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Falin He, Wei Wang, Kun Zhong, Shuai Yuan, Zhiguo Wang
BACKGROUND: This national survey was initiated to investigate the current status of quality control practice of prenatal screening by statistical analysis of the previous half year data of prenatal screening in 2015. METHODS: Data were sent to all Chinese prenatal screening centers via the National Quality Assessment Scheme. This covered the software used, the risk cutoffs, monthly sample throughput, monthly median MoM of AFP, HCG, β-HCG, free β-HCG and uE3, monthly screening positive rates for trisomy 21, trisomy 18, and Open Neural Tube Defect (ONTD)...
January 1, 2017: Clinical Laboratory
Feng Ren, Y U Hu, Huan Zhou, Wen-Ying Zhu, L I Jia, Jing-Jing Xu, Jing Xue
The aim of the present study was to evaluate the serum levels of α-fetoprotein (AFP), free-β-human chorionic gonadotropin (free-β-hCG) and unconjugated estriol (uE3) in second trimester maternal serum and comparative analysis of serum markers levels in normal twin and singleton pregnancies, and to evaluate the feasible methods for the screening performances of triple markers in twin pregnancy. The levels of maternal serum markers were measured by magnetic microparticle chemiluminescence immunoassay in the second trimester...
April 2016: Biomedical Reports
A L David, E Jauniaux
INTRODUCTION: To study the relationship between 2-dimensional placental ultrasound measurements and maternal serum (MS) levels of biomarkers of placentation and in pregnancies presenting with an isolated abnormally high or low birthweight at term, without evidence of placental insufficiency. METHOD: We performed a population based cohort study of 306 pregnancies delivered at term including 30 presenting with large-for-gestational age (LGA, birthweight > 90th centile) and 17 small-for-gestational age (SGA; birthweight < 10th centile)...
April 2016: Placenta
Lei Huai, Jianhang Leng, Shenglin Ma, Fang Huang, Junya Shen, Yu Ding
This study aimed to investigate the serum concentration of alpha-fetoprotein (AFP)-L3 in midterm pregnancies and its potential application in prenatal trisomy screening. The serum samples from 27 women with trisomy 21 fetuses and 800 women with normal fetuses were examined to measure the concentrations of AFP, AFP-L3, human chorionic gonadotropin (hCG), unconjugated estriol (uE3), and inhibin-A. The screening results of various tests consisting of these markers were analyzed. In normal pregnancies within 15-20 weeks of gestation, the medians of serum AFP-L3 were 4...
November 2016: American Journal of Therapeutics
Georgina Wilson, Päivi Liitti, Tuukka Pölönen, Mikko Sairanen, Kevin Spencer
BACKGROUND: The objective of the study was to compare a new AutoDELFIA® Inhibin A kit (B064-102) with the Access Inhibin A kit (A36097) using clinical specimens and to evaluate the AutoDELFIA® Inhibin A assay performance in screening for Down syndrome in the second trimester of pregnancy. METHODS: Using clinical samples, we performed a method comparison between new and existing inhibin A kits and assessed AutoDELFIA® Inhibin A kit precision performance. Normal median values for the second trimester of pregnancy were also determined...
September 1, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
S Kate Alldred, Yemisi Takwoingi, Boliang Guo, Mary Pennant, Jonathan J Deeks, James P Neilson, Zarko Alfirevic
BACKGROUND: Down's syndrome occurs when a person has three, rather than two copies of chromosome 21; or the specific area of chromosome 21 implicated in causing Down's syndrome. It is the commonest congenital cause of mental disability and also leads to numerous metabolic and structural problems. It can be life-threatening, or lead to considerable ill health, although some individuals have only mild problems and can lead relatively normal lives. Having a baby with Down's syndrome is likely to have a significant impact on family life...
November 30, 2015: Cochrane Database of Systematic Reviews
Gayle C Windham, Kristen Lyall, Meredith Anderson, Martin Kharrazi
We examined prenatal screening markers and offspring autism spectrum disorder (ASD) using California statewide data on singleton births in 1996 and 2002. Second trimester levels of unconjugated estriol (uE3), human chorionic gonadotropin (hCG), and maternal serum alpha-fetoprotein (MSAFP) were compared between mothers of children with ASD (n = 2586) and of non-cases (n = 600,103). Adjusted odds ratios (AOR) were calculated by logistic regression. Lower uE3 (AOR for < 10th percentile vs. 25th-74th percentiles = 1...
