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Enterobacter cloacae

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https://www.readbyqxmd.com/read/28545899/multiple-brain-abscesses-due-to-enterobacter-cloacae-in-an-immune-competent-child
#1
Arushi Gahlot Saini, Vinay Rathore, Chirag Kumar Ahuja, Rajesh Chhabra, Pankaj C Vaidya, Pratibha Singhi
Brain abscesses due to Enterobacteriaceae in immune-competent children are rare, and those due to Enterobacter cloacae are even rarer. We report an interesting case of community-acquired E. cloacae neuroinfection resulting in multiple brain abscesses in a young child with no underlying risk-factors. A 10 year-old-boy presented with low-grade fever, headache, neck pain and progressive deterioration of sensorium. On examination, he was conscious but drowsy with photophobia, normal fundii, meningeal signs, mild hypertonia, brisk muscle stretch reflexes and extensor plantar responses...
May 22, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28540152/epidemiological-clinical-and-microbiological-characteristics-of-patients-with-post-traumatic-osteomyelitis-of-limb-fractures-in-southwest-china-a-hospital-based-study
#2
Jiachen Peng, Youliang Ren, Wenbin He, Zhengdao Li, Jin Yang, Yi Liu, Zhonghui Zheng, Stephen L Kates, Edward M Schwarz, Chao Xie, Youjia Xu
Objective To determine the epidemiological, clinical and microbiological characteristics, of patients with post-traumatic osteomyelitis of extremity fractures, and provide evidence-based guidelines for early diagnosis and treatment, including empiric antibiotic therapy. Methods Human subject research was performed using institutional review board approved protocols. A retrospective chart review was conducted on 5,368 patients diagnosed with extremity traumatic fractures from January 1, 2012 to December 31, 2015, to identify osteomyelitis patients...
2017: J Bone Jt Infect
https://www.readbyqxmd.com/read/28535747/in-vitro-assessment-of-pediococcus-acidilactici-kp10-for-its-potential-use-in-the-food-industry
#3
Sahar Abbasiliasi, Joo Shun Tan, Fatemeh Bashokouh, Tengku Azmi Tengku Ibrahim, Shuhaimi Mustafa, Faezeh Vakhshiteh, Subhashini Sivasamboo, Arbakariya B Ariff
BACKGROUND: Selection of a microbial strain for the incorporation into food products requires in vitro and in vivo evaluations. A bacteriocin-producing lactic acid bacterium (LAB), Pediococcus acidilactici Kp10, isolated from a traditional dried curd was assessed in vitro for its beneficial properties as a potential probiotic and starter culture. The inhibitory spectra of the bacterial strain against different gram-positive and gram-negative bacteria, its cell surface hydrophobicity and resistance to phenol, its haemolytic, amylolytic and proteolytic activities, ability to produce acid and coagulate milk together with its enzymatic characteristics and adhesion property were all evaluated in vitro...
May 23, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28535609/synthesis-of-%C3%AE-galactooligosaccharide-using-bifidobacterial-%C3%AE-galactosidase-purified-from-recombinant-escherichia-coli
#4
So Young Oh, So Youn Youn, Myung Soo Park, Hyoung Geun Kim, Nam In Baek, Zhi Peng Li, Geun Eog Ji
Galactooligosaccharides (GOS) are known to be selectively utilized by Bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. In this study, β-GOS are synthesized using bifidobacterial β-galactosidase(G1) purified from recombinant E. coli with a high GOS yield and with high productivity and enhanced bifidogenic activity. The purified recombinant G1 showed maximum production of β-GOS at pH 8.5 and 45°C. A matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of the major peaks of the produced β-GOS showed MW of 527 and 689, indicating the synthesis of β-GOS at degrees of polymerization (DP) of 3 and DP4, respectively...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28520983/genomic-epidemiology-of-global-vim-producing-enterobacteriaceae
#5
Yasufumi Matsumura, Gisele Peirano, Rebekah Devinney, Patricia A Bradford, Mary R Motyl, Mark D Adams, Liang Chen, Barry Kreiswirth, Johann D D Pitout
Background: : International data on the molecular epidemiology of Enterobacteriaceae with VIM carbapenemases are limited. Methods: We performed short read (Illumina) WGS on a global collection of 89 VIM-producing clinical Enterobacteriaceae (2008-14). Results: VIM-producing (11 varieties within 21 different integrons) isolates were mostly obtained from Europe. Certain integrons with bla VIM were specific to a country in different species and clonal complexes (CCs) (In 87 , In 624 , In 916 and In 1323 ), while others had spread globally among various Enterobacteriaceae species (In 110 and In 1209 )...
