Gotoda, Shigeoki Hayashi, Noriko Kinukawa, Akihiro Hemmi, Kenji Yamao, Akio Yanagisawa, Mitsuhiko Moriyama
A 68-year-old man with a history of diabetes mellitus was admitted to our hospital with a diagnosis of acute pancreatitis. Abdominal computed tomography revealed a suspicious tumor in the body of the pancreas, along with a dilated main pancreatic duct and edema of the pancreatic tail. Endoscopic retrograde pancreatography was performed after treating the patient's pancreatitis. When a cannula tip was advanced beyond the stenosis, deep into the distal pancreatic duct, thick white pus was evacuated. A bacteriological examination of the aspirated pancreatic juice revealed Enterobacter cloacae, and a cytological examination revealed adenocarcinoma...
December 27, 2017: Internal Medicine
https://www.readbyqxmd.com/read/29274468/molecular-characterization-of-carbapenem-resistant-enterobacter-cloacae-complex-in-colombia-blakpc-and-the-changing-landscape
#13
Elsa De La Cadena, Adriana Correa, Juan Sebastián Muñoz, Laura J Rojas, Cristhian Hernández-Gómez, Christian Pallares, Federico Perez, Robert A Bonomo, Maria V Villegas
OBJECTIVES: To examine the population structure of representative carbapenem resistant Enterobacter cloacae complex (CR-Ecl) isolates from 8 different Colombian regions and to characterize their asociated β-lactamases. METHODS: Twenty-eight CR-Ecl isolates collected in Colombia between 2009 and 2013 through the Colombian Nosocomial Network were included. Antimicrobial susceptibility testing was performed by broth microdilution. Molecular detection of carbapenemases and extended-spectrum β-lactamases (ESBL), and the presence of Tn4401 was evaluated by PCR and sequencing...
December 20, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29258559/immune-related-redox-metabolism-of-embryonic-cells-of-the-tick-rhipicephalus-microplus-bme26-in-response-to-infection-with-anaplasma-marginale
#14
Sandra Patricia Kalil, Rafael Diego da Rosa, Janaína Capelli-Peixoto, Paula Cristiane Pohl, Pedro Lagerblad de Oliveira, Andrea Cristina Fogaça, Sirlei Daffre
BACKGROUND: It is well known that reactive oxygen species (ROS) and reactive nitrogen species (RNS) are involved in the control of pathogens and microbiota in insects. However, the knowledge of the role of ROS and RNS in tick-pathogen and tick-microbiota interactions is limited. Here, we evaluated the immune-related redox metabolism of the embryonic cell line BME26 from the cattle tick Rhipicephalus microplus in response to Anaplasma marginale infection. METHODS: A high-throughput qPCR approach was used to determine the expression profile of 16 genes encoding proteins involved in either production or detoxification of ROS and RNS in response to different microbial challenges...
December 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29248504/rapid-emergence-of-colistin-resistance-and-its-impact-on-mortality-in-healthcare-associated-infections
#15
Mehtap Aydin, Önder Ergönül, Alpay Azap, Hüseyin Bilgin, Güle Aydın, Sema Alp Çavuş, Yusuf Ziya Demiroğlu, Hikmet Eda Çalışkan, Osman Memikoğlu, Şirin Menekşe, Şafak Kaya, Nazlım Aktuğ Demir, İlkay Karaoğlan, Seniha Başaran, Çiğdem Hatipoğlu, Şebnem Erdinç, Emel Yılmaz, Ayhanım Tümtürk, Yasemin Tezer, Hamiyet Demirkaya, Şule Eren Çakar, Şiran Keske, SudaTekin, Cem Yardımcı, Çağla Karakoç, Pınar Ergen, Özlem Azap, Lütfiye Mülazımoğlu, Onur Ural, Füsun Can, Halis Akalın
We describe the emergence of resistance and predictors of fatality for 1556 cases of healthcare-associated Gram-negative bloodstream infections in 2014 and 2015. The colistin resistance rate in Klebsiella pneumoniae was 16.1%, compared with 6% in 2013. 660 (42.4%) cases were fatal. The highest fatality rate was among patients with Acinetobacter baumannii bacteremia (58%), followed by Pseudomonas aeruginosa (45%), Klebsiella pneumoniae (41%), Enterobacter cloacae (32%), and Escherichia coli (28%). In multivariate analysis, MIC for carbapenem (OR:1...
