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https://www.readbyqxmd.com/read/29140102/comparative-genomics-of-type-1-incc-plasmids-from-china
#1
Lizhi Ma, Zhe Yin, Defu Zhang, Zhe Zhan, Qian Wang, Xiongbo Duan, Huixia Gao, Quanhui Liang, Yuzong Zhao, Jiao Feng, Yachao Zhao, Yigang Tong, Erhei Dai, Dongsheng Zhou
AIM: This study dealt with genomic characterization of type 1 IncC resistance plasmids, capable of spreading across taxonomic borders, from China. MATERIALS & METHODS: p112298-tetA was sequenced and compared with type 1 IncC reference plasmid pR148 and two available sequenced type 1 IncC plasmids pHS36-NDM and pVAS3-1 from China. RESULTS: These plasmids contained one or more exogenous resistance islands, which included the ARI-A islands, the ARI-B islands, the ISEcp1-blaCMY units and the bla KPC-2 region and were inserted at various sites in the IncC backbone and thus represented three distinct lineages...
November 15, 2017: Future Microbiology
https://www.readbyqxmd.com/read/29126236/identification-and-genomic-characterization-of-a-kpc-2-ndm-1-and-ndm-5-producing-klebsiella-michiganensis-isolate
#2
Beiwen Zheng, Hao Xu, Xiao Yu, Tao Lv, Xiawei Jiang, Hong Cheng, Jing Zhang, Yunbo Chen, Chen Huang, Yonghong Xiao
No abstract text is available yet for this article.
November 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29122729/draft-genome-sequence-of-enterobacter-cloacae-st128-a-clinical-strain-coproducing-kpc-2-and-ndm-1-carbapenemases
#3
Xi Li, Yongze Zhu, Mengyuan Shen, Jing Du, Lei Zhang, Dairong Wang
OBJECTIVES: Enterobacter cloacae is one of the major pathogens responsible for a variety of human infections. Here, we report the draft genome sequence of a multidrug-resistant E. cloacae strain HBY that was isolated from a female in China. METHODS: Whole-genomic DNA of E. cloacae strain HBY was extracted and was sequenced using an Illumina-Hiseq™ 2000 platform. The generated sequence reads were assembled using CLC Genomics Workbench. The draft genome was annotated using Rapid Annotation using Subsystem Technology and the presence of antimicrobial resistance genes was identified...
November 6, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29110143/pharmacological-aspects-and-spectrum-of-action-of-ceftazidime-avibactam-a-systematic-review
#4
REVIEW
Felipe Francisco Tuon, Jaime L Rocha, Marcelo R Formigoni-Pinto
PURPOSE: Ceftazidime-avibactam is an antimicrobial association active against several Enterobacteriaceae species, including those resistant to carbapenem. Considering the importance of this drug in the current panorama of multidrug-resistant bacteria, we performed a systematic review about ceftazidime-avibactam with emphasis on clinical and pharmacological published data. METHODS: A systematic search of the medical literature was performed. The databases searched included MEDLINE, EMBASE and Web of Science (until September 2017)...
November 7, 2017: Infection
https://www.readbyqxmd.com/read/29101083/draft-genome-sequence-of-a-tigecycline-resistant-kpc-2-producing-klebsiella-pneumoniae-st15-clinical-isolate-from-china
#5
Juan Xu, Meng Gao, Yan Hong, Fang He
OBJECTIVES: Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections have emerged worldwide as a serious challenge to public health. Tigecycline is regarded as one of the last-resort antimicrobials for CRKP infections. Here we report the draft genome sequence of a clinical tigecycline-resistant KPC-2-producing K. pneumoniae isolate (TRKP1). METHODS: Whole-genome sequencing of strain TRKP1 was performed using an Illumina Hiseq™ X Ten system. The generated reads were assembled using CLC Genomics Workbench...
October 31, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29092035/complete-nucleotide-sequences-of-two-kpc-2-encoding-plasmids-from-the-same-citrobacter-freundii-isolate
#6
Beiwen Zheng, Chen Huang, Hao Xu, Xiao Yu, Jing Zhang, Xin Wang, Xiawei Jiang, Yonghong Xiao, Lanjuan Li
No abstract text is available yet for this article.
