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https://www.readbyqxmd.com/read/27890667/investigation-of-carbapenem-resistance-and-the-first-identification-of-klebsiella-pneumoniae-carbapenemase-kpc-enzyme-among-escherichia-coli-isolates-in-turkey-a-prospective-study
#1
Mert Ahmet Kuskucu, Asiye Karakullukcu, Mailihaba Ailiken, Barıs Otlu, Bilgul Mete, Gokhan Aygun
BACKGROUND: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coli isolates from clinical samples in Turkey. METHODS: The prospective study included a total of 4052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed-field gel electrophoresis (PFGE), respectively...
November 23, 2016: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/27825605/antimicrobial-resistance-in-enterobacteriaceae-in-brazil-focus-on-%C3%AE-lactams-and-polymyxins
#2
REVIEW
Jorge Luiz Mello Sampaio, Ana Cristina Gales
During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96...
October 25, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27812159/enterobacteriaceae-isolated-from-the-river-danube-antibiotic-resistances-with-a-focus-on-the-presence-of-esbl-and-carbapenemases
#3
Clemens Kittinger, Michaela Lipp, Bettina Folli, Alexander Kirschner, Rita Baumert, Herbert Galler, Andrea J Grisold, Josefa Luxner, Melanie Weissenbacher, Andreas H Farnleitner, Gernot Zarfel
In a clinical setting it seems to be normal these days that a relevant proportion or even the majority of different bacterial species has already one or more acquired antibiotic resistances. Unfortunately, the overuse of antibiotics for livestock breeding and medicine has also altered the wild-type resistance profiles of many bacterial species in different environmental settings. As a matter of fact, getting in contact with resistant bacteria is no longer restricted to hospitals. Beside food and food production, the aquatic environment might also play an important role as reservoir and carrier...
2016: PloS One
https://www.readbyqxmd.com/read/27811089/draft-genome-sequence-of-a-hospital-associated-clone-of-klebsiella-pneumoniae-st340-cc258-coproducing-rmtg-and-kpc-2-isolated-from-a-pediatric-patient
#4
Louise Cerdeira, Miriam R Fernandes, Gabriela R Francisco, Maria Fernanda C Bueno, Susan Ienne, Tiago A Souza, Doroti de Oliveira Garcia, Nilton Lincopan
We report here the draft genome sequence of a Klebsiella pneumoniae strain 1194/11, belonging to the hospital-associated sequence type 340 (ST340; clonal complex CC258), isolated from a catheter tip culture from a pediatric patient. The multidrug-resistant strain coproduced the 16S rRNA methyltransferase rRNA RmtG and β-lactamases KPC-2 and CTX-M-15.
November 3, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27770796/a-cluster-of-kpc-2-and-vim-2-producing-klebsiella-pneumoniae-st833-isolates-from-the-pediatric-service-of-a-venezuelan-hospital
#5
Aura Falco, Yusibeska Ramos, Esther Franco, Alegría Guzmán, Howard Takiff
BACKGROUND: Klebsiella pneumoniae is a bacterial pathogen that has developed resistance to multiple antibiotics and is a major cause of nosocomial infections worldwide. Carbapenemase-producing Klebsiella pneumoniae have been isolated in many hospitals in Venezuela, but they have not been well-studied. The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae isolates from the pediatric service of a hospital located in Anzoategui State, in the eastern part of Venezuela...
October 22, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27756125/engineering-specificity-from-broad-to-narrow-design-of-a-blip-variant-that-exclusively-binds-and-detects-kpc-%C3%AE-lactamase
#6
Dar-Chone Chow, Kacie Rice, Wanzhi Huang, Robert L Atmar, Timothy G Palzkill
The β-lactamase inhibitory protein (BLIP) binds and inhibits a wide range of class A β-lactamases including the TEM-1 β-lactamase (Ki= 0.5 nM), which is widely present in Gram-negative bacteria, and the KPC-2 β-lactamase (Ki= 1.2 nM), which hydrolyzes virtually all clinically useful β-lactam antibiotics. The extent to which the specificity of a protein that binds a broad range of targets can be modified to display narrow specificity was explored in this study by engineering BLIP to bind selectively to KPC-2 β-lactamase...