May 16, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28509939/bioremoval-of-lead-using-pennisetum-purpureum-augmented-with-enterobacter-cloacae-vitpasj1-a-pot-culture-approach
#6
Anamika Das, Priyanka Belgaonkar, Aditya S Raman, Sofia Banu, Jabez W Osborne
Lead is a toxic heavy metal discharged into the ecosystem from various industries. Biological remediation strategies have been effective in the bioremoval of lead. In our current study, a phytobacterial system using Pennisetum purpureum along with lead-resistant bacterium (LRB) was employed for the uptake of lead. The LRB was obtained from lead-contaminated sites. The isolate VITPASJ1 was found to be highly tolerant to lead and was identified as an effective plant growth-promoting bacterium. The 16S rRNA sequencing revealed VITPASJ1 to be the closest neighbour of Enterobacter cloacae...
May 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28508092/a-metabonomic-analysis-on-the-response-of-enterobacter-cloacae-from-coastal-outfall-for-land-based-pollutant-under-phoxim-stress
#7
Dijun Zhang, Shan He, Tinghong Ming, Chenyang Lu, Jun Zhou, Xiurong Su
Enterobacter cloacae is an opportunistic pathogen widely distributed in human and animal intestinal systems. The secretion of extended-spectrum β-lactamases (ESBLs) and cephalosporinase (AmpC) endows E. cloacae with strong drug resistance. In a previous study by our group, protein expression of E. cloacae under phoxim stress was measured by two-dimensional electrophoresis. Here, nuclear magnetic resonance was used to detect differences in E. cloacae metabonomics when under phoxim stress. We determined that there are 29 types of metabolites that differ between phoxim stress and normal culture conditions...
May 15, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28507488/microbiological-analysis-of-acute-infections-of-the-nail-fold-on-the-basis-of-bait-thread-test
#8
Hanna Tomczak, Aleksandra Dańczak-Pazdrowska, Adriana Polańska, Agnieszka Osmola-Mańkowska, Jakub Pazdrowski, Wioleta Błażejewska-Gąsior, Anna Horla, Marta Hasse-Cieślińska, Zygmunt Adamski
INTRODUCTION: An acute infection of the nail fold, called paronychia, is a common clinical problem. The basis for the implementation of the treatment is the result of microbiological examination. Due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. AIM: The microbial analysis of acute infections of the nail fold. MATERIAL AND METHODS: The study included 32 tests conducted on 31 patients of the Department of Dermatology...
April 2017: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/28491136/applications-of-microalgal-biofilms-for-wastewater-treatment-and-bioenergy-production
#9
Ana F Miranda, Narasimhan Ramkumar, Constandino Andriotis, Thorben Höltkemeier, Aneela Yasmin, Simone Rochfort, Donald Wlodkowic, Paul Morrison, Felicity Roddick, German Spangenberg, Banwari Lal, Sanjukta Subudhi, Aidyn Mouradov
BACKGROUND: Microalgae have shown clear advantages for the production of biofuels compared with energy crops. Apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (C, N, and P), heavy metals and micropollutants, and they do not compete with crops for arable lands. However, fundamental barriers to the industrial application of microalgae for biofuel production still include high costs of removing the algae from the water and the water from the algae which can account for up to 30-40% of the total cost of biodiesel production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28491055/metagenomic-sequencing-of-diamondback-moth-gut-microbiome-unveils-key-holobiont-adaptations-for-herbivory
#10
Xiaofeng Xia, Geoff M Gurr, Liette Vasseur, Dandan Zheng, Huanzi Zhong, Bingcai Qin, Junhan Lin, Yue Wang, FengQin Song, Yong Li, Hailan Lin, Minsheng You
Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. Here we expand on that work with a complete taxonomic and functional profile of the P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28476242/evaluation-of-the-carbapenem-inactivation-method-cim-for-detecting-carbapenemase-activity-in-enterobacteria
#11
A Aguirre-Quiñonero, M E Cano, D Gamal, J Calvo, L Martínez-Martínez
OBJECTIVES: The objective of this study was to evaluate the accuracy of the CIM test in the detection of carbapenemase activity in 124 strains of Enterobacteriaceae. METHODS: A panel of 124 previously characterized Enterobacteriaceae was tested: 77 strains producing the following carbapenemase families: KPC (n = 14), GES (n = 22), NDM (n = 19), VIM (n = 4), IMP (n = 4) and OXA-48 (n = 14) and 47 non-carbapenemase producers. For the CIM method, an active susceptibility meropenem disc was exposed to a bacterial suspension of a test strain; when a carbapenemase is produced, the antibiotic is inactivated allowing uninhibited growth of an indicator strain after overnight incubation...