February 2016: Journal of Autism and Developmental Disorders
Fang Zhao, Da Chai, Jusheng Lu, Jiachao Yu, Songqin Liu
Novel chemiluminescent (CL) imaging microtiter plates with high-throughput, low-cost, and simple operation for detection of four biomarkers related to Down's syndrome screening were developed and evaluated. To enhance the sensitivity of CL immunosensing, soybean peroxidase (SBP) was used instead of horseradish peroxide (HRP) as a label enzyme. The microtiter plates were fabricated by simultaneously immobilizing four capture monoclonal antibodies, anti-inhibin-A, anti-unconjugated oestriol (anti-uE3), anti-alpha-fetoprotein (anti-AFP), and beta anti-HCG (anti-β-HCG), on nitrocellulose (NC) membrane to form immunosensing microtiter wells...
August 2015: Analytical and Bioanalytical Chemistry
Chao-yan Yue, Bin Zhang, Chun-mei Ying
OBJECTIVES: IL-35 is a novel inhibitory cytokine. In this study, we investigate the serum levels of inhibitory cytokines IL-35, IL-10 and TGF-β in both normal pregnancies and non-pregnant females, and whether IL-35 is associated with the pathogenesis of recurrent spontaneous abortion. We also try to elucidate the relationships of IL-35 with estrogen and alpha-fetoprotein (AFP). METHODS: The levels of IL-35, IL-10, TGF-β, estradiol (E2), unconjugated estriol (uE3) and AFP were analyzed in 120 normal pregnancies, 40 women suffering recurrent spontaneous abortion, 40 postpartum healthy women and 40 non-pregnant women by enzyme-linked immunosorbent assay (ELISA)...
2015: PloS One
Sanaz Mousavi, Sedigheh Hantoushzadeh, Mahdi Sheikh, Mamak Shariat
OBJECTIVE: Assessing the effects of maternal anxiety on the markers and results of quadruple screening and on maternal anxiety after receiving positive results. METHODS: This prospective cohort study evaluated 1,595 pregnant women referred for prenatal visits. Maternal state/trait anxiety levels were measured, then quadruple screening was performed by measuring serum α-fetoprotein (AFP), β-human chorionic gonadotropin, inhibin A, and unconjugated estriol (UE3)...
2015: Fetal Diagnosis and Therapy
Wei Gu, Jing Lin, Yanyan Hou
OBJECTIVE: To discover the value of combined maternal first and second-trimester serum β-hCG, pregnancy associated plasma protein A (PAPP-A), alpha-fetoprotein(AFP)and unconjugated estriol (uE3) in the prediction of preeclampsia. METHODS: A total of 1 805 pregnant women who had antenatal care at International Peace Maternal and Child Health Hospital Affiliated to Shanghai Jiaotong University between April 2012 and June 2013 were selected prospectively by random method...
February 2015: Zhonghua Fu Chan Ke za Zhi
Zhuming Hu, Xiangyin Liu, Linlin Li, Chunshu Jia, Dejun Li, Ruizhi Liu
OBJECTIVE: To investigate the predictive value of abnormal multiples of the median (MoM) of second trimester maternal serum triple screening (STMSTS) markers for adverse pregnancy outcomes. METHODS: 16 000 singleton pregnancies at 15⁺⁰ to 20⁺⁵ weeks' gestation who underwent STMSTS between July 2010 and January 2013 in the First Hospital of Jilin University were recruited. Maternal serum AFP, free β-hCG (F-β-hCG) and unconjugated estriol (uE3) levels were measured using time- resolved fluoroimmunoassay, and then converted to MoM...
October 2014: Zhonghua Fu Chan Ke za Zhi
Nilgün Güdücü, Uzay Görmüş, Ebru İlhan Güner, Omer Güzel, Zehra Neşe Kavak
AIM: Quadruple test is used for Down's syndrome screening in the second trimester of pregnancy. The aim of this study was to investigate differences in quadruple test parameters between pregnancies achieved by assisted reproductive treatments (ART) and spontaneous conception. MATERIALS AND METHODS: We retrospectively compared levels of alfa-fetoprotein (AFP), unconjugated Estriol (uE3), inhibin-A and hCG and also screen positive test results. RESULTS: Levels of all quadruple test parameters were statistically significantly increased in ART pregnancies when compared to spontaneous pregnancies, AFP was 1...
