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Enterobacter cloacae

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https://www.readbyqxmd.com/read/29753261/removal-enactment-of-organo-phosphorous-pesticide-using-bacteria-isolated-from-domestic-sewage
#1
Md Shabbir, Mukesh Singh, Swati Maiti, Sunil Kumar, Samar K Saha
Three bacteria (MS I, II and III) i.e., Pseudomonas aeruginosa (KY781886), Enterobactor ludwigii (KX881423) and Enterobacter cloacae (KX881513) isolated from domestic sewage were identified on the basis of 16S rDNA sequencing and are capable to growth in the presence of organo-phosphorous pesticide (chlorpyrifos). The mega plasmid size >23 kb was found in MS I and III. Biosurfactants of the significant amount were produced by three isolates. The ability of the isolates to degrade pesticide over 3 days in the presence of pesticides containing chlorpyrifos as the active component was estimated...
May 1, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29750595/long-term-low-frequency-cluster-of-a-german-imipenemase-1-producing-enterobacter-hormaechei-ssp-steigerwaltii-st89-in-a-tertiary-care-hospital-in-germany
#2
Andreas F Wendel, Sebastian Meyer, René Deenen, Karl Köhrer, Susanne Kolbe-Busch, Klaus Pfeffer, Matthias Willmann, Achim J Kaasch, Colin R MacKenzie
Enterobacter cloacae complex is a common cause of hospital outbreaks. A retrospective and prospective molecular analysis of carbapenem-resistant clinical isolates in a tertiary care center demonstrated an outbreak of a German-imipenemase-1 (GIM-1) metallo-beta-lactamase-producing Enterobacter hormaechei ssp. steigerwaltii affecting 23 patients between 2009 and 2016. Thirty-three isolates were sequence type 89 by conventional multilocus sequence typing (MLST) and displayed a maximum difference of 49 out of 3,643 targets in the ad-hoc core-genome MLST (cgMLST) scheme (SeqSphere+ software; Ridom, Münster, Germany)...
May 11, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29737393/a-highly-active-pantothenate-synthetase-from-corynebacterium-glutamicum-enables-the-production-of-d-pantothenic-acid-with-high-productivity
#3
Fitsum Tigu, Junli Zhang, Guoxia Liu, Zhen Cai, Yin Li
D-Pantothenic acid (vitamin B5 ) has wide applications in the feed, food, chemical, and pharmaceutical industries. Its biological production routes which employ pantothenate synthetase (PS) as the key enzyme are attractive since they avoid the tedious and time-consuming optical resolution process. However, little data is available on the activity and kinetics of this enzyme, hampering the rational selection of an efficient enzyme for the biological production of D-pantothenic acid. In this study, six phylogenetically distant PS-encoding genes, from Escherichia coli, Corynebacterium glutamicum, Bacillus subtilis, Bacillus thuringiensis, Bacillus cereus, and Enterobacter cloacae, were expressed in E...
May 8, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29732559/impact-of-poxb-pta-and-acka-genes-on-mineral-weathering-of-enterobacter-cloacae-s71
#4
Qi Wang, Zhendong Zhang, Ying Zhu, Linyan He, Xiafang Sheng
In this study, biotite weathering behaviors were compared between mineral-weathering bacteria Enterobacter cloacae S71, mutant strains created by the deletion of poxB, pta, and ackA genes involved in acetate formation, and their complemented strains. Compared to strain S71, a decrease in bacterial growth was observed during the early and middle stages for the mutant ΔpoxB and at the middle and later stages for the mutants Δpta and ΔackA. Dissolved Al and Fe concentrations were lower during the early stage for strain ΔpoxB, at the early or middle stage for strain Δpta, and at the middle and later stages and throughout the weathering process for strain ΔackA, compared to strain S71...
May 7, 2018: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29726697/antibacterial-activity-of-thymus-vulgaris-essential-oil-alone-and-in-combination-with-cefotaxime-against-bla-esbl-producing-multidrug-resistant-enterobacteriaceae-isolates
#5
Qada Benameur, Teresa Gervasi, Vito Pellizzeri, Mária Pľuchtová, Hassiba Tali-Maama, Farida Assaous, Badia Guettou, Kheira Rahal, Daniela Gruľová, Giacomo Dugo, Andreana Marino, Meriem-Hind Ben-Mahdi
The aim was to evaluate the susceptibility of blaESBL producing Enterobacteriaceae to Slovakian Thymus vulgaris essential oil (TVEO) alone and in combination with cefotaxime (CTX). TVEO composition was determined by gas chromatograph-mass spectrometer (GC/MS). Susceptibility to 21 antibiotics was determined by disc diffusion assay. Genes characterization for resistance to β-lactams was accomplished by polymerase chain reaction (PCR). The antibacterial activity was investigated by standard methods. The synergistic interaction was determined by checkerboard test...