March 16, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28475792/clinical-and-molecular-epidemiology-of-carbapenem-resistant-enterobacteriaceae-among-adult-inpatients-in-singapore
#12
Kalisvar Marimuthu, Indumathi Venkatachalam, Wei Xin Khong, Tse Hsien Koh, Benjamin Pei Zhi Cherng, My Van La, Partha Pratim De, Prabha Unny Krishnan, Thean Yen Tan, Raymond Fong Kok Choon, Surinder Kaur Pada, Choong Weng Lam, Say Tat Ooi, Rama Narayana Deepak, Nares Smitasin, Eng Lee Tan, Jia Jun Lee, Asok Kurup, Barnaby Young, Nancy Tee Wen Sim, Koh Cheng Thoon, Dale Fisher, Moi Lin Ling, Brenda Ang Sze Peng, Yik-Ying Teo, Li Yang Hsu, Raymond Tzer Pin Lin, Rick Twee-Hee Ong, Jeanette Teo, Oon Tek Ng
Background: Since 2010, the incidence of carbapenem-resistant Enterobacteriaceae (CRE) has been increasing in Singapore. We analyzed the clinical and molecular epidemiology of CRE among adult inpatients in Singapore. Methods: Quarterly incidence of unique subjects (per 100000 patient-days) with positive clinical and surveillance cultures for CRE were estimated based on mandatory data submitted to the National Public Health Laboratory by public hospitals between 2010 and 2015...
May 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28458566/usefulness-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-to-identify-pathogens-including-polymicrobial-samples-directly-from-blood-culture-broths
#13
Maya Hariu, Yuji Watanabe, Nozomi Oikawa, Masafumi Seki
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (TOF-MS) is now widely used to detect pathogens in clinical settings in Japan. Here, we report the ability of TOF-MS to detect bacteria from blood culture (BC) broths, and compare the efficacy of TOF-MS to that of conventional culture methods. Bacteria were correctly detected from 63 monomicrobial samples within 80 minutes; results matched those obtained by conventional BC methods, although the conventional methods took 2-3 days. In addition to the 63 monomicrobial samples, another three polymicrobial samples were tested; notably, the infecting bacteria were not correctly identified in two of these three samples...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28449650/structure-genetics-and-worldwide-spread-of-new-delhi-metallo-%C3%AE-lactamase-ndm-a-threat-to-public-health
#14
REVIEW
Asad U Khan, Lubna Maryam, Raffaele Zarrilli
BACKGROUND: The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. MAIN BODY: In this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species...
April 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28442305/transfer-of-kpc-2-carbapenemase-from-klebsiella-pneumoniae-to-enterobacter-cloacae-in-a-patient-receiving-meropenem-therapy
#15
Evelin Rodrigues Martins, Cássia Fernanda Estofolete, Andressa Batista Zequini, Louise Cerdeira, Doroti de Oliveira Garcia, Maria Fernanda Campagnari Bueno, Gabriela Rodrigues Francisco, Leonardo Neves de Andrade, Ana Lúcia da Costa Darini, Fernanda Modesto Tolentino, Tiago Casella, Nilton Lincopan, Mara Corrêa Lelles Nogueira
The horizontal transfer of a plasmid bearing the blaKPC-2 gene from K. pneumoniae to E. cloacae infecting the respiratory tract of a patient during meropenem therapy was elucidated. This finding is particularly worrisome, since these drugs are of last resort for multidrug-resistant Gram-negative pathogens.