October 18, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27754776/first-description-of-kpc-2-producing-escherichia-coli-and-st15-oxa-48-positive-klebsiella-pneumoniae-in-tunisia
#7
Farah Ben Tanfous, Carla Andrea Alonso, Wafa Achour, Laura Ruiz-Ripa, Carmen Torres, Assia Ben Hassen
The aim of this study was to investigate the molecular features among Klebsiella pneumoniae and Escherichia coli strains showing a resistant/intermediate-resistant phenotype to ertapenem (R/IR-ERT), implicated in colonization/infection in patients of the Hematology and Graft Units of the National Bone Marrow Transplant Center of Tunisia (3-year period, 2011-2014). The major carbapenemase, extended-spectrum beta-lactamase, and plasmidic AmpC beta-lactamase genes were analyzed and characterized by PCR and sequencing...
October 18, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27733128/nosocomial-outbreak-of-kpc-2-and-ndm-1-producing-klebsiella-pneumoniae-in-a-neonatal-ward-a-retrospective-study
#8
Jing Yu, Kun Tan, Zhihui Rong, Yue Wang, Zhongju Chen, Xuhui Zhu, Li Wu, Li Tan, Wei Xiong, Ziyong Sun, Ling Chen
BACKGROUND: The spread of resistance to carbapenems among Enterobacteriaceae has become a major public health problem in recent years. In this study, we describe an outbreak of Klebsiella pneumoniae in the neonatal ward. First, we aimed to study the drug resistance, genetic relatedness, and transmission mechanism of carbapenem-resistant K. pneumoniae; second, we implemented infection control measures to contain the outbreak. METHODS: We investigated 27 non-repetitive strains isolated from neonates and five strains cultured from around the neonatal ward...
October 12, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27691984/increase-in-prevalence-of-kpc-2-producing-klebsiella-pneumoniae-recovered-from-respiratory-secretions-of-intensive-care-patients-getting-a-free-ride-on-a-menacing-colistin-resistance
#9
Leandro Reus Rodrigues Perez
No abstract text is available yet for this article.
December 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27632226/structure-based-approach-for-identification-of-novel-phenylboronic-acids-as-serine-%C3%AE-lactamase-inhibitors
#10
Jacopo Sgrignani, Filomena De Luca, Hayarpi Torosyan, Jean-Denis Docquier, Da Duan, Beatrice Novati, Fabio Prati, Giorgio Colombo, Giovanni Grazioso
β-Lactamases are bacterial enzymes conferring resistance to β-lactam antibiotics in clinically-relevant pathogens, and represent relevant drug targets. Recently, the identification of new boronic acids (i.e. RPX7009) paved the way to the clinical application of these molecules as potential drugs. Here, we screened in silico a library of ~1400 boronic acids as potential AmpC β-lactamase inhibitors. Six of the most promising candidates were evaluated in biochemical assays leading to the identification of potent inhibitors of clinically-relevant β-lactamases like AmpC, KPC-2 and CTX-M-15...
October 2016: Journal of Computer-aided Molecular Design
https://www.readbyqxmd.com/read/27591293/low-biological-cost-of-carbapenemase-encoding-plasmids-following-transfer-from-klebsiella-pneumoniae-to-escherichia-coli
#11
Maria Chiara Di Luca, Vidar Sørum, Irina Starikova, Julia Kloos, Nils Hülter, Umaer Naseer, Pål J Johnsen, Ørjan Samuelsen
OBJECTIVES: The objective of this study was to determine the biological cost, stability and sequence of two carbapenemase-encoding plasmids containing blaKPC-2 (pG12-KPC-2) and blaVIM-1 (pG06-VIM-1) isolated from Klebsiella pneumoniae when newly acquired by uropathogenic Escherichia coli clinical isolates of different genetic backgrounds. METHODS: The two plasmids were transferred into plasmid-free E. coli clinical isolates by transformation. The fitness effect of newly acquired plasmids on the host cell was assessed in head-to-head competitions with the corresponding isogenic strain...