May 4, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29725275/td-gc-ms-analysis-of-volatile-markers-emitted-from-mono-and-co-cultures-of-enterobacter-cloacae-and-pseudomonas-aeruginosa-in-artificial-sputum
#6
Oluwasola Lawal, Hugo Knobel, Hans Weda, Tamara M E Nijsen, Royston Goodacre, Stephen J Fowler
Introduction: Infections such as ventilator-associated pneumonia (VAP) can be caused by one or more pathogens. Current methods for identifying these pathogenic microbes often require invasive sampling, and can be time consuming, due to the requirement for prolonged cultural enrichment along with selective and differential plating steps. This results in delays in diagnosis which in such critically ill patients can have potentially life-threatening consequences. Therefore, a non-invasive and timely diagnostic method is required...
2018: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/29718820/characteristics-of-surgical-site-infection-following-colorectal-surgery-in-a-tertiary-center-extended-spectrum-%C3%AE-lactamase-producing-bacteria-culprits-in-disease
#7
Eliana Kalakouti, Constantinos Simillis, Gianluca Pellino, Nabeela Mughal, Oliver Warren, Sarah Mills, Emile Tan, Christos Kontovounisios, Paris P Tekkis
INTRODUCTION: Surgical site infection (SSI) is a well-known complication of colorectal surgery associated with increased morbidity and hospital stay. Antimicrobial prophylaxis can reduce the risk of SSI by as much as 75%. Extended-spectrum β-lactamase (ESBL)-producing pathogens make the successful use of such prophylaxis a challenge and are a real threat to patient care following colorectal surgery. OBJECTIVE: The aim of this study is to report the common characteristics of SSIs after colorectal surgery and to highlight the prevalence, risk factors, and clinical relevance of ESBL infections among these patients in a tertiary center...
December 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/29716812/first-report-of-the-isolation-of-bla-imi-1-producing-colistin-heteroresistant-enterobacter-cloacae-in-japan-september-2016
#8
Norihito Tarumoto, Masahiro Kodana, Noriyuki Watanabe, Jun Sakai, Kazuo Imai, Toshiyuki Yamaguchi, Yoshitada Taji, Yasuhiro Ebihara, Takashi Murakami, Takuya Maeda, Kotaro Mitsutake, Shigefumi Maesaki
The isolation of a carbapenem-resistant Enterobacter cloacae strain harboring the IMI-1 variant of blaIMI-1 carbapenemase points to the worldwide emergence of multidrug resistant bacteria as a potential source of health care infections. In this report, we describe the first isolation of E. cloacae with blaIMI-1 carbapenemase isolated from a Japanese patient in September 2016. The isolate was resistant to carbapenems, levofloxacin, and aminoglycosides, and heteroresistant to colistin but sensitive to fourth-generation cephalosporins...
April 28, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/29709404/microbiology-of-french-military-casualties-repatriated-from-overseas-for-an-open-traumatic-injury
#9
S Larréché, A Bousquet, C Soler, C Mac Nab, D de Briel, D Delaune, C Bigaillon, P Pasquier, C Dubost, T Demoures, B Malgras, S Ausset, S de Rudnicki, T Leclerc, B de Loynes, S Bonnet, N Mocellin, C Ficko, R Haus, O Hersan, S Rigal, A Mérens
BACKGROUND: This study aimed to describe the microbiological epidemiology of repatriated French soldiers with an open traumatic injury, and to measure the proportion of multidrug-resistant bacteria (MDRB). METHODS: Retrospective study including all French soldiers repatriated in 2011 and 2012 in Parisian military hospitals for open traumatic injury. Results of clinical samples and MDRB screening were collected. The antibiotic susceptibility was assessed using the agar disk diffusion method...
April 27, 2018: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/29698080/superficial-chronic-ulcerative-dermatitis-scud-in-psittacine-birds-review-of-11-cases-2008-2016
#10
Tariq Abou-Zahr, Daniel Calvo Carrasco, Naomi Shimizu Dvm, Neil A Forbes, Thomas A G Dutton, Fiona Froehlich, Filippo De Bellis
We reviewed 11 cases of superficial chronic ulcerative dermatitis (SCUD) in psittacine birds that presented to an exotic animal practice over an 8-year period. African grey parrots ( Psittacus erithacus) were overrepresented, accounting for 55% of SCUD-affected birds. All affected birds were hand-reared pets and bonded strongly to their owners. In all cases, fungal culture results were negative. The most commonly cultured bacteria were Enterobacter cloacae (27%), followed by Escherichia coli, and Staphylococcus aureus (both 18%)...
