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Enterobacter cloacae

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https://www.readbyqxmd.com/read/28818912/complete-genome-sequence-of-enterobacter-cloacae-704sk10-an-oxa-48-encoding-wastewater-isolate
#1
Roger Marti, Roger Stephan, Jochen Klumpp, Magdalena Nüesch-Inderbinen, Jörg Hummerjohann, Claudia Bagutti, Katrin Zurfluh
Here we present the complete genome sequence of Enterobacter cloacae 704SK10, a Swiss wastewater isolate encoding an OXA-48 carbapenemase. Assembly resulted in closed sequences of the 4,876,946-bp chromosome, a 111,184-bp IncF plasmid, and an OXA-48-encoding IncL plasmid (63,458 bp) nearly identical to the previously described plasmid pOXA-48.
August 17, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28818892/draft-genome-sequences-of-two-enterobacter-cloacae-subsp-cloacae-strains-isolated-from-australian-hematology-patients-with-bacteremia
#2
Lex E X Leong, David Shaw, Lito Papanicolas, Diana Lagana, Ivan Bastian, Geraint B Rogers
Enterobacter cloacae is a common member of the gut microbiota in healthy individuals. However, it is also an opportunistic pathogen, capable of causing bacteremia. We report the draft genomes of two Enterobacter cloacae subspecies cloacae strains isolated from hematology patients with bacteremia. Both isolates carry genes encoding extended-spectrum β-lactamases.
August 17, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28810849/in-vitro-antiplasmodial-activity-and-prophylactic-potentials-of-extract-and-fractions-of-trema-orientalis-linn-stem-bark
#3
John Oludele Olanlokun, Oluwole Moses David, Anthony Jide Afolayan
BACKGROUND: Trema orientalis (T. orientalis Linn) has been used in the management of malaria in the western part of Nigeria and despite its application in ethnomedicine, there is dearth of scientific evidence to justify the acclaimed prophylactic antimalarial usage of the plant. The aim of this study is to assess the in vitro antiplasmodial cell-free assay and chemopreventive efficacy of the methanol extract of the stem bark of T. orientalis and its fractions as a prophylactic regimen for malaria prevention...
August 15, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28808348/rapid-phenotypic-stress-based-microfluidic-antibiotic-susceptibility-testing-of-gram-negative-clinical-isolates
#4
Maxim Kalashnikov, Marc Mueller, Christine McBeth, Jean C Lee, Jennifer Campbell, Andre Sharon, Alexis F Sauer-Budge
Bacteremia is a life-threatening condition for which antibiotics must be prescribed within hours of clinical diagnosis. Since the current gold standard for bacteremia diagnosis is based on conventional methods developed in the mid-1800s-growth on agar or in broth-identification and susceptibility profiling for both Gram-positive and Gram-negative bacterial species requires at least 48-72 h. Recent advancements in accelerated phenotypic antibiotic susceptibility testing have centered on the microscopic growth analysis of small bacterial populations...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28805537/high-prevalence-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-wild-fish-from-the-mediterranean-sea-in-algeria
#5
Soumia Brahmi, Abdelaziz Touati, Catherine Dunyach-Remy, Albert Sotto, Alix Pantel, Jean-Philippe Lavigne
AIM: We investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae among wild fish from the coast of Bejaia (Algeria) in the Mediterranean Sea. RESULTS: From March 2012 to August 2013, gut and gill samples of wild fish were screened for the presence of ESBL-producing Enterobacteriaceae. Strains were characterized with regard to antibiotic resistance, β-lactamase content, plasmid-mediated quinolone resistance, aminoglycoside resistance genes, and clonality (repetitive sequence-based polymerase chain reaction profiles and multilocus sequence typing)...
