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https://www.readbyqxmd.com/read/28219824/characterisation-of-the-first-kpc-2-producing-klebsiella-pneumoniae-st340-from-peru
#1
Gertrudis Horna, Jorge Velasquez, Nathaly Fernández, Jesus Tamariz, Joaquim Ruiz
OBJECTIVES: The aim of this study was to characterise a KPC-carrying Klebsiella pneumoniae isolate from a Peruvian hospital setting. METHODS: The identity of the isolate was confirmed by amplification and sequencing of the 16S rRNA gene, and the antibiotic resistance profile was determined by disk diffusion and automated METHODS: The sequence type (ST) and phylogenetic group were established by PCR. The presence of different β-lactamase genes was determined, including blaMBL, blaKPC, blaCTX-M, blaSHV, blaOXA-1-like, blaOXA-2-like, blaOXA-5-like, blaOXA-48-like and blaTEM and up to six different plasmid-encoded AmpC genes as well as class 1 integrons...
February 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28210888/double-carbapenem-combination-as-salvage-therapy-for-untreatable-infections-by-kpc-2-producing-klebsiella-pneumoniae
#2
M Souli, I Karaiskos, A Masgala, L Galani, E Barmpouti, H Giamarellou
We report our experience using the double-carbapenem combination as salvage therapy for patients with untreatable infections caused by KPC-2- producing Klebsiella pneumoniae. A total of 27 patients in two institutions in Athens, Greece suffering from complicated urinary tract infections (16) with or without secondary bacteraemia (four and 12 respectively), primary (six) or catheter-related bloodstream infections (two), HAP or VAP (two) and external ventricular drainage infection (one) were treated exclusively with ertapenem and high-dose prolonged infusion meropenem because in-vitro active antimicrobials were unavailable (19) or failed (four) or were contraindicated (six)...
February 16, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28185686/outbreak-of-kpc-2-producing-enterobacteriaceae-caused-by-clonal-dissemination-of-klebsiella-pneumoniae-st307-carrying-an-incx3-type-plasmid-harboring-a-truncated-tn4401a
#3
Jung Ok Kim, Sae Am Song, Eun-Jeong Yoon, Jeong Hwan Shin, Hyukmin Lee, Seok Hoon Jeong, Kyungwon Lee
Over a 5-month period between the end of June and the beginning of November in 2015, a KPC-producing Enterobacteriaceae outbreak occurred in a general hospital in Busan, South Korea, being associated with a total of 50 clinical isolates from 47 patients. Multilocus sequence typing and pulsed-field gel electrophoresis were carried out for strain typing and whole-genome sequencing was performed to characterize the plasmids. A clonal spread of K. pneumoniae sequence type 307 (ST307) carrying a self-transferable IncX3-type plasmid harboring blaKPC-2 was responsible for the outbreak...
December 27, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28167551/genomic-characterization-of-two-kpc-producing-klebsiella-isolates-collected-in-1997-from-new-york-city
#4
Brandon Eilertson, Liang Chen, Kalyan D Chavda, Barry N Kreiswirth
Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have now become a global public health threat. However, the origin of this pandemic and characterization of pre-2003 blaKPC-harboring plasmids remain unknown. Here we used next generation sequencing to characterize two KPC-2-producing K. pneumoniae and K. michiganensis isolates, collected from a New York City hospital in 1997. Although identified in two different Klebsiella species, the blaKPC-2 gene is harbored by Tn4401b transposon on two highly similar IncN plasmids...
February 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28167549/polymyxin-b-based-triple-combinations-wage-war-against-kpc-2-producing-klebsiella-pneumoniae-new-dosing-strategies-for-old-allies
#5
Zackery P Bulman, Michael J Satlin, Liang Chen, Barry N Kreiswirth, Beom Soo Shin, Thomas J Walsh, Patricia N Holden, Alan Forrest, Roger L Nation, Jian Li, Brian T Tsuji
Pharmacodynamics of a polymyxin B, meropenem and rifampin triple combination were examined against Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae (KPC-Kp) ST258. In time-kill experiments against three KPC-Kp isolates, triple combination generated 8.14, 8.19 and 8.29 log10CFU/mL reductions within 24h. In the hollow fiber infection model, the triple combination caused maximal killing of 5.16 log10CFU/mL at 78h and time required for regrowth was more than doubled versus 2-drug combinations...
