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Point of care labs

Cui Hongzhou, Guo Shuping, Wang Wenju, Li Li, Wei Lulu, Deng Linjun, Li Jingmin, Ren Xiaoli, Bai Li
In recent years, molecular biology has proven to be a great asset in our understanding of mechanisms in genodermatoses. However, bench to bedside translation research lags far behind. Advances in lab-on-a-chip technologies enabled programmable, reconfigurable, and scalable manipulation of a variety of laboratory procedures. Sample preparation, microfluidic reactions, and continuous monitoring systems can be integrated on a small chip. These advantages have attracted attention in various fields of clinical application including diagnosis of inherited skin diseases...
September 13, 2016: Journal of Dermatological Science
Andrew Petrosoniak, Marc Auerbach, Ambrose H Wong, Christopher M Hicks
In situ simulation (ISS), a point of care training strategy that occurs within the patient care environment involving actual healthcare team members, provides additional benefits to centre-based simulation. ISS can serve several roles within emergency medicine (EM): improves provider/team performance, identifies and mitigates threats to patient safety and improves systems and infrastructure. The effective use of ISS fosters inter-professional team training and a culture of safety essential for high performance EM teams and resilient systems...
October 17, 2016: Emergency Medicine Australasia: EMA
Samiksha Nayak, Archana Sridhara, Rita Melo, Luciana Richer, Natalie H Chee, Jiyoon Kim, Vincent Linder, David Steinmiller, Samuel K Sia, Maria Gomes-Solecki
Currently, diagnostic testing for Lyme disease is done by determination of the serologic responses to Borrelia burgdorferi antigens, with the exception of the early localized phase of disease where diagnosis must be done clinically. Here, we describe the use of microfluidics technology to develop a multiplexed rapid lab-on-a-chip point of care (POC) assay for the serologic diagnosis of human Lyme disease. Following ELISA screening of 12 candidate antigens, we tested 8 on a microfluidic diagnostic system, called mChip-Ld, using a set of 60 serological samples...
October 11, 2016: Scientific Reports
L Zhang, Q Cai, R S Wiederkehr, M Fauvart, P Fiorini, B Majeed, M Tsukuda, T Matsuno, T Stakenborg
Pharmacogenetics has often been touted as a cornerstone for precision medicine as detailed knowledge of a specific genetic makeup may allow for accurate predictions of a patient's individual drug response. Still, the widespread use of genetic tests is limited as they remain expensive and cumbersome, requiring sophisticated tools and highly trained personnel. In order for pharmacogenetics to reach its full potential, more cost-effective and easily accessible genotyping methods are desired. To meet these challenges, we present a silicon-based integrated microsystem for the detection of multiple single nucleotide polymorphisms (SNPs) directly from human blood...
October 5, 2016: Lab on a Chip
Jason C Harper, Jenna M Andrews, Candice Ben, Andrew C Hunt, Jaclyn K Murton, Bryan D Carson, George D Bachand, Julie A Lovchik, William D Arndt, Melissa R Finley, Thayne L Edwards
Since the introduction of micro total analytical systems (μTASs), significant advances have been made toward development of lab-on-a-chip platforms capable of performing complex biological assays that can revolutionize public health, among other applications. However, use of these platforms in low-resource environments (e.g. developing countries) has yet to be realized as the majority of technologies used to control microfluidic flow rely on off-device hardware with non-negligible size, cost, power requirements and skill/training to operate...
October 18, 2016: Lab on a Chip
Zequn Yan, Min Xue, Qian He, Wei Lu, Zihui Meng, Dan Yan, Lili Qiu, Lijun Zhou, Yingjie Yu
A novel polymerized crystalline colloidal array (PCCA) sensing material for the detection of urine glucose was developed by embedding a two-dimensional (2-D) polystyrene crystalline colloidal array (CCA) in 3-acrylamidophenylboronic acid (3-APBA)-functionalized hydrogel. After adjusting the cross-linker concentration, this material showed significant sensitivity for glucose under lab conditions, the particle spacing of the PCCA changed from 917 to 824 nm (93 nm) within 3 min as the glucose concentration increased from 0 to 10 mM, and the structural color of the PCCA changed from red through orange, to green, and finally, to cyan...
September 28, 2016: Analytical and Bioanalytical Chemistry
J F C Loo, H C Kwok, C C H Leung, S Y Wu, I L G Law, Y K Cheung, Y Y Cheung, M L Chin, P Kwan, M Hui, S K Kong, H P Ho
Sepsis by bacterial infection causes high mortality in patients in intensive care unit (ICU). Rapid identification of bacterial infection is essential to ensure early appropriate administration of antibiotics to save lives of patients, yet the present benchtop molecular diagnosis is time-consuming and labor-intensive, which limits the treatment efficiency especially when the number of samples to be tested is extensive. Therefore, we hereby report a microfluidic platform lab-on-a-disc (LOAD) to provide a sample-to-answer solution...