October 30, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29089425/klebsiella-pneumoniae-carbapenemase-2-kpc-2-substitutions-at-ambler-position-asp179-and-resistance-to-ceftazidime-avibactam-unique-antibiotic-resistant-phenotypes-emerge-from-%C3%AE-lactamase-protein-engineering
#7
Melissa D Barnes, Marisa L Winkler, Magdalena A Taracila, Malcolm G Page, Eric Desarbre, Barry N Kreiswirth, Ryan K Shields, Minh-Hong Nguyen, Cornelius Clancy, Brad Spellberg, Krisztina M Papp-Wallace, Robert A Bonomo
The emergence of Klebsiella pneumoniae carbapenemases (KPCs), β-lactamases that inactivate "last-line" antibiotics such as imipenem, represents a major challenge to contemporary antibiotic therapies. The combination of ceftazidime (CAZ) and avibactam (AVI), a potent β-lactamase inhibitor, represents an attempt to overcome this formidable threat and to restore the efficacy of the antibiotic against Gram-negative bacteria bearing KPCs. CAZ-AVI-resistant clinical strains expressing KPC variants with substitutions in the Ω-loop are emerging...
October 31, 2017: MBio
https://www.readbyqxmd.com/read/29071814/extensively-drug-resistant-escherichia-coli-sequence-type-1642-carrying-an-incx3-plasmid-containing-the-blakpc-2-gene-associated-with-transposon-tn4401a
#8
Seri Jeong, Jung Ok Kim, Eun Jeong Yoon, Il Kwon Bae, Woonhyoung Lee, Hyukmin Lee, Yongjung Park, Kyungwon Lee, Seok Hoon Jeong
BACKGROUND: Extensively drug-resistant (XDR) Enterobacteriaceae carrying the bla(KPC) gene have emerged as a major global therapeutic concern. The purpose of this study was to analyze the complete sequences of plasmids from KPC-2 carbapenemase-producing XDR Escherichia coli sequence type (ST) 1642 isolates. METHODS: We performed antimicrobial susceptibility testing, PCR, multilocus sequence typing (MLST), and whole-genome sequencing to characterize the plasmid-mediated KPC-2-producing E...
January 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29036991/-molecular-epidemiology-of-carbapenem-resistant-klebsiella-pneumoniae-in-a-hospital-in-beijing
#9
D K Chen, H J Zhou
Objective: To reveal the molecular epidemiological characteristics of Carbapenem-Resistant Klebsiella pneumoniae (CRKP) isolated from a three level teaching hospital in Beijing. Methods: Pulsed field gel electrophoresis (PFGE) was carried out to subtyping 375 CRKP isolated in that hospital between May 2010 and October 2015. Fifteen strains were chose based on the PFGE patterns to be analyzed by multi-locus sequence typing (MLST) and detection of carbapenem-resistance genes. One strain (A1502) was selected for whole genome sequencing and analyzing...
October 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29029114/carbapenem-resistant-citrobacter-spp-isolated-in-spain-from-2013-to-2015-produced-a-variety-of-carbapenemases-including-vim-1-oxa-48-kpc-2-ndm-1-and-vim-2
#10
David M Arana, Adriana Ortega, Eva González-Barberá, Noelia Lara, Verónica Bautista, Dolores Gómez-Ruíz, David Sáez, Sara Fernández-Romero, Belén Aracil, María Pérez-Vázquez, José Campos, Jesús Oteo
Objectives: There is little information about carbapenemase-producing (CP) Citrobacter spp. We studied the molecular epidemiology and microbiological features of CP Citrobacter spp. isolates collected in Spain (2013-15). Methods: In total, 119 isolates suspected of being CP by the EUCAST screening cut-off values were analysed. Carbapenemases and ESBLs were characterized using PCR and sequencing. The genetic relationship among Citrobacter freundii isolates was studied by PFGE...