August 14, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28792697/the-first-enterobacter-cloacae-co-producing-ndm-and-oxa-48-carbapenemases-and-interhospital-spread-of-oxa-48-and-ndm-producing-klebsiella-pneumoniae-in-turkey
#6
Demet Haciseyitoglu, Aysegul Dokutan, Ayham Abulaila, Fatma Erdem, Yasemin Cag, Serdar Ozer, Zerrin Aktas
BACKGROUND: The aim of this study was to investigate the occurrence of carbapenemase-producing Enterobacteriaceae. METHODS: A total of 54 carbapenem nonsusceptible Enterobacteriaceae (CRE) isolates were recovered from clinical samples sent to the Dr. Lutfi Kirdar Kartal Training and Research Hospital from the period 2011 through 2014. Forty-four isolates were Klebsiella pneumoniae (CRKP) and the other 10 were Enterobacter cloacae (CREC).The isolate identifications and antibiotic sensitivity tests were performed using a Vitek2 automatic system...
July 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28780260/treatment-of-gaseous-volatile-organic-compounds-using-a-rotating-biological-filter
#7
Susant Kumar Padhi, Sharad Gokhale
Rotating biological filter (RBF), which provides higher oxygen mass transfer has been developed for treating gaseous volatile organic compounds (VOCs) such as BTEX (Benzene, toluene, ethylbenzene and xylene) at higher concentrations. The screening of enriched cultures has been done initially to enhance the performance of RBF for treating xylene, toluene and xylene, and BTEX at various loading rates. The removal efficiency of BTEX was maximum (82%), higher than toluene and xylene (79%), and xylene (72%). The presence of xylene enhanced the removal of toluene in the mixture...
July 22, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28780198/antimicrobial-resistance-of-3-types-of-gram-negative-bacteria-isolated-from-hospital-surfaces-and-the-hands-of-health-care-workers
#8
Hui-Ping Wang, Hong-Jiang Zhang, Jie Liu, Qi Dong, Shan Duan, Jun-Qi Ge, Zhong-Hua Wang, Zheng Zhang
BACKGROUND: There has been an increased focus in recent years on antimicrobial resistance of bacteria isolated from clinical samples. However, resistance of bacteria from hospital environments has been less frequently investigated. METHODS: According to hygienic standard for disinfection in hospitals, samples were collected from hospital inanimate surfaces and the hands of health care workers after daily cleaning. An automatic microorganism analyzer was used to identify bacteria and test for antimicrobial susceptibility...
August 2, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28778019/photoinactivation-of-multidrug-resistant-bacteria-by-monomeric-methylene-blue-conjugated-gold-nanoparticles
#9
Shakir Khan, Shahper N Khan, Ramovatar Meena, Ayaz M Dar, Ruchita Pal, Asad U Khan
Multidrug resistant (MDR) bacterial infections have become a severe threat to the community health due to a progressive rise in antibiotic resistance. Nanoparticle-based photodynamic therapy (PDT) is increasingly been adopted as a potential antimicrobial option, yet the cytotoxicity associated with PDT is quite unspecific. Herein, we show Concanavalin-A (ConA) directed dextran capped gold nanoparticles (GNPDEX-ConA) enhanced the efficacy and selectivity of methylene blue (MB) induced killing of multidrug resistant clinical isolates...
July 24, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28768449/recurrent-fuo-due-to-intermittent-enterobacter-cloacae-bacteremias-from-an-infected-pacemaker-lead-diagnosed-by-gallium-scan
#10
Burke A Cunha, Ismail Jimada
Fever of unknown origin (FUO) refers to fevers of ≥101° F that persist for ≥3 weeks and remain undiagnosed after a focused inpatient or outpatient workup. FUO may be due to infectious, malignant/neoplastic, rheumatic/inflammatory, or miscellaneous disorders. Recurrent FUOs are due to the same causes of classical FUOs. Recurrent FUOs may have continuous or intermittent fevers and are particularly difficult to diagnose. With intermittent fever, recurrent FUO diagnostic tests are best obtained during fever episodes...