September 2, 2016: Biosensors & Bioelectronics
Bernhard Wolfrum, Enno Kätelhön, Alexey Yakushenko, Kay J Krause, Nouran Adly, Martin Hüske, Philipp Rinklin
Micro- and nanofabriation technologies have a tremendous potential for the development of powerful sensor array platforms for electrochemical detection. The ability to integrate electrochemical sensor arrays with microfluidic devices nowadays provides possibilities for advanced lab-on-a-chip technology for the detection or quantification of multiple targets in a high-throughput approach. In particular, this is interesting for applications outside of analytical laboratories, such as point-of-care (POC) or on-site water screening where cost, measurement time, and the size of individual sensor devices are important factors to be considered...
September 20, 2016: Accounts of Chemical Research
Akilan Palanisami, Shazia Khan, Sultan Sibel Erdem, Tayyaba Hasan
Fever is one of the most common symptoms of illness in infants and represents a clinical challenge due to the potential for serious bacterial infection. As delayed treatment for these infections has been correlated with increased morbidity and mortality, broad-spectrum [Formula: see text]-lactam antibiotics are often prescribed while waiting for microbiological lab results (1-3 days). However, the spread of antibiotic resistance via the [Formula: see text]-lactamase enzyme, which can destroy [Formula: see text]-lactam antibiotics, has confounded this paradigm; empiric antibiotic regimens are increasingly unable to cover all potential bacterial pathogens, leaving some infants effectively untreated until the pathogen is characterized...
2016: IEEE Journal of Translational Engineering in Health and Medicine
François Patou, Fatima AlZahra'a Alatraktchi, Claus Kjægaard, Maria Dimaki, Jan Madsen, Winnie E Svendsen
The association of smart mobile devices and lab-on-chip technologies offers unprecedented opportunities for the emergence of direct-to-consumer in vitro medical diagnostics applications. Despite their clear transformative potential, obstacles remain to the large-scale disruption and long-lasting success of these systems in the consumer market. For instance, the increasing level of complexity of instrumented lab-on-chip devices, coupled to the sporadic nature of point-of-care testing, threatens the viability of a business model mainly relying on disposable/consumable lab-on-chips...
September 3, 2016: Diagnostics
Jayan Ozhikandathil, Simona Badilescu, Muthukumaran Packirisamy
Lab-on-chip technology is attracting great interest due to its potential as miniaturized devices that can automate and integrate many sample-handling steps, minimize consumption of reagent and samples, have short processing time and enable multiplexed analysis. Microfluidic devices have demonstrated their potential for a broad range of applications in life sciences, including point-of-care diagnostics and personalized medicine, based on the routine diagnosis of levels of hormones, cancer markers, and various metabolic products in blood, serum, etc...
August 27, 2016: Journal of Neural Transmission
Yildiz Uludag, Fehmi Narter, Erkin Sağlam, Güzin Köktürk, M Yağmur Gök, Mete Akgün, Serkan Barut, Sinan Budak
Recent advances in the area of biosensor technology and microfluidic applications have enabled the miniaturisation of the sensing platforms. Here we describe a new integrated and fully automated lab-on-a-chip-based biosensor device prototype (MiSens) that has potential to be used for point-of-care cancer biomarker testing. The key features of the device include a new biochip, a device integrated microfluidic system and real-time amperometric measurements during the flow of enzyme substrate. For ease of use, a new plug and play type sensor chip docking station has been designed...
November 2016: Analytical and Bioanalytical Chemistry
Marina Labor, Žarko Vrbica, Ivan Gudelj, Slavica Labor, Davor Plavec
BACKGROUND: COPD-6™ is a lung function testing device for a rapid pre-spirometry testing to screen-out at-risk individuals not having COPD and indicating those at risk. The aim of this study was to validate COPD-6™ lung function testing (index test) in general practice in discriminating patients with COPD out of the population at risk - smokers/ex-smokers with no previous diagnosis of COPD, using measurements at tertiary care as reference standard. METHODS: Consecutive 227 subjects (115 women, 185 smokers/42 ex-smokers, ≥20 pack-years) with no previous diagnosis of COPD, aged 52...
2016: BMC Family Practice
Chad D Meyerhoefer, Susan A Sherer, Mary E Deily, Shin-Yi Chou, Lizhong Peng, Tianyan Hu, Marion Nihen, Michael Sheinberg, Donald Levick
OBJECTIVE: To determine the effect of availability of clinical information from an integrated electronic health record system on pregnancy outcomes at the point of care. MATERIALS AND METHODS: We used provider interviews and surveys to evaluate the availability of pregnancy-related clinical information in ambulatory practices and the hospital, and applied multiple regression to determine whether greater clinical information availability is associated with improvements in pregnancy outcomes and changes in care processes...
August 18, 2016: Journal of the American Medical Informatics Association: JAMIA
R Geiger, D M Smith, S J Little, S R Mehta
OBJECTIVES: The GeneXpert(®) CT/NG (Cepheid, Sunnyvale, CA) assay is a point-of-care (POC) molecular diagnostic assay designed to rapidly test for the presence of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC). However, the test is only approved for vaginal swabs, urine, and endocervical swabs. Here, we performed an evaluation of the GeneXpert(®) CT/NG assay to detect the presence of CT and GC on male pharyngeal and rectal swabs. METHODS: Men who have sex with men participating in an HIV and Sexually Transmitted Infection (STI) screening program providing consent were enrolled into the study...
2016: Austin J HIV AIDS Res
Tae-Hyeong Kim, Vijaya Sunkara, Juhee Park, Chi-Ju Kim, Hyun-Kyung Woo, Yoon-Kyoung Cho
A lab-on-a-disc is a unique microfluidic platform that utilizes centrifugal force to pump liquids. This offers many benefits for point-of-care devices because it eliminates the need for connections to multiple pumps and complex tubing connections. A wide range of applications including clinical chemistry, immunoassay, cell analysis, and nucleic acid tests could be demonstrated on a spinning disc. To enable the performance of assays in a fully integrated and automated manner, the robust actuation of integrated valves is a prerequisite...
October 7, 2016: Lab on a Chip
Michaela C Pongratz, Hannah K Junge, Barbara Riond, Colin C Schwarzwald
OBJECTIVE: To assess the accuracy and reliability of a point-of-care (POC) triglyceride analyzer and to establish reference intervals for blood ([TRIG]POC/WB ) and plasma triglyceride concentrations ([TRIG]POC/PL ) in horses, ponies, and donkeys. DESIGN: Prospective study. SETTING: University teaching hospital. ANIMALS: 120 adult healthy equids (78 horses and ponies, 42 donkeys) and 79 equids suffering from hypertriglyceridemia (73 horses and ponies, 6 donkeys)...
September 2016: Journal of Veterinary Emergency and Critical Care
Despina Moschou, Louise Greathead, Panagiotis Pantelidis, Peter Kelleher, Hywel Morgan, Themistoklis Prodromakis
Lab-on-a-Chip (LoC) technology has the potential to revolutionize medical Point-of-Care diagnostics. Currently, considerable research efforts are focused on innovative production technologies that will make commercial upscaling of lab-on-chip products financially viable. Printed circuit board (PCB) manufacturing techniques have several advantages in this field. In this paper we focus on transferring a complete IFN-γ enzyme-linked immune-sorbent assay (ELISA) onto a commercial PCB electrochemical biosensing platform, We adapted a commercially available ELISA to detect the enzyme product TMB/H2O2 using amperometry, successfully reproducing the colorimetry-obtained ELISA standard curve...
December 15, 2016: Biosensors & Bioelectronics
Noriyuki Kadoya, Yujiro Nakajima, Masahide Saito, Yuki Miyabe, Masahiko Kurooka, Satoshi Kito, Yukio Fujita, Motoharu Sasaki, Kazuhiro Arai, Kensuke Tani, Masashi Yagi, Akihisa Wakita, Naoki Tohyama, Keiichi Jingu
PURPOSE: To assess the accuracy of the commercially available deformable image registration (DIR) software for thoracic images at multiple institutions. METHODS AND MATERIALS: Thoracic 4-dimensional (4D) CT images of 10 patients with esophageal or lung cancer were used. Datasets for these patients were provided by DIR-lab ( and included a coordinate list of anatomic landmarks (300 bronchial bifurcations) that had been manually identified. Deformable image registration was performed between the peak-inhale and -exhale images...
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
Li-Ju Wang, Rongrong Sun, Tina Vasile, Yu-Chung Chang, Lei Li
We present an optical sensing platform on a smartphone for high-throughput screening immunoassays. For the first time, a designed microprism array is utilized to achieve a one-time screening of 64 samples. To demonstrate the capability and the reliability of this optical sensing platform on smartphone, human interleukin 6 (IL-6) protein and six types of plant viruses are immunoassayed. The ability of quantification is shown by a sigmoidal dose-response curve fitting to analyze IL-6 protein. The accuracy in measuring the concentrations of IL-6 protein achieves 99...
August 16, 2016: Analytical Chemistry
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