February 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28167547/bacteremia-due-to-carbapenem-resistant-enterobacteriaceae-cre-a-multicenter-clinical-and-molecular-epidemiologic-analysis-in-the-nation-s-epicenter-for-cre
#6
Michael J Satlin, Liang Chen, Gopi Patel, Angela Gomez-Simmonds, Gregory Weston, Angela C Kim, Susan K Seo, Marnie E Rosenthal, Steven J Sperber, Stephen G Jenkins, Camille L Hamula, Anne-Catrin Uhlemann, Michael H Levi, Bettina C Fries, Yi-Wei Tang, Stefan Juretschko, Albert D Rojtman, Tao Hong, Barun Mathema, Michael R Jacobs, Thomas J Walsh, Robert A Bonomo, Barry N Kreiswirth
Although the New York/New Jersey (NY/NJ) area is an epicenter for carbapenem-resistant Enterobacteriaceae (CRE), there are few multicenter studies of CRE from this region. We characterized patients with CRE bacteremia in 2013 at eight NY/NJ medical centers and determined the prevalence of carbapenem resistance among Enterobacteriaceae bloodstream isolates and CRE resistance mechanisms, genetic backgrounds, capsular types (cps), and antimicrobial susceptibilities. Of 121 patients with CRE bacteremia, 50% had cancer or transplantation...
February 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28132768/bacteremia-and-other-body-site-infection-caused-by-hypervirulent-and-classic-klebsiella-pneumoniae
#7
Hua Wu, Dongdong Li, Haijian Zhou, Yunfang Sun, Ling Guo, Dingxia Shen
OBJECTIVES: To investigate bacteremia and other body site infection caused by hypervirulent Klebsiella pneumoniae (hvKP), a recently recognized pathogen of invasive infection, and classic Klebsiella pneumoniae (cKP), a very common organism associated with many kinds of nosocomial infection. METHODS: Clinical information obtained from patients with both bacteremia and other body site infections caused by hvKP and/or cKP was retrospectively reviewed. Homo-hvKP (or homo-cKP) was defined as homologous hvKP (or cKP) strains from different body sites in each individual patient according to string test, virulence gene amplification and PFGE pattern...
March 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28069651/inhibition-by-avibactam-and-clavulanate-of-the-%C3%AE-lactamases-kpc-2-and-ctx-m-15-harboring-the-substitution-n-132-g-in-the-conserved-sdn-motif
#8
Clément Ourghanlian, Daria Soroka, Michel Arthur
The substitution N(132)G in the SDN motif of class A β-lactamases from rapidly growing mycobacteria was previously shown to impair their inhibition by avibactam but to improve the stability of acyl-enzymes formed with clavulanate. The same substitution was introduced in KPC-2 and CTX-M-15 to assess its impact on β-lactamases from Enterobacteriaceae and evaluate whether it may lead to resistance to the ceftazidime-avibactam combination. Kinetic parameters for the inhibition of the β-lactamases by avibactam and clavulanate were determined by spectrophotometry using nitrocefin as the substrate...
March 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28065546/discharge-of-kpc-2-genes-from-the-wwtps-contributed-to-their-enriched-abundance-in-the-receiving-river
#9
Fengxia Yang, Liang Huang, Linyun Li, Yang Yang, Daqing Mao, Yi Luo
At present, very little is known about the persistence and spread pathway of KPC-2 genes in the environment. Our previous study reported the prevalence and persistence of KPC-2 genes in wastewater treatment plants (WWTPs). In the present work, we investigated the occurrence and fate of KPC-2 genes in a WWTP discharge-receiving river and studied the effect of WWTP discharges on the prevalence of KPC-2 genes and host bacteria in the receiving river. It is observed that a considerable level of KPC-2 genes occurred in the receiving river, and a significant increase of blaKPC-2 abundance in the downstream following WWTP discharge was observed compared to the upstream...