September 2, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27585787/high-minimum-inhibitory-concentration-of-imipenem-as-a-predictor-of-fatal-outcome-in-patients-with-carbapenem-non-susceptible-klebsiella-pneumoniae
#12
Ping-Feng Wu, Chien Chuang, Chin-Fang Su, Yi-Tsung Lin, Yu-Jiun Chan, Fu-Der Wang, Yin-Ching Chuang, L Kristopher Siu, Chang-Phone Fung
Carbapenem resistance in Klebsiella pneumoniae is important because of its increasing prevalence and limited therapeutic options. To investigate the clinical and microbiological characteristics of patients infected or colonized with carbapenem non-susceptible K. pneumoniae (CnsKP) in Taiwan, we conducted a retrospective study at Taipei Veterans General Hospital from January 2012 to November 2013. Carbapenem non-susceptibility was defined as a minimum inhibitory concentration (MIC) of ≥2 mg/L for imipenem or meropenem...
September 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27580572/antimicrobial-susceptibility-and-molecular-epidemiology-of-multidrug-resistant-klebsiella-pneumoniae-in-central-china
#13
Xiaobing Guo, Zaiqiu Cao, Zhifeng Dai, Yuan Li, Xiaohong He, Xiaoxin Hu, Fuyun Tian, Yihui Ren
Extended-spectrum-beta-lactamase-producing and carbapenemase-producing Klebsiella pneumoniae has rapidly spread through clinical units worldwide. This study investigated the epidemiology and resistance profiles of K. pneumoniae strains isolated in Central China between 2009 and 2014. Antimicrobial susceptibility tests and polymerase chain reaction were used to investigate the prevalence and resistance of ESBLs and carbapenemases produced by K. pneumoniae, and the prevalence of KPC-producing K. pneumoniae was investigated by multilocus sequence typing...
August 31, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27578505/prevalence-and-molecular-characteristics-of-carbapenemase-producing-enterobacteriaceae-from-five-hospitals-in-korea
#14
Seok Hoon Jeong, Han Sung Kim, Jae Seok Kim, Dong Hoon Shin, Hyun Soo Kim, Min Jeong Park, Saeam Shin, Jun Sung Hong, Seung Soon Lee, Wonkeun Song
BACKGROUND: The emergence of carbapenemase-producing Enterobacteriaceae (CPE) represents a major clinical problem because these bacteria are resistant to most antibiotics. CPE remain relatively uncommon in Korea. We report the prevalence, clinical characteristics, and molecular epidemiology of CPE isolates collected from five university hospitals in Korea. METHODS: Between January and December 2015, 393 non-duplicated isolates that were nonsusceptible to ertapenem were analyzed...
November 2016: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/27531996/outbreak-by-ventilator-associated-st11-k-pneumoniae-with-co-production-of-ctx-m-24-and-kpc-2-in-a-sicu-of-a-tertiary-teaching-hospital-in-central-china
#15
Longhua Hu, Yanling Liu, Linqiang Deng, Qiaoshi Zhong, Yaping Hang, Zengzeng Wang, Lingling Zhan, Liangxing Wang, Fangyou Yu
The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) often responsible for numerous hospital-associated outbreaks has become an important public health problem. From January 2013 to February 2014, a total of 41 non-duplicate K. pneumoniae isolates with carbapenem resistance, were collected at a tertiary teaching hospital in Nanchang, central China. Among 41 K. pneumoniae isolates, 28 were isolated from hospitalized patients including 19 from the patients in surgery intensive care unit (SICU) and 13 were isolated from ventilators...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27530752/the-spread-of-kpc-producing-enterobacteriaceae-in-spain-wgs-analysis-of-the-emerging-high-risk-clones-of-klebsiella-pneumoniae-st11-kpc-2-st101-kpc-2-and-st512-kpc-3
#16
Jesús Oteo, María Pérez-Vázquez, Verónica Bautista, Adriana Ortega, Pilar Zamarrón, David Saez, Sara Fernández-Romero, Noelia Lara, Raquel Ramiro, Belén Aracil, José Campos
OBJECTIVES: We analysed the microbiological traits and population structure of KPC-producing Enterobacteriaceae isolates collected in Spain between 2012 and 2014. We also performed a comparative WGS analysis of the three major KPC-producing Klebsiella pneumoniae clones detected. METHODS: Carbapenemase and ESBL genes were sequenced. The Institut Pasteur MLST scheme was used. WGS data were used to construct phylogenetic trees, to identify the determinants of resistance and to de novo assemble the genome of one representative isolate of each of the three major K...