December 14, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29244831/antimicrobial-susceptibility-and-molecular-epidemiology-of-clinical-enterobacter-cloacae-bloodstream-isolates-in-shanghai-china
#16
Su Wang, Shu-Zhen Xiao, Fei-Fei Gu, Jin Tang, Xiao-Kui Guo, Yu-Xing Ni, Jie-Ming Qu, Li-Zhong Han
BACKGROUND: Enterobacter cloacae is a major nosocomial pathogen causing bloodstream infections. We retrospectively conducted a study to assess antimicrobial susceptibility and phylogenetic relationships of E. cloacae bloodstream isolates in two tertiary university-affiliated hospitals in Shanghai, in order to facilitate managements of E. cloacae bloodstream infections and highlight some unknowns for future prevention. METHODS: Fifty-three non-duplicate E. cloacae bloodstream isolates were consecutively collected from 2013 to 2016...
2017: PloS One
https://www.readbyqxmd.com/read/29237086/enterobacteriaceae-producing-oxa-48-like-carbapenemases-in-poland-2013-january-2017
#17
R Izdebski, A Baraniak, D Zabicka, M Machulska, P Urbanowicz, J Fiett, E Literacka, K Bojarska, A Kozinska, B Zieniuk, W Hryniewicz, M Gniadkowski
Objectives: To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Methods: Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types and other β-lactamases were amplified and sequenced. Mobile elements with blaOXA-48/181-like genes were PCR mapped. blaOXA-48/181-carrying plasmids were characterized by nuclease S1-hybridization profiling, transfer assays and PCR-based replicon typing, while the chromosomal location of the genes was confirmed by the I-CeuI analysis...
December 9, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29237003/illumina-short-read-and-minion-long-read-wgs-to-characterize-the-molecular-epidemiology-of-an-ndm-1-serratia-marcescens-outbreak-in-romania
#18
H T T Phan, N Stoesser, I E Maciuca, F Toma, E Szekely, M Flonta, A T M Hubbard, L Pankhurst, T Do, T E A Peto, A S Walker, D W Crook, D Timofte
Background and Objectives: Serratia marcescens is an emerging nosocomial pathogen, and the carbapenemase blaNDM has been reported in several surveys in Romania. We aimed to investigate the molecular epidemiology of S. marcescens in two Romanian hospitals over 2010-15, including a neonatal NDM-1 S. marcescens outbreak. Methods: Isolates were sequenced using Illumina technology together with carbapenem-non-susceptible NDM-1-positive and NDM-1-negative Klebsiella pneumoniae and Enterobacter cloacae to provide genomic context...
December 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29214986/antibiotic-resistance-in-enterobacter-cloacae-strains-with-derepressed-partly-derepressed-inducible-ampc-and-extendedspectrum-beta-lactamases-in-zenica-doboj-canton-bosnia-and-herzegovina
#19
Selma Uzunović, Amir Ibrahimagić, Branka Bedenić
Aim To investigate the prevalence of derepressed/partly derepressed/inducible and ESBL/AmpC-producing Enterobacter cloacae isolates and treatment options for infections associated with those isolates. Methods Antibiotic susceptibility was determined by disc diffusion and broth microdilution according to CLSI guidelines. Doubledisk synergy test (DDST) was performed in order to screen for ESBLs and combined disk test with phenylboronic acid to detect AmpC β -lactamases. PCR was used to detect blaESBL/blacarb genes...
February 1, 2018: Medicinski Glasnik
https://www.readbyqxmd.com/read/29211819/genomic-investigation-of-a-putative-endoscope-associated-carbapenem-resistant-enterobacter-cloacae-outbreak-reveals-a-wide-diversity-of-circulating-strains-and-resistance-mutations
#20
Shawn E Hawken, Laraine L Washer, Christopher L Williams, Duane W Newton, Evan S Snitkin
A genomic epidemiologic investigation of a putative carbapenem-resistant Enterobacter cloacae outbreak revealed few plausible instances of nosocomial transmission, highlighting instead the frequent importation of E. cloacae into our hospital. Searching for genetic determinants of carbapenem resistance demonstrated that most resistance is due to convergent mutations in phylogenetically diverse E. cloacae.
October 26, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
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