January 5, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28049378/isolation-of-escherichia-coli-st131-producing-kpc-2-in-bulgaria
#10
Rumyana Markovska, Temenuga Stoeva, Lyudmila Boyanova, Petya Stankova, Daniela Pencheva, Radka Kaneva, Vanyo Mitev, Ivan Mitov
No abstract text is available yet for this article.
January 3, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/27927163/phenotypic-and-molecular-detection-of-the-bla-kpc-gene-in-clinical-isolates-from-inpatients-at-hospitals-in-s%C3%A3-o-luis-ma-brazil
#11
Patricia Cristina Saldanha Ribeiro, Andrea Souza Monteiro, Sirlei Garcia Marques, Sílvio Gomes Monteiro, Valério Monteiro-Neto, Martina Márcia Melo Coqueiro, Ana Cláudia Garcia Marques, Rosimary de Jesus Gomes Turri, Simone Gonçalves Santos, Maria Rosa Quaresma Bomfim
BACKGROUND: Bacteria that produce Klebsiella pneumoniae carbapenemases (KPCs) are resistant to broad-spectrum β-lactam antibiotics. The objective of this study was to phenotypically and genotypically characterize the antibiotic susceptibility to carbapenems of 297 isolates recovered from clinical samples obtained from inpatients at 16 hospitals in São Luis (Maranhão, Brazil). METHODS: The study was conducted using phenotypic tests and molecular methods, including polymerase chain reaction (PCR), sequencing and enterobacterial repetitive intergenic consensus (ERIC)-PCR...
December 7, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27890667/investigation-of-carbapenem-resistance-and-the-first-identification-of-klebsiella-pneumoniae-carbapenemase-kpc-enzyme-among-escherichia-coli-isolates-in-turkey-a-prospective-study
#12
Mert Ahmet Kuskucu, Asiye Karakullukcu, Mailihaba Ailiken, Barıs Otlu, Bilgul Mete, Gokhan Aygun
BACKGROUND: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coli isolates from clinical samples in Turkey. METHODS: The prospective study included a total of 4.052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed-field gel electrophoresis (PFGE), respectively...
November 2016: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/27825605/antimicrobial-resistance-in-enterobacteriaceae-in-brazil-focus-on-%C3%AE-lactams-and-polymyxins
#13
REVIEW
Jorge Luiz Mello Sampaio, Ana Cristina Gales
During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96...
December 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27812159/enterobacteriaceae-isolated-from-the-river-danube-antibiotic-resistances-with-a-focus-on-the-presence-of-esbl-and-carbapenemases
#14
Clemens Kittinger, Michaela Lipp, Bettina Folli, Alexander Kirschner, Rita Baumert, Herbert Galler, Andrea J Grisold, Josefa Luxner, Melanie Weissenbacher, Andreas H Farnleitner, Gernot Zarfel
In a clinical setting it seems to be normal these days that a relevant proportion or even the majority of different bacterial species has already one or more acquired antibiotic resistances. Unfortunately, the overuse of antibiotics for livestock breeding and medicine has also altered the wild-type resistance profiles of many bacterial species in different environmental settings. As a matter of fact, getting in contact with resistant bacteria is no longer restricted to hospitals. Beside food and food production, the aquatic environment might also play an important role as reservoir and carrier...
2016: PloS One
https://www.readbyqxmd.com/read/27811089/draft-genome-sequence-of-a-hospital-associated-clone-of-klebsiella-pneumoniae-st340-cc258-coproducing-rmtg-and-kpc-2-isolated-from-a-pediatric-patient
#15
Louise Cerdeira, Miriam R Fernandes, Gabriela R Francisco, Maria Fernanda C Bueno, Susan Ienne, Tiago A Souza, Doroti de Oliveira Garcia, Nilton Lincopan
We report here the draft genome sequence of a Klebsiella pneumoniae strain 1194/11, belonging to the hospital-associated sequence type 340 (ST340; clonal complex CC258), isolated from a catheter tip culture from a pediatric patient. The multidrug-resistant strain coproduced the 16S rRNA methyltransferase rRNA RmtG and β-lactamases KPC-2 and CTX-M-15.