August 15, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27477421/bloodstream-infections-caused-by-klebsiella-pneumoniae-carbapenemase-2-producing-k-pneumoniae-at-a-hematology-department-in-wenzhou-china
#17
Chongyun Xing, Bei Ge, Kang Yu, Shenmeng Gao, Bin Liang, Haige Ye
The increasing prevalence of Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae (KPC-2-KP) infections can become a new life-threatening complication for hematological patients. Five cases of KPC-2-KP bloodstream infections have been identified in our hematology department over the past 10 years. The current treatment options do not show satisfactory efficacy, especially for bloodstream infections. The treatment of these five cases was unsuccessful, mainly due to the high minimum inhibitory concentrations of carbapenem, fosfomycin resistance, or the inaccessibility of polymyxin...
2016: Internal Medicine
https://www.readbyqxmd.com/read/27469416/-rapid-increase-in-resistance-to-third-generation-cephalosporins-imipenem-and-co-resistance-in-klebsiella-pneumoniae-from-isolated-from-7-140-blood-cultures-2010-2014-using-ears-net-data-in-spain
#18
Belén Aracil-García, Jesús Oteo-Iglesias, Óscar Cuevas-Lobato, Noelia Lara-Fuella, Isabel Pérez-Grajera, Sara Fernández-Romero, María Pérez-Vázquez, José Campos
INTRODUCTION: An analysis was made about the evolution of resistance to 3rd generation cephalosporins, imipenem, and other antibiotics in invasive isolates of Klebsiella pneumoniae (K. pneumoniae) according to the Spanish EARS-Net database (2010-2014). METHODS: Forty-two hospitals from 16 Autonomous Communities with an approximate population coverage of 33% participated. RESULTS: A total 7,140 pneumoniae corresponding to the same number of patients were studied...
July 25, 2016: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/27451855/rapid-and-accurate-detection-of-carbapenemase-genes-in-enterobacteriaceae-with-the-cepheid-xpert-carba-r-assay
#19
Marion Smith, Bram Diederen, Jelle Scharringa, Maurine Leversteijn-van Hall, Ad C Fluit, James Cohen Stuart
Rapid and accurate detection of carbapenemase-producing Enterobacteriaceae is pivotal for adequate antibiotic therapy and infection control. The Cepheid Xpert Carba-R assay detects and identifies the most prevalent carbapenemases (KPC, VIM, IMP, NDM and OXA-48), using automated real-time PCR. The test performance of the Xpert Carba-R was evaluated with 129 well-characterized non-repeat Enterobacteriaceae isolates, suspected for carbapenemase production, i.e. with meropenem MICs >0.25 mg l-1. The isolate collection contained 100 carbapenemase-producing isolates (36 KPC-2 or KPC-3, 20 VIM-1, 4 KPC-2 plus VIM-1, 5 NDM-1, 2 IMP-1, 1 IMP-28, 1 IMP-1 plus VIM-1 and 31 OXA-48 like) and 29 negative control isolates producing extended-spectrum β-lactamase and/or AmpC β-lactamases...
September 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27436389/molecular-typing-and-genetic-environment-of-the-blakpc-gene-in-chilean-isolates-of-klebsiella-pneumoniae
#20
Carla Barría-Loaiza, Andrea Pincheira, Mario Quezada, Alejandra Vera, Pedro Valenzuela, Mariana Domínguez, Celia A Lima, Ingrid Araya, Pamela Araya, Soledad Prat, Carolina Aguayo, Jorge Fernández, Juan Carlos Hormazábal, Helia Bello-Toledo, Gerardo González-Rocha
The aim of this work was to determine the genetic environment and transferability of blaKPC as well as the pulsotypes of KPC-producing Klebsiella pneumoniae strains isolated from clinical samples in Chilean hospitals. Seventeen strains, principally isolated in Santiago (the capital of Chile) during the years 2012 and 2013, were included. The genetic environment of blaKPC was elucidated by PCR mapping and sequencing. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)...
March 2016: Journal of Global Antimicrobial Resistance
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