April 12, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28434982/novel-monoclonal-antibodies-against-stx1d-and-1e-and-their-use-for-improving-immunoassays
#16
Xiaohua He, Stephanie Patfield, Reuven Rasooly, Daniela Mavrici
Shiga toxins (Stxs) are major causative agents for bloody diarrhea and hemolytic uremic syndrome, a life-threatening disease in humans. No effective treatment is available. Early detection of Stxs in clinical samples is critical for disease management. As bacteria evolve, new Stxs are produced; therefore, methods used to identify them need to be improved as well. In this study, new monoclonal antibodies (mAbs) against Stx1d and 1e were developed and used to improve a commercial Stx1 kit. Incorporation of the new mAbs into the Abraxis Stx1 kit not only increased the assay sensitivity to Stx1d, but the assay was conferred the ability to detect Stx1e, a newly identified subtype of Stx1 produced by an atypical Stx-producing bacterial strain, Enterobacter cloacae M12X01451, isolated from a clinical specimen...
April 20, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28428984/a-comparison-of-molecular-typing-methods-applied-to-enterobacter-cloacae-complex-hsp60-sequencing-rep-pcr-and-mlst
#17
Roberto A Viau, Lee M Kiedrowski, Barry N Kreiswirth, Mark Adams, Federico Perez, Dror Marchaim, Dubert M Guerrero, Keith S Kaye, Latania K Logan, Maria Virginia Villegas, Robert A Bonomo
Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST)...
2017: Pathogens & Immunity
https://www.readbyqxmd.com/read/28419869/allodemic-distribution-of-plasmids-co-harbouring-blactx-m-15-aac-6-ib-cr-qnrb-in-klebsiella-pneumoniae-is-the-main-source-of-extended-spectrum-%C3%AE-lactamases-in-uruguay-s-paediatric-hospital
#18
V Garcia-Fulgueiras, L Araujo, I Bado, N F Cordeiro, M I Mota, G Laguna, G Algorta, R Vignoli
OBJECTIVES: This study aimed to describe the characteristics of clinical isolates of extended-spectrum β-lactamase (ESBL)-producing enterobacteria (EPE) in Uruguay's paediatric hospital. METHODS: ESBLs, qnr alleles and aac(6')-Ib-cr were sought and characterised in EPE isolated between March 2010 and March 2012. Transfer of resistance determinants was assessed by conjugation. Incompatibility (Inc) groups, plasmid toxin-antitoxin systems (TAS) and plasmid size were determined in transconjugants...
April 15, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28418318/carbapenem-resistant-enterobacter-cloacae-in-patients-from-the-us-veterans-health-administration-2006-2015
#19
Brigid M Wilson, Nadim G El Chakhtoura, Sachin Patel, Elie Saade, Curtis J Donskey, Robert A Bonomo, Federico Perez
We analyzed carbapenem-resistant Enterobacteriaceae (CRE) trends among patients from the US Veterans Health Administration (VHA). After the emergence of CRE in the eastern United States, resistance rates remained stable in Klebsiella pneumoniae but increased in Enterobacter cloacae complex, suggesting a "second epidemic". VHA offers a vantage point for monitoring nationwide CRE trends.
May 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28414678/what-the-eyes-don-t-see-the-heart-does-grieve-over
#20
M Sciaudone, M Dery
INTRODUCTION: A 71 year old Peruvian woman presented with blurry vision and bilateral eye pain and had evidence of bilateral endophthalmitis on eye examination. Her past medical history was notable for multiple abdominal surgeries within the preceding months, including an incarcerated inguinal hernia repair which required an ileostomy placement, and cholecystitis requiring cholecystostomy tube placement. Over her multiple long hospitalizations, she developed bacteremia and fungemia on several occasions, with organisms including Enterobacter cloacae, Klebsiella pneumoniae, Bacteroides fragilis, Pseudomonas aeruginosa, Enterococcus faecium, and Candida albicans...
March 2017: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
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