March 2018: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/29694709/identification-of-n-acyl-homoserine-lactone-degrading-bacteria-isolated-from-rainbow-trout-oncorhynchus-mykiss
#11
Somayeh Torabi Delshad, Siyavash Soltanian, Hassan Sharifiyazdi, Masoud Haghkhah, Peter Bossier
AIMS: A variety of pathogens use quorum sensing (QS) to control the expression of their virulence factors. QS interference has hence been proposed as a promising anti-virulence strategy. The specific aim of this study was to isolate bacteria from trout tissue able to degrade N-Acyl Homoserine Lactones (AHL), a QS molecule family. METHODS AND RESULTS: In total 132 isolates were screened for AHL degradation using Chromobacterium violaceum CV026 as a biosensor. Twenty four quorum quenching (QQ) isolates were identified biochemically and characterized using 16S rDNA sequencing...
April 25, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29686153/-enterobacter-cloacae-complex-isolated-from-shrimps-from-vietnam-encoding-bla-imi-1-resistant-to-carbapenems-but-not-cephalosporins
#12
Michael S M Brouwer, Michel Rapallini, Yvon Geurts, Frank Harders, Alex Bossers, Dik J Mevius, Ben Wit, Kees T Veldman
In August of 2017, a batch of Penaeus monodon (Asian tiger shrimp) and Penaues vannamei (White leg shrimp), originating from fish farms in Vietnam, were screened for the presence of carbapenamase producing Enterobacteriaceae (CPE).….
April 23, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29679645/sequencing-of-pt5282-ctxm-p13190-kpc-and-p30860-nr-and-comparative-genomics-analysis-of-incx8-plasmids
#13
Haihong Fang, Jiao Feng, Yuanhong Xu, Ying Zhang, Zhe Zhan, Zhe Yin, Xiongbo Duan, Bo Gao, Yigang Tong, Jinglin Wang, Yanping Luo, Erhei Dai, Dongsheng Zhou
This study proposed a replicon-based scheme for typing IncX into nine separately clustering subgroups, including IncX1α, IncX1β and IncX2-8. The complete nucleotide sequences of three IncX8 plasmids, namely pT5282-CTXM and p30860-NR from Enterobacter cloacae and p13190-KPC from Klebsiella pneumoniae, were determined and compared with all the other two previously sequenced IncX8 plasmids pCAV1043-58 and pCAV1741-16. These five plasmids possessed conserved IncX8 backbones with limited genetic variations with respect to gene content and organization, and each of them carried one or three accessory modules that harbored resistance markers and metabolic gene clusters as well as transposons, insertion sequence (IS)-based transposition units and miniature inverted repeat transposable elements, indicating that the relatively small IncX8 backbones were able to integrate various foreign genetic contents...
April 18, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29673319/carbapenemase-producing-enterobacteriaceae-in-mexico-report-of-seven-non-clonal-cases-in-a-pediatric-hospital
#14
Alejandra Aquino-Andrade, Jocelin Merida-Vieyra, Eduardo Arias de la Garza, Patricia Arzate-Barbosa, Agustín De Colsa Ranero
BACKGROUND: Carbapenemases-producing Enterobacteriaceae (CPE) are a worldwide public health emergency. In Mexico, reports of CPE are limited, particularly in the pediatric population. Here, we describe the clinical, epidemiological, and molecular characteristics of seven consecutive cases in a third-level pediatric hospital in Mexico City over a four-month period during 2016. RESULTS: The Enterobacteriaceae identified were three Escherichia coli strains (producing OXA-232, NDM-1 and KPC-2), two Klebsiella pneumoniae strains (producing KPC-2 and NDM-1), one Klebsiella oxytoca strain producing OXA-48 and one Enterobacter cloacae strain producing NDM-1...
April 19, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29669108/genomic-context-of-resistance-genes-within-a-french-clinical-mdr-proteus-mirabilis-identification-of-the-novel-genomic-resistance-island-gipmi1
#15
Eliane Siebor, Claire de Curraize, Catherine Neuwirth
Objectives: To determine the location of the antibiotic resistance genes in the MDR Proteus mirabilis PmPHI clinical isolate. Methods: WGS and de novo assembly were performed. BLAST searches were used to identify relevant contigs. PCR and Sanger sequencing were used to link the fragments of interest and fill the gaps. Results: P. mirabilis PmPHI was resistant to six classes of antibiotics: penicillins, aminoglycosides, phenicols, tetracyclines, folate inhibitors and fluoroquinolones...