2014: International Journal of Clinical and Experimental Medicine
Tianhua Huang, Karen Boucher, Ritu Aul, Shamim Rashid, Wendy S Meschino
OBJECTIVE: The aim of this study was to assess the concentration of the first and second trimester maternal serum markers in pregnancies with a vanishing twin. METHODS: This is a retrospective case-control study of pregnancies screened for Down syndrome in one Ontario center. Singleton pregnancies with ultrasound evidence of a vanishing twin were identified, and each was matched with five normal singleton controls for ethnicity, maternal age, gestational age, and blood sampling date...
January 2015: Prenatal Diagnosis
L Anderson, M Villiers, L Brown, J Gibson
UNLABELLED: : All women in Scotland are offered first trimester screening combining ultrasound nuchal translucency measurement and free ßhCG and PAPPA-A levels to calculate their risk for Down's syndrome. Second trimester screening may then be offered due to failed NT measurements or later gestation. Although pregnancies affected by Trisomy 13 and 18 are much rarer it is possible to calculate a risk using the same tests. We have discontinued reporting high Trisomy 18/13 risks based on the same biochemical markers following indication that second trimester ultrasound was more sensitive and specific test...
June 2014: Archives of Disease in Childhood. Fetal and Neonatal Edition
N Sarween, I Mills, C Day, G Lipkin, E Knox
INTRODUCTION: In 2001, the UK National Screening Committee advised that all pregnant mothers should be offered a screening test for Down's syndrome (DS). This is performed at first (biomarkers and ultrasound) or second trimester (biochemical screen). There are limited data reporting pregnant women with chronic kidney disease (CKD) have higher false positive screening rates possibly because of reduced clearance of β-hCG (1-3). Our aim was to establish whether standard DS screening is appropriate in pregnant women with renal disease...
June 2014: Archives of Disease in Childhood. Fetal and Neonatal Edition
Sofia Diamantopoulou, Efstratios Papazoglou, Vasileios Margaritis, Afrodite Kakaboura
OBJECTIVES: To compare color and translucency changes after light curing and finishing in various shades of five contemporary resin composites. METHODS: Light and dark (A1 & A4 or equivalents), enamel (e) and dentin (d), shades were selected from the resin composites Enamel Plus HRi (Micerium), Miris 2 (Coltene), IPS Empress Direct (Ivoclar), Filtek Supreme (3M ESPE) and TPH Spectum (Densply). Ten disc-shaped samples (depth = 10 mm, thickness = 1 mm) per material and shade were fabricated...
2014: International Journal of Esthetic Dentistry
N Gruchy, N Bigot, C Jeanne Pasquier, M H Read, S Odent, P Galera, N Leporrier
BACKGROUND: Trisomy 18 and Smith-Lemli-Opitz syndrome are two polymalformative conditions in which a cholesterol defect has been noted. When they occur prenatally, they are associated with a decreased maternal unconjugated estriol (uE(3)) level. Cholesterol plays an essential role in the Sonic Hedgehog pathway, allowing Shh protein maturation leading to its maximal activity. Many malformations in these two syndromes occur in Shh dependent tissues. We thus sought to assess whether a cholesterol defect could affect the Shh pathway and explain some of the observed malformations...
June 2014: Molecular Genetics and Metabolism
Ghada Bourjeily, Kristen Butterfield, Patrizia Curran, Geralyn Lambert-Messerlian
BACKGROUND: Obstructive sleep apnea (OSA) has been associated with adverse fetal outcomes in some studies. Second trimester Down syndrome screening markers reflect fetal and fetoplacental wellbeing. We aimed to compare markers of fetal and feto-placental wellbeing in women with OSA and low risk controls. METHODS: A retrospective case-control study of pregnant women with OSA and available second trimester markers was performed. Controls were screened for sleep disordered breathing (SDB) at the time of delivery using a questionnaire...
February 2015: Journal of Maternal-fetal & Neonatal Medicine
Amanda Roman, Neeraj Desai, David Krantz, Hsiao-Pin Liu, Jonathan Rosner, Nidhi Vohra, Burton Rochelson
OBJECTIVES: Our primary objective was to determine the association of maternal serum analytes in pregnancies complicated by intrauterine growth restriction (IUGR) stratified by umbilical artery (UA) Doppler versus pregnancies with appropriately grown for gestational age (AGA) and its potential use as screening model. METHODS: Retrospective cohort evaluating first and second trimester maternal serum aneuploidy screening markers in women complicated with IUGR [90 with absent or reversed end diastolic velocity (AREDV), 46 with UA systolic/diastolic ratio ≥95th percentile and 215 with normal UA Doppler] versus 2590 women with AGA fetuses (control)...
July 2014: Prenatal Diagnosis
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