August 2, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28764775/an-assessment-on-the-role-of-endophytic-microbes-in-the-therapeutic-potential-of-fagonia-indica
#11
Lubna Rahman, Zabta K Shinwari, Irum Iqrar, Lutfur Rahman, Faouzia Tanveer
BACKGROUND: Natural products of animals, plants and microbes are potential source of important chemical compounds, with diverse applications including therapeutics. Endophytic bacteria that are especially associated with medicinal plants presents a reservoir of therapeutic compounds. Fagonia indica has been recently investigated by numerous researchers because of its striking therapeutic potential especially in cancer. It is also reported that endophytes play a vital role in the biosynthesis of various metabolites; therefore we believe that endophytes associated with F...
August 1, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28763905/-analysis-of-drug-resistance-of-acinetobacter-baumannii-in-wound-of-children-with-traffic-injury-and-its-relationship-with-antibiotic-use
#12
S Liu, C Wang, Y X Fu
Objective: To know the drug resistance of Acinetobacter baumannii (AB) in wound of children with traffic injury and its relationship with antibiotic use. Methods: Wound exudate of 226 children with traffic injury admitted to our unit from January 2010 to December 2015 were collected. API bacteria identification panels and fully automatic microbiological identification system were used to identify pathogens. Kirby-Bauer paper disk diffusion method was used to detect the drug resistance of pathogens to 18 antibiotics including amoxycillin/clavulanic acid, piperacillin/tazobactam, and imipenem...
July 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28761643/a-prospective-randomised-trial-of-isolated-pathogens-of-surgical-site-infections-ssi
#13
Konstantinos Alexiou, Ioannis Drikos, Maria Terzopoulou, Nikolaos Sikalias, Argyrios Ioannidis, Nikolaos Economou
BACKGROUND: Every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. The distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. Staph. Aureus, Coag(-) Staphylococci, Enterococcus spp and E. Coli are the main strains appearing. In addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (MRSA, C. Albicans) has been reported...
September 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28757348/draft-genome-sequence-of-an-aminoglycoside-resistant-rmtd-2-producing-enterobacter-cloacae-subsp-cloacae-st-395-in-brazil
#14
Evelin Rodrigues Martins, Maria Fernanda Campagnari Bueno, Gabriela Rodrigues Francisco, Fernanda Modesto Tolentino, Louise Cerdeira, Tiago Casella, Monalisa Nogueira Costa, Carla Cevada, Nilton Lincopan, Doroti de Oliveira Garcia, Mara Corrêa Lelles Nogueira
OBJECTIVES: Enterobacter cloacae has recently emerged as an important agent of nosocomial infections, due to the diffusion of extended spectrum β-lactamases and carbapenemases in this species. In this context, a raise in the use of aminoglycosides for therapy was noticed, being followed by the accelerated development of resistance mechanisms. In this study, we report the draft genome sequence of a multidrug-resistant Enterobacter cloacae subsp. cloacae strain (Ec2), isolated from active surveillance culture of a patient with Chagas disease...
July 27, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28754999/identification-of-an-operon-involved-in-fluoride-resistance-in-enterobacter-cloacae-frm
#15
Xiaoqing Liu, Jian Tian, Lihui Liu, Tao Zhu, Xiaoxia Yu, Xiaoyu Chu, Bin Yao, Ningfeng Wu, Yunliu Fan
Fluorine is ubiquitous and the most active non-metal element in nature. While many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are incompletely understood. In this study, a fluoride-resistant strain, Enterobacter cloacae FRM, was identified which could grow well at a fluoride concentration of 4,000 mg/L. According to comparative genomics, transcriptome under fluoride stress, and sequence analyses of two fluoride-resistant fosmid clones, the genomic island GI3 was found to be important for fluoride resistance...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28748087/antimicrobial-effects-of-lysophosphatidylcholine-on-methicillin-resistant-staphylococcus-aureus
#16
Haruko Miyazaki, Naoko Midorikawa, Saki Fujimoto, Natsumi Miyoshi, Hideto Yoshida, Tetsuya Matsumoto
OBJECTIVES: Methicillin-resistant Staphylococcus aureus (MRSA) is an important health care-associated and community-associated pathogen and causes a large number of infections worldwide. For the purpose of application to topical treatment of MRSA infection, we examined the antimicrobial effects of lysophosphatidylcholine (LPC) on MRSA strains. We also investigated the combination effect of LPC and gentamicin on MRSA growth. METHODS: The LPC minimum inhibitory concentrations (MIC) for Gram-positive (S...