April 12, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29668034/response-of-formed-biofilm-of-enterobacter-cloacae-klebsiella-oxytoca-and-citrobacter-freundii-to-chlorite-based-disinfectants
#16
Linlin Cai, Huawei Wang, Lijiao Liang, Guangyu Wang, Xinglian Xu, Huhu Wang
Bacterial biofilms formed on equipment surfaces are potential sources of cross-contamination and can be responsible for the spread of bacteria involved in food spoilage, such as some Enterobacteriaceae family members. In this study, the effect of chlorite-based disinfectants, including sodium hypochlorite (SH), chlorine dioxide (CD), strongly acidic electrolyzed water (StAEW), and neutral electrolyzed water (NEW), on inactivation of mono-biofilms of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter freundii was evaluated separately...
April 18, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29666548/inhibitory-effect-of-soy-saponins-on-the-activity-of-%C3%AE-lactamases-including-new-delhi-metallo-%C3%AE-lactamase-1
#17
Hitoshi Horie, Asuka Chiba, Shigeo Wada
β-Lactamase-producing bacteria encode enzymes that inactivate β-lactam antibiotics by catalyzing the hydrolysis of the β-lactam ring. Crude soy saponins were observed to have synergistic effects on the antimicrobial activity of β-lactam antibiotics against β-lactamase-producing Staphylococcus aureus strains. Furthermore, the activities of β-lactamases derived from Enterobacter cloacae , Escherichia coli , and S. aureus were decreased significantly in the presence of crude soy saponins. This inhibitory effect was also observed against the New Delhi metallo-β-lactamase 1 (NDM-1), an enzyme whose activity is not inhibited by the current β-lactamase inhibitors...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29662886/high-prevalence-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-among-clinical-isolates-from-cats-and-dogs-admitted-to-a-veterinary-hospital-in-switzerland
#18
Anna Lena Zogg, Sabrina Simmen, Katrin Zurfluh, Roger Stephan, Sarah N Schmitt, Magdalena Nüesch-Inderbinen
Objectives: This study aimed to identify and characterize extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae among clinical samples of companion animals. Methods: A total of 346 non-duplicate Enterobacteriaceae isolates were collected between 2012 and 2016 from diseased cats ( n  = 115) and dogs ( n  = 231). The presence of bla ESBL , PMQR genes, and the azithromycin resistance gene mph(A) was confirmed by PCR and sequencing of bla genes...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29657432/antibacterial-and-antioxidant-activity-of-juniperus-thurifera-l-leaf-extracts-growing-in-east-of-algeria
#19
Merradi Manel, Heleili Nouzha, Mekari Rim, Mekkaoui Imane, Aouachria Sana, Oucheriah Yasmine, Ayachi Ammar
Aim: This work aimed to evaluate the biological activity of the leaf extracts of Juniperus thurifera L., which is an Algerian endemic tree that belongs to the family of Cupressaceae. Materials and Methods: The plant leaves were extracted in solvents of increasing polarity to obtain different extracts such as methanol, petroleum ether, chloroform, ethyl acetate, and aqueous extracts (MeE, PEE, ChlE, EtAE, and AqE). The antioxidant activity of four extracts (MeE, ChlE, EtAE, and AqE) was assessed by trapping test of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical...
March 2018: Veterinary World
https://www.readbyqxmd.com/read/29642825/-investigation-of-carbapenemase-genes-and-molecular-epidemiology-of-enterobacteriaceae-strains-isolated-between-2010-2014-in-a-university-hospitals
#20
Hüseyin Haydar Kutlu, Ebru Us, Alper Tekeli
The worldwide spread of carbapenemase producing Enterobacteriaceae isolates has become a major threat of public health. This worrisome situation leads the development of new methods for carbapenemase screening, detection, prevention of spread and epidemiological data collection as mandatory. In this study, it was aimed to investigate existence and distribution of carbapenemase-encoding genes (CEGs) among carbapenem-resistant Enterobacteriaceae isolated from various clinical samples in Ankara University Faculty of Medicine, Ibni Sina Hospital, Central Microbiology Laboratory between June 2010-May 2014 and detect their clonal relationship...
January 2018: Mikrobiyoloji Bülteni
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