September 27, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29029065/emergence-of-escherichia-coli-co-producing-ndm-1-and-kpc-2-carbapenemases-from-a-retail-vegetable-china
#11
Jing Wang, Xu Yao, Juan Luo, Luchao Lv, Zhenling Zeng, Jian-Hua Liu
No abstract text is available yet for this article.
September 27, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29021086/genomic-characterization-of-an-extensively-drug-resistant-kpc-2-producing-klebsiella-pneumoniae-st855-cc258-only-susceptible-to-ceftazidime-avibactam-isolated-in-brazil
#12
Caio Augusto Martins Aires, Michael J Rybak, Juwon Yim, Polyana Silva Pereira, Cláudio M Rocha-de-Souza, Rodolpho Mattos Albano, Valdelucia Oliveira Cavalcanti, Ana Paula D'Alincourt Carvalho-Assef, Marisa Zenaide Ribeiro Gomes, Marise Dutra Asensi
In this study, we describe the genetic features of an XDR KPC-2-producing Klebsiella pneumoniae isolate by using whole-genome sequencing, its clinical-epidemiological context and susceptibility against antimicrobial combinations. The isolate belongs to clonal complex 258 and harbors several acquired genes and mutations associated with antimicrobial resistance.
August 25, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28971872/kinetics-of-sulbactam-hydrolysis-by-%C3%AE-lactamases-and-their-inhibition
#13
Adam B Shapiro
Sulbactam is one of 4 β-lactamase inhibitors in current clinical use to counteract drug resistance caused by degradation of β-lactam antibiotics by these bacterial enzymes. As a β-lactam itself, sulbactam is susceptible to degradation by β-lactamases. We investigated the Michaelis-Menten kinetics of sulbactam hydrolysis by 14 β-lactamases representing clinically widespread groups within all 4 Ambler classes: CTX-M-15, KPC-2, SHV-5, and TEM-1 for class A; IMP-1, NDM-1, and VIM-1 for class B; Acinetobacter baumannii ADC-7, Pseudomonas aeruginosa AmpC and Enterobacter cloacae P99 for class C; and OXA-10, OXA-23, OXA-24, and OXA-48 for class D...
October 2, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28947474/pharmacokinetics-pharmacodynamics-of-cb-618-in-combination-with-cefepime-ceftazidime-ceftolozane-and-meropenem-the-pharmacological-basis-for-a-stand-alone-%C3%AE-lactamase-inhibitor
#14
Paul G Ambrose, Brian D VanScoy, Michael Trang, Jennifer McCauley-Miller, Haley Conde, Sujata M Bhavnani, Dylan C Alexander, Lawrence V Friedrich
A major challenge in treating patients is the selection of the "right" antibiotic regimen. Given that the optimal β-lactam-β-lactamase inhibitor pair is dependent upon the spectrum of β-lactamase enzymes produced and the frequency of resistance to the β-lactamase inhibitor, it might be useful if a stand-alone were available for the clinician to pair with the "right" β-lactam rather than only in a fixed combination. In this communication, we describe a one-compartment in vitro infection model studies conducted to identify the magnitude of the pharmacokinetic-pharmacodynamic (PK-PD) index for a β-lactamase inhibitor, CB-618, that would restore the activity of four β-lactam partner agents (cefepime, ceftazidime, ceftolozane, and meropenem) with varying dose (1 or 2 g) and dosing intervals (8 or 12 h)...
September 25, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28935481/stalking-a-lethal-superbug-by-whole-genome-sequencing-and-phylogenetics-influence-on-unraveling-a-major-hospital-outbreak-of-carbapenem-resistant-klebsiella-pneumoniae
#15
Thorsten Kaiser, Knut Finstermeier, Madlen Häntzsch, Sarah Faucheux, Martin Kaase, Tim Eckmanns, Sven Bercker, Udo X Kaisers, Norman Lippmann, Arne C Rodloff, Joachim Thiery, Christoph Lübbert
BACKGROUND: From July 2010-April 2013, Leipzig University Hospital experienced the largest outbreak of a Klebsiella pneumoniae carbapenemase 2 (KPC-2)-producing Klebsiella pneumoniae (KPC-2-Kp) strain observed in Germany to date. After termination of the outbreak, we aimed to reconstruct transmission pathways by phylogenetics based on whole-genome sequencing (WGS). METHODS: One hundred seventeen KPC-2-Kp isolates from 89 outbreak patients, 5 environmental KPC-2-Kp isolates, and 24 K pneumoniae strains not linked to the outbreak underwent WGS...
September 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28887199/temporal-evolution-of-polymyxin-b-resistant-klebsiella-pneumoniae-clones-recovered-from-blood-cultures-in-a-teaching-hospital-during-a-7-year-period
#16
Graziela Braun, Rodrigo Cayô, Adriana Pereira Matos, Juliane de Mello Fonseca, Ana Cristina Gales
The polymyxins have become one of the last resorts to treat serious infections caused by KPC-2-producing Klebsiella pneumoniae worldwide. However, the increase of polymyxin consumption has favored the emergence of resistance to these compounds. In this study we observed an increase in the polymyxin B resistance rates from 0 to 30.6% among 224 K. pneumoniae isolates recovered from blood cultures between 2009 and 2015. Only gentamicin, tigecycline and fosfomycin remained active against the polymyxin B-resistant K...
September 5, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28874377/high-level-resistance-to-colistin-mediated-by-various-mutations-in-the-crrb-gene-among-carbapenemase-producing-klebsiella-pneumoniae
#17
Aurélie Jayol, Patrice Nordmann, Adrian Brink, Maria-Virginia Villegas, Véronique Dubois, Laurent Poirel
Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype...
November 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28844719/evaluation-of-a-rapid-immunochromatographic-test-for-detection-of-distinct-variants-of-klebsiella-pneumoniae-carbapenemase-kpc-in-enterobacteriaceae
#18
Ana C Ramos, Ana C Gales, Jussimara Monteiro, Suzane Silbert, Thomás Chagas-Neto, Antonia M O Machado, Cecilia G Carvalhaes
The rapid detection of KPC-producing Enterobacteriaceae by microbiology laboratories has been required for infectious control programs. Herein we evaluated the performance of a novel immunochromatographic test for detecting KPC-2-, KPC-3-, KPC-4-, KPC-6-, KPC-7-, KPC-8-, and KPC-11-producing isolates and the influence of different growth media on the test performance.
November 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28840351/activity-of-temocillin-and-15-other-agents-including-fosfomycin-and-colistin-against-enterobacteriaceae-in-hong-kong
#19
Margaret Ip, Christopher K Lai, Kitty S C Fung, K-Tak Wong, Chendi Zhu, Sebastien Van de Velde, Dominic N Tsang, Peter Hawkey
Limited data are available on temocillin susceptibilities in Enterobacteriaceae from Asian countries where antimicrobial resistance is prevalent. The in vitro activities of temocillin and 15 commonly used antimicrobials against 613 non-duplicate blood (n = 310) and urine (with clinically significant bacteriuria; n = 303) isolates of Enterobacteriaceae from patients who attended 3 out of 7 clusters of public hospitals of the Hospital Authority, Hong Kong, during 2015/2016 were tested. Minimum inhibitory concentrations (MICs) were determined by Clinical and Laboratory Standards Institute (CLSI) microbroth dilution (agar dilution with fosfomycin)...
August 25, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28835096/reversibility-of-covalent-broad-spectrum-serine-%C3%AE-lactamase-inhibition-by-the-diazabicyclooctenone-etx2514
#20
Adam B Shapiro, Ning Gao, Haris Jahić, Nicole M Carter, April Chen, Alita A Miller
ETX2514 is a non-β-lactam serine β-lactamase inhibitor in clinical development that has greater potency and broader spectrum of β-lactamase inhibition than the related diazabicyclooctanone avibactam. Despite opening of its cyclic urea ring upon acylation, avibactam can recyclize and dissociate intact from certain β-lactamases. We investigated reversibility of ETX2514 acylation of 10 serine β-lactamases representing Ambler classes A, C, and D. Dissociation rate constants varied widely between enzymes and were lowest for class D...
August 28, 2017: ACS Infectious Diseases
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