July 2017: Therapeutic Advances in Infectious Disease
https://www.readbyqxmd.com/read/28747375/evaluation-of-the-rapid-polymyxin-np-test-for-polymyxin-b-resistance-detection-using-enterobacter-cloacae-and-enterobacter-aerogenes-isolates
#17
Shelby Simar, Diane Sibley, Deborah Ashcraft, George Pankey
Background: Polymyxin resistance is an increasing problem worldwide. Currently, determining susceptibility to polymyxins is problematic and lengthy. Polymyxins diffuse poorly into agar, potentially giving inaccurate disk diffusion and Etest results. A rapid screening test (2 h) for detection of polymyxin-resistance in Enterobacteriaceae, developed by Nordmann and Poirel (Rapid Polymyxin NP Test, 2016), detects glucose metabolization in the presence of polymyxins E and B via pH-induced color change. Sensitivity/specificity were 99...
July 26, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28746900/in-vivo-electrochemical-monitoring-of-h2o2-production-induced-by-root-inoculated-endophytic-bacteria-in-agave-tequilana-leaves
#18
Alex S Lima, Kátia R Prieto, Carla S Santos, Hellen Paula Valerio, Evelyn Y Garcia-Ochoa, Aurora Huerta-Robles, Miguel J Beltran-Garcia, Paolo Di Mascio, Mauro Bertotti
A dual-function platinum disc microelectrode sensor was used for in-situ monitoring of H2O2 produced in A. tequilana leaves after inoculation of their endophytic bacteria (Enterobacter cloacae). Voltammetric experiments were carried out from 0.0 to -1.0V, a potential range where H2O2 is electrochemically reduced. A needle was used to create a small cavity in the upper epidermis of A. tequilana leaves, where the fabricated electrochemical sensor was inserted by using a manual three-dimensional micropositioner...
July 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28743314/multidrug-resistant-bacteria-are-sensitive-to-euphorbia-prostrata-and-six-others-cameroonian-medicinal-plants-extracts
#19
Igor K Voukeng, Veronique P Beng, Victor Kuete
BACKGROUND: Multidrug resistant (MDR) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them. METHODS: Eleven methanol extracts from seven Cameroonian medicinal plants were tested for their antibacterial activity using broth micro-dilution method against 36 MDR bacterial strains including Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosa and Staphylococcus aureus...
July 25, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28739428/novel-class-1-integrons-and-sequence-types-in-vim-2-and-vim-11-producing-clinical-strains-of-enterobacter-cloacae
#20
D De Belder, D Faccone, N Tijet, R G Melano, M Rapoport, A Petroni, C Lucero, F Pasteran, A Corso, S A Gomez
All VIM-producing Enterobacteriaceae (six Enterobacter cloacae) submitted to the Argentinian Reference Laboratory in Antimicrobial Resistance in the period 2008-13 were characterized. The isolates were referred from 6 nosocomial institutions located in 5 different cities across the country. All isolates showed carbapenem disk diffusion inhibition zones ≤22mm and synergism between a carbapenem disk and EDTA/SMA. The six isolates were PCR positive for blaVIM. Imipenem MICs were ≤1 to 8μg/ml. Typing by PFGE and MLST distinguished six pulsotypes and sequence types with blaVIM located on novel class 1 integron arrays: ECL-1: ST182, In883; ECL-2, ST90, In885; ECL-3, ST88, In346 with blaVIM-11; ECL-4, ST184, In900; ECL-5, ST749-new, In900; ECL-6, ST91 and uncharacterized In...
July 21, 2017: Infection, Genetics and Evolution
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