November 3, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27770796/a-cluster-of-kpc-2-and-vim-2-producing-klebsiella-pneumoniae-st833-isolates-from-the-pediatric-service-of-a-venezuelan-hospital
#16
Aura Falco, Yusibeska Ramos, Esther Franco, Alegría Guzmán, Howard Takiff
BACKGROUND: Klebsiella pneumoniae is a bacterial pathogen that has developed resistance to multiple antibiotics and is a major cause of nosocomial infections worldwide. Carbapenemase-producing Klebsiella pneumoniae have been isolated in many hospitals in Venezuela, but they have not been well-studied. The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae isolates from the pediatric service of a hospital located in Anzoategui State, in the eastern part of Venezuela...
October 22, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27756125/engineering-specificity-from-broad-to-narrow-design-of-a-%C3%AE-lactamase-inhibitory-protein-blip-variant-that-exclusively-binds-and-detects-kpc-%C3%AE-lactamase
#17
Dar-Chone Chow, Kacie Rice, Wanzhi Huang, Robert L Atmar, Timothy Palzkill
The β-lactamase inhibitory protein (BLIP) binds and inhibits a wide range of class A β-lactamases including the TEM-1 β-lactamase (Ki = 0.5 nM), which is widely present in Gram-negative bacteria, and the KPC-2 β-lactamase (Ki = 1.2 nM), which hydrolyzes virtually all clinically useful β-lactam antibiotics. The extent to which the specificity of a protein that binds a broad range of targets can be modified to display narrow specificity was explored in this study by engineering BLIP to bind selectively to KPC-2 β-lactamase...
December 9, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27754776/first-description-of-kpc-2-producing-escherichia-coli-and-st15-oxa-48-positive-klebsiella-pneumoniae-in-tunisia
#18
Farah Ben Tanfous, Carla Andrea Alonso, Wafa Achour, Laura Ruiz-Ripa, Carmen Torres, Assia Ben Hassen
The aim of this study was to investigate the molecular features among Klebsiella pneumoniae and Escherichia coli strains showing a resistant/intermediate-resistant phenotype to ertapenem (R/IR-ERT), implicated in colonization/infection in patients of the Hematology and Graft Units of the National Bone Marrow Transplant Center of Tunisia (3-year period, 2011-2014). The major carbapenemase, extended-spectrum beta-lactamase, and plasmidic AmpC beta-lactamase genes were analyzed and characterized by PCR and sequencing...
October 18, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27733128/nosocomial-outbreak-of-kpc-2-and-ndm-1-producing-klebsiella-pneumoniae-in-a-neonatal-ward-a-retrospective-study
#19
Jing Yu, Kun Tan, Zhihui Rong, Yue Wang, Zhongju Chen, Xuhui Zhu, Li Wu, Li Tan, Wei Xiong, Ziyong Sun, Ling Chen
BACKGROUND: The spread of resistance to carbapenems among Enterobacteriaceae has become a major public health problem in recent years. In this study, we describe an outbreak of Klebsiella pneumoniae in the neonatal ward. First, we aimed to study the drug resistance, genetic relatedness, and transmission mechanism of carbapenem-resistant K. pneumoniae; second, we implemented infection control measures to contain the outbreak. METHODS: We investigated 27 non-repetitive strains isolated from neonates and five strains cultured from around the neonatal ward...
October 12, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27691984/increase-in-prevalence-of-kpc-2-producing-klebsiella-pneumoniae-recovered-from-respiratory-secretions-of-intensive-care-patients-getting-a-free-ride-on-a-menacing-colistin-resistance
#20
Leandro Reus Rodrigues Perez
No abstract text is available yet for this article.
December 2016: Infection Control and Hospital